Involving Esterase Patents (Class 435/19)
  • Patent number: 10421951
    Abstract: Unnatural, mutated thioesterases having an amino acid sequence that is at least 80% identical to SEQ. ID. NO: 1 and having substitutions at one or more of amino acid positions I107, R108, L109, S122, M141, E142, Y145, and L146, gene constructs encoding and configured to express the mutated thioesterases in a transformed host cell and host cells transformed to contain the gene constructs.
    Type: Grant
    Filed: June 22, 2017
    Date of Patent: September 24, 2019
    Assignees: WISCONSIN ALUMNI RESEARCH FOUNDATION, THE PENN STATE RESEARCH FOUNDATION
    Inventors: Brian F. Pfleger, Nestor Jose Hernandez-Lozada, Costas Maranas, Matthew Grisewood
  • Patent number: 10385402
    Abstract: The invention provides methods, nucleic acids and kits for detecting, or for detecting and distinguishing between or among liver cell proliferative disorders or for detecting, or for detecting and distinguishing between or among colorectal cell proliferative disorders. The invention discloses genomic sequences the methylation patterns of which have utility for the improved detection of and differentiation between said class of disorders, thereby enabling the improved diagnosis and treatment of patients.
    Type: Grant
    Filed: March 23, 2016
    Date of Patent: August 20, 2019
    Assignee: EPIGENOMICS AG
    Inventors: Catherine E. Lofton-Day, Andrew Z. Sledziewski, Ralf Lesche, Matthias Schuster, Juergen Distler, Reimo Tetzner, Thomas Hildmann, Fabian Model, Xiaoling Song
  • Patent number: 10035995
    Abstract: The invention relates to amino acid sequence variants of a lipase with improved activity for catalyzing synthesis reactions and methods of preparing the variants. The methods include predicting amino acid sites for change based on computational models of the protein structure in non-aqueous conditions, and expressing the protein in a prokaryotic host for subsequent purification and use. The enzyme sequence variants described have a three to nine-fold improvement in synthesis activity over the parent protein sequence.
    Type: Grant
    Filed: December 7, 2015
    Date of Patent: July 31, 2018
    Assignees: Eastman Chemical Company, North Carolina State University
    Inventors: Stephanie Kay Clendennen, Yaroslava Georgievna Yingling, Hoshin Kim
  • Patent number: 10000791
    Abstract: A measurement method for human pancreatic lipase activity in a sample, includes bringing a bile acid that makes a pH for giving a maximum value of human pancreatic lipase activity to be lower than 7.7, a diglyceride and a colipase into contact with the sample at pH 7.4 or lower; and detecting a signal amount varying in accordance with the human pancreatic lipase activity in the sample, and the bile acid is a bile acid containing: one of or two or more of a-type bile acids selected from the group consisting of GDCA, GCDCA, TDCA, TCDCA and salts thereof; and/or a combination of one of or two or more of b-1-type bile acids selected from the group consisting of GCA, GUDCA, TCA, TUDCA and salts thereof, and one of or two or more of b-2-type bile acids selected from the group consisting of DCA, CDCA and salts thereof.
    Type: Grant
    Filed: April 17, 2014
    Date of Patent: June 19, 2018
    Assignee: ASAHI KASEI PHARMA CORPORATION
    Inventors: Shigeru Ueda, Shinichi Sakasegawa
  • Patent number: 9951372
    Abstract: The present invention provides compounds and methods for assaying redox state of metabolically active cells and methods for assaying enzyme activity and/or metabolite level by coupling to redox defining co-factor NAD(P)/NAD(P)H measurement.
    Type: Grant
    Filed: September 20, 2013
    Date of Patent: April 24, 2018
    Assignee: PROMEGA CORPORATION
    Inventors: Wenhui Zhou, Jolanta Vidugiriene, Helene A. Benink, James J. Cali, Sarah Duellman, Dieter Klaubert, Donna Leippe, Martha O'Brien, John Shultz, Mary Sobol
  • Patent number: 9921225
    Abstract: Novel phenyl-glyoxal based anti-citrulline probes and methods of synthesis are provided. Methods of use, such as, the development of methods for monitoring substrate citrullination over time; for identifying citrullinated proteins from cells are described.
