Acting On Choh Group As Donor (e.g., Glucose Oxidase, Lactate Dehydrogenase (1.1)) Patents (Class 435/190)
  • Patent number: 10961514
    Abstract: The present invention relates to a protein having glucose dehydrogenase activity selected from: (a) an amino acid sequence represented by SEQ ID NO: 3; (b) an amino acid sequence in which 1 to 3 amino acids are deleted from, replaced in or added to the amino acid sequence represented by SEQ ID NO: 3; (c) an amino acid sequence which has at least 80% identity with the amino acid sequence represented by SEQ ID NO: 3 and whose N-terminus is SS; or (d) an amino acid sequence which has at least 80% identity with the amino acid sequence represented by SEQ ID NO: 3, and does not contain a sequence represented by SEQ ID NO: 8 at its N-terminus. The invention also includes a polynucleotide encoding the enzyme, a method for manufacturing the enzyme, a method for measuring glucose using the enzyme, a measuring reagent composition and a biosensor.
    Type: Grant
    Filed: January 11, 2017
    Date of Patent: March 30, 2021
    Assignee: IKEDA FOOD RESEARCH CO., LTD.
    Inventors: Ryo Takenaka, Takafumi Takumi
  • Patent number: 10920200
    Abstract: Provided are a glucose oxidase CnGODA, an encoding gene thereof, a recombinant expression vector comprising the gene, and a recombinant strain; the amino acid sequence of the glucose oxidase CnGODA is as represented in SEQ ID NO:1 or SEQ ID NO:2. Further provided is a method for use in preparing glucose oxidase CnGODA, and application of glucose oxidase CnGODA.
    Type: Grant
    Filed: June 7, 2018
    Date of Patent: February 16, 2021
    Assignee: FEED RESEARCH INSTITUTE, CHINESE ACADEMY OF AGRICULTURAL SCIENCES
    Inventors: Bin Yao, Huiying Luo, Weina Liu, Jianzhong Ge, Tao Tu, Huoqing Huang, Xiaoyun Su, Yingguo Bai, Yuan Wang, Yaru Wang, Kun Meng
  • Patent number: 10884001
    Abstract: Featured are methods of diagnosing and treating liver disease.
    Type: Grant
    Filed: January 9, 2015
    Date of Patent: January 5, 2021
    Assignee: Beth Israel Deaconess Medical Center, Inc.
    Inventors: Detlef Schuppan, Towia Libermann, Simon T. Dillon, Yury Papou
  • Patent number: 10870835
    Abstract: The disclosure relates to engineered ketoreductase polypeptides and processes of using the polypeptides for production of phenylephrine.
    Type: Grant
    Filed: January 31, 2020
    Date of Patent: December 22, 2020
    Assignee: Codexis, Inc.
    Inventors: Oscar Alvizo, Steven J. Collier, Hans-Georg Joerg Hennemann, Seong Ho Oh, Wenjuan Zha
  • Patent number: 10597685
    Abstract: The present invention provides for a method of producing an oxidation product of an aromatic amino acid in a genetically modified host cell. The method comprises culturing the genetically modified host cell under a suitable condition such that the culturing results in the genetically modified host cell producing oxidation product of an aromatic amino acid. The host cell comprises an enzyme capable of catalyzing the oxidation of aromatic amino acid. In some embodiments, the host cell is capable of biosynthesizing BH4 or MH4 from GTP.
    Type: Grant
    Filed: September 30, 2013
    Date of Patent: March 24, 2020
    Assignee: The Regents of the University of California
    Inventors: Taek Soon Lee, Yasuharu Satoh, Jay D. Keasling
  • Patent number: 10457965
    Abstract: The invention relates to a cell expressing a polypeptide having 5-hydroxymethyl-2-furancarboxylic acid dehydrogenase activity, as well as to a cell expressing a polypeptide having furanic compound transport capabilities. The invention also relates to a process for the production of 2,5-furan-dicarboxylic acid (FDCA) wherein the cells of the invention are used for oxidation of a furanic precursors of FDCA.
    Type: Grant
    Filed: February 17, 2016
    Date of Patent: October 29, 2019
    Assignee: PURAC BIOCHEM B.V.
