Bacillus Subtilus Or Bacillus Lichenoformis Patents (Class 435/222)
  • Patent number: 10093911
    Abstract: The present invention relates to subtilisin variants and methods for obtaining subtilisin variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
    Type: Grant
    Filed: February 14, 2013
    Date of Patent: October 9, 2018
    Assignee: Novozymes A/S
    Inventors: Werner Besenmatter, Marco Malten, Benie Astrid
  • Patent number: 9357786
    Abstract: Polypeptide having activity inducing pathogen plant defense, nucleotide sequence encodes it, microorganism, compositions and methods. The polypeptide is a subtilisin derived from a strain of Acremonium strictum. The polypeptide is encoded by the nucleotide sequence SEQ ID NO: 1 or sequences at least 90% homologous to the same and has the amino acid sequence SEQ ID NO: 2 or sequences at least 90% homologous to the same.
    Type: Grant
    Filed: March 16, 2012
    Date of Patent: June 7, 2016
    Assignees: UNIVERSIDAD NACIONAL DE TUCUMAN, CONSEJO NACIONAL DE INVESTIGACIONES CIENTIFICAS Y TECNICAS (CONICET)
    Inventors: Atilio Pedro Castagnaro, Juan Carlos Diaz Ricci, Nadia Regina Chalfoun, Josefina Racedo, Sergio Miguel Salazar
  • Patent number: 9200239
    Abstract: Subtilase variants having an improved wash performance on egg stains. These subtilases are useful exhibiting excellent or improved wash performance on egg stains when used in e.g. cleaning or detergent compositions, such as laundry detergent compositions and dish wash compositions, including automatic dish wash compositions.
    Type: Grant
    Filed: June 5, 2014
    Date of Patent: December 1, 2015
    Assignee: Novozymes A/S
    Inventors: Stefan Minning, Jurgen Carsten Franz Knotzel, Niels Henrik Sorensen
  • Patent number: 9169475
    Abstract: The present invention relates to methods for producing variants of a parent TY145 subtilase and of a parent BPN? subtilase and to TY145 and BPN? variants having altered properties as compared to the parent TY145/BPN? subtilase.
    Type: Grant
    Filed: August 29, 2013
    Date of Patent: October 27, 2015
    Assignee: Novozymes A/S
    Inventors: Allan Svendsen, Henriette Draborg
  • Patent number: 9096838
    Abstract: The present invention provides engineered protease variants. In particular, the protease variants comprise combinable mutations at selected surface positions that affect the charge and/or hydrophobicity of the enzyme to enhance at least one desired property of the resulting variant enzyme in a chosen application. Compositions comprising the protease variants, and methods for using the same are also provided.
    Type: Grant
    Filed: May 2, 2014
    Date of Patent: August 4, 2015
    Assignee: Danisco US Inc.
    Inventors: Luis G. Cascao-Pereira, David A. Estell, James T. Kellis, Jr.
  • Patent number: 9062291
    Abstract: A transglutaminase protein which is mutated to have improved heat resistance and/or pH stability. A mutation is introduced into WT transglutaminase at a cysteine residue capable of forming a disulfide bond (SS bond).
    Type: Grant
    Filed: August 14, 2009
    Date of Patent: June 23, 2015
    Assignee: AJINOMOTO CO., INC.
    Inventors: Keiichi Yokoyama, Mototaka Suzuki, Tatsuki Kashiwagi, Eiichiro Suzuki, Masayo Date, Seiichi Taguchi
  • Publication number: 20150031589
    Abstract: The present invention provides methods for protein engineering and serine protease variants produced there from. Specifically, the present invention provides serine protease variants having one or more substitutions as compared to a reference serine protease. In addition, the present invention provides compositions comprising these serine protease variants. In some embodiments, the present invention provides cleaning compositions comprising at least one of these serine protease variants.
    Type: Application
    Filed: July 21, 2014
    Publication date: January 29, 2015
    Applicant: DANISCO US INC.
