Glucoamylase (3.2.1.3) Patents (Class 435/205)
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Patent number: 11319013Abstract: A straddle vehicle includes: a driving electric motor configured to generate drive power to be transmitted to a drive wheel; a seat on which a rider sits; a battery pack disposed under the seat and storing electric power to be supplied to the driving electric motor; and a vehicle body frame including a receiving base supporting the battery pack. The receiving base has five surfaces supporting front, rear, left, right, and bottom surfaces of the battery pack, respectively.Type: GrantFiled: March 25, 2020Date of Patent: May 3, 2022Assignee: KAWASAKI JUKOGYO KABUSHIKI KAISHAInventor: Shusei Ueno
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Patent number: 10800999Abstract: The present invention relates to method for production of brewer's wort comprising adding to a mash, a particular glucoamylase. The glucoamylase is obtained from Penicillium oxalicum. Further, the invention relates to use of a particular glucoamylase for production of brewer's wort. Furthermore, the invention relates to use of a combination of a glucoamylase and a pullulanase for production of brewer's wort.Type: GrantFiled: April 11, 2012Date of Patent: October 13, 2020Assignee: Novozymes A/SInventors: Anne Mette Bhatia Frederiksen, Shiro Fukuyama, Keiichi Ayabe
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Patent number: 10655112Abstract: A polypeptide includes an amino acid sequence selected from the group consisting of: an amino acid sequence having at least 85% sequence identity to the amino acid sequence of SEQ ID NO: 1; and an amino acid sequence derived from the amino acid sequence of SEQ ID NO: 1 by substitution, deletion, and/or addition of one or more amino acid residues. A yeast host expressing the polypeptide in a secretory production system does not add an N-linked sugar chain to the polypeptide, and the polypeptide has endonuclease activity.Type: GrantFiled: September 28, 2018Date of Patent: May 19, 2020Assignee: KANEKA CORPORATIONInventor: Tozo Nishiyama
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Patent number: 10344288Abstract: Genetically engineered yeast with a heterologous glucoamylase and fermentation methods are described. The engineered yeast can have multiple exogenous nucleic acid sequences which each have a different sequence, but that encode the same or a similar glucoamylase protein that is heterologous to the yeast. The engineered yeast exhibit desirable bioproduct production profiles during a fermentation process. A fermentation medium with a starch material can be fermented with the engineered yeast to provide high ethanol titers, low glycerol titers, or both.Type: GrantFiled: March 25, 2016Date of Patent: July 9, 2019Assignee: CARGILL, INCORPORATEDInventors: Christopher K. Miller, Ana Negrete-Raymond, Jon Veldhouse, Amit Vas
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Patent number: 10221405Abstract: The present invention relates to isolated polypeptides having amylolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: GrantFiled: October 20, 2016Date of Patent: March 5, 2019Assignee: NOVOZYMES, INC.Inventors: Paul Harris, Mark Wogulis
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Patent number: 9650620Abstract: Provided are isolated polypeptides having glucoamylase activity, catalytic domains, and polynucleotides encoding the polypeptides, catalytic domains. Also provided are nucleic acid constructs, vectors and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains.Type: GrantFiled: August 24, 2012Date of Patent: May 16, 2017Assignee: NOVOZYMES A/SInventors: Tianqi Sun, Ming Li
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Patent number: 9617527Abstract: The present invention relates to isolated polypeptides having glucoamylase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: GrantFiled: April 11, 2011Date of Patent: April 11, 2017Assignee: Novozymes A/SInventors: Ming Li, Junxin Duan, Zheng Liu, Shiro Fukuyama, Keiichi Ayabe, Guillermo Coward-Kelly, Randall Deinhammer
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Patent number: 9506046Abstract: The present invention relates to isolated polypeptides having amylolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: GrantFiled: November 2, 2009Date of Patent: November 29, 2016Assignee: Novozymes, Inc.Inventors: Paul Harris, Mark Wogulis
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Patent number: 9249400Abstract: In one aspect, the invention is directed to polypeptides having an amylase activity, polynucleotides encoding the polypeptides, and methods for making and using these polynucleotides and polypeptides. The polypeptides of the invention can be used as amylases to catalyze the hydrolysis of starch into sugars.Type: GrantFiled: August 28, 2013Date of Patent: February 2, 2016Assignee: BASF Enzymes LLCInventors: Kevin A. Gray, Nahla M. Aboushadi, James B. Garrett