    Inventor: Harald Johan Ruijssenaars
  • Patent number: 10450555
    Abstract: The present invention relates to multi-hapten mutant G6PDH conjugates, methods of their preparation and their use for detection of multiple analytes. The compositions of the invention comprise different types of haptens (molecules) require to be immobilized into the G6PDH to make one multi-hapten-G6PDH conjugate. Both thiol and amine functional groups on G6PDH are utilized for immobilization of different haptens.
    Type: Grant
    Filed: November 2, 2018
    Date of Patent: October 22, 2019
    Assignee: Siemens Healthcare Diagnostics Inc.
    Inventors: Yi Feng Zheng, Yali Yang, Linda Hudson, Nihmat Morjana
  • Patent number: 10426111
    Abstract: Disclosed is an invention of cotton lines 2-340/1-1422, 1-1422/SCM 3-7-3, 1-1422/2-340, 1-136/2-340, AFIS 1-1422. AFIS 2-340, AFIS 1-136, and SCM 3-7-3, and relates to seeds, plants, plant cells, plant tissue and harvested products, lint, and oil, as well as to hybrid cotton plants and seeds and other plants, cultivars, and varieties produced by essentially deriving such plants, cultivars, and varieties from cotton lines 2-340/1-1422, 1-1422/SCM 3-7-3, 1-1422/2-340, 1-136/2-340, AFIS 1-1422, AFIS 2-340, AFIS 1-136, and SCM 3-7-3. The present invention particularly relates to the development of specific cotton lines for the regulation of palmitic acid for the production of seeds having cottonseed oil content with reduced levels of palmitic acid. The cotton lines of the present disclosure have lower levels of palmitic acid content, and allows for the germination of seed at lower soil temperatures than current commercially available cotton cultivars.
    Type: Grant
    Filed: September 8, 2014
    Date of Patent: October 1, 2019
    Assignee: TEXAS TECH UNIVERSITY SYSTEM
    Inventors: Dick L. Auld, Bralie Renae Hendon
  • Patent number: 10385371
    Abstract: The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize chiral compounds.
    Type: Grant
    Filed: February 22, 2018
    Date of Patent: August 20, 2019
    Assignee: Codexis, Inc.
    Inventors: Jack S Liang, Stephan Jenne, Emily Mundorff, Rama Voladri, James J. Lalonde, Gjalt W. Huisman
  • Patent number: 10351892
    Abstract: The present invention provides a mutant-type glucose dehydrogenase having glucose dehydrogenase activity and having decreased reactivity with xylose, wherein said mutant-type glucose dehydrogenase comprises a mutant-type ?-subunit comprising an amino acid sequence of 520 to 550 amino acids comprising an amino acid sequence of 520 to 550 amino acids comprising SEQ ID NO: 21, SEQ ID NO: 22, SEQ ID NO: 23, SEQ ID NO: 24 and SEQ ID NO: 25 in this order from N-terminus to C-terminus, except that one or more amino acid residue(s) selected from the group consisting of the glycine at position 10 in SEQ ID NO: 23, the histidine at position 4 in SEQ ID NO: 24, and the asparagine at position 4 in SEQ ID NO: 25 is/are substituted with another/other amino acid(s).
    Type: Grant
    Filed: October 28, 2016
    Date of Patent: July 16, 2019
    Assignees: ARKRAY, Inc., Ultizyme International Ltd.
    Inventors: Katsuhiro Kojima, Kazushige Mori
  • Patent number: 10240173
    Abstract: The invention described materials and methods for producing oxidized 13R-MO, such as forskolin. In particular, the invention describes P450s involved in oxidation of 13R-MO including CYP76AH8, CYP76AH11, CYP76AH15, CYP76AH17, CYP71D381 and CYP76AH9. Host organisms expressing one or more of these P450s are useful in the production of oxidized 13R-MO.
    Type: Grant
    Filed: July 10, 2018
    Date of Patent: March 26, 2019
    Assignees: University of Copenhagen, Danmarks Tekniske Universitet, Evolva SA
    Inventors: Björn Hamberger, Birger Lindberg Møller, Eirini Pateraki, Johan Andersen-Ranberg, Niels Bjerg Jensen
  • Patent number: 10053717
    Abstract: The invention described materials and methods for producing oxidized 13R-MO, such as forskolin. In particular, the invention describes P450s involved in oxidation of 13R-MO including CYP76AH8, CYP76AH11, CYP76AH15, CYP76AH17, CYP71D381 and CYP76AH9. Host organisms expressing one or more of these P450s are useful in the production of oxidized 13R-MO.
