Glucoamylase (3.2.1.3) Patents (Class 435/205)
  • Patent number: 11319013
    Abstract: A straddle vehicle includes: a driving electric motor configured to generate drive power to be transmitted to a drive wheel; a seat on which a rider sits; a battery pack disposed under the seat and storing electric power to be supplied to the driving electric motor; and a vehicle body frame including a receiving base supporting the battery pack. The receiving base has five surfaces supporting front, rear, left, right, and bottom surfaces of the battery pack, respectively.
    Type: Grant
    Filed: March 25, 2020
    Date of Patent: May 3, 2022
    Assignee: KAWASAKI JUKOGYO KABUSHIKI KAISHA
    Inventor: Shusei Ueno
  • Patent number: 10800999
    Abstract: The present invention relates to method for production of brewer's wort comprising adding to a mash, a particular glucoamylase. The glucoamylase is obtained from Penicillium oxalicum. Further, the invention relates to use of a particular glucoamylase for production of brewer's wort. Furthermore, the invention relates to use of a combination of a glucoamylase and a pullulanase for production of brewer's wort.
    Type: Grant
    Filed: April 11, 2012
    Date of Patent: October 13, 2020
    Assignee: Novozymes A/S
    Inventors: Anne Mette Bhatia Frederiksen, Shiro Fukuyama, Keiichi Ayabe
  • Patent number: 10655112
    Abstract: A polypeptide includes an amino acid sequence selected from the group consisting of: an amino acid sequence having at least 85% sequence identity to the amino acid sequence of SEQ ID NO: 1; and an amino acid sequence derived from the amino acid sequence of SEQ ID NO: 1 by substitution, deletion, and/or addition of one or more amino acid residues. A yeast host expressing the polypeptide in a secretory production system does not add an N-linked sugar chain to the polypeptide, and the polypeptide has endonuclease activity.
    Type: Grant
    Filed: September 28, 2018
    Date of Patent: May 19, 2020
    Assignee: KANEKA CORPORATION
    Inventor: Tozo Nishiyama
  • Patent number: 10344288
    Abstract: Genetically engineered yeast with a heterologous glucoamylase and fermentation methods are described. The engineered yeast can have multiple exogenous nucleic acid sequences which each have a different sequence, but that encode the same or a similar glucoamylase protein that is heterologous to the yeast. The engineered yeast exhibit desirable bioproduct production profiles during a fermentation process. A fermentation medium with a starch material can be fermented with the engineered yeast to provide high ethanol titers, low glycerol titers, or both.
    Type: Grant
    Filed: March 25, 2016
    Date of Patent: July 9, 2019
    Assignee: CARGILL, INCORPORATED
    Inventors: Christopher K. Miller, Ana Negrete-Raymond, Jon Veldhouse, Amit Vas
  • Patent number: 10221405
    Abstract: The present invention relates to isolated polypeptides having amylolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Grant
    Filed: October 20, 2016
    Date of Patent: March 5, 2019
    Assignee: NOVOZYMES, INC.
    Inventors: Paul Harris, Mark Wogulis
  • Patent number: 9650620
    Abstract: Provided are isolated polypeptides having glucoamylase activity, catalytic domains, and polynucleotides encoding the polypeptides, catalytic domains. Also provided are nucleic acid constructs, vectors and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains.
    Type: Grant
    Filed: August 24, 2012
    Date of Patent: May 16, 2017
    Assignee: NOVOZYMES A/S
    Inventors: Tianqi Sun, Ming Li
  • Patent number: 9617527
    Abstract: The present invention relates to isolated polypeptides having glucoamylase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Grant
    Filed: April 11, 2011
    Date of Patent: April 11, 2017
    Assignee: Novozymes A/S
    Inventors: Ming Li, Junxin Duan, Zheng Liu, Shiro Fukuyama, Keiichi Ayabe, Guillermo Coward-Kelly, Randall Deinhammer
  • Patent number: 9506046
    Abstract: The present invention relates to isolated polypeptides having amylolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Grant
    Filed: November 2, 2009
    Date of Patent: November 29, 2016
    Assignee: Novozymes, Inc.
