Acting On Beta-1, 4-glucosidic Bond (e.g., Cellulase, Etc. (3.2.1.4)) Patents (Class 435/209)
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Publication number: 20140115740Abstract: The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: December 13, 2013Publication date: April 24, 2014Applicants: Novozymes, Inc., Novozymes A/SInventors: Lan Tang, Ye Liu, Junxin Duan, Yu Zhang, Christian Isak Jorgensen, Randall Kramer
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Publication number: 20140113335Abstract: This invention provides novel variant cellulolytic enzymes having improved activity and/or stability. In certain embodiments the variant cellulotyic enzymes comprise a glycoside hydrolase with or comprising a substitution at one or more positions corresponding to one or more of residues F64, A226, and/or E246 in Thermobifida fusca Cel9A enzyme. In certain embodiments the glycoside hydrolase is a variant of a family 9 glycoside hydrolase. In certain embodiments the glycoside hydrolase is a variant of a theme B family 9 glycoside hydrolase.Type: ApplicationFiled: April 11, 2012Publication date: April 24, 2014Applicant: Novozymes A/SInventors: Robert Blazej, Nicholas Toriello, Charles Emrich, Richard N. Cohen, Nitzan Koppel
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Publication number: 20140113351Abstract: The present invention relates to isolated structures containing degradative enzymes produced from a marine organism. The enzymes produced are based on the carbon source upon which the marine organism is growing. The enzymes are found in structures that can be isolated such that the degradative enzymes are easily harvested.Type: ApplicationFiled: October 18, 2012Publication date: April 24, 2014Inventors: David M. Manyak, Ronald M. Weiner, Peter S. Carlson, Ernesto J. Quintero
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Patent number: 8703464Abstract: The present invention relates to enzyme compositions comprising a polypeptide having cellobiohydrolase II activity, a polypeptide having xylanase activity, and one or more cellulolytic proteins and their use in the degradation or conversion of cellulosic material.Type: GrantFiled: August 26, 2013Date of Patent: April 22, 2014Assignee: Novozymes, Inc.Inventors: Kimberly Brown, Eric Abbate
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Publication number: 20140106408Abstract: The present invention relates to compositions that can be used in hydrolyzing biomass such as compositions comprising a polypeptide having glycosyl hydrolase (GH) family 61/endoglucanase activity and/or a ?-glucosidase polypeptide, methods for hydrolyzing biomass material, and methods for using such compositions.Type: ApplicationFiled: March 16, 2012Publication date: April 17, 2014Applicant: DANISCO US INC.Inventors: Colin Mitchinson, Steven S. Kim, Meredith K. Fujdala, Megan Y. Hsi, Keith D. Wing, William D. Hitz
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Publication number: 20140106434Abstract: The present disclosure relates to methods of increasing the production of an apoplast-targeted recombinant protein in a plant tissue and to methods of increasing the purity of an apoplast-targeted recombinant protein recovered from a plant tissue.Type: ApplicationFiled: October 11, 2013Publication date: April 17, 2014Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIAInventors: Karen A. MCDONALD, Nathaniel J. KINGSBURY
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Publication number: 20140099678Abstract: Novel fungal endoglucanases Cel5 and Cel12 are disclosed. The endoglucanases are conveniently produced by recombinant technology, and means for their production are described. The endoglucanases are used for treating cellulosic material, especially in textile industry, e.g. in biofinishing or biostoning. They may also be used in detergents, in animal feed and/or in pulp and paper industry, or in hydrolysis of lignocellulosic material for, e.g. bioethanol production.Type: ApplicationFiled: October 18, 2013Publication date: April 10, 2014Applicant: AB Enzymes OyInventors: Terhi PURANEN, Leena VALTAKARI, Marika ALAPURANEN, George SZAKACS, Pentti OJAPALO, Jari VEHMAANPERA
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Publication number: 20140099696Abstract: Microspheres are produced by contacting a solution of a macromolecule or small molecule in a solvent with an antisolvent and a counterion, and chilling the solution. The microspheres are useful for preparing pharmaceuticals, nutraceuticals, cosmetic products and the like of defined dimensions.Type: ApplicationFiled: April 30, 2013Publication date: April 10, 2014Applicant: Ansun Biopharma, Inc.Inventors: Michael P. Malakhov, Fang Fang
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Publication number: 20140099677Abstract: This present disclosure relates to methods for improved production of proteins from a cell culture, particularly to culture components and conditions that can preferentially increase the expression of proteins produced from genes under the control of xylanase gene promoter sequences. The improved methods can be used for the production of enzyme compositions with enhanced xylanase and hem icellulolytic activity.Type: ApplicationFiled: August 15, 2013Publication date: April 10, 2014Applicant: Danisco US Inc.Inventors: Aaron KELLEY, Chuanbin LIU, Colin MITCHINSON