    Type: Grant
    Filed: January 30, 2015
    Date of Patent: August 21, 2018
    Assignees: University of Copenhagen, Danmarks Tekniske Universitet, Evolva SA
    Inventors: Björn Hamberger, Birger Lindberg Møller, Eirini Pateraki, Johan Andersen-Ranberg, Niels Bjerg Jensen
  • Patent number: 10011846
    Abstract: The present invention provides a bio-fuel, wood or other product, such as a paper, textile or yarn product. The product can contain material from a transgenic plant over-expressing a nucleic acid molecule encoding an enzyme that causes the plant cell wall to be more water soluble than the wild type.
    Type: Grant
    Filed: March 26, 2008
    Date of Patent: July 3, 2018
    Assignees: Futuragene Israel Ltd., Yissum Research Development Company of the Hebrew University of Jerusalem Ltd.
    Inventors: Oded Shoseyov, Ziv Shani, Miron Abramson
  • Patent number: 9994879
    Abstract: The invention relates to a micro-organism comprising a hydrogenase enzyme system which is capable of converting carbon dioxide into formic acid and a second enzyme system which is capable of converting formic acid into aliphatic carboxylic acids having a chain length of five or more carbon atoms. Also described are various methods for producing oil, as well as other aspects of the invention.
    Type: Grant
    Filed: October 7, 2016
    Date of Patent: June 12, 2018
    Assignee: VERDANT BIOPRODUCTS LIMITED
    Inventor: Irene Finnegan
  • Patent number: 9994827
    Abstract: Object An object of the present invention is to provide a highly stable FVHO preparation and a low-hygroscopicity dried FVHO preparation. Means for achieving the object A method for producing a FVHO preparation comprising a step of allowing at least one member selected from phosphoric acid, casein peptone, D-glucosamine hydrochloride, melibiose, sorbose, lactose, fructose, melezitose, glucono-1,5-lactone, and ribitol; and a method for producing a dried FVHO preparation, comprising a step of allowing Bicine to coexist.
    Type: Grant
    Filed: January 10, 2013
    Date of Patent: June 12, 2018
    Assignee: TOYOBO CO., LTD.
    Inventors: Rie Hirao, Takahide Kishimoto, Shusaku Yanagidani
  • Patent number: 9944970
    Abstract: The present invention provides a protein comprising an amino acid sequence in which arginine at position 61 of a protein comprising the amino acid sequence represented by SEQ ID NO: 1 is substituted to an amino acid selected from the group consisting of glycine, alanine, valine, leucine, serine, threonine, proline, cysteine, methionine, asparagine, glutamine, and aspartic acid; and a method for measuring a glycated hemoglobin in a sample, wherein the method comprises reacting a glycated hemoglobin in a sample with a protease to produce a glycated hexapeptide, then reacting the produced glycated hexapeptide with the aforementioned protein, and measuring a substance produced or consumed by the reaction.
    Type: Grant
    Filed: July 7, 2014
    Date of Patent: April 17, 2018
    Assignees: KYOWA MEDEX CO., LTD., KYOTO UNIVERSITY
    Inventors: Noriyuki Ogawa, Fumi Umehara, Takehide Kimura, Kousaku Murata, Wataru Hashimoto
  • Patent number: 9873864
    Abstract: The present invention relates to cellobiose dehydrogenases (CDH) having glucose oxidation activity at a pH of 7.4 or above, modifications to modify the pH dependency of the enzymes activity, uses for these CDHs, in particular electrode sensors and electrochemical cells.
    Type: Grant
    Filed: May 12, 2017
    Date of Patent: January 23, 2018
    Assignee: DIRECTSENS GMBH
    Inventors: Roland Ludwig, Dietmar Haltrich, Wolfgang Harreither, Lo Gorton
  • Patent number: 9796964
    Abstract: The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme including the capability of reducing 5-((4S)-2-oxo-4-phenyl (1,3-oxazolidin-3-yl))-1-(4-fluorophenyl) pentane-1,5-dione to (4S)-3-[(5S)-5-(4-fluorophenyl)-5-hydroxypentanoyl]-4-phenyl-1,3-oxazolidin-2-one. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize the intermediate (4S)-3-[(5S)-5-(4-fluorophenyl)-5-hydroxypentanoyl]-4-phenyl-1,3-oxazolidin-2-one in a process for making Ezetimibe.
