Proteinase Patents (Class 435/219)
Derived from bacteria (Class 435/220)
Derived from fungi (Class 435/223)
Derived from animal tissue (e.g., rennin, etc.) (Class 435/226)
  • Thermostable proteolytic enzymes and uses thereof in peptide protein synthesis
    Patent number: 6573065
    Abstract: The subject invention pertains to new thermostable enzymes and the use of these enzymes both in proteolysis as well as protein and polypeptide synthesis. The subject invention further concerns polynucleotide sequences which encode the enzymes of the subject invention.
    Type: Grant
    Filed: September 23, 1999
    Date of Patent: June 3, 2003
    Inventor: David Michael Young
  • Calpaines, production and use thereof
    Patent number: 6569665
    Abstract: Novel calpains and their preparation, and methods for screening for novel calpain inhibitors and their use are described.
    Type: Grant
    Filed: May 19, 1999
    Date of Patent: May 27, 2003
    Assignee: BASF Aktiengesellschaft
    Inventors: Thomas Boehm, Neil T. Dear
  • Assay for modulators of metallo-enzyme activity
    Patent number: 6569636
    Abstract: An assay is disclosed for determining whether a test compound modulates the activity of an enzyme having a metallated active site. The assay method employs a comparison of the binding ability of the metallated and unmetallated forms of the enzyme to the test compound.
    Type: Grant
    Filed: May 27, 1999
    Date of Patent: May 27, 2003
    Assignee: Hawaii Biotechnology Group, Inc.
    Inventors: Paul G. Grothaus, Dana E. Davis, Sean O'Malley
  • Nucleic acids and polypeptides
    Patent number: 6569662
    Abstract: The present invention provides novel nucleic acids, novel polypeptide sequences encoded by these nucleic acids and uses thereof.
    Type: Grant
    Filed: July 19, 2000
    Date of Patent: May 27, 2003
    Assignee: Hyseq, Inc.
    Inventors: Y. Tom Tang, Ping Zhou, Radoje T. Drmanac
  • Biochemical and genetic characterization of propionicin T1
    Publication number: 20030096365
    Abstract: Propionicin T1 has been isolated from Propionibacterium thoenii and both the genetic operon and the products encoded thereby have been characterized. The operon contains two genes: a bacteriocin and an ABC transporter which may be an immunity factor which increases resistance to the bacteriocin. Processes for using and making these products are also provided.
    Type: Application
    Filed: September 24, 2002
    Publication date: May 22, 2003
    Inventors: Therese Faye, Helge Holo, Thor Langsrud, Ingolf Nes
  • Caspase inhibitors for the treatment and prevention of chemotherapy and radiation therapy induced cell death
    Patent number: 6566338
    Abstract: The use of caspase inhibitors for treating, ameliorating, and preventing non-cancer cell death during chemotherapy and radiation therapy and for treating and ameliorating the side effects of chemotherapy and radiation therapy of cancer is disclosed.
    Type: Grant
    Filed: October 11, 2000
    Date of Patent: May 20, 2003
    Assignee: Cytovia, Inc.
    Inventors: Eckard Weber, Gordon B. Mills, Douglas R. Green
  • Nucleic acid encoding RNA ligase of bacteriophage RM 378
    Publication number: 20030092128
    Abstract: A novel bacteriophage RM 378 of Rhodothermus marinus, the nucleic acids of its genome, nucleic acids comprising nucleotide sequences of open reading frames (ORFs) of its genome, and polypeptides encoded by the nucleic acids, are described.
    Type: Application
    Filed: October 11, 2002
    Publication date: May 15, 2003
    Applicant: Prokaria ltd.
    Inventors: Sigridur Hjorleifsdottir, Gudmundur O. Hreggvidsson, Olafur H. Fridjonsson, Arnthor Aevarsson, Jakob K. Kristjansson
  • Recombinant C-proteinase and processes, methods and uses thereof
    Patent number: 6562613
    Abstract: The present invention is directed to the isolation and identification of the nucleic acid sequence encoding C-proteinase, the recognition of such protein's activity and applications, and tools, processes, and methods of use thereof.
    Type: Grant
    Filed: May 7, 2001
    Date of Patent: May 13, 2003
    Assignee: Thomas Jefferson University
    Inventors: Darwin J. Prockop, Yoshio Hojima, Shi-Wu Li, Aleksander Sieron
  • Modified polypeptides
    Publication number: 20030087785
    Abstract: The present invention relates to polypeptides with reduced immune response including reduced allergenicity having one or more amino acid residues being substituted with other amino acid residues and/or having coupled one or more polymeric molecules in the vicinity of the polypeptides metal binding site, a method for preparing modified polypeptides of the invention, the use of the polypeptide for reducing the immunogenicity and allergenicity and compositions comprising the polypeptide.
