Involving Phosphatase Patents (Class 435/21)
  • Patent number: 10087429
    Abstract: A novel peptide sequence that is a modified derivative of a neuron-specific tyrosine phosphatase is shown and described. Specifically, the novel peptide sequence is a modified derivative of striatal-enriched tyrosine phosphatase (STEP). The peptide sequence has been modified so as to be able to ameliorate and treat brain injury resulting from excessive glutamate release and/or oxidative stress. Examples of the types of brain injury which the presently disclosed peptide sequence is useful for treating includes acute brain injury resulting from stroke or traumatic brain injury and chronic disorders such as Huntington's chorea and schizophrenia. Furthermore, the presently described peptide sequence may further be useful in the treatment and amelioration of disorders associated with fear memory such as post-traumatic stress disorder.
    Type: Grant
    Filed: May 26, 2015
    Date of Patent: October 2, 2018
    Assignee: STC.UNM
    Inventor: Surojit Paul
  • Patent number: 9999887
    Abstract: An apparatus for a test liquid includes an inlet device defining a chamber configured to receive the liquid, a preparation device defining a preparation chamber and including a preparation reagent to be reacted with the liquid, an analysis device defining an exposure chamber associated with the preparation chamber and including an analysis unit to be exposed to the prepared test liquid for indicating information on the test liquid, a housing defining a longitudinal axis, and a guiding device configured to guide the inlet device, the preparation device or the analysis device so as to limit the motion of the inlet device, the preparation device or the analysis device to a sequence of alternating rotational and axial movements, each axial movement of the inlet device, the preparation device or the analysis device requiring activation through a preceding rotational movement of the inlet device, the preparation device or the analysis device.
    Type: Grant
    Filed: February 10, 2015
    Date of Patent: June 19, 2018
    Assignee: SULZER MIXPAC AG
    Inventor: Josef Ettlin
  • Patent number: 9976170
    Abstract: The present invention relates to a method for evaluating the integrity of the cell wall of the bacteria present in a culture in the presence of an antibiotic acting on the bacterial cell wall which, from a practical point of view, allows quickly determining if a bacterium is sensitive or resistant to an antibiotic acting on the bacterial cell wall. Likewise, the present invention also relates to a lysis solution applicable in the preceding method, specifically affecting bacteria having the cell wall damaged by the action of an antibiotic acting on the bacterial cell wall, which allows distinguishing bacteria sensitive to said antibiotic from those resistant to said antibiotic.
    Type: Grant
    Filed: July 26, 2012
    Date of Patent: May 22, 2018
    Assignee: UNIVERSIDAD AUTONOMA DE MADRID
    Inventors: Jose Luis Fernandez Garcia, Jaime Gosalvez Berenguer, German Bou Arevalo, Maria Tamayo Novas, Rebeca Santiso Brandariz
  • Patent number: 9952207
    Abstract: When an immunochromatography reagent composition containing a nonionic surfactant, an N,N-bis(2-hydroxyethyl)glycine buffer, and casein is used as a specimen treatment solution or developing solution in detecting a detection object in a specimen according to an immunochromatographic method, an immunochromatography reagent composition which suppresses a non-specific reaction; does not cause aggregation of antibody-immobilized colloidal gold; and has a high developing rate, in comparison with conventional technology which detects a detection object by using an immunochromatographic device; and a high-performance and highly-sensitivity immunochromatographic method and a detection kit capable of rapid, simple and easy virus detection, which use the immunochromatography reagent composition are provided.
    Type: Grant
    Filed: August 3, 2011
    Date of Patent: April 24, 2018
    Assignee: TANAKA KIKINZOKU KOGYO K.K.
    Inventors: Hisahiko Iwamoto, Yuhiro Sakakibara, Satoru Nakajima
  • Patent number: 9848603
    Abstract: A system for reducing the incidence or severity of fungal infection of a plant, comprising a chemical fungicide component, which may be provided by foliar or root (soil or liquid nutrient) application, together with an antifungal plant defensin not in nature expressed in the plant being protected or expressed in lower amounts or in different tissues, provides synergistic improvement in protection against infection by a plant pathogenic fungus which is susceptible to the defensin and the fungicide. The fungicide can be a strobilurin or a triazole, and the defensin can be selected from a wide range of known defensins, for example, NaD1 and others, or it can be a chimeric defensin engineered for low toxicity to the plant. The defensin can be provided as a protein formulation, optionally together with the fungicide, or it can be provided by recombinant expression in the plant to be protected from fungal infection.
