Carrier Is Synthetic Polymer Patents (Class 435/180)
  • Patent number: 11661593
    Abstract: Provided herein are methods for purifying recombinant A Disintegrin-like and Metallopeptidase with Thrombospondin Type 1 Motif 13 (ADAMTS13) protein from a sample. The method comprises enriching for ADAMTS13 protein by chromatographically contacting the sample with hydroxyapatite under conditions that allow ADAMTS13 protein to appear in the eluate or supernatant from the hydroxylapatite. The methods may further comprise tandem chromatography with a mixed mode cation exchange/hydrophobic interaction resin that binds ADAMTS13 protein. Additional optional steps involve ultrafiltration/diafiltration, anion exchange chromatography, cation exchange chromatography, and viral inactivation. Also provided herein are methods for inactivating virus contaminants in protein samples, where the protein is immobilized on a support. Also provided herein are compositions of ADAMTS13 prepared according to said methods.
    Type: Grant
    Filed: December 16, 2014
    Date of Patent: May 30, 2023
    Inventors: Meinhard Hasslacher, Christian Fiedler, Christa Mayer, Artur Mitterer
  • Patent number: 11653659
    Abstract: Acidified milk product, which has a pH of between 3.0 and 5.0 and a content of lactose of at least 1.5 mg/ml, wherein the product contains a lactase, which retains its activity at a pH of 5.0 and a temperature of 37° C. at a level of at least 5% as compared to its activity at the optimum pH of the lactase.
    Type: Grant
    Filed: June 7, 2017
    Date of Patent: May 23, 2023
    Assignee: Chr. Hansen A/S
    Inventors: Soeren Ng Riis, Vojislav Vojinovic, Christian Gilleladen
  • Patent number: 11608513
    Abstract: The present invention relates to an immobilized capping enzyme, preferably an immobilized Vaccinia virus capping enzyme. Furthermore, the present invention relates to an immobilized cap-specific nucleoside 2?-O-methyltransferase, preferably an immobilized Vaccinia virus cap-specific nucleoside 2?-O-methyltransferase. Moreover, the present invention relates to a method for immobilizing said enzymes and to a method of using said enzymes for the addition of a 5?-cap structure to RNAs. Moreover, the present invention relates to an enzyme reactor for performing the capping reaction using said immobilized enzymes and the subsequent separation of the 5?-capped RNA product. In addition, the present invention relates to a kit comprising the capping enzyme and/or the cap-specific nucleoside 2?-O-methyltransferase.
    Type: Grant
    Filed: May 30, 2016
    Date of Patent: March 21, 2023
    Assignee: CureVac SE
    Inventors: Tilmann Roos, Benyamin Yazdan Panah, Markus Conzelmann, Andreas Thess, Dominik Buob, Martin Kunze, Veronika Wagner
  • Patent number: 11559560
    Abstract: Provided is a modified bacteriophage capable of infecting a target bacterium, which bacteriophage includes an ?/? small acid-soluble spore protein (SASP) gene encoding a SASP which is toxic to the target bacterium, wherein the SASP gene is under the control of a constitutive promoter which is foreign to the bacteriophage and the SASP gene.
    Type: Grant
    Filed: September 12, 2018
    Date of Patent: January 24, 2023
    Inventors: Heather Fairhead, Adam Wilkinson, Sarah Holme, Katy Pitts, Alison Jackson
  • Patent number: 11332702
    Abstract: A probiotic-alcoholic beverage composition comprising Bacillus coagulans is disclosed herein wherein the said spore or vegetative cell of Bacillus coagulans exhibited high recovery, tolerability, compatibility and viability of spores and vegetative cells after brewing. Methods for brewing an alcoholic beverage composition comprising Bacillus coagulans wherein said spore or bacterium is added during pre-fermentation, during fermentation and post fermentation stages are also disclosed.
    Type: Grant
    Filed: September 20, 2018
    Date of Patent: May 17, 2022
    Inventors: Muhammed Majeed, Kalyanam Nagabhushanam, Sivakumar Arumugam, Furqan Ali, Shaheen Majeed, Kirankumar Beede
  • Patent number: 11298680
    Abstract: A retention media for removing substances from water that includes an aggregate; at least one granular adsorbing material capable of adsorbing nutrients and metals; and a psyllium, wherein the psyllium performs as a binder to adhere the at least one granular adsorbing material to the aggregate to form a high permeability media mix is disclosed. A method of manufacturing a media mix is disclosed that includes creating a dry mixture of aggregate, at least one granular absorbing material, and a psyllium; and saturating the dry mixture with water to create a bond between the at least one granular absorbing material, and the aggregate via the psyllium thereby forming a high permeability media mix (HPMM). A method of using a media mix to reduce concentration of substances in water includes installing materials over granular adsorbing material, aggregate; and psyllium to reduce concentration of phosphorous or nitrogen or metals in the water.
