Acting On Choh Group As Donor (e.g., Glucose Oxidase, Lactate Dehydrogenase (1.1)) Patents (Class 435/190)
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Publication number: 20120244565Abstract: An object is to provide a novel enzyme that exhibits glucose dehydrogenase activity. Furthermore, another object is to provide a novel method pertaining to enzyme modification.Type: ApplicationFiled: November 22, 2010Publication date: September 27, 2012Applicant: Amano Enzyme Inc.Inventors: Kyoichi Nishio, Satoshi Koikeda
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Patent number: 8273563Abstract: A process is provided for producing peroxycarboxylic acids from carboxylic acid esters. More specifically, carboxylic acid esters are reacted with an inorganic peroxide, such as hydrogen peroxide, in the presence of an enzyme catalyst having perhydrolysis activity. The present perhydrolase catalysts are classified as members of the carbohydrate esterase family 7 (CE-7) based on the conserved structural features. Further, disinfectant formulations comprising the peracids produced by the processes described herein are provided.Type: GrantFiled: July 2, 2009Date of Patent: September 25, 2012Assignee: E. I. du Pont de Nemours and CompanyInventors: Robert DiCosimo, John Edward Gavagan, Mark Scott Payne, Frederick B. Cooling
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Patent number: 8273547Abstract: The present disclosure provides ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, method of using the engineered ketoreductase enzymes to synthesize a variety of chirally pure compounds, and the chirally pure compounds prepared therewith.Type: GrantFiled: December 23, 2010Date of Patent: September 25, 2012Assignee: Codexis, Inc.Inventors: Lorraine Joan Giver, Lisa Marie Newman, Emily Mundorff, Gjalt W. Huisman, Stephane J. Jenne, Jun Zhu, Behnaz Behrouzian, John H. Grate, James Lalonde
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Publication number: 20120231505Abstract: The present invention provides a method for producing a desired protein (such as a desired heterologous protein) comprising: (a) providing a host cell comprising a first recombinant gene encoding a protein comprising the sequence of a first chaperone protein, a second recombinant gene encoding a protein comprising the sequence of a second chaperone protein and a third gene, such as a third recombinant gene, encoding a desired protein (such as a desired heterologous protein), wherein the first and second chaperones are different; and (b) culturing the host cell in a culture medium to obtain expression of the first, second and third genes.Type: ApplicationFiled: May 17, 2012Publication date: September 13, 2012Applicant: NOVOZYMES BIOPHARMA DK A/SInventors: Christopher John Arthur Finnis, Darrell Sleep, Gillian Shuttleworth
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Publication number: 20120231515Abstract: From a bacterial strain isolated from an environmental sample, after enrichment in medium containing 1-butanol as the carbon source, a new enzyme with butanol dehydrogenase activity was identified. The enzyme can convert butyraldehyde to 1-butanol, isobutyraldehyde to isobutanol, as well as 2-butanone to 2-butanol and thus is useful for biosynthesis of butanol in recombinant microbial hosts producing these substrates. The encoding gene, named sadB, was isolated from the strain identified as an isolate of Achromobacter xylosoxidans.Type: ApplicationFiled: May 24, 2012Publication date: September 13, 2012Applicant: Butamax(TM) Advanced Biofuels LLCInventors: MICHAEL G. BRAMUCCI, Andrew C. Eliot, Lori Ann Maggio-Hall, Charles E. Nakamura
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Publication number: 20120231503Abstract: The present disclosure relates to a method for producing heterologous protein including: (c) providing a host cell comprising a 2 ?m-family plasmid, the plasmid comprising a gene encoding a protein comprising the sequence of a chaperone protein and a gene encoding a heterologous protein; (d) culturing the host cell in a culture medium under conditions that allow the expression of the gene encoding the chaperone protein and the gene encoding a heterologous protein; (e) purifying the thus expressed heterologous protein from the cultured host cell or the culture medium.Type: ApplicationFiled: May 17, 2012Publication date: September 13, 2012Applicant: NOVOZYMES BIOPHARMA DK A/SInventors: Darrell Sleep, Gillian Shuttleworth, Christopher John Arthur Finnis
