Acting On Beta-1, 4-glucosidic Bond (e.g., Cellulase, Etc. (3.2.1.4)) Patents (Class 435/209)
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Publication number: 20130007919Abstract: Intein modified proteins, isolated nucleic acids encoding intein modified proteins, fragments of intein modified proteins, isolated nucleic acids encoding fragments of intein modified proteins, transgenic plants containing any of the foregoing, and antibodies recognizing epitopes on intein modified proteins are providedType: ApplicationFiled: November 5, 2010Publication date: January 3, 2013Applicant: AGRIVIDA, INC.Inventors: Binzhang Shen, Gabor Lazar, Humberto de la Vega, Philip Lessard, R. Michael Raab, James Apgar
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Patent number: 8343907Abstract: Particulate bleach additive composition containing enzymes, which can be used to bleach fabrics in conjunction with a conventional granular or liquid laundry detergent.Type: GrantFiled: August 12, 2009Date of Patent: January 1, 2013Assignee: The Procter & Gamble CompanyInventors: Giulia Ottavia Bianchetti, Gloria Dicapua, Giovanni Grande, Neil Joseph Lant
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Publication number: 20120331588Abstract: The present invention relates to isolated polypeptides having endoglucanase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: August 30, 2012Publication date: December 27, 2012Applicant: Novozymes A/SInventors: Alfredo Lopez de Leon, Michael Rey
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Patent number: 8338121Abstract: The present invention relates to methods for determining cellulolytic enhancing activity of a polypeptide, comprising: (a) incubating a cellulosic material with an enzyme composition comprising a cellobiose dehydrogenase and one or more cellulolytic enzymes in the presence and absence of the polypeptide; and (b) measuring the release of sugar from the cellulosic material in the presence and absence of the polypeptide.Type: GrantFiled: December 17, 2009Date of Patent: December 25, 2012Assignee: Novozymes, Inc.Inventors: Matthew David Sweeney, Elena Vlasenko
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Publication number: 20120322106Abstract: A method for producing hemicellulase and cellulase for use in processing cellulosic materials for biofuel production. The method involves transforming algal feedstock to express hemicellulase and cellulase and then collecting hemicellulase and cellulase that has been secreted into a culture medium by the algae. The method may further involve transforming the algal feedstock to express other recombinant products and/or processing the alga feedstock to produce biofuels.Type: ApplicationFiled: February 18, 2011Publication date: December 20, 2012Inventor: Bobban Subhadra
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Publication number: 20120315685Abstract: Disclosed herein are coated articles and methods of preparing the same.Type: ApplicationFiled: August 20, 2012Publication date: December 13, 2012Applicant: ECOLOGY COATINGS, INC. A Nevada CorporationInventor: Sally Judith Weine Ramsey
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Publication number: 20120315676Abstract: The present invention relates to variant endoglucanases and particularly endoglucanases having improved properties over wild-type endoglucanase.Type: ApplicationFiled: June 11, 2012Publication date: December 13, 2012Applicant: Codexis, Inc.Inventors: Jie YANG, Andrew Shaw, Ish Kumar Dhawan, Onorato Campopiano, Kripa Rao
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Publication number: 20120316320Abstract: The presently disclosed subject matter relates to Managed Ecosystem Fermentation (MEF) which is a continuous microbial process utilizing a managed ecosystem approach employing dozens to thousands of species of microorganisms, occurring in a controlled artificial environment and consuming organic materials without benefit of sterilization. The process of utilizing this fermentation for the consumption of organic materials on a continuous basis is within the scope of this disclosed subject matter. The process of separating chemicals as industrial chemicals from this fermentation on a continuous basis is within the scope of this disclosed subject matter. The process of separating biomass useful as high protein animal feed or fertilizer from this fermentation on a continuous (or semi-continuous) basis is within the scope of this disclosed subject matter.Type: ApplicationFiled: June 11, 2012Publication date: December 13, 2012Inventors: Edward Arthur Calt, JR., Herbert Graham Tull, IV, Stanley Sylvester Toporek