    Type: Grant
    Filed: May 12, 2015
    Date of Patent: December 26, 2017
    Assignee: Hexima Limited
    Inventors: Marilyn Anderson, Robyn Heath, James McKenna, Nicole Van Der Weerden
  • Patent number: 9827222
    Abstract: In certain embodiments, the disclosure relates to methods of treating or preventing nephrogenic diabetes insipidus comprising administering an effective amount of a AMPK activator to a subject in need thereof, wherein In certain embodiments, the AMPK activator is metformin or salt thereof. In certain embodiments, the subject has been diagnosed with nephrogenic diabetes insipidus.
    Type: Grant
    Filed: June 27, 2014
    Date of Patent: November 28, 2017
    Assignee: Emory University
    Inventors: Jeff M. Sands, Mitsi A. Blount, Janet D. Klein
  • Patent number: 9809842
    Abstract: Systems, devices, and methods for accurately imaging chemiluminescence and other luminescence are disclosed. A compact, flat-bed scanner having a light-tight enclosure, one or more detector bars of linear charge-coupled device (CCD) or complementary metal oxide semiconductor (CMOS) imaging chips, and high working numerical aperture (NA) optics scans closely over a sample in one direction and then the opposite direction. Averages or other combinations of intensity readings for each pixel location (x, y) between the two or more passes are averaged together in order to compensate for luminescence that varies over time. On-chip pixel binning and multiple clock frequencies can be used to maximize the signal to noise ratio in a CCD-based scanner.
    Type: Grant
    Filed: March 24, 2014
    Date of Patent: November 7, 2017
    Assignee: LI-COR, Inc.
    Inventors: Ahmed Bouzid, Chris Lesiak, Daniel W. Konz, David L. Franzen, William Biggs, Jon P. Anderson
  • Patent number: 9645136
    Abstract: The invention provides methods of treating a meiotic kinase-associated disease, preferably the meiotic kinase HSET, by administering an inhibitor of the meiotic kinase. Preferably, the disease is associated with the presence of supernumerary centrosomes, such as cancer. Methods of inhibiting the growth of a tumor cell by contacting the cell with an inhibitor of a meiotic kinase, preferably HSET, are also provided. Screening methods for identifying inhibitors of the meiotic kinase HSET are also provided. Methods of selecting subjects for treatment with an inhibitor of a meiotic kinase, such as HSET, are also provided.
    Type: Grant
    Filed: January 7, 2015
    Date of Patent: May 9, 2017
    Assignee: DANA-FARBER CANCER INSTITUTE, INC.
    Inventor: David Pellman
  • Patent number: 9598715
    Abstract: Provided is a method for measuring a color change of an oxidation-reduction indicator, which method is applicable to measurement of the cariogenic bacterial count and the like. A color change of an oxidation-reduction indicator is measured by a method for measuring a color change of an oxidation-reduction indicator, the method comprising reacting a test reagent with a test sample and measuring a color change, wherein the test reagent contains an oxidation-reduction indicator, an oxidation-reduction promoter, and a halogen salt.
    Type: Grant
    Filed: August 17, 2012
    Date of Patent: March 21, 2017
    Assignee: ARKRAY, Inc.
    Inventors: Eiji Nishinaga, Naho Suzuki, Takashi Hama, Isao Fukuta
  • Patent number: 9458210
    Abstract: An immunological measurement is performed using anti-CTP antibody characterized by recognizing an antigenic determinant included in the polypeptide represented by amino acid numbers 118-132 of SEQ ID NO: 1, and reacting with native Cochlin-tomoprotein (CTP).
    Type: Grant
    Filed: April 2, 2012
    Date of Patent: October 4, 2016
    Assignee: SAITAMA MEDICAL UNIVERSITY
    Inventors: Tetsuo Ikezono, Satomi Shikaze
  • Patent number: 9308529
    Abstract: The present invention relates to method of using a microfluidic chip for rapid nucleic acid hybridization, comprising: activating a porous substrate with positive charges; injecting a mixed solution of a test nucleic acid and a nucleic acid probe into the microfluidic chip for maintaining the test nucleic acid hybridized to the nucleic acid probe being absorbed to the periphery of the substrate; continuously washing the microfluidic chip with an anionic surfactant; and detecting the hybridization signals on the substrate after washing for a predetermined time; wherein the activation of the substrate with positive charges allows the test nucleic acid hybridized to the nucleic acid probe to form a micelle during washing and the diffusion of such from the periphery toward the center of the substrate to accelerate. Thus, it is possible to accomplish detection in a very short time for application of specific DNA complementary hybridization.