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Patent number: 8257952Abstract: The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds.Type: GrantFiled: December 23, 2010Date of Patent: September 4, 2012Assignee: Codexis, Inc.Inventors: Onorato Campopiano, Emily Mundorff, Birthe Borup, Rama Voladri
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Patent number: 8252579Abstract: The present invention concerns a new method of preparation of a strain of evolved micro-organisms for the production of 1,2-propanediol by the metabolism of a simple carbon source, which method comprises the growth under selection pressure in an appropriate growth medium containing a simple carbon source of an initial bacterial strain that has undergone the deletion of the gene tpiA and the deletion of at least one gene involved in the conversion of methylglyoxal (propanal) into lactate, in order to cause, in said initial strain, the evolution of one or more genes involved in the biosynthesis pathway from DHAP to methylglyoxal and then to 1,2-propanediol towards evolved genes that possess an improved “1,2-propanediol synthase activity”, the resulting strain or strains of evolved micro-organisms possessing an improved “1,2-propanediol synthase activity” then being selected and isolated.Type: GrantFiled: January 12, 2005Date of Patent: August 28, 2012Assignee: Metabolic ExplorerInventors: Isabelle Meynial-Salles, Benjamin Gonzalez, Philippe Soucaille
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Patent number: 8247189Abstract: Provided is an enzyme that is further advantageous in terms of practical aspects when compared to publicly known enzymes for blood sugar sensors, and that can be used in a blood sugar level measuring reagent A flavin adenine dinucleotide-dependent glucose dehydrogenase that has amino acid sequence including a specific amino acid in an amino acid sequence shown in SEQ ID NO:2 or an amino acid sequence that has 60% homology therewith, and that has an improved temperature dependency.Type: GrantFiled: March 15, 2012Date of Patent: August 21, 2012Assignee: Toyo Boseki Kabushiki KaishaInventors: Hiroshi Kawaminami, Masao Kitabayashi, Yoshiaki Nishiya
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Patent number: 8236551Abstract: The present application provides genetically modified fungal organisms that produce enzyme mixtures exhibiting enhanced hydrolysis of cellulosic material to glucose, enzyme mixtures produced by the genetically modified fungal organisms, and processes for producing glucose from cellulose using such enzyme mixtures.Type: GrantFiled: November 1, 2011Date of Patent: August 7, 2012Assignee: Codexis, Inc.Inventors: Ish Kumar Dhawan, Dipnath Baidyaroy, Andrew Shaw, Oleh Tanchak, Christopher Hill, Chengsong Liu, Amala Chokshi, Brian R. Scott
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Publication number: 20120196342Abstract: The present invention relates to methods of reducing the toxicity of lignocellulosic hydrolysates which comprise one or more inhibitors. One method reduces the amount of furfural inhibitor leading to a more effective process. Another method reduces the amount of xylitol produced during the fermentation of xylose present in lignocellulosic hydrolysates. Naturally occurring aldo/keto reductase enzymes, as well as, enzymes produced by recombinant cells or by selective adaptation may be employed.Type: ApplicationFiled: February 1, 2011Publication date: August 2, 2012Inventors: Rachel Ruizhen Chen, Manoj Agrawal
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Publication number: 20120190086Abstract: The present disclosure relates to engineered ketoreductase polypeptides and uses thereof for the preparation of ? chloroalcohols from ? chloroketones. Also provided are polynucleotides encoding the engineered ketoreductase polypeptides and host cells capable of expressing the engineered ketoreductase polypeptides.Type: ApplicationFiled: June 22, 2010Publication date: July 26, 2012Applicant: CODEXIS, INC.Inventors: Yong Koy Bong, Michael Vogel, Steven J. Collier, Vesna Mitchell, Jagadeesh Mavinahalli
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Publication number: 20120178142Abstract: The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds.Type: ApplicationFiled: November 7, 2011Publication date: July 12, 2012Applicant: CODEXIS, INC.Inventors: Charlene Ching, John M. Gruber, Gjalt W. Huisman, Emily Mundorff, Lisa M. Newman