    Type: Grant
    Filed: July 15, 2016
    Date of Patent: October 24, 2017
    Assignee: Codexis, Inc.
    Inventors: Emily Mundorff, Erik Jan De Vries
  • Patent number: 9725701
    Abstract: The present invention relates to transgenic plants that have increased nitrogen use efficiency, stress tolerance, and/or alleviating a limitation such that yield is increased, or a combination of these and that have been transformed using a novel vector construct including a synthetic isocitrate dehydrogenase (icdh) gene that modulates nitrogen use in plants. The invention also relates to stacking the icdh gene with other exogenous or heterologous genes that modulate nitrogen use in the plant, including a N-acetylglutamate kinase gene. The invention also relates to methods of expressing in plants the nucleic acid molecules corresponding to the nucleic acid sequences that modulate nitrogen use in plants or are modulated by nitrogen conditions.
    Type: Grant
    Filed: March 15, 2013
    Date of Patent: August 8, 2017
    Assignee: Iowa Corn Promotion Board
    Inventors: James McLaren, Brian Vande Berg
  • Patent number: 9663767
    Abstract: The present invention relates to cellobiose dehydrogenases (CDH) having glucose oxidation activity at a pH of 7.4 or above, modifications to modify the pH dependency of the enzymes activity, uses for these CDHs, in particular electrode sensors and electrochemical cells.
    Type: Grant
    Filed: June 4, 2015
    Date of Patent: May 30, 2017
    Assignee: DIRECTSENS GMBH
    Inventors: Roland Ludwig, Dietmar Haltrich, Wolfgang Harreither, Lo Gorton
  • Patent number: 9624517
    Abstract: Materials and methods are described to produce xylitol from a mixture of hemicellulosic sugars by several routes. Examples include either as a direct co-product of a biorefinery or ethanol facility, or as a stand-alone product produced from an agricultural or forestry biomass feedstock including using, e.g. ethanol waste streams.
    Type: Grant
    Filed: July 20, 2011
    Date of Patent: April 18, 2017
    Assignees: The Board of Trustees of the University of Illinois, ZuChem, Inc.
    Inventors: Huimin Zhao, Nikhil Unni Nair, Michael Racine, Ryan Woodyer
  • Patent number: 9611487
    Abstract: The present disclosure relates, in some aspects, to cell-free methods and systems for large-scale conversion of methane to isobutanol, comprising combining, in a bioreactor at elevated pressure, methane, oxygen, and cell lysates containing methane monooxygenase, methanol dehydrogenase, and enzymes that catalyze the conversion of formaldehyde to isobutanol, to form a cell-free reaction mixture, and incubating under suitable conditions the cell-free reaction to convert methane to isobutanol.
    Type: Grant
    Filed: December 20, 2013
    Date of Patent: April 4, 2017
    Assignee: GreenLight Biosciences, Inc.
    Inventors: William Jeremy Blake, James R. Swartz
  • Patent number: 9593326
    Abstract: Region shuffling methods for efficiently introducing diversity and exploring sequence space are described. Libraries produced directly from these methods contain high fractions of protein variants harboring multiple beneficial mutations. Typically, the methods produce these variants efficiently without the need for sequencing beneficial mutants identified at intermediate stages of the process.
    Type: Grant
    Filed: June 25, 2012
    Date of Patent: March 14, 2017
    Assignee: Codexis, Inc.
    Inventors: Louis Clark, Trish Choudhary
  • Patent number: 9422531
    Abstract: The present disclosure relates to engineered ketoreductase polypeptides for the preparation of hydroxyl substituted carbamate compounds, and polynucleotides, vectors, host cells, and methods of making and using the ketoreductase polypeptides.
    Type: Grant
    Filed: August 13, 2015
    Date of Patent: August 23, 2016
    Assignee: Codexis, Inc.