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Publication number: 20140101793Abstract: The present invention relates to isolated polypeptides having beta-glucosidase activity, beta-xylosidase activity, or beta-glucosidase and beta-xylosidase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: December 12, 2013Publication date: April 10, 2014Applicants: Novoyzmes A/S, Novozymes, Inc.Inventor: Marc Dominique Morant
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Publication number: 20140093940Abstract: The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.Type: ApplicationFiled: October 12, 2012Publication date: April 3, 2014Applicant: Novozymes, Inc.Inventors: Kimberly Brown, Paul Harris, Elizabeth Zaretsky, Edward Re, Elena Vlasenko, Keith McFarland, Alfredo Lopez de Leon
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Publication number: 20140093919Abstract: The present invention relates to isolated polypeptides having endoglucanase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: July 31, 2012Publication date: April 3, 2014Applicant: Novozymes Inc.Inventor: Nikolaj Spodsberg
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Publication number: 20140096287Abstract: The present invention relates to isolated polypeptides having endoglucanase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: November 14, 2013Publication date: April 3, 2014Inventors: Kristian Krogh, Paul Harris
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Patent number: 8685690Abstract: The invention relates to recombinant expression of a variant form of a fungal C1 strain ?-glucosidase. The invention also relates to the generation of fermentable sugars from biomass and the production of biofuels by fermentation of the sugars using genetically modified organisms expressing the ?-glucosidase variant. The invention provides methods for producing a fermentable sugar, such as glucose, from cellobiose by contacting cellobiose with a recombinant ?-glucosidase variant protein, such as a variant protein secreted by a recombinant host cell into culture medium. Methods of the invention may be used for conversion of a biomass substrate to a fermentable sugar, and ultimately to ethanol or other biofuel.Type: GrantFiled: March 9, 2012Date of Patent: April 1, 2014Assignee: Codexis, Inc.Inventors: Jie Yang, Xiyun Zhang, Attila Andor
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Publication number: 20140087405Abstract: The object is to provide an ?-glucosidase in which a transglycosylation activity predominates, and use thereof, and the like. A modified ?-glucosidase consisting of an amino acid sequence in which one or two or more of the amino acid(s) is selected from a group of specific amino acids.Type: ApplicationFiled: March 5, 2012Publication date: March 27, 2014Applicant: AMANO ENZYME INC.Inventor: Satoru Ishihara
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Patent number: 8679791Abstract: The present invention relates to a mashing and filtration step in a brewing process and to a composition useful in the mashing and filtration step of a brewing process.Type: GrantFiled: June 29, 2009Date of Patent: March 25, 2014Assignee: Novozymes A/SInventors: Rikke Monica Festersen, Anders Viksoe Nielsen, Christel Thea Joergensen, Lars Lehmann Hylling Christensen
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Patent number: 8679824Abstract: The present disclosure relates to fermentation broth formulations containing organic acids and/or organic acid salts, and methods of making and using such formulations.Type: GrantFiled: February 19, 2010Date of Patent: March 25, 2014Assignee: Danisco US Inc.Inventors: Tom Tao Huang, Aaron Kelley, John McLaughlin
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Patent number: 8679818Abstract: The present invention provides a novel ?-glucosidase nucleic acid sequence, designated bgl7, and the corresponding BGL7 amino acid sequence. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding BGL7, recombinant BGL7 proteins and methods for producing the same.Type: GrantFiled: December 17, 2012Date of Patent: March 25, 2014Assignee: Danisco US Inc.Inventors: Nigel Dunn-Coleman, Michael Ward