    Type: Grant
    Filed: August 14, 2017
    Date of Patent: April 12, 2022
    Inventors: Allen P. Davis, Travis Ostrom, Charles White
  • Patent number: 11274227
    Abstract: A novel omniphobic surface coating is disclosed that provides both high oil-repellency and high-water repellency to the coated surface. The coating may contain perfluoropolyether (PFPE) or a similar fluorinated or perfluorinated polymer, as well as a hardener and a catalyst. One or more surfactants or viscosifiers may also be added. Further, the coating may contain one or more functional additives, including, but not limited to, coloring agents, anti-corrosive agents, anti-fouling agents, water-repellent agents, and/or oil-repellent agents. Methods for formulating the novel omniphobic surface coating are also described. Such methods include preparing a first part of the coating, which may contain, at minimum, a polymer such as PFPE or a similar fluorinated or perfluorinated polymer, and a suitable solvent; preparing a second part of the coating, which is a water-free system that may contain, at minimum, a hardener and a catalyst; and combining the first part and the second part together.
    Type: Grant
    Filed: August 6, 2019
    Date of Patent: March 15, 2022
    Assignee: Oceanit Laboratories, Inc.
    Inventors: Ganesh Kumar Arumugam, Vinod P. Veedu, Matthew Nakatsuka
  • Patent number: 11066353
    Abstract: The present invention relates to fluorocarbon vectors for the delivery of antigens to immunoresponsive target cells. It further relates to fluorocarbon vector-antigen constructs and the use of such vectors associated with antigens as vaccines and immunotherapeutics in animals.
    Type: Grant
    Filed: May 23, 2014
    Date of Patent: July 20, 2021
    Assignee: Altimmune UK Ltd
    Inventors: Dominique Bonnet, Carlton B. Brown, Bertrand V. Georges, Philip J. Sizer
  • Patent number: 11071210
    Abstract: An etching composition for etching an electrically conductive layer structure for forming a conductor track is provided. The etching composition includes an etchant, a highly branched compound and optionally a solvent. In addition, a method of etching an electrically conductive layer structure, a conductor track, an arrangement of at least two conductor tracks, and a component carrier are provided.
    Type: Grant
    Filed: January 14, 2019
    Date of Patent: July 20, 2021
    Assignee: AT&S Austria Technologie & Systemtechnik Aktiengesellschaft
    Inventors: Jolanta Klocek, Thomas Krivec
  • Patent number: 10508977
    Abstract: A wash solution for remediating lubricant contamination in cytological specimens includes a Tris-based alkaline buffer solution. The buffer solution may be prepared in methanol, and may include a proton-rich protein, or a basic amino acid, such as arginine, lysine, or guanidine. A method for remediating a specimen contaminated with lubricant includes using the wash solution and/or adding a salt to the final vial prior to preparing the microscope slide. The method may include adding the wash solution to the specimen, centrifuging the specimen and wash solution to form a supernatant and cell pellet, decanting the supernatant, and adding a preservative solution to the cell pellet. The method may further include adding salt to a vial containing the preservative solution and cell pellet.
    Type: Grant
    Filed: November 21, 2016
    Date of Patent: December 17, 2019
    Assignee: HOLOGIC, INC.
    Inventors: Erin Coffman, Steven Hecht, Paul MacLean
  • Patent number: 10364455
    Abstract: The invention generally relates to compositions for maximizing capture of affinity-labeled molecules on solid supports. The disclosed methods and compositions were developed to maximize depletion of ribosomal RNA from total RNA samples, which is useful to improve the quality of RNA preparations used for applications such as massively parallel sequencing. The RNA depletion method is based on using long affinity-labeled RNA molecules that are complementary to all or part of the target ribosomal RNAs, as subtractive hybridization probes.
    Type: Grant
    Filed: September 27, 2013
    Date of Patent: July 30, 2019
    Assignee: BIOO Scientific Corporation
    Inventors: Marianna Goldrick, Lance Lepovitz, Masoud Toloue
  • Patent number: 10131899
    Abstract: A composition useful for cell capture, the composition comprising a solid substrate on which is affixed a patterned polymer, and a cell-targeting agent attached to said patterned polymer, wherein said cell-targeting agent is exposed. Also described is a method for the preparation of the cell capturing composition, as well as flow through devices in which the cell capturing composition is incorporated. Further described is a method of capturing cells by contacting the cell-capturing composition with a liquid or gaseous sample containing cells. The method for capturing cells may also be a method for testing for the presence of one or more classes or species of cells or cellular organisms in a liquid or gaseous sample.
