Alpha-amylase, Microbial Source Patents (Class 435/202)
-
Publication number: 20140315243Abstract: The present invention relates to processes for producing fermentation products from starch-containing material, wherein an alpha-amylase, a thermostable protease, and optionally a carbohydrate-source generating enzyme and/or pullulanase, are present and/or added during liquefaction. The invention also relates to compositions suitable for use in a process of the invention.Type: ApplicationFiled: November 30, 2012Publication date: October 23, 2014Inventors: Randall Deinhammer, Joyce Craig, Tomoko Matsui, Shinobu Takagi, Suzanne Clark, John Matthews, Anne Glud Hjulmand, Chee-Leong Soong
-
Patent number: 8859255Abstract: The invention relates to a variant of a parent Termamyl-like alpha-amylase, which variant exhibits altered properties, in particular reduced capability of cleaving a substrate close to the branching point, and improved substrate specificity and/or improved specific activity relative to the parent alpha-amylase.Type: GrantFiled: March 12, 2013Date of Patent: October 14, 2014Assignee: Novozymes A/SInventors: Carsten Andersen, Christel Thea Jorgensen, Henrik Bisgaard-Frantzen, Allan Svendsen, Soren Kjaerulff
-
Patent number: 8852912Abstract: Described are compositions and methods relating to variant alpha-amylases having altered biochemical properties and advantageous performance characteristics as compared to a reference alpha-amylase. The variants are suitable for use in various industrial applications such as starch conversion, ethanol production, laundry, dishwashing, pulp and paper production, textile desizing, and/or sweetener production.Type: GrantFiled: April 1, 2010Date of Patent: October 7, 2014Assignee: Danisco US Inc.Inventors: David A. Estell, Brian E. Jones, Marc Kolkman, Christian D. Adams, Edward M. Concar
-
Publication number: 20140287477Abstract: Disclosed are compositions and methods relating to variant maltohexaose-forming alpha-amylases. The variant alpha-amylases are useful, for example, for starch liquefaction and saccharification, for cleaning starchy stains in laundry, dishwashing, and other applications, for textile processing (e.g., desizing), in animal feed for improving digestibility, and for baking and brewing.Type: ApplicationFiled: October 26, 2012Publication date: September 25, 2014Applicant: DANISCO US INC.Inventors: Luis G. Cascao-Pereira, David A. Estell, Marc Kolkman, Harm Mulder
-
Publication number: 20140273135Abstract: The present invention relates to processes for producing fermentation products from starch-containing material, wherein a bacterial alpha-amylase, a raw starch hydrolyzing alpha-amylase and a carbohydrate-source generating enzyme are present and/or added during liquefaction. The invention also relates to compositions suitable for use in processes of the invention.Type: ApplicationFiled: October 9, 2012Publication date: September 18, 2014Inventors: Randall Deinhammer, Anne Glud Hjulmand, Joyce Craig, Guillermo Coward-Kelly, Tomoko Matsui, Shinobu Takagi, Suzanne Clark, John Matthews
-
Patent number: 8828460Abstract: The inventors have modified the amino acid sequence of a maltogenic alpha-amylase to obtain variants with improved properties, based on the three-dimensional structure of the maltogenic alpha-amylase Novamyl. The variants have altered physicochemical properties., e.g. an altered pH optimum, improved thermostability, increased specific activity, an altered cleavage pattern or an increased ability to reduce retrogradation of starch or staling of bread.Type: GrantFiled: August 27, 2013Date of Patent: September 9, 2014Assignee: Novozymes A/SInventors: Joel Cherry, Allan Svendsen, Carsten Andersen, Lars Beier, Torben Peter Frandsen
-
