Acting On Alpha-1, 4-glucosidic Bond, (e.g., Hyaluronidase, Invertase, Amylase, Etc. (some 3.2.1)) Patents (Class 435/201)
  • Patent number: 11407986
    Abstract: The present invention relates to polypeptide having alpha-amylase activity. The present invention also relates to polynucleotides encoding the polypeptides; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the polypeptides.
    Type: Grant
    Filed: December 29, 2016
    Date of Patent: August 9, 2022
    Assignee: Novozymes A/S
    Inventors: Torsten Bak Regueira, Bitten Plesner, Thomas Holberg Blicher, Anne Dorte Houg, Sofia Arnehed, Lars Lehmann Hylling Christensen, Carsten Andersen
  • Patent number: 11072786
    Abstract: The present invention relates to alpha-amylase variants with improved stability in the presence of glucose and/or relatively higher activity on long chain versus short chain substrates. The present encoding invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
    Type: Grant
    Filed: April 10, 2015
    Date of Patent: July 27, 2021
    Assignee: Novozymes A/S
    Inventors: Carsten Andersen, Paria Saunders
  • Patent number: 11040114
    Abstract: The present invention provides new forms of human ?-N-acetylgalactosaminidase (NAGAL) polypeptide or a functionally active variant or fragment thereof, nucleic acids encoding the same, and related products and uses, including use in methods of treating Fabry disease, Schindler disease or Kanzaki disease.
    Type: Grant
    Filed: December 22, 2016
    Date of Patent: June 22, 2021
    Inventors: Wouter Vervecken, Steven Geysens
  • Patent number: 10918124
    Abstract: Improved method for purifying extracts containing phytoglycogen or certain derivatives thereof which comprises the step of subjecting the extracts to ultrafiltration. Methods for providing a lower viscosity phytoglycogen material by subjecting an extract containing phytoglycogen (or purified phytoglycogen) to beta-amylase treatment. Methods for preparing beta-dextrins or derivatives thereof from phytoglycogens or derivatives thereof.
    Type: Grant
    Filed: August 2, 2012
    Date of Patent: February 16, 2021
    Assignee: PURDUE RESEARCH FOUNDATION
    Inventor: Yuan Yao
  • Patent number: 10681914
    Abstract: The present specification discloses plant agent compositions, articles of manufacture, containers or kits comprising such compositions, and methods and uses to control a causal agent of a plant disease or increasing plant growth and/or fruit production.
    Type: Grant
    Filed: August 22, 2016
    Date of Patent: June 16, 2020
    Assignee: Neozyme International, Inc.
    Inventors: Parker Dale, Parker David Dale
  • Patent number: 10557234
    Abstract: The present specification discloses papermaking additive compositions, articles of manufacture, containers or kits comprising such compositions, and methods and uses to increase separation of cellulose fibers from a pulp, to remove one or more impurities and/or one or more contaminates from a pulp and/or a paper material and to remove an ink from a pulp and/or a paper material.
    Type: Grant
    Filed: August 22, 2016
    Date of Patent: February 11, 2020
    Assignee: Neozyme International, Inc.
    Inventors: Parker Dale, Parker David Dale
  • Patent number: 10538751
    Abstract: The present invention relates to alpha-amylase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
    Type: Grant
    Filed: June 4, 2014
    Date of Patent: January 21, 2020
    Assignee: NOVOZYMES A/S
    Inventor: Carsten Andersen
  • Patent number: 10525167
    Abstract: The present invention discloses a low viscosity composition comprising enamel matrix derivative (EMD) proteins in a suitable pharmaceutical carrier, said composition having a pH between pH 3.8-4.2, and a viscosity of less than 50 mPa s at 22° C., which, when applied to a porous bone graft material, improves and/or enhances bioactivity and osteoconduction of said bone graft material. The present invention consequently discloses a bone graft material which has been bioactivated using a low viscosity composition comprising enamel matrix derivative (EMD) proteins and which displays enhanced biocompatibility and improved bone regeneration after implantation.
    Type: Grant
    Filed: July 9, 2015
    Date of Patent: January 7, 2020
    Assignee: STRAUMANN HOLDING AG
    Inventors: Alessandra Apicella, Peggy Heunemann
  • Patent number: 10463722
    Abstract: The present disclosure provides compositions comprising mutant fungi include an inactive form of the sterylglucosidase enzyme. The present disclosure is also directed to vaccine based compositions, which include a mutant fungus that prohibit pathogenic fungal infection. This disclosure also provides methods for administering these compositions as a prophylaxis against fungal infection, as well as methods for isolating sterylglucosides that include the use of such mutant fungal compositions.
