Carrier Is Synthetic Polymer Patents (Class 435/180)
  • Patent number: 10364455
    Abstract: The invention generally relates to compositions for maximizing capture of affinity-labeled molecules on solid supports. The disclosed methods and compositions were developed to maximize depletion of ribosomal RNA from total RNA samples, which is useful to improve the quality of RNA preparations used for applications such as massively parallel sequencing. The RNA depletion method is based on using long affinity-labeled RNA molecules that are complementary to all or part of the target ribosomal RNAs, as subtractive hybridization probes.
    Type: Grant
    Filed: September 27, 2013
    Date of Patent: July 30, 2019
    Assignee: BIOO Scientific Corporation
    Inventors: Marianna Goldrick, Lance Lepovitz, Masoud Toloue
  • Patent number: 10131899
    Abstract: A composition useful for cell capture, the composition comprising a solid substrate on which is affixed a patterned polymer, and a cell-targeting agent attached to said patterned polymer, wherein said cell-targeting agent is exposed. Also described is a method for the preparation of the cell capturing composition, as well as flow through devices in which the cell capturing composition is incorporated. Further described is a method of capturing cells by contacting the cell-capturing composition with a liquid or gaseous sample containing cells. The method for capturing cells may also be a method for testing for the presence of one or more classes or species of cells or cellular organisms in a liquid or gaseous sample.
    Type: Grant
    Filed: March 17, 2014
    Date of Patent: November 20, 2018
    Assignee: UT-BATTELLE, LLC
    Inventors: Ryan Robert Hansen, Scott T. Retterer, Bradley Steward Lokitz, Jennifer L. Morrell-Falvey, Juan Pablo Hinestrosa Salazar, Jamie Michael Messman, Sidney Michael Kilbey, II, John Francis Ankner
  • Patent number: 10118834
    Abstract: Monodisperse colloidal nanocrystal clusters of magnetite (Fe3O4) with tunable sizes from about thirty to about three hundred nanometers have been synthesized using a high-temperature hydrolysis process. The colloidal nanocrystal clusters are capped with polyelectrolytes, and highly water soluble. Each cluster is composed of many single magnetite crystallites, thus retaining the superparamagnetic behavior at room temperature. The combination of superparamagnetic property, high magnetization, and high water dispersibility makes the colloidal nanocrystal clusters ideal candidates for various important biomedical applications such as drug delivery and bioseparation. The present invention is further directed to methods for forming colloidal photonic crystals from both aqueous and nonaqueous solutions of the superparamagnetic colloidal nanocrystal clusters with an external magnetic field applied thereto.
    Type: Grant
    Filed: April 26, 2008
    Date of Patent: November 6, 2018
    Assignee: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
    Inventors: Yadong Yin, Jianping Ge
  • Patent number: 10040032
    Abstract: A complex nanofiltration membrane comprising a substrate and a separating layer, wherein the separating layer is an oxidant-treated, crosslinked network structure formed from a hydroxyl-containing polymer, a thiol-containing silane coupling agent and a crosslinking agent, is disclosed. Also disclosed are a process for preparing the complex nanofiltration membrane and use of the complex nanofiltration membrane in water treatment.
    Type: Grant
    Filed: July 22, 2015
    Date of Patent: August 7, 2018
    Assignees: CHINA PETROLEUM & CHEMICAL CORPORATION, BEIJING RESEARCH INSTITUTE OF CHEMICAL INDUSTRY, CHINA PETROLEUM & CHEMICAL CORPORATION
    Inventors: Yang Zhang, Yiqun Liu, Guoyuan Pan, Hao Yan, Jian Xu, Min Guo
  • Patent number: 10012647
    Abstract: A process for depleting host cells from a xenograft of human cells on a murine host is disclosed. The process includes fragmenting the xenograft, subjecting the sample to antibodies specific for a murine CD9 epitope coupled to a detection means, depleting the cell suspension from cells bound by the CD9-antibodies using the detection means, and collecting the cells not bound by the CD9-antibodies as target cells.
    Type: Grant
    Filed: July 15, 2015
    Date of Patent: July 3, 2018
    Assignee: Miltenyi Biotec, GmbH
    Inventors: Andreas Bosio, Olaf Hardt, David Agorku
  • Patent number: 9938373
    Abstract: Disclosed herein are methods of electrospinning poly(glycerol sebacate) (PGS) which allow stable PGS fibers and fibrous PGS constructs, scaffolds and grafts to be formed. In one example, a disclosed method includes generating PGS fibers by blending PGS prepolymer with a heat resistant synthetic carrier polymer, wherein the blend is electrospun into micro- or nano-fibers, and the PGS prepolymer is cross-linked into PGS with heat without using chemical cross-linkers. In another example, a disclosed method includes electrospinning a PGS and gelatin blend, wherein the PGS and gelatin composition are cross-linked by heat curing without using chemical cross-linkers. In another example, the method includes preparing an electrospinning precursor solution comprising blending PGS prepolymer with poly(lactic-co-glycolic acid) (PLGA) and a chemical cross-linker; electrospinning the prepared blend; and exposing the electrospun blend to an organic solvent to remove the PLGA.