    Type: Grant
    Filed: April 23, 2014
    Date of Patent: April 12, 2016
    Inventors: Jau-Der Chen, Pei-Tai Chen
  • Patent number: 9290792
    Abstract: The present invention relates to a method for measuring the activity of calcineurin in a biological sample wherein a kinase inhibitor is present in the assay reaction mix.
    Type: Grant
    Filed: February 14, 2013
    Date of Patent: March 22, 2016
    Assignees: UNIVERSITE PARIS DESCARTES, ASSISTANCE PUBLIQUE HOPITAUX DE PARIS
    Inventors: Sylvia Sanquer, Robert Barouki, Laurence Herry
  • Patent number: 9284597
    Abstract: Methods and kits for enzymes involved in post-translational modifications are provided. The methods employ elemental analysis, including ICP-MS. The methods allow for the convenient and accurate analysis of post-translation modifications of substrates by enzymes involved in post-translational modifications, including kinase and phosphatase enyzmes.
    Type: Grant
    Filed: July 10, 2014
    Date of Patent: March 15, 2016
    Assignee: Fluidigm Canada Inc.
    Inventor: Olga Ornatsky
  • Patent number: 9284598
    Abstract: Methods and kits for enzymes involved in post-translational modifications are provided. The methods employ elemental analysis, including ICP-MS. The methods allow for the convenient and accurate analysis of post-translation modifications of substrates by enzymes involved in post-translational modifications, including kinase and phosphatase enyzmes.
    Type: Grant
    Filed: July 10, 2014
    Date of Patent: March 15, 2016
    Assignee: Fluidigm Canada Inc.
    Inventor: Olga Ornatsky
  • Patent number: 9091659
    Abstract: A qualitative and quantitative hydrolase detection method includes providing a caged coelenterazine molecule having a detection molecule bonded between a first bonding site or a second bonding site of a coelenterazine molecule and a steric caging group; activating an uncaged coelenterazine molecule by cleaving the detection molecule in the caged coelenterazine molecule with a hydrolase enzyme; determining a light emission from a coelenterazine-activated bioluminescent enzyme reacting with the uncaged coelenterazine molecule activated in the presence of the hydrolase enzyme; and correlating the light emission for quantitatively and qualitatively determining the presence of the hydrolase enzyme.
    Type: Grant
    Filed: September 9, 2013
    Date of Patent: July 28, 2015
    Assignee: Zymera, Inc.
    Inventors: Sukanta Bhattacharyya, Daniel Sobek
  • Patent number: 9075005
    Abstract: Provided are a cell analyzer, and a cell analysis method. A cell analyzer 1 includes a measurement device 2 for detecting information of each cell from a measurement specimen containing cells harvested from an epithelial tissue, and a data processing device 3 for determining appropriateness of the cell harvesting of parabasal cells and acquiring information related to canceration of the cell based on the information detected by the measurement device 2. The data processing device 3 displays on a display section a dialogue showing “cell harvesting inappropriate” when determined that the harvesting of parabasal cells is inappropriate.
    Type: Grant
    Filed: August 9, 2013
    Date of Patent: July 7, 2015
    Assignee: SYSMEX CORPORATION
    Inventors: Ryuichiro Ebi, Yousuke Tanaka, Junya Inoue, Shigeki Abe
  • Patent number: 9051600
    Abstract: Provided is a method for simply and accurately measuring sphingomyelin in a sample, and a kit therefore. The method is a method for measuring sphingomyelin in a sample, comprising reacting the sample with a phospholipase D which does not react with sphingomyelin and lysophosphatidylcholine but reacts with phosphatidylcholine, a lysophospholipase or a monoglyceride lipase, and a choline oxidase, eliminating the formed hydrogen peroxide, reacting the resultant with a phospholipase D which does not react with glycerol-3-phosphorylcholine and free fatty acid but reacts with sphingomyelin, and a choline oxidase, and measuring the formed hydrogen peroxide.
    Type: Grant
    Filed: July 25, 2012
    Date of Patent: June 9, 2015
    Assignee: KYOWA MEDEX CO., LTD.
    Inventors: Takehide Kimura, Kazuhito Miyauchi, Hideyuki Kuwata
  • Publication number: 20150140124
    Abstract: The present invention relates to the BAD pathway's influence on development, progression, chemo-sensitivity, and overall survival for multiple human cancers and its potential as a therapeutic target to increase chemo-sensitivity. BAD pathway expression was associated with the development and/or progression of breast, colon, and endometrial cancers, relapse-free survival from breast cancer, and overall survival from ovarian, colon, and brain cancers. Expression was also associated with in vitro sensitivity to a range of cytotoxic agents. pBAD levels were higher in cancer versus immortalized normal cells and chemo-resistant versus—sensitive cancer cells and associated with increased cell proliferation.