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Publication number: 20120309059Abstract: The present invention relates to isolated polypeptides having endoglucanase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.Type: ApplicationFiled: July 31, 2012Publication date: December 6, 2012Applicants: NOVOZYMES, INC., NOVOZYMES A/SInventors: Paul Harris, Elena Vlasenko, Elizabeth Zaretsky, Marcus Sakari Kauppinnen, Sarah Teter, Kimberly Brown
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Patent number: 8323947Abstract: The invention relates to recombinant expression of a variant form of a fungal C1 strain ?-glucosidase. The invention also relates to the generation of fermentable sugars from biomass and the production of biofuels by fermentation of the sugars using genetically modified organisms expressing the ?-glucosidase variant. The invention provides methods for producing a fermentable sugar, such as glucose, from cellobiose by contacting cellobiose with a recombinant ?-glucosidase variant protein, such as a variant protein secreted by a recombinant host cell into culture medium. Methods of the invention may be used for conversion of a biomass substrate to a fermentable sugar, and ultimately to ethanol or other biofuel.Type: GrantFiled: March 9, 2012Date of Patent: December 4, 2012Assignee: Codexis, Inc.Inventors: Jie Yang, Xiyun Zhang, Attila Andor
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Patent number: 8323931Abstract: A fermentation process for the production of cellulase mixtures is provided. The process comprises providing a genetically modified host filamentous fungus that overexpresses a Xyr1 transcription factor and/or that is partially or completely deficient in expressing one or more hemicellulase enzyme. The host filamentous fungus is cultured in a medium comprising a carbon source. The carbon source contains from about 60 wt % to about 100 wt % hemicellulose-derived carbohydrate and less than 5% of a cellulase-inducing carbohydrate or contains from about 25 wt % to about 100% wt % hemicellulose-derived sugar alcohol in combination with from about 0 wt % to about 75 wt % glucose, glycerol or a combination thereof.Type: GrantFiled: November 3, 2009Date of Patent: December 4, 2012Assignee: Iogen Energy CorporationInventors: Loreta Gudynaite-Savitch, Christopher D. Hindle, Theresa C. White
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Publication number: 20120301944Abstract: The present invention provides a novel ?-glucosidase nucleic acid sequence, designated bgl6, and the corresponding BGL6 amino acid sequence. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding BGL6, recombinant BGL6 proteins and methods for producing the same.Type: ApplicationFiled: April 25, 2012Publication date: November 29, 2012Applicant: Danisco US Inc.Inventors: Nigel Dunn-Coleman, Michael Ward
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Patent number: 8318461Abstract: A process for the enzymatic hydrolysis of cellulose to produce a hydrolysis product comprising glucose from a pretreated lignocellulosic feedstock and enzymes for use in the process are provided. The process comprises hydrolyzing an aqueous slurry of a pretreated lignocellulosic feedstock with cellulase enzymes, one or more than one ?-glucosidase enzyme and a binding agent for binding the ?-glucosidase enzyme to fiber solids present in the aqueous slurry. During the hydrolysis, both the cellulase enzyme and ?-glucosidase enzyme bind to the fiber solids. The hydrolysis is performed in a solids-retaining hydrolysis reactor so that unhydrolyzed fiber solids and bound enzyme are retained in the reactor longer than the aqueous phase of the slurry.Type: GrantFiled: June 22, 2007Date of Patent: November 27, 2012Assignee: Iogen Energy CorporationInventors: Jeffrey Tolan, Theresa White, John Tomashek