    Type: Grant
    Filed: December 20, 2013
    Date of Patent: April 10, 2018
    Assignee: University of Pittsburgh—Of the Commonwealth System of Higher Education
    Inventors: Yadong Wang, Eric M. Jeffries, Robert A. Allen
  • Patent number: 9925517
    Abstract: The present invention is related to a selective adsorption process aimed at reducing the content of nitrogen organic compounds (NOC's) that is present in hydrotreating loads (HDT) for the production of ultra-low-sulfur diesel (ULSD below 15 ppm), which is carried out at ambient temperature, atmospheric pressure, without hydrogen, using adsorbing materials with organic metal structure MIL-101-Cr-MX+ (MOF MIL-101-Cr-MX+), where MX+ can be any metal cation such as Mg2+, Al3+ or Ti4+. Likewise, the present invention considers the preparation of extrudates and the regeneration of the employed MOF adsorbent materials. In this sense, it is worth noting that the diesel HDT loads to which the present invention is referred to are hydrocarbon currents with distillation temperatures ranging from 150 to 400° C.
    Type: Grant
    Filed: May 13, 2016
    Date of Patent: March 27, 2018
    Assignee: Instituto Mexicano De Petroleo
    Inventors: Rodolfo Juventino Mora Vallejo, J. Ascencion Montoya De La Fuente, Georgina Cecilia Laredo Sanchez, Edith Meneses Ruiz, J. Jesus Castillo Munguia, Beatriz Zapata Rendon
  • Patent number: 9890176
    Abstract: The present invention relates to compounds useful for the treatment or prevention of bacteria infections. These compounds have formula I: The invention also provides processes for making the compounds described herein. Furthermore, the present invention provides a composition comprising the compounds described herein, and a pharmaceutically acceptable carrier, adjuvant, or vehicle. The present invention also provides methods of treating or preventing bacteria infection in a subject, comprising administering to the subject an effective amount of the compound or the composition described herein.
    Type: Grant
    Filed: March 12, 2014
    Date of Patent: February 13, 2018
    Assignee: Vertex Pharmaceuticals Incorporated
    Inventors: Evelyne Dietrich, Carl Poisson, Michel Gallant, Stephanie Lessard, Bingcan Liu, Sanjoy Kumar Das, Yeeman Ramtohul, Thumkunta Jagadeeswar Reddy, Julien Martel, Frederic Vallee, Jean-Francois Lévesque
  • Patent number: 9849173
    Abstract: The present disclosure provides methods for preparing vesicles. In some embodiments, the methods involve providing a molten mixture of vesicle forming lipids and then adding the molten mixture to an aqueous solution comprising an antigen such that antigen-containing vesicles are formed. In other embodiments, the methods involve providing a lyophilized lipid product and rehydrating the lyophilized lipid product with an aqueous solution comprising an antigen such that antigen-containing vesicles are formed. The lyophilized lipid product is prepared by melting vesicle-forming lipids to produce a molten lipid mixture and then lyophilizing the molten lipid mixture. The present disclosure also provides antigen-containing vesicle formulations prepared using these methods. The present disclosure also provides kits that include a lyophilized lipid product in a first container and an aqueous solution comprising an antigen in a second container.
    Type: Grant
    Filed: July 6, 2010
    Date of Patent: December 26, 2017
    Assignee: Variation Biotechnologies Inc.
    Inventors: David E. Anderson, Andrei Ogrel
  • Patent number: 9822330
    Abstract: There is provided a new material that can form a finer pattern and can be applied to adsorption/adhesion control of various cell species, proteins, viruses, and the like without the limitation of the light source. A light-degradable material comprising: a moiety that is capable of bonding to a surface of a substrate through a siloxane bond; and a structural unit of Formula (2-a) and/or Formula (2-b): (where R2 to R4 are saturated linear alkyl groups; X is a hydrogen atom or an alkyl group; Z is a carbanion or a sulfo anion; Q is an ester bond group, a phosphodiester bond group, an amido bond group, an alkylene group, or an phenylene group or a combination of these divalent groups; m1 is an integer of 1 to 200, and n is an integer of 1 to 10).
    Type: Grant
    Filed: October 11, 2013
    Date of Patent: November 21, 2017
    Assignees: NISSAN CHEMICAL INDUSTRIES, LTD., UNIVERSITY OF TOYAMA
    Inventors: Takahiro Kishioka, Shigeo Kimura, Yoshiomi Hiroi, Yuki Usui, Hiromi Kitano, Tadashi Nakaji, Makoto Gemmei
  • Patent number: 9675746
    Abstract: Extracorporeal blood treatment is performed daily on a large number of patients by use of for example a dialysis filter, plasma filter or a centrifuge. The purpose of the treatment is to separate minor components and molecules in a liquid or in the blood from larger ones, for example in connection with different disease conditions or with a view to extracting blood plasma, target substances such as blood components or molecules from for example blood donors. According to the present invention two separation steps are used together with a solution or a suspension containing at least one component, which specifically may bind to the component or the blood component to be specifically reduced, or to be refined/extracted during the treatment or during the blood treatment.