    Type: Grant
    Filed: May 20, 2016
    Date of Patent: March 20, 2018
    Assignee: The Scripps Research Institute
    Inventors: Paul R. Thompson, Kevin L. Bicker, Venkataraman Subramanian
  • Patent number: 9909155
    Abstract: Recombinant DNA techniques are used to produce oleaginous recombinant cells that produce triglyceride oils having desired fatty acid profiles and regiospecific or stereospecific profiles. Genes manipulated include those encoding stearoyl-ACP desturase, delta 12 fatty acid desaturase, acyl-ACP thioesterase, ketoacyl-ACP synthase, and lysophosphatidic acid acyltransferase. The oil produced can have enhanced oxidative or thermal stability, or can be useful as a frying oil, shortening, roll-in shortening, tempering fat, cocoa butter replacement, as a lubricant, or as a feedstock for various chemical processes. The fatty acid profile can be enriched in midchain profiles or the oil can be enriched in triglycerides of the saturated-unsaturated-saturated type.
    Type: Grant
    Filed: December 5, 2016
    Date of Patent: March 6, 2018
    Assignee: Corbion Biotech, Inc.
    Inventors: Scott Franklin, Aravind Somanchi, George Rudenko, Riyaz Bhat, Xinhua Zhao, Risha Bond, Walter Rakitsky, Alejandro Marangoni, Diza Braksmayer
  • Patent number: 9879301
    Abstract: The invention relates to a colorimetric method for detecting bacterial or fungal pathogens by detecting peptidoglycan or (1-3)-?-D-glucan in a sample.
    Type: Grant
    Filed: October 1, 2015
    Date of Patent: January 30, 2018
    Assignee: IMMUNETICS, INC.
    Inventors: Neil X. Krueger, Andrew Han, Andrew E. Levin, Victor Kovalenko
  • Patent number: 9664685
    Abstract: The invention provides a method for analyzing a protamine sample for the presence or amount of at least one nucleotidic impurity. The invention also provides a method for the quantitation of peptides in a sample of protamine comprising four major peptides and at least one related impurity.
    Type: Grant
    Filed: October 16, 2013
    Date of Patent: May 30, 2017
    Assignee: Fresenius Kabi USA, LLC
    Inventors: Jay Mowli, Pearle Torralba, Kamlesh Patel
  • Patent number: 9498489
    Abstract: A method treating cancer in a subject comprises administering to the subject a therapeutically effective amount of an antimetabolite agent that induces formation of AP sites in cancer cells of the subjects and an amount AP endonuclease inhibitor effective to potentiate the cytotoxicity of the antimetabolite agent to the cancer cells.
    Type: Grant
    Filed: April 19, 2010
    Date of Patent: November 22, 2016
    Assignee: Case Western Reserve University
    Inventors: Lili Liu, Stanton Gerson
  • Patent number: 9365889
    Abstract: Provided herein are methods for assessing the risk a test subject with heart failure has of experiencing a major adverse cardiac event, requiring revascularization, requiring a heart transplant, requiring unscheduled hospitalization for heart failure, progression of heart failure status, or any combination thereof. Also provided herein are methods for assessing the risk a test subject has of developing heart failure. The present methods comprise determining the levels of paraoxonase 1 activity in the serum, non-chelated plasma, or both in the test subject and comparing the level of PON1 activity in the test subject's sample with a control or baseline value based on levels of PON1 activity in serum, non-chelated plasma, or both samples from a population of control subjects. Also provided herein are kits useful in assessing such risks.
    Type: Grant
    Filed: January 13, 2014
    Date of Patent: June 14, 2016
    Assignee: THE CLEVELAND CLINIC FOUNDATION
    Inventor: Stanley L. Hazen
  • Patent number: 9360432
    Abstract: The present invention provides a method for a simple and accurate measurement of triglycerides in low-density lipoprotein in a sample comprising performing the following steps sequentially: (i) a step of generating free glycerol by allowing lipoprotein lipase to act on a sample, in an aqueous medium comprising the sample and a specific surfactant such as polyoxyethylene polyoxyalkylene polycyclic phenyl ether; (ii) a step of removing free glycerol present in the reaction solution of the above step (i); (iii) a step of generating free glycerol by allowing lipoprotein lipase to act on the reaction solution from which free glycerol has been removed in step (ii), in the presence of a specific surfactant such as polyoxyethylene polyoxyalkylene alkyl ether; and (iv) a step of measuring free glycerol generated in step (iii), and a kit used for the method.