    Type: Application
    Filed: November 10, 2014
    Publication date: May 21, 2015
    Inventors: Johnathan M. Lancaster, Douglas C. Marchion, Yin Xiong
  • Publication number: 20150132784
    Abstract: A method for detecting a xerophilic microorganism is provided. The method comprises providing a sample to be tested, an aqueous diluent comprising about 1.0 to about 10.2 weight percent glycerol, and a thin film culture device comprising a cold water-reconstitutable medium to facilitate the growth of a microorganism. The method further comprises mixing the sample with the aqueous diluent to form an inoculum, contacting a predefined amount of the inoculum with the reconstitutable medium to form an inoculated thin film culture device, incubating the inoculated thin film culture device for a period of time, and detecting a presence or an absence of colony of a microorganism. Kits for detecting a xerophilic microorganism according to the method are also provided.
    Type: Application
    Filed: May 29, 2013
    Publication date: May 14, 2015
    Inventors: Sailaja Chandrapati, Tera M. Nordby
  • Patent number: 9017962
    Abstract: The invention provides a variety of molecular tools for use in live-cell tracking of activities of biomolecules and in high-throughput drug screening.
    Type: Grant
    Filed: August 20, 2008
    Date of Patent: April 28, 2015
    Assignee: The Johns Hopkins University
    Inventors: Jin Zhang, Robert Howard Newman, Xinxin Gao, Michael David Allen, Matthew David Fosbrink
  • Publication number: 20150111228
    Abstract: The present disclosure provides an optical biosensor including a complex including a porous support and an enzyme supported inside the pores of the porous support and a method for preparing the same. Since a dye does not flow but is concentrated in one place, the optical biosensor of the present disclosure has remarkably improved sensitivity. In addition, it is possible to carry out quantitative determination and qualitative analysis since color intensity increases with time.
    Type: Application
    Filed: May 23, 2013
    Publication date: April 23, 2015
    Applicants: KOREA UNIVERSITY RESEARCH AND BUSINESS FOUNDATION, GWANGJU INSTITUTE OF SCIENCE AND TECHNOLOGY
    Inventors: Jungbae Kim, Min-Gon Kim, Hyou-Arm Joung, Yongho Wee
  • Patent number: 9012145
    Abstract: Tyrosine phosphorylation, regulated by protein tyrosine phosphatases (PTPs) and kinases (PTKs), is important in signaling pathways underlying tumorigenesis. A mutational analysis of the tyrosine phosphatase gene superfamily in human cancers identified 83 somatic mutations in six PTPs (PTPRF, PTPRG, PTPRT, PTPN3, PTPN13, PTPN14), affecting 26% of colorectal cancers and a smaller fraction of lung, breast and gastric cancers. Fifteen mutations were nonsense, frameshift or splice site alterations predicted to result in truncated proteins lacking phosphatase activity. Five missense mutations in the most commonly altered PTP (PTPRT) were biochemically examined and found to reduce phosphatase activity. Expression of wild-type but not a mutant PTPRT in human cancer cells inhibited cell growth. These observations suggest that the tyrosine phosphatase genes are tumor suppressor genes, regulating cellular pathways that may be amenable to therapeutic intervention.
    Type: Grant
    Filed: October 18, 2011
    Date of Patent: April 21, 2015
    Assignee: The Johns Hopkins University
    Inventors: Zhenghe Wang, Victor Velculescu, Kenneth W. Kinzler, Bert Vogelstein
  • Publication number: 20150093771
    Abstract: An analytical device for analysis of chemical or biological samples, a method of using such a device, based on rotation of the device, integrated sample dosing and optical detection, and a system comprising such a device are disclosed. The analytical device comprises a device body having a liquid processing unit. The liquid processing unit comprises a mixing chamber for mixing a sample with a reagent, a sample dosing chamber for delivering a defined volume of the sample to the mixing chamber, and a reagent channel for delivering the reagent to be mixed with the sample, wherein the mixing chamber also serves as a detection chamber.