    Type: Grant
    Filed: October 18, 2010
    Date of Patent: June 13, 2017
    Assignee: GLYCOREX AB
    Inventor: Kurt G. I. Nilsson
  • Patent number: 9657132
    Abstract: The present disclosure provides amine-modified polymer foams, which may be used for wound dressing materials. For example, the modified materials can include a covalently attached molecule comprising free amine groups. Such amine groups can be used, for instance, to conjugate biologically active polypeptides and/or linkers. Methods for using modified polymers are also provided.
    Type: Grant
    Filed: October 24, 2013
    Date of Patent: May 23, 2017
    Assignee: KCI Licensing, Inc.
    Inventors: Douglas Philip Hanson, James Courage
  • Patent number: 9523109
    Abstract: The invention provides a method for preparing a peptide for improving brain function, comprising hydrolyzing milk casein with an enzymatic catalyst comprising a protease to produce a hydrolysate comprising: (i) a peptide consisting of the amino acid sequence shown in SEQ ID NO: 1 or a variant thereof; (ii) a peptide consisting of the amino acid sequence shown in SEQ ID NO: 2 or a variant thereof; or (iii) a mixture of the peptides of (i) and (ii), wherein the production yield of each of the peptides is 2% or more, or the total production yield of the mixture is 10% or more. The invention also provides a food or drink or a pharmaceutical composition containing the hydrolysate.
    Type: Grant
    Filed: June 13, 2012
    Date of Patent: December 20, 2016
    Assignee: CALPIS CO., LTD.
    Inventors: Naoto Uchida, Hiroaki Goto, Kazuhito Ohsawa, Kohji Ohki
  • Patent number: 9371563
    Abstract: The present invention relates to compositions and methods for detection and quantification of individual target molecules in biomolecular samples. In particular, the invention relates to coded, labeled probes that are capable of binding to and identifying target molecules based on the probes' label codes. Methods of making and using such probes are also provided. The probes can be used in diagnostic, prognostic, quality control and screening applications.
    Type: Grant
    Filed: March 11, 2013
    Date of Patent: June 21, 2016
    Assignees: NanoString Technologies, Inc., The Institute for Systems Biology
    Inventors: Gary K. Geiss, Sean M. Ferree, Philippa Jane Webster, Krassen M. Dimitrov
  • Patent number: 9358326
    Abstract: Substrates, optionally coated with an undercoating, having grafted thereto one or more non-fouling materials are described herein. The non-fouling, polymeric material can be grafted to a variety of functionalized substrate materials, particularly polymeric substrates and/or polymeric undercoatings immobilized on a substrate. The compositions described herein are highly resistant protein absorption, particularly in complex media and retain a high degree of non-fouling activity over long periods of time. The compositions described herein may also demonstrate antimicrobial and/or anti-thrombogenic activity. The non-fouling material can be grafted to a functionalized substrate, or optionally from an undercoating on the substrate, preferably without significantly affecting the mechanical and/or physical properties of the substrate material.
    Type: Grant
    Filed: November 8, 2012
    Date of Patent: June 7, 2016
    Assignee: ARROW INTERNATIONAL, INC.
    Inventors: Zheng Zhang, Chad Huval, William Shannan O'Shaughnessey, Michael Hencke, Trevor Squier, Jun Li, Michael Bouchard, Christopher R. Loose
  • Patent number: 9168574
    Abstract: A thixotropic gel suitable for use in subsurface bioremediation is provided along with a process of using the gel. The thixotropic gel provides a non-migrating injectable substrate that can provide below ground barrier properties. In addition, the gel components provide for a favorable environment in which certain contaminants are preferentially sequestered in the gel and subsequently remediated by either indigenous or introduced microorganisms.
    Type: Grant
    Filed: June 1, 2012
    Date of Patent: October 27, 2015
    Assignee: SAVANNAH RIVER NUCLEAR SOLUTIONS, LLC
    Inventors: Brian D. Riha, Brian B. Looney
  • Patent number: 9168680
    Abstract: One or more techniques for nano structure fabrication are provided. In an embodiment, an apparatus for manufacturing a nano structure is disclosed. The apparatus includes a stamp having a line pattern on a surface thereof that comprises a plurality of protrusions, a die configured to hold a substrate thereon, and a mechanical processing unit configured to press the plurality of protrusions of the stamp against the substrate with a predetermined pressure so as to form at least one channel pore therebetween.
    Type: Grant
    Filed: July 27, 2012
    Date of Patent: October 27, 2015
    Assignee: KOREA UNIVERSITY RESEARCH AND BUSINESS FOUNDATION
    Inventor: Kwangyeol Lee
  • Publication number: 20150140604
    Abstract: Disclosed in the present invention are an immobilized cycloaliphatic peptide acyltransferase and a preparation method and use thereof. The cycloaliphatic peptide acyltransferase is immobilized on a carrier; the cycloaliphatic peptide acyltransferase is derived from natural or artificial mutants or variants thereof, or can be obtained by introducing a foreign cyclic acyltransferase gene and transforming thereafter; the material of the carrier is selected from an inorganic carrier or a polypropylene resin carrier. Also disclosed in the present invention are the preparation method for the immobilized cycloaliphatic peptide acyltransferase and uses thereof.
