Involving Hydrolase Patents (Class 435/18)
  • Patent number: 10809176
    Abstract: A high-throughput optofluidic device for detecting fluorescent droplets is disclosed. The device uses time-domain encoded optofluidics to detect a high rate of droplets passing through parallel microfluidic channels. A light source modulated with a minimally correlating maximum length sequences is used to illuminate the droplets as they pass through the microfluidic device. By correlating the resulting signal with the expected pattern, each pattern formed by passing droplets can be resolved to identify individual droplets.
    Type: Grant
    Filed: October 23, 2017
    Date of Patent: October 20, 2020
    Assignee: The Trustees of the University of Pennsylvania
    Inventors: David Issadore, Venkata Yelleswarapu
  • Patent number: 10793892
    Abstract: The present invention relates to a method for detecting the presence of expanded-spectrum ß-lactamase (ß-lactamase hydrolyzing expanded-spectrum cephalosporin)-producing bacteria in a sample, said method comprising the steps of: a) performing cell lysis on a test sample in order to obtain an enzymatic suspension; b) reacting a fraction of the enzymatic suspension obtained in step a) with a reagent kit, said reagent kit comprising —expanded-spectrum ß-lactamase substrate selected from the group consisting of cephalosporins, aztreonam, and cephamycins, —a pH color indicator which will change color when the pH of the reaction mixture is comprised between 6.4 and 8.4, wherein a color change after step b) indicates the presence of expanded-spectrum ß-lactamase-producing bacteria in the test sample. The invention also relates to a reagent kit, to a microtiter plate and to their uses in detecting the presence of expanded-spectrum ß-lactamase producers in a test sample.
    Type: Grant
    Filed: November 16, 2012
    Date of Patent: October 6, 2020
    Assignees: INSERM (INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE), UNIVERSITÉ PARIS-SACLAY, ASSISTANTE PUBLIQUE HOPITAUX DE PARIS (APHP)
    Inventors: Patrice Nordmann, Laurent Dortet, Laurent Poirel
  • Patent number: 10774351
    Abstract: The present invention relates to a method of preparing an anthocyanin oligomer using a coenzyme derived from an Aspergillus sp. strain, and more particularly to a method of preparing an anthocyanin oligomer by fermenting an anthocyanin monomer with a coenzyme of Aspergillus niger, which is a kind of Aspergillus sp. strain. According to the present invention, in order to overcome contamination problems during the culturing process using Aspergillus niger, a coenzyme of Aspergillus niger is extracted and the fermentation process is performed using the same, whereby an anthocyanin oligomer characterized by reduced concern of contamination and superior radical-scavenging effects, compared to existing anthocyanin monomers, can be produced.
    Type: Grant
    Filed: July 26, 2018
    Date of Patent: September 15, 2020
    Assignee: KITTO LIFE
    Inventors: Pyo-Jam Park, Tuk-Rai Jeong, Hyun-Pil Yang, Jin Woo Hwang
  • Patent number: 10695545
    Abstract: Systems and methods for treating biologic fluids are disclosed. Some disclosed embodiments may be used to filter cerebrospinal fluid (CSF) from a human or animal subject, heat CSF to a target temperature, cool CSF to a target temperature, apply light treatment to CSF, separate cells via their dielectric properties, apply spiral and/or centrifugal separation, introduce additives to target particles, and/or apply combinations thereof. The method may include the steps of withdrawing fluid comprising CSF, treating the fluid, and returning a portion of the treated fluid to the subject. During operation of the system, various parameters may be modified, such as flow rate.
    Type: Grant
    Filed: December 2, 2016
    Date of Patent: June 30, 2020
    Assignee: MINNETRONIX, INC.
    Inventors: Blake Hedstrom, Shivanand P. Lad, Aaron McCabe, Emily Meyering, Jack Mondry, Amrita Sawhney, Elizabeth Scheurer, Matt Stoll, Abhi Vase
  • Patent number: 10689689
    Abstract: The present invention relates to methods and compositions for the stabilization of specific RNA molecules that can either be the target for detection or the control standard.
    Type: Grant
    Filed: December 20, 2016
    Date of Patent: June 23, 2020
    Assignee: Roche Molecular Systems, Inc.
    Inventor: Amar Gupta
  • Patent number: 10690597
    Abstract: The timing of the reaction of moisture-sensitive reagents for detecting the presence of analytes, e.g. leukocytes in urine samples, is used to detect when the reagents have been compromised by excess humidity. The ratio of light reflectance at wavelengths characteristic of the products of reaction between the reagents and the analyte and an infra-red reference dye is measured at two preset times after a urine sample has been applied to a test strip and used to determine whether the reagents have been compromised by excessive humidity. The presence of unusually dark samples is determined from the reflected light at 470 and 625 nm in order to confirm that the test strips are humidity-compromised.