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Publication number: 20120178637Abstract: Methods for classifying a test sample as indicative of Alzheimer's disease use protein and peptide biomarkers that are differentially expressed in the cerebral spinal fluid (CSF) of subjects with Alzheimer's disease relative to age-matched controls. The methods also use protein and peptide signatures indicative of Alzheimer's disease. Microarrays and kits for detecting the protein and peptide biomarkers in CSF samples can be used to classify Alzheimer's disease state from test samples.Type: ApplicationFiled: July 7, 2010Publication date: July 12, 2012Applicant: ABBOTT LABORATORIESInventors: Viswanath Devanarayan, Melanie Joy Patterson, Jeffrey F. Waring, David Witte
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Publication number: 20120178115Abstract: The present invention relates to novel mutants of PQQ s-GDH containing an amino acid substitution in position 428 of the protein sequence of the wild type PQQ s-GDH of Acinetobacter calcoaceticus (SEQ.ID. NO:2). The invention also relates to the use of said PQQ s-GDH mutants for the development of glucose electrodes of interest in the assay of glucose, in particular of blood glucose in diabetic subjects, and for implementing biofuel cells that utilize glucose as fuel.Type: ApplicationFiled: July 20, 2010Publication date: July 12, 2012Inventors: Nicolas Mano, Claire Stines-Chaumeil, Fabien Durand
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Publication number: 20120174255Abstract: This disclosure concerns specific naturally-occurring mutant maize cad2 genes, which altered genes contribute to the bm1 maize phenotype in particular maize lines. In some embodiments, compositions and methods are provided that utilize a nucleic acid molecule comprising a mutant cad2 gene, and/or markers linked to a mutant cad2gene.Type: ApplicationFiled: January 3, 2012Publication date: July 5, 2012Applicant: AGRIGENETICS, INC.Inventors: Wei Chen, Nathan J. VanOpdorp, Siva P. Kumpatla, Peizhong Zheng, Peter D. Friedemann, Thomas W. Greene, Dennis Fitzl
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Publication number: 20120171708Abstract: Provided is an enzyme that is further advantageous in terms of practical aspects when compared to publicly known enzymes for blood sugar sensors, and that can be used in a blood sugar level measuring reagent. A flavin adenine dinucleotide-dependent glucose dehydrogenase that has amino acid sequence including a specific amino acid in an amino acid sequence shown in SEQ ID NO: 2 or an amino acid sequence that has a 60% homology therewith, and that has an improved temperature dependency.Type: ApplicationFiled: March 15, 2012Publication date: July 5, 2012Applicant: TOYO BOSEKI KABUSHIKI KAISHAInventors: Hiroshi KAWAMINAMI, Masao KITABAYASHI, Yoshiaki NISHIYA
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Patent number: 8211682Abstract: This invention relates to a composite material that comprises a support member that has a plurality of pores extending through the support member and, located in the pores of the support member, and filling the pores of the support member, a macroporous cross-linked gel. The invention also relates to a process for preparing the composite material described above, and to its use. The composite material is suitable, for example, for separation of substances, for example by filtration or adsorption, including chromatography, for use as a support in synthesis or for use as a support for cell growth.Type: GrantFiled: October 3, 2008Date of Patent: July 3, 2012Assignee: Natrix Separations Inc.Inventors: Ronald F. Childs, Carlos Filipe, Raja Ghosh, Alicja M. Mika, Jinsheng Zhou, Elena N. Komkova, Marcus Y. Kim, Tapan K. Dey
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Publication number: 20120164696Abstract: The invention relates to recombinant expression of a variant form of a fungal C1 strain ?-glucosidase. The invention also relates to the generation of fermentable sugars from biomass and the production of biofuels by fermentation of the sugars using genetically modified organisms expressing the ?-glucosidase variant. The invention provides methods for producing a fermentable sugar, such as glucose, from cellobiose by contacting cellobiose with a recombinant ?-glucosidase variant protein, such as a variant protein secreted by a recombinant host cell into culture medium. Methods of the invention may be used for conversion of a biomass substrate to a fermentable sugar, and ultimately to ethanol or other biofuel.Type: ApplicationFiled: March 9, 2012Publication date: June 28, 2012Applicant: Codexis, Inc.Inventors: Jie Yang, Xiyun Zhang, Attila Andor