    Type: Application
    Filed: May 10, 2013
    Publication date: May 21, 2015
    Inventors: Shidong Liu, Zhaoli Zhang, Xiaoming Ji
  • Patent number: 9011895
    Abstract: A method of forming and preserving a bioremodelable, biopolymer scaffold material by subjecting animal tissue to chemical and mechanical processing. In addition to skin tissue, another source of EBM is a blood vessel. EBM may be used for hernia repair, colon, rectal, vaginal and or urethral prolapse treatment; pelvic floor reconstruction; muscle flap reinforcement; lung tissue support; rotator cuff repair or replacement; periosteum replacement; dura repair; pericardial membrane repair; soft tissue augmentation; intervertebral disk repair; and periodontal repair. EBM may also be used as a urethral sling, laminectomy barrier or spinal fusion device.
    Type: Grant
    Filed: November 18, 2008
    Date of Patent: April 21, 2015
    Assignee: TEI Biosciences, Inc.
    Inventors: Jianwu Dai, Eugene Bell, Vladimir Russakovsky
  • Publication number: 20150104782
    Abstract: A method for CO2 capture may include operating a packed reactor comprising a reaction chamber containing packing including immobilized enzymes, by contacting a CO2 containing gas with a liquid solution in the reaction chamber to produce an ion-loaded solution and a CO2 depleted gas by an enzymatically catalyzed hydration reaction; monitoring enzyme activity of the immobilized enzymes; at a low enzyme activity threshold (i) stopping operation in the packed reactor, and (ii) replenishing the enzymatic activity by providing an enzyme replenishing solution into the packed reactor to contact the packing and provide a replenishing amount of the immobilized enzymes; and recommencing operation in the packed reactor for CO2 capture using the replenished immobilized enzymes. A corresponding system may include a packed reactor and an in situ enzyme supply device for supplying active enzyme within the reactor. The enzyme supply device may include spray nozzles with various configurations.
    Type: Application
    Filed: May 16, 2013
    Publication date: April 16, 2015
    Applicant: C02 Solutions Inc.
    Inventors: Eric Madore, Sylvie Fradette, Jeremy Lawson
  • Patent number: 8950068
    Abstract: A mountable device includes a bio-compatible structure embedded in a polymer that defines at least one mounting surface. The bio-compatible structure has a first side defined by a first layer of bio-compatible material, a second side defined by a second layer of bio-compatible material, an electronic component, and a conductive pattern that defines sensor electrodes. A portion of the second layer of bio-compatible material is removed by etching to create at least one opening in the second side in which the sensor electrodes are exposed. The etching further removes a portion of the first layer of bio-compatible material so as to create at least one opening in the first side that is connected to the at least opening in the second side. With this arrangement of openings, analytes can reach the sensor electrodes from either the first side or the second side of the bio-compatible structure.
    Type: Grant
    Filed: September 20, 2013
    Date of Patent: February 10, 2015
    Assignee: Google Inc.
    Inventor: James Etzkorn
  • Patent number: 8951761
    Abstract: The disclosed matter relates to immobilized enzymes and methods of use thereof.
    Type: Grant
    Filed: June 13, 2006
    Date of Patent: February 10, 2015
    Assignee: DSM Nutritional Products AG
    Inventors: Jaroslav A. Kralovec, Weijie Wang
  • Publication number: 20140377319
    Abstract: Devices and methods of altering protein concentrations in cerebrospinal fluid and/or brain interstitial fluid are disclosed. Devices include a support having at least one protease attached to the support. The device may further include a housing. Devices may be implantable for use in an in vivo active flow system or for use in an in vivo passive system. Devices may also be used in an ex vivo active flow system. Devices may also be used in a passive system to treat cerebrospinal fluid and/or brain interstitial fluid that is withdrawn, or in a passive system that is implanted surgically. Methods include contacting cerebrospinal fluid and/or brain interstitial fluid with a support including at least one protease attached to the support. Proteins contained in the cerebrospinal fluid and/or brain interstitial fluid are cleaved by the protease resulting in the reduction of protein in cerebrospinal fluid and/or brain interstitial fluid.
    Type: Application
    Filed: April 18, 2012
    Publication date: December 25, 2014
    Applicant: WASHINGTON UNIVERSITY
    Inventors: Eric C. Leuthardt, Daniel W. Moran, David Holtzman, Philip Bayly, David Brody, Ralph G. Dacey, Sam Fok, David Jeremiah Garrison, Guy M. Genin, Eriks Lusis, Rohit Pappu, Jingwei Xie, Greg Zipfel
  • Publication number: 20140370567
    Abstract: Dual-functional nonfouling surfaces and materials, methods for making dual-functional nonfouling surfaces and materials, and devices that include dual-functional nonfouling surfaces and materials. The dual-functional surfaces are nonfouling surfaces that resist non-specific protein adsorption and cell adhesion. The dual-functional surfaces and materials include covalently coupled biomolecules (e.g., target binding partners) that impart specific biological activity thereto. The surfaces and materials are useful in medical diagnostics, biomaterials and bioprocessing, tissue engineering, and drug delivery.
