Bacillus Subtilus Or Bacillus Lichenoformis Patents (Class 435/222)
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Phosphate-free automatic dishwashing detergent compositions having a protease and a complexing agent
Patent number: 11834634Abstract: A phosphate-free automatic dishwashing cleaning composition comprising i) a protease wherein the protease is a variant having at least 60% identity with the amino acid sequence of SEQ ID NO:1 and comprising at least one amino acid substitution (using the SEQ ID NO: 1 numbering) selected from the group consisting of X54T; X126A, D, G, V, E, K, I; X127E, S, T, A, P, G, C; and X128E, C, T, D, P, G, L, Y, N; and ii) from 10 to 50% by weight of the composition of a complexing agent system comprising from 0 to less than 30% by weight of the composition of citric acid.Type: GrantFiled: December 12, 2018Date of Patent: December 5, 2023Assignee: The Procter & Gamble CompanyInventors: Michelle Jackson, Eva Maria Perez-Prat de Vinuesa, David John Tarbit, Philip Frank Souter, Lilia Maria Babe, David Aaron Estell, Frits Goedegebuur, Harm Jan Mulder, Sina Pricelius, Lydia Dankmeyer, Thijs Kaper, Hatice Billur Engin -
Patent number: 11473075Abstract: The present disclosure provides AprL-clade protease enzymes, including variant AprL-clade protease enzymes, nucleic acids encoding same, and compositions and methods related to the production and use thereof, including an AprL-clade variant subtilisin enzyme that has improved stability and/or soil removal compared to a parent AprL-clade subtilisin enzyme.Type: GrantFiled: December 19, 2019Date of Patent: October 18, 2022Assignee: DANISCO US INC.Inventors: David A. Estell, Frits Goedegebuur, Marc Anton Bernhard Kolkman, Rie Mejldal, Katherine Augustyn, Lilia Maria Babe, Richard R Bott, Jian Yao, Roopa Santosh Ghirnikar
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Patent number: 11015183Abstract: The present invention relates to novel subtilase variants exhibiting increased stability and preferably on par or improved wash performance. The variants of the invention are suitable for use in e.g. cleaning or detergent compositions, such as laundry detergent compositions and dish wash compositions, including automatic dish wash compositions. The present invention also relates to isolated DNA sequences encoding the variants, expression vectors, host cells, and methods for producing and using the variants of the invention.Type: GrantFiled: December 16, 2019Date of Patent: May 25, 2021Assignee: NOVOZYMES A/SInventors: Frank Winther Rasmussen, Peter Kamp Hansen, Lars Lehman Hylling Christensen
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Method for efficiently expressing pullulanase in bacillus subtilis and recombinant bacillus subtilis
Patent number: 10975366Abstract: The present invention relates to the field of genetic engineering, particularly to a method for efficiently expressing pullulanase in Bacillus subtilis and recombinant Bacillus subtilis. said method includes steps of constructing modified Bacillus subtilis strain with deletion of alkaline protease gene and neutral protease gene, constructing expression vector including an optimized combination of promoter and signal peptide and pullulanase gene, and transforming said modified Bacillus subtilis strain with by said expression vector. A series of combinations of promoter and signal peptide are optimized to obtain the combination for efficiently expressing pullulanase, provide an industrial application basis.Type: GrantFiled: October 24, 2017Date of Patent: April 13, 2021Assignee: GUANGDONG VTR BIO-TECH CO., LTDInventors: Yangyuan Li, Jianrong Wang, Ling Yang, Man Qu, Lizhi Chen, Jiale Huang -
Patent number: 10563189Abstract: The present invention provides variant subtilisins and compositions comprising at least one variant subtilisin set forth herein, as well as methods for using these variants and compositions. In some embodiments, the present invention provides variant subtilisins suitable for laundry cleaning applications.Type: GrantFiled: November 10, 2014Date of Patent: February 18, 2020Assignee: The Procter & Gamble CompanyInventors: Joshua R. Basler, Luis G. Cascao-Pereira, David A. Estell, James T. Kellis, Jr.