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Publication number: 20150139977Abstract: The invention relates to enzymes having xylanase, mannanase and/or glucanase activity, e.g., catalyzing hydrolysis of internal ?-1,4-xylosidic linkages or endo-?-1,4-glucanase linkages; and/or degrading a linear polysaccharide beta-1,4-xylan into xylose. Thus, the invention provides methods and processes for breaking down hemicellulose, which is a major component of the cell wall of plants, including methods and processes for hydrolyzing hemicelluloses in any plant or wood or wood product, wood waste, paper pulp, paper product or paper waste or byproduct. In addition, methods of designing new xylanases, mannanases and/or glucanases and methods of use thereof are also provided. The xylanases, mannanases and/or glucanases have increased activity and stability at increased pH and temperature.Type: ApplicationFiled: October 22, 2014Publication date: May 21, 2015Inventors: David Weiner, David Blum, Alexander Varvak, Shaun Healey, Kristine Chang, Geoff Hazlewood, Thomas Todaro, Grace Desantis, Hwai Chang, Connie Jo Hansen, Scott W. Beaver, Thomas Woodward, Charles Hancock
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Publication number: 20150141680Abstract: The present invention provides compositions and methods related to the production and use of enzymes suitable for reducing the amount of steryl glycosides or saturated monoacyl glycerols in a lipid mixture.Type: ApplicationFiled: March 14, 2013Publication date: May 21, 2015Applicant: KECLON S.A.Inventors: Hugo Menzella, Salvador Peiru, Leandro Vetcher
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Patent number: 9029116Abstract: The present invention relates to glucoamylase variants. In particular, the invention relates to variants in the starch binding domain (SBD) of a glucoamylase. The invention also relates to variants having altered properties (e.g., improved thermostability and/or increased specific activity) as compared to a corresponding parent glucoamylase. The present invention also provides enzyme compositions comprising the variant glucoamylases; DNA constructs comprising polynucleotides encoding the variants; and methods of producing the glucoamylase variants in host cells.Type: GrantFiled: August 30, 2013Date of Patent: May 12, 2015Assignee: Danisco US Inc.Inventors: Wolfgang Aehle, Richard R. Bott, Igor Nikoaev, Martijn Scheffers, Piet Van Solingen, Casper Vroemen
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Publication number: 20150125902Abstract: Provided are isolated polypeptides having glucoamylase activity, catalytic domains, and polynucleotides encoding the polypeptides, catalytic domains. Also provided are nucleic acid constructs, vectors and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains.Type: ApplicationFiled: August 24, 2012Publication date: May 7, 2015Applicant: Novozymes A/SInventors: Tianqi Sun, Ming Li
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Publication number: 20150093792Abstract: The present invention relates to isolated polypeptides having glucoamylase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: November 24, 2014Publication date: April 2, 2015Inventors: Sara Landvik, Marc Dominique Morant, Keiichi Ayabe, Guillermo Coward-Kelly
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Patent number: 8975056Abstract: Alpha-amylases from Bacillus subtilis (AmyE), variants thereof, nucleic acids encoding the same, and host cells comprising the nucleic acids are provided. Methods of using AmyE or variants thereof are disclosed, including liquefaction and/or saccharification of starch. Such methods may yield sugars useful for ethanol production or high fructose corn syrup production. In some cases, the amylases can be used at low pH, in the absence of calcium, and/or in the absence of a glucoamylase.Type: GrantFiled: October 25, 2012Date of Patent: March 10, 2015Assignee: Danisco US Inc.Inventors: Luis G. Cascao-Pereira, William A. Cuevas, David A. Estell, Sang-Kyu Lee, Scott D. Power, Sandra W. Ramer, Amr Toppozada, Louise Wallace
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Publication number: 20150031091Abstract: The present invention provides isolated polypeptides having alpha-amylase activity catalytic domains, carbohydrate binding domains and polynucleotide encoding the polypeptides, catalytic domains or carbohydrate binding domains. The invention also provides nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains or carbohydrate binding domains.Type: ApplicationFiled: September 29, 2012Publication date: January 29, 2015Applicant: NOVOZYMES A/SInventors: Ming Li, Junxin Duan, Noriko Tsutsumi, Guillermo Coward-Kelly, Henrik Lundkvist