    Inventors: Wolfgang Aehle, Luis G. Cascao-Pereira, David A. Estell, Frits Goedegebuur, James T. Kellis, JR., Ayrookaran J. Poulose, Brian F. Schmidt
  • Publication number: 20140342433
    Abstract: The present invention relates to subtilase variants and methods for obtaining subtilase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
    Type: Application
    Filed: November 23, 2012
    Publication date: November 20, 2014
    Inventors: Allan Svendsen, Marco Malten, Christian Lundager Gylstorff
  • Patent number: 8889396
    Abstract: The present invention provides methods of improving growth performance, improving the efficiency of feed utilization, increasing feed digestibility, and decreasing mortality of immature and developing animals receiving animal feed. Methods of producing a crude keratinise enzyme extract and animal feed supplements for achieving the same are also provided.
    Type: Grant
    Filed: October 23, 2013
    Date of Patent: November 18, 2014
    Assignee: North Carolina State University
    Inventor: Jason C. H. Shih
  • Patent number: 8889395
    Abstract: A method for producing a crystalline and/or amorphous metabolite suspension from a cell fermentation broth in a continuous centrifuge process comprising (a) adding at a separate inlet to the centrifuge the fermentation broth comprising the cells and the metabolite of interest, wherein the metabolite is partly or wholly on crystalline and/or amorphous form; (b) adding at another inlet to the centrifuge an aqueous liquid comprising a salt and/or a carbohydrate having a higher density than the cells and a lower density than the metabolite of interest in its precipitated form; (c) removing the cells at a separate outlet to the centrifuge; and (d) removing the suspension comprising the crystalline and/or amorphous metabolite of interest at another outlet to the centrifuge.
    Type: Grant
    Filed: March 25, 2011
    Date of Patent: November 18, 2014
    Assignee: Novozymes A/S
    Inventors: Peter Frode Pind, Simon Glanville, Sune Jakobsen, Svend Kaasgaard
  • Publication number: 20140335596
    Abstract: The present invention relates to protease variants and methods for obtaining protease variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
    Type: Application
    Filed: December 18, 2012
    Publication date: November 13, 2014
    Inventors: Werner Besenmatter, Astrid Benie, Esben Peter Friis, Pernille Ollendorf Micheelsen
  • Patent number: 8883444
    Abstract: The invention relates to a method for synthesizing a peptide by enzymatically preparing an ester or thioester from (i) an N-terminal protected amino acid or an N-terminal protected peptide where either can have a protected C-terminal ester group and (ii) an alcohol represented by the formula HO—CX2—Z or a thiol represented by the formula HS—CX2—Z, each X independently representing a halogen atom or a hydrogen atom; and Z represents an electron withdrawing group comprising at least one sp3-hybridized carbon comprising at least two substituents comprising a heteroatom directly attached to the at least one sp3-hybridized carbon or at least one sp2-hybridized carbon comprising one or two substituents comprising a heteroatom directly attached to the at least one sp2-hybridized carbon, and enzymatically coupling the prepared ester or thioester with an optionally C-terminal protected amino acid or with an optionally C-terminal protected peptide in a medium comprising 2 wt. % water or less.
    Type: Grant
    Filed: November 19, 2009
    Date of Patent: November 11, 2014
    Assignee: Enzypep B.V.
    Inventors: Peter Jan Leonard Mario Quaedflieg, Timo Nuijens, Claudia Cusan, Catharina Hubertina Maria Schepers
  • Publication number: 20140308736
    Abstract: The present invention provides engineered protease variants. In particular, the protease variants comprise combinable mutations at selected surface positions that affect the charge and/or hydrophobicity of the enzyme to enhance at least one desired property of the resulting variant enzyme in a chosen application. Compositions comprising the protease variants, and methods for using the same are also provided.
    Type: Application
    Filed: May 2, 2014
    Publication date: October 16, 2014
    Applicant: DANISCO US INC.