    Type: Grant
    Filed: March 17, 2014
    Date of Patent: November 20, 2018
    Assignee: UT-BATTELLE, LLC
    Inventors: Ryan Robert Hansen, Scott T. Retterer, Bradley Steward Lokitz, Jennifer L. Morrell-Falvey, Juan Pablo Hinestrosa Salazar, Jamie Michael Messman, Sidney Michael Kilbey, II, John Francis Ankner
  • Patent number: 10118834
    Abstract: Monodisperse colloidal nanocrystal clusters of magnetite (Fe3O4) with tunable sizes from about thirty to about three hundred nanometers have been synthesized using a high-temperature hydrolysis process. The colloidal nanocrystal clusters are capped with polyelectrolytes, and highly water soluble. Each cluster is composed of many single magnetite crystallites, thus retaining the superparamagnetic behavior at room temperature. The combination of superparamagnetic property, high magnetization, and high water dispersibility makes the colloidal nanocrystal clusters ideal candidates for various important biomedical applications such as drug delivery and bioseparation. The present invention is further directed to methods for forming colloidal photonic crystals from both aqueous and nonaqueous solutions of the superparamagnetic colloidal nanocrystal clusters with an external magnetic field applied thereto.
    Type: Grant
    Filed: April 26, 2008
    Date of Patent: November 6, 2018
    Inventors: Yadong Yin, Jianping Ge
  • Patent number: 10040032
    Abstract: A complex nanofiltration membrane comprising a substrate and a separating layer, wherein the separating layer is an oxidant-treated, crosslinked network structure formed from a hydroxyl-containing polymer, a thiol-containing silane coupling agent and a crosslinking agent, is disclosed. Also disclosed are a process for preparing the complex nanofiltration membrane and use of the complex nanofiltration membrane in water treatment.
    Type: Grant
    Filed: July 22, 2015
    Date of Patent: August 7, 2018
    Inventors: Yang Zhang, Yiqun Liu, Guoyuan Pan, Hao Yan, Jian Xu, Min Guo
  • Patent number: 10012647
    Abstract: A process for depleting host cells from a xenograft of human cells on a murine host is disclosed. The process includes fragmenting the xenograft, subjecting the sample to antibodies specific for a murine CD9 epitope coupled to a detection means, depleting the cell suspension from cells bound by the CD9-antibodies using the detection means, and collecting the cells not bound by the CD9-antibodies as target cells.
    Type: Grant
    Filed: July 15, 2015
    Date of Patent: July 3, 2018
    Assignee: Miltenyi Biotec, GmbH
    Inventors: Andreas Bosio, Olaf Hardt, David Agorku
  • Patent number: 9938373
    Abstract: Disclosed herein are methods of electrospinning poly(glycerol sebacate) (PGS) which allow stable PGS fibers and fibrous PGS constructs, scaffolds and grafts to be formed. In one example, a disclosed method includes generating PGS fibers by blending PGS prepolymer with a heat resistant synthetic carrier polymer, wherein the blend is electrospun into micro- or nano-fibers, and the PGS prepolymer is cross-linked into PGS with heat without using chemical cross-linkers. In another example, a disclosed method includes electrospinning a PGS and gelatin blend, wherein the PGS and gelatin composition are cross-linked by heat curing without using chemical cross-linkers. In another example, the method includes preparing an electrospinning precursor solution comprising blending PGS prepolymer with poly(lactic-co-glycolic acid) (PLGA) and a chemical cross-linker; electrospinning the prepared blend; and exposing the electrospun blend to an organic solvent to remove the PLGA.
    Type: Grant
    Filed: December 20, 2013
    Date of Patent: April 10, 2018
    Assignee: University of Pittsburgh—Of the Commonwealth System of Higher Education
    Inventors: Yadong Wang, Eric M. Jeffries, Robert A. Allen
  • Patent number: 9925517
    Abstract: The present invention is related to a selective adsorption process aimed at reducing the content of nitrogen organic compounds (NOC's) that is present in hydrotreating loads (HDT) for the production of ultra-low-sulfur diesel (ULSD below 15 ppm), which is carried out at ambient temperature, atmospheric pressure, without hydrogen, using adsorbing materials with organic metal structure MIL-101-Cr-MX+ (MOF MIL-101-Cr-MX+), where MX+ can be any metal cation such as Mg2+, Al3+ or Ti4+. Likewise, the present invention considers the preparation of extrudates and the regeneration of the employed MOF adsorbent materials. In this sense, it is worth noting that the diesel HDT loads to which the present invention is referred to are hydrocarbon currents with distillation temperatures ranging from 150 to 400° C.
    Type: Grant
    Filed: May 13, 2016
    Date of Patent: March 27, 2018
    Assignee: Instituto Mexicano De Petroleo
    Inventors: Rodolfo Juventino Mora Vallejo, J. Ascencion Montoya De La Fuente, Georgina Cecilia Laredo Sanchez, Edith Meneses Ruiz, J. Jesus Castillo Munguia, Beatriz Zapata Rendon
  • Patent number: 9890176
    Abstract: The present invention relates to compounds useful for the treatment or prevention of bacteria infections. These compounds have formula I: The invention also provides processes for making the compounds described herein. Furthermore, the present invention provides a composition comprising the compounds described herein, and a pharmaceutically acceptable carrier, adjuvant, or vehicle. The present invention also provides methods of treating or preventing bacteria infection in a subject, comprising administering to the subject an effective amount of the compound or the composition described herein.