    Type: Application
    Filed: August 1, 2002
    Publication date: May 8, 2003
    Applicant: Novozymes A/S
    Inventors: Arne Agerlin Olsen, Claus von der Osten, Kim Vilbour Andersen, Steffen Ernst, Erwin Ludo Roggen
  • Genes enhancing disease resistance in plants
    Publication number: 20030084477
    Abstract: The present invention relates to methods and materials for the protection of plants against pathogens through plant genetic engineering; and more particularly to genes which enhance disease resistance in plants by encoding proteins that physically interact with R gene products involved in activation of plant defense mechanisms. The invention further relates to three nucleotide sequences which have been cloned, isolated and sequenced, three amino acid sequences encoded thereby and a transgenic plant and methods for making the same, the genome of the plant having incorporated therein a foreign nucleotide sequence selected in accordance with the invention which functions to enhance the plant's ability to resist pathogens.
    Type: Application
    Filed: April 15, 2002
    Publication date: May 1, 2003
    Inventors: Gregory B. Martin, Jian-Min Zhou
  • Immunization of dairy cattle with GapC protein against Streptococcus infection
    Publication number: 20030082781
    Abstract: The GapC plasmin binding protein genes of Streptococcus dysgalactiae (S. dysgalactiae), Streptococcus agalactiae (S. agalactiae), Streptococcus uberis (S. uberis), Streptococcus parauberis (S. parauberis), and Streptococcus iniae (S. iniae) are described, as well as the recombinant production of the GapC proteins therefrom. Also described is the use of the GapC proteins from those species in vaccine compositions to prevent or treat bacterial infections in general, and mastitis in particular.
    Type: Application
    Filed: June 11, 2001
    Publication date: May 1, 2003
    Inventors: Alexandra J. Bolton, Jose Perez-Casal, Michael Fontaine, Andrew A. Potter
  • Polypeptide inducing antibodies neutralizing HIV
    Publication number: 20030082521
    Abstract: The present invention thus provides a polypeptide capable of forming a structure corresponding to or mimicking the intermediate of gp41 as well as its use in a vaccine for treating or preventing HIV infections.
    Type: Application
    Filed: January 7, 2002
    Publication date: May 1, 2003
    Inventors: Robert Brasseur, Benoit Charloteaux, Michel Chevalier, Raphaelle El Habib, Tino Krell, Regis Sodoyer
  • Nucleic acid fragments and proteins affecting storage organelle formation and methods of use
    Publication number: 20030084475
    Abstract: This invention relates to an isolated nucleic acid fragment encoding an SSE1 protein. The invention also relates to the construction of a chimeric gene encoding all or a portion of the SSE1 protein, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the SSE1 protein in a transformed host cell. The present invention also relates to methods using the SSE1 protein in modulating formation of storage organelles and storage compounds in seeds, and in discovering compounds with potential herbicidal activity.
    Type: Application
    Filed: October 9, 2002
    Publication date: May 1, 2003
    Inventors: Edgar B. Cahoon, Sean J. Coughlan, Timothy George Helentjaris, Rudolf Jung, Chun Ping Li, Scott E. Nichols, Kevin G. Ripp, Peizhong Zheng
  • Fusion proteins, DNA molecules, vectors, and host cells useful for measuring protease activity
    Publication number: 20030083467
    Abstract: The present invention relates to novel fusion proteins, DNA molecules encoding the same, vectors comprising the DNA molecules, and host cells containing the vectors for use in measuring protease activity using a novel transcriptional assay. This invention also relates to a method for determining the inhibitory activity of a compound against a protease and to a method for comparing the activity of two proteases which recognize the same cleavage site. Kits for assaying protease activity comprising DNA molecules encoding the fusion protein substrates of this invention are also contemplated.
    Type: Application
    Filed: November 20, 2002
    Publication date: May 1, 2003
    Inventors: Ursula Germann, Thomas Hoock, Ann Kwong
  • Novel secretion factors for gram-positive micoorganisms, genes encoding them and methods of using it
    Publication number: 20030077729
    Abstract: Heterologous protein secretion from Gram-positive bacteria, in particular from Bacilli has, with few exceptions, met with little success. Incompatibility of the heterologous proteins with the protein secretion machinery of the host is the main cause of this effect. This limiting factor for the production of heterologous proteins in commercially significant concentrations from Bacillus subtilis is removed by overexpressing the Bacillus subtilis protein FtsY or FtsY protein in combination with overexpression of other members of the bacterial signal recognition particle. Said gene(s) is(are) overexpressed in Bacillus host cells expressing a heterologous protein which then shows an increased amount of the heterologous protein secreted in the surrounding medium.