    Type: Grant
    Filed: August 10, 2017
    Date of Patent: November 5, 2019
    Assignee: The Research Foundation for The State University of New York
    Inventors: Maurizio Del Poeta, Antonella Rella
  • Patent number: 10316065
    Abstract: The invention is related to an oncolytic adenovirus that comprises a sequence encoding a hyaluronidase enzyme inserted in its genome. This adenovirus spreads more efficiently in the tumour mass and therefore the oncolytic effect is increased. Injecting the oncolytic adenovirus of the invention endovenously results in tumour volume regressions. Therefore, the oncolytic adenovirus of the present invention is useful for the treatment of a cancer or a pre-malignant state of cancer.
    Type: Grant
    Filed: May 5, 2010
    Date of Patent: June 11, 2019
    Assignees: Fundacio Institut d Investigacio Biomedica de Bellvitge (IDIBELL), Institut Catala D Oncologia
    Inventors: Sònia Guedan Carrió, Manel Maria Cascallo Piqueras, Ramon Alemany Bonastre
  • Patent number: 10265410
    Abstract: Provided herein are methods for ameliorating an adverse effect of systemic administration of a PEG hyaluronan degrading enzyme to a subject. The methods involve systemically administering a PEGylated hyaluronan degrading enzyme, particularly a PEGylated hyaluronidase, such as any of the animal or bacterial hyaluronidases, to the subject and administering an amount of a corticosteroid sufficient to ameliorate the adverse effect. Also provided are method of treating a hyaluronan-associated disease or condition for single-agent therapy or combination therapy.
    Type: Grant
    Filed: April 15, 2016
    Date of Patent: April 23, 2019
    Assignee: Halozyme, Inc.
    Inventors: Harold Michael Shepard, Curtis Thompson, Xiaoming Li, Gregory I. Frost
  • Patent number: 10208300
    Abstract: A process for preparing Streptomyces koganeiensis ATCC 31394 hyaluronidase by obtaining hyaluronidase having molecular weight of 21.6 kDalton, which has hyaluronidase activity and stability markedly higher than those of the hyaluronidase obtained from such microorganism to date.
    Type: Grant
    Filed: August 2, 2016
    Date of Patent: February 19, 2019
    Assignee: FIDIA FARMACEUTICI S.P.A.
    Inventors: Luciano Messina, Susanna Vaccaro, Salvatore Caruso, Giovanni Gennari
  • Patent number: 10137104
    Abstract: Provided herein is combination cancer therapy effected by administering a polymer-conjugated hyaluronidase, and a tumor-targeted taxane, and optionally a further chemotherapeutic agent such as a nucleoside analog. The combination therapy can be used in methods of treating cancers, and in particular solid tumor cancers.
    Type: Grant
    Filed: June 16, 2017
    Date of Patent: November 27, 2018
    Assignee: Halozyme, Inc.
    Inventors: Daniel C. Maneval, H. Michael Shepard, Curtis B. Thompson
  • Patent number: 10100293
    Abstract: In one aspect, the invention is directed to polypeptides having an amylase activity, polynucleotides encoding the polypeptides, and methods for making and using these polynucleotides and polypeptides. The polypeptides of the invention can be used as amylases to catalyze the hydrolysis of starch into sugars.
    Type: Grant
    Filed: December 16, 2015
    Date of Patent: October 16, 2018
    Assignee: BASF Enzymes LLC
    Inventors: Kevin A. Gray, Nahla M. Aboushadi, James B. Garrett
  • Patent number: 10066237
    Abstract: The present invention relates to isolated polypeptides having beta-xylosidase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Grant
    Filed: June 13, 2017
    Date of Patent: September 4, 2018
    Assignee: Novozymes Inc.
    Inventors: Yu Zhang, Ye Liu, Junxin Duan, Lan Tang, Brett McBrayer
  • Patent number: 9969998
    Abstract: Provided are variant adenosine deaminase 2 (ADA2) proteins, conjugates thereof and compositions containing the proteins and/or conjugates. Also provided are methods and uses of the ADA2 proteins or conjugates for treating diseases and conditions, such as a tumor or cancer, and in particular any disease or condition associated with elevated adenosine or other associated marker.