    Inventors: Paul Harris, Mark Wogulis
  • Patent number: 9249400
    Abstract: In one aspect, the invention is directed to polypeptides having an amylase activity, polynucleotides encoding the polypeptides, and methods for making and using these polynucleotides and polypeptides. The polypeptides of the invention can be used as amylases to catalyze the hydrolysis of starch into sugars.
    Type: Grant
    Filed: August 28, 2013
    Date of Patent: February 2, 2016
    Assignee: BASF Enzymes LLC
    Inventors: Kevin A. Gray, Nahla M. Aboushadi, James B. Garrett
  • Publication number: 20150139977
    Abstract: The invention relates to enzymes having xylanase, mannanase and/or glucanase activity, e.g., catalyzing hydrolysis of internal ?-1,4-xylosidic linkages or endo-?-1,4-glucanase linkages; and/or degrading a linear polysaccharide beta-1,4-xylan into xylose. Thus, the invention provides methods and processes for breaking down hemicellulose, which is a major component of the cell wall of plants, including methods and processes for hydrolyzing hemicelluloses in any plant or wood or wood product, wood waste, paper pulp, paper product or paper waste or byproduct. In addition, methods of designing new xylanases, mannanases and/or glucanases and methods of use thereof are also provided. The xylanases, mannanases and/or glucanases have increased activity and stability at increased pH and temperature.
    Type: Application
    Filed: October 22, 2014
    Publication date: May 21, 2015
    Inventors: David Weiner, David Blum, Alexander Varvak, Shaun Healey, Kristine Chang, Geoff Hazlewood, Thomas Todaro, Grace Desantis, Hwai Chang, Connie Jo Hansen, Scott W. Beaver, Thomas Woodward, Charles Hancock
  • Publication number: 20150141680
    Abstract: The present invention provides compositions and methods related to the production and use of enzymes suitable for reducing the amount of steryl glycosides or saturated monoacyl glycerols in a lipid mixture.
    Type: Application
    Filed: March 14, 2013
    Publication date: May 21, 2015
    Applicant: KECLON S.A.
    Inventors: Hugo Menzella, Salvador Peiru, Leandro Vetcher
  • Patent number: 9029116
    Abstract: The present invention relates to glucoamylase variants. In particular, the invention relates to variants in the starch binding domain (SBD) of a glucoamylase. The invention also relates to variants having altered properties (e.g., improved thermostability and/or increased specific activity) as compared to a corresponding parent glucoamylase. The present invention also provides enzyme compositions comprising the variant glucoamylases; DNA constructs comprising polynucleotides encoding the variants; and methods of producing the glucoamylase variants in host cells.
    Type: Grant
    Filed: August 30, 2013
    Date of Patent: May 12, 2015
    Assignee: Danisco US Inc.
    Inventors: Wolfgang Aehle, Richard R. Bott, Igor Nikoaev, Martijn Scheffers, Piet Van Solingen, Casper Vroemen
  • Publication number: 20150125902
    Abstract: Provided are isolated polypeptides having glucoamylase activity, catalytic domains, and polynucleotides encoding the polypeptides, catalytic domains. Also provided are nucleic acid constructs, vectors and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains.
    Type: Application
    Filed: August 24, 2012
    Publication date: May 7, 2015
    Applicant: Novozymes A/S
    Inventors: Tianqi Sun, Ming Li
  • Publication number: 20150093792
    Abstract: The present invention relates to isolated polypeptides having glucoamylase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Application
    Filed: November 24, 2014
    Publication date: April 2, 2015
    Inventors: Sara Landvik, Marc Dominique Morant, Keiichi Ayabe, Guillermo Coward-Kelly
  • Patent number: 8975056
    Abstract: Alpha-amylases from Bacillus subtilis (AmyE), variants thereof, nucleic acids encoding the same, and host cells comprising the nucleic acids are provided. Methods of using AmyE or variants thereof are disclosed, including liquefaction and/or saccharification of starch. Such methods may yield sugars useful for ethanol production or high fructose corn syrup production. In some cases, the amylases can be used at low pH, in the absence of calcium, and/or in the absence of a glucoamylase.