    Type: Application
    Filed: December 2, 2014
    Publication date: April 2, 2015
    Inventors: Patrick Griss, Rainer Jaeggi, Goran Savatic, Vuk Siljegovic
  • Publication number: 20150087552
    Abstract: In one aspect, the invention relates to a method for identifying a compound which modulates the activity of a voltage-gated protein. In certain embodiments, the voltage gate protein is a voltage-gated ion channel. In certain embodiments, the voltage-gated protein is a voltage sensitive phosphatase. In certain embodiments, the voltage-gated protein used in conjunction with the methods of the invention is modified to altered permeability or voltage sensitivity.
    Type: Application
    Filed: August 29, 2014
    Publication date: March 26, 2015
    Inventors: Morten Ostergaard JENSEN, David BORHANI, VISHWANATH Jogini
  • Publication number: 20150079617
    Abstract: The invention relates to a method for determining biochemical parameters of a body fluid, wherein a sample of said body fluid in the form of a droplet is transported through a channel of a microfluidic system using a carrier liquid, mixed with a reagent thus initiating a chemical reaction between the sample and the reagent, and the result of the chemical reaction is measured, preferably with a spectrophotometer, whereby the said biochemical parameters of the body fluid are determined, characterised in that the material used for fabrication of the microfluidic system and the said carrier liquid is pair of Teflon and Fluorinert HFE-7100.
    Type: Application
    Filed: September 12, 2012
    Publication date: March 19, 2015
    Inventors: Piotr Garstecki, Marcin Izydorzak, Magdalena Sulima, Adam Warchulski
  • Patent number: 8980544
    Abstract: A boronic or borinic acid compound is used to inhibit the activity of a sulfenic acid-containing protein. Thus, a biologically-active sulfenic acid-containing protein is contacted with an activity-inhibiting effective amount of a boronic or borinic acid compound of Formula I or a salt, hydrate or solvate thereof, whose components are disclosed within, and that contact is maintained for a time sufficient to inhibit the biological activity of the protein.
    Type: Grant
    Filed: August 27, 2013
    Date of Patent: March 17, 2015
    Assignee: The Penn State Research Foundation
    Inventors: Stephen Benkovic, Chunyu Liu, John W. Tomsho
  • Publication number: 20150071905
    Abstract: High affinity SIRP-? reagent are provided, which (i) comprise at least one amino acid change relative to the wild-type protein; and (ii) have an increased affinity for CD47 relative to the wild-type protein. Compositions and methods are provided for modulating phagocytosis in a mammal by administering a therapeutic dose of a pharmaceutical composition comprising a high affinity SIRP? reagent, which blocks the physiological binding interaction between SIRP? and its ligand CD47.
    Type: Application
    Filed: January 17, 2013
    Publication date: March 12, 2015
    Inventors: Aaron Michael Ring, Kenan Christopher Garcia, Kipp Andrew Weiskopf, Aron M. Levin, Irving L. Weissman
  • Publication number: 20150064192
    Abstract: Purified genes encoding a T cell surface antigen from a mammal, reagents related thereto including purified proteins, specific antibodies, and nucleic acids encoding this antigen are provided. Methods of using said reagents and diagnostic kits are also provided.
    Type: Application
    Filed: November 14, 2014
    Publication date: March 5, 2015
    Inventors: DANIEL M. GORMAN, TROY D. RANDALL, ALBERT ZLOTNIK
  • Publication number: 20150050681
    Abstract: The present invention relates to a method for measuring the activity of calcineurin in a biological sample wherein a kinase inhibitor is present in the assay reaction mix.
    Type: Application
    Filed: February 14, 2013
    Publication date: February 19, 2015
    Applicant: UNIVERSITE PARIS DESCARTES
    Inventors: Sylvia Sanquer, Robert Barouki, Laurence Herry
  • Publication number: 20150051097
    Abstract: Methods of screening for candidate compounds capable of inhibiting activity of fyn in phosphorylating tau protein at Y394 or binding to fyn to inhibit interaction with tau protein at Y394, including determining whether, and optionally the extent, the candidate compounds have these capabilities under conditions where fyn has these capabilities in the absence of the candidate compound. Methods of screening for substances capable of promoting dephosphorylation of tau protein by a phosphatase at a site of tau protein including contacting a candidate substance, the tau protein and a phosphatase capable of dephosphorylating the tau protein under conditions where the phosphatase is capable of dephosphorylating the site in absence of the candidate substance, where the kinase is fyn; determining whether, and optionally the extent, the candidate substance promotes dephosphorylation of the tau protein at the site; and selecting the candidate substance which promotes dephosphorylation of the tau protein the sites.