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Patent number: 10093911Abstract: The present invention relates to subtilisin variants and methods for obtaining subtilisin variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.Type: GrantFiled: February 14, 2013Date of Patent: October 9, 2018Assignee: Novozymes A/SInventors: Werner Besenmatter, Marco Malten, Benie Astrid
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Patent number: 9357786Abstract: Polypeptide having activity inducing pathogen plant defense, nucleotide sequence encodes it, microorganism, compositions and methods. The polypeptide is a subtilisin derived from a strain of Acremonium strictum. The polypeptide is encoded by the nucleotide sequence SEQ ID NO: 1 or sequences at least 90% homologous to the same and has the amino acid sequence SEQ ID NO: 2 or sequences at least 90% homologous to the same.Type: GrantFiled: March 16, 2012Date of Patent: June 7, 2016Assignees: UNIVERSIDAD NACIONAL DE TUCUMAN, CONSEJO NACIONAL DE INVESTIGACIONES CIENTIFICAS Y TECNICAS (CONICET)Inventors: Atilio Pedro Castagnaro, Juan Carlos Diaz Ricci, Nadia Regina Chalfoun, Josefina Racedo, Sergio Miguel Salazar
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Patent number: 9200239Abstract: Subtilase variants having an improved wash performance on egg stains. These subtilases are useful exhibiting excellent or improved wash performance on egg stains when used in e.g. cleaning or detergent compositions, such as laundry detergent compositions and dish wash compositions, including automatic dish wash compositions.Type: GrantFiled: June 5, 2014Date of Patent: December 1, 2015Assignee: Novozymes A/SInventors: Stefan Minning, Jurgen Carsten Franz Knotzel, Niels Henrik Sorensen
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Patent number: 9169475Abstract: The present invention relates to methods for producing variants of a parent TY145 subtilase and of a parent BPN? subtilase and to TY145 and BPN? variants having altered properties as compared to the parent TY145/BPN? subtilase.Type: GrantFiled: August 29, 2013Date of Patent: October 27, 2015Assignee: Novozymes A/SInventors: Allan Svendsen, Henriette Draborg
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Patent number: 9096838Abstract: The present invention provides engineered protease variants. In particular, the protease variants comprise combinable mutations at selected surface positions that affect the charge and/or hydrophobicity of the enzyme to enhance at least one desired property of the resulting variant enzyme in a chosen application. Compositions comprising the protease variants, and methods for using the same are also provided.Type: GrantFiled: May 2, 2014Date of Patent: August 4, 2015Assignee: Danisco US Inc.Inventors: Luis G. Cascao-Pereira, David A. Estell, James T. Kellis, Jr.
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Patent number: 9062291Abstract: A transglutaminase protein which is mutated to have improved heat resistance and/or pH stability. A mutation is introduced into WT transglutaminase at a cysteine residue capable of forming a disulfide bond (SS bond).Type: GrantFiled: August 14, 2009Date of Patent: June 23, 2015Assignee: AJINOMOTO CO., INC.Inventors: Keiichi Yokoyama, Mototaka Suzuki, Tatsuki Kashiwagi, Eiichiro Suzuki, Masayo Date, Seiichi Taguchi
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Publication number: 20150031589Abstract: The present invention provides methods for protein engineering and serine protease variants produced there from. Specifically, the present invention provides serine protease variants having one or more substitutions as compared to a reference serine protease. In addition, the present invention provides compositions comprising these serine protease variants. In some embodiments, the present invention provides cleaning compositions comprising at least one of these serine protease variants.Type: ApplicationFiled: July 21, 2014Publication date: January 29, 2015Applicant: DANISCO US INC.Inventors: Wolfgang Aehle, Luis G. Cascao-Pereira, David A. Estell, Frits Goedegebuur, James T. Kellis, JR., Ayrookaran J. Poulose, Brian F. Schmidt
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Publication number: 20140342433Abstract: The present invention relates to subtilase variants and methods for obtaining subtilase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.Type: ApplicationFiled: November 23, 2012Publication date: November 20, 2014Inventors: Allan Svendsen, Marco Malten, Christian Lundager Gylstorff