    Type: Grant
    Filed: October 25, 2012
    Date of Patent: March 10, 2015
    Assignee: Danisco US Inc.
    Inventors: Luis G. Cascao-Pereira, William A. Cuevas, David A. Estell, Sang-Kyu Lee, Scott D. Power, Sandra W. Ramer, Amr Toppozada, Louise Wallace
  • Publication number: 20150031091
    Abstract: The present invention provides isolated polypeptides having alpha-amylase activity catalytic domains, carbohydrate binding domains and polynucleotide encoding the polypeptides, catalytic domains or carbohydrate binding domains. The invention also provides nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains or carbohydrate binding domains.
    Type: Application
    Filed: September 29, 2012
    Publication date: January 29, 2015
    Applicant: NOVOZYMES A/S
    Inventors: Ming Li, Junxin Duan, Noriko Tsutsumi, Guillermo Coward-Kelly, Henrik Lundkvist
  • Publication number: 20150010981
    Abstract: The present invention provides various GH61 protein variants comprising various amino acid substitutions. The GH61 protein variants have an improved ability to synergize with cellulase enzymes, thereby increasing the yield of fermentable sugars obtained by saccharification of biomass. In some embodiments, sugars obtained from saccharification are fermented to produce numerous end-products, including but not limited to alcohol.
    Type: Application
    Filed: September 25, 2014
    Publication date: January 8, 2015
    Inventors: Jie Yang, Xiyun Zhang, Jungjoo Yoon, Kripa K. Rao, John H. Grate, David M. Elgart, Dipnath Baidyaroy
  • Publication number: 20140356922
    Abstract: The present invention relates to polypeptides having glucoamylase activity with improved properties and to compositions comprising these polypeptides suitable for use in the production of a food, beverage (e.g. beer), feed, or biofuel. Also described is an improved and cost-effective process for isolating glucoamylases suitable for large scale protein purification procedures. Furthermore, different methods and uses related to glucoamylases according to the invention are disclosed, such as in a brewing process.
    Type: Application
    Filed: December 20, 2012
    Publication date: December 4, 2014
    Inventors: Jacob Flyvholm Cramer, Neville Marshall Fish, Peter Edvard Degn, Igor Nikolaev, Paulien Kruithof, Piet Van Solingen, Sander Van Stigt Thans, Suzy Breneman, Jayarama K. Shetty, Sung Ho Lee
  • Publication number: 20140342422
    Abstract: The present invention relates to glucoamylase variants having reduced sensitivity to protease nicking. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
    Type: Application
    Filed: September 5, 2012
    Publication date: November 20, 2014
    Applicant: NOVOZYMES A/S
    Inventors: Tomoko Matsui, Suzanne Clark
  • Patent number: 8883456
    Abstract: The invention relates to a process of process of producing a fermentation product, comprising: liquefying a starch-containing material to dextrins with an alpha-amylase in the presence of a GH61 polypeptide; saccharifying the dextrins to a sugar with a glucoamylase; and fermenting the sugar using a fermenting organism.
    Type: Grant
    Filed: March 30, 2011
    Date of Patent: November 11, 2014
    Assignee: Novozymes North America, Inc.
    Inventors: Chee-Leong Soong, Jeremy Saunders
  • Publication number: 20140308725
    Abstract: The present invention provides isolated polypeptides having glucoamylase activity, catalytic domains, and polynucleotides encoding the polypeptides, catalytic domains. The invention also provides nucleic acid constructs, vectors and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains.
    Type: Application
    Filed: September 7, 2012
    Publication date: October 16, 2014
    Applicant: NOVOZYMES A/S
    Inventors: Tianqi Sun, Ming Li
  • Publication number: 20140295493
    Abstract: The present invention relates to isolated polypeptides having protease activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.