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Patent number: 8318436Abstract: The present invention provides a method and compositions for high throughput screening of genetically modified photosynthetic organisms for plasmic state. The present invention provides methods of producing one or more proteins, including biomass degrading enzymes in a plant. Also provided are the methods of producing biomass degradation pathways in alga cells, particularly in the chloroplast. Single enzymes or multiple enzymes may be produced by the methods disclosed. The methods disclosed herein allow for the production of biofuel, including ethanol.Type: GrantFiled: October 7, 2011Date of Patent: November 27, 2012Assignees: Sapphire Energy, Inc., The Scripps Research InstituteInventors: Stephen Mayfield, Bryan O'Neill, Michael Mendez, Yan Poon
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Publication number: 20120295308Abstract: Provided is a modified Family 1 carbohydrate binding module (CBM) comprising amino acid substitutions at one or more of positions 10, 11, 12, 14, 17, 21, 24, 29, 31, 33, and 37, said position determined from alignment of a Family 1 CBM amino acid sequence with SEQ ID NO: 30, and exhibiting from about 50% to about 99.9% amino acid sequence identity to SEQ ID NO: 30. Also provided are modified glycosidase enzymes comprising the modified Family 1 CBM, genetic constructs and genetically modified microbes for expressing the modified Family 1 CBM or modified glycosidase enzyme. The modified Family 1 CBM confers reduced lignin binding and/or increased hydrolysing activity in the presence of lignin to the modified glycosidase enzyme, which may be used in a process for hydrolysing cellulose or hemicellulose in the presence of lignin.Type: ApplicationFiled: February 11, 2011Publication date: November 22, 2012Applicant: IOGEN ENEGY CORPORATIONInventors: John J. Tomashek, Brian R. Scott, Daniel Kolczynski
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Publication number: 20120289450Abstract: The present invention relates to cellulase variants, i.e., endo-beta-1,4-glucanase variants, derived from a parental cellulase, i.e., endo-beta-1,4-glucanase, by substitution, insertion and/or deletion, which variant has a catalytic core domain, in which the variant at position 5 holds an alanine residue (A), a serine residue (S), or a threonine residue (T); at position 8 holds a phenylalanine residue (F), or a tyrosine residue (Y); at position 9 holds a phenylalanine residue (F), a tryptophan residue (W), or a tyrosine residue (Y); at position 10 holds an aspartic acid residue (D); and at position 121 holds an aspartic acid residue (D).Type: ApplicationFiled: May 15, 2012Publication date: November 15, 2012Applicant: NOVOZYMES A/SInventors: Kim Vilbour Andersen, Martin Schülein, Lars Christiansen, Bo Damgaard, Claus Von der Osten
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Patent number: 8309338Abstract: The present invention relates to isolated polypeptides having endoglucanase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.Type: GrantFiled: April 25, 2011Date of Patent: November 13, 2012Assignee: Novozymes A/SInventors: Katja Salomon Johansen, Keith Gibson, Preben Nielsen, Helle Outtrup
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Publication number: 20120276640Abstract: The present invention relates to isolated polypeptides having cellobiohydrolase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: July 13, 2012Publication date: November 1, 2012Applicant: Novozymes A/SInventors: Suchindra Maiyuran, Paul Harris, Hanshu Ding, Kimberly Brown
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Publication number: 20120276595Abstract: The present disclosure relates to cellulase variants. In particular the present disclosure relates to cellulase variants having reduced binding to non-cellulosic materials. Also described are nucleic acids encoding the cellulase, compositions comprising said cellulase, methods of identifying cellulose variants and methods of using the compositions.Type: ApplicationFiled: July 6, 2012Publication date: November 1, 2012Applicant: DANISCO US INC.Inventors: Luis G. Cascao-Pereira, Thijs Kaper, Bradley R. Kelemen, Amy D. Liu
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Publication number: 20120276594Abstract: The present invention relates to cellobiohydrolase variants having improved thermostability and/or thermoactivity in comparison to wild-type Myceliophthora thermophila CBH2b.Type: ApplicationFiled: April 27, 2012Publication date: November 1, 2012Applicant: Codexis, Inc.Inventors: Rama Voladri, Xiyun Zhang, Sachin Patil, David Elgart, Gregory Miller, Louis Clark, Kui Chan