    Inventors: Luis G. Cascao-Pereira, David A. Estell, James T. Kellis, JR.
  • Publication number: 20140302590
    Abstract: The present invention provides protease compositions particularly suited for dishwashing applications.
    Type: Application
    Filed: April 7, 2014
    Publication date: October 9, 2014
    Applicant: Danisco US Inc.
    Inventors: Pieter Augustinus, Frits Goedegebuur, Ayrookaran J. Poulose, Johannes C. Van Der Laan
  • Publication number: 20140294881
    Abstract: The present invention provides novel protein variants that exhibit reduced immunogenic responses, as compared to the parental proteins. The present invention further provides DNA molecules that encode novel variants, host cells comprising DNA encoding novel variants, as well as methods for making proteins less allergenic. In addition, the present invention provides various compositions that comprise these proteins that are less immunogenic than the wild-type proteins.
    Type: Application
    Filed: September 18, 2008
    Publication date: October 2, 2014
    Applicant: Danisco US Inc.
    Inventors: David A. Estell, Fiona A. Harding, Ayrookaran J. Poulose
  • Publication number: 20140295527
    Abstract: The present invention provides a Bacillus sp. subtilisin variant. In addition, the present invention provides automatic dishwashing compositions comprising this serine protease variant.
    Type: Application
    Filed: April 22, 2014
    Publication date: October 2, 2014
    Applicant: DANISCO US INC.
    Inventors: David A. Estell, Frits Goedegebuur, Ayrookaran J. Poulose
  • Patent number: 8802416
    Abstract: The present invention relates to a thermostable protease useful as an enzyme for industrial use, a gene encoding the same and a method of producing the enzyme by genetic engineering technique. More particularly, the present invention discloses a thermostable organic solvent tolerant protease and its code gene and application. The invention also discloses a method for preparing protease by isolating from Bacillus subtilis isolate Rand bacteria. The activity and stability of protease (preferably named Rand protease) at high temperature, and can be used in fields of washing agent industry, foodstuff industry, biological pharmacy and environmental biological technique.
    Type: Grant
    Filed: October 7, 2010
    Date of Patent: August 12, 2014
    Assignee: Universiti Putra Malaysia
    Inventors: Raja Noor Zaliha Raja Abd Rahman, Abu Bakar Salleh, Mahiran Basri, Randa Abdelkareem Abusham
  • Publication number: 20140212385
    Abstract: Polypeptide having activity inducing pathogen plant defence, nucleotide sequence encodes it, microorganism, compositions and methods. The polypeptide is a subtilisin derived from a strain of Acremonium strictum. The polypeptide is encoded by the nucleotide sequence SEQ ID No.: 1 or sequences at least 90% homologous to the same and has the amino acid sequence SEQ ID No: 2 or sequences at least 90% homologous to the same.
    Type: Application
    Filed: March 16, 2012
    Publication date: July 31, 2014
    Applicant: CONSEJO NACIONAL DE INVESTIGACIONES CIENTIFICAS Y TECNICAS (CONICET)
    Inventors: Atilio Pedro Castagnaro, Juan Carlos Diaz Ricci, Nadia Regina Chalfoun, Josefina Racedo, Sergio Miguel Salazar
  • Publication number: 20140199752
    Abstract: A method of producing an enzyme of interest in a fed-batch cultivation comprising: a) cultivating a microorganism in a culture medium conducive to its growth wherein the microorganism produces the enzyme of interest; and b) adding a manganese compound to the culture medium one or more times during the cultivation.
    Type: Application
    Filed: August 3, 2012
    Publication date: July 17, 2014
    Applicant: NOVOZYMES A/S
    Inventors: Jon Martin Persson, Niels Banke
  • Patent number: 8753861
    Abstract: The present invention provides engineered protease variants. In particular, the protease variants comprise combinable mutations at selected surface positions that affect the charge and/or hydrophobicity of the enzyme to enhance at least one desired property of the resulting variant enzyme in a chosen application. Compositions comprising the protease variants, and methods for using the same are also provided.