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Publication number: 20120135487Abstract: The present invention concerns a method for the production of 1,2-propanediol, comprising culturing a microorganism modified for an improved production of 1,2-propanediol in an appropriate culture medium and recovery of the 1,2-propanediol which may be further purified wherein the microorganism expresses a glycerol dehydrogenase (GlyDH) enzyme the inhibition of which activity by NAD+ and/or its substrate and/or its product is reduced. The present invention also relates to a mutant glycerol dehydrogenase (GlyDH) comprising at least one amino acid residue in the protein sequence of the parent enzyme replaced by a different amino acid residue at the same position wherein the mutant enzyme has retained more than 50% of the glycerol dehydrogenase activity of the parent enzyme and the glycerol dehydrogenase activity of the mutant GlyDH is less inhibited by NAD+ and/or by its substrate as compared to the parent enzyme and/or by its product as compared to the parent enzyme.Type: ApplicationFiled: July 30, 2010Publication date: May 31, 2012Applicant: METABOLIC EXPLORERInventors: Francois Voelker, Laurence Dumon-Seignovert, Isabelle Meynial-Salles, Philippe Soucaille
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Patent number: 8178090Abstract: Use of a preparation as an antimicrobial agent or as an anti-oxidant; wherein said preparation comprises a polypeptide having hexose oxidase activity, the polypeptide being in a substantially pure form, and said polypeptide comprises at least one amino acid sequence selected from the group consisting of (i) (SEQ?ID?NO:?1) Tyr-Glu-Pro-Tyr-Gly-Gly-Val-Pro (ii) (SEQ?ID?NO:?2) Ala-Ile-Ile-Asn-Val-Thr-Gly-Leu-Val-Glu-Ser-Gly- Tyr-Asp-X-X-X-Gly-Tyr-X-Val-Ser-Ser- (iii) (SEQ?ID?NO:?3) Asp-Leu-Pro-Met-Ser-Pro-Arg-Gly-Val-Ile-Ala-Ser- Asn-Leu-X-Phe- (iv) (SEQ?ID?NO:?4) Asp-Ser-Glu-Gly-Asn-Asp-Gly-Glu-Leu-Phe-X-Ala-His- Thr (v) (SEQ?ID?NO:?5) Tyr-Tyr-Phe-Lys (vi) (SEQ?ID?NO:?6) Asp-Pro-Gly-Tyr-Ile-Val-Ile-Asp-Val-Asn-Ala-Gly- Thr-X-Asp (vii) (SEQ?ID?NO:?7) Leu-Gln-Tyr-Gln-Thr-Tyr-Trp-Gln-Glu-Glu-Asp (viii) (SEQ?ID?NO:?8) X-Ile-Arg-Asp-Phe-Tyr-Glu-GluType: GrantFiled: March 3, 2009Date of Patent: May 15, 2012Assignee: Danisco A/SInventors: Peter Stougaard, Ole Cai Hansen, Charlotte Horsmans Poulsen, Karsten Matthias Kragh
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Publication number: 20120115197Abstract: Methods for the evolution of NADPH binding ketol-acid reductoisomerase enzymes to acquire NADH binding functionality are provided. Specific mutant ketol-acid reductoisomerase enzymes isolated from Pseudomonas that have undergone co-factor switching to bind NADH are described.Type: ApplicationFiled: November 15, 2011Publication date: May 10, 2012Inventors: YOUGEN LI, DER-ING LIAO, MARK J. NELSON, DANIEL P. OKEEFE
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Patent number: 8173402Abstract: The present invention relates to novel yeast strains, methods and genetic constructs for their preparation, and their use for the synthesis or modification of steroidal compounds. More particularly, the invention describes strains having a reduced 20?HSD type activity, in particular by modifying the GCY1 and/or YPR1 genes. The yeast strains of the invention make it possible to improve the efficiency of the synthesis or to increase the selectivity or the yields of the method, as well as the quality of the final product. The strains, methods and compounds of the invention are useful in the search for, the development and the production of products with therapeutic or prophylactic activity, in humans or animals, in particular of steroidal derivatives.Type: GrantFiled: January 14, 2011Date of Patent: May 8, 2012Assignee: Aventis Pharma S.A.Inventors: Bruno Dumas, Gilles Cauet, Eric Degryse, Tilman Achstetter
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Publication number: 20120107903Abstract: A mutant glucose dehydrogenase having an amino acid sequence at least 80% identical to SEQ ID NO:3 and having glucose dehydrogenase activity, wherein amino acid residues corresponding to positions 326, 365 and 472 of said amino acid sequence are replaced with glutamine, tyrosine and tyrosine, respectively, and wherein said mutant glucose dehydrogenase shows an improved substrate specificity to glucose and a reduced reactivity to disaccharides.Type: ApplicationFiled: October 26, 2011Publication date: May 3, 2012Applicants: BIOENGINEERING LABORATORIES, LLC, ARKRAY, Inc.Inventors: Koji Sode, Katsuhiro Kojima
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Publication number: 20120094358Abstract: Biomass (e.g., plant biomass, animal biomass, microbial, and municipal waste biomass) is processed to produce useful products, such as food products and amino acids.Type: ApplicationFiled: December 6, 2011Publication date: April 19, 2012Applicant: XYLECO, INC.Inventor: Marshall Medoff