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Publication number: 20120276567Abstract: The invention relates to a Talaromyces transformant comprising one or more recombinant gene, capable of producing cellulase in the absence of cellulase inducer in a glucose medium, having a cellulase activity of 2 WSU/ml or more, in 16 times or more diluted supernatant or broth.Type: ApplicationFiled: November 4, 2010Publication date: November 1, 2012Applicant: DSM IP ASSETS B.V.Inventors: Alrik Pieter Los, Brenda Vonk, Marco Alexander Berg Van Den, Robbertus Antonius Damveld, Cornelis Maria Jacobus Sagt, Adrianus Wilhelmus Hermanus Vollebregt, Margot Elisabeth Francoise Schooneveld-Bergmans
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Patent number: 8298804Abstract: The present invention relates to isolated structures containing degradative enzymes produced from a marine organism. The enzymes produced are based on the carbon source upon which the marine organism is growing. The enzymes are found in structures that can be isolated such that the degradative enzymes are easily harvested.Type: GrantFiled: June 26, 2007Date of Patent: October 30, 2012Assignee: University of MarylandInventors: David M. Manyak, Ronald M. Weiner, Peter S. Carlson, Ernesto J. Quintero
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Patent number: 8298795Abstract: The invention provides recombinant GH61 proteins obtained from Myceliophtora thermophila, and nucleic acids that encode such proteins. The invention also provides protein fractions isolated from M. thermophila supernatant that have GH61 protein activity. These preparations can be used to increase yield of products from reactions in which a cellulose-containing substrate undergoes saccharification by one or more cellulase enzymes, such as endoglucanase, ?-glucosidase, or cellobiohydrolase. Combinations of GH61 protein and cellulases can be used to break down cellulosic biomass into fermentable sugars in the production of ethanol.Type: GrantFiled: August 22, 2011Date of Patent: October 30, 2012Assignee: Codexis, Inc.Inventors: Jie Yang, Louis Clark, Onorato Campopiano, Kripa Rao, Lorand Szabo, Janos Torok, Dipnath Baidyaroy
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Publication number: 20120272410Abstract: The present invention relates to isolated polypeptides having cellobiohydrolase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: November 5, 2010Publication date: October 25, 2012Applicants: NOVOZYMES A/S, NOVOZYMES, INC.Inventors: Elena Vlasenko, Brett McBrayer, Mary Stringer, Kirk Schnorr
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Publication number: 20120270298Abstract: Described herein are variants of H. jecorina CBH I, a Cel7 enzyme. The present invention provides novel cellobiohydrolases that have improved thermostability and reversibility.Type: ApplicationFiled: June 14, 2012Publication date: October 25, 2012Applicants: GENENCOR INTERNATIONAL, INC., DANISCO US INC.Inventors: Anthony Day, Frits Goedegebuur, Peter Gualfetti, Colin Mitchinson, Paulien Neefe, Mats Sandgren, Andrew Shaw, Jerry Stahlberg
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Publication number: 20120270263Abstract: The invention relates to an improved enzyme complex having a plurality of enzyme activities of an expression product obtained by fermentation of the genus Trichoderma in combination with one or more enzymes of a different fungus strain.Type: ApplicationFiled: May 7, 2010Publication date: October 25, 2012Applicant: Danisco A/SInventors: Neville Marshall Fish, Lone Brønd Miller
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Publication number: 20120258520Abstract: The present invention relates to isolated polypeptides having beta-glucosidase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.Type: ApplicationFiled: May 23, 2012Publication date: October 11, 2012Applicant: NOVOZYMES. INC.Inventors: Paul Harris, Kristian Krogh
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Publication number: 20120260371Abstract: The present invention relates to isolated polypeptides having cellobiohydrolase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: October 28, 2010Publication date: October 11, 2012Applicants: NOVOZYMES A/S, NOVOZYMES, INC.Inventors: Kimberly Brown, Eric Abbate, Nikolaj Spodsberg