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Publication number: 20150010981Abstract: The present invention provides various GH61 protein variants comprising various amino acid substitutions. The GH61 protein variants have an improved ability to synergize with cellulase enzymes, thereby increasing the yield of fermentable sugars obtained by saccharification of biomass. In some embodiments, sugars obtained from saccharification are fermented to produce numerous end-products, including but not limited to alcohol.Type: ApplicationFiled: September 25, 2014Publication date: January 8, 2015Inventors: Jie Yang, Xiyun Zhang, Jungjoo Yoon, Kripa K. Rao, John H. Grate, David M. Elgart, Dipnath Baidyaroy
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Publication number: 20140356922Abstract: The present invention relates to polypeptides having glucoamylase activity with improved properties and to compositions comprising these polypeptides suitable for use in the production of a food, beverage (e.g. beer), feed, or biofuel. Also described is an improved and cost-effective process for isolating glucoamylases suitable for large scale protein purification procedures. Furthermore, different methods and uses related to glucoamylases according to the invention are disclosed, such as in a brewing process.Type: ApplicationFiled: December 20, 2012Publication date: December 4, 2014Inventors: Jacob Flyvholm Cramer, Neville Marshall Fish, Peter Edvard Degn, Igor Nikolaev, Paulien Kruithof, Piet Van Solingen, Sander Van Stigt Thans, Suzy Breneman, Jayarama K. Shetty, Sung Ho Lee
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Publication number: 20140342422Abstract: The present invention relates to glucoamylase variants having reduced sensitivity to protease nicking. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.Type: ApplicationFiled: September 5, 2012Publication date: November 20, 2014Applicant: NOVOZYMES A/SInventors: Tomoko Matsui, Suzanne Clark
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Patent number: 8883456Abstract: The invention relates to a process of process of producing a fermentation product, comprising: liquefying a starch-containing material to dextrins with an alpha-amylase in the presence of a GH61 polypeptide; saccharifying the dextrins to a sugar with a glucoamylase; and fermenting the sugar using a fermenting organism.Type: GrantFiled: March 30, 2011Date of Patent: November 11, 2014Assignee: Novozymes North America, Inc.Inventors: Chee-Leong Soong, Jeremy Saunders
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Publication number: 20140308725Abstract: The present invention provides isolated polypeptides having glucoamylase activity, catalytic domains, and polynucleotides encoding the polypeptides, catalytic domains. The invention also provides nucleic acid constructs, vectors and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains.Type: ApplicationFiled: September 7, 2012Publication date: October 16, 2014Applicant: NOVOZYMES A/SInventors: Tianqi Sun, Ming Li
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Publication number: 20140295493Abstract: The present invention relates to isolated polypeptides having protease activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.Type: ApplicationFiled: April 1, 2014Publication date: October 2, 2014Inventors: Hiroaki Udagawa, Christian Isak Jorgensen
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Patent number: 8841091Abstract: The present invention relates to polypeptides comprising a carbohydrate-binding module amino acid sequence and an alpha-amylase amino acid sequence as well as to the application of such polypeptides.Type: GrantFiled: November 2, 2012Date of Patent: September 23, 2014Assignees: Novozymes Als, Novozymec North America Inc.Inventors: Shiro Fukuyama, Tomoko Matsui, Chee Leong Soong, Eric Allain, Anders Vikso Nielsen, Hiroaki Udagawa, Ye Liu, Junxin Duan, Wenping Wu, Lene Nonboe Andersen
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Publication number: 20140255997Abstract: The present invention relates to glucoamylase variants having improved thermostability. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.Type: ApplicationFiled: October 11, 2012Publication date: September 11, 2014Inventors: Esben Peter Friis, Leonardo De Maria, Jesper Vind, Thomas A. Poulsen, Allan Svendsen, Steffen Danielsen, Rolf T. Lenhard, Henrik Friis-Madsen, Lars K. Skov
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Patent number: 8809023Abstract: The present invention relates to combinatorial variants of a parent glucoamylase that have altered properties for reducing the synthesis of condensation products during hydrolysis of starch. Accordingly the variants of a parent glucoamylase are suitable such as for use within brewing and glucose syrup production. Also disclosed are DNA constructs encoding the variants and methods of producing the glucoamylase variants in host cells.Type: GrantFiled: August 18, 2010Date of Patent: August 19, 2014Assignee: Danisco US Inc.Inventors: Peter Edvard Degn, Richard Bott, Casper Willem Vroemen, Martijn Silvan Scheffers, Wolfgang Aehle