    Type: Grant
    Filed: October 31, 2006
    Date of Patent: June 7, 2016
    Assignee: KYOWA MEDEX CO., LTD.
    Inventors: Yuki Katayama, Kazuhito Miyauchi, Shizuyo Takada, Tomomi Murakami
  • Patent number: 9328374
    Abstract: The present invention relates to a culture medium comprising a carbapenem, a carbapenemase activator and a M-type penicillin. It also relates to a method for detecting carbapenem-resistant bacteria in a test sample using said culture medium.
    Type: Grant
    Filed: July 5, 2012
    Date of Patent: May 3, 2016
    Assignees: Institut National de la Sante et de la Recherche Medicale (INSERM), UNIVERSITE PARIS SUD, ASSISTANCE PUBLIQUE HOPITAUX DE PARIS
    Inventors: Patrice Nordmann, Laurent Poirel, Delphine Girlich
  • Patent number: 9247694
    Abstract: The present invention is concerned with oleoresins derived from Capsicum plants producing greater than about 0.4% zeaxanthin, by weight in the dried, ripe fruit pod flesh, wherein zeaxanthin is the dominant carotenoid in the dried ripe fruit pod flesh, when measured in non-esterified forms, which plants have been developed by commercially grown Capsicum cultivars by plant breeding techniques. The oleoresin and zeaxanthin derived from these Capsicum plants can be used in applications that include nutritional supplements, foods, functional foods, cosmetics, animal feeds, aquaculture feeds, and pharmaceuticals.
    Type: Grant
    Filed: October 14, 2013
    Date of Patent: February 2, 2016
    Assignee: KALAMAZOO HOLDINGS, INC.
    Inventors: Paul H. Todd, Jr., Carrie Young, Anthony Van Den Hombergh, Donald Berdahl
  • Patent number: 9244066
    Abstract: The present application provides a system (1) that comprises a mobile phone (25) to allow testing of samples from a patient at the point of care or environmental/industrial process monitoring tests to be performed in the field. The system (1) may be easily adapted for use with a variety of different mobile phones (25). The mobile phone (25) comprises an integrated camera (15). The system (1) further comprises an optical module (20) for receiving a sample for testing. The mobile phone (25) is configured to extract the intensity and/or color information from the camera (15).
    Type: Grant
    Filed: September 9, 2011
    Date of Patent: January 26, 2016
    Assignee: DUBLIN CITY UNIVERSITY
    Inventors: Stephen O'Driscoll, Conor Burke, Brian MacCraith
  • Publication number: 20150147762
    Abstract: Pancreatic cancer can be detected using a monoclonal antibody which binds to pancreatic RNase 1 when a site in pancreatic RNase 1 capable of being modified with an N-glycan chain is not linked to a glycan chain, but which does not bind to pancreatic RNase 1 when an N-glycan chain is linked to the site. Also provided is a monoclonal antibody which can bind to pancreatic RNase 1 simultaneously with the binding of the aforementioned antibody to pancreatic RNase 1, and determining the ratio of A to B using the antibodies, wherein A represents the amount of the site located in the pancreatic RNase 1 capable of being modified with an N-glycan chain, wherein an N-glycan chain is linked or not linked to the site; and B represents the amount of the site located in the pancreatic RNase 1 capable of being modified with an N-glycan chain.
    Type: Application
    Filed: June 6, 2013
    Publication date: May 28, 2015
    Applicant: TOSOH CORPORATION
    Inventor: Daisuke Nakatani
  • Publication number: 20150140588
    Abstract: The present invention relates to variants with improved activity in an amide-bond reaction. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
    Type: Application
    Filed: July 3, 2012
    Publication date: May 21, 2015
    Applicant: Novozymes A/S
    Inventors: Werner Besenmatter, Allan Svendsen
  • Publication number: 20150126519
    Abstract: Disclosed herein are compounds and methods for inhibiting bacterial DNA repair enzymes, including AddAB and RecBCD helicase-nucleases. Pharmaceutical compositions and methods for treating a subject with an antibacterial agent are also disclosed herein.