    Type: Application
    Filed: May 17, 2002
    Publication date: April 24, 2003
    Inventors: Wilhelmus J. Quax, Richard Kerkman, Cornelis P. Broekhuizen
  • D-enzyme compositions and methods of their use
    Patent number: 6548279
    Abstract: D-enzyme compositions are described comprising an amino acid residue sequence that defines an polypeptide able to catalyze an enzymatic reaction. The D-enzyme has an amino acid residue sequence consisting of D-amino acids and glycine.
    Type: Grant
    Filed: December 2, 1994
    Date of Patent: April 15, 2003
    Assignee: The Scripps Research Institute
    Inventors: Stephen Brian Henry Kent, Saskia Charlotte Florence Milton, Raymond Cecil deLisle Milton
  • Compositions and methods for the diagnosis and threatment of herpes simplex virus infection
    Publication number: 20030068327
    Abstract: Compounds and methods for the diagnosis and treatment of HSV infection are provided. The compounds comprise polypeptides that contain at least one antigenic portion of an HSV polypeptide and DNA sequences encoding such polypeptides. Pharmaceutical compositions and vaccines comprising such polypeptides or DNA sequences are also provided, together with antibodies directed against such polypeptides. Diagnostic kits are also provided comprising such polypeptides and/or DNA sequences and a suitable detection reagent for the detection of HSV infection in patients and in biological samples.
    Type: Application
    Filed: April 11, 2002
    Publication date: April 10, 2003
    Applicant: Corixa Corporation
    Inventors: Nancy Ann Hosken, Patrick McGowan, Paul R. Sleath, Sally P. Mossman, Lawrence S. Evans, Ryan M. Swanson, Patricia D. McNeill
  • Cathespin O2 protease
    Patent number: 6544767
    Abstract: The invention relates to cathepsin O2 proteins, nucleic acids, and antibodies.
    Type: Grant
    Filed: October 2, 1995
    Date of Patent: April 8, 2003
    Assignee: Axys Pharmaceuticals, Inc.
    Inventors: Dieter Brömme, Kathleen Okamoto
  • Efficient methods for the preparation of rhinovirus protease inhibitors, key intermediates and a continuous membrane reactor useful for preparing the same
    Publication number: 20030064429
    Abstract: Efficient synthetic routes for the preparation of rhinovirus protease inhibitors of formula I, key intermediates useful in those synthetic routes, as well as a continuous membrane reactor useful for those synthetic routes. These compounds of formula I, as well as pharmaceutical compositions that contain these compounds, are suitable for treating patients or hosts infected with one or more picornaviruses.
    Type: Application
    Filed: July 25, 2002
    Publication date: April 3, 2003
    Applicant: Agouron Pharmaceuticals, Inc.
    Inventors: Junhua Tao, Srinivasan Babu, Raymond Dagnino, Qingping Tian, Travis Paul Remarchuk, Kevin Scott McGee, Naresh K. Nayyar, Terence Jarold Moran
  • Hepatitis C virus protease
    Publication number: 20030064499
    Abstract: The protease necessary for polyprotein processing in Hepatitis C virus is identified, cloned, and expressed. Proteases, truncated protease, and altered proteases are disclosed which are useful for cleavage of specific polypeptides, and for assay and design of antiviral agents specific for HCV.
    Type: Application
    Filed: June 18, 2001
    Publication date: April 3, 2003
    Inventors: Michael Houghton, Qui-Lim Choo, George Kuo
  • Novel plant promoter sequences and methods of use for same
    Publication number: 20030066108
    Abstract: The invention discloses novel promoter sequences capable of expressing genes in plant cells. The promoters include engineered versions of the maize ubiquitin promoter to increase expression levels beyond those observed with the native ubiquitin promoter and alter the tissue preference. Expression constructs, vectors, transgenic plants and methods are also disclosed.