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Patent number: 8153392Abstract: A method of detecting, diagnosing and prognosticating atherosclerosis by measuring the activities of ?6 and ?5 desaturases is described. It is suggested that enhancing the activities of ?6 and ?5 desaturases results in an increase in the plasma, leukocyte, platelet and endothelial cell levels of ?-linolenic, dihomo-?-linolenic, arachidonic, stearidonic, 20:4 ?-3, eicosapentaneoic and docosahexaenoic acids and PGE1 (prostaglandin E1), prostacyclin (PGI2), prostaglandin I3 (PGI3), lipoxins, resolvins, protectins, nitric oxide, and nitrolipids that prevent, arrest and reverse atherosclerosis. The invention is also directed to the delivery of proteins, peptides, lipids, lipoproteins, glycolipids, statins and troglitazones and their derivatives, and other compounds (synthetic or natural), cDNA clones and genes of ?6 and ?5 desaturases to enhance the activities of ?6 and ?5 desaturases in vivo to prevent, arrest, reverse and treat atherosclerosis.Type: GrantFiled: March 28, 2008Date of Patent: April 10, 2012Inventors: Appa Rao Allam, Undurti Narasimha Das
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Publication number: 20120083024Abstract: The subject invention concerns polynucleotides encoding a plant 2-phenylethanol dehydrogenase enzyme. In one embodiment, the polynucleotide encodes a tomato 2-phenylethanol dehydrogenase. The subject invention also concerns polynucleotides encoding a plant phenylalanine decarboxylase enzyme. In one embodiment, the polynucleotide encodes a tomato phenylalanine decarboxylase. The subject invention also concerns 2-phenylethanol dehydrogenase polypeptides and phenylalanine decarboxylase polypeptides encoded by polynucleotides of the present invention. The subject invention also concerns methods for providing a plant with an increased flavor and aroma volatile. Plants can be transformed with one or more polynucleotide of the present invention. The subject invention also concerns these transformed plant cells, plant tissue, and plants and transgenic progeny thereof.Type: ApplicationFiled: October 1, 2004Publication date: April 5, 2012Inventors: Harry J. Klee, Denise Tieman
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Publication number: 20120083426Abstract: A method is described for releasing a soluble or membrane associated intracellular protein of interest (POI) comprising the steps of: providing a cell comprising a soluble or membrane associated intracellular POI; contacting the cell with a membrane extracting composition; and causing the POI to be released from the cell under conditions sufficient for the specific release of the POI and in a soluble form.Type: ApplicationFiled: July 21, 2011Publication date: April 5, 2012Inventors: Claus Lindvald JOHANSEN, Soren Kjaerulff, Susan Mampusta Madrid, Henrik Pedersen, Charlotte Horsmans Poulsen, Masoud Rajabi Zargahi
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Patent number: 8143038Abstract: A thermophilic micro-organism comprising a modification that increases amylase expression and starch hydrolysis compared to wild-type, wherein the modification is insertion of a heterologous amylase gene.Type: GrantFiled: July 26, 2011Date of Patent: March 27, 2012Assignee: TMO Renewables LimitedInventors: Anthony Atkinson, Roger Cripps, Kirstin Eley, Brian Rudd, Martin Todd
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Publication number: 20120070871Abstract: Highly productive D-lactic acid fermentation uses a transformant obtained by introducing into a host cell a polynucleotide encoding a polypeptide according to any one of the following (A) to (C) in such a manner that the polypeptide is expressed, which polypeptide has a D-lactate dehydrogenase activity higher than those of conventional polypeptides: (A) a polypeptide having the amino acid sequence shown in SEQ ID NO:1 or 2; (B) a polypeptide having the same amino acid sequence as shown in SEQ ID NO:1 or 2 except that one or several amino acids are substituted, deleted, inserted and/or added, which polypeptide has a D-lactate dehydrogenase activity; and (C) a polypeptide having an amino acid sequence which has a sequence identity of not less than 80% to the amino acid sequence shown in SEQ ID NO:1 or 2, which polypeptide has a D-lactate dehydrogenase activity.Type: ApplicationFiled: June 2, 2010Publication date: March 22, 2012Applicant: Toray Industries, Inc.Inventors: Kenji Sawai, Kazumi Suda, Hideki Sawai, Katsushige Yamada, Junya Yamagishi