    Type: Application
    Filed: March 20, 2013
    Publication date: May 7, 2015
    Inventors: Gerald R. Smith, Susan K. Amundsen, Ahmet C. Karabulut, Thomas D. Bannister, Reji Narayanan Nair
  • Publication number: 20150099800
    Abstract: The invention provides for novel lipase inhibitors, and compositions and devices comprising the same. The invention further provides for methods for treatment of disorders comprising administration of novel diacylglycerol lipase inhibitors, and compositions and devices comprising said inhibitors. In some embodiments, the disorders are pancreatitis, obesity, shock or pancreatic necrosis. The invention further provides for novel ether lipid reporter compounds and methods of assaying enzymatic activity comprising contacting a compound with a novel ether lipid reporter compound.
    Type: Application
    Filed: May 24, 2013
    Publication date: April 9, 2015
    Inventor: Richard I. Duclos, JR.
  • Publication number: 20150093771
    Abstract: An analytical device for analysis of chemical or biological samples, a method of using such a device, based on rotation of the device, integrated sample dosing and optical detection, and a system comprising such a device are disclosed. The analytical device comprises a device body having a liquid processing unit. The liquid processing unit comprises a mixing chamber for mixing a sample with a reagent, a sample dosing chamber for delivering a defined volume of the sample to the mixing chamber, and a reagent channel for delivering the reagent to be mixed with the sample, wherein the mixing chamber also serves as a detection chamber.
    Type: Application
    Filed: December 2, 2014
    Publication date: April 2, 2015
    Inventors: Patrick Griss, Rainer Jaeggi, Goran Savatic, Vuk Siljegovic
  • Publication number: 20150094327
    Abstract: The invention provides a fluorescence polarization (FP)-based assay to identify inhibitors of resolvase's DNA cleavage activity. The invention also provides resolvase inhibitors identified by the assay, as well as derivatives and analogs thereof. In certain embodiments, the compounds of the invention are useful to treat a poxvirus infection in an infected subject.
    Type: Application
    Filed: April 29, 2013
    Publication date: April 2, 2015
    Inventors: Frederic D. Bushman, Young Hwang, Matt Culyba
  • Publication number: 20150086998
    Abstract: Value-assigned solutions having predetermined concentrations of recombinant Lp-PLA2 are described herein. In particular, described herein are solutions of rLp-PLA2 that are stable for an extended period of time. Kits and assays include these calibration solutions, as well as methods of making and using them are described.
    Type: Application
    Filed: May 15, 2014
    Publication date: March 26, 2015
    Inventors: Thomas D. SCHAAL, Shaoqiu Zhuo
  • Publication number: 20150086999
    Abstract: Long shelf-life kits, value-assigned solutions, and methods for standardizing, verifying, calibrating or recalibrating detection of lipoprotein-associated phospholipase A2 having using them are described herein. In particular, described herein are methods of using solutions of rLp-PLA2 that are stable for an extended period of time to standardize, verify, calibrate or recalibrate assays for Lp-PLA2.
    Type: Application
    Filed: May 15, 2014
    Publication date: March 26, 2015
    Inventors: Thomas D. SCHAAL, Erhard Ralf SCHOENBRUNNER, Shaoqiu Zhuo
  • Publication number: 20150072366
    Abstract: The present invention provides methods for continuous measurement of triglyceride digestibility during a meal. A stable isotope labeled triglyceride and a free fatty acid tracer are added to the meal. The ratio between a ratio of isotope labeled fatty acid produced after digestion to free fatty acid tracer represents the percentage of digestion of triglycerides by lipase from the pancreas.
    Type: Application
    Filed: November 17, 2014
    Publication date: March 12, 2015
    Inventors: Nicolaas E. Deutz, Marielle P. Engelen, Guinur Com, John J. Thaden
  • Patent number: 8975042
    Abstract: The field of this invention relates to methods for combining genetic elements such that the activity of one of the elements provides a means for identifying, enriching, selecting for, or enhancing the activity of a second element. The invention also includes specific elements and combinations of elements.
    Type: Grant
    Filed: March 8, 2013
    Date of Patent: March 10, 2015
    Assignee: DNA Twopointo, Inc.
    Inventors: Jeremy Minshull, Elias Theodorou
  • Publication number: 20150050680
    Abstract: Providing is a new enzyme assay method for enzymes having a water-insoluble or substantially water-insoluble substrate. In the method for measuring enzymatic activity, a prescribed amount of an enzyme is disposed on a part of the surface of a gel comprising dispersoids, at least some of which are the substrate of the enzyme. Recesses formed in the surface of the gel by the action of the enzyme are measured, and the enzyme activity is calculated on the basis of the measurement results and the amount of the enzyme. The measurement of the recesses formed in the surface of the gel is performed using a method for measuring the shape and the volume of the recesses, a method for measuring changes in the optical transmittance of the gel due to the formation of the recesses, or a method for measuring changes in the optical reflectance of the gel surface due to the formation of the recesses.