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Patent number: 8889395Abstract: A method for producing a crystalline and/or amorphous metabolite suspension from a cell fermentation broth in a continuous centrifuge process comprising (a) adding at a separate inlet to the centrifuge the fermentation broth comprising the cells and the metabolite of interest, wherein the metabolite is partly or wholly on crystalline and/or amorphous form; (b) adding at another inlet to the centrifuge an aqueous liquid comprising a salt and/or a carbohydrate having a higher density than the cells and a lower density than the metabolite of interest in its precipitated form; (c) removing the cells at a separate outlet to the centrifuge; and (d) removing the suspension comprising the crystalline and/or amorphous metabolite of interest at another outlet to the centrifuge.Type: GrantFiled: March 25, 2011Date of Patent: November 18, 2014Assignee: Novozymes A/SInventors: Peter Frode Pind, Simon Glanville, Sune Jakobsen, Svend Kaasgaard
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Patent number: 8889396Abstract: The present invention provides methods of improving growth performance, improving the efficiency of feed utilization, increasing feed digestibility, and decreasing mortality of immature and developing animals receiving animal feed. Methods of producing a crude keratinise enzyme extract and animal feed supplements for achieving the same are also provided.Type: GrantFiled: October 23, 2013Date of Patent: November 18, 2014Assignee: North Carolina State UniversityInventor: Jason C. H. Shih
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Publication number: 20140335596Abstract: The present invention relates to protease variants and methods for obtaining protease variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.Type: ApplicationFiled: December 18, 2012Publication date: November 13, 2014Inventors: Werner Besenmatter, Astrid Benie, Esben Peter Friis, Pernille Ollendorf Micheelsen
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Patent number: 8883444Abstract: The invention relates to a method for synthesizing a peptide by enzymatically preparing an ester or thioester from (i) an N-terminal protected amino acid or an N-terminal protected peptide where either can have a protected C-terminal ester group and (ii) an alcohol represented by the formula HO—CX2—Z or a thiol represented by the formula HS—CX2—Z, each X independently representing a halogen atom or a hydrogen atom; and Z represents an electron withdrawing group comprising at least one sp3-hybridized carbon comprising at least two substituents comprising a heteroatom directly attached to the at least one sp3-hybridized carbon or at least one sp2-hybridized carbon comprising one or two substituents comprising a heteroatom directly attached to the at least one sp2-hybridized carbon, and enzymatically coupling the prepared ester or thioester with an optionally C-terminal protected amino acid or with an optionally C-terminal protected peptide in a medium comprising 2 wt. % water or less.Type: GrantFiled: November 19, 2009Date of Patent: November 11, 2014Assignee: Enzypep B.V.Inventors: Peter Jan Leonard Mario Quaedflieg, Timo Nuijens, Claudia Cusan, Catharina Hubertina Maria Schepers
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Publication number: 20140308736Abstract: The present invention provides engineered protease variants. In particular, the protease variants comprise combinable mutations at selected surface positions that affect the charge and/or hydrophobicity of the enzyme to enhance at least one desired property of the resulting variant enzyme in a chosen application. Compositions comprising the protease variants, and methods for using the same are also provided.Type: ApplicationFiled: May 2, 2014Publication date: October 16, 2014Applicant: DANISCO US INC.Inventors: Luis G. Cascao-Pereira, David A. Estell, James T. Kellis, JR.
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Publication number: 20140302590Abstract: The present invention provides protease compositions particularly suited for dishwashing applications.Type: ApplicationFiled: April 7, 2014Publication date: October 9, 2014Applicant: Danisco US Inc.Inventors: Pieter Augustinus, Frits Goedegebuur, Ayrookaran J. Poulose, Johannes C. Van Der Laan
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Publication number: 20140294881Abstract: The present invention provides novel protein variants that exhibit reduced immunogenic responses, as compared to the parental proteins. The present invention further provides DNA molecules that encode novel variants, host cells comprising DNA encoding novel variants, as well as methods for making proteins less allergenic. In addition, the present invention provides various compositions that comprise these proteins that are less immunogenic than the wild-type proteins.Type: ApplicationFiled: September 18, 2008Publication date: October 2, 2014Applicant: Danisco US Inc.Inventors: David A. Estell, Fiona A. Harding, Ayrookaran J. Poulose