    Inventors: Fabien Louis Cabirol, Haibin Chen, Anupam Gohel, Steven J. Collier, Derek J. Smith, Birgit Kosjek, Jacob Janey
  • Patent number: 9404144
    Abstract: An object of the present invention is to provide a novel glucose dehydrogenase, a method for producing the glucose dehydrogenase, and applications of the glucose dehydrogenase. The flavin-binding glucose dehydrogenase of the invention has the following characteristics (1) and (4): (1) Molecular weight: the molecular weight of a polypeptide moiety in the enzyme is about 68 kDa as measured by SDS-polyacrylamide electrophoresis; (2) Km value: the Km value for D-glucose is about 15 mM or less; (3) Temperature stability: stable at a temperature of 55° C. or less; and (4) pH stability: stable at a pH range of 3.0 to 8.5.
    Type: Grant
    Filed: February 7, 2013
    Date of Patent: August 2, 2016
    Assignee: TOYOBO CO., LTD.
    Inventors: Yosuke Sumida, Rie Hirao, Yuu Utashima, Hiroshi Kawaminami, Hiroshi Aiba, Takahide Kishimoto, Shusaku Yanagidani
  • Patent number: 9394336
    Abstract: The present invention relates to purified peptidomimetic macrocycles. The invention additionally provides methods of preparing and using such macrocycles, for example in therapeutic applications.
    Type: Grant
    Filed: January 13, 2012
    Date of Patent: July 19, 2016
    Assignee: AILERON THERAPEUTICS, INC.
    Inventors: Huw M. Nash, Matthew Iadanza, Christopher Leitheiser, Noriyuki Kawahata
  • Patent number: 9260699
    Abstract: An object of the present invention is to provide a novel glucose dehydrogenase, a method for producing the glucose dehydrogenase, and applications of the glucose dehydrogenase. An isolated flavin-binding glucose dehydrogenase comprising a polypeptide having an amino acid sequence with 80% or more identity to the amino acid sequence of SEQ ID NO: 1, and having glucose dehydrogenase activity is provided.
    Type: Grant
    Filed: April 30, 2014
    Date of Patent: February 16, 2016
    Assignee: Toyobo Co., Ltd.
    Inventors: Yosuke Sumida, Hiroshi Aiba, Hiroshi Kawaminami, Rie Hirao, Yuu Utashima, Takahide Kishimoto, Shusaku Yanagidani
  • Patent number: 9206223
    Abstract: The present invention relates to purified peptidomimetic macrocycles. The invention additionally provides methods of preparing and using such macrocycles, for example in therapeutic applications.
    Type: Grant
    Filed: September 22, 2009
    Date of Patent: December 8, 2015
    Assignee: AILERON THERAPEUTICS, INC.
    Inventors: Huw M. Nash, Matthew Iadanza, Christopher Leitheiser, Noriyuki Kawahata
  • Patent number: 9181208
    Abstract: The present invention relates to a novel 3,6-anhydro-L-galactose dehydrogenase and to a novel compound produced therefrom. More specifically, provided is a 3,6-anhydro-L-galactose dehydrogenase which can produce 3,6-anhydrogalactonic acid of a novel type by metabolizing 3,6-anhydro-L-galactose.
    Type: Grant
    Filed: January 25, 2012
    Date of Patent: November 10, 2015
    Assignee: KOREA UNIVERSITY RESEARCH AND BUSINESS FOUNDATION
    Inventors: In-Geol Choi, Kyoung Heon Kim, Eun Ju Yun, Saeyoung Lee
  • Patent number: 9169468
    Abstract: The present invention provides methods and compositions suitable for use in the conversion of xylose to xylitol and xylulose, including nucleic acid constructs, recombinant fungal host cells, and related materials.
    Type: Grant
    Filed: January 14, 2014
    Date of Patent: October 27, 2015
    Assignee: Codexis, Inc.
    Inventors: Xiyun Zhang, Ryan C. Fong, Ezhilkani Subbian
  • Patent number: 9163220
    Abstract: The present invention relates to 3-hydroxypropionate dehydrogenase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
    Type: Grant
    Filed: April 1, 2014
    Date of Patent: October 20, 2015
    Assignees: Novozymes A/S, Novozymes, Inc.