    Type: Application
    Filed: February 20, 2013
    Publication date: February 19, 2015
    Applicants: Japan Agency for Marine-Earth Science and Technology, Kyokuto Pharmaeutical Industrial Co., Ltd.
    Inventors: Shigeru Deguchi, Mikiko Tsudome, Kazunori Nagaki, Nemuri Todaka, Yasunori Kurosawa
  • Publication number: 20150044715
    Abstract: Disclosed is a method which enables semiquantitative or quantitative determination of the ratio between cysteine and formylglycine residues in a protein. The method includes (a) a step of labeling the protein (i) with a halogen-substituted carboxylic acid, (ii) with a halogen-substituted carboxylic acid amide, and (iii) with a halogen-substituted carboxylic acid and then with hydrazine, or with a halogen-substituted carboxylic acid and then by oximation, (b) a step of digesting each labeled protein to provide a corresponding mixture of peptide fragments, (c) a step of subjecting each mixture to reverse phase chromatography to separate the peptide fragments from each other to produce a chromatogram, (d) a step of comparing the produced chromatograms with each other to identify the peak corresponding to the peptide fragment that contained a cysteine residue and the peak corresponding to the peptide fragment that contained a formylglycine residue.
    Type: Application
    Filed: December 18, 2012
    Publication date: February 12, 2015
    Inventor: Tetsuo Yokoyama
  • Publication number: 20150044714
    Abstract: A device and method for determining the presence or absence, or the level of, sPLA2 activity in a fluid sample. The device includes an absorbent matrix that defines a flow path for a fluid sample, a first region of the absorbent matrix for applying a fluid sample, where one of the components selected from a bioactive sPLA2 substrate and a label is dried onto or within the first region of the absorbent matrix, a second region of the absorbent matrix downstream of, and in fluid communication with, the first region for detecting an aggregated reaction product, where the other component not present in the first region is dried onto or within the second region of the absorbent matrix.
    Type: Application
    Filed: June 5, 2012
    Publication date: February 12, 2015
    Applicant: Philadelphia Health & Education Corporation d/b/a Drexel University College of Medicine
    Inventors: Timothy J. Cunningham, Katherine Marie Kollins Callaghan, Lihua Yao
  • Publication number: 20150017671
    Abstract: This invention relates to methods for determining the activity of Lp-PLA2 in at least one sample from an animal. The invention also relates to methods for determining the inhibition of Lp-PLA2 activity in samples from animals that are administered an Lp-PLA2 inhibitor.
    Type: Application
    Filed: September 26, 2014
    Publication date: January 15, 2015
    Inventors: Yaping SHOU, Yin-Fai SIU, George T. WALKER
  • Publication number: 20150010934
    Abstract: A method of screening for increased risk of fatal prostate cancer in a subject comprises providing a blood sample collected from the subject, and then detecting the presence or absence of an increased level of serum calcium in the sample. An increased level of serum calcium indicates the subject is at increased risk of fatal prostate cancer.
    Type: Application
    Filed: September 24, 2014
    Publication date: January 8, 2015
    Inventors: Gary G. Schwartz, Halcyon G. Skinner
  • Publication number: 20150008314
    Abstract: A method for detecting cancer from a biological sample previously withdrawn from the subject, in a subject includes determining in vitro the level of at least one biomarker in the biological sample. The at least one biomarker includes a phospholipid or a free fatty acid. A level of the at least one biomarker that is 2-fold greater than the level of at least one biomarker in a control is indicative of cancer in the subject.
    Type: Application
    Filed: January 25, 2013
    Publication date: January 8, 2015
    Applicant: THE CLEVELAND CLINIC FOUNDATION
    Inventors: Daniel I. Sessler, Jinbo Liu
  • Publication number: 20150005193
    Abstract: Disclosed herein are “equipment-free” flow-through assay devices based on patterned porous media, methods of making same, and methods of using same. The porous, hydrophilic media are patterned with hydrophobic barriers for performing assays on liquids.