    Type: Grant
    Filed: March 12, 2014
    Date of Patent: February 13, 2018
    Assignee: Vertex Pharmaceuticals Incorporated
    Inventors: Evelyne Dietrich, Carl Poisson, Michel Gallant, Stephanie Lessard, Bingcan Liu, Sanjoy Kumar Das, Yeeman Ramtohul, Thumkunta Jagadeeswar Reddy, Julien Martel, Frederic Vallee, Jean-Francois Lévesque
  • Patent number: 9849173
    Abstract: The present disclosure provides methods for preparing vesicles. In some embodiments, the methods involve providing a molten mixture of vesicle forming lipids and then adding the molten mixture to an aqueous solution comprising an antigen such that antigen-containing vesicles are formed. In other embodiments, the methods involve providing a lyophilized lipid product and rehydrating the lyophilized lipid product with an aqueous solution comprising an antigen such that antigen-containing vesicles are formed. The lyophilized lipid product is prepared by melting vesicle-forming lipids to produce a molten lipid mixture and then lyophilizing the molten lipid mixture. The present disclosure also provides antigen-containing vesicle formulations prepared using these methods. The present disclosure also provides kits that include a lyophilized lipid product in a first container and an aqueous solution comprising an antigen in a second container.
    Type: Grant
    Filed: July 6, 2010
    Date of Patent: December 26, 2017
    Assignee: Variation Biotechnologies Inc.
    Inventors: David E. Anderson, Andrei Ogrel
  • Patent number: 9822330
    Abstract: There is provided a new material that can form a finer pattern and can be applied to adsorption/adhesion control of various cell species, proteins, viruses, and the like without the limitation of the light source. A light-degradable material comprising: a moiety that is capable of bonding to a surface of a substrate through a siloxane bond; and a structural unit of Formula (2-a) and/or Formula (2-b): (where R2 to R4 are saturated linear alkyl groups; X is a hydrogen atom or an alkyl group; Z is a carbanion or a sulfo anion; Q is an ester bond group, a phosphodiester bond group, an amido bond group, an alkylene group, or an phenylene group or a combination of these divalent groups; m1 is an integer of 1 to 200, and n is an integer of 1 to 10).
    Type: Grant
    Filed: October 11, 2013
    Date of Patent: November 21, 2017
    Inventors: Takahiro Kishioka, Shigeo Kimura, Yoshiomi Hiroi, Yuki Usui, Hiromi Kitano, Tadashi Nakaji, Makoto Gemmei
  • Patent number: 9675746
    Abstract: Extracorporeal blood treatment is performed daily on a large number of patients by use of for example a dialysis filter, plasma filter or a centrifuge. The purpose of the treatment is to separate minor components and molecules in a liquid or in the blood from larger ones, for example in connection with different disease conditions or with a view to extracting blood plasma, target substances such as blood components or molecules from for example blood donors. According to the present invention two separation steps are used together with a solution or a suspension containing at least one component, which specifically may bind to the component or the blood component to be specifically reduced, or to be refined/extracted during the treatment or during the blood treatment.
    Type: Grant
    Filed: October 18, 2010
    Date of Patent: June 13, 2017
    Assignee: GLYCOREX AB
    Inventor: Kurt G. I. Nilsson
  • Patent number: 9657132
    Abstract: The present disclosure provides amine-modified polymer foams, which may be used for wound dressing materials. For example, the modified materials can include a covalently attached molecule comprising free amine groups. Such amine groups can be used, for instance, to conjugate biologically active polypeptides and/or linkers. Methods for using modified polymers are also provided.
    Type: Grant
    Filed: October 24, 2013
    Date of Patent: May 23, 2017
    Assignee: KCI Licensing, Inc.
    Inventors: Douglas Philip Hanson, James Courage
  • Patent number: 9523109
    Abstract: The invention provides a method for preparing a peptide for improving brain function, comprising hydrolyzing milk casein with an enzymatic catalyst comprising a protease to produce a hydrolysate comprising: (i) a peptide consisting of the amino acid sequence shown in SEQ ID NO: 1 or a variant thereof; (ii) a peptide consisting of the amino acid sequence shown in SEQ ID NO: 2 or a variant thereof; or (iii) a mixture of the peptides of (i) and (ii), wherein the production yield of each of the peptides is 2% or more, or the total production yield of the mixture is 10% or more. The invention also provides a food or drink or a pharmaceutical composition containing the hydrolysate.
    Type: Grant
    Filed: June 13, 2012
    Date of Patent: December 20, 2016
    Assignee: CALPIS CO., LTD.
    Inventors: Naoto Uchida, Hiroaki Goto, Kazuhito Ohsawa, Kohji Ohki
  • Patent number: 9371563
    Abstract: The present invention relates to compositions and methods for detection and quantification of individual target molecules in biomolecular samples. In particular, the invention relates to coded, labeled probes that are capable of binding to and identifying target molecules based on the probes' label codes. Methods of making and using such probes are also provided. The probes can be used in diagnostic, prognostic, quality control and screening applications.