    Type: Application
    Filed: April 1, 2014
    Publication date: October 2, 2014
    Inventors: Hiroaki Udagawa, Christian Isak Jorgensen
  • Patent number: 8841091
    Abstract: The present invention relates to polypeptides comprising a carbohydrate-binding module amino acid sequence and an alpha-amylase amino acid sequence as well as to the application of such polypeptides.
    Type: Grant
    Filed: November 2, 2012
    Date of Patent: September 23, 2014
    Assignees: Novozymes Als, Novozymec North America Inc.
    Inventors: Shiro Fukuyama, Tomoko Matsui, Chee Leong Soong, Eric Allain, Anders Vikso Nielsen, Hiroaki Udagawa, Ye Liu, Junxin Duan, Wenping Wu, Lene Nonboe Andersen
  • Publication number: 20140255997
    Abstract: The present invention relates to glucoamylase variants having improved thermostability. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
    Type: Application
    Filed: October 11, 2012
    Publication date: September 11, 2014
    Inventors: Esben Peter Friis, Leonardo De Maria, Jesper Vind, Thomas A. Poulsen, Allan Svendsen, Steffen Danielsen, Rolf T. Lenhard, Henrik Friis-Madsen, Lars K. Skov
  • Patent number: 8809023
    Abstract: The present invention relates to combinatorial variants of a parent glucoamylase that have altered properties for reducing the synthesis of condensation products during hydrolysis of starch. Accordingly the variants of a parent glucoamylase are suitable such as for use within brewing and glucose syrup production. Also disclosed are DNA constructs encoding the variants and methods of producing the glucoamylase variants in host cells.
    Type: Grant
    Filed: August 18, 2010
    Date of Patent: August 19, 2014
    Assignee: Danisco US Inc.
    Inventors: Peter Edvard Degn, Richard Bott, Casper Willem Vroemen, Martijn Silvan Scheffers, Wolfgang Aehle
  • Publication number: 20140227754
    Abstract: The present disclosure relates to variants of a parent glucoamylase having altered properties (e.g., improved thermostability and/or specific activity). In particular, the present disclosure provides compositions comprising the variant glucoamylases, including starch hydrolyzing compositions and cleaning compositions. The disclosure also relates to DNA constructs encoding the variants and methods of producing the glucoamylase variants in host cells.
    Type: Application
    Filed: February 4, 2014
    Publication date: August 14, 2014
    Applicant: Danisco US Inc.
    Inventors: Wolfgang Aehle, Richard R. Bott, Martijn Scheffers, Pieter van Solingen, Casper Vroemen
  • Publication number: 20140206035
    Abstract: Provided are isolated polypeptides having glucoamylase activity, catalytic domains, and polynucleotides encoding the polypeptides, catalytic domains. Also provided are nucleic acid constructs, vectors and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains.
    Type: Application
    Filed: August 24, 2012
    Publication date: July 24, 2014
    Inventors: Tianqi Sun, Ming Li
  • Patent number: 8785158
    Abstract: The invention relates to a method for dissolving cellulose e.g. for the production of regenerated cellulose products such as films, fibers, particles and the like. In said method, the cellulose material is dissolved using an enzymatic treatment, followed by a base treatment. The invention is also directed to a method for producing cellulose particles wherein cellulose dissolved as described above is sprayed or mixed into a regenerating solution for precipitating cellulose particles. Moreover, the invention relates to the use of said cellulose particles produced with this method as a filler and/or coating pigment of paper and board. The invention is also directed to methods for producing and coating paper and board.
    Type: Grant
    Filed: June 27, 2006
    Date of Patent: July 22, 2014
    Assignee: Sappi Netherlands Services B.V.
    Inventors: Kimmo Koivunen, Petri Silenius, Janne Laine, Tapani Vuorinen
  • Patent number: 8785165
    Abstract: Embodiments of the present disclosure relate to a process for producing downstream products, such as high-density glucose syrups and high-glucose fermentation feedstock, from a starch-containing substrate without a pH adjustment before the saccharification step. The saccharification is effectively catalyzed by a glucoamylase at a pH in the range of 5.2 to 5.6.