    Type: Application
    Filed: June 20, 2014
    Publication date: January 1, 2015
    Inventors: Scott Thomas Phillips, Gregory Gerald Lewis, Jessica Sloane Robbins
  • Publication number: 20140377783
    Abstract: A novel type of dye systems comprises a selection of 10H-indolo[1,2-a]indole compounds (henceforth abbreviated as IO compounds) and (5H,7H)-indolo[1,2-a]quinoline compounds (henceforth abbreviated as IQ compounds) showing a solvatochromic effect and exhibiting strong fluorescence in a variety of materials such as polypropylene, polyethylene, oils, various solvents, emulsions. Also disclosed are various methods how the IO/IQ compounds can be administered, especially how they can be produced and administered in situ from a precursor, responding to external stimuli such as enzyme activity, temperature and so forth. The response of a precursor to external stimuli can also be used to determine the presence or absence of such stimuli.
    Type: Application
    Filed: November 4, 2011
    Publication date: December 25, 2014
    Inventors: Lukas Wick, Urs Spitz, Christophe Weymuth, Günter Schabert, Thomas Mayer, Alexander Bayer
  • Publication number: 20140370501
    Abstract: The present invention provides novel methods for determining the presence or amount of a hydrolytic enzyme in a sample, based on novel substrates for the enzymes, and also provides compositions and methods that provide highly sensitive assay methods for such hydrolytic enzymes.
    Type: Application
    Filed: June 19, 2014
    Publication date: December 18, 2014
    Inventors: Chong-Sheng YUAN, Xiaoru CHEN
  • Patent number: 8906640
    Abstract: The present invention provides methods for continuous measurement of protein and/or triglyceride digestibility during a meal. Stable isotope of 15N-spirulina protein and 2H5-phenylalanine was added to the nutritional supplement. Protein digestibility is calculated by measuring the ratio [15N]PHE to [2H5]PHE in plasma and the nutrition. In another embodiment, [1,1,1-13C3]tripalmitin and [2,2-2H2]palmitic acid were added to the meal. The ratio between [1-13C]palmitic acid/[2,2-2H2]palmitic acid will represent the percentage digestion of triglycerides by lipase from the pancreas.
    Type: Grant
    Filed: September 19, 2013
    Date of Patent: December 9, 2014
    Inventors: Nicolaas E Deutz, Marielle P Engelen, Gulnur Com, John J Thaden
  • Publication number: 20140357526
    Abstract: A method for analyzing the polypeptide content of animal tissue is described. The method includes the steps of (a) providing an animal tissue specimen; (b) depositing one or more portions of a hydrogel mixture including a protease on spatially discrete portions of the animal tissue specimen; (c) allowing sufficient time to pass for animal tissue under the hydrogel mixture to be form a digested mixture of animal tissue and hydrogel mixture; (d) removing the digested mixture from the animal tissue and extracting the polypeptides from the digested mixture to provide an extract; and (e) analyzing the polypeptide content of the extract by mass spectrometry.
    Type: Application
    Filed: June 2, 2014
    Publication date: December 4, 2014
    Applicant: Vanderbilt University
    Inventors: Richard Caprioli, Glenn A. Harris, Joshua J. Nicklay
  • Publication number: 20140349326
    Abstract: Apparatus and methods are provided for analyzing a medical condition of a user. The apparatus may include a user interface configured to receive user identification information inputted by the user, an analyzer, and a processor all disposed within a common housing. The analyzer is configured to receive a biological specimen from the user and to analyze the biological specimen to generate analysis information. The processor is configured to store and forward the analysis information and to receive prescription information. The apparatus may include a communication unit configured to transmit the user identification information and the analysis information to a doctor at a remote location for review and to receive the prescription information from the doctor. The apparatus then may dispense the prescribed medication or print a medication prescription.
    Type: Application
    Filed: February 6, 2013
    Publication date: November 27, 2014
    Inventor: Michael INGBER
  • Publication number: 20140348813
    Abstract: Provided are compositions comprising a cocaine esterase (CocE) and a compound that thermostabilizes the CocE. Also provided are methods of thermostabilizing a cocaine esterase. Additionally provided are methods of treating a mammal undergoing a cocaine-induced condition. Methods of determining whether a compound is a thermostabilizing agent for a protein are also provided. Uses of the above-described compositions for the treatment of a cocaine-induced condition is additionally provided. Additionally provided is an isolated nucleic acid encoding a CocE polypeptide having the substitutions L169K and G173Q, and the CocE polypeptide encoded by that nucleic acid, and pharmaceutical compositions thereof. Further provided is the use of that composition for the manufacture of a medicament for the treatment of a cocaine-induced condition and for the treatment of a cocaine-induced condition.