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Publication number: 20140295527Abstract: The present invention provides a Bacillus sp. subtilisin variant. In addition, the present invention provides automatic dishwashing compositions comprising this serine protease variant.Type: ApplicationFiled: April 22, 2014Publication date: October 2, 2014Applicant: DANISCO US INC.Inventors: David A. Estell, Frits Goedegebuur, Ayrookaran J. Poulose
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Patent number: 8802416Abstract: The present invention relates to a thermostable protease useful as an enzyme for industrial use, a gene encoding the same and a method of producing the enzyme by genetic engineering technique. More particularly, the present invention discloses a thermostable organic solvent tolerant protease and its code gene and application. The invention also discloses a method for preparing protease by isolating from Bacillus subtilis isolate Rand bacteria. The activity and stability of protease (preferably named Rand protease) at high temperature, and can be used in fields of washing agent industry, foodstuff industry, biological pharmacy and environmental biological technique.Type: GrantFiled: October 7, 2010Date of Patent: August 12, 2014Assignee: Universiti Putra MalaysiaInventors: Raja Noor Zaliha Raja Abd Rahman, Abu Bakar Salleh, Mahiran Basri, Randa Abdelkareem Abusham
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Publication number: 20140212385Abstract: Polypeptide having activity inducing pathogen plant defence, nucleotide sequence encodes it, microorganism, compositions and methods. The polypeptide is a subtilisin derived from a strain of Acremonium strictum. The polypeptide is encoded by the nucleotide sequence SEQ ID No.: 1 or sequences at least 90% homologous to the same and has the amino acid sequence SEQ ID No: 2 or sequences at least 90% homologous to the same.Type: ApplicationFiled: March 16, 2012Publication date: July 31, 2014Applicant: CONSEJO NACIONAL DE INVESTIGACIONES CIENTIFICAS Y TECNICAS (CONICET)Inventors: Atilio Pedro Castagnaro, Juan Carlos Diaz Ricci, Nadia Regina Chalfoun, Josefina Racedo, Sergio Miguel Salazar
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Publication number: 20140199752Abstract: A method of producing an enzyme of interest in a fed-batch cultivation comprising: a) cultivating a microorganism in a culture medium conducive to its growth wherein the microorganism produces the enzyme of interest; and b) adding a manganese compound to the culture medium one or more times during the cultivation.Type: ApplicationFiled: August 3, 2012Publication date: July 17, 2014Applicant: NOVOZYMES A/SInventors: Jon Martin Persson, Niels Banke
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Patent number: 8753861Abstract: The present invention provides engineered protease variants. In particular, the protease variants comprise combinable mutations at selected surface positions that affect the charge and/or hydrophobicity of the enzyme to enhance at least one desired property of the resulting variant enzyme in a chosen application. Compositions comprising the protease variants, and methods for using the same are also provided.Type: GrantFiled: November 10, 2009Date of Patent: June 17, 2014Assignee: Danisco US Inc.Inventors: Luis G. Cascao-Pereira, David A. Estell, James T. Kellis, Jr.
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Publication number: 20140073552Abstract: The present invention relates to enzymes produced by mutating the genes for a number of subtilases and expressing the mutated genes in suitable hosts are presented. The enzymes exhibit improved stability and/or improved wash performance in any detergent in comparison to their wild type parent enzymes. The enzymes are well-suited for use in any detergent and for some in especially liquid or solid shaped detergent compositions.Type: ApplicationFiled: October 1, 2012Publication date: March 13, 2014Applicants: UNILEVER PLC, NOVOZYMES A/SInventors: Laurens Nicolaas Sierkstra, Jan Klugkist, Peter Markvardsen, Claus Von Der Osten, Peter Bauditz
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Patent number: 8642313Abstract: The present invention provides methods of improving growth performance, improving the efficiency of feed utilization, increasing feed digestibility, and decreasing mortality of immature and developing animals receiving animal feed. Methods of producing a crude keratinase enzyme extract and animal feed supplements for achieving the same are also provided.Type: GrantFiled: February 25, 2011Date of Patent: February 4, 2014Assignee: North Carolina State UniversityInventor: Jason C. H. Shih