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Publication number: 20140227754Abstract: The present disclosure relates to variants of a parent glucoamylase having altered properties (e.g., improved thermostability and/or specific activity). In particular, the present disclosure provides compositions comprising the variant glucoamylases, including starch hydrolyzing compositions and cleaning compositions. The disclosure also relates to DNA constructs encoding the variants and methods of producing the glucoamylase variants in host cells.Type: ApplicationFiled: February 4, 2014Publication date: August 14, 2014Applicant: Danisco US Inc.Inventors: Wolfgang Aehle, Richard R. Bott, Martijn Scheffers, Pieter van Solingen, Casper Vroemen
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Publication number: 20140206035Abstract: Provided are isolated polypeptides having glucoamylase activity, catalytic domains, and polynucleotides encoding the polypeptides, catalytic domains. Also provided are nucleic acid constructs, vectors and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains.Type: ApplicationFiled: August 24, 2012Publication date: July 24, 2014Inventors: Tianqi Sun, Ming Li
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Patent number: 8785158Abstract: The invention relates to a method for dissolving cellulose e.g. for the production of regenerated cellulose products such as films, fibers, particles and the like. In said method, the cellulose material is dissolved using an enzymatic treatment, followed by a base treatment. The invention is also directed to a method for producing cellulose particles wherein cellulose dissolved as described above is sprayed or mixed into a regenerating solution for precipitating cellulose particles. Moreover, the invention relates to the use of said cellulose particles produced with this method as a filler and/or coating pigment of paper and board. The invention is also directed to methods for producing and coating paper and board.Type: GrantFiled: June 27, 2006Date of Patent: July 22, 2014Assignee: Sappi Netherlands Services B.V.Inventors: Kimmo Koivunen, Petri Silenius, Janne Laine, Tapani Vuorinen
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Patent number: 8785165Abstract: Embodiments of the present disclosure relate to a process for producing downstream products, such as high-density glucose syrups and high-glucose fermentation feedstock, from a starch-containing substrate without a pH adjustment before the saccharification step. The saccharification is effectively catalyzed by a glucoamylase at a pH in the range of 5.2 to 5.6.Type: GrantFiled: April 27, 2012Date of Patent: July 22, 2014Assignee: Danisco US Inc.Inventors: Sung Ho Lee, Jayarama K. Shetty, Paula Johanna Maria Teunissen
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Patent number: 8722369Abstract: The invention relates to a variant of a parent fungal glucoamylase, which exhibits altered properties, in particular improved thermal stability and/or increased specific activity.Type: GrantFiled: March 1, 2011Date of Patent: May 13, 2014Assignee: Novozymes A/SInventors: Bjarne Ronfeldt Nielsen, Allan Svendsen, Henrik Pedersen, Jesper Vind, Hanne Vang Hendriksen, Torben Peter Frandsen
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Publication number: 20140127754Abstract: Methods to convert lignocellulosic biomass to fermentable sugars with enzymes that degrade the lignocellulosic material are provided, as well as novel combinations of enzymes, including those that provide a synergistic release of sugars from plant biomass.Type: ApplicationFiled: November 5, 2012Publication date: May 8, 2014Applicant: DYADIC INTERNATIONAL, INC.Inventors: Marco A. Baez-Vasquez, Richard Burlingame, Jon K. Magnuson, Marion Bradford, Arkady Panteleimonovich Sinitsyn
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Patent number: 8697412Abstract: The present invention relates to isolated polypeptides having glucoamylase activity, catalytic domains, carbohydrate binding domains and polynucleotides encoding the polypeptides, catalytic domains or carbohydrate binding domains. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains or carbohydrate binding domains.Type: GrantFiled: November 28, 2012Date of Patent: April 15, 2014Assignee: Novozymes A/SInventors: Sara Landvik, Keiichi Ayabe, Guillermo Coward-Kelly
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Publication number: 20140087442Abstract: The present invention relates to glucoamylase variants. In particular, the invention relates to variants in the starch binding domain (SBD) of a glucoamylase. The invention also relates to variants having altered properties (e.g., improved thermostability and/or increased specific activity) as compared to a corresponding parent glucoamylase. The present invention also provides enzyme compositions comprising the variant glucoamylases; DNA constructs comprising polynucleotides encoding the variants; and methods of producing the glucoamylase variants in host cells.Type: ApplicationFiled: August 30, 2013Publication date: March 27, 2014Applicant: Danisco US Inc.Inventors: Wolfgang Aehle, Richard R. Bott, Igor Nikoaev, Martijn Scheffers, Piet Van Solingen, Casper Vroemen