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Patent number: 8283150Abstract: Purified cellobiohydrolase I (glycosyl hydrolase family 7 (Cel7A)) enzymes from Penicillium funiculosum demonstrate a high level of specific performance in comparison to other Cel7 family member enzymes when formulated with purified EIcd endoglucanase from A. cellulolyticus and tested on pretreated corn stover. This result is true of the purified native enzyme, as well as recombinantly expressed enzyme, for example, that enzyme expressed in a non-native Aspergillus host. In a specific example, the specific performance of the formulation using purified recombinant Cel7A from Penicillium funiculosum expressed in A. awamori is increased by more than 200% when compared to a formulation using purified Cel7A from Trichoderma reesei.Type: GrantFiled: October 8, 2008Date of Patent: October 9, 2012Assignee: Alliance for Sustainable Energy, LLCInventors: William S. Adney, John O. Baker, Stephen R. Decker, Yat-Chen Chou, Michael E. Himmel, Shi-You Ding
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Publication number: 20120252093Abstract: Promoter regions associated with the Yarrowia lipolytica esterase/lipase (EL1) gene are disclosed and have been found to be particularly effective for the expression of heterologous genes in yeast. These promoter regions will be useful for driving high-level expression of genes involved in the production of omega-3 and omega-6 fatty acids.Type: ApplicationFiled: March 30, 2012Publication date: October 4, 2012Applicant: E I Du Pont De Nemours and CompanyInventor: Quinn Qun Zhu
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Publication number: 20120252073Abstract: A method of decomposing stubble, which is crop residue left on the ground, and a composition for treating the stubble are disclosed. The method comprises treating the stubble with the composition comprising a) polysaccharide-degrading enzyme; b) wetting agent; and, optionally, c) water and allowing the treated stubble to remain on the ground and decompose.Type: ApplicationFiled: March 28, 2012Publication date: October 4, 2012Applicant: RHODIA OPERATIONSInventors: Jean-Christophe Castaing, Gary Groves, Ashwin Rao, Thomas E. Ruch
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Publication number: 20120252064Abstract: The present invention is related to a fungal serine protease enzyme, which said enzyme has serine protease activity and comprises an amino acid sequence of Malbranchea ALKO4122 mature protease as defined in SEQ ID NO:18 or an amino acid sequence having at least 66% identity to the amino acid sequence of SEQ ID NO:18. Also disclosed is an isolated nucleic acid molecule, comprising a polynucleotide sequence which encodes a fungal serine protease enzyme, nucleic acid sequences encoding said protease, a host cell and a process of producing a polypeptide having serine protease activity. Said protease is useful as an enzyme preparation applicable in detergent compositions and for treating fibers, wool, hair, leather, or silk, for treating food or feed, or for any applications involving modification, degradation or removal of proteinaceous material.Type: ApplicationFiled: March 29, 2012Publication date: October 4, 2012Applicant: AB ENZYMES OYInventors: Leena Valtakari, Kari Juntunen, Marja Paloheimo, Pentti Ojapalo
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Patent number: 8273557Abstract: The present invention relates to cell wall degradative systems, in particular to systems containing enzymes that bind to and/or depolymerize cellulose. These systems have a number of applications. Some embodiments relate to a method of producing ethanol using the cell wall degradative systems of the present invention.Type: GrantFiled: September 12, 2006Date of Patent: September 25, 2012Assignee: University of MarylandInventors: Ronald M. Weiner, Steven Wayne Hutcheson
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Publication number: 20120237993Abstract: The present invention relates to polypeptides having cellobiohydrolase I activity and polynucleotides having a nucleotide sequence which encodes for the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid constructs as well as methods for producing and using the polypeptides.Type: ApplicationFiled: May 30, 2012Publication date: September 20, 2012Applicant: NOVOZYMES A/SInventors: Lene Lange, Wenping Wu, Dominique Aubert, Sara Landvik, Kirk Matthew Schnorr, Ib Groth Clausen