    Type: Application
    Filed: August 27, 2014
    Publication date: December 18, 2014
    Applicant: UNIVERSITY OF WASHINGTON
    Inventors: Shaoyi Jiang, Zheng Zhang, Shengfu Chen, Hana Vaisocherova
  • Patent number: 8911986
    Abstract: Temporary active coatings that are stabilized against inactivation by weathering are provided including a base associated with a chemically modified enzyme, and, optionally a first polyoxyethylene present in the base and independent of the enzyme. The coatings are optionally overlayered onto a substrate to form an active coating facilitating the removal of organic stains or organic material from food, insects, or the environment.
    Type: Grant
    Filed: April 29, 2011
    Date of Patent: December 16, 2014
    Assignees: Toyota Motor Engineering & Manufacturing North America, Inc., Toyota Motor Corporation
    Inventors: Hongfei Jia, Songtao Wu, Masahiko Ishii, Minjuan Zhang
  • Patent number: 8906686
    Abstract: Neo-cartilage constructs suitable for implantation into a joint cartilage lesion in situ and a method for repair and restoration of function of injured, traumatized, aged or diseased cartilage. The construct comprises at least chondrocytes incorporated into a support matrix processed according to the algorithm comprising variable hydrostatic or atmospheric pressure or non-pressure conditions, variable rate of perfusion, variable medium composition, variable temperature, variable cell density and variable time to which the chondrocytes are subjected.
    Type: Grant
    Filed: April 28, 2006
    Date of Patent: December 9, 2014
    Assignee: Histogenics Corporation
    Inventors: Shuichi Mizuno, Akihiko Kusanagi, Laurence J. B. Tarrant, Toshimasa Tokuno, Robert Lane Smith
  • Publication number: 20140356410
    Abstract: The embodiments herein provide a biodegradable and biocompatible T-plate nano-composite implant with stem cells for treating and repairing broken bones, damaged tissues and torn ligaments. The implant comprises a polymeric matrix part comprising poly lactic glycolic acids (PLGA), a bioceramic part comprising hydroxyapatite (HAp) nanoparticles and an endometrial stem cell. The PLGA and HAp nanoparticles act as a matrix and reinforcing agents respectively. A method is provided for synthesizing the T-plate implant. The method comprises synthesizing hydroxyapatite (HAp) nanoparticles, poly lactic glycolic acids (PLGA) and HAp nano composite implant. The casting of the poly lactic glycolic acids (PLGA) and HAp nano composite are done in a mold to obtain a T-plate nano composite. An endometrial stem cell from an epithelial cell lining from uterus is isolated and cultured. The endometrial stem cells are implanted on the nano-composite implant.
    Type: Application
    Filed: June 10, 2014
    Publication date: December 4, 2014
    Inventors: Jafar Ai, Nastaran Elyasi far, Mahmoud Azami, Naghmeh Bahrami, Shima Tavakol
  • Patent number: 8895046
    Abstract: Adult autologous stem cells cultured on a porous, three-dimensional tissue scaffold-implant for bone regeneration by the use of a hyaluronan and/or dexamethasone to accelerate bone healing alone or in combination with recombinant growth factors or transfected osteogenic genes. The scaffold-implant may be machined into a custom-shaped three-dimensional cell culture system for support of cell growth, reservoir for peptides, recombinant growth factors, cytokines and antineoplastic drugs in the presence of a hyaluronan and/or dexamethasone alone or in combination with growth factors or transfected osteogenic genes, to be assembled ex vivo in a tissue incubator for implantation into bone tissue.
    Type: Grant
    Filed: October 9, 2007
    Date of Patent: November 25, 2014
    Assignee: Zimmer, Inc.
    Inventors: Zou Xuenong, Haisheng Li, Cody Bunger
  • Publication number: 20140341865
    Abstract: There is provided a dressing for treating a wound. The dressing for healing a wound can be useful in maintaining a moisture environment at a wound site using a biocompatible polymer scaffold, and effectively promoting healing of a wound by various growth factors secreted by skin cells or stem cells attached to the biocompatible polymer scaffold as well.
    Type: Application
    Filed: December 12, 2012
    Publication date: November 20, 2014
    Applicant: TEGO SCIENCE INC.
    Inventors: Saewha Jeon, Yun Hee Kim, Ho Yun Chung, Jin Hyun Choi, Hankyu Jang
  • Patent number: 8883441
    Abstract: A method for detecting and counting the microorganisms in a sample is described. The method comprises: a) selectively enriching the microorganism sought in the sample, b) inducing or activating at least one enzymatic activity of the microorganism, c) immunomagnetically concentrating the microorganism, d) fluorescently labeling the microorganism, and e) detecting and analyzing the fluorescence making possible the numeration or counting of the microorganisms sought by flow cytometry, filtration cytometry or fluorescence microscopy.