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Publication number: 20140087405Abstract: The object is to provide an ?-glucosidase in which a transglycosylation activity predominates, and use thereof, and the like. A modified ?-glucosidase consisting of an amino acid sequence in which one or two or more of the amino acid(s) is selected from a group of specific amino acids.Type: ApplicationFiled: March 5, 2012Publication date: March 27, 2014Applicant: AMANO ENZYME INC.Inventor: Satoru Ishihara
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Patent number: 8679792Abstract: The present disclosure relates to variants of a parent glucoamylase having altered properties (e.g., improved thermostability and/or specific activity). In particular, the present disclosure provides compositions comprising the variant glucoamylases, including starch hydrolyzing compositions and cleaning compositions. The disclosure also relates to DNA constructs encoding the variants and methods of producing the glucoamylase variants in host cells.Type: GrantFiled: September 30, 2011Date of Patent: March 25, 2014Assignee: Danisco US Inc.Inventors: Wolfgang Aehle, Richard R. Bott, Martijn Scheffers, Piet Van Solingen, Casper Vroemen
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Patent number: 8679815Abstract: The present invention relates to filamentous fungal host cells and particularly Trichoderma host cells useful for the production of heterologous granular starch hydrolyzing enzymes having glucoamylase activity.Type: GrantFiled: December 19, 2007Date of Patent: March 25, 2014Assignee: Danisco US Inc.Inventors: Toby M. Baldwin, Benjamin S. Bower, Nigel Dunn-Coleman, Suzanne E. Lantz, Michael J. Pepsin
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Publication number: 20140051119Abstract: The present invention provides fungal xylanase and/or beta-xylosidase enzymes suitable for use in saccharification reactions. The present application further provides genetically modified fungal organisms that produce xylanase and/or beta-xylosidases, as well as enzyme mixtures exhibiting enhanced hydrolysis of cellulosic material to fermentable sugars, enzyme mixtures produced by the genetically modified fungal organisms, and methods for producing fermentable sugars from cellulose using such enzyme mixtures.Type: ApplicationFiled: June 10, 2013Publication date: February 20, 2014Applicant: Codexis, Inc.Inventors: Ryan Fong, Xiyun Zhang, Chirs Noriega, Nicholas Agard, Anupam Gohel, Derek Smith
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Publication number: 20140045223Abstract: The present invention relates to isolated polypeptides having isoamylase activity derived from Dyella japonica and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. The invention also relates to the use of said polypeptide having isoamylase activity for producing glucose syrup, fructose syrup, maltose syrup or maltitol.Type: ApplicationFiled: October 11, 2013Publication date: February 13, 2014Applicant: Novozymes A/SInventors: Tine Hoff, Carsten Sjoeholm, Barrie Edmund Norman
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Publication number: 20140037790Abstract: The present invention is intended to provide a quality improving agent useful for improving the quality of steamed buns, and a quality improving agent containing glucoamylase. The quality improving agent preferably has low protease activity.Type: ApplicationFiled: February 20, 2012Publication date: February 6, 2014Applicant: AMANO ENZYME INC.Inventors: Qinglong Xu, Kimihiko Sato, Shotaro Yamaguchi
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Publication number: 20140038242Abstract: The present invention relates to isolated polypeptides having isoamylase activity derived from Dyella japonica and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. The invention also relates to the use of said polypeptide having isoamylase activity for producing glucose syrup, fructose syrup, maltose syrup or maltitol.Type: ApplicationFiled: October 11, 2013Publication date: February 6, 2014Applicant: NOVOZYMES A/SInventors: Tine Hoff, Carsten Sjoeholm, Barrie Edmund Norman
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Publication number: 20140007301Abstract: The present invention relates to isolated polypeptides having glucoamylase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: November 30, 2010Publication date: January 2, 2014Applicants: NOVOZYMES A/S, NOVOZYMES NORTH AMERICA, INCInventors: Marc D. Morant, Mikako Sasa, Keiichi Ayabe, Guillermo Coward-Kelly
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Publication number: 20130333072Abstract: The present invention relates to isolated polypeptides having glucoamylase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: November 30, 2010Publication date: December 12, 2013Applicants: Novozymes North America, Inc., Novozymes A/SInventors: Sara Landvik, Keiichi Ayabe, Guillermo Coward-Kelly