Publication number: 20140248397Abstract: A variant polypeptide having alpha-amylase activity, wherein the variant has an amino acid sequence which, when aligned with the alpha-amylase comprising the sequence set out in SEQ ID NO: 2, comprises at least one substitution of an amino acid residue corresponding to any of amino acids 4, 6, 13, 14, 15, 16, 20, 45, 47, 51, 54, 61, 68, 69, 70, 71, 72, 73, 74, 75, 77, 78, 80, 82, 87, 88, 94, 95, 100, 103, 104, 117, 124, 125, 126, 128, 130, 133, 134, 136, 143, 144, 146, 168, 174, 177, 178, 183, 186, 188, 189, 190, 194, 195, 199, 200, 201, 204, 207, 210, 214, 217, 219, 222, 225, 227, 233, 234, 235, 236, 240, 251, 252, 254, 258, 259, 260, 261, 262, 263, 264, 266, 267, 269, 271, 273, 281, 282, 283, 284, 286, 288, 299, 322, 323, 325, 327, 328, 331, 334, 350, 356, 358, 367, 370, 371, 374, 377, 378, 388, 391, 414, 421, 422, 445, 450, 467, 488, 505, 536, 548, 583, 588, 603, 637, 648, 651, 652, 660, 676, 677, said positions being defined with reference to SEQ ID NO: 2 and wherein the variant has one or more altered prType: ApplicationFiled: March 15, 2013Publication date: September 4, 2014Applicant: DSM IP ASSETS B.V.Inventors: Rene Marcel DE JONG, Hanna Elisabet ABBAS
-
Publication number: 20140248685Abstract: The invention relates to a variant of a parent Termamyl-like ?-amylase, which exhibits an alteration in at least one of the following properties relative to said parent ?-amylase: i) improved pH stability at a pH from 8 to 10.5; and/or ii) improved Ca2+ stability at pH 8 to 10.5, and/or iii) increased specific activity at temperatures from 10 to 60° C.Type: ApplicationFiled: January 24, 2014Publication date: September 4, 2014Applicant: NOVOZYMES A/SInventors: Torben Vedel Borchert, Allan Svendsen, Carsten Andersen, Bjarne Nielsen, Torben Lauesgaard Nissen, Soren Kjaerulff
-
Publication number: 20140242667Abstract: The present invention relates to variants of a parent ?-amylase, which parent ?-amylase (i) has an amino acid sequence selected from the amino acid sequences shown in SEQ ID No. 1, SEQ ID No. 2, SEQ ID No. 3, and SEQ ID No.Type: ApplicationFiled: April 7, 2014Publication date: August 28, 2014Applicant: NOVOZYMES A/SInventors: Henrik Bisgaard-Frantzen, Allan Svendsen, Torben Vedel Borchert
-
Patent number: 8815559Abstract: Compositions and methods relating to an alpha-amylase enzyme obtained from Nesterenkonia and related spp. are described.Type: GrantFiled: January 20, 2011Date of Patent: August 26, 2014Assignee: Danisco US Inc.Inventors: Brian E. Jones, Marc Kolkman
-
Publication number: 20140234906Abstract: The present invention relates to alpha-amylase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.Type: ApplicationFiled: October 17, 2012Publication date: August 21, 2014Inventors: Carsten Andersen, Signe Eskildsen Larsen, Esben Peter Friis, Peter Kamp Hansen, Henrik Friis Madsen, Anders Viksoe Nielsen, Pernille Ollendorf Micheelsen
-
Patent number: 8809031Abstract: A method of making a Bacillus alpha-amylase variant that increases alpha-amylase production and the alpha-amylases produced thereby. The recombinant alpha-amylases can be placed in compositions and used for purposes of laundry detergents, cleaning and dishwashing detergents, fabric desizing, starch liquefaction, cereal liquefaction, starch saccharification, biofilm removal, and starch hydrolysis in cane sugar processing.Type: GrantFiled: March 20, 2008Date of Patent: August 19, 2014Assignee: Danisco US Inc.Inventors: George England, Marc Kolkman, Casper Vroemen
-
Publication number: 20140220635Abstract: The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.Type: ApplicationFiled: October 17, 2012Publication date: August 7, 2014Applicant: Novozymes A/SInventors: Carsten Andersen, Signe Larsen, Esben Peter Friis, Pernille Ollendorff Micheelsen, Anders Viksoe Nielsen, Randall Deinhammer, Xinyu Shen
-
Publication number: 20140206026Abstract: The present invention relates to variants of a parent alpha-amylase. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.Type: ApplicationFiled: June 29, 2012Publication date: July 24, 2014Applicant: Novozymes A/SInventors: Svend Kaasgaard, Signe Eskildsen Larsen, Jens Oebro, Lars Beier, Connie Pontoppidan, Iben Damager, Carsten Andersen, Allan Svendsen