    Type: Grant
    Filed: April 8, 2016
    Date of Patent: May 15, 2018
    Assignee: Halozyme, Inc.
    Inventors: Christopher D. Thanos, Lin Wang, H. Michael Shepard
  • Patent number: 9913822
    Abstract: Provided herein is combination therapy containing an anti-hyaluronan agent, such as a polymer-conjugated hyaluronan-degrading enzyme, and a tumor-targeted taxane, and optionally a further chemotherapeutic agent such as a nucleoside analog. The combination therapy can be used in methods of treating cancers, and in particular solid tumor cancers.
    Type: Grant
    Filed: March 15, 2013
    Date of Patent: March 13, 2018
    Assignee: Halozyme, Inc.
    Inventors: Daniel C. Maneval, H. Michael Shepard, Curtis B. Thompson
  • Patent number: 9878046
    Abstract: Provided herein are methods for ameliorating an adverse effect of systemic administration of a PEG hyaluronan degrading enzyme to a subject. The methods involve systemically administering a PEGylated hyaluronan degrading enzyme, particularly a PEGylated hyaluronidase, such as any of the animal or bacterial hyaluronidases, to the subject and administering an amount of a corticosteroid sufficient to ameliorate the adverse effect. Also provided are method of treating a hyaluronan-associated disease or condition for single-agent therapy or combination therapy.
    Type: Grant
    Filed: July 15, 2011
    Date of Patent: January 30, 2018
    Assignee: Halozyme, Inc.
    Inventors: Harold Michael Shepard, Curtis Thompson, Xiaoming Li, Gregory I. Frost
  • Patent number: 9719103
    Abstract: Four genes, A622, NBB1, PMT, and QPT, can be influenced for increasing nicotinic alkaloid levels in Nicotiana plants, as well as for synthesizing nicotinic alkaloids in non-nicotine producing plants and cells. In particular, overexpressing one or more of A622, NBB1, PMT, and QPT may be used to increase nicotine and nicotinic alkaloid levels in tobacco plants. Non-nicotine producing cells can be engineered to produce nicotine and related compounds by overexpressing A622 and NBB1.
    Type: Grant
    Filed: April 15, 2015
    Date of Patent: August 1, 2017
    Assignee: 22nd Century Limited, LLC
    Inventors: Takashi Hashimoto, Masataka Kajikawa
  • Patent number: 9688975
    Abstract: The present invention relates to beta-glucosidase variants, e.g. beta-glucosidase variants of a parent Family GH3A beta-glucosidase from Aspergillus fumigatus. The present invention also relates to polynucleotides encoding the beta-glucosidase variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the beta-glucosidase variants.
    Type: Grant
    Filed: September 30, 2011
    Date of Patent: June 27, 2017
    Assignee: Novozymes, Inc.
    Inventors: Mark Wogulis, Paul Harris, David Osborn
  • Patent number: 9540668
    Abstract: A reduced sugar syrup having an advantageously low viscosity is prepared by hydrolysis of starch or starchy material using a particular type of alpha amylase enzyme which yields a saccharide distribution having a low DP1-2 and low DP11+ content. The DP4 content of the syrup may be favorably increased by using a maltotetragenic alpha amylase enzyme in combination with the aforementioned alpha amylase enzyme. The syrup is useful in the production of food, beverage, animal feed, animal health and nutrition, pharmaceutical, and cosmetic compositions and may be combined with a high intensity sweetener to provide a composition capable of being substituted for conventional corn syrups.
    Type: Grant
    Filed: January 29, 2013
    Date of Patent: January 10, 2017
    Assignees: Tate & Lyle Ingredients Americas LLC, Verenium Corporation
    Inventors: Rohit Medhekar, Andrew Joseph Hoffman
  • Patent number: 9458442
    Abstract: Methods and diagnostic agents for identification of subjects for cancer treatment with an anti-hyaluronan agent, such as a hyaluronan-degrading enzyme, are provided. Diagnostic agents for the detection and quantification of hyaluronan in a biological sample and monitoring cancer treatment with an anti-hyaluronan agent, for example a hyaluronan-degrading enzyme, are provided. Combinations and kits for use in practicing the methods also are provided.
    Type: Grant
    Filed: August 14, 2014
    Date of Patent: October 4, 2016
    Assignee: Halozyme, Inc.