    Type: Grant
    Filed: November 10, 2009
    Date of Patent: June 17, 2014
    Assignee: Danisco US Inc.
    Inventors: Luis G. Cascao-Pereira, David A. Estell, James T. Kellis, Jr.
  • Publication number: 20140073552
    Abstract: The present invention relates to enzymes produced by mutating the genes for a number of subtilases and expressing the mutated genes in suitable hosts are presented. The enzymes exhibit improved stability and/or improved wash performance in any detergent in comparison to their wild type parent enzymes. The enzymes are well-suited for use in any detergent and for some in especially liquid or solid shaped detergent compositions.
    Type: Application
    Filed: October 1, 2012
    Publication date: March 13, 2014
    Applicants: UNILEVER PLC, NOVOZYMES A/S
    Inventors: Laurens Nicolaas Sierkstra, Jan Klugkist, Peter Markvardsen, Claus Von Der Osten, Peter Bauditz
  • Patent number: 8642313
    Abstract: The present invention provides methods of improving growth performance, improving the efficiency of feed utilization, increasing feed digestibility, and decreasing mortality of immature and developing animals receiving animal feed. Methods of producing a crude keratinase enzyme extract and animal feed supplements for achieving the same are also provided.
    Type: Grant
    Filed: February 25, 2011
    Date of Patent: February 4, 2014
    Assignee: North Carolina State University
    Inventor: Jason C. H. Shih
  • Publication number: 20140011234
    Abstract: The present invention provides cells that have been genetically manipulated to have an altered capacity to produce expressed proteins. In particular, the present invention relates to Gram-positive microorganisms, such as Bacillus species having enhanced expression of a protein of interest, wherein one or more chromosomal genes have been inactivated, and preferably wherein one or more chromosomal genes have been deleted from the Bacillus chromosome. In some further embodiments, one or more indigenous chromosomal regions have been deleted from a corresponding wild-type Bacillus host chromosome.
    Type: Application
    Filed: January 18, 2013
    Publication date: January 9, 2014
    Applicant: DANISCO US INC.
    Inventors: Eugenio Ferrari, Carole Harbison, M. Harunur Rashid, Walter Weyler
  • Patent number: 8623630
    Abstract: The present invention provides methods of altering the production of desired polypeptides in a host cell. In particular, the present invention provides polynucleotides encoding truncated SecG proteins capable of facilitating the secretion of desired proteases by a bacterial host cell, such as Bacillus species, as well as expression vectors and a host cell containing the polynucleotides.
    Type: Grant
    Filed: October 15, 2012
    Date of Patent: January 7, 2014
    Assignee: Danisco US Inc.
    Inventors: Anita Van Kimmenade, Carol Fioresi, Caroline Peres, Eugenio Ferrari
  • Publication number: 20130344568
    Abstract: The present invention relates to methods for producing variants of a parent TY145 subtilase and of a parent BPN? subtilase and to TY145 and BPN? variants having altered properties as compared to the parent TY145/BPN? subtilase.
    Type: Application
    Filed: August 29, 2013
    Publication date: December 26, 2013
    Applicant: Novozymes A/S
    Inventors: Allan Svendsen, Henriette Draborg
  • Publication number: 20130323816
    Abstract: The present invention provides protease compositions particularly suited for dishwashing applications.
    Type: Application
    Filed: April 25, 2013
    Publication date: December 5, 2013
    Applicant: DANISCO US INC.
    Inventors: Pieter Augustinus, Frits Goedegebuur, Ayrookaran J. Poulose, Johannes C. Van Der Laan
  • Patent number: 8524223
    Abstract: A microorganism inhibits the growth of various plant pathogens, and is not reduced in its efficacy even when the microorganism is used in combination with a chemical pesticide. The microorganism is Bacillus subtilis KS1 strain (NITE BP-569). The plant disease control agent comprises a culture of the microorganism as an active ingredient.