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Patent number: 8137940Abstract: The present invention relates to microorganisms genetically engineered to increase yield and/or efficiency of biomass production from a carbon source, such as e.g. glucose. Processes for generating such microorganisms are also provided by the present invention. The invention also relates to polynucleotide sequences comprising genes that encode proteins that are involved in the bioconversion of a carbon source such as e.g. glucose into biomass. The invention also features polynucleotides comprising the full-length polynucleotide sequences of the novel genes and fragments thereof, the novel polypeptides encoded by the polynucleotides and fragments thereof, as well as their functional equivalents. Also included are processes of using the polynucleotides and modified polynucleotide sequences to transform host microorganisms leading to a microorganism with reduced carbon source diversion, i.e. higher yield and/or efficiency of biomass production from a carbon source such as e.g. glucose.Type: GrantFiled: September 7, 2006Date of Patent: March 20, 2012Assignee: DSM IP Assets B.V.Inventor: Masako Shinjoh
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Patent number: 8137941Abstract: A chondroitin polymerase having such properties that it transfers GlcUA and GalNAc alternately to a non-reduced terminal of a sugar chain from a GlcUA donor and a GalNAc donor, respectively, and the like; and a process for producing the chondroitin polymerase.Type: GrantFiled: August 10, 2009Date of Patent: March 20, 2012Assignee: Seikagaku CorporationInventors: Toshio Ninomiya, Nobuo Sugiura, Koji Kimata
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Publication number: 20120064602Abstract: Described are compositions and methods that involve using bleaching enzymes in dish detergents. In some preferred embodiments, the bleaching enzyme comprises at least one laccase, while in some alternative preferred embodiments, the bleaching enzyme comprises at least one glucose oxidase suitable for use in dish detergents. In some additional preferred embodiments, the dish detergents are machine dish detergents.Type: ApplicationFiled: May 20, 2010Publication date: March 15, 2012Inventors: Pieter Augustinus, Maria Petra Van Deurzen, Quinta Mildred Westdijk-Patist
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Patent number: 8124389Abstract: The present disclosure provides a crystal structure of aldehyde dehydrogenase (ALDH) with a modulator of ALDH bound thereto. The present disclosure provides a computer readable medium comprising atomic coordinates for an ALDH polypeptide and a modulator bound to a site within the polypeptide. A method is also provided. In general terms, the method comprises computationally identifying a compound that binds to an ALDH polypeptide, using the atomic coordinates.Type: GrantFiled: May 4, 2009Date of Patent: February 28, 2012Assignees: The Board of Trustees of the Leland Stanford Junior University, Indiana University Research and Technology CorporationInventors: Che-Hong Chen, Daria Mochly-Rosen, Thomas D. Hurley
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Publication number: 20120043204Abstract: The invention provides an enzyme ink useful in test strips that provides a test strip bias, at the low and high glucose ends, falling within a desired target range. The ink of the invention permits an improved method for the production of single calibration code strip lots with good yields.Type: ApplicationFiled: August 23, 2010Publication date: February 23, 2012Applicant: LifeScan Scotland Ltd.Inventors: Gary YOUNG, Michael O'Connell, Ian McArthur, Alan McNeilage, Nick Phippen, Manuel Alvarez-Icaza
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Publication number: 20120040387Abstract: The present invention provides a method for measuring the concentration of an analyte in a test solution wherein the analyte is mevalonic acid and/or 3-hydroxymethylglutaryl coenzyme A, comprising the following steps (p) and (q): (p) a step of allowing an enzyme that catalyzes a reaction represented by Reaction Formula 1 and an enzyme that catalyzes a reaction represented by Reaction Formula 2 to act on a test solution containing mevalonic acid and/or 3-hydroxymethylglutaryl coenzyme A in the presence of a hydrogen acceptor X, a hydrogen donor Y, and coenzyme A; and (q) a step of measuring an amount of: a reduced hydrogen acceptor X that is produced; or an oxidized hydrogen donor Y that is produced; or a hydrogen acceptor X that is decreased; or a hydrogen donor Y that is decreased, wherein the hydrogen donor Y and the reduced hydrogen acceptor X are not the same.Type: ApplicationFiled: January 19, 2009Publication date: February 16, 2012Applicant: ASAHI KASEI PHARMA CORPORATIONInventor: Takeshi Matsuoka