    Type: Grant
    Filed: October 1, 2003
    Date of Patent: November 11, 2014
    Assignee: Metis Biotechnologies
    Inventors: Bruno Vedrine, Aline Lachaise, Vincent Carre
  • Patent number: 8877496
    Abstract: The invention provides a method for transferring cells to carriers, including: (a) providing a hydrophobic cell culture container or a cell culture container coated with a hydrophobic material on a bottom thereof; (b) adding carriers which are more hydrophilic than the hydrophobic cell culture container or hydrophobic materials and a culture medium containing cells into the hydrophobic cell culture container or the cell culture container coated with the hydrophobic material on the bottom thereof; and (c) culturing the cells, wherein the cells attach to the carriers and grow.
    Type: Grant
    Filed: June 5, 2009
    Date of Patent: November 4, 2014
    Assignee: National Taiwan University
    Inventors: Chin-Hsiung Hsieh, Yi-You Huang
  • Publication number: 20140322786
    Abstract: Provided are polymer modified substrates which comprise a) a substrate, b) a binding layer covalently attached to the surface of the substrate and covering at least a part of this surface; and c) a polymer brush formed by a plurality of polymer chains, each of which is covalently attached at one of its terminals to the binding layer. Moreover, methods are provided, for the preparation of the polymer modified substrates by polymerizing vinyl group containing monomers, such as vinylphosphonates, on a binding layer provided on a substrate.
    Type: Application
    Filed: November 13, 2012
    Publication date: October 30, 2014
    Applicant: APCETH GMBH & CO KG
    Inventors: Frank Deubel, Bernhard Rieger, Stephan Salzinger, Ning Zhang
  • Patent number: 8835144
    Abstract: Dual-functional nonfouling surfaces and materials, methods for making dual-functional nonfouling surfaces and materials, and devices that include dual-functional nonfouling surfaces and materials. The dual-functional surfaces are nonfouling surfaces that resist non-specific protein adsorption and cell adhesion. The dual-functional surfaces and materials include covalently coupled biomolecules (e.g., target binding partners) that impart specific biological activity thereto. The surfaces and materials are useful in medical diagnostics, biomaterials and bioprocessing, tissue engineering, and drug delivery.
    Type: Grant
    Filed: June 29, 2009
    Date of Patent: September 16, 2014
    Assignee: University of Washington
    Inventors: Shaoyi Jiang, Zheng Zhang, Shengfu Chen, Hana Vaisocherova
  • Patent number: 8822550
    Abstract: The present invention provides a UCST-type thermoresponsive polymer compound which responds to a temperature under physiological conditions and has biofunctionality, and various uses thereof as a thermoresponsive material. Specifically, the invention provides a thermoresponsive material containing, as an active ingredient, a polymer compound represented by Formula (I) below or an addition salt thereof, the thermoresponsive material having an upper critical solution temperature in the range of 5 to 50° C. in an aqueous solution with a salt concentration of at least 1 mM and a pH in the range of 3 to 10.5: wherein m represents an integer of 10 or more; n represents a number satisfying 0.4?n?1; R1 represents hydrogen or succinyl; and R2 represents carbamoyl.
    Type: Grant
    Filed: March 22, 2011
    Date of Patent: September 2, 2014
    Assignee: Kyushu University, National University Corporation
    Inventors: Naohiko Shimada, Atsushi Maruyama
  • Patent number: 8802410
    Abstract: There is disclosed an analytical method and a sensor suitable for carrying out the method. More specifically, there is disclosed a method for preparing a mass sensitive chemical sensor capable of detecting binding analyte species to a surface comprising cells.
    Type: Grant
    Filed: March 20, 2010
    Date of Patent: August 12, 2014
    Assignee: Attana AB
    Inventors: Camilla Käck, Julien Saint-Guirons, Björn Ingemarsson, Kerstin Lundell
  • Patent number: 8796000
    Abstract: The invention is directed to enzyme preparations which are obtainable by providing enzyme immobilizates which comprise enzymes or microorganisms comprising enzymes immobilized on an inert support with a polyethersilicone coating obtained by hydrosilylation, to a process for preparing such enzyme preparations and to the use of enzyme preparations as an industrial biocatalyst.
    Type: Grant
    Filed: July 15, 2013
    Date of Patent: August 5, 2014
    Assignee: Evonik Degussa GmbH
    Inventors: Oliver Thum, Marion Ansorge-Schumacher, Lars Wiemann, Michael Ferenz, Matthias Naumann
  • Patent number: 8796009
    Abstract: A substrate or coating is provided that includes a protease with enzymatic activity toward a component of a biological stain. Also provided is a process for facilitating the removal of a biological stain is provided wherein an inventive substrate or coating including a protease is capable of enzymatically degrading of one or more components of the biological stain to facilitate biological stain removal from the substrate or said coating.