    Type: Application
    Filed: January 28, 2014
    Publication date: November 27, 2014
    Applicants: THE REGENTS OF THE UNIVERSITY OF MICHIGAN, The Trustees of Columbia University in the City of New York
    Inventors: Donald W. Landry, James H. Woods, Roger K. Sunahara, Diwahar L. Narasimhan, Joanne MacDonald, Milan N. Stojanovich, John J. Tesmer, Remy L. Brim
  • Publication number: 20140342387
    Abstract: The present invention relates to enzymes capable of hydrolysing organophosphate (OP) molecules. In particular, the invention relates to variants of the OpdA enzyme from Agrobacterium that display improved activity when compared to the naturally occurring OpdA. The invention is also towards polypeptides that have organophosphate hydrolysing activity for the organophosphates chlorpyrifos methyl, diazinon and parathion ethyl.
    Type: Application
    Filed: July 20, 2012
    Publication date: November 20, 2014
    Applicant: Commonwealth Scientific and Industrial Research Organisation
    Inventors: Colin Scott, John Oakeshott, Robyn Russell, Nigel French, Steven Kotsonis, Kaiyan Liu
  • Publication number: 20140342944
    Abstract: A kit and method for evaluating in vitro the effect of a xenobiotic (e.g., a biologic drug) on drug metabolism in hepatocytes. The kit comprises a first culture of hepatocytes, a portion of in vitro xenobiotic-stimulated biological sample, and instructions for incubating the first culture of hepatocytes with the portion of in vitro xenobiotic-stimulated biological sample, and analyzing the activity, expression, or a combination thereof of a biomarker in the hepatocytes to evaluate the effect of the xenobiotic on drug metabolism in the hepatocytes.
    Type: Application
    Filed: July 31, 2014
    Publication date: November 20, 2014
    Inventors: Maciej Czerwinski, David Benjamin Buckley, Faraz Kazmi
  • Publication number: 20140329258
    Abstract: A test strip to assist in determining the concentration of an analyte in a fluid sample comprises a base, at least one tab and a break line. The base includes a capillary channel and a test element. The capillary channel is in fluid communication with the test element. The test element is adapted to receive the fluid sample. The at least one tab is removably attached to the base. The capillary channel extends from the base into a portion of the tab. The break line intersects the capillary channel in which an inlet to the capillary channel is exposed along the break line when the tab is separated from the base.
    Type: Application
    Filed: July 15, 2014
    Publication date: November 6, 2014
    Inventor: Roger Fleming
  • Publication number: 20140329787
    Abstract: The present invention relates to a novel Phytophthora phospholipase C and uses thereof, methods of identifying modulators and inhibitors of a biological function of the phospholipase C, and methods of inhibiting Phytophthora growth comprising inhibiting a biological function of a novel Phytophthora phospholipase C.
    Type: Application
    Filed: October 17, 2012
    Publication date: November 6, 2014
    Inventors: Dean Ian Phillips, Peter Luke Beech
  • Publication number: 20140314723
    Abstract: The disclosure relates to cell culture vessels comprising fabricated cell culture surfaces providing means for efficient corneal endothelial cell growth and the use of the cultured cells in the repair of corneal tissue damage.
    Type: Application
    Filed: April 17, 2014
    Publication date: October 23, 2014
    Applicant: National University of Singapore
    Inventors: King Fai Evelyn Yim, Kim Kiat Teo, Stephanie Koo
  • Publication number: 20140308684
    Abstract: Provided herein are methods of diagnosing or monitoring the treatment of abnormal glycan accumulation or a disorder associated with abnormal glycan accumulation.
    Type: Application
    Filed: July 1, 2014
    Publication date: October 16, 2014
    Inventors: Brett E. Crawford, Jilllian R. Brown, Charles A. Glass, Jim R. Beitel, Robin M. Jackman
  • Publication number: 20140303022
    Abstract: The present invention provides novel compositions of binding moiety-nanoparticle conjugates, aggregates of these conjugates, and novel methods of using these conjugates, and aggregates. The nanoparticles in these conjugates can be magnetic metal oxides, either monodisperse or polydisperse. Binding moieties can be, e.g., oligonucleotides, polypeptides, or polysaccharides. Oligonucleotide sequences are linked to either non-polymer surface functionalized metal oxides or with functionalized polymers associated with the metal oxides. The novel compositions can be used in assays for detecting target molecules, such as nucleic acids and proteins, in vitro or as magnetic resonance (MR) contrast agents to detect target molecules in living organisms.