    Inventors: Monica Tassone, Leonardo De Maria
  • Patent number: 9120844
    Abstract: A method for changing conformation of globular proteins is provided. The method controls the concentration of the globular proteins and the adsorption time of the globular proteins from the aqueous solution to the air/liquid interface, so that the main conformation of the globular proteins in a protein monolayer can be changed into ?-sheet or ?-helix. Meanwhile, the protein monolayer having the conformation of ?-sheet or ?-helix can be vertically deposited and transferred onto a substrate for various applications according to needs. The present invention can change three-dimensional structures of biological molecules and remain original functions thereof without additionally using any physical/chemical treatment to change the conformation of the globular proteins.
    Type: Grant
    Filed: May 24, 2012
    Date of Patent: September 1, 2015
    Assignee: NATIONAL CHENG KUNG UNIVERSITY
    Inventors: Yuh-lang Lee, Ke-hsuan Wang
  • Patent number: 9120841
    Abstract: The present invention provides an amphiphilic linear peptide and/or peptoid as well as a hydrogel that includes the amphiphilic linear peptide/peptoid.
    Type: Grant
    Filed: December 31, 2012
    Date of Patent: September 1, 2015
    Assignee: Agency for Science, Technology and Research
    Inventors: Charlotte Hauser, Yihua Loo
  • Publication number: 20150147783
    Abstract: The present invention relates to an isolated mutant eubacterium comprising at least one mutation resulting in a substitution of at least one amino acid in the beta-subunit of the RNA-polymerase encoded for by the rpoB-gene providing an altered production of a product of interest when said production of a product of interest is compared to the production of the same product in an isogenic wild type strain grown at identical conditions, wherein the substitution of at least one amino acid occurs at any of positions 469, 478, 482, 485, or 487 of SEQ ID NO:2, or at the equivalent positions in any eubacterial RNA-polymerase beta-subunit family member. Another aspect of the invention relates to a process for producing at least one product of interest in a mutant eubacterium and to a use of the mutant eubacterium according to the invention for producing at least one product of interest.
    Type: Application
    Filed: February 9, 2015
    Publication date: May 28, 2015
    Inventors: Steen Troels Joergensen, Jens Toenne Andersen, Niels Banke, Preben Nielsen
  • Patent number: 9040262
    Abstract: The present disclosure relates to non-naturally occurring polypeptides useful for preparing Ezetimibe, polynucleotides encoding the polypeptides, and methods of using the polypeptides.
    Type: Grant
    Filed: May 4, 2011
    Date of Patent: May 26, 2015
    Assignee: Codexis, Inc.
    Inventors: Michael Crowe, Oscar Alvizo, Behnaz Behrouzian, Yong Koy Bong, Steven J. Collier, Anupam Gohel, Jagadeesh Mavinahalli, Naga Modukuru, Emily Mundorff, Derek Smith, Shiwei Song, Wan Lin Yeo
  • Publication number: 20150141714
    Abstract: The disclosed invention provides methods and compositions for increasing terpenoid production, such as sesquiterpenoids, such as farnesene, in plant cells.
    Type: Application
    Filed: January 14, 2013
    Publication date: May 21, 2015
    Inventors: Joshua Blakeslee, Katrina Cornish, Oswald Crasta, Otto Folkerts, Dave Jessen, Ramesh Nair
  • Patent number: 9034887
    Abstract: A method of identifying an antifungal agent which targets a DHODH protein (alias PyrE, dihydroorotate dehydrogenase, EC: 1.3.99.11) of a fungus comprising contacting a candidate substance with a fungal DHODH protein and determining whether the candidate substance binds or modulates the DHODH protein, wherein binding or modulation indicates that the candidate substance is an antifungal agent. Specific examples concern Aspergillus fumigatus and Candida albicans DHODH proteins. DHODH inhibitors with a Quinazolinone core are also disclosed.
    Type: Grant
    Filed: May 1, 2009
    Date of Patent: May 19, 2015
    Assignee: F2G Limited
    Inventors: Jason David Oliver, John Leslie Thain, Michael John Bromley, Graham Edward Morris Sibley, Michael Birch
  • Publication number: 20150132806
    Abstract: The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme including the capability of reducing 5-((4S)-2-oxo-4-phenyl(1,3-oxazolidin-3-yl))-1-(4-fluorophenyl)pentane-1,5-dione to (4S)-3-[(5S)-5-(4-fluorophenyl)-5-hydroxypentanoyl]-4-phenyl-1,3-oxazolidin-2-one. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize the intermediate (4S)-3-[(5S)-5-(4-fluorophenyl)-5-hydroxypentanoyl]-4-phenyl-1,3-oxazolidin-2-one in a process for making Ezetimibe.