    Inventors: Ping Jiang, H. Michael Shepard, Lei Huang
  • Patent number: 9422536
    Abstract: The invention relates to polypeptides having glucanase, e.g., endoglucanase, mannanase, xylanase activity or a combination of these activities, and polynucleotides encoding them. In one aspect, the glucanase activity is an endoglucanase activity (e.g., endo-1,4-beta-D-glucan 4-glucano hydrolase activity) and comprises hydrolysis of 1,4-beta-D-glycosidic linkages in cellulose, cellulose derivatives (e.g., carboxy methyl cellulose and hydroxy ethyl cellulose) lichenin, beta-1,4 bonds in mixed beta-1,3 glucans, such as cereal beta-D-glucans or xyloglucans and other plant material containing cellulosic parts. In addition, methods of designing new enzymes and methods of use thereof are also provided. In alternative aspects, the new glucanases e.g., endoglucanases, mannanases, xylanases have increased activity and stability at increased pH and temperature.
    Type: Grant
    Filed: September 5, 2013
    Date of Patent: August 23, 2016
    Assignees: BP Corporation North America Inc., Syngenta Participations AG
    Inventors: Brian Steer, Walter Niles Callen, Shaun Healey, Derrick Pulliam
  • Patent number: 9278124
    Abstract: Provided herein are diagnostic methods for identifying subjects susceptible to treatment with a hypoxia-activated agent, and related methods. Also provided herein are methods of monitoring treatments with anti-hyaluronan agents, and related methods.
    Type: Grant
    Filed: October 16, 2013
    Date of Patent: March 8, 2016
    Assignee: Halozyme, Inc.
    Inventors: H. Michael Shepard, Xiaoming Li, Curtis Thompson
  • Patent number: 9279111
    Abstract: The present invention provides a novel leech HAase and a method of producing low-molecular-weight HA oligosaccharides using the leech HAase. This invention successfully cloned the first leech HAase gene and provides a method for high-level expression of the leech HAase gene. By controlling the incubation condition, different HA oligosaccharides, particularly HA4, HA6, HA8 and HA10, can be selectively generated using the leech HAase. The large-scale expression of the leech HAase and the enzymatic production of specific HA oligosaccharides are not only useful for the cosmetic, healthcare and the medical industries but also can be a great help to polysaccharides chemical synthesis and cancer research.
    Type: Grant
    Filed: April 12, 2014
    Date of Patent: March 8, 2016
    Assignee: Jiangnan University
    Inventors: Jian Chen, Guocheng Du, Zhen Kang, Peng Jin
  • Patent number: 9157074
    Abstract: To provide novel ?-amylase derived from a microorganism and a gene thereof. ?-amylase derived from Bacillus flexus is provided.
    Type: Grant
    Filed: June 17, 2013
    Date of Patent: October 13, 2015
    Assignee: Amano Enzyme Inc.
    Inventors: Akiko Matsunaga, Hitoshi Amano, Shotaro Yamaguchi
  • Patent number: 9096837
    Abstract: The present disclosure relates to variants (mutants) of polypeptides, in particular Termamyl-like alpha-amylases, which variant has alpha-amylase activity and exhibits an alteration in at least one of the following properties relative to said parent alpha-amylase: substrate specificity, substrate binding, substrate cleavage pattern, thermal stability, pH/activity profile, pH/stability profile, stability towards oxidation, Ca2+ dependency, specific activity, in particular laundry and dish-wash applications.
    Type: Grant
    Filed: January 18, 2011
    Date of Patent: August 4, 2015
    Assignee: Novozymes A/S
    Inventors: Carsten Andersen, Henrik Ostdal, Peter Skagerlind
  • Patent number: 9090886
    Abstract: To provide novel ?-amylase derived from a microorganism and a gene thereof. ?-amylase derived from Bacillus flexus is provided.
    Type: Grant
    Filed: June 17, 2013
    Date of Patent: July 28, 2015
    Assignee: Amano Enzyme Inc.
    Inventors: Akiko Matsunaga, Hitoshi Amano, Shotaro Yamaguchi
  • Patent number: 9062295
    Abstract: In one aspect, the invention is directed to polypeptides having an amylase activity, polynucleotides encoding the polypeptides, and methods for making and using these polynucleotides and polypeptides. In one aspect, the polypeptides of the invention can be used as amylases, for example, alpha amylases, to catalyze the hydrolysis of starch into sugars.