    Type: Grant
    Filed: June 21, 2010
    Date of Patent: August 5, 2014
    Assignees: Toyota Motor Engineering & Manufacturing North America, Inc., Toyota Motor Corporation
    Inventors: Hongfei Jia, Wei Song, Masahiko Ishii, Minjuan Zhang
  • Publication number: 20140212944
    Abstract: A series of MOF-based hierarchical porous material, namely IPD-mesoMOF-1˜9, based on nanoscale MOFs of MIL-100(Al, Fe, Cr, Sc and In), MIL-53(Al), HKUST-1, DUT-5, DUT-4, MIL-101(Cr), MIL-101NDC(Cr), MIL-101BPDC(Cr) and MIL-110 respectively, forming the permanent interparticle porosities by using close (or relatively close) packing, and preparation methods thereof. Modulated or functionalized IPD-mesoMOFs can be applied for gas adsorption and molecule separation (such as CH4- and CO2-adsorption, gasoline/diesel desulfurization and purification), catalyst loadings and molecular recognition/immobilization of biological macromolecules and enzymes.
    Type: Application
    Filed: April 6, 2014
    Publication date: July 31, 2014
    Applicant: BEIJING STAR NEW MATERIAL CO., LTD.
    Inventors: Yunqi Tian, Yan Chen, Xun Liu
  • Patent number: 8778376
    Abstract: A copolymer comprising a block of an elastin pentapeptide and method of making and using the copolymer are provided.
    Type: Grant
    Filed: June 9, 2006
    Date of Patent: July 15, 2014
    Assignee: Advanced Cardiovascular Systems, Inc.
    Inventors: Syed F. A. Hossainy, Mikael Trollsas, Thierry Glauser, Yiwen Tang
  • Patent number: 8778333
    Abstract: The present invention relates to injectable compositions comprising biocompatible, hydrophilic, non-toxic and substantially spherical microspheres associated with stem cells useful for tissue construction and generation. The invention also relates to methods of tissue construction and generation, for the treatment of various tissue damage and defects, using the injectable compositions.
    Type: Grant
    Filed: January 16, 2008
    Date of Patent: July 15, 2014
    Assignee: Biosphere Medical, Inc.
    Inventors: Jean-Marie Vogel, Egisto Boschetti
  • Patent number: 8771720
    Abstract: Polyhydroxyalkanoates (PHAs) from which pyrogen has been removed are provided. PHAs which have been chemically modified to enhance physical and/or chemical properties, for targeting or to modify biodegradability or clearance by the reticuloendothelial system (RES), are described. Methods for depyrogenating PHA polymers prepared by bacterial fermentation processes are also provided, wherein pyrogens are removed from the polymers without adversely impacting the polymers' inherent chemical structures and physical properties. PHAs with advantageous processing characteristics, including low melting points and/or solubility in non-toxic solvents, are also described. The PHAs are suitable for use in in vivo applications such as in tissue coatings, stents, sutures, tubing, bone, other prostheses, bone or tissue cements, tissue regeneration devices, wound dressings, drug delivery, and for diagnostic and prophylactic uses.
    Type: Grant
    Filed: July 10, 2012
    Date of Patent: July 8, 2014
    Assignee: Metabolix, Inc.
    Inventors: Simon F. Williams, David P. Martin, Tillman Gerngross, Daniel M. Horowitz
  • Patent number: 8753856
    Abstract: The present invention provides a stable and viable biocatalyst with high activity and operational stability and method of immobilization thereof. The immobilization process is based on the principle of entrapment of partially purified enzyme precipitate which is simultaneously aggregated by cross linking agent like glutaraldehyde and further entrapped in a combination of natural polymer like gelatin and synthetic polymer like polyvinyl alcohol with effective gelation under mild conditions of temperature and pH, resulting in a stable biocatalyst. The enzymes immobilized by the above process include Penicillin acylase from rE. coli RE III (pKA18), Novel Penicillin acylase from Achromobacter sp (CCM4834) expressed in rE. coli BL21 (pK1P1) CCM 7394 and rE. coli RE III (pKX1P1).
    Type: Grant
    Filed: November 14, 2008
    Date of Patent: June 17, 2014
    Assignee: Fermenta Biotech Ltd.
    Inventors: Anupama Datla, Rajasekar Vyasarayani Williams, Trupti Krishnakant Ashar, Pavel Kyslik, Stanislav Becka
  • Patent number: 8748142
    Abstract: Materials for culturing cardiovascular tissues wherein a sponge made of a bioabsorbable material is reinforced with a reinforcement made of a bioabsorbable material.
    Type: Grant
    Filed: November 10, 2009
    Date of Patent: June 10, 2014
    Assignees: Gunze Limited, Tokyo Women's Medical University
    Inventors: Shinichiro Morita, Toshiharu Shin'Oka, Yasuharu Imai
  • Patent number: 8735117
    Abstract: In on aspect, the invention includes a microcarrier bead having a porous three-dimensional core having (a) a polymeric porous three-dimensional body having porosity of about 15 to about 90% such that at least 99% of pores are interconnected and have diameters of at most 200 microns, (b) an outer protective layer and optionally (c) a filler. In another aspect, the invention includes a method of making an artificial scaffold wherein a scaffolding material is extruded into a coolant and thereby creating a porous material having a porosity of between 15-90% such that at least 99% of pores are interconnected and have diameters of at most 200 microns.