    Type: Application
    Filed: January 27, 2015
    Publication date: May 14, 2015
    Inventors: Emily Mundorff, Erik Jan De Vries
  • Patent number: 9029112
    Abstract: The present disclosure relates to engineered ketoreductase polypeptides and uses thereof for the preparation of ?-chloroalcohols from ?-chloroketones. Also provided are polynucleotides encoding the engineered ketoreductase polypeptides and host cells capable of expressing the engineered ketoreductase polypeptides.
    Type: Grant
    Filed: June 24, 2014
    Date of Patent: May 12, 2015
    Assignee: Codexis, Inc.
    Inventors: Yong Koy Bong, Michael Vogel, Steven J. Collier, Vesna Mitchell, Jagadeesh Mavinahalli
  • Publication number: 20150125925
    Abstract: The present invention provides consumer products comprising a serine protease variant, more specifically subtilisin variants produced there from. Specifically, the present invention provides consumer products comprising a serine protease variant, more specifically a subtilisin variant having one or more substitutions as compared to a reference serine protease. In addition, the present invention provides methods of making such compositions and methods of treating surfaces, particularly fabric surfaces comprising contacting a surface with an aqueous liquor comprising a serine protease variant, more specifically subtilisin variants and an adjunct material.
    Type: Application
    Filed: November 5, 2013
    Publication date: May 7, 2015
    Applicant: The Procter & Gamble Company
    Inventors: Philip Frank SOUTER, Euan John MAGENNIS, Glenn Steven WARD, Neelam S. AMIN, Katherine AUGUSTYN, Joshua R. BASLER, Luis Gustavo CASCAO-PEREIRA, Katherine D. COLLIER, Edward M. CONCAR, David A. ESTELL, James T. KELLIS, JR., Alexander PISARCHIK, Ayrookaran Joseph POULOSE, Jian YAO
  • Patent number: 9023608
    Abstract: The present invention relates to glucose dehydrogenase [NAD(P)+GDH] using nicotinamide adenine dinucleotide or nicotinamide adenine dinucleotide phosphate as a coenzyme, in which the thermal stability and/or the resistance to an organic solvent in the absence of sodium chloride are improved.
    Type: Grant
    Filed: March 30, 2012
    Date of Patent: May 5, 2015
    Assignee: Nipro Corporation
    Inventors: Masafumi Yao, Shido Kawase, Shin-ichi Yokobori, Akihiko Yamagishi
  • Publication number: 20150111280
    Abstract: The present invention provides a novel glucose dehydrogenase that has excellent substrate specificity, specific activity, thermal stability, and the like, and that is suitable for use in SMBG sensors. The present invention provides a purified polypeptide comprising an amino acid sequence having at least 80% identity to the sequence of SEQ ID NO: 1 and having glucose dehydrogenase activity.
    Type: Application
    Filed: December 29, 2014
    Publication date: April 23, 2015
    Inventors: Yosuke SUMIDA, Rie HIRAO, Yuu UTASHIMA, Hiroaki KITAZAWA, Hiroshi AIBA, Takahide KISHIMOTO, Shusaku YANAGIDANI
  • Publication number: 20150111269
    Abstract: Methods for the evolution of NADPH specific ketol-acid reductoisomerase enzymes to acquire NADH specificity are provided. Specific mutant ketol-acid reductoisomerase enzymes isolated from Pseudomonas that have undergone co-factor switching to utilize NADH are described.
    Type: Application
    Filed: December 23, 2014
    Publication date: April 23, 2015
    Inventors: Yougen LI, Der-Ing LIAO, Mark J. NELSON, Daniel P. OKEEFE
  • Patent number: 9005937
    Abstract: There are provided a novel ribitol dehydrogenase, a residue determining double coenzyme specificity, and a method for preparing L-ribulose using the same, and more particularly, to a ribitol dehydrogenase producing rare sugars, nucleic acid molecules encoding the same, a vector including the nucleic acid molecules, a transformant including the vector, a mutant of the ribitol dehydrogenase, and a method for preparing L-ribulose using the ribitol dehydrogenase. The ribitol dehydrogenase having double coenzyme specificity, which is derived from Zymomonas mobilis, can effectively be used for preparing high-priced rare sugars and an investigation of coenzyme specificity determinants for the ribitol dehydrogenase is applied for all of dedydrogenases as a based technique.