-
Patent number: 8785365Abstract: The present invention relates to ?-amylase variants that are stabilized to dimerization and/or multimerization, in particular at elevated temperatures or high pH, by point mutagenesis of positively polarized or charged or neutral surface amino acids to give more negatively polarized or charged amino acids. The invention further relates to methods of increasing the stability of an ?-amylase to dimerization and/or multimerization brought about by electrostatic interactions whereby at least one amino acid residue on the surface of the starting molecule, which makes a neutral or positively polar or charged contribution to the electrostatic potential of said molecule, is replaced with a more negatively polar or negatively charged amino acid residue. The ?-amylase variants obtained thereby exhibit better stability to influences of the solvent, increased processivity, and are suited for numerous industrial areas of use, in particular as active ingredients in detergents and cleansers.Type: GrantFiled: March 28, 2007Date of Patent: July 22, 2014Assignee: BASF SEInventors: Cornelius Bessler, Susanne Wieland, Karl-Heinz Maurer
-
Publication number: 20140200172Abstract: The invention relates to a novel Termamyl-like alpha-amylase, and Termamyl-like alpha-amylases comprising mutations in two, three, four, five or six regions/positions. The variants have increased thermostability at acidic pH and/or at low Ca2+ concentrations (relative to the parent).Type: ApplicationFiled: November 13, 2013Publication date: July 17, 2014Applicant: NOVOZYMES A/SInventors: Allan Svendsen, Torben Vedel Borchert, Henrik Bisgaard-Frantzen, Helle Outtrup, Bjarne Ronfeldt Nielsen, Vibeke Skovgaard Nielsen, Lisbeth Hedegaard
-
Publication number: 20140199752Abstract: A method of producing an enzyme of interest in a fed-batch cultivation comprising: a) cultivating a microorganism in a culture medium conducive to its growth wherein the microorganism produces the enzyme of interest; and b) adding a manganese compound to the culture medium one or more times during the cultivation.Type: ApplicationFiled: August 3, 2012Publication date: July 17, 2014Applicant: NOVOZYMES A/SInventors: Jon Martin Persson, Niels Banke
-
Publication number: 20140199751Abstract: A method for releasing the content of the periplasmic space of bacterial cells is provided, which comprises incubating the bacterial cells in a solution containing styrene maleic acid copolymer (SMA). Also provided is a method of preparing a substantially pure sample of recombinant polypeptide. The methods find application in the recovery of materials, such as proteins, from bacterial cells.Type: ApplicationFiled: May 9, 2012Publication date: July 17, 2014Inventors: Timothy Dafforn, Owen Robert Tyrynis-Thomas
-
Patent number: 8772001Abstract: Processes for production of an alcohol product from granular starch comprising a pre-treatment at an elevated temperature below the initial gelatinization temperature of the granular starch followed by simultaneous saccharification and fermentation, and optionally recovery of ethanol, are described.Type: GrantFiled: June 13, 2008Date of Patent: July 8, 2014Assignee: Novozymes A/SInventors: Hans Sejr Olsen, Sven Pedersen, Rikke Monica Festersen
-
Patent number: 8765439Abstract: A maltogenic ?-amylase from Trichoderma reesei (TrAA) and variants thereof in the presence of a glucoamylase are useful in the production of high-glucose syrups from liquefied starch, where the high-glucose syrups produced thereby contain at least about 97% glucose. In this process, TrAA advantageously suppresses the reversion of glucose to malto-oligosaccharides. Expression hosts and encoding nucleic acids useful for producing TrAA and its variants also are provided.Type: GrantFiled: October 19, 2012Date of Patent: July 1, 2014Assignee: Danisco US Inc.Inventors: Gang Duan, Kathy Qian, Martijn Scheffers, Jayarama K. Shetty, Pieter Van Solingen