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Publication number: 20120237979Abstract: The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: September 30, 2010Publication date: September 20, 2012Applicant: NOVOZYMES A/SInventors: Junxin Duan, Kirk Matthew Schnorr, Wenping Wu
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Publication number: 20120240293Abstract: The present invention relates to isolated polypeptides having xylanase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: May 23, 2012Publication date: September 20, 2012Applicants: Novozymes, Inc., Novozymes A/SInventors: Lan Tang, Ye Liu, Junxin Duan, Hanshu Ding
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Patent number: 8268606Abstract: The present invention relates to isolated polypeptides having endoglucanase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: GrantFiled: April 20, 2010Date of Patent: September 18, 2012Assignee: Novozymes A/SInventors: Alfredo Lopez de Leon, Michael Rey
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Publication number: 20120231507Abstract: Two novel cellulases and nucleotide sequences encoding the same are disclosed. Also disclosed are compositions and methods for using the same for hydrolyzing cellulosic waste materials.Type: ApplicationFiled: March 13, 2011Publication date: September 13, 2012Applicant: Academia SinicaInventors: Yu-Chan CHAO, Chia-Jung Chang
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Publication number: 20120231504Abstract: A septum is positioned within a disposable vessel and defines a lower chamber and an upper chamber. The septum includes a plurality of apertures that provide fluid communication between the upper chamber and lower chamber. Compressed gas is introduced in the lower chamber to produce fine bubbles rising up throughout the vessel to produce a mixing and gasification needed for the growth of a biological culture and manufacture of a biological product in a nutrient medium. Adding a binding resin to the upper chamber allows harvesting, separation and purification of biological products in the reactor as a single unit operation.Type: ApplicationFiled: September 3, 2011Publication date: September 13, 2012Applicant: Therapeutic Proteins Inc.Inventor: Sarfaraz K. Niazi
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Publication number: 20120231510Abstract: The invention relates to a multi-cellulase enzyme composition for the enzymatic hydrolysis of cellulosic biomass said composition comprising a cellobiohydrolase (CBH) enzyme, an endoglucanase (EG) enzyme and a ?-glucosidase (BG) enzyme.Type: ApplicationFiled: November 18, 2010Publication date: September 13, 2012Applicant: Codexis, Inc.Inventors: Kripa Rao, Ish Dhawan, Sally Postlethwaite, Jie Yang
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Patent number: 8257955Abstract: A novel endoglucanase PPCE derived from Penicillium pinophilum, a cellulase preparation containing the endoglucanase PPCE, and a method of treating a cellulose-containing fabric utilizing the endoglucanase PPCE or the cellulase preparation, are disclosed. The endoglucanase PPCE is highly active to a fabric, and has a low optimum temperature and a strongly acidic optimum pH.Type: GrantFiled: March 12, 2008Date of Patent: September 4, 2012Assignee: Meiji Seika Pharma Co., Ltd.Inventors: Tatsuki Moriya, Akitaka Nakane, Goh Tsujiuchi, Takayoshi Fukushima
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Publication number: 20120202266Abstract: The present invention relates to isolated polypeptides having endoglucanase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.Type: ApplicationFiled: April 5, 2012Publication date: August 9, 2012Applicants: Novozymes, Inc., Novozymes A/SInventors: Paul Harris, Elena Vlasenko, Elizabeth Zaretsky, Marcus Sakari Kauppinnen