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Patent number: 8114606Abstract: This invention provides antibodies immunologically specific for human ARL-1 (also referred to AKR1B10), a species of the aldo-keto reductase superfamily of proteins. The invention also provides methods of making and methods of using said antibodies.Type: GrantFiled: February 15, 2008Date of Patent: February 14, 2012Assignee: The Board of Trustees of Southern Illinois UniversityInventor: Deliang Cao
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Patent number: 8114638Abstract: Stable and active arabinitol dehydrogenases (LAD) from Neurospora crassa and mutants thereof are disclosed. Arabinitol dehydrogenases are useful in the production of xylitol and ethanol from an arabinose containing substrate. Recombinant and heterologously expressed arabinitol dehydrogenases are useful in converting biomass into biofuels and other industrial food products.Type: GrantFiled: January 4, 2011Date of Patent: February 14, 2012Assignees: The Board of Trustees of the University of Illinois, Biotechnology Research and Development CorporationInventors: Huimin Zhao, Ryan Sullivan
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Publication number: 20120028911Abstract: The present technology relates to isolated MUC17, Muc3 or MUC3 derived polypeptides and polynucleotides encoding the same. The MUC17, Muc3 or MUC3 derived polypeptides and polynucleotides can be used to treat gastrointestinal tract diseases and disorders. Also described herein are pharmaceutical compositions comprising MUC17, Muc3 or MUC3 derived polypeptides and/or polynucleotides encoding the same alone or in combination along with a pharmaceutically acceptable carrier, diluent or excipient for the treatment of gastrointestinal disorders and diseases, including, irritable bowel disease and its associated colitides, for example, Crohn's Disease.Type: ApplicationFiled: April 29, 2011Publication date: February 2, 2012Applicant: MuciMed, Inc.Inventors: Ronald J. Shebuski, Samuel B. Ho, Laurie Shekels, Robert L. Heinrikson
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Publication number: 20120021468Abstract: The invention provides methods and compositions for the production of L-ribitol and other rare sugars using a mannitol-1-dehydrogenase or a polyol-1-dehydrogenase.Type: ApplicationFiled: August 20, 2009Publication date: January 26, 2012Applicant: ZUCHEM, INC.Inventors: Ryan Woodyer, Francis Michael Racine
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Patent number: 8101374Abstract: It is an object of the present invention to provide a means for measuring a concentration of various components rapidly with high accuracy and high sensitivity. The present invention is to provide an oxidative chromogenic compound or salt thereof represented by the following chemical formula (1): wherein R1 and R2 are each independently a linear or branched alkyl group having 1 to 5 carbon atoms; R3, R4, R5, R6 and R7 are each independently a hydrogen atom, a carboxyl group or a sulfonic acid group, or salt thereof, provided that at least one of R3 to R7 is a carboxyl group or a sulfonic acid group, or salt thereof, and the others are hydrogen atoms, and the production method thereof, and the reagent composition and test instrument using the same.Type: GrantFiled: March 6, 2008Date of Patent: January 24, 2012Assignee: Terumo Kabushiki KaishaInventors: Tetsuro Kawanishi, Yuzuru Saito, Takako Ariga
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Patent number: 8097460Abstract: The present invention relates to the production of ethanol as a product of bacterial fermentation. In particular this invention relates to a novel method of gene inactivation and gene expression based upon homologous recombination. The method is particularly useful in connection with species of Bacillus such as B. stereothermophilus, B. calvodelox, B. caldotenax, B. thermoglucosidasius, B. coagulans, B. licheniformis, B. thermodenitrificans, and B. caldolyticus.Type: GrantFiled: February 24, 2009Date of Patent: January 17, 2012Assignee: Elsworth Biotechnology LimitedInventors: Muhammed Javed, Fiona Cusdin, Paul Milner, Edward Green
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Publication number: 20120009298Abstract: A noodle having improved physical properties and improved taste may be obtained with an enzyme preparation for modifying a noodle, which contains ?-glucosidase and glucose oxidase.Type: ApplicationFiled: July 12, 2011Publication date: January 12, 2012Applicant: AJINOMOTO CO., INC.Inventor: Noriaki YAMADA