    Type: Grant
    Filed: February 26, 2018
    Date of Patent: June 23, 2020
    Assignee: Siemens Healthcare diagnostics Inc.
    Inventors: Chris T. Zimmerle, Michael J. Pugia
  • Patent number: 10689634
    Abstract: A genetically modified organism comprising: at least one nucleic acid sequence and/or at least one recombinant nucleic acid isolated from Alicyclobacillus acidocaldarius and encoding a polypeptide involved in at least partially degrading, cleaving, transporting, metabolizing, or removing polysaccharides, cellulose, lignocellulose, hemicellulose, lignin, starch, sugars, sugar oligomers, carbohydrates, complex carbohydrates, chitin, heteroxylans, glycosides, xylan-, glucan-, galactan-, or mannan-decorating groups; and at least one nucleic acid sequence and/or at least one recombinant nucleic acid encoding a polypeptide involved in fermenting sugar molecules to a product. Additionally, enzymatic and/or proteinaceous extracts may be isolated from one or more genetically modified organisms. The extracts are utilized to convert biomass into a product.
    Type: Grant
    Filed: July 15, 2019
    Date of Patent: June 23, 2020
    Assignee: Battelle Energy Alliance LLC
    Inventors: David N. Thompson, William A. Apel, Vicki S. Thompson, Thomas E. Ward
  • Patent number: 10686017
    Abstract: A display device may include a display panel including a substrate that includes a display area and a pad area adjacent to the display area, and a first pad and a second pad on the pad area of the substrate, and a chip-on-film package over the pad area of the substrate with the first pad and the second pad in between, the chip-on-film package including an insulation layer, a first wiring on an upper surface of the insulation layer and electrically connected to the first pad, and a second wiring on a lower surface of the insulation layer and electrically connected to the second pad. A first signal having alternating voltage levels may be applied to the first wiring, and a second signal having a constant voltage level may be applied to the second wiring.
    Type: Grant
    Filed: January 28, 2019
    Date of Patent: June 16, 2020
    Assignee: Samsung Display Co., Ltd.
    Inventors: Cheolhwan Eom, Kwang-Min Kim, Hyeaweon Shin, Sang Joon Ryu, Hyungjun An, Minji Lee, Yul Kyu Lee, Jeahyun Lee
  • Patent number: 10662453
    Abstract: Methods that may be used for the manufacture of the chemical compound (S)-norcoclaurine, (S)-norlaudanosoline, and (S)-norcoclaurine or (S)-norlaudanosoline synthesis intermediates are provided. (S)-Norcoclaurine, (S)-norlaudanosoline, and (S)-norcoclaurine or (S)-norlaudanosoline synthesis intermediates are useful as precursor products in the manufacture of certain medicinal agents.
    Type: Grant
    Filed: September 5, 2018
    Date of Patent: May 26, 2020
    Assignee: Willow BioSciences Inc.
    Inventor: Peter James Facchini
  • Patent number: 10640743
    Abstract: A thin film culture device for detecting yeast and mold microorganisms in a sample is provided. The culture device comprises a body comprising a self-supporting substrate having a first major surface and a second major surface; a first adhesive composition disposed on a portion of the first major surface of the substrate; a substantially dry, cold-water-soluble first hydrogel-forming composition adhered to the first adhesive composition; and a plurality of indicator agents. The plurality of indicator agents comprises three indicator agents for detecting distinct glycosidase enzyme activities, an indicator agent for detecting an alkyl esterase enzyme activity, and an indicator agent for detecting a phosphatase enzyme activity, wherein each of the plurality of indicator agents comprises a detectable reporter group. A method of using the culture device is also provided.
    Type: Grant
    Filed: January 25, 2018
    Date of Patent: May 5, 2020
    Assignee: 3M Innovative Properties Company
    Inventors: Sailaja Chandrapati, Tera M. Nordby
  • Patent number: 10597695
    Abstract: The invention relates to a mutant polypeptide having creatine amidinohydrolase activity, where the polypeptide both retains thermostable activity in the presence of reagents that modify thiol groups. The invention further relates to methods for producing the mutant polypeptide; and to a sensor comprising the mutant polypeptide for use in the measurement of creatinine in samples of physiological fluids. Additionally, the invention teaches how to use the mutant polypeptide to enhance the life-time of a creatinine sensor, and a method for producing the sensor.