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Publication number: 20140011234Abstract: The present invention provides cells that have been genetically manipulated to have an altered capacity to produce expressed proteins. In particular, the present invention relates to Gram-positive microorganisms, such as Bacillus species having enhanced expression of a protein of interest, wherein one or more chromosomal genes have been inactivated, and preferably wherein one or more chromosomal genes have been deleted from the Bacillus chromosome. In some further embodiments, one or more indigenous chromosomal regions have been deleted from a corresponding wild-type Bacillus host chromosome.Type: ApplicationFiled: January 18, 2013Publication date: January 9, 2014Applicant: DANISCO US INC.Inventors: Eugenio Ferrari, Carole Harbison, M. Harunur Rashid, Walter Weyler
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Patent number: 8623630Abstract: The present invention provides methods of altering the production of desired polypeptides in a host cell. In particular, the present invention provides polynucleotides encoding truncated SecG proteins capable of facilitating the secretion of desired proteases by a bacterial host cell, such as Bacillus species, as well as expression vectors and a host cell containing the polynucleotides.Type: GrantFiled: October 15, 2012Date of Patent: January 7, 2014Assignee: Danisco US Inc.Inventors: Anita Van Kimmenade, Carol Fioresi, Caroline Peres, Eugenio Ferrari
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Publication number: 20130344568Abstract: The present invention relates to methods for producing variants of a parent TY145 subtilase and of a parent BPN? subtilase and to TY145 and BPN? variants having altered properties as compared to the parent TY145/BPN? subtilase.Type: ApplicationFiled: August 29, 2013Publication date: December 26, 2013Applicant: Novozymes A/SInventors: Allan Svendsen, Henriette Draborg
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Publication number: 20130323816Abstract: The present invention provides protease compositions particularly suited for dishwashing applications.Type: ApplicationFiled: April 25, 2013Publication date: December 5, 2013Applicant: DANISCO US INC.Inventors: Pieter Augustinus, Frits Goedegebuur, Ayrookaran J. Poulose, Johannes C. Van Der Laan
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Patent number: 8524223Abstract: A microorganism inhibits the growth of various plant pathogens, and is not reduced in its efficacy even when the microorganism is used in combination with a chemical pesticide. The microorganism is Bacillus subtilis KS1 strain (NITE BP-569). The plant disease control agent comprises a culture of the microorganism as an active ingredient.Type: GrantFiled: July 3, 2009Date of Patent: September 3, 2013Assignee: University of YamanashiInventors: Tsutomu Takayanagi, Shunji Suzuki, Seiichi Furuya
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Publication number: 20130216519Abstract: The present invention provides novel protein variants that exhibit reduced immunogenic responses, as compared to the parental proteins. The present invention further provides DNA molecules that encode novel variants, host cells comprising DNA encoding novel variants, as well as methods for making proteins less allergenic. In addition, the present invention provides various compositions that comprise these proteins that are less immunogenic than the wild-type proteins.Type: ApplicationFiled: February 1, 2013Publication date: August 22, 2013Applicant: DANISCO US INC.Inventor: DANISCO US INC.
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Publication number: 20130164818Abstract: Biomass (e.g., plant biomass, animal biomass, microbial, and municipal waste biomass) is processed to produce useful products, such as food products and amino acids.Type: ApplicationFiled: December 6, 2011Publication date: June 27, 2013Applicant: XYLECO, INC.Inventor: Marshall Medoff
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Publication number: 20130122571Abstract: The present invention relates to methods for producing variants of a parent TY145 subtilase and of a parent BPN? subtilase and to TY145 and BPN? variants having altered properties as compared to the parent TY145/BPN? subtilase.Type: ApplicationFiled: January 17, 2013Publication date: May 16, 2013Applicant: NOVOZYMES A/SInventor: Novozymes A/S
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Publication number: 20130102052Abstract: The present invention provides methods of altering the production of desired polypeptides in a host cell. In particular, the present invention provides polynucleotides encoding truncated SecG proteins capable of facilitating the secretion of desired proteases by a bacterial host cell, such as Bacillus species, as well as expression vectors and a host cell containing the polynucleotides.Type: ApplicationFiled: October 15, 2012Publication date: April 25, 2013Applicant: DANISCO US INC.Inventor: DANISCO US INC.