    Type: Grant
    Filed: March 11, 2013
    Date of Patent: June 21, 2016
    Assignees: NanoString Technologies, Inc., The Institute for Systems Biology
    Inventors: Gary K. Geiss, Sean M. Ferree, Philippa Jane Webster, Krassen M. Dimitrov
  • Patent number: 9358326
    Abstract: Substrates, optionally coated with an undercoating, having grafted thereto one or more non-fouling materials are described herein. The non-fouling, polymeric material can be grafted to a variety of functionalized substrate materials, particularly polymeric substrates and/or polymeric undercoatings immobilized on a substrate. The compositions described herein are highly resistant protein absorption, particularly in complex media and retain a high degree of non-fouling activity over long periods of time. The compositions described herein may also demonstrate antimicrobial and/or anti-thrombogenic activity. The non-fouling material can be grafted to a functionalized substrate, or optionally from an undercoating on the substrate, preferably without significantly affecting the mechanical and/or physical properties of the substrate material.
    Type: Grant
    Filed: November 8, 2012
    Date of Patent: June 7, 2016
    Inventors: Zheng Zhang, Chad Huval, William Shannan O'Shaughnessey, Michael Hencke, Trevor Squier, Jun Li, Michael Bouchard, Christopher R. Loose
  • Patent number: 9168680
    Abstract: One or more techniques for nano structure fabrication are provided. In an embodiment, an apparatus for manufacturing a nano structure is disclosed. The apparatus includes a stamp having a line pattern on a surface thereof that comprises a plurality of protrusions, a die configured to hold a substrate thereon, and a mechanical processing unit configured to press the plurality of protrusions of the stamp against the substrate with a predetermined pressure so as to form at least one channel pore therebetween.
    Type: Grant
    Filed: July 27, 2012
    Date of Patent: October 27, 2015
    Inventor: Kwangyeol Lee
  • Patent number: 9168574
    Abstract: A thixotropic gel suitable for use in subsurface bioremediation is provided along with a process of using the gel. The thixotropic gel provides a non-migrating injectable substrate that can provide below ground barrier properties. In addition, the gel components provide for a favorable environment in which certain contaminants are preferentially sequestered in the gel and subsequently remediated by either indigenous or introduced microorganisms.
    Type: Grant
    Filed: June 1, 2012
    Date of Patent: October 27, 2015
    Inventors: Brian D. Riha, Brian B. Looney
  • Publication number: 20150140604
    Abstract: Disclosed in the present invention are an immobilized cycloaliphatic peptide acyltransferase and a preparation method and use thereof. The cycloaliphatic peptide acyltransferase is immobilized on a carrier; the cycloaliphatic peptide acyltransferase is derived from natural or artificial mutants or variants thereof, or can be obtained by introducing a foreign cyclic acyltransferase gene and transforming thereafter; the material of the carrier is selected from an inorganic carrier or a polypropylene resin carrier. Also disclosed in the present invention are the preparation method for the immobilized cycloaliphatic peptide acyltransferase and uses thereof.
    Type: Application
    Filed: May 10, 2013
    Publication date: May 21, 2015
    Inventors: Shidong Liu, Zhaoli Zhang, Xiaoming Ji
  • Patent number: 9011895
    Abstract: A method of forming and preserving a bioremodelable, biopolymer scaffold material by subjecting animal tissue to chemical and mechanical processing. In addition to skin tissue, another source of EBM is a blood vessel. EBM may be used for hernia repair, colon, rectal, vaginal and or urethral prolapse treatment; pelvic floor reconstruction; muscle flap reinforcement; lung tissue support; rotator cuff repair or replacement; periosteum replacement; dura repair; pericardial membrane repair; soft tissue augmentation; intervertebral disk repair; and periodontal repair. EBM may also be used as a urethral sling, laminectomy barrier or spinal fusion device.
    Type: Grant
    Filed: November 18, 2008
    Date of Patent: April 21, 2015
    Assignee: TEI Biosciences, Inc.
    Inventors: Jianwu Dai, Eugene Bell, Vladimir Russakovsky
  • Publication number: 20150104782
    Abstract: A method for CO2 capture may include operating a packed reactor comprising a reaction chamber containing packing including immobilized enzymes, by contacting a CO2 containing gas with a liquid solution in the reaction chamber to produce an ion-loaded solution and a CO2 depleted gas by an enzymatically catalyzed hydration reaction; monitoring enzyme activity of the immobilized enzymes; at a low enzyme activity threshold (i) stopping operation in the packed reactor, and (ii) replenishing the enzymatic activity by providing an enzyme replenishing solution into the packed reactor to contact the packing and provide a replenishing amount of the immobilized enzymes; and recommencing operation in the packed reactor for CO2 capture using the replenished immobilized enzymes. A corresponding system may include a packed reactor and an in situ enzyme supply device for supplying active enzyme within the reactor. The enzyme supply device may include spray nozzles with various configurations.