    Type: Application
    Filed: February 28, 2002
    Publication date: April 3, 2003
    Applicant: Prodigene
    Inventors: Joseph M. Jilka, Elizabeth E. Hood, John A. Howard
  • Metabolic genes and related methods and compositions
    Publication number: 20030064931
    Abstract: Certain aspects of the invention provide nucleic acid constructs that can be used to cause a cell to be dependent on a particular enzymatic activity or on the presence of a particular small molecule. Certain aspects of the invention also provide methods for cloning genes involved in the synthesis, modification or degradation of a given molecule and for the directed evolution of proteins that perform a specified enzymatic function. Certain methods of the invention can be used to isolate the genes responsible for directing the biosynthesis, modification or degradation of a particular target molecule and to isolate polypeptide variants having new or improved enzymatic activity.
    Type: Application
    Filed: September 30, 2002
    Publication date: April 3, 2003
    Inventor: Justin Gallivan
  • Pollen-specific novel calmodulin-binding protein, NPG1 (No Pollen Germination1), promoter, coding sequences and methods for using the same
    Publication number: 20030061635
    Abstract: The present disclosure describes a novel calmodulin-binding protein, termed NPG1 (No Pollen Germination1) of plants that is specifically expressed in pollen, and the nucleic acid coding sequence, and genomic DNA fragments containing pollen-specific transcriptional and translational regulatory elements. Genetic, histological, and pollen germination studies with the Arabidopsis mutant line for the NPG1 gene indicate that NPG1 is essential for pollen germination. Therefore, this invention provides new means to generate a transgenic plant that is male sterile by modulating expression of the NPG1 gene or interfering with the function of the NPG1 polypeptide. The availability of the tissue specific regulatory elements of the NPG1 gene makes it possible for the pollen-specific expression of various genes of interest.
    Type: Application
    Filed: June 20, 2002
    Publication date: March 27, 2003
    Inventors: Anireddy S.N. Reddy, Farida Safadi, Vaka S. Reddy, Maxim Golovkin
  • Hybrid protein for inhibiting the degranulation of mastocytes and the use thereof
    Publication number: 20030059912
    Abstract: A hybrid protein contains a protein that binds to a receptor of mastocytes and basophils and is endocyted by them. The protein can be IgE; IgE fragment; IgE Fc fragment; antibody against IgE receptor of mastocytes and basophils; fragment of the antibody against the IgE receptor of mastocytes and basophils; antibody against mastocyte specific potassium channel; and mast cell degranulating peptide. The hybrid protein also contains a protease cleaving proteins of the secretion process of the mastocytes and basophils so as to inhibit the secretion process without killing the mastocytes and basophils.
    Type: Application
    Filed: August 27, 2002
    Publication date: March 27, 2003
    Applicant: BioteCon Gesellschaft fur biotechnologische Entwicklung und consulting mbH
    Inventors: Hans Bigalke, Jurgen Frevert
  • Treatment of lupus erythematosus
    Patent number: 6537968
    Abstract: The present invention provides for the treatment of an individual suffering from lupus erythematosus utilizing a serine protease inhibitor. The treatment includes the use of a corticosteroid that is administered separately or in combination. The serine proteases preferred are alpha 1-antitrypsin, secretory leucocyte protease inhibitor, alpha 2-macroglobulin or mixtures thereof.
    Type: Grant
    Filed: July 24, 2000
    Date of Patent: March 25, 2003
    Assignee: Alphamed Pharmaceuticals Corp
    Inventors: Darren Lezdey, Jarett Lezdey
  • METHOD FOR DETECTING HIV ANTIBODIES AND ANTIGENS USED TO THIS END
    Publication number: 20030054336
    Abstract: The invention concerns a process of the detection of HIV antibodies against HIV by means of an immunoassay wherein at least one antigen of the env gene product gp41 of an HIV1-subtype-D isolate and at least one antigen derived from gp41 of a different HIV1-subtype of the M group is used and/or at least one antigen of gp41 of an HIV1-subtype-E isolate and at least one antigen derived from gp41 of a different HIV1-subtype isolate of the M group. The invention additionally concerns antigens and antigen mixtures with components derived from gp41 of the HIV1-subtype-D isolate and from gp41 of the HIV1-subtype-E isolate, respectively, as well as their use for the detection of HIV antibodies and a reagent kit.
    Type: Application
    Filed: February 8, 2000
    Publication date: March 20, 2003
    Inventors: FREDERIC DONIE, ELKE FAATZ, EVA HOESS
  • Mammalian TNF- &agr; convertase
    Patent number: 6531290
    Abstract: The present invention provides isolated human and bovine TNF-&agr; convertases, nucleic acids and recombinant vectors encoding the same, host cells comprising the nucleic acids and vectors, and methods for making the convertases using the host cells. This invention further provides antibodies and antigen binding fragments thereof which specifically bind to the convertases and are useful for treating medical conditions caused or mediated by TNF-&agr;. Also provided are screening methods for identifying specific inhibitors of mammalian TNF-&agr; convertases, and for identifying nucleic acids encoding such convertases.