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Patent number: 8679794Abstract: Method for extracting oils, proteins and fermentable sugars from vegetable material in an aqueous medium, includes: a) adding water to the vegetable material; b) adding an enzyme mixture containing at least one cellulase, at least one hemicellulase, and at least one pectinase, the ratio between the pectinase activity and the cellulase activity being at least 0.14, and the ratio between the pectinase activity and the hemicellulase activity being at least 7.10?3, the pectinase activity being less than 120 ?mol/min/mL; c) incubating the vegetable material and the enzyme mixture with stirring to release oils, proteins and fermentable sugars in the reaction medium; d) separating the reaction medium to obtain free oil, an aqueous phase containing proteins and fermentable sugars, and a solid phase; e) optionally separating and recycling an emulsion of free oil or aqueous phase, to the medium; f) separating the proteins and fermentable sugars from the aqueous phase.Type: GrantFiled: October 14, 2010Date of Patent: March 25, 2014Assignee: Universite de LorraineInventors: Lionel Muniglia, Michel Girardin, Bernadette Piffaut, Guillaume Ricochon
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Patent number: 8679817Abstract: Described herein are variants of H. jecorina CBH I, a Cel7 enzyme. The present invention provides novel cellobiohydrolases that have improved thermostability and reversibility.Type: GrantFiled: June 14, 2012Date of Patent: March 25, 2014Assignee: Danisco US Inc.Inventors: Anthony Day, Frits Goedegebuur, Peter Gualfetti, Colin Mitchinson, Paulien Neefe-Kruithof, Mats Sandgren, Andrew Shaw, Jerry Stahlberg
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Patent number: 8679816Abstract: The present disclosure relates to cellulase variants. In particular the present disclosure relates to cellulase variants having improved expression, activity and/or stability. Also described are nucleic acids encoding the cellulase variants, compositions comprising the cellulase variants, and methods of use thereof.Type: GrantFiled: June 3, 2010Date of Patent: March 25, 2014Assignee: Danisco US Inc.Inventors: Wolfgang Aehle, Richard R. Bott, Benjamin Bower, Jonathan Caspi, David A. Estell, Frits Goedegebuur, Ronaldus W. J. Hommes, Thijs Kaper, Bradley Kelemen, Slavko Kralj, Johan Van Lieshout, Igor Nikolaev, Sander Van Stigt Thans, Louise Wallace, Gudrun Vogtentanz, Mats Sandgren
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Publication number: 20140080193Abstract: The present invention relates to methods for degrading or converting a cellulosic material and for producing a substance from a cellulosic material.Type: ApplicationFiled: March 30, 2012Publication date: March 20, 2014Inventors: Kirk Schnorr, Randall Kramer
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Publication number: 20140080182Abstract: The present invention relates to cellobiohydrolase variants and cellulose binding domain variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.Type: ApplicationFiled: March 30, 2012Publication date: March 20, 2014Applicant: Novozymes, Inc.Inventors: Aubrey Jones, Paul Harris, Tia Heu, Doreen Bohan
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Patent number: 8673611Abstract: Disclosed are variants of Humicola grisea Cel7A (CBH1.1), H. jecorina CBH1 variant or S. thermophilium CBH1, nucleic acids encoding the same and methods for producing the same. The variant cellulases have the amino acid sequence of a glycosyl hydrolase of family 7A wherein one or more amino acid residues are substituted.Type: GrantFiled: July 5, 2012Date of Patent: March 18, 2014Assignee: Danisco US Inc.Inventors: Frits Goedegebuur, Peter Gualfetti, Colin Mitchinson, Edmund Larenas
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Patent number: 8674178Abstract: Described herein are methods useful for producing proteins, such as enzymes, by agrofiltration. The methods involve producing an Agrobacterium with a Ti plasmid encoding a cellulase, infecting plant cells with the Agrobacterium, allowing expression of the cellulase, and recovering the cellulase from the plant cells. In one embodiment, the protein produced is an endoglucanase.Type: GrantFiled: August 19, 2009Date of Patent: March 18, 2014Assignee: The Regents of the University of CaliforniaInventors: Karen A. McDonald, Benjamin E. Lindenmuth, Abhaya M. Dandekar, Bryce W. Falk
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Publication number: 20140073012Abstract: The present disclosure provides compositions and methods related to the degradation of cellulose and cellulose-containing materials. CDH-heme domain polypeptides and GH61 polypeptides and related polynucleotides and compositions are provided herein.Type: ApplicationFiled: April 4, 2012Publication date: March 13, 2014Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIAInventors: Michael A. Marletta, James H. Doudna Cate, William T. Beeson, IV, Christopher M. Phillips
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Publication number: 20140073017Abstract: The present invention relates to compositions that can be used in hydrolyzing biomass such as compositions comprising a polypeptide having ?-glucosidase activity, methods for hydrolyzing biomass material, and methods for improving the stability and saccharification efficacy of a composition comprising such ?-glucosidase polypeptides and/or activity.Type: ApplicationFiled: March 16, 2012Publication date: March 13, 2014Applicant: DANISCO US INC.Inventors: Thijs Kaper, Igor Nikolaev, Suzanne E. Lantz, Meredith K. Fujdala, Megan Y. Hsi