    Type: Application
    Filed: May 16, 2013
    Publication date: April 16, 2015
    Applicant: C02 Solutions Inc.
    Inventors: Eric Madore, Sylvie Fradette, Jeremy Lawson
  • Patent number: 8950068
    Abstract: A mountable device includes a bio-compatible structure embedded in a polymer that defines at least one mounting surface. The bio-compatible structure has a first side defined by a first layer of bio-compatible material, a second side defined by a second layer of bio-compatible material, an electronic component, and a conductive pattern that defines sensor electrodes. A portion of the second layer of bio-compatible material is removed by etching to create at least one opening in the second side in which the sensor electrodes are exposed. The etching further removes a portion of the first layer of bio-compatible material so as to create at least one opening in the first side that is connected to the at least opening in the second side. With this arrangement of openings, analytes can reach the sensor electrodes from either the first side or the second side of the bio-compatible structure.
    Type: Grant
    Filed: September 20, 2013
    Date of Patent: February 10, 2015
    Assignee: Google Inc.
    Inventor: James Etzkorn
  • Patent number: 8951761
    Abstract: The disclosed matter relates to immobilized enzymes and methods of use thereof.
    Type: Grant
    Filed: June 13, 2006
    Date of Patent: February 10, 2015
    Assignee: DSM Nutritional Products AG
    Inventors: Jaroslav A. Kralovec, Weijie Wang
  • Publication number: 20140377319
    Abstract: Devices and methods of altering protein concentrations in cerebrospinal fluid and/or brain interstitial fluid are disclosed. Devices include a support having at least one protease attached to the support. The device may further include a housing. Devices may be implantable for use in an in vivo active flow system or for use in an in vivo passive system. Devices may also be used in an ex vivo active flow system. Devices may also be used in a passive system to treat cerebrospinal fluid and/or brain interstitial fluid that is withdrawn, or in a passive system that is implanted surgically. Methods include contacting cerebrospinal fluid and/or brain interstitial fluid with a support including at least one protease attached to the support. Proteins contained in the cerebrospinal fluid and/or brain interstitial fluid are cleaved by the protease resulting in the reduction of protein in cerebrospinal fluid and/or brain interstitial fluid.
    Type: Application
    Filed: April 18, 2012
    Publication date: December 25, 2014
    Inventors: Eric C. Leuthardt, Daniel W. Moran, David Holtzman, Philip Bayly, David Brody, Ralph G. Dacey, Sam Fok, David Jeremiah Garrison, Guy M. Genin, Eriks Lusis, Rohit Pappu, Jingwei Xie, Greg Zipfel
  • Publication number: 20140370567
    Abstract: Dual-functional nonfouling surfaces and materials, methods for making dual-functional nonfouling surfaces and materials, and devices that include dual-functional nonfouling surfaces and materials. The dual-functional surfaces are nonfouling surfaces that resist non-specific protein adsorption and cell adhesion. The dual-functional surfaces and materials include covalently coupled biomolecules (e.g., target binding partners) that impart specific biological activity thereto. The surfaces and materials are useful in medical diagnostics, biomaterials and bioprocessing, tissue engineering, and drug delivery.
    Type: Application
    Filed: August 27, 2014
    Publication date: December 18, 2014
    Inventors: Shaoyi Jiang, Zheng Zhang, Shengfu Chen, Hana Vaisocherova
  • Patent number: 8911986
    Abstract: Temporary active coatings that are stabilized against inactivation by weathering are provided including a base associated with a chemically modified enzyme, and, optionally a first polyoxyethylene present in the base and independent of the enzyme. The coatings are optionally overlayered onto a substrate to form an active coating facilitating the removal of organic stains or organic material from food, insects, or the environment.
    Type: Grant
    Filed: April 29, 2011
    Date of Patent: December 16, 2014
    Assignees: Toyota Motor Engineering & Manufacturing North America, Inc., Toyota Motor Corporation
    Inventors: Hongfei Jia, Songtao Wu, Masahiko Ishii, Minjuan Zhang
  • Patent number: 8906686
    Abstract: Neo-cartilage constructs suitable for implantation into a joint cartilage lesion in situ and a method for repair and restoration of function of injured, traumatized, aged or diseased cartilage. The construct comprises at least chondrocytes incorporated into a support matrix processed according to the algorithm comprising variable hydrostatic or atmospheric pressure or non-pressure conditions, variable rate of perfusion, variable medium composition, variable temperature, variable cell density and variable time to which the chondrocytes are subjected.