    Type: Grant
    Filed: October 17, 2001
    Date of Patent: March 11, 2003
    Assignee: Schering Corporation
    Inventors: Barbara Dalie, Xuedong Fan, Daniel Lundell, Charles A. Lunn, Jimmy C. Tan, Paul J. Zavodny
  • Zinc finger proteins for DNA binding and gene regulation in plants
    Publication number: 20030044957
    Abstract: Disclosed herein are modified plant zinc finger proteins; compositions comprising modified plant zinc finger proteins and methods of making and using modified plant zinc finger proteins. The modified plant zinc finger proteins, in contrast to naturally-occurring plant zinc finger proteins, have a binding specificity that is determined by tandem arrays of modular zinc finger binding units.
    Type: Application
    Filed: January 22, 2002
    Publication date: March 6, 2003
    Inventors: Andrew Jamieson, Guofu Li
  • Proteases from gram positive organisms
    Publication number: 20030045438
    Abstract: The present invention relates to the identification of a novel metalloprotease in gram positive microorganisms. The present invention provides the nucleic acid and amino acid sequences for the metalloprotease. The present invention also provides host cells having a mutation or deletion of part or all of the gene encoding the metalloprotease. The present invention provides host cells which further comprises a nucleic acid encoding desired heterologous proteins such as enzymes. The present invention also provides cleaning compositions, animal feeds and compositions used to treat a textile that include the metalloprotease of the present invention.
    Type: Application
    Filed: July 2, 2002
    Publication date: March 6, 2003
    Inventor: David A. Estell
  • Anti-inflammatory peptides derived from C-reactive protein
    Patent number: 6528618
    Abstract: A peptide corresponding to positions 62-71 of the sequence of human C-reactive protein (CRP) of the formula: Glu62-Ile-Leu-Ile-Phe-Ser-Lys-ASP-Ile71 and modifications thereof obtained by substitution, deletion, or addition of amino acids, amidation of the C-terminal or acylation of the N-terminal, are capable of inhibiting in vitro the enzymatic activity of human Leukocyte Elastase (hLE) and/or of human Cathepsin G (hCG) and can be used for the treatment of chronic inflammation conditions such as rheumatoid arthritis, pulmonary emphysema, cystic fibrosis, bronchitis, asthma and acute respiratory distress syndrome.
    Type: Grant
    Filed: March 28, 2000
    Date of Patent: March 4, 2003
    Assignee: Yeda Research and Development Co. Ltd.
    Inventors: Matityahu Fridkin, Eran Yavin
  • Protease for activating clotting factor VII
    Patent number: 6528299
    Abstract: A protease for activating the blood clotting factor VII, which is inhibited by the presence of aprotinin, is increased in its activity by calcium ions and/or heparin or heparin-related substances, and in SDS-PAGE, on subsequent staining in the non-reduced state, comprises one or more bands in the molecular weight range from 50 to 75 kDa; and in SDS-PAGE, on subsequent staining in the reduced state, comprises a band at 40 to 55 kDa, one or more bands in the molecular weight range from 10 to 35 kDa, and a band in the molecular weight range between 60 and 65 kDa, which corresponds to a proenzyme. Pharmaceutical preparations containing the protease or its proenzyme are suitable for the prophylaxis and treatment of bleeding events, e.g. in the presence of FVIII inhibitors, wound healing and for the treatment of disorders which are caused by fibrin-containing thrombin.
    Type: Grant
    Filed: April 21, 1999
    Date of Patent: March 4, 2003
    Assignee: Aventis Behring GmbH
    Inventors: Jürgen Römisch, Annette Feussner, Hans-Arnold Stöhr
  • Fibrinogenolytic proteases with thrombolytic and antihypertensive activities: medical application and novel process of expression and production
    Publication number: 20030039644
    Abstract: The present invention provides a process of producing highly purified proteases. The invention further provides the use of such purified proteases in treating cardiovascular disorders, including hypertension, stroke and thrombosis.