    Type: Grant
    Filed: February 23, 2016
    Date of Patent: March 24, 2020
    Assignee: RADIOMETER MEDICAL APS
    Inventors: Palle Schneider, Jens Østergaard, Thomas Steen Hansen
  • Patent number: 10532195
    Abstract: Systems and methods for treating biologic fluids are disclosed. Some disclosed embodiments may be used to filter cerebrospinal fluid (CSF) from a human or animal subject, heat CSF to a target temperature, cool CSF to a target temperature, apply light treatment to CSF, separate cells via their dielectric properties, apply spiral and/or centrifugal separation, introduce additives to target particles, and/or apply combinations thereof. The method may include the steps of withdrawing fluid comprising CSF, treating the fluid, and returning a portion of the treated fluid to the subject. During operation of the system, various parameters may be modified, such as flow rate.
    Type: Grant
    Filed: December 2, 2016
    Date of Patent: January 14, 2020
    Assignee: MINNETRONIX, INC.
    Inventors: Blake Hedstrom, Shivanand P. Lad, Aaron McCabe, Emily Meyering, Jack Mondry, Amrita Sawhney, Elizabeth Scheurer, Matt Stoll, Abhi Vase
  • Patent number: 10501548
    Abstract: There is disclosed an assay method for selectively detecting 1,25-dihydroxy-vitamin D in a biological fluid sample. According to the method, the pH of the test sample is adjusted to 6-9 and a receptor protein comprising the Ligand Binding Domain of Vitamin D Receptor (VDR-LBD) is added to the test sample, thereby obtaining the formation of a VDR-LBD/1,25-dihydroxyvitamin D complex in which the VDR-LBD portion is conformationally changed with respect to unbound VDR-LBD. The VDR-LBD/1,25-dihydroxyvitamin D complex is then detected by means of a capture moiety which is capable of specifically binding to VDR-LBD bound to 1,25-dihydroxyvitamin D. Also disclosed are an assay kit and an antibody for carrying out the method. The assay is preferably a sandwich immunoassay.
    Type: Grant
    Filed: May 26, 2017
    Date of Patent: December 10, 2019
    Assignee: DiaSorin S.p.A.
    Inventors: Joshua Soldo, Gregory Olson, Michael Lutterman, John Wall, Michael New, Hector Floyd Deluca, Fabrizio Bonelli
  • Patent number: 10494659
    Abstract: Provided is a process for producing a substrate solution for measuring lipase activity including, as a substrate for measuring lipase activity, 1,2-o-dilauryl-rac-glycero-3-glutaric acid (6?-methylresorufin) ester, wherein the production process does not necessitate cumbersome or special processing such that skill is required, or does not necessitate a special apparatus, instruments, or other items. This production process is a process for producing a substrate solution for measuring lipase activity, and is characterized by including the steps of (1) mixing the substrate for measuring lipase activity and a side-chain-type nonreactive polyether-modified-type modified silicone oil or a polyoxyethylene/polyoxypropylene condensate to prepare a mixture, and (2) mixing all or a portion of the mixture of step (1) with water or an aqueous solution.
    Type: Grant
    Filed: February 10, 2017
    Date of Patent: December 3, 2019
    Assignee: Shino-Test Corporation
    Inventors: Naomi Iizuka, Atsushi Hikichi
  • Patent number: 10488363
    Abstract: In an embodiment, a system for measuring soil element concentration in a field in real time is disclosed. The system comprises an extraction apparatus coupled to a mobility component configured to move the system in the agricultural field. The extraction apparatus configured to receive a plurality of soil samples successively from a soil probe coupled to the mobility component, when the mobility component is operating. The extraction apparatus containing an extractant solution that is a solvent of the soil samples. In addition, the extraction apparatus comprising a mixer that is configured to mix the soil samples with the extractant solution, thereby forming a solution mix. The system also comprises a chemical sensor coupled to the extraction apparatus, the chemical sensor configured to measure a concentration level of a soil element in the solution mix.
    Type: Grant
    Filed: October 3, 2017
    Date of Patent: November 26, 2019
    Assignee: The Climate Corporation
    Inventors: Miao Liu, Luis Jurado
  • Patent number: 10466260
    Abstract: Provided herein are compositions, systems, and methods for extracting and detecting at least one HDL-associated protein (e.g., ApoA1) from a sample (e.g., plasma or serum sample). In certain embodiments, a strong organic acid and hydrophilic organic solvent are mixed with the sample; after centrifugation, the supernatant is transferred to a second container where it is mixed with a non-polar organic solvent; after centrifugation, the lower aqueous layer is transferred to a third container; and then at least a portion of the transferred aqueous layer is subjected to a detection assay such that at least one HDL-associated protein is detected.
    Type: Grant
    Filed: October 20, 2017
    Date of Patent: November 5, 2019
    Assignee: Cleveland Heartlab, Inc.