    Type: Application
    Filed: April 18, 2014
    Publication date: October 9, 2014
    Applicant: The General Hospital Corporation
    Inventors: Lee Josephson, Ralph Weissleder, J. Manuel Perez
  • Publication number: 20140295474
    Abstract: The invention is directed to a process for measuring the amount of digestive enzymes released from a solid composition in a dissolution medium by fluorescence spectroscopy. The invention is also directed to a combined method for measuring both the dissolution and gastroresistance of a solid compositions comprising pancrelipase.
    Type: Application
    Filed: August 8, 2012
    Publication date: October 2, 2014
    Applicant: Aptalis Pharma Ltd.
    Inventors: Massimo Latino, Luigi Ghidorsi, Giovanni Ortenzi
  • Publication number: 20140294800
    Abstract: The present invention provides, among other things, methods for the characterization of recombinant Heparan N-Sulfatase (HNS) during manufacture. The present invention uses capillary zone electrophoresis to determine the charge profile, isoform distribution, and/or glycan profile of recombinant HNS; and represents a quality feature for the batch consistency, storage stability, biological half-life, pharmacokinetic, pharmacodynamic and biological activity of the enzyme. In particular, such characterization methods may be beneficial to optimize conditions and ensure consistency for the manufacture of HNS for the treatment of a patient diagnosed with Sanfilippo syndrome using enzyme replacement therapy.
    Type: Application
    Filed: March 13, 2014
    Publication date: October 2, 2014
    Applicant: Shire Human Genetic Therapies, Inc.
    Inventor: Daniel Roseman
  • Publication number: 20140288093
    Abstract: The present disclosure provides technologies relating to lysosomal activation. The disclosure provides several strategies for increasing level and/or activity of lysosomal enzyme, and furthermore demonstrates the surprising applicability of such strategies in the treatment and/or prophylaxis of certain proteinopathies. Among other things, the present invention provides methods and compositions for the treatment and/or prophylaxis of proteinopathies other than lysosomal storage diseases through lysosomal activation. In particular, the present disclosure provides methods and compositions for the treatment and/or prophylaxis of neurodegenerative proteinopathies, and in particular those associated with accumulation of ?-synuclein. The present disclosure specifically provides methods and compositions for the treatment and/or prophylaxis of Parkinson's disease.
    Type: Application
    Filed: June 22, 2012
    Publication date: September 25, 2014
    Applicant: THE GENERAL HOSPITAL CORPORATION
    Inventors: Dmitri Krainc, Joseph R. Mazzulli
  • Publication number: 20140283157
    Abstract: The invention provides isolated anti-Lp-PLA2 antibodies that bind to Lp-PLA2. The invention also encompasses compositions comprising an anti-Lp-PLA2 antibody. These compositions can be provided in an article of manufacture or a kit. Another aspect of the invention is an isolated nucleic acid encoding an anti-Lp-PLA2 antibody, as well as an expression vector comprising the isolated nucleic acid. Also provided are cells that produce the anti-Lp-PLA2 antibodies. The invention encompasses a method of producing the anti-Lp-PLA2 antibodies. Other aspects of the invention are a method of detecting an Lp-PLA2 in a subject.
    Type: Application
    Filed: March 15, 2013
    Publication date: September 18, 2014
    Applicant: diaDexus, Inc.
    Inventors: Paul Levi Miller, Laura Corral
  • Publication number: 20140255963
    Abstract: Methods and materials relate to degradable detergents. The degradable detergents have degradable linkages that are cleaved when subjected to elevated temperature and/or reduced pressure. The detergents are compatible with spectrometric analysis, such as mass spectrometry. The surfactant comprises at least one fluorinated alkyl moiety and at least one cleavable moiety, wherein the surfactant degrades into a plurality of volatile degradation products when injected into a mass spectrometer.
    Type: Application
    Filed: June 26, 2012
    Publication date: September 11, 2014
    Inventor: Anthony Le