    Type: Grant
    Filed: September 8, 2010
    Date of Patent: May 27, 2014
    Assignee: Drexel University
    Inventors: Andrew Darling, Lauren Shor, Wei Sun, Selcuk Guceri
  • Publication number: 20140142198
    Abstract: The invention provides a non-particulate cross-linked poly-?-lysine polymer. The poly-?-lysine and cross linker are linked by amide bonds and may the cross linker has at least two functional groups capable of reacting with an alpha carbon amine of poly-?-lysine. The polymer is suitably insoluble in water and other solvents and is provided in macro form for example a sheet, article or fibre. The macro form polymer is useful in a wide range of applications including wound treatment, as a medical diagnostic comprising a particulate support and a functional material bound or retained by the support and solid phase synthesis of peptides, oligonucleotides, oligosaccharides, immobilisation of species, cell culturing and in chromatographic separation.
    Type: Application
    Filed: April 20, 2012
    Publication date: May 22, 2014
    Applicant: SPHERITECH LTD
    Inventor: Donald Wellings
  • Patent number: 8729030
    Abstract: The present invention relates to peptide compounds that are agonists of the erythropoietin receptor (EPO-R). The invention also relates to therapeutic methods using such peptide compounds to treat disorders associated with insufficient or defective red blood cell production. Pharmaceutical compositions, which comprise the peptide compounds of the invention, are also provided.
    Type: Grant
    Filed: November 12, 2010
    Date of Patent: May 20, 2014
    Assignee: Affymax, Inc.
    Inventors: Christopher P. Holmes, Qun Yin, Guy Lalonde, Peter J. Schatz, David Tumelty, Palani Balu, Genet Zemede
  • Patent number: 8729235
    Abstract: The invention provides an isolated major ampullate spidroin protein, which consists of from 150 to 420 amino acid residues and is defined by the formula REP-CT. REP is a repetitive, N-terminally derived protein fragment having from 80 to 300 amino acid residues. CT is a C-terminally derived protein fragment having from 70 to 120 amino acid residues. The invention further provides an isolated fusion protein consisting of a first protein fragment, which is a major ampullate spidroin protein, and a second protein fragment comprising a fusion partner and a cleavage agent recognition site. The first protein fragment is coupled via said cleavage agent recognition site to the fusion partner. The invention also provides a method of producing a major ampullate spidroin protein and polymers thereof.
    Type: Grant
    Filed: November 21, 2013
    Date of Patent: May 20, 2014
    Assignee: Spiber Technologies AB
    Inventors: Jan Johansson, Gäran Hjäm, Margareta Stark, Anna Rising, Stefan Grip, Wilhelm Engsträm, My Hedhammar
  • Patent number: 8728796
    Abstract: Methods, processes and materials for the production and recovery of Tiacumicins produced by culturing a microorganism belonging to the species Dactylosporangium aurantiacum subspecies hamdenensis having the ability to produce and accumulate one or more Tiacumicin in a nutrient medium comprising a carbon source, a nitrogen source, trace elements such as inorganic salts, and an adsorbent, wherein said nitrogen source comprises fish powder, and wherein said Tiacumicin is produced in a yield greater than about 50 mg/L broth.
    Type: Grant
    Filed: June 12, 2012
    Date of Patent: May 20, 2014
    Assignee: Optimer Pharmaceuticals, Inc.
    Inventors: Youe-Kong Shue, Chi-Jen Frank Du, Ming-His Chiou, Mei-Chiao Wu, Yuan-Ting Chen, Franklin W. Okumu, Jonathan James Duffield
  • Publication number: 20140124454
    Abstract: A water treatment composition includes a water soluble film formed into a sealed pouch. The pouch contains a composite of a phosphate removing substance, a polymer flocculant, or an enzyme, or any combination. The phosphate removing substance, the polymer flocculant, and the enzyme are bound to each other within the composite. The pouch is added to a body of water. The pouch dissolves to release the compounds and treat the water.
    Type: Application
    Filed: November 1, 2013
    Publication date: May 8, 2014
    Applicant: HALOSOURCE, INC.
    Inventors: Everett J. Nichols, Kylie-Van H. Nguyen, James R. Scott, Martin Patrick Coles, Jeffrey F. Williams, Eric Robinson, William R. Lockett
  • Publication number: 20140127774
    Abstract: Uniform, functional polymer patches can be attached to a fraction of the surface area of living individual cells. These surface-modified cells remain viable after attachment of the functional patch. The patch does not completely occlude the cellular surface from the surrounding environment. Functional payloads carried by the patch may include, for example, drugs or other small molecules, peptides, proteins, thermally responsive polymers, and nanoparticles, or any other material that can be incorporated in a polymer patch of subcellular dimensions. The patch can include one or more polyelectrolyte multilayers (PEMs).
    Type: Application
    Filed: November 2, 2012
    Publication date: May 8, 2014
    Applicant: Massachusetts Institute of Technology
    Inventors: Albert J. Swiston, Michael F. Rubner, Robert E. Cohen, Darrell J. Irvine