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Patent number: 8592194Abstract: The present invention relates to variant glucoamylases wherein the variant has altered properties (e.g., improved thermostability and/or specific activity) compared to a corresponding parent glucoamylase. The present invention also relates to enzyme compositions comprising a variant glucoamylase (e.g., starch hydrolyzing compositions); DNA constructs comprising polynucleotides encoding the variants and methods of producing the variant glucoamylases in host cells.Type: GrantFiled: April 8, 2008Date of Patent: November 26, 2013Assignee: Danisco US Inc.Inventors: Wolfgang Aehle, Richard R. Bott, Igor Nikolaev, Martijn Scheffers, Piet Van Solingen, Casper Vroemen
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Patent number: 8591969Abstract: The present invention relates to alpha-amylases, nucleic acids encoding the alpha-amylases, methods of producing the alpha-amylases, and methods of using the alpha-amylases.Type: GrantFiled: April 5, 2013Date of Patent: November 26, 2013Assignee: Novozymes A/SInventors: Carsten Andersen, Thomas Agersten Poulsen
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Publication number: 20130309726Abstract: The present invention relates to combinatorial variants of a parent glucoamylase that have altered properties for reducing the synthesis of condensation products during hydrolysis of starch. Accordingly the variants of a parent glucoamylase are suitable such as for use within brewing and glucose syrup production. Also disclosed are DNA constructs encoding the variants and methods of producing the glucoamylase variants in host cells.Type: ApplicationFiled: June 30, 2011Publication date: November 21, 2013Applicant: DUPONT NUTRITION BIOSCIENCES APSInventors: Peter Edvard Degn, Richard R. Bott, Casper Willem Vroemen, Martijn Silvan Scheffers, Wolfgang Aehle, Elin Petersen
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Publication number: 20130273611Abstract: Compositions and methods are provided for treating lignocellulosic material with a xylanase enzyme having xylanase activity. The enzyme is stable and active at increased pHs and temperatures. The present invention therefore provides methods for hydrolyzing lignocellulosic material, especially cellulose and hemicellulose, which are major components of the cell wall of non-woody and woody plants. The methods for hydrolyzing cellulose and hemicellulose can be used on any plant, wood or wood product, wood waste, paper pulp, paper product or paper waste or byproduct.Type: ApplicationFiled: September 8, 2011Publication date: October 17, 2013Applicant: SYNGENTA BIOTECHNOLOGY INC.Inventors: John Steffens, Paul Oeller, Yoshimi Barron
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Patent number: 8557541Abstract: The present invention relates to isolated polypeptides having glucoamylase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: GrantFiled: November 30, 2010Date of Patent: October 15, 2013Assignees: Novozymes A/S, Novozymes North America, IncInventors: Sara Landvik, Marc D. Morant, Keiichi Ayabe, Guillermo Coward-Kelly
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Publication number: 20130267002Abstract: The present invention relates to polypeptides having glucoamylase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: June 12, 2013Publication date: October 10, 2013Inventors: Sara Landvik, Marc Dominique Morant, Keiichi Ayabe, Guillermo Coward-Kelly
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Patent number: 8551755Abstract: The present invention relates to glucoamylase variants. In particular, the invention relates to variants in the starch binding domain (SBD) of a glucoamylase. The invention also relates to variants having altered properties (e.g., improved thermostability and/or increased specific activity) as compared to a corresponding parent glucoamylase. The present invention also provides enzyme compositions comprising the variant glucoamylases; DNA constructs comprising polynucleotides encoding the variants; and methods of producing the glucoamylase variants in host cells.Type: GrantFiled: April 8, 2008Date of Patent: October 8, 2013Assignee: Danisco US Inc.Inventors: Wolfgang Aehle, Richard R. Bott, Igor Nikolaev, Martijn Scheffers, Piet Van Solingen, Casper Vroemen
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Patent number: 8512986Abstract: The present invention relates to polypeptides comprising a carbohydrate-binding module amino acid sequence and an alpha-amylase amino acid sequence as well as to the application of such polypeptides.Type: GrantFiled: December 20, 2010Date of Patent: August 20, 2013Assignees: Novozymes A/S, Novozymes North America, Inc.Inventors: Shiro Fukuyama, Tomoko Matsui, Chee Leong Soong, Eric Allain, Anders Vikso Nielsen, Hiroaki Udagawa, Ye Liu, Junxin Duan, Wenping Wu, Lene Nonboe Andersen, Sara Landvik