    Type: Grant
    Filed: November 6, 2012
    Date of Patent: June 23, 2015
    Assignee: BASF Enzymes LLC
    Inventors: Walter Callen, Toby Richardson, Gerhard Frey, Carl A. Miller, Martin Kazaoka, Eric J. Mathur, Jay M. Short
  • Publication number: 20150147780
    Abstract: Disclosed herein are embodiments for a novel method of producing an organic compound, including harvesting at least one organic compound from an organism or cell line genetically engineered with a gene for at least one proton-pump protein.
    Type: Application
    Filed: November 24, 2014
    Publication date: May 28, 2015
    Inventors: Eugene Dinescu, Vincent Dinescu
  • Publication number: 20150147783
    Abstract: The present invention relates to an isolated mutant eubacterium comprising at least one mutation resulting in a substitution of at least one amino acid in the beta-subunit of the RNA-polymerase encoded for by the rpoB-gene providing an altered production of a product of interest when said production of a product of interest is compared to the production of the same product in an isogenic wild type strain grown at identical conditions, wherein the substitution of at least one amino acid occurs at any of positions 469, 478, 482, 485, or 487 of SEQ ID NO:2, or at the equivalent positions in any eubacterial RNA-polymerase beta-subunit family member. Another aspect of the invention relates to a process for producing at least one product of interest in a mutant eubacterium and to a use of the mutant eubacterium according to the invention for producing at least one product of interest.
    Type: Application
    Filed: February 9, 2015
    Publication date: May 28, 2015
    Inventors: Steen Troels Joergensen, Jens Toenne Andersen, Niels Banke, Preben Nielsen
  • Publication number: 20150147308
    Abstract: Described herein are compositions which include digestive enzymes and which are formulated to reduce one or more symptoms of a neuropsychiatric disorder. Also described herein is a method for treating an individual with a neuropsychiatric disorder using digestive enzymes and their derivatives to alleviate the symptoms of neuropsychiatric disorders. The method comprises administering to the individual an effective amount of digestive enzymes that are either naturally or recombinantly derived, or their derivatives, in an amount effective to reduce one or more symptoms of the neuropsychiatric disorder.
    Type: Application
    Filed: February 3, 2015
    Publication date: May 28, 2015
    Inventors: Joan M. Fallon, Matthew Heil, James Szigethy, James Fallon
  • Publication number: 20150140572
    Abstract: The present invention relates to a composition having the activity of degrading the cell wall of a Mycobacterium species comprising: (a) a first fusion protein including (i) a domain with a first enzymatic activity, the enzymatic activity being at least one or more of the following: N-acetyl-b-D-muramidase (lysozyme, lytic transglycosylase), N-acetyl-b-D-glucosaminidase, N-acetylmuramoyl-L-alanine amidase, L-alanoyl-D-glutamate (LD) endopeptidase, c-D-glutamyl-meso-diaminopimelic acid (DL) peptidase, D-Ala-m-DAP (DD) endopeptidase, or m-DAP-m-DAP (LD) endopeptidase, (ii) at least one peptide stretch fused to the N- or C-terminus of the domain with the first enzymatic activity; and (iii) a protein transduction domain (PTD) being at the N- or C-terminus of the first fusion protein; and (b) a second fusion protein including (i) a domain with a second enzymatic activity, the enzymatic activity being at least one or more of the following: lipolytic activity, cutinase, mycolarabinogalactanesterase, or alpha/beta hy
    Type: Application
    Filed: July 1, 2013
    Publication date: May 21, 2015
    Inventors: Stefan Miller, Robert Andreas Fischer
  • Publication number: 20150140637
    Abstract: The present invention relates to a process for the separation of a mixture of a protein and its reaction product with a polyalkylene glycol, comprising the steps: a) providing a mixture comprising the protein and the reaction product in a fluid, b) bringing the mixture of step a) into contact with a microporous cellulose acetate membrane with adsorption of the reaction product onto the cellulose acetate membrane, the protein not becoming adsorbed onto the cellulose acetate membrane, c) removal of the protein from the cellulose acetate membrane, and d) desorbing the reaction product from the cellulose acetate membrane, the reaction product being selected from the group comprising the protein which is mono- or polyfunctionalized by the polyalkylene glycol, or mixtures thereof.