    Type: Grant
    Filed: April 27, 2012
    Date of Patent: July 22, 2014
    Assignee: Danisco US Inc.
    Inventors: Sung Ho Lee, Jayarama K. Shetty, Paula Johanna Maria Teunissen
  • Patent number: 8722369
    Abstract: The invention relates to a variant of a parent fungal glucoamylase, which exhibits altered properties, in particular improved thermal stability and/or increased specific activity.
    Type: Grant
    Filed: March 1, 2011
    Date of Patent: May 13, 2014
    Assignee: Novozymes A/S
    Inventors: Bjarne Ronfeldt Nielsen, Allan Svendsen, Henrik Pedersen, Jesper Vind, Hanne Vang Hendriksen, Torben Peter Frandsen
  • Publication number: 20140127754
    Abstract: Methods to convert lignocellulosic biomass to fermentable sugars with enzymes that degrade the lignocellulosic material are provided, as well as novel combinations of enzymes, including those that provide a synergistic release of sugars from plant biomass.
    Type: Application
    Filed: November 5, 2012
    Publication date: May 8, 2014
    Applicant: DYADIC INTERNATIONAL, INC.
    Inventors: Marco A. Baez-Vasquez, Richard Burlingame, Jon K. Magnuson, Marion Bradford, Arkady Panteleimonovich Sinitsyn
  • Patent number: 8697412
    Abstract: The present invention relates to isolated polypeptides having glucoamylase activity, catalytic domains, carbohydrate binding domains and polynucleotides encoding the polypeptides, catalytic domains or carbohydrate binding domains. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains or carbohydrate binding domains.
    Type: Grant
    Filed: November 28, 2012
    Date of Patent: April 15, 2014
    Assignee: Novozymes A/S
    Inventors: Sara Landvik, Keiichi Ayabe, Guillermo Coward-Kelly
  • Publication number: 20140087442
    Abstract: The present invention relates to glucoamylase variants. In particular, the invention relates to variants in the starch binding domain (SBD) of a glucoamylase. The invention also relates to variants having altered properties (e.g., improved thermostability and/or increased specific activity) as compared to a corresponding parent glucoamylase. The present invention also provides enzyme compositions comprising the variant glucoamylases; DNA constructs comprising polynucleotides encoding the variants; and methods of producing the glucoamylase variants in host cells.
    Type: Application
    Filed: August 30, 2013
    Publication date: March 27, 2014
    Applicant: Danisco US Inc.
    Inventors: Wolfgang Aehle, Richard R. Bott, Igor Nikoaev, Martijn Scheffers, Piet Van Solingen, Casper Vroemen
  • Publication number: 20140087405
    Abstract: The object is to provide an ?-glucosidase in which a transglycosylation activity predominates, and use thereof, and the like. A modified ?-glucosidase consisting of an amino acid sequence in which one or two or more of the amino acid(s) is selected from a group of specific amino acids.
    Type: Application
    Filed: March 5, 2012
    Publication date: March 27, 2014
    Applicant: AMANO ENZYME INC.
    Inventor: Satoru Ishihara
  • Patent number: 8679792
    Abstract: The present disclosure relates to variants of a parent glucoamylase having altered properties (e.g., improved thermostability and/or specific activity). In particular, the present disclosure provides compositions comprising the variant glucoamylases, including starch hydrolyzing compositions and cleaning compositions. The disclosure also relates to DNA constructs encoding the variants and methods of producing the glucoamylase variants in host cells.
    Type: Grant
    Filed: September 30, 2011
    Date of Patent: March 25, 2014
    Assignee: Danisco US Inc.
    Inventors: Wolfgang Aehle, Richard R. Bott, Martijn Scheffers, Piet Van Solingen, Casper Vroemen
  • Patent number: 8679815
    Abstract: The present invention relates to filamentous fungal host cells and particularly Trichoderma host cells useful for the production of heterologous granular starch hydrolyzing enzymes having glucoamylase activity.