    Type: Application
    Filed: July 24, 2014
    Publication date: February 19, 2015
    Applicants: Proteome Sciences PLC, King's College London
    Inventors: Brian Anderton, Diane Hanger, Malcolm Ward, Helen Byers
  • Patent number: 8956824
    Abstract: This invention provides a method for identifying allosteric modulators of a SHIP polypeptide, wherein said SHIP polypeptide is contacted with a test compound, and wherein said SHIP polypeptide comprises an allosteric site selected from the group consisting of a SHIP C2 domain and a SHIP PH domain.
    Type: Grant
    Filed: June 4, 2009
    Date of Patent: February 17, 2015
    Assignee: British Columbia Cancer Agency Branch
    Inventors: Alice Mui, Christopher Ong, Gerald Krystal, Raymond Andersen
  • Patent number: 8951748
    Abstract: Methods and kits are disclosed for detecting microorganisms that produce glucose oxidase. The method includes providing a culture medium and a hydrogen peroxide indicating reagent comprising a chromogenic substrate that can provide a detectable chromogenic reaction indicating the presence of a microorganism that produces glucose oxidase, and additional methods are disclosed for differentiating microorganisms by the detection of an additional chromogenic reaction.
    Type: Grant
    Filed: December 21, 2010
    Date of Patent: February 10, 2015
    Assignee: 3M Innovative Properties Company
    Inventors: Stephen B. Roscoe, Michael J. Svarovsky, Stephanie J. Moeller
  • Publication number: 20150038551
    Abstract: The present invention relates to a pharmaceutical composition for preventing or treating diabetes or a complication of diabetes, which includes a TENC1 (tensin like C1 domain-containing phosphatase) expression or activity suppressor, and, more specifically, relates to a pharmaceutical composition for preventing or treating diabetes or a complication of diabetes, which either suppresses the degradation of IRS-1 (insulin receptor substrate-1) or suppresses the phosphorylation of IRS-1 due to the PTPase activity of TENC1.
    Type: Application
    Filed: January 14, 2013
    Publication date: February 5, 2015
    Applicant: POSTECH ACADEMY-INDUSTRY FOUNDATION
    Inventors: Sung Ho Ryu, A. Ra Koh, Mi Nam Lee, Hee Yoon Jeong, Yong Ryoul Yang, Pann Ghill Suh
  • Publication number: 20150011422
    Abstract: Methods and kits for enzymes involved in post-translational modifications are provided. The methods employ elemental analysis, including ICP-MS.
    Type: Application
    Filed: July 10, 2014
    Publication date: January 8, 2015
    Applicant: Fluidigm Canada Inc.
    Inventor: Olga Ornatsky
  • Publication number: 20150005193
    Abstract: Disclosed herein are “equipment-free” flow-through assay devices based on patterned porous media, methods of making same, and methods of using same. The porous, hydrophilic media are patterned with hydrophobic barriers for performing assays on liquids.
    Type: Application
    Filed: June 20, 2014
    Publication date: January 1, 2015
    Inventors: Scott Thomas Phillips, Gregory Gerald Lewis, Jessica Sloane Robbins
  • Publication number: 20140370501
    Abstract: The present invention provides novel methods for determining the presence or amount of a hydrolytic enzyme in a sample, based on novel substrates for the enzymes, and also provides compositions and methods that provide highly sensitive assay methods for such hydrolytic enzymes.
    Type: Application
    Filed: June 19, 2014
    Publication date: December 18, 2014
    Inventors: Chong-Sheng YUAN, Xiaoru CHEN
  • Publication number: 20140357526
    Abstract: A method for analyzing the polypeptide content of animal tissue is described. The method includes the steps of (a) providing an animal tissue specimen; (b) depositing one or more portions of a hydrogel mixture including a protease on spatially discrete portions of the animal tissue specimen; (c) allowing sufficient time to pass for animal tissue under the hydrogel mixture to be form a digested mixture of animal tissue and hydrogel mixture; (d) removing the digested mixture from the animal tissue and extracting the polypeptides from the digested mixture to provide an extract; and (e) analyzing the polypeptide content of the extract by mass spectrometry.