    Type: Grant
    Filed: April 28, 2006
    Date of Patent: December 9, 2014
    Assignee: Histogenics Corporation
    Inventors: Shuichi Mizuno, Akihiko Kusanagi, Laurence J. B. Tarrant, Toshimasa Tokuno, Robert Lane Smith
  • Publication number: 20140356410
    Abstract: The embodiments herein provide a biodegradable and biocompatible T-plate nano-composite implant with stem cells for treating and repairing broken bones, damaged tissues and torn ligaments. The implant comprises a polymeric matrix part comprising poly lactic glycolic acids (PLGA), a bioceramic part comprising hydroxyapatite (HAp) nanoparticles and an endometrial stem cell. The PLGA and HAp nanoparticles act as a matrix and reinforcing agents respectively. A method is provided for synthesizing the T-plate implant. The method comprises synthesizing hydroxyapatite (HAp) nanoparticles, poly lactic glycolic acids (PLGA) and HAp nano composite implant. The casting of the poly lactic glycolic acids (PLGA) and HAp nano composite are done in a mold to obtain a T-plate nano composite. An endometrial stem cell from an epithelial cell lining from uterus is isolated and cultured. The endometrial stem cells are implanted on the nano-composite implant.
    Type: Application
    Filed: June 10, 2014
    Publication date: December 4, 2014
    Inventors: Jafar Ai, Nastaran Elyasi far, Mahmoud Azami, Naghmeh Bahrami, Shima Tavakol
  • Patent number: 8895046
    Abstract: Adult autologous stem cells cultured on a porous, three-dimensional tissue scaffold-implant for bone regeneration by the use of a hyaluronan and/or dexamethasone to accelerate bone healing alone or in combination with recombinant growth factors or transfected osteogenic genes. The scaffold-implant may be machined into a custom-shaped three-dimensional cell culture system for support of cell growth, reservoir for peptides, recombinant growth factors, cytokines and antineoplastic drugs in the presence of a hyaluronan and/or dexamethasone alone or in combination with growth factors or transfected osteogenic genes, to be assembled ex vivo in a tissue incubator for implantation into bone tissue.
    Type: Grant
    Filed: October 9, 2007
    Date of Patent: November 25, 2014
    Assignee: Zimmer, Inc.
    Inventors: Zou Xuenong, Haisheng Li, Cody Bunger
  • Publication number: 20140341865
    Abstract: There is provided a dressing for treating a wound. The dressing for healing a wound can be useful in maintaining a moisture environment at a wound site using a biocompatible polymer scaffold, and effectively promoting healing of a wound by various growth factors secreted by skin cells or stem cells attached to the biocompatible polymer scaffold as well.
    Type: Application
    Filed: December 12, 2012
    Publication date: November 20, 2014
    Applicant: TEGO SCIENCE INC.
    Inventors: Saewha Jeon, Yun Hee Kim, Ho Yun Chung, Jin Hyun Choi, Hankyu Jang
  • Patent number: 8883441
    Abstract: A method for detecting and counting the microorganisms in a sample is described. The method comprises: a) selectively enriching the microorganism sought in the sample, b) inducing or activating at least one enzymatic activity of the microorganism, c) immunomagnetically concentrating the microorganism, d) fluorescently labeling the microorganism, and e) detecting and analyzing the fluorescence making possible the numeration or counting of the microorganisms sought by flow cytometry, filtration cytometry or fluorescence microscopy.
    Type: Grant
    Filed: October 1, 2003
    Date of Patent: November 11, 2014
    Assignee: Metis Biotechnologies
    Inventors: Bruno Vedrine, Aline Lachaise, Vincent Carre
  • Patent number: 8877496
    Abstract: The invention provides a method for transferring cells to carriers, including: (a) providing a hydrophobic cell culture container or a cell culture container coated with a hydrophobic material on a bottom thereof; (b) adding carriers which are more hydrophilic than the hydrophobic cell culture container or hydrophobic materials and a culture medium containing cells into the hydrophobic cell culture container or the cell culture container coated with the hydrophobic material on the bottom thereof; and (c) culturing the cells, wherein the cells attach to the carriers and grow.
    Type: Grant
    Filed: June 5, 2009
    Date of Patent: November 4, 2014
    Assignee: National Taiwan University
    Inventors: Chin-Hsiung Hsieh, Yi-You Huang
  • Publication number: 20140322786
    Abstract: Provided are polymer modified substrates which comprise a) a substrate, b) a binding layer covalently attached to the surface of the substrate and covering at least a part of this surface; and c) a polymer brush formed by a plurality of polymer chains, each of which is covalently attached at one of its terminals to the binding layer. Moreover, methods are provided, for the preparation of the polymer modified substrates by polymerizing vinyl group containing monomers, such as vinylphosphonates, on a binding layer provided on a substrate.
    Type: Application
    Filed: November 13, 2012
    Publication date: October 30, 2014
    Applicant: APCETH GMBH & CO KG
    Inventors: Frank Deubel, Bernhard Rieger, Stephan Salzinger, Ning Zhang
  • Patent number: 8835144
    Abstract: Dual-functional nonfouling surfaces and materials, methods for making dual-functional nonfouling surfaces and materials, and devices that include dual-functional nonfouling surfaces and materials. The dual-functional surfaces are nonfouling surfaces that resist non-specific protein adsorption and cell adhesion. The dual-functional surfaces and materials include covalently coupled biomolecules (e.g., target binding partners) that impart specific biological activity thereto. The surfaces and materials are useful in medical diagnostics, biomaterials and bioprocessing, tissue engineering, and drug delivery.