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Publication number: 20140065678Abstract: The invention relates to recombinant expression of a variant form of a fungal C1 strain ?-glucosidase. The invention also relates to the generation of fermentable sugars from biomass and the production of biofuels by fermentation of the sugars using genetically modified organisms expressing the ?-glucosidase variant. The invention provides methods for producing a fermentable sugar, such as glucose, from cellobiose by contacting cellobiose with a recombinant ?-glucosidase variant protein, such as a variant protein secreted by a recombinant host cell into culture medium. Methods of the invention may be used for conversion of a biomass substrate to a fermentable sugar, and ultimately to ethanol or other biofuel.Type: ApplicationFiled: November 8, 2013Publication date: March 6, 2014Applicant: Codexis, Inc.Inventors: Jie Yang, Xiyun Zhang, Attila Andor
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Publication number: 20140065677Abstract: The present invention relates to isolated polypeptides having cellulolytic activity or hemicellulolytic activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: January 26, 2012Publication date: March 6, 2014Applicant: Novozymes A/SInventors: Lene Lange, Peter K. Busk
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Publication number: 20140065671Abstract: The present invention relates to isolated polypeptides having cellobiohydrolase activity, catalytic domains, and cellulose binding domains and polynucleotides encoding the polypeptides, catalytic domains, and cellulose binding domains. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains, or cellulose binding domains.Type: ApplicationFiled: January 26, 2012Publication date: March 6, 2014Applicants: Novozymes, Inc., Novozymes A/SInventors: Mary Ann Stringer, Brett McBrayer
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Publication number: 20140065693Abstract: The present invention relates to the production of sugar hydrolysates from cellulosic material. The method may be used, for example for producing fermentable sugars for the production of bioethanol from lignocellulosic material. Cellulolytic enzymes and their production by recombinant technology are described, as well as uses of the enzymes and enzyme preparations.Type: ApplicationFiled: October 9, 2013Publication date: March 6, 2014Applicant: ROAL OYInventors: Terhi Puranen, Sauli Toikka, Kim Langfelder, Jari Vehmaanpera
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Publication number: 20140058790Abstract: Methods and compositions described herein relate to processes for the production of deuterated peptides, and the deuterated peptides produced accordingly. Deuterated peptides produced according to methods and compositions described herein may be produced more efficiently than such peptides produced according to prior art processes. The production process of according to methods and compositions described herein may lead to advantages in yield, purity, and/or price for deuterated peptides. Methods of marketing deuterated peptides are also disclosed.Type: ApplicationFiled: November 30, 2011Publication date: February 27, 2014Applicant: AmideBio LLCInventors: Michael H.B. Stowell, Mikhail Plam
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Patent number: 8658406Abstract: The present invention provides a novel endoglucanase nucleic acid sequence, designated egl7, and the corresponding EGVII amino acid sequence. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding EGVII, recombinant EGVII proteins and methods for producing the same.Type: GrantFiled: June 17, 2013Date of Patent: February 25, 2014Assignee: Danisco US Inc.Inventors: Nigel Dunn-Coleman, Frits Goedegebuur, Michael Ward, Jian Yao
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Publication number: 20140051119Abstract: The present invention provides fungal xylanase and/or beta-xylosidase enzymes suitable for use in saccharification reactions. The present application further provides genetically modified fungal organisms that produce xylanase and/or beta-xylosidases, as well as enzyme mixtures exhibiting enhanced hydrolysis of cellulosic material to fermentable sugars, enzyme mixtures produced by the genetically modified fungal organisms, and methods for producing fermentable sugars from cellulose using such enzyme mixtures.Type: ApplicationFiled: June 10, 2013Publication date: February 20, 2014Applicant: Codexis, Inc.Inventors: Ryan Fong, Xiyun Zhang, Chirs Noriega, Nicholas Agard, Anupam Gohel, Derek Smith