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Publication number: 20120202252Abstract: A method for recycling cellulase enzymes. Also provided is a method for producing fermentable carbohydrates, plant leaf protein, and lignin, by adding a cellulase enzyme complex expressed from and on irradiated cellulase complex-producing organisms with sufficient radiation to kill biological activity without destroying all cellulase enzyme complex activity to biomass. The fermentable carbohydrates produced by the method. Also provided are irradiated cellulase-producing organisms for use in converting biomass to fermentable sugars, plant leaf protein, and lignin. A method for producing cellulase enzymes for glucose and other sugar production and protein and lignin extraction by irradiating cellulase-producing organisms, thereby producing the cellulase enzymes is also provided. A system for producing fermentable carbohydrates, plant protein, and lignin, said system comprising irradiated cellulase-producing organisms and biomass is provided.Type: ApplicationFiled: March 15, 2012Publication date: August 9, 2012Inventor: Earnest Stuart
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Publication number: 20120202254Abstract: The present invention relates to a method for producing lactic acid from plant biomass without requiring sterilization, and specifically relates to a method for producing lactic acid comprising culturing alkaliphilic lactic acid bacteria under non-sterile condition and at a pH of 9 to 11 in a medium containing a cellulose glycosylation solution and then further culturing the bacteria at a pH of 5 to 9.Type: ApplicationFiled: October 22, 2010Publication date: August 9, 2012Applicant: NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGYInventors: Isao Yumoto, Kazuaki Yoshimune
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Publication number: 20120202262Abstract: The invention relates to a process for production of an enzyme product having a plurality of enzyme activities obtained by fermentation of an Aspergillus strain.Type: ApplicationFiled: December 21, 2009Publication date: August 9, 2012Applicant: Danisco A/SInventor: Mette Zacho Gronfeldt
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Patent number: 8236546Abstract: Disclosed are variants of Humicola grisea Cel7A (CBH1.1), H. jecorina CBH1 variant or S. thermophilium CBH1, nucleic acids encoding the same and methods for producing the same. The variant cellulases have the amino acid sequence of a glycosyl hydrolase of family 7A wherein one or more amino acid residues are substituted.Type: GrantFiled: April 21, 2011Date of Patent: August 7, 2012Assignee: Danisco US Inc.Inventors: Frits Goedegebuur, Peter Gualfetti, Colin Mitchinson, Edmund Larenas
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Publication number: 20120190099Abstract: The present invention relates to methods for degrading or converting a cellulosic material and for producing a substance from a cellulosic material.Type: ApplicationFiled: March 29, 2012Publication date: July 26, 2012Applicant: Novozymes, Inc.Inventors: Paul Harris, Michael Rey, Hanshu Ding
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Publication number: 20120190076Abstract: The invention relates to expression of a recombinant C1 ?-glucosidase. The invention also provides methods for producing a fermentable sugar from cellobiose by contacting celiobiose with a recombinant host cell comprising a polynucleotide sequence encoding C1 ?-glucosidase, operably linked to heterologous promoter, under conditions in which ?-glucosidase is expressed and secreted by the cell and the cellobiose is enzymatically converted by said ?-glucosidase to glucose. Methods of the invention may be used for conversion of a biomass substrate to a fermentable sugar, and ultimately to ethanol or other biofuel.Type: ApplicationFiled: September 30, 2010Publication date: July 26, 2012Applicant: Codexis, Inc.Inventors: Louis Clark, Dipnath Baidyaroy, Lorand Szabo
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Publication number: 20120190093Abstract: Disclosed is production of cellulase having excellent ability to decompose cellulosic resources containing xylan at low cost. A method for producing ?-glucanase and xylanase, comprising the step of culturing a microorganism classified under the genus Trichoderma by using a liquid culture medium which contains fungus body debris as an organic carbon source.Type: ApplicationFiled: August 17, 2010Publication date: July 26, 2012Applicant: ASAHI GROUP HOLDINGS, LTD.Inventor: Kazuro Fukuda
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Publication number: 20120185970Abstract: The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: March 26, 2012Publication date: July 19, 2012Applicants: Novozymes, Inc., Novozymes A/SInventors: Junxin Duan, Ye Liu, Lan Tang, Wenping Wu, Jason Quinlan, Randall Kramer