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Publication number: 20130065223Abstract: The present invention provides a universally applicable lysis buffer comprising a chaotropic 5 agent, a reducing agent, and a proteolytic enzyme suitable for processing a wide variety o different sample types, such as different types of bodily samples relevant for the diagnosis o a respiratory disease. Furthermore, the present invention provides the use of a chaotropic agent, a reducing agent, and a proteolytic enzyme for the lysis of a broad spectrum of bodily samples. Moreover, the present invention provides a method for processing bodily samples which is universally applicable to the lysis of a variety of different types of bodily samples. Furthermore, the present invention provides methods for analyzing a bodily sample or for detecting the presence of a pathogen in a bodily sample, preferably, for diagnosing a respiratory disease, such as pneumonia or tuberculosis.Type: ApplicationFiled: May 9, 2011Publication date: March 14, 2013Applicant: CURETIS AGInventors: Matthias Klein, Gerd Lüdke, Andreas Boos
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Publication number: 20130029402Abstract: The present invention provides host cells that have been genetically manipulated to have an enhanced capacity to produce proteins of interest. In particular, the invention relates to modified Bacillus sp. Host cells that have at least one inactivated phr gene. The enhanced production of proteins of interest by the modified Bacillus sp. Host cells is further increased in modified Bacillus sp. Host cells that overexpress YmaH.Type: ApplicationFiled: September 19, 2012Publication date: January 31, 2013Applicant: DANISCO US INC.Inventor: Danisco US Inc.
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Publication number: 20130017592Abstract: A method for producing a crystalline and/or amorphous metabolite suspension from a cell fermentation broth in a continuous centrifuge process comprising (a) adding at a separate inlet to the centrifuge the fermentation broth comprising the cells and the metabolite of interest, wherein the metabolite is partly or wholly on crystalline and/or amorphous form; (b) adding at another inlet to the centrifuge an aqueous liquid comprising a salt and/or a carbohydrate having a higher density than the cells and a lower density than the metabolite of interest in its precipitated form; (c) removing the cells at a separate outlet to the centrifuge; and (d) removing the suspension comprising the crystalline and/or amorphous metabolite of interest at another outlet to the centrifuge.Type: ApplicationFiled: March 25, 2011Publication date: January 17, 2013Applicant: NOVOZYMES A/SInventors: Peter Frode Pind, Simon Glanville, Sune Jakobsen, Svend Kaasgaard
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Publication number: 20130005637Abstract: Proteases encompassing an amino acid sequence that is at least 70% identical to the amino acid sequence indicated in SEQ ID NO. 1 over its entire length, and exhibit the amino acid substitution I21V in the count in accordance with SEQ ID NO. 1, resp. agents that encompass such proteases, display very good cleaning performance on protease-sensitive stains.Type: ApplicationFiled: March 10, 2011Publication date: January 3, 2013Applicant: HENKEL AG & CO. KGAAInventors: Petra Siegert, Ulrich Schwaneberg, Ronny Martinez, Marion Merkel, Astrid Spitz, Susanne Wieland, Hendrik Hellmuth, Karl-Heinz Maurer
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Publication number: 20120295331Abstract: Subtilase variants having an improved wash performance on egg stains. These subtilases are useful exhibiting excellent or improved wash performance on egg stains when used in e.g. cleaning or detergent compositions, such as laundry detergent compositions and dish wash compositions, including automatic dish wash compositions.Type: ApplicationFiled: July 23, 2012Publication date: November 22, 2012Applicant: NOVOZYMES A/SInventors: Stefan Minning, Jürgen Carsten Franz Knötzel, Niels Henrik Sørensen
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Patent number: 8241885Abstract: The present invention is directed to the identification of a protease prodomain that is capable of binding a corresponding protease with high affinity. The protease prodomain of the present invention is fused to a second protein to form a protease prodomain fusion protein. The presence of a protease prodomain protein in a fusion protein allows for easy and selective purification of the second protein by incubation with the corresponding protease.Type: GrantFiled: September 22, 2010Date of Patent: August 14, 2012Assignee: University of Maryland, College ParkInventor: Philip N. Bryan
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Publication number: 20120165241Abstract: The present invention relates to enzymes produced by mutating the genes for a number of subtilases and expressing the mutated genes in suitable hosts are presented. The enzymes exhibit improved stability and/or improved wash performance in any detergent in comparison to their wild type parent enzymes. The enzymes are well-suited for use in any detergent and for some in especially liquid or solid shaped detergent compositions.Type: ApplicationFiled: March 7, 2012Publication date: June 28, 2012Applicants: UNILEVER PLC, NOVOZYMES A/SInventors: Laurens Nicolaas Sierkstra, Jan Klugkist, Peter Markvardsen, Claus von der Osten, Peter Bauditz