-
Patent number: 8753859Abstract: This invention relates to polypeptides, more specifically amylase polypeptides and nucleic acids encoding these, and their uses e.g. as non-maltogenic exoamylases in producing food or feed products.Type: GrantFiled: November 2, 2011Date of Patent: June 17, 2014Assignee: Dupont Nutrition Biosciences APSInventors: Karsten Matthias Kragh, Anja Hemmingsen Kellet-Smith, René Mikkelsen, Rie Mejldal, Rikke L. Bundgaard Jenner
-
Publication number: 20140141489Abstract: The present invention relates to variants of a parent alpha-amylase. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.Type: ApplicationFiled: June 29, 2012Publication date: May 22, 2014Inventors: Svend Kaasgaard, Jens Oebro, Signe Eskildsen Larsen, Allan Svendsen, Annette Helle Johansen, Michael Skjoet, Carsten Andersen, Lars Beier, Esben Peter Friis, Miguel Duarte Guilherme Pereira Toscano, Mads Bjoernvad, Frank Winther Rasmussen, Liv Spaanger Christiansen
-
Publication number: 20140141490Abstract: Protein-polymer composite materials are provided according to embodiments of the present invention that include an admixture of a polymer resin, a surfactant and a non-aqueous organic solvent. An aqueous solution containing bioactive proteins is mixed with the admixture. The emulsion is mixed with a crosslinker to produce a curable composition. The curable composition is cured, thereby producing the protein-polymer composite material that is useful for facilitating removal of bioorganic stains.Type: ApplicationFiled: January 28, 2014Publication date: May 22, 2014Applicants: Toyota Motor Engineering & Manufacturing North America, Inc., Regents of the University of Minnesota, Toyota Motor CorporationInventors: Ping Wang, Songtao Wu, Hongfei Jia, Masahiko Ishii, Minjuan Zhang, Xiaodong Tong
-
Patent number: 8722381Abstract: Described are variants of alpha (?)-amylases having altered starch hydrolysis profiles. The variants have improved thermostability and increased specific activity, resulting in reduced peak viscosity and altered final viscosity during starch liquefaction. The amylase variants are useful, e.g., in liquefaction and other starch degradation processes.Type: GrantFiled: February 4, 2009Date of Patent: May 13, 2014Assignee: Danisco US Inc.Inventors: Sandra W. Ramer, Michael J. Pepsin, Andrew Shaw
-
Patent number: 8716208Abstract: A solid particulate laundry detergent composition including: (a) polyethylene glycol polymer including a polyethylene glycol backbone and polyvinyl acetate side chains, wherein the average molecular weight of the polyethylene glycol backbone is in the range of from 4,000 Da to 8,000 Da, wherein the molecular weight ratio of the polyethylene glycol backbone to the polyvinyl acetate side chains is in the range of from 1:1.2 to 1:2, and wherein the average number of graft sites per ethylene oxide units is in the range of from 0.2 to 0.4; (b) amylase with greater than 90% identity to the AA560 alpha amylase endogenous to Bacillus sp. DSM 12649 and including: (i) mutations at one or more of positions 9, 149, 182, 186, 202, 257, 295, 299, 323, 339 and 345; and (ii) mutations at four or more of positions 118, 183, 184, 195, 320 and 458; and (c) laundry detergent ingredients.Type: GrantFiled: March 1, 2011Date of Patent: May 6, 2014Assignee: The Procter & Gamble CompanyInventors: Michelle Meek, Philip Frank Souter, Neil Joseph Lant, Alan Thomas Brooker, Nigel Patrick Somerville Roberts
-