    Inventors: Cory Bystrom, Timothy Collier
  • Patent number: 10451624
    Abstract: A reagent kit used for detecting lipoprotein-associated phospholipase A2, and a preparation method for the reagent kit. The reagent kit comprises one or a plurality of first anti-lipoprotein-associated phospholipase A2 antibodies used for binding lipoprotein-associated phospholipase A2 to be measured, and one or a plurality of second anti-lipoprotein-associated phospholipase A2 antibodies marked with a trace marker and binding with the lipoprotein-associated phospholipase A2 to be measured at another site, different from the binding site of the lipoprotein-associated phospholipase A2 to be measured and the first anti-lipoprotein-associated phospholipase A2 antibodies. The reagent kit also comprises a displacing agent, so as to further increase the detection accuracy of the reagent kit.
    Type: Grant
    Filed: February 10, 2015
    Date of Patent: October 22, 2019
    Assignee: SHENZHEN NEW INDUSTRIES BIOMEDICAL ENGINEERING CO., LTD
    Inventors: Wei Rao, Jinyun Yuan, Qin Li, Ke Huang, Kai Luo, Dongxia Lin, Tinghua Li
  • Patent number: 10444149
    Abstract: A method may comprise: exposing a substituted chromone dissolved in a solvent to a sample; taking a fluorescence measurement of the sample after exposure to the substituted chromone; and determining a presence or absence of one or more ions in the sample, a concentration of the one or more ions in the sample, or both based on the fluorescence measurement.
    Type: Grant
    Filed: February 23, 2017
    Date of Patent: October 15, 2019
    Assignee: Ohio Northern University
    Inventor: Jake R. Zimmerman
  • Patent number: 10428340
    Abstract: This invention relates to phytases, polynucleotides encoding them, uses of the polynucleotides and polypeptides of the invention, as well as the production and isolation of such polynucleotides and polypeptides. In particular, the invention provides polypeptides having phytase activity under high temperature conditions, and phytases that retain activity after exposure to high temperatures. The invention further provides phytases which have increased gastric lability. The phytases of the invention can be used in foodstuffs to improve the feeding value of phytate rich ingredients. The phytases of the invention can be formulated as foods or feeds or supplements for either to, e.g., aid in the digestion of phytate. The foods or feeds of the invention can be in the form of pellets, liquids, powders and the like.
    Type: Grant
    Filed: April 23, 2018
    Date of Patent: October 1, 2019
    Assignee: Syngenta Participations AG
    Inventors: David P. Weiner, Ame I. Solbak, Jr., Ryan McCann
  • Patent number: 10401361
    Abstract: This invention relates to substrates and methods for the visualization of intracellular organelles, such as the lysosome, peroxiosome, nucleus, Endoplasmic Reticulum and Golgi Apparatus, based upon organelle enzyme activity. Such compounds represent a novel combination of chemically distinct enzyme substrates with targeting and detection substrates which are activated by enzyme activity inside target organelles to produce a detectable signal. The organelle targeted enzyme substrates of this invention are designed to provide high fluorescence at lower pH values found in some organelles and can be used for monitoring enzyme activity inside cells at very low concentrations.
    Type: Grant
    Filed: December 11, 2017
    Date of Patent: September 3, 2019
    Assignee: Marker Gene Technologies, Inc.
    Inventors: John Joseph Naleway, Fiona Karen Harlan, Jason Scott Lusk
  • Patent number: 10385326
    Abstract: The present invention relates to GH Family 11 xylanase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
    Type: Grant
    Filed: May 22, 2015
    Date of Patent: August 20, 2019
    Assignee: NOVOZYMES A/S
    Inventors: James Lavigne, Brian R. Scott, Daniel Sebastian Kolczynski
  • Patent number: 10364445
    Abstract: The invention relates to processes of producing a fermentation product, comprising liquefying a starch containing material with an alpha-amylase; pre-saccharifying and/or saccharifying and fermenting using a fermentation organism in the presence of a carbohydrate source generating enzyme and a cellulolytic composition The invention also relates to methods of dewatering whole stillage.
    Type: Grant
    Filed: November 27, 2017
    Date of Patent: July 30, 2019
    Assignee: NOVOZYMES A/S
    Inventors: Thomas Rasmussen, Jeremy Saunders, James Croonenberghs, Zhengfang Kang, Joyce Craig, Michael John Akerman
  • Patent number: 10365268
    Abstract: The present invention provides methods to modulate key elements along the DAG signaling pathway as well as a diagnostic assay, device and methods of using the same to diagnose bipolar disorder (BD) and attention deficit hyperactivity disorder (ADHD). Methods to identify diagnostic markers and drug targets for BD and ADHD. Methods of identifying effective compounds responsible for membrane potentials and excitabilities influencing bipolar disorder (BD) and attention deficit hyperactivity disorder (ADHD). Methods of identifying an effective compound that modulates the activity of Ca2+/CaM enzyme and compounds involved in changing the K+ gradient across the plasma membrane thereby increasing or decreasing the membrane potential ratio (MPR™) values. The invention provides methods of identifying a compound that modulates the activity of PKC which is an important protein of the DAG signaling pathway.