    Type: Application
    Filed: August 24, 2001
    Publication date: February 27, 2003
    Applicant: Academia Sinica
    Inventor: Shyh-Horng Chiou
  • Enzyme and DNA sequence encoding krill-derived multifunctional protein
    Patent number: 6524814
    Abstract: The present invention provides nucleic acid and corresponding amino acid sequences of a multifunctional protein that has been found to be useful in numerous medical and cosmetic contexts. A protein having “multifunctional activity,” is defined herein as including at least one of a chymotrypsin, trypsin, collagenase, elastase or exo peptidase activity or asialo GM1 ceramide binding activity. These proteins are useful for multiple purposes, including treating viral infections such as herpes outbreaks, fungal, bacterial or parasitic infections, including the primary and secondary infections of leprosy, colitis, ulcers, hemorrhoids, corneal scarring, dental plaque, acne, cystic fibrosis, blood clots, wounds, immune disorders including autoimmune disease and cancer.
    Type: Grant
    Filed: October 26, 1999
    Date of Patent: February 25, 2003
    Assignee: Phairson Medical, Inc.
    Inventors: John Kay, Peter Kille
  • Modified interleukin-1&bgr; converting enzyme with increased stability
    Patent number: 6524807
    Abstract: Modified forms of human interleukin-1&bgr; converting enzyme (ICE) that display proteolytic activity and, furthermore, have increased stability compared to unmodified human ICE are disclosed. Nucleic acid molecules encoding a modified p10 subunit of ICE, and recombinant vectors and host cells incorporating such nucleic acid molecules, are also disclosed. A modified ICE protein of the invention can be used to cleave proteolytically ICE substrates and to identify modulators of ICE activity in screening assays. Moreover, due to its enhanced stability, the modified ICE of the invention is particularly suitable for use in the preparation of ICE crystals for X-ray crystallography.
    Type: Grant
    Filed: April 6, 2001
    Date of Patent: February 25, 2003
    Assignee: BASF Aktiengellschaft
    Inventors: Robert V. Talanian, John A. Mankovich, Tariq Ghayur, Catherine R. Ferenz
  • Human endothelin converting enzyme-like proteins and polynucleotides encoding the same
    Patent number: 6524840
    Abstract: Novel human polynucleotide and polypeptide sequences are disclosed that can be used in therapeutic, diagnostic, and pharamacogenomic applications.
    Type: Grant
    Filed: September 22, 2000
    Date of Patent: February 25, 2003
    Assignee: Lexicon Genetics Incorporated
    Inventors: Gregory Donoho, C. Alexander Turner, Jr., Michael C. Nehls, Glenn Friedrich, Brian Zambrowicz, Arthur T. Sands
  • Nucleic acids encoding aggrecan degrading metallo proteases
    Patent number: 6521436
    Abstract: The invention is directed to the family of aggrecan degrading metallo proteases (ADMPs) that exhibit the ability to cleave the aggrecan core protein between amino acid residues Glu373-Ala374. The invention encompasses the nucleic acids encoding such enzymes, processes for production of recombinant ADMPs, compositions containing such enzymes, and the use of these enzymes in various assays and for the development of novel inhibitors for use as therapies for diseases involving aggrecanase-mediated degradation of cartilage or other aggrecanase-associated diseases.
    Type: Grant
    Filed: August 9, 2000
    Date of Patent: February 18, 2003
    Assignee: Bristol-Myers Squibb Company
    Inventors: Elizabeth C. Arner, Timothy C. Burn, Robert A. Copeland, Carl P. Decicco, Ruiqin Liu, Ronald Magolda, Michael Pratta, Kimberly A. Solomon, Micky D. Tortorella, James M. Trzaskos, Fude Yang
  • Maize proteinase inhibitor-like polynucleotides and methods of use
    Publication number: 20030033632
    Abstract: Methods and compositions for modulating development and defense response are provided. Nucleotide sequences encoding a maize proteinase inhibitor-like protein is provided. Nucleotide sequences comprising the proteinase inhibitor-like promoter are also provided. The sequences can be used in expression cassettes for modulating development, developmental pathways, and the plant defense response. Transformed plants, plant cells, tissues, and seed are also provided.
    Type: Application
    Filed: October 23, 2001
    Publication date: February 13, 2003
    Applicant: Pioneer Hi-Bred International, Inc.
    Inventors: Virginia C. Crane, Carl R. Simmons
  • Isolated human protease proteins, nucleic acid molecules encoding human protease proteins, and uses thereof
    Patent number: 6518055
    Abstract: The present invention provides amino acid sequences of peptides that are encoded by genes within the human genome, the protease peptides of the present invention. The present invention specifically provides isolated peptide and nucleic acid molecules, methods of identifying orthologs and paralogs of the protease peptides, and methods of identifying modulators of the protease peptides.