    Type: Application
    Filed: March 21, 2012
    Publication date: May 21, 2015
    Inventors: Wolfgang Demmer, Louis Villain, Hans-Heinrich Hoerl
  • Publication number: 20150139977
    Abstract: The invention relates to enzymes having xylanase, mannanase and/or glucanase activity, e.g., catalyzing hydrolysis of internal ?-1,4-xylosidic linkages or endo-?-1,4-glucanase linkages; and/or degrading a linear polysaccharide beta-1,4-xylan into xylose. Thus, the invention provides methods and processes for breaking down hemicellulose, which is a major component of the cell wall of plants, including methods and processes for hydrolyzing hemicelluloses in any plant or wood or wood product, wood waste, paper pulp, paper product or paper waste or byproduct. In addition, methods of designing new xylanases, mannanases and/or glucanases and methods of use thereof are also provided. The xylanases, mannanases and/or glucanases have increased activity and stability at increased pH and temperature.
    Type: Application
    Filed: October 22, 2014
    Publication date: May 21, 2015
    Inventors: David Weiner, David Blum, Alexander Varvak, Shaun Healey, Kristine Chang, Geoff Hazlewood, Thomas Todaro, Grace Desantis, Hwai Chang, Connie Jo Hansen, Scott W. Beaver, Thomas Woodward, Charles Hancock
  • Publication number: 20150141680
    Abstract: The present invention provides compositions and methods related to the production and use of enzymes suitable for reducing the amount of steryl glycosides or saturated monoacyl glycerols in a lipid mixture.
    Type: Application
    Filed: March 14, 2013
    Publication date: May 21, 2015
    Applicant: KECLON S.A.
    Inventors: Hugo Menzella, Salvador Peiru, Leandro Vetcher
  • Publication number: 20150133531
    Abstract: The present invention provides a method of expressing at least one heterologous nucleic acid sequence in a cell, the method comprising introducing at least one heterologous nucleic acid sequence into a cell by infecting said cell with a recombinant negative-strand RNA virus vector comprising said at least one heterologous nucleic acid sequence, wherein the recombinant negative-strand RNA virus vector includes a viral genome coding for a mutated P protein, which leads to a loss of the viral genome replication ability without a loss of the viral transcription ability, and wherein said at least one heterologous nucleic acid sequence encodes a cellular reprogramming or programming factor or a therapeutic protein. In addition, the present invention provides a cell or a population of cells prepared in vitro by said method as well as a pharmaceutical composition comprising said cell or population of cells.
    Type: Application
    Filed: May 24, 2013
    Publication date: May 14, 2015
    Applicant: AmVac AG
    Inventor: Marian Wiegand
  • Patent number: 9029115
    Abstract: The pharmaceutical use of lipases related to the Thermomyces lanuginosus (Humicola lanuginosa) lipase comprising amino acids 1-269 of SEQ ID NO: 2, optionally in combination with a protease and/or an amylase. Examples of medical indications are: Treatment of digestive disorders, pancreatic exocrine insufficiency (PEI), pancreatitis, cystic fibrosis, diabetes type I, and/or diabetes type II. The lipases of the invention have, e.g., an improved digestion performance in vitro, an improved activity at a pH in the neutral range, an improved stability at low pH, and are stable against protease-degradation, and/or are stable in the presence of pepsin and bile salts. The invention also relates to methods of determining digestion performance in vitro of lipases, as well as to certain novel variants of the lipase of T. lanuginosus.
    Type: Grant
    Filed: August 16, 2012
    Date of Patent: May 12, 2015
    Assignees: Novozymes A/S, Novozymes Inc
    Inventors: Allan Svendsen, Michael Skjoet, Deborah Yaver, Lars Lehmann Hylling Christensen, Signe Eskildsen Larsen, Nina Lundin, Michael Lamsa, Peter Colin Gregory
  • Publication number: 20150125902
    Abstract: Provided are isolated polypeptides having glucoamylase activity, catalytic domains, and polynucleotides encoding the polypeptides, catalytic domains. Also provided are nucleic acid constructs, vectors and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains.
    Type: Application
    Filed: August 24, 2012
    Publication date: May 7, 2015
    Applicant: Novozymes A/S
    Inventors: Tianqi Sun, Ming Li
  • Publication number: 20150125925
    Abstract: The present invention provides consumer products comprising a serine protease variant, more specifically subtilisin variants produced there from. Specifically, the present invention provides consumer products comprising a serine protease variant, more specifically a subtilisin variant having one or more substitutions as compared to a reference serine protease. In addition, the present invention provides methods of making such compositions and methods of treating surfaces, particularly fabric surfaces comprising contacting a surface with an aqueous liquor comprising a serine protease variant, more specifically subtilisin variants and an adjunct material.