    Type: Grant
    Filed: June 29, 2009
    Date of Patent: September 16, 2014
    Assignee: University of Washington
    Inventors: Shaoyi Jiang, Zheng Zhang, Shengfu Chen, Hana Vaisocherova
  • Patent number: 8822550
    Abstract: The present invention provides a UCST-type thermoresponsive polymer compound which responds to a temperature under physiological conditions and has biofunctionality, and various uses thereof as a thermoresponsive material. Specifically, the invention provides a thermoresponsive material containing, as an active ingredient, a polymer compound represented by Formula (I) below or an addition salt thereof, the thermoresponsive material having an upper critical solution temperature in the range of 5 to 50° C. in an aqueous solution with a salt concentration of at least 1 mM and a pH in the range of 3 to 10.5: wherein m represents an integer of 10 or more; n represents a number satisfying 0.4?n?1; R1 represents hydrogen or succinyl; and R2 represents carbamoyl.
    Type: Grant
    Filed: March 22, 2011
    Date of Patent: September 2, 2014
    Assignee: Kyushu University, National University Corporation
    Inventors: Naohiko Shimada, Atsushi Maruyama
  • Patent number: 8802410
    Abstract: There is disclosed an analytical method and a sensor suitable for carrying out the method. More specifically, there is disclosed a method for preparing a mass sensitive chemical sensor capable of detecting binding analyte species to a surface comprising cells.
    Type: Grant
    Filed: March 20, 2010
    Date of Patent: August 12, 2014
    Assignee: Attana AB
    Inventors: Camilla Käck, Julien Saint-Guirons, Björn Ingemarsson, Kerstin Lundell
  • Patent number: 8796000
    Abstract: The invention is directed to enzyme preparations which are obtainable by providing enzyme immobilizates which comprise enzymes or microorganisms comprising enzymes immobilized on an inert support with a polyethersilicone coating obtained by hydrosilylation, to a process for preparing such enzyme preparations and to the use of enzyme preparations as an industrial biocatalyst.
    Type: Grant
    Filed: July 15, 2013
    Date of Patent: August 5, 2014
    Assignee: Evonik Degussa GmbH
    Inventors: Oliver Thum, Marion Ansorge-Schumacher, Lars Wiemann, Michael Ferenz, Matthias Naumann
  • Patent number: 8796009
    Abstract: A substrate or coating is provided that includes a protease with enzymatic activity toward a component of a biological stain. Also provided is a process for facilitating the removal of a biological stain is provided wherein an inventive substrate or coating including a protease is capable of enzymatically degrading of one or more components of the biological stain to facilitate biological stain removal from the substrate or said coating.
    Type: Grant
    Filed: June 21, 2010
    Date of Patent: August 5, 2014
    Assignees: Toyota Motor Engineering & Manufacturing North America, Inc., Toyota Motor Corporation
    Inventors: Hongfei Jia, Wei Song, Masahiko Ishii, Minjuan Zhang
  • Publication number: 20140212944
    Abstract: A series of MOF-based hierarchical porous material, namely IPD-mesoMOF-1˜9, based on nanoscale MOFs of MIL-100(Al, Fe, Cr, Sc and In), MIL-53(Al), HKUST-1, DUT-5, DUT-4, MIL-101(Cr), MIL-101NDC(Cr), MIL-101BPDC(Cr) and MIL-110 respectively, forming the permanent interparticle porosities by using close (or relatively close) packing, and preparation methods thereof. Modulated or functionalized IPD-mesoMOFs can be applied for gas adsorption and molecule separation (such as CH4- and CO2-adsorption, gasoline/diesel desulfurization and purification), catalyst loadings and molecular recognition/immobilization of biological macromolecules and enzymes.
    Type: Application
    Filed: April 6, 2014
    Publication date: July 31, 2014
    Inventors: Yunqi Tian, Yan Chen, Xun Liu
  • Patent number: 8778376
    Abstract: A copolymer comprising a block of an elastin pentapeptide and method of making and using the copolymer are provided.
    Type: Grant
    Filed: June 9, 2006
    Date of Patent: July 15, 2014
    Assignee: Advanced Cardiovascular Systems, Inc.
    Inventors: Syed F. A. Hossainy, Mikael Trollsas, Thierry Glauser, Yiwen Tang
  • Patent number: 8778333
    Abstract: The present invention relates to injectable compositions comprising biocompatible, hydrophilic, non-toxic and substantially spherical microspheres associated with stem cells useful for tissue construction and generation. The invention also relates to methods of tissue construction and generation, for the treatment of various tissue damage and defects, using the injectable compositions.
    Type: Grant
    Filed: January 16, 2008
    Date of Patent: July 15, 2014
    Assignee: Biosphere Medical, Inc.
    Inventors: Jean-Marie Vogel, Egisto Boschetti