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Publication number: 20140051147Abstract: A method for suppressing a reduction in an endoglucanase activity in the presence of a surfactant, characterized by modifying a protein having the endoglucanase activity in which the N-terminus is an amino acid other than pyroglutamic acid, to a protein having the N-terminus of pyroglutamic acid, is disclosed. Further, a modified protein having an endoglucanase activity wherein the N-terminal amino acid is converted into pyroglutamic acid by an amino acid modification, a polynucleotide encoding the protein, an expression vector comprising the polynucleotide, a host cell transformed with the expression vector, and a process for producing the protein by cultivating the host cell, are disclosed.Type: ApplicationFiled: September 19, 2013Publication date: February 20, 2014Applicant: MEIJI SEIKA PHARMA CO., LTD.Inventors: Manabu WATANABE, Koji YANAI, Yumiko TSUYUKI
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Publication number: 20140051130Abstract: The present invention relates to enzyme compositions for high temperature saccharification of cellulosic material and to uses thereof.Type: ApplicationFiled: October 11, 2013Publication date: February 20, 2014Inventors: Brett McBrayer, Tarana Shaghasi, Elena Vlasenko
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Publication number: 20140051142Abstract: The present invention relates to polypeptides having cellobiohydrolase I activity and polynucleotides having a nucleotide sequence which encodes for the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid constructs as well as methods for producing and using the polypeptides.Type: ApplicationFiled: October 28, 2013Publication date: February 20, 2014Applicant: Novozymes A/SInventors: Lene Lange, Wenping Wu, Dominique Aubert, Sara Landvik, Kirk Matthew Schnorr, Ib Groth Clausen
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Publication number: 20140051143Abstract: The present invention relates to polypeptides having cellobiohydrolase I activity and polynucleotides having a nucleotide sequence which encodes for the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid constructs as well as methods for producing and using the polypeptides.Type: ApplicationFiled: October 28, 2013Publication date: February 20, 2014Applicant: NOVOZYMES A/SInventors: Lene Lange, Wenping Wu, Dominique Aubert, Sara Landvik, Kirk Matthew Schnorr, Ib Groth Clausen
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Publication number: 20140051128Abstract: The present invention relates to production of fermentable sugars from lignocellulosic material by enzymatic conversion. The fermentable sugars are useful e.g. in the production of bioethanol. Novel polypeptides having endoglucanase activity, polynucleotides encoding them and vectors and host cells containing the polynucleotides are disclosed. A method for treating cellulosic material with the novel endoglucanase as well as uses of the enzymes and enzyme preparations and a method of preparing them are described.Type: ApplicationFiled: May 24, 2013Publication date: February 20, 2014Applicant: ROAL OYInventors: Taija LEINONEN, Alexandra KOMANDER, Kim LANGFELDER, Jari VEHMAANPERÄ, Terhi PURANEN
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Publication number: 20140045243Abstract: The present disclosure provides mutant cells for the secretion of proteins and for the degradation of lignocellulosic biomass. Methods for the use of these cells are also provided. Specifically, the utility of combined genetic deletions of ?-glucosidases and the catabolite repressor gene creA/cre-1 for protein secretion in fungal and yeast cells is disclosed.Type: ApplicationFiled: March 15, 2012Publication date: February 13, 2014Applicant: The Regents of the Uniersity of CaliforniaInventors: Elizabeth A. Znameroski, James H. Doudna Cate, N. Louise Glass
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Publication number: 20140045225Abstract: The present invention discloses novel polypeptides and enzyme preparations containing them, which enhance the efficiency of the cellulosic degradation even at elevated temperatures. The polypeptides are produced by recombinant technology, and means for their production are described. The novel polypeptides are useful in processing biomass, and in biofuel, starch, textile, detergent, pulp and paper, food, feed or beverage industries. They may also be used e.g. in cleaning the interior of a dishwashing machine or for biofinishing or biostoning. The novel polypeptides are also useful in animal feed.Type: ApplicationFiled: June 6, 2013Publication date: February 13, 2014Applicant: Roal OyInventors: Susanna MAKINEN, Kari JUNTUNEN, Alexandra KOMANDER, Kim LANGFELDER, Jari VEHMAANPERA, Terhi PURANEN
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Publication number: 20140038262Abstract: The present invention relates to the novel Paenibacillus sp. strain, and the novel protein isolated from the same. More particularly, the present invention relates to the novel Paenibacillus sp. strain producing xylanase, and the novel xylanase having high activity at high temperature and in a wide range of pH, and a production method of the same. The Paenibacillus sp. HPL-3 strain (KCTC11987BP) and the xylanase of the present invention demonstrates high activity at high temperature or in a wide range of pH to decompose xylan, the major component of various lignocellulosic biomass, so that they can be effectively used for the production or development of bio-fuel, alternative material, performance chemical, bio-polymer, food and feeds, etc.Type: ApplicationFiled: August 12, 2011Publication date: February 6, 2014Applicant: KOREA RESEARCH INSTITUTE OF CHEMICAL TECHNOLOGYInventors: In Taek Hwang, No Joong Park, He Kyoung Lim