    Type: Application
    Filed: November 5, 2013
    Publication date: May 7, 2015
    Applicant: The Procter & Gamble Company
    Inventors: Philip Frank SOUTER, Euan John MAGENNIS, Glenn Steven WARD, Neelam S. AMIN, Katherine AUGUSTYN, Joshua R. BASLER, Luis Gustavo CASCAO-PEREIRA, Katherine D. COLLIER, Edward M. CONCAR, David A. ESTELL, James T. KELLIS, JR., Alexander PISARCHIK, Ayrookaran Joseph POULOSE, Jian YAO
  • Publication number: 20150118730
    Abstract: The present invention is directed to enzyme based methods for separating protein from protein-rich materials derived from plant seeds, fruit, or other biomass and products made therefrom. The protein content in the resulting products is improved by separating and removing the carbohydrates from around the proteins in, for example, soybean meal. This removal is facilitated by the enzymatic hydrolysis of poly- and oligomeric carbohydrates into monosaccharides and other water soluble sugars. The present invention provides for the production of three streams of useful materials. The first is an enriched protein material comparable to the known SPCs but without significant quantities of undigestible oligosaccharides and polysaccharides. The second is an SPI made from the soluble protein in the hydrolysate which is valuable for high-quality feed, food and industrial uses.
    Type: Application
    Filed: May 9, 2013
    Publication date: April 30, 2015
    Applicant: THE UNIVERSITY OF AKRON
    Inventors: Lu-Kwang Ju, Abdullah Loman, Anthony Coffman, Qian Li, Srujana Koganti
  • Publication number: 20150111259
    Abstract: The present teachings provide direct conversion of granular starch into a soluble sugar composition comprising a high, very high, and/or ultra high maltose content. The method involves contacting an aqueous slurry of granular starch with an enzyme composition comprising an appropriate ratio of an alpha-amylase and a maltogenic enzyme to produce a soluble starch substrate that is enzymatically converted to a sugar composition containing higher maltose. The process may use more than one temperature to achieve the same.
    Type: Application
    Filed: March 12, 2013
    Publication date: April 23, 2015
    Applicant: DANISCO US INC.
    Inventors: T. Kleinhout, Bart Christiaan Koops, Floor K. Kooy, Sung Ho Lee, Jayarama K. Shetty, Bruce A. Strohm
  • Publication number: 20150110765
    Abstract: The present invention relates to isolated polypeptides having alpha-glucuronidase activity, catalytic domains and polynucleotides encoding the polypeptides, catalytic domains. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides or catalytic domains.
    Type: Application
    Filed: April 22, 2013
    Publication date: April 23, 2015
    Inventors: Johan Börjesson, Anders Vikø-Nielsen, Nikolaj Spodsberg, Kristian Krogh
  • Patent number: 9011948
    Abstract: Process for producing a dairy product by adding a lactase to a dairy product which contains lactose, the lactase added having less than 40 units arylsulfatase activity per NLU of lactase activity.
    Type: Grant
    Filed: November 28, 2006
    Date of Patent: April 21, 2015
    Assignee: DSM IP Assets B.V.
    Inventors: Petrus Jacobus Theodorus Dekker, Luppo Edens, Maximiliaan Peter Marie De Swaaf, Albertus Alard Van Dijk
  • Patent number: 9012197
    Abstract: The present invention is directed to recombinant microalgal cells and their use in production of heterologous hemagglutinin-neuraminidase (HN) polypeptides, as well as compositions and uses thereof.
    Type: Grant
    Filed: December 28, 2010
    Date of Patent: April 21, 2015
    Assignees: Merial, Inc., Sanofi Vaccine Technologies, S.A.S.
    Inventors: Anne-Cécile V. Bayne, James Casey Lippmeier, Kirk Emil Apt, Xuan Guo, Joyce A. Pritchard
  • Publication number: 20150104833
    Abstract: By combination of hydrophobic chromatography and strongly basic anion-exchange chromatography, a novel, highly hydrophobic ?-glucosidase was successfully identified from Acremonium cellulolyticus. Further, a gene corresponding to the identified ?-glucosidase was isolated. When multiple modifications were introduced into the base sequence of the gene, the gene was successfully expressed in Trichoderma viride at a high level, and the expression product successfully exhibited a high ?-glucosidase activity.