    Type: Grant
    Filed: December 19, 2007
    Date of Patent: March 25, 2014
    Assignee: Danisco US Inc.
    Inventors: Toby M. Baldwin, Benjamin S. Bower, Nigel Dunn-Coleman, Suzanne E. Lantz, Michael J. Pepsin
  • Publication number: 20140051119
    Abstract: The present invention provides fungal xylanase and/or beta-xylosidase enzymes suitable for use in saccharification reactions. The present application further provides genetically modified fungal organisms that produce xylanase and/or beta-xylosidases, as well as enzyme mixtures exhibiting enhanced hydrolysis of cellulosic material to fermentable sugars, enzyme mixtures produced by the genetically modified fungal organisms, and methods for producing fermentable sugars from cellulose using such enzyme mixtures.
    Type: Application
    Filed: June 10, 2013
    Publication date: February 20, 2014
    Applicant: Codexis, Inc.
    Inventors: Ryan Fong, Xiyun Zhang, Chirs Noriega, Nicholas Agard, Anupam Gohel, Derek Smith
  • Publication number: 20140045223
    Abstract: The present invention relates to isolated polypeptides having isoamylase activity derived from Dyella japonica and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. The invention also relates to the use of said polypeptide having isoamylase activity for producing glucose syrup, fructose syrup, maltose syrup or maltitol.
    Type: Application
    Filed: October 11, 2013
    Publication date: February 13, 2014
    Applicant: Novozymes A/S
    Inventors: Tine Hoff, Carsten Sjoeholm, Barrie Edmund Norman
  • Publication number: 20140037790
    Abstract: The present invention is intended to provide a quality improving agent useful for improving the quality of steamed buns, and a quality improving agent containing glucoamylase. The quality improving agent preferably has low protease activity.
    Type: Application
    Filed: February 20, 2012
    Publication date: February 6, 2014
    Applicant: AMANO ENZYME INC.
    Inventors: Qinglong Xu, Kimihiko Sato, Shotaro Yamaguchi
  • Publication number: 20140038242
    Abstract: The present invention relates to isolated polypeptides having isoamylase activity derived from Dyella japonica and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. The invention also relates to the use of said polypeptide having isoamylase activity for producing glucose syrup, fructose syrup, maltose syrup or maltitol.
    Type: Application
    Filed: October 11, 2013
    Publication date: February 6, 2014
    Applicant: NOVOZYMES A/S
    Inventors: Tine Hoff, Carsten Sjoeholm, Barrie Edmund Norman
  • Publication number: 20140007301
    Abstract: The present invention relates to isolated polypeptides having glucoamylase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Application
    Filed: November 30, 2010
    Publication date: January 2, 2014
    Applicants: NOVOZYMES A/S, NOVOZYMES NORTH AMERICA, INC
    Inventors: Marc D. Morant, Mikako Sasa, Keiichi Ayabe, Guillermo Coward-Kelly
  • Publication number: 20130333072
    Abstract: The present invention relates to isolated polypeptides having glucoamylase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Application
    Filed: November 30, 2010
    Publication date: December 12, 2013
    Applicants: Novozymes North America, Inc., Novozymes A/S
    Inventors: Sara Landvik, Keiichi Ayabe, Guillermo Coward-Kelly
  • Patent number: 8592194
    Abstract: The present invention relates to variant glucoamylases wherein the variant has altered properties (e.g., improved thermostability and/or specific activity) compared to a corresponding parent glucoamylase. The present invention also relates to enzyme compositions comprising a variant glucoamylase (e.g., starch hydrolyzing compositions); DNA constructs comprising polynucleotides encoding the variants and methods of producing the variant glucoamylases in host cells.
    Type: Grant
    Filed: April 8, 2008
    Date of Patent: November 26, 2013
    Assignee: Danisco US Inc.