Publication number: 20140087429Abstract: The present disclosure relates to an enzyme blend comprising a low pH, thermostable alpha-amylase and a Bacillus licheniformis alpha-amylase. The blend can include at least about 1.0 Liquefon Unit (LU) of the B. licheniformis alpha-amylase for every 5.0 Modified Wohlgemuth Unit (MWU) of the low pH, thermostable alpha-amylase. The enzyme blend described is suitable for starch liquefaction and saccharification, ethanol production, and/or sweetener production, among other things. Also provided herein is a method of processing a starch by liquefying the starch with the low pH, thermostable alpha-amylase and the Bacillus licheniformis alpha-amylase, simultaneously or sequentially.Type: ApplicationFiled: August 29, 2013Publication date: March 27, 2014Applicant: Danisco US Inc.Inventors: Glenn E. Nedwin, Vivek Sharma, Jayarama K. Shetty
-
Patent number: 8680034Abstract: An automatic dishwashing detergent composition comprising: a. at least 0.1 mg of active protease per gram of composition, wherein the protease is a variant of a protease that has at least 70% identity with the amino acid sequence of SEQ ID NO:1. wherein said variant comprise variations in one or more of the following positions: 32, 33, 48-54, 58-62, 94-107, 116, 123-133, 150, 152-156, 158-161, 164, 169, 175-186, 197, 198, 203-216 as compared with the protease in SEQ ID NO:1; and b. at least 0.05 mg of an active low temperature amylase per gram of composition.Type: GrantFiled: July 25, 2011Date of Patent: March 25, 2014Assignee: The Procter & Gamble CompanyInventor: Philip Frank Souter
-
Publication number: 20140080194Abstract: The present invention relates to variants (mutants) of parent Termamyl-like alpha-amylases, which variant has alpha-amylase activity and exhibits altered properties relative to the parent alpha-amylase.Type: ApplicationFiled: November 21, 2013Publication date: March 20, 2014Applicant: NOVOZYMES A/SInventors: Allan Svendsen, Carsten Andersen, Thomas Thisted, Claus von der Osten
-
Publication number: 20140075601Abstract: The present invention relates to alpha-amylases, nucleic acids encoding the alpha-amylases, methods of producing the alpha-amylases, and methods of using the alpha-amylases.Type: ApplicationFiled: October 28, 2013Publication date: March 13, 2014Applicant: NOVOZYMES A/SInventors: Carsten Andersen, Thomas Agersten Poulsen
-
Publication number: 20140073553Abstract: The present invention relates to variants (mutants) of polypeptides, in particular Termamyl-like alpha-amylases, which variant has alpha-amylase activity and exhibits an alteration in at least one of the following properties relative to said parent alpha-amylase: substrate specificity, substrate binding, substrate cleavage pattern, thermal stability, pH/activity profile, pH/stability profile, stability towards oxidation, Ca2+ dependency, specific activity, and solubility, in particular under production conditions.Type: ApplicationFiled: November 12, 2013Publication date: March 13, 2014Applicant: Novozymes A/SInventors: Carsten Andersen, Torben Vedel Borchert, Bjarne Ronfeldt Nielsen
-
Patent number: 8663966Abstract: We disclose a food additive comprising a PS4 variant polypeptide, in which the PS4 variant polypeptide is derivable from a parent polypeptide having non-maltogenic exoamylase activity, in which the PS4 variant polypeptide comprises substitutions at the following positions: G121D, 134, 141, 157, 223, 307 and 334 with reference to the position numbering of a Pseudomonas saccharophilia exoamylase sequence shown as SEQ ID NO: 1.Type: GrantFiled: September 19, 2007Date of Patent: March 4, 2014Assignee: Dupont Nutrition Biosciences APSInventors: Karsten Matthias Kragh, Bo Spange Sørensen
-
Publication number: 20140057324Abstract: Variants of Bacillus sp. no. 707 alpha-amylase are provided that are produced more efficiently and thus more economically. Higher fermentation yields are achieved through introducing amino acid variations that promote solubility of the variant in a fermentation broth. Increased solubility allows more enzyme to remain in solution after expression in a host cell. This in turn increases the efficiency with which the expressed variant enzyme can be recovered from the fermentation broth.Type: ApplicationFiled: August 23, 2013Publication date: February 27, 2014Applicant: Danisco US Inc.Inventors: Wolfgang AEHLE, Neelam S. AMIN