    Type: Grant
    Filed: July 3, 2009
    Date of Patent: September 3, 2013
    Assignee: University of Yamanashi
    Inventors: Tsutomu Takayanagi, Shunji Suzuki, Seiichi Furuya
  • Publication number: 20130216519
    Abstract: The present invention provides novel protein variants that exhibit reduced immunogenic responses, as compared to the parental proteins. The present invention further provides DNA molecules that encode novel variants, host cells comprising DNA encoding novel variants, as well as methods for making proteins less allergenic. In addition, the present invention provides various compositions that comprise these proteins that are less immunogenic than the wild-type proteins.
    Type: Application
    Filed: February 1, 2013
    Publication date: August 22, 2013
    Applicant: DANISCO US INC.
    Inventor: DANISCO US INC.
  • Publication number: 20130164818
    Abstract: Biomass (e.g., plant biomass, animal biomass, microbial, and municipal waste biomass) is processed to produce useful products, such as food products and amino acids.
    Type: Application
    Filed: December 6, 2011
    Publication date: June 27, 2013
    Applicant: XYLECO, INC.
    Inventor: Marshall Medoff
  • Publication number: 20130122571
    Abstract: The present invention relates to methods for producing variants of a parent TY145 subtilase and of a parent BPN? subtilase and to TY145 and BPN? variants having altered properties as compared to the parent TY145/BPN? subtilase.
    Type: Application
    Filed: January 17, 2013
    Publication date: May 16, 2013
    Applicant: NOVOZYMES A/S
    Inventor: Novozymes A/S
  • Publication number: 20130102052
    Abstract: The present invention provides methods of altering the production of desired polypeptides in a host cell. In particular, the present invention provides polynucleotides encoding truncated SecG proteins capable of facilitating the secretion of desired proteases by a bacterial host cell, such as Bacillus species, as well as expression vectors and a host cell containing the polynucleotides.
    Type: Application
    Filed: October 15, 2012
    Publication date: April 25, 2013
    Applicant: DANISCO US INC.
    Inventor: DANISCO US INC.
  • Publication number: 20130065223
    Abstract: The present invention provides a universally applicable lysis buffer comprising a chaotropic 5 agent, a reducing agent, and a proteolytic enzyme suitable for processing a wide variety o different sample types, such as different types of bodily samples relevant for the diagnosis o a respiratory disease. Furthermore, the present invention provides the use of a chaotropic agent, a reducing agent, and a proteolytic enzyme for the lysis of a broad spectrum of bodily samples. Moreover, the present invention provides a method for processing bodily samples which is universally applicable to the lysis of a variety of different types of bodily samples. Furthermore, the present invention provides methods for analyzing a bodily sample or for detecting the presence of a pathogen in a bodily sample, preferably, for diagnosing a respiratory disease, such as pneumonia or tuberculosis.
    Type: Application
    Filed: May 9, 2011
    Publication date: March 14, 2013
    Applicant: CURETIS AG
    Inventors: Matthias Klein, Gerd Lüdke, Andreas Boos
  • Publication number: 20130029402
    Abstract: The present invention provides host cells that have been genetically manipulated to have an enhanced capacity to produce proteins of interest. In particular, the invention relates to modified Bacillus sp. Host cells that have at least one inactivated phr gene. The enhanced production of proteins of interest by the modified Bacillus sp. Host cells is further increased in modified Bacillus sp. Host cells that overexpress YmaH.
    Type: Application
    Filed: September 19, 2012
    Publication date: January 31, 2013
    Applicant: DANISCO US INC.
    Inventor: Danisco US Inc.
  • Publication number: 20130017592
    Abstract: A method for producing a crystalline and/or amorphous metabolite suspension from a cell fermentation broth in a continuous centrifuge process comprising (a) adding at a separate inlet to the centrifuge the fermentation broth comprising the cells and the metabolite of interest, wherein the metabolite is partly or wholly on crystalline and/or amorphous form; (b) adding at another inlet to the centrifuge an aqueous liquid comprising a salt and/or a carbohydrate having a higher density than the cells and a lower density than the metabolite of interest in its precipitated form; (c) removing the cells at a separate outlet to the centrifuge; and (d) removing the suspension comprising the crystalline and/or amorphous metabolite of interest at another outlet to the centrifuge.