    Type: Grant
    Filed: September 26, 2016
    Date of Patent: July 30, 2019
    Assignee: PsychNostics, LLC
    Inventor: Alagu P. Thiruvengadam
  • Patent number: 10351835
    Abstract: A genetically modified organism comprising: at least one nucleic acid sequence and/or at least one recombinant nucleic acid isolated from Alicyclobacillus acidocaldarius and encoding a polypeptide involved in at least partially degrading, cleaving, transporting, metabolizing, or removing polysaccharides, cellulose, lignocellulose, hemicellulose, lignin, starch, sugars, sugar oligomers, carbohydrates, complex carbohydrates, chitin, heteroxylans, glycosides, xylan-, glucan-, galactan-, or mannan-decorating groups; and at least one nucleic acid sequence and/or at least one recombinant nucleic acid encoding a polypeptide involved in fermenting sugar molecules to a product. Additionally, enzymatic and/or proteinaceous extracts may be isolated from one or more genetically modified organisms. The extracts are utilized to convert biomass into a product.
    Type: Grant
    Filed: August 14, 2017
    Date of Patent: July 16, 2019
    Assignee: Battelle Energy Alliance, LLC
    Inventors: David N. Thompson, William A. Apel, Vicki S. Thompson, Thomas E. Ward
  • Patent number: 10344061
    Abstract: The present invention relates to peptides exhibiting hair growth promoting activity and/or melanin production promoting activity, a composition for ameliorating hair loss comprising one or both of the peptides as an active ingredient, a composition for promoting hair growth and/or hair restoration comprising one or both of the peptides as an active ingredient, a use of one or both of the peptides for ameliorating hair loss, a use of one or both of the peptides for promoting hair growth and/or hair restoration, a pharmaceutical composition for the treatment of melanin hypopigmentation comprising one or both of the peptides as an active ingredient, a cosmetic composition for the treatment of melanin hypopigmentation comprising one or both of the peptides as an active ingredient, and a use of one or both of the peptides for the amelioration and/or treatment of melanin hypopigmentation.
    Type: Grant
    Filed: February 24, 2017
    Date of Patent: July 9, 2019
    Assignee: CAREGEN CO., LTD.
    Inventors: Yong Ji Chung, Eun Mi Kim, Eung Ji Lee
  • Patent number: 10344050
    Abstract: The present invention relates to an in vitro method for production of heterodimeric proteins.
    Type: Grant
    Filed: October 26, 2012
    Date of Patent: July 9, 2019
    Assignee: GENMAB A/S
    Inventors: Michael Gramer, Amitava Kundu, Ewald T. J. Van Den Bremer, Muriel Van Kampen, Patrick Priem, Aran Frank Labrijn, Joyce I. Meesters, Joost J. Neijssen, Janine Schuurman, Paul Parren, Patrick Van Berkel, Werner L. Vos, Arnout Gerritsen
  • Patent number: 10316323
    Abstract: Disclosed are genetically engineered organisms, such as yeast and bacteria, that have the ability to metabolize atypical nitrogen sources, such as melamine and cyanamide. Fermentation methods using the genetically engineered organisms are also described. The methods of the invention are robust processes for the industrial bioproduction of a variety of compounds, including commodities, fine chemicals, and pharmaceuticals.
    Type: Grant
    Filed: August 17, 2017
    Date of Patent: June 11, 2019
    Assignee: Novogy, Inc.
    Inventors: Colin South, Arthur J Shaw, IV
  • Patent number: 10253299
    Abstract: The present invention relates to a sulfated cellulose hydrate membrane, a method for the preparation thereof and the use of the membrane as adsorption membrane for the purification of viruses.
    Type: Grant
    Filed: September 11, 2014
    Date of Patent: April 9, 2019
    Assignee: Sartorius Stedium Biotech GMBH
    Inventors: Louis Villain, Hans-Heinrich Hörl, Christian Brumm
  • Patent number: 10232374
    Abstract: Methods are provided for the preparation of a sample using a digital microfluidic platform and the optional subsequent mass analysis of an extracted analyte. A sample is dried, optionally on a solid phase support, and contacted with digital microfluidic array. An analyte present within the dried sample is extracted into an extraction solvent by electrically addressing the digital microfluidic array to transport a droplet of extraction solvent to the dried sample spot. The extracted sample may be dried and subsequently processed on the digital microfluidic array for derivatization. The digital microfluidic device may further include an integrated microfluidic channel having an output aperture, and the method may further include contacting a droplet containing extracted analyte with the microfluidic channel and applying a suitable electric field for generating nano-electrospray, thereby enabling the device to be directly interfaced with a mass analysis device.