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Publication number: 20140030770Abstract: The present invention relates to isolated polypeptides having cellobiohydrolase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: January 26, 2012Publication date: January 30, 2014Applicant: NOVOZYMES INC.Inventor: Nikolaj Spodsberg
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Publication number: 20140030769Abstract: Disclosed herein are combinations of free fungal enzymes and cellulosomes useful for the hydrolysis of cellulose and the conversion of biomass. Methods of degrading cellulose and biomass using the combinations are also disclosed.Type: ApplicationFiled: July 29, 2013Publication date: January 30, 2014Applicant: Alliance for Sustainable Energy, LLCInventors: Michael RESCH, John O. BAKER, Xu QI, William S. ADNEY, Steven R. DECKER, Michael E. HIMMEL, Bryon DONOHOE
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Publication number: 20140033373Abstract: The invention relates to a polypeptide comprising the amino acid sequence set out in SEQ ID NO: 2 or an amino acid sequence encoded by the nucleotide sequence of SEQ ID NO: 1, or a variant polypeptide or variant polynucleotide thereof, wherein the variant polypeptide has at least 73% sequence identity with the sequence set out in SEQ ID NO: 2 or the variant polynucleotide encodes a polypeptide that has at least 73% sequence identity with the sequence set out in SEQ ID NO: 2. The invention features the full length coding sequence of the novel gene as well as the amino acid sequence of the full-length functional polypeptide and functional equivalents of the gene or the amino acid sequence. The invention also relates to methods for using the polypeptide in industrial processes. Also included in the invention are cells transformed with a polynucleotide according to the invention suitable for producing these proteins.Type: ApplicationFiled: June 23, 2011Publication date: January 30, 2014Applicant: DSM IP ASSETS B.V.Inventors: Margot Elisabeth Francoise Schooneveld-Bergmans, Wilbert Herman Marie Heijne, Monica Diana Vlasie, Robbertus Antonius Damveld
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Patent number: 8637284Abstract: Biomass (e.g., plant biomass, animal biomass, microbial, and municipal waste biomass) is processed to produce useful products, such as food products and amino acids.Type: GrantFiled: December 6, 2011Date of Patent: January 28, 2014Assignee: Xyleco, Inc.Inventor: Marshall Medoff
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Patent number: 8637294Abstract: Described herein are variants of H. jecorina CBH I, a Cel7 enzyme. The present invention provides novel cellobiohydrolases that have improved thermostability and reversibility.Type: GrantFiled: March 22, 2007Date of Patent: January 28, 2014Assignee: Danisco US Inc.Inventors: Anthony Day, Frits Goedegebuur, Peter Gualfetti, Colin Mitchinson, Paulien Neefe, Mats Sandgren, Andrew Shaw, Jerry Stahlberg
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Publication number: 20140017733Abstract: The present invention provides fungal xylanase and/or xylosidase enzymes suitable for use in saccharification reactions. The present invention provides xylanase and xylosidase enzymes suitable for use in saccharification reactions. The present application further provides genetically modified fungal organisms that produce xylanase(s) and/or xylosidase(s), as well as enzyme mixtures exhibiting enhanced hydrolysis of cellulosic material to fermentable sugars, enzyme mixtures produced by the genetically modified fungal organisms, and methods for producing fermentable sugars from cellulose using such enzyme mixtures. In some embodiments, the xylanase and xylosidase enzyme(s) are M. thermophila enzymes.Type: ApplicationFiled: June 10, 2013Publication date: January 16, 2014Inventors: Nicholas John Agard, David Elgart, Jie Yang, Goutami Banerjee, Jeanne Bonomo Benoit, Dipnath Baidyaroy
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Publication number: 20140017737Abstract: The present invention relates to variants of a parent cellobiohydrolase II. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.Type: ApplicationFiled: September 23, 2013Publication date: January 16, 2014Inventor: Mark Wogulis
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Publication number: 20140017735Abstract: Disclosed herein are enzymes and combinations of the enzymes useful for the hydrolysis of cellulose and the conversion of biomass. Methods of degrading cellulose and biomass using enzymes and cocktails of enzymes are also disclosed.Type: ApplicationFiled: July 15, 2013Publication date: January 16, 2014Inventors: Roman BRUNECKY, Michael E. HIMMEL