    Type: Application
    Filed: June 2, 2014
    Publication date: December 4, 2014
    Applicant: Vanderbilt University
    Inventors: Richard Caprioli, Glenn A. Harris, Joshua J. Nicklay
  • Publication number: 20140336202
    Abstract: Methods of treating subjects with cancer and screening for MDM2 inhibitors that may be effective cancer therapeutics are provided herein. The cancers that may be treated using MDM2 inhibitors using the methods described herein include those that are or may become resistant to treatment with tyrosine kinase inhibitors. Methods of treating subjects with cancers that have, or develop in response to treatment with tyrosine kinase inhibitors, elevated levels of MDM2, Mcl-1 or PP5 or decreased levels of Huwe1 or CAS using MDM2 inhibitors are provided herein. The MDM2 inhibitors may be effective at treating these cancers alone or in combination with a tyrosine kinase inhibitor regardless of p53 status (mutant or wild-type) of the cancer.
    Type: Application
    Filed: December 7, 2012
    Publication date: November 13, 2014
    Applicant: DUKE UNIVERSITY
    Inventors: Sally Kornbluth, Manabu Kurokawa, Neil Spector, Mark Dewhirst
  • Publication number: 20140323339
    Abstract: Methods and kits for enzymes involved in post-translational modifications are provided. The methods employ elemental analysis, including ICP-MS.
    Type: Application
    Filed: July 10, 2014
    Publication date: October 30, 2014
    Applicant: FLUIDIGM CANADA INC.
    Inventor: Olga Ornatsky
  • Patent number: 8865420
    Abstract: The present invention provides an approach for the simultaneous determination of the activation states of a plurality of proteins in single cells. This approach permits the rapid detection of heterogeneity in a complex cell population based on activation states, and the identification of cellular subsets that exhibit correlated changes in activation within the cell population. Moreover, this approach allows the correlation of cellular activities or properties. In addition, the use of potentiators of cellular activation allows for characterization of such pathways and cell populations.
    Type: Grant
    Filed: May 22, 2008
    Date of Patent: October 21, 2014
    Assignee: The Board of Trustees of the Leland Stanford Junior University
    Inventors: Omar D. Perez, Garry P. Nolan, Jonathan M. Irish
  • Patent number: 8859220
    Abstract: A method and kit are provided for enhancing the tolerance of an assay reagent to compounds in an assay sample, the assay reagent including a luciferase enzyme. The method includes contacting the luciferase with a tolerance enhancement agent in an amount sufficient to substantially protect luciferase enzyme activity from interference of the compound and minimize interference by at least about 10% relative to an assay not having tolerance enhancement agent.
    Type: Grant
    Filed: January 25, 2013
    Date of Patent: October 14, 2014
    Assignee: Promega Corporation
    Inventors: Erika Hawkins, James J. Cali, Samuel Kin Sang Ho, Martha A. O'Brien, Richard Somberg, Robert F. Bulleit, Keith V. Wood
  • Publication number: 20140287943
    Abstract: The present disclosure is directed to detecting colon disorders by measuring the expression of SHIP1 in a sample of PBMCs. One method includes the following steps, obtaining a sample including peripheral blood mononuclear cells (PBMCs) from a subject and determining whether SHIP1 is underexpressed in the PBMCs or lacks normal enzymatic activity. The present disclosure is also directed to a method of determining the expression of SHIP1 protein expression and SHIP1 enzyme activity in PBMCs. This method includes the following steps, obtaining a sample comprising PBMCs from a subject and determining the amount of SHIP1 in the PBMCs.
    Type: Application
    Filed: April 27, 2012
    Publication date: September 25, 2014
    Applicants: ERASMUS UNIVERSITY MEDICAL CENTER ROTTERDAM, THE RESEARCH FOUNDATION FOR THE STATE UNIVERSITY OF NEW YORK
    Inventors: William G. Kerr, Gwenny M. Fuhler, Sandra Fernandes
  • Publication number: 20140273009
    Abstract: The disclosure relates to compositions for use in assays, the compositions comprising at least one latent fluorophore including at least one enzyme-reactive quenching group and a conjugative group; and a support connectable to the latent fluorophore by the conjugative group. The disclosure further relates to methods of measuring the presence and/or concentration of an analyte, as well as methods of measuring the relative activity of at least two enzymes.
    Type: Application
    Filed: March 14, 2013
    Publication date: September 18, 2014
    Applicant: Palo Alto Research Center Incorporated
    Inventors: Michael I. RECHT, Peter KIESEL, Joerg MARTINI, Francisco E. TORRES
  • Publication number: 20140255952
    Abstract: The invention relates to carbon nanotube-containing composites as biosensors to detect the presence of target clinical markers, methods of their preparation and uses in the medical field. The invention is particularly suitable for the detection in patient biological specimens of bone markers and tissue markers. The biosensors of the invention include carbon nanotubes deposited on a substrate, gold nanoparticles deposited on the carbon nanotubes and, binder material and biomolecule deposited on the gold-coated carbon nanotubes. The biomolecule is selected to interact with the target clinical markers. The biosensor can be used as an in-situ or an ex-situ device to detect and measure the presence of the target clinical markers.