    Type: Grant
    Filed: April 15, 2011
    Date of Patent: March 19, 2019
    Assignee: mirOculus Inc.
    Inventors: Mais J. Jebrail, Hao Yang, Aaron R. Wheeler
  • Patent number: 10196449
    Abstract: There is disclosed an assay method for selectively detecting 1,25-dihydroxy-vitamin D in a biological fluid sample. According to the method of the invention, the pH of the test sample is adjusted to 6-9 and a receptor protein comprising the Ligand Binding Domain of Vitamin D Receptor (VDR-LBD) is added to the test sample, thereby obtaining the formation of a VDR-LBD/1,25-dihydroxyvitamin D complex in which the VDR-LBD portion is conformationally changed with respect to unbound VDR-LBD. The VDR-LBD/1,25-dihydroxyvitamin D complex is then detected by means of a capture moiety which is capable of specifically binding to VDR-LBD bound to 1,25-dihydroxyvitamin D. Also disclosed are an assay kit and an antibody for carrying out the method of the invention. The assay of the invention is preferably a sandwich immunoassay.
    Type: Grant
    Filed: January 27, 2014
    Date of Patent: February 5, 2019
    Assignee: DiaSorin S.p.A.
    Inventors: Joshua Soldo, Gregory Olson, Michael Lutterman, John Wall, Michael New, Hector Floyd Deluca, Fabrizio Bonelli
  • Patent number: 10154997
    Abstract: Methods of using KDAC inhibitor compounds for the treatment of various parasitic diseases are described.
    Type: Grant
    Filed: August 4, 2016
    Date of Patent: December 18, 2018
    Assignee: Washington University
    Inventors: Garland R. Marshall, Makedonka Mitreva
  • Patent number: 10144919
    Abstract: The present application provides mutants of wild type phospholipase C (PLC) specific to phosphatidylcholine from Bacillus cereus. The related mutations include mutation of asparagine at position 63 to another amino acid, also including mutation of arginine at position 20 to histidine and of alanine at position 83 to aspartic acid. The present application also provides a nucleic acid molecule encoding the mutant, a vector containing the nucleic acid molecule, and a cell containing the nucleic acid molecule or vector. The present application also provides uses of the mutant, nucleic acid molecule vector, and cell.
    Type: Grant
    Filed: November 4, 2014
    Date of Patent: December 4, 2018
    Assignee: Wilmar (Shanghai) Biotechnology Research & Development Center Co., LTD
    Inventors: Jun Xu, Yaoji Xuan, Jinmin Li
  • Patent number: 10132812
    Abstract: To provide means for reducing reaction interference observed when AT III is subjected to limulus test, whereby the limulus test of antithrombin III can be carried out at high accuracy. Reaction interference observed when AT III is subjected to limulus test can be reduced by subjecting AT III to a protein inactivation treatment in the co-presence of a divalent metal salt.
    Type: Grant
    Filed: December 27, 2013
    Date of Patent: November 20, 2018
    Assignee: SEIKAGAKU CORPORATION
    Inventors: Maki Aizawa, Toshio Oda, Jun Aketagawa
  • Patent number: 10072286
    Abstract: The present invention relates to a reference substance and a nucleic acid chip for generating binding information on biomolecules and analysis single-stranded nucleic acids in a biosample composed of biomolecules; a method for preparing the same; and a method for analyzing biomolecules using the same, and the reference substance and the nucleic acid chip can be used for analyzing the biological significance of the biomolecules. In addition, the present invention relates to a method for preparing an external reference substance and a biochip for generating the binding information on biomolecules and ligands; and a method for analyzing biomolecules using the same. The external reference substance and the biochip of the present invention can be used in the field of analyzing the biological significance of the biomolecules.
    Type: Grant
    Filed: May 8, 2014
    Date of Patent: September 11, 2018
    Inventor: Sung-Chun Kim
  • Patent number: 10060928
    Abstract: Methods for determining the endotoxin content of an aluminum salt preparation for use in medicine are provided. The methods include mixing the aluminum salt with a desorption buffer and separating the aluminum salt from the endotoxin.
    Type: Grant
    Filed: June 5, 2014
    Date of Patent: August 28, 2018
    Assignee: GlaxoSmithKline Biologicals, S.A.
    Inventor: Clementine Mascaux
  • Patent number: 10047367
    Abstract: Provided are vector constructs and methods for expressing a transgene in plant cells and/or plant tissues using gene regulatory elements, including the promoters, 5?-UTRs, and/or 3?-UTRs, isolated from Zea mays.
    Type: Grant
    Filed: October 15, 2014
    Date of Patent: August 14, 2018
    Assignee: Dow AgroSciences LLC
    Inventors: Manju Gupta, Navin Elango, Karthik N. Muthuraman, Jeffrey Beringer, Sara Bennett, Huixia Wu, Shavell Gorman, Andrew Worden
  • Patent number: 10047368
    Abstract: Provided are vector constructs and methods for expressing a transgene in plant cells and/or plant tissues using gene regulatory elements, including the promoters, 5?-UTRs, introns, and/or 3?-UTRs, isolated from Zea mays.