-
Publication number: 20140030767Abstract: The present invention relates to variants (mutants) of parent Termamyl-like alpha-amylases, which variant has alpha-amylase activity and exhibits altered stability, in particular at high temperatures and/or at low pH relative, and/or low Ca2+ to the parent alpha-amylase.Type: ApplicationFiled: May 31, 2013Publication date: January 30, 2014Applicant: Novozymes A/SInventor: Novozymes A/S
-
Publication number: 20140024083Abstract: The invention relates to a variant of a parent Termamyl-like alpha-amylase, which variant has alpha-amylase activity and exhibits an alteration in at least one of the following properties relative to the parent alpha-amylase: substrate specificity, substrate binding, substrate cleavage pattern, thermal stability, pH/activity profile, pH/stability profile, stability towards oxidation, Ca2+ dependency and specific activity.Type: ApplicationFiled: July 29, 2013Publication date: January 23, 2014Inventors: Allan Svendsen, Torben Vedel Borchert, Henrik Bisgaard-Frantzen
-
Publication number: 20140017749Abstract: The present invention relates to processes for producing fermentation products from starch-containing material, wherein a thermostable alpha-amylase and optionally a thermostable protease are present and/or added during liquefaction. The invention also relates to a composition suitable for use in a process of the invention.Type: ApplicationFiled: December 21, 2011Publication date: January 16, 2014Inventors: Randy Deinhammer, Suzanne Clark, Mauricio Quiros, John Matthews, Anne Glud Hjulmand, Chee Leong Soong, Tomoko Matsui, Shinobu Takagi
-
Publication number: 20140017715Abstract: The invention relates to a variant of a parent Termamyl-like ?-amylase, which exhibits an alteration in at least one of the following properties relative to said parent ?-amylase: i) improved pH stability at a pH from 8 to 10.5; and/or ii) improved Ca2+ stability at pH 8 to 10.5, and/or iii) increased specific activity at temperatures from 10 to 60° C.Type: ApplicationFiled: March 5, 2013Publication date: January 16, 2014Applicant: NOVOZYMES A/SInventors: Torben Vedel Borchert, Allan Svendsen, Carsten Andersen, Bjarne Nielsen, Torben Lauesgaard Nissen, Soren Kjaerulff
-
Patent number: 8617837Abstract: The present invention relates to variants (mutants) of parent Termamyl-like alpha-amylases, which variant has alpha-amylase activity and exhibits altered properties relative to the parent alpha-amylase.Type: GrantFiled: April 25, 2012Date of Patent: December 31, 2013Assignee: Novozymes A/SInventors: Allan Svendsen, Carsten Andersen, Thomas Thisted, Claus Von Der Osten
-
Publication number: 20130344542Abstract: Described are variants (mutants) of a parent alpha-amylase having alpha-amylase activity and exhibiting altered properties relative to the parent alpha-amylase, and methods of use, thereof.Type: ApplicationFiled: May 21, 2013Publication date: December 26, 2013Inventors: Luis G. Cascao-Pereira, Claudine Chang, Clement Choy, James T. Kellis, JR., Brian E. Jones, Melodie Estabrook, Marc Kolkman, Chris Leeflang, Casper Vroemen, Walter Weyler
-
Publication number: 20130344544Abstract: In one aspect, the invention is directed to polypeptides having an amylase activity, polynucleotides encoding the polypeptides, and methods for making and using these polynucleotides and polypeptides. The polypeptides of the invention can be used as amylases to catalyze the hydrolysis of starch into sugars.Type: ApplicationFiled: August 28, 2013Publication date: December 26, 2013Applicant: Verenium CorporationInventors: Kevin A. GRAY, Nahla M. ABOUSHADI, James B. GARRETT
-