    Type: Application
    Filed: December 19, 2014
    Publication date: April 16, 2015
    Applicant: MEIJI SEIKA PHARMA CO., LTD.
    Inventors: Fumikazu YOKOYAMA, Kengo YOKOYAMA, Nobuko MAZUKA
  • Publication number: 20150093790
    Abstract: The disclosure provides thermostable enzymes isolated from Caldicellulosiruptor bescii and fragments thereof useful for the degradation of cellulose and/or hemicellulose, including thermostable cellulases and hemicellulases. The disclosure further provides nucleic acids encoding the thermostable enzymes of the disclosure. The disclosure also provides methods for the conversion of cellulose and hemicellulose into fermentable sugars using thermostable enzymes of the disclosure. The disclosure also provides enzyme cocktails containing multiple enzymes disclosed herein. The enzymes can be used to release sugars present in cellulose or hemicellulose for subsequent fermentation to produce value-added products.
    Type: Application
    Filed: December 20, 2011
    Publication date: April 2, 2015
    Applicant: The Board pf Trustees of the University of Illinois
    Inventors: Yejun Han, Xiaoyun Su, Dylan Dodd, Roderick I. Mackie, Issac K.O. Cann
  • Publication number: 20150079659
    Abstract: The present invention provides a cell culture medium formulation that supports the in vitro cultivation, particularly in suspension, of mammalian cells, particularly epithelial cells and fibroblast cells, and methods for cultivating mammalian cells in suspension in vitro using these media. The media comprise a basal medium and a polyanionic or polyanionic compound, preferably a polysulfonated or polysulfated compound, and more preferably dextran sulfate. The present invention also provides chemically defined, protein-free eukaryotic cell culture media comprising an iron chelate and zinc, which is capable of supporting the growth (and particularly the high-density growth of mammalian cells) in suspension culture, increasing the level of expression of recombinant protein in cultured cells, and/or increasing virus production in cultured cells.
    Type: Application
    Filed: May 22, 2014
    Publication date: March 19, 2015
    Applicant: LIFE TECHNOLOGIES CORPORATION
    Inventors: Stephen GORFIEN, Richard FIKE, GLENN GODWIN, JOYCE WOLANSKE, David EPSTEIN, Dale GRUBER, Don MCCLURE, Paul PRICE
  • Patent number: 8980578
    Abstract: The present invention provides fungal xylanase and/or beta-xylosidase enzymes suitable for use in saccharification reactions. The present application further provides genetically modified fungal organisms that produce xylanase and/or beta-xylosidases, as well as enzyme mixtures exhibiting enhanced hydrolysis of cellulosic material to fermentable sugars, enzyme mixtures produced by the genetically modified fungal organisms, and methods for producing fermentable sugars from cellulose using such enzyme mixtures.
    Type: Grant
    Filed: June 10, 2013
    Date of Patent: March 17, 2015
    Assignee: Codexis, Inc.
    Inventors: Ryan Fong, Xiyun Zhang, Chris Noriega, Nicholas Agard, Anupam Gohel, Derek Smith
  • Patent number: 8980614
    Abstract: Methicillin-resistant (MRSA) and multi-drug resistant strains of Staphylococcus aureus are becoming increasingly prevalent in both human and veterinary clinics. S. aureus-causing bovine mastitis yields high annual losses to the dairy industry. Treatment of mastitis by broad range antibiotics is often not successful and may contribute to development of antibiotic resistance. Bacteriophage endolysins are a promising new source of antimicrobials. The endolysin of prophage ?SH2 of Staphylococcus haemolyticus strain JCSC1435 (?SH2 lysin) shows lytic activity against live staphylococcal cells. Deletion constructs were tested in zymograms and turbidity reduction assays to evaluate the contribution of each functional module to lysis. The CHAP domain exhibited three-fold higher activity than the full length protein. Activity was further enhanced in the presence of bivalent calcium ions.
    Type: Grant
    Filed: September 6, 2012
    Date of Patent: March 17, 2015
    Assignee: The United States of America, as represented by The Secretary of Agriculture
    Inventors: David M. Donovan, Igor V. Abaev, Mathias Schmelcher