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Patent number: 8093346Abstract: Provided is a method for efficiently producing a 3-amino-4-hydroxybenzoic acid-type compound by culturing a coryneform bacterium that has a gene encoding a mutated aspartokinase not subject to feedback inhibition, and that is transformed with a recombinant vector containing a DNA encoding a protein having an activity to form 3-amino-4-hydroxybenzoic acid from dihydroxyacetone phosphate and aspartate semialdehyde.Type: GrantFiled: January 3, 2011Date of Patent: January 10, 2012Assignee: Ajinomoto Co., Inc.Inventors: Mototaka Suzuki, Keiichi Yokoyama, Yoshimi Kikuchi
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Patent number: 8088610Abstract: The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds.Type: GrantFiled: September 29, 2008Date of Patent: January 3, 2012Assignee: Codexis, Inc.Inventors: Jack Liang, Birthe Borup, Vesna Mitchell, Emily Mundorff, James LaLonde, Gjalt W. Huisman
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Publication number: 20110318810Abstract: A flavin-binding glucose dehydrogenase with a high substrate specificity for D-glucose. The flavin-binding glucose dehydrogenase which is derived from a microorganism belonging to the genus Mucor. The flavin-binding glucose dehydrogenase has a low reactivity for maltose, D-galactose and D-xylose compared to its reactivity for D-glucose, and therefore is relatively unaffected by these saccharide compounds. The flavin-binding glucose dehydrogenase is also relatively unaffected by dissolved oxygen, and allows accurate measurement of glucose amounts even in the presence of saccharide compounds other than glucose in samples.Type: ApplicationFiled: April 19, 2010Publication date: December 29, 2011Applicant: KIKKOMAN CORPORATIONInventors: Ryoko Tajima, Atsushi Ichiyanagi, Keiichi Ichikawa, Taro Yoshimura
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Publication number: 20110321197Abstract: A method for producing a plant with increased yield as compared to a corresponding wild type plant whereby the method comprises at least the following step: increasing or generating in a plant or a part thereof one or more activities selected from the group consisting of 17.6 kDa class I heat shock protein, 26.Type: ApplicationFiled: October 2, 2009Publication date: December 29, 2011Applicant: BASF Plant Science GmbHInventors: Hardy Schön, Oliver Thimm, Gerhard Ritte, Oliver Bläsing, Koen Bruynseels, Yves Hatzfeld, Valerie Frankard, Ana Isabel Sanz Molinero, Christophe Reuzeau, Steven Vandenabeele
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Publication number: 20110318809Abstract: The present invention relates to a method of screening placental proteins responsible for pathophysiology of preeclampsia, and a marker for early diagnosis and prediction of preeclampsia. In accordance with one aspect of the present invention, there is provided a method of screening placental proteins responsible for pathophysiology of preeclampsia by 2D E-proteomics analysis, comprising: isolating placental proteins from a placental tissue; separating the isolated proteins two-dimensionally through 2D electrophoresis; and comparing and analyzing the separated proteins based on scanned gel images and differences in the images between normal placental proteins and preeclamptic placental proteins, wherein the comparison and analysis of the placental proteins based on the scanned gel images and differences in the images are accomplished by selecting proteins with differences of 140% or more between two placentas.Type: ApplicationFiled: June 7, 2011Publication date: December 29, 2011Inventors: Won Sun Park, Na Ri Kim, Jin Han
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Patent number: 8080397Abstract: A bioengineered synthesis scheme for the production of quinic acid from a carbon source is provided. Methods of producing quinic acid from a carbon source based on the synthesis scheme as well as conversion of quinic acid to hydroquinone are also provided.Type: GrantFiled: November 20, 2009Date of Patent: December 20, 2011Assignee: Board of Trustees Operating Michigan State UniversityInventors: John W. Frost, Karen M. Frost
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Publication number: 20110306076Abstract: The present invention relates to cellobiose dehydrogenases (CDH) having glucose oxidation activity at a pH of 7.4 or above, modifications to modify the pH dependency of the enzymes activity, uses for these CDHs, in particular electrode sensors and electrochemical cells.Type: ApplicationFiled: February 26, 2010Publication date: December 15, 2011Inventors: Roland Ludwig, Dietmar Haltrich, Wolfgang Harreither, Lo Gorton