    Inventors: Wolfgang Aehle, Richard R. Bott, Igor Nikolaev, Martijn Scheffers, Piet Van Solingen, Casper Vroemen
  • Patent number: 8591969
    Abstract: The present invention relates to alpha-amylases, nucleic acids encoding the alpha-amylases, methods of producing the alpha-amylases, and methods of using the alpha-amylases.
    Type: Grant
    Filed: April 5, 2013
    Date of Patent: November 26, 2013
    Assignee: Novozymes A/S
    Inventors: Carsten Andersen, Thomas Agersten Poulsen
  • Publication number: 20130309726
    Abstract: The present invention relates to combinatorial variants of a parent glucoamylase that have altered properties for reducing the synthesis of condensation products during hydrolysis of starch. Accordingly the variants of a parent glucoamylase are suitable such as for use within brewing and glucose syrup production. Also disclosed are DNA constructs encoding the variants and methods of producing the glucoamylase variants in host cells.
    Type: Application
    Filed: June 30, 2011
    Publication date: November 21, 2013
    Applicant: DUPONT NUTRITION BIOSCIENCES APS
    Inventors: Peter Edvard Degn, Richard R. Bott, Casper Willem Vroemen, Martijn Silvan Scheffers, Wolfgang Aehle, Elin Petersen
  • Publication number: 20130273611
    Abstract: Compositions and methods are provided for treating lignocellulosic material with a xylanase enzyme having xylanase activity. The enzyme is stable and active at increased pHs and temperatures. The present invention therefore provides methods for hydrolyzing lignocellulosic material, especially cellulose and hemicellulose, which are major components of the cell wall of non-woody and woody plants. The methods for hydrolyzing cellulose and hemicellulose can be used on any plant, wood or wood product, wood waste, paper pulp, paper product or paper waste or byproduct.
    Type: Application
    Filed: September 8, 2011
    Publication date: October 17, 2013
    Applicant: SYNGENTA BIOTECHNOLOGY INC.
    Inventors: John Steffens, Paul Oeller, Yoshimi Barron
  • Patent number: 8557541
    Abstract: The present invention relates to isolated polypeptides having glucoamylase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Grant
    Filed: November 30, 2010
    Date of Patent: October 15, 2013
    Assignees: Novozymes A/S, Novozymes North America, Inc
    Inventors: Sara Landvik, Marc D. Morant, Keiichi Ayabe, Guillermo Coward-Kelly
  • Publication number: 20130267002
    Abstract: The present invention relates to polypeptides having glucoamylase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Application
    Filed: June 12, 2013
    Publication date: October 10, 2013
    Inventors: Sara Landvik, Marc Dominique Morant, Keiichi Ayabe, Guillermo Coward-Kelly
  • Patent number: 8551755
    Abstract: The present invention relates to glucoamylase variants. In particular, the invention relates to variants in the starch binding domain (SBD) of a glucoamylase. The invention also relates to variants having altered properties (e.g., improved thermostability and/or increased specific activity) as compared to a corresponding parent glucoamylase. The present invention also provides enzyme compositions comprising the variant glucoamylases; DNA constructs comprising polynucleotides encoding the variants; and methods of producing the glucoamylase variants in host cells.
    Type: Grant
    Filed: April 8, 2008
    Date of Patent: October 8, 2013
    Assignee: Danisco US Inc.
    Inventors: Wolfgang Aehle, Richard R. Bott, Igor Nikolaev, Martijn Scheffers, Piet Van Solingen, Casper Vroemen
  • Patent number: 8512986
    Abstract: The present invention relates to polypeptides comprising a carbohydrate-binding module amino acid sequence and an alpha-amylase amino acid sequence as well as to the application of such polypeptides.
    Type: Grant
    Filed: December 20, 2010
    Date of Patent: August 20, 2013
    Assignees: Novozymes A/S, Novozymes North America, Inc.
    Inventors: Shiro Fukuyama, Tomoko Matsui, Chee Leong Soong, Eric Allain, Anders Vikso Nielsen, Hiroaki Udagawa, Ye Liu, Junxin Duan, Wenping Wu, Lene Nonboe Andersen, Sara Landvik