    Type: Grant
    Filed: October 15, 2014
    Date of Patent: August 14, 2018
    Assignee: Dow AgroSciences LLC
    Inventors: Manju Gupta, Navin Elango, Karthik N. Muthuraman, Jeffrey Beringer, Sara Bennett, Huixia Wu
  • Patent number: 10048257
    Abstract: The present invention relates to microspheres comprising protein signal precursor molecules, or a carrier protein bonded to signal precursor molecules, wherein said signal precursor molecules are activatable to generate a detectable signal while remaining bonded to the carrier protein. Also disclosed is a method of making such microspheres comprising the steps of mixing protein molecules with a matrix former in solution; adding a reducing reagent to the mixture; removing the reducing reagent; and removing the matrix former to leave microspheres of protein molecules. Also disclosed are bioassay methods using the microspheres to provide signal amplification, including an amplification cycling procedure.
    Type: Grant
    Filed: August 27, 2015
    Date of Patent: August 14, 2018
    Assignee: Supernova Diagnostics, Inc.
    Inventors: Wing Cheung Mak, Ling Wai Wong, Pui Yee Cangel Chan, Reinhard Renneberg
  • Patent number: 10010616
    Abstract: The invention described herein pertains to compounds and conjugates, to compositions, complexes and formulations comprising the compounds and/or conjugates, and to methods of use of the compounds, conjugates and their compositions, complexes and formulations in vaccines and vaccinations and generating immune responses.
    Type: Grant
    Filed: January 27, 2012
    Date of Patent: July 3, 2018
    Assignee: PURDUE RESEARCH FOUNDATION
    Inventors: Richard Frederic Borch, Irene A. George, Harm Hogenesch, Stanley L. Hem, Lawrence E. Hem
  • Patent number: 10012594
    Abstract: Disclosed are methods, apparatuses, and genetically modified bacteria that may be used to detect bacteriophages in a sample. In some embodiments, a rapid detection test is disclosed to test for the presence of coliphages which may indicate the presence of human or animal waste contamination in water samples.
    Type: Grant
    Filed: November 2, 2015
    Date of Patent: July 3, 2018
    Inventors: James K. Campbell, Lyuda Trokhina
  • Patent number: 9969991
    Abstract: Provided herein are substantially non-luminescent peptide/polypeptide tags that are inserted internally within a protein of interest or between N-terminal and C-terminal peptides/polypeptides. Interaction of the internally-inserted tag with a complement polypeptide/peptide that is also substantially non-luminescent results in the formation a bioluminescent reporter complex.
    Type: Grant
    Filed: September 11, 2015
    Date of Patent: May 15, 2018
    Assignee: Promega Corporation
    Inventors: Andrew S. Dixon, Lance P. Encell, Thomas Machleidt, Marie Schwinn, Keith Wood, Monika Wood, Kris Zimmerman
  • Patent number: 9969737
    Abstract: Chromogenic or fluorescent carbapenems according to formula I, wherein Ar is a mono or disubstituted carbocyclic aromatic group or an optionally mono or disubstituted heterocyclic aromatic group, are useful compounds for the detection of bacterial carbapenemase.
    Type: Grant
    Filed: February 29, 2016
    Date of Patent: May 15, 2018
    Assignee: Hans Rudolf Pfaendler
    Inventor: Hans R. Pfaendler
  • Patent number: 9970050
    Abstract: Methods and compositions for the detection and quantification of nucleic acids are provided. In one embodiment, a sample is contacted with a primer complementary to a first region of a target nucleic acid and a probe complementary to a second region of the target nucleic acid downstream of the first region under conditions suitable for hybridization of the target nucleic acid with the primer and the probe. The probe in this embodiment comprises a fluorophore and is attached to a solid support. The hybridized probe is cleaved with a nucleic acid polymerase having exonuclease activity to release the reporter from the solid support. The presence of the target nucleic acid is then detected and optionally quantified by detecting a decrease in signal from the reporter on the solid support.
    Type: Grant
    Filed: January 22, 2015
    Date of Patent: May 15, 2018
    Assignee: LUMINEX CORPORATION
    Inventors: Brian Schrader, Douglas F. Whitman
  • Patent number: 9932591
    Abstract: The invention relates a method for producing a stable recombinant protein, comprising growing a non-naturally occurring host cell in a culture medium to produce a recombinant protein, and making a composition comprising the recombinant protein and a polysorbate. The production of endogenous lipoprotein lipase by the host cell is reduced. The endogenous lipoprotein lipase is present in the composition in a small amount, and is capable of degrading the polysorbate. The invention also relates to the relevant host cells and compositions, and preparation thereof.