    Type: Grant
    Filed: January 27, 2012
    Date of Patent: April 14, 2015
    Assignee: Konkuk University Industrial Cooperation Corp.
    Inventors: Jung Kul Lee, Hee Jung Moon, Manish Tiwari, Tae Su Kim
  • Patent number: 9005952
    Abstract: The present invention relates to a microorganism producing L-methionine precursor, O-acetylhomoserine, and a method of producing L-methionine precursor using the microorganism.
    Type: Grant
    Filed: April 4, 2008
    Date of Patent: April 14, 2015
    Assignee: CJ Cheiljedang Corporation
    Inventors: Chul Ha Kim, So Young Kim, Young Uk Shin, Hye Won Um, Jin Sook Chang, Young Wook Cho, Han Jin Lee, In Kyung Heo, Chang Il Seo, Kwang Ho Na
  • Patent number: 8999691
    Abstract: Disclosed is a modified glucose dehydrogenases that has dramatically increased productivity in Escherichia coli and dramatically increased thermal stability, which is obtained by introducing specific amino acid mutations to glucose dehydrogenase derived from Botryotinia fuckeliana. Also disclosed is a modified glucose dehydrogenases that has dramatically increased productivity in E. coli and dramatically increased thermal stability, which is obtained by replacing two amino acid residues in glucose dehydrogenase of fungal origin with cysteine residues. The novel glucose dehydrogenase has a low reactivity to xylose.
    Type: Grant
    Filed: June 29, 2011
    Date of Patent: April 7, 2015
    Assignee: Ultizyme International Ltd.
    Inventors: Koji Sode, Kazushige Mori
  • Patent number: 8999140
    Abstract: Compositions, devices, kits and methods are disclosed for assaying glucose with a glucose oxidase mutant that has been modified at an amino acid residue involved in the active site. The glucose oxidase mutant has reduced oxidase activity while substantially maintaining its dehydrogenase activity.
    Type: Grant
    Filed: February 24, 2014
    Date of Patent: April 7, 2015
    Assignees: Roche Diagnostics Operations, Inc., Ultizyme International, Ltd.
    Inventors: Katsuhiro Kojima, Kazushige Mori, Sode Koji
  • Patent number: 8999661
    Abstract: The invention provides compounds and methods of their use in the detection of apoptosis and necrosis both in vitro and in vivo. Also provided are compounds and methods of their use in selective delivery of agents to cells undergoing apoptosis or necrosis. The compounds and methods are based on conjugates formed with a dehydrogenase such as lactate dehydrogenase, alcohol dehydrogenase, aldehyde dehydrogenase, and malate dehydrogenase. The compounds and methods are useful in the diagnosis and treatment of conditions characterized by apoptosis, including cancer, cardiac disease, neurologic disease including stroke, and autoimmunity. The compounds and methods offer distinct advantages over corresponding compounds and methods based on Annexin V. Also provided are methods for screening for compounds that modulate, i.e., inhibit or promote, apoptosis.
    Type: Grant
    Filed: July 29, 2009
    Date of Patent: April 7, 2015
    Assignee: Dana-Farber Cancer Institute, Inc.
    Inventors: Andrew Kung, Pallab Banerjee
  • Publication number: 20150083611
    Abstract: The present invention relates to a modified cellobiose dehydrogenase (CDH) or its functional flavodehydrogenase domain having glucose oxidation activity and a reduced maltose oxidation activity as compared to the unmodified CDH or its functional flavodehydrogenase domain, nucleic acids encoding said enzyme or domain, electrodes with said enzyme or domain and methods of producing and using the same.
    Type: Application
    Filed: March 6, 2013
    Publication date: March 26, 2015
    Inventors: Roland Ludwig, Christoph Sygmund, Wolfgang Harreither, Roman Kittl, Alfons Felice
  • Patent number: 8980605
    Abstract: The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds.
    Type: Grant
    Filed: March 11, 2013
    Date of Patent: March 17, 2015
    Assignee: Codexis, Inc.
    Inventors: Charlene Ching, John M. Gruber, Gjalt W. Huisman, Emily Mundorff, Lisa M. Newman