    Type: Application
    Filed: March 5, 2014
    Publication date: September 11, 2014
    Applicant: UNIVERSITY OF PITTSBURGH - OF THE COMMONWEALTH SYSTEM OF HIGHER EDUCATION
    Inventors: PRASHANT NAGESH KUMTA, MADHUMATI RAMANATHAN, MITALI SHIRISH PATIL
  • Publication number: 20140249150
    Abstract: The invention described herein features methods, compositions, and kits that utilize activators of pyruvate kinase M2 (PKM2) for the treatment or amelioration of disorders related to PKM2 function and characterized by abnormally low levels of serine.
    Type: Application
    Filed: October 12, 2012
    Publication date: September 4, 2014
    Applicant: AGIOS PHARMACEUTICALS, INC
    Inventor: Charles Kung
  • Publication number: 20140242092
    Abstract: We provide VHZ for use in a method of treatment, prophylaxis or alleviation of a cancer, such as breast cancer, in an individual. We provide an anti-VHZ agent for the treatment, prophylaxis or alleviation of cancer. We further provide a kit for detecting breast cancer in an individual or susceptibility of the individual to breast cancer comprising means for detection of VHZ expression in the individual or a sample taken from him or her as well as a method of detecting a cancer cell, the method comprising detecting modulation of expression, amount or activity of VHZ in the cell.
    Type: Application
    Filed: May 13, 2014
    Publication date: August 28, 2014
    Applicant: Agency For Science, Technology And Research (A*STAR)
    Inventor: Qi Zeng
  • Publication number: 20140242595
    Abstract: The present invention provides methods comprising both genetic and chemical means for the production of hepatocytes from a variety of cell sources, particularly pluripotent stem cells.
    Type: Application
    Filed: February 21, 2014
    Publication date: August 28, 2014
    Applicant: CELLULAR DYNAMICS INTERNATIONAL, INC.
    Inventors: Junying YU, Xin ZHANG
  • Patent number: 8802389
    Abstract: The subject invention relates in part to the surprising and unexpected discovery that insects that are resistant to Bacillus thuringiensis Cry toxins have measurably altered alkaline phosphatase (ALP) activity as compared to insects that are susceptible to Cry toxins. This and other surprising discoveries reported herein have broad implications in areas such as managing and monitoring the development of insect resistance to B.t. toxins. For example, the subject invention provides a simple and fast assay (enzymatic or otherwise) for detecting ALP activity levels and thereby monitoring the development of resistance by insects to crystal protein insect toxins. There was no prior motivation or suggestion to go about resistance monitoring using this simple and easy approach.
    Type: Grant
    Filed: October 17, 2011
    Date of Patent: August 12, 2014
    Assignee: University of Georgia Research Foundation, Inc.
    Inventors: Michael J. Adang, Rebecca McNall, Juan Luis Jurat Fuentes
  • Publication number: 20140220610
    Abstract: A thin film culture device for detecting yeast and mold microorganisms in a sample is provided. The culture device comprises a body comprising a self-supporting substrate having a first major surface and a second major surface; a first adhesive composition disposed on a portion of the first major surface of the substrate; a substantially dry, cold-water-soluble first hydrogel-forming composition adhered to the first adhesive composition; and a plurality of indicator agents. The plurality of indicator agents comprises three indicator agents for detecting distinct glycosidase enzyme activities, an indicator agent for detecting an alkyl esterase enzyme activity, and an indicator agent for detecting a phosphatase enzyme activity, wherein each of the plurality of indicator agents comprises a detectable reporter group. A method of using the culture device is also provided.
    Type: Application
    Filed: February 25, 2013
    Publication date: August 7, 2014
    Applicant: 3M INNOVATIVE PROPERTIES COMPANY
    Inventors: SAILAJA CHANDRAPATI, TERA M. NORDBY
  • Patent number: 8790886
    Abstract: Methods to assay kinase activity are provided herein. The methods employ elemental analysis, including inductively charged plasma mass spectrometry (ICP-MS). The methods allow for the convenient and accurate analysis of post-translation modifications of substrates by kinase enzymes involved in post-translational modifications.
    Type: Grant
    Filed: November 21, 2012
    Date of Patent: July 29, 2014
    Assignee: Fluidigm Canada Inc.
    Inventor: Olga Ornatsky