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CHEMICALLY MODIFIED MUTANT SERINE HYDROLASES SHOW IMPROVED CATALYTIC ACTIVITY AND CHIRAL SELECTIVITY
Publication number: 20120156721Abstract: This invention provides novel chemically modified mutant serine hydrolases that catalyze a transamidation and/or a transpeptidation and/or a transesterification reaction. The modified serine hydrolases have one or more amino acid residues in a subsite replaced with a cysteine, wherein the cysteine is modified by replacing the thiol hydrogen in the cysteine with a substituent group providing a thiol side chain comprising a moiety selected from the group consisting of a polar aromatic substituent, an alkyl amino group with a positive charge, and a glycoside. In particularly preferred embodiments, the substitutents include an oxazolidinone, a C1 to C15 alkyl amino group with a positive charge, or a glycoside.Type: ApplicationFiled: February 24, 2012Publication date: June 21, 2012Applicant: The Governing Council of the University of TorontoInventors: John Bryan Jones, Michael Dickman, Richard C. Lloyd -
Publication number: 20120142076Abstract: The present invention provides host cells that have been genetically manipulated to have an enhanced capacity to produce proteins of interest. In particular, the invention relates to modified Bacillus sp. Host cells that have at least one inactivated phr gene. The enhanced production of proteins of interest by the modified Bacillus sp. Host cells is further increased in modified Bacillus sp. Host cells that overexpress YmaH.Type: ApplicationFiled: June 2, 2010Publication date: June 7, 2012Applicant: Danisco US Inc.Inventors: Cristina Bongiorni, Eugenio Ferrari
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Publication number: 20120094358Abstract: Biomass (e.g., plant biomass, animal biomass, microbial, and municipal waste biomass) is processed to produce useful products, such as food products and amino acids.Type: ApplicationFiled: December 6, 2011Publication date: April 19, 2012Applicant: XYLECO, INC.Inventor: Marshall Medoff
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Patent number: 8101563Abstract: The present invention relates to the identification of novel metallo-proteases (MP) in Gram-positive microorganisms. The present invention provides the nucleic acid and amino acid sequences for Bacillus MP. The present invention also provides host cells having a mutation or deletion of part or all of the gene encoding MP. The present invention also provides host cells further comprising nucleic acid encoding desired heterologous proteins such as enzymes. The present invention also provides cleaning compositions comprising an MP of the present invention.Type: GrantFiled: December 8, 2004Date of Patent: January 24, 2012Assignee: Danisco US Inc.Inventor: David A. Estell
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Publication number: 20120009651Abstract: The present invention provides methods and compositions comprising at least one thermolysin-like neutral protease enzyme with improved storage stability and/or catalytic activity. In some embodiments, the thermolysin finds use in cleaning and other applications comprising detergent. In some particularly preferred embodiments, the present invention provides methods and compositions comprising thermolysin formulated and/or engineered to resist detergent-induced inactivation.Type: ApplicationFiled: October 28, 2008Publication date: January 12, 2012Applicant: DANISCO US INC.Inventors: David A. Estell, Ronaldus W.J. Hommes, Amy D. Liu, Andrew Shaw
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Patent number: 8084241Abstract: The present invention relates to methods for producing variants of a parent TY145 subtilase and of a parent BPN? subtilase and to TY145 and BPN? variants having altered properties as compared to the parent TY145/BPN? subtilase.Type: GrantFiled: September 10, 2010Date of Patent: December 27, 2011Assignee: Novozymes A/SInventors: Allan Svendsen, Henriette Draborg
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Patent number: 8071345Abstract: The present invention relates to a liquid detergent composition comprising a surfactant, a subtilisin and a protease stabilizer that is 3-chlorobenzoic acid, 4-chlorobenzoic acid, 3-chlorophenylacetic acid, 3,5-dichlorobenzoic acid, 3-(3-chlorophenyl)propionic acid or their corresponding salts. The composition can further comprise a another enzyme that is a lipase, an amylase, a cellulase or mixtures thereof. The stabilizer can be present at a concentration of 0.001 to 20% w/w. The concentration of the subtilisin can be at least 1.5 g/L.Type: GrantFiled: March 29, 2007Date of Patent: December 6, 2011Assignee: Novozymes A/SInventors: Lone Kierstein Nielsen, Ole Simonsen, Karl Werntoft, Niclas Ilestam