    Type: Grant
    Filed: March 26, 2001
    Date of Patent: February 11, 2003
    Assignee: Applera Corporation
    Inventors: Weiniu Gan, Jane Ye, Valentina Di Francesco, Ellen M. Beasley
  • Metallo-endopeptidases
    Patent number: 6518054
    Abstract: The present invention provides genes encoding variants of metallo-endopeptidases that have been engineered to be resistant to prolonged boiling while having maintained their enzymatic performance at much lower temperatures. In addition, thermal stability of the metallo-endopeptidases is highly dependent on calcium at concentrations in the mM range. The invention further provides active metallo-endopeptidases variants whose stability depending on calcium concentration can be changed so as to provide metallo-endopeptidases that are calcium dependent or independent. The invention also provides genes that encode boiling-resistant metallo-endopetidases whose stability depending on calcium concentration can be changed. The invention also provides vectors and cells comprising these genes and proteases produced through these genes, vectors and/or cells.
    Type: Grant
    Filed: December 7, 1999
    Date of Patent: February 11, 2003
    Assignee: Rijksuniverstteit Te Groningen
    Inventors: Lambertus Van den Burg, Oene Robert Veltman, Gerard Venema
  • Hepatitis C virus protease
    Publication number: 20030027317
    Abstract: The protease necessary for polyprotein processing in Hepatitis C virus is identified, cloned, and expressed. Proteases, truncated protease, and altered proteaces are disclosed which are useful for cleavage of specific polypeptides, and for assay and design of antiviral agents specific for HCV.
    Type: Application
    Filed: June 18, 2001
    Publication date: February 6, 2003
    Inventors: Michael Houghton, Qui-Lim Choo, George Kuo
  • Enzymatic array and process of making same
    Publication number: 20030027298
    Abstract: An enzymatic array is provided, which composition comprises one or more enzymes non-covalently bound to a peptide backbone, wherein at least one of the enzymes is heterologous to the peptide backbone and the peptide backbone is capable of having bound thereto a plurality of enzymes. The array is useful, for example, in recovery systems, targeted multi-enzyme delivery systems, soluble substrate modification, quantification type assays, and other applications in the food industry, feed, textiles, bioconversion, pulp and paper production, plant protection and pest control, wood preservatives, topical lotions and biomass conversions.
    Type: Application
    Filed: September 17, 2001
    Publication date: February 6, 2003
    Inventors: Richard R. Bott, Kathleen A. Clarkson, Timothy Fowler, Chung-Cheng Liu, Michael Ward, Hai-Ying Xia
  • Bacterial promoters and methods of use
    Publication number: 20030027286
    Abstract: Compositions and methods are disclosed herein that relate to the development of fusion promoters for regulating gene expression in bacteria. Embodiments include fusion promoters comprising one or more operators linked to a promoter that is modified to have altered activity in Gram-positive organisms. Vectors and cells containing these fusion promoters are also described. Other embodiments include, methods of using these fusion promoters to regulate nucleic acid and/or polypeptide expression, methods of using these fusion promoters to identify proliferation-required genes, and methods of using these fusion promoters to identify molecules having potential antibiotic activity.
    Type: Application
    Filed: December 21, 2001
    Publication date: February 6, 2003
    Inventors: Robert Haselbeck, Daniel Wall, Molly Gross
  • Moss genes from Physcomitrella patens encoding proteins involved in the regulation of cell division, growth and biomass formation in plants
    Publication number: 20030024003
    Abstract: Isolated nucleic acid molecules, designated GDRP nucleic acid molecules, which encode novel GDRPs from Physcomitrella patens are described. The invention also provides antisense nucleic acid molecules, recombinant expression vectors containing GDRP nucleic acid molecules, and host cells and organisms into which the expression vectors have been introduced. The invention still further provides isolated GDRPs, mutated GDRPs, fusion proteins, antigenic peptides and methods for the improvement of production of a desired compound from transformed cells based on genetic engineering of GDRP genes in this organism.
    Type: Application
    Filed: January 25, 2001
    Publication date: January 30, 2003
    Inventors: Markus Frank, Andreas Reindl, Ralf-Michael Schmidt, Annette Freund, Thomas Ehrhardt, Friedrich Bischoff, Andreas Renz, Elke Duwenig, Petra Cirpus, Jens Lerchl, Ralf Reski
  • Lawsonia intracellularis proteins, and related methods and materials
    Publication number: 20030021802
    Abstract: Isolated polynucleotide molecules contain a nucleotide sequence that encodes a L. intracellularis HtrA, PonA, HypC, LysS, YcfW, ABC1, or Omp100 protein, a substantial portion of the sequences, or a homologous sequence. Related polypeptides, immunogenic compositions and assays are described.