Publication number: 20130337112Abstract: The inventors have modified the amino acid sequence of a maltogenic alpha-amylase to obtain variants with improved properties, based on the three-dimensional structure of the maltogenic alpha-amylase Novamyl. The variants have altered physicochemical properties., e.g. an altered pH optimum, improved thermostability, increased specific activity, an altered cleavage pattern or an increased ability to reduce retrogradation of starch or staling of bread.Type: ApplicationFiled: August 27, 2013Publication date: December 19, 2013Applicant: Novozymes A/SInventors: Joel Cherry, Allan Svendsen, Carsten Andersen, Lars Beier, Torben Peter Frandsen
-
Publication number: 20130316406Abstract: Isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius are provided. Further provided are methods of at least partially degrading, cleaving, or removing polysaccharides, lignocellulose, cellulose, hemicellulose, lignin, starch, chitin, polyhydroxybutyrate, heteroxylans, glycosides, xylan-, glucan-, galactan-, or mannan-decorating groups using isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius.Type: ApplicationFiled: June 28, 2013Publication date: November 28, 2013Applicant: Battelle Energy Alliance, LLCInventors: David N. Thompson, William A. Apel, Vicki S. Thompson, David W. Reed, Jeffrey A. Lacey, Emily D. Henriksen
-
Patent number: 8580721Abstract: A solid particulate laundry detergent composition including: (a) substituted cellulosic polymer including carboxymethyl substituent groups, and having a degree of substitution (DS) of at least 0.55, and having a degree of blockiness (DB) of at least 0.35, and having a DS+DB is in the range of from 1.05 to 2.00; (b) amylase with greater than 90% identity to the AA560 alpha amylase endogenous to Bacillus sp. DSM 12649 and including: (i) mutations at one or more of positions 9, 149, 182, 186, 202, 257, 295, 299, 323, 339 and 345; and (ii) mutations at four or more of positions 118, 183, 184, 195, 320 and 458; and (c) laundry detergent ingredients.Type: GrantFiled: March 1, 2011Date of Patent: November 12, 2013Assignee: The Procter & Gamble CompanyInventors: Michelle Meek, Philip Frank Souter, Neil Joseph Lant, Alan Thomas Brooker, Nigel Patrick Somerville Roberts
-
Publication number: 20130280786Abstract: The present invention relates to novel enzymes and the uses thereof. The invention also relates to methods of producing such enzymes, coding nucleic acid molecules, recombinant cells and methods of transforming biomass from such materials. The invention is particularly suited to degrade biomass and/or to improve biomass degradation, and to produce bioenergy products or recombinant proteins. This invention also relates to various applications of the enzymes in the field of paper industry, textile industry as well as in the chemical and medical fields.Type: ApplicationFiled: November 8, 2011Publication date: October 24, 2013Applicant: DEINOVEInventors: Jean-Paul Leonetti, Jean-Michel Claverie, Pascale Joseph, Lucie Roux
-
Publication number: 20130267002Abstract: The present invention relates to polypeptides having glucoamylase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: June 12, 2013Publication date: October 10, 2013Inventors: Sara Landvik, Marc Dominique Morant, Keiichi Ayabe, Guillermo Coward-Kelly
-
Patent number: 8551754Abstract: In one aspect, the invention is directed to polypeptides having an amylase activity, polynucleotides encoding the polypeptides, and methods for making and using these polynucleotides and polypeptides. The polypeptides of the invention can be used as amylases to catalyze the hydrolysis of starch into sugars.Type: GrantFiled: October 27, 2011Date of Patent: October 8, 2013Assignee: Verenium CorporationInventors: Kevin Gray, Nahla Aboushadi, James Garrett
-