    Type: Grant
    Filed: December 18, 2014
    Date of Patent: April 3, 2018
    Assignee: University of Delaware
    Inventors: Kelvin Lee, Abraham Lenhoff, Kristin Valente, Nick Levy, Yatin Gokarn
  • Patent number: 9885659
    Abstract: Method and apparatus for determining a quality or characteristic of connectors in electronic components is provided. Methods include applying a UV-responsive indicator solution active for Pd, Ni, or Cu to a connector on an electrical component; irradiating the connector with UV radiation; detecting a response to the UV radiation; and determining a quality of the connector based on the response to the UV radiation. Apparatus includes an enclosure; a support; a dispenser oriented toward the substrate support; a source of UV-responsive indicator solution active for Pd, Ni, or Cu ions fluidly coupled to the dispenser; a UV source coupled to the enclosure; and a radiation sensor positioned to detect light inside the enclosure.
    Type: Grant
    Filed: October 4, 2016
    Date of Patent: February 6, 2018
    Assignee: INTERNATIONAL BUSINESS MACHINES CORPORATION
    Inventors: Eric J. Campbell, Sarah K. Czaplewski, Joseph Kuczynski, Timothy J. Tofil
  • Patent number: 9879303
    Abstract: Chromogenic substrates for ?-D-glucuronidase activity comprising monoglucuronides of some 1,2-dihydroxyaromatic derivatives. When cleaved these form soluble colored conjugates with multivalent metal ions such as iron ions. The substrates may be used in conjunction with chromogenic substrates for other enzymes in microbial detection and identification especially involving liquid media. Microbes can be grown in the presence of the substrates and the compounds providing the metal ion. The substrates are particularly useful for detecting ?-D-glucuronidase-positive E. coli. Synthetic methods for making the compounds are described.
    Type: Grant
    Filed: November 3, 2014
    Date of Patent: January 30, 2018
    Assignee: GLYCOSYNTH LIMITED
    Inventors: Hayley Jane Turner, Michael Burton
  • Patent number: 9867927
    Abstract: A carrier for blood component adsorption enables selective and efficient adsorption removal of granulocytes and monocytes and, at the same time, enables adsorption removal of inflammatory cytokines. The carrier for blood component adsorption includes a water-insoluble carrier having a surface to which a functional group(s) containing a silyl group and an amino group is/are introduced.
    Type: Grant
    Filed: February 24, 2012
    Date of Patent: January 16, 2018
    Assignee: Toray Industries, Inc.
    Inventors: Naotoshi Tomita, Yoshiyuki Ueno, Kazuhiro Tanahashi
  • Patent number: 9841426
    Abstract: This invention relates to substrates and methods for the visualization of intracellular organelles, such as the lysosome, peroxiosome, nucleus, Endoplasmic Reticulum and Golgi Apparatus, based upon organelle enzyme activity. Such compounds represent a novel combination of chemically distinct enzyme substrates with targeting and detection substrates which are activated by enzyme activity inside target organelles to produce a detectable signal. The organelle targeted enzyme substrates of this invention are designed to provide high fluorescence at lower pH values found in some organelles and can be used for monitoring enzyme activity inside cells at very low concentrations.
    Type: Grant
    Filed: April 17, 2015
    Date of Patent: December 12, 2017
    Assignee: Marker Gene Technologies, Inc
    Inventors: John Joseph Naleway, Fiona Karen Harlan, Jason Scott Lusk
  • Patent number: 9829481
    Abstract: The instant invention relates to novel compounds useful for visualizing cell senescence, their preparation and use. In particular, this invention relates to novel fucose and amino-quinoline derivatives useful as senescence traces and their preparation.
    Type: Grant
    Filed: February 26, 2015
    Date of Patent: November 28, 2017
    Assignee: EBERHARD KARLS UNIVERSITAET TUEBINGEN MEDIZINISCHE FAKULTAET
    Inventors: Jonathan Cotton, Bernd Pichler, Kerstin Fuchs, Anna Teske, Marcel Krueger, Christian Kesenheimer, Klaus Schulze-Osthoff, Dominic Hildebrand
  • Patent number: 9816097
    Abstract: Nucleic acid promoters isolated from Panicum virgatum capable of transcriptional activation of heterologous nucleic acids are provided. Constructs, vectors and transgenic plants that include nucleic acid promoters are described. Methods for producing heterologous proteins in transgenic plants by transforming the plants with vectors and constructs are also provided.
    Type: Grant
    Filed: May 29, 2013
    Date of Patent: November 14, 2017
    Assignee: AGRIVIDA, INC.
    Inventors: R. Michael Raab, Oleg Bougri