    Type: Application
    Filed: August 1, 2002
    Publication date: January 30, 2003
    Inventor: Everett L. Rosey
  • Gene controlling fruit size and cell division in plants
    Publication number: 20030024013
    Abstract: The present invention relates to the isolation and identification of a nucleic acid molecule which regulates fruit size and/or cell division in plants and the protein encoded by such a nucleic acid molecule. The invention also relates to an expression vector containing the encoding nucleic acid and methods whereby fruit size is reduced and/or increased and cell division is regulated by transformation of plants with the disclosed nucleic acid molecule. Host cells as well as transgenic plants and plant seeds containing the nucleic acid molecule of the present invention are also discussed.
    Type: Application
    Filed: July 3, 2001
    Publication date: January 30, 2003
    Inventor: Steven D. Tanksley
  • Virulence-associated nucleic acid sequences and uses thereof
    Publication number: 20030022349
    Abstract: Disclosed are bacterial virulence polypeptides and nucleic acid sequences (e.g., DNA) encoding such polypeptides, and methods for producing such polypeptides by recombinant techniques. Also provided are methods for utilizing such polypeptides to screen for antibacterial or bacteriostatic compounds.
    Type: Application
    Filed: October 10, 2001
    Publication date: January 30, 2003
    Inventors: Frederick M. Ausubel, Laurence G. Rahme
  • Use of calcium-activated neutral protease (CANP) inhibitors in pharmaceutical preparations
    Patent number: 6511959
    Abstract: The invention provides to the use of inhibitors of calcium-activated neutral proteases (CANPs) and their pharmaceutically acceptable addition salts or their active subunits in the field of tumor therapy, especially cancer therapy. The inhibitors can especially be used against protease-bound bound glycosaminoglycan substrate-dependent tumors and meiotic cell division. The inhibitors include heat-stable, tetrameric proteins of approximately 240 kD possessing active subunits, and tripeptides exhibiting inhibitory activity. The pharmaceutical compositions may be prepared in a manner known per se, with carriers or additives commonly used in the pharmaceutical industry.
    Type: Grant
    Filed: July 6, 2000
    Date of Patent: January 28, 2003
    Assignee: Norsk Hydro AS
    Inventor: Helen Logothetou-Rella
  • Human proteases and polynucleotides encoding the same
    Patent number: 6509456
    Abstract: Novel human polynucleotide and polypeptide sequences are disclosed that can be used in therapeutic, diagnostic, and pharmacogenomic applications.
    Type: Grant
    Filed: February 9, 2001
    Date of Patent: January 21, 2003
    Assignee: Lexicon Genetics Incorporated
    Inventors: Gregory Donoho, John Scoville, C. Alexander Turner, Jr., Glenn Friedrich, Alejandro Abuin, Brian Zambrowicz, Arthur T. Sands
  • Pichia secretory leader for protein expression
    Publication number: 20030013154
    Abstract: Polynucleotides, vectors and host cells comprising a polynucleotide having a fragment of a leader sequence and a second nucleotide sequence that encodes a polypeptide heterologous to the leader sequence, wherein the leader sequence fragment is sufficient for secretion and comprises an amino acid sequence that comprises at least about 70% sequence identity to the leader sequence of Pichia acaciae killer toxin, wherein the heterologous polypeptide is not naturally contiguous to the leader sequence, and wherein upon expression of the polynucleotide molecule in a host cell suitable for expression thereof, the heterologous polypeptide is produced that is free of additional N-terminal amino acids.
    Type: Application
    Filed: June 25, 2002
    Publication date: January 16, 2003
    Applicant: Chiron Corporation
    Inventors: Kenneth Crawford, Isabel Zaror, Robert J. Bishop, Michael A. Innis
  • Probe for analyzing protein-protein interaction and method of analyzing protein-protein interactions with the use of the same
    Publication number: 20030003506
    Abstract: A probe for protein-protein interaction analysis applicable to various proteins, which enables simple analysis in high accuracy, and a method for analyzing interaction of two proteins is provided. Protein splicing from the probe is caused by protein-protein interaction, and a physiochemically or biochemically detectable protein is regenerated.
    Type: Application
    Filed: April 16, 2002
    Publication date: January 2, 2003
    Inventors: Yoshio Umezawa, Takeaki Ozawa