Patent number: 8546099Abstract: The inventors have modified the amino acid sequence of a maltogenic alpha-amylase to obtain variants with improved properties, based on the three-dimensional structure of the maltogenic alpha-amylase Novamyl. The variants have altered physicochemical properties, e.g. an altered pH optimum, improved thermostability, increased specific activity, an altered cleavage pattern or an increased ability to reduce retrogradation of starch or staling of bread.Type: GrantFiled: June 26, 2012Date of Patent: October 1, 2013Assignee: Novozymes A/SInventors: Joel Cherry, Allan Svendsen, Carsten Andersen, Lars Beier, Torben Peter Frandsen
-
Patent number: 8545907Abstract: The present disclosure relates to an enzyme blend comprising a low pH, thermostable alpha-amylase and a Bacillus licheniformis alpha-amylase. The blend can include at least about 1.0 Liquefon Unit (LU) of the B. licheniformis alpha-amylase for every 5.0 Modified Wohlgemuth Unit (MWU) of the low pH, thermostable alpha-amylase. The enzyme blend described is suitable for starch liquefaction and saccharification, ethanol production, and/or sweetener production, among other things. Also provided herein is a method of processing a starch by liquefying the starch with the low pH, thermostable alpha-amylase and the Bacillus licheniformis alpha-amylase, simultaneously or sequentially.Type: GrantFiled: July 27, 2010Date of Patent: October 1, 2013Assignee: Danisco US Inc.Inventors: Glenn E. Nedwin, Vivek Sharma, Jayarama K. Shetty
-
Publication number: 20130252850Abstract: The invention relates to a variant of a parent Termamyl-like alpha-amylase, comprising mutations in two, three, four, five or six regions/positions. The variants have increased stability at high temperatures (relative to the parent). The invention also relates to a DNA construct comprising a DNA sequence encoding an alpha-amylase variant of the invention, a recombinant expression vector which carries a DNA construct of the invention, a cell which is transformed with a DNA construct of the invention, the use of an alpha-amylase variant of the invention for washing and/or dishwashing, textile desizing, starch liquefaction, a detergent additive comprising an alpha-amylase variant of the invention, a manual or automatic dishwashing detergent composition comprising an alpha-amylase variant of the invention, a method for generating a variant of a parent Termamyl-like alpha-amylase, which variant exhibits increased.Type: ApplicationFiled: May 20, 2013Publication date: September 26, 2013Applicant: NOVOZYMES A/SInventors: Allan Svendsen, Soren Kjaerulff, Henrik Bisgaard-Frantzen, Carsten Andersen
-
Patent number: 8513175Abstract: The invention relates to a variant of a parent Termamyl-like alpha-amylase, which variant has alpha-amylase activity and exhibits an alteration in at least one of the following properties relative to the parent alpha-amylase: substrate specificity, substrate binding, substrate cleavage pattern, thermal stability, pH/activity profile, pH/stability profile, stability towards oxidation, Ca2+ dependency and specific activity.Type: GrantFiled: July 25, 2012Date of Patent: August 20, 2013Assignee: Novozymes A/SInventors: Allan Svendsen, Torben Vedel Borchert, Henrik Bisgard-Frantzen
-
Patent number: 8507244Abstract: Variants of Bacillus sp. TS-23 strain alpha-amylases exhibit improved enzymatic performance, including increased thermostability, reduced calcium dependence, increased washing/cleaning performance, and baking ability. Compositions comprising these variants are useful in methods of starch processing, starch liquefaction, fermatation, starch saccharification, cleaning, laundrying, textile desizing, baking, and biofilm removal.Type: GrantFiled: March 2, 2012Date of Patent: August 13, 2013Assignee: Danisco US Inc.Inventors: Andrew Shaw, Sandra Ramer, Scott D. Power, Claudine Chang, Melodie Estabrook, Brian E. Jones, Clement Choy, Marc Kolkmam, Chris Leeflang, Casper Vroeman, Walter Weyler, Karsten Kraugh, Mansi Goyal, Thomas Graycar, Victoria Huang