    Type: Application
    Filed: March 25, 2011
    Publication date: January 17, 2013
    Applicant: NOVOZYMES A/S
    Inventors: Peter Frode Pind, Simon Glanville, Sune Jakobsen, Svend Kaasgaard
  • Publication number: 20130005637
    Abstract: Proteases encompassing an amino acid sequence that is at least 70% identical to the amino acid sequence indicated in SEQ ID NO. 1 over its entire length, and exhibit the amino acid substitution I21V in the count in accordance with SEQ ID NO. 1, resp. agents that encompass such proteases, display very good cleaning performance on protease-sensitive stains.
    Type: Application
    Filed: March 10, 2011
    Publication date: January 3, 2013
    Applicant: HENKEL AG & CO. KGAA
    Inventors: Petra Siegert, Ulrich Schwaneberg, Ronny Martinez, Marion Merkel, Astrid Spitz, Susanne Wieland, Hendrik Hellmuth, Karl-Heinz Maurer
  • Publication number: 20120295331
    Abstract: Subtilase variants having an improved wash performance on egg stains. These subtilases are useful exhibiting excellent or improved wash performance on egg stains when used in e.g. cleaning or detergent compositions, such as laundry detergent compositions and dish wash compositions, including automatic dish wash compositions.
    Type: Application
    Filed: July 23, 2012
    Publication date: November 22, 2012
    Applicant: NOVOZYMES A/S
    Inventors: Stefan Minning, Jürgen Carsten Franz Knötzel, Niels Henrik Sørensen
  • Patent number: 8241885
    Abstract: The present invention is directed to the identification of a protease prodomain that is capable of binding a corresponding protease with high affinity. The protease prodomain of the present invention is fused to a second protein to form a protease prodomain fusion protein. The presence of a protease prodomain protein in a fusion protein allows for easy and selective purification of the second protein by incubation with the corresponding protease.
    Type: Grant
    Filed: September 22, 2010
    Date of Patent: August 14, 2012
    Assignee: University of Maryland, College Park
    Inventor: Philip N. Bryan
  • Publication number: 20120165241
    Abstract: The present invention relates to enzymes produced by mutating the genes for a number of subtilases and expressing the mutated genes in suitable hosts are presented. The enzymes exhibit improved stability and/or improved wash performance in any detergent in comparison to their wild type parent enzymes. The enzymes are well-suited for use in any detergent and for some in especially liquid or solid shaped detergent compositions.
    Type: Application
    Filed: March 7, 2012
    Publication date: June 28, 2012
    Applicants: UNILEVER PLC, NOVOZYMES A/S
    Inventors: Laurens Nicolaas Sierkstra, Jan Klugkist, Peter Markvardsen, Claus von der Osten, Peter Bauditz
  • Publication number: 20120156721
    Abstract: This invention provides novel chemically modified mutant serine hydrolases that catalyze a transamidation and/or a transpeptidation and/or a transesterification reaction. The modified serine hydrolases have one or more amino acid residues in a subsite replaced with a cysteine, wherein the cysteine is modified by replacing the thiol hydrogen in the cysteine with a substituent group providing a thiol side chain comprising a moiety selected from the group consisting of a polar aromatic substituent, an alkyl amino group with a positive charge, and a glycoside. In particularly preferred embodiments, the substitutents include an oxazolidinone, a C1 to C15 alkyl amino group with a positive charge, or a glycoside.
    Type: Application
    Filed: February 24, 2012
    Publication date: June 21, 2012
    Applicant: The Governing Council of the University of Toronto
    Inventors: John Bryan Jones, Michael Dickman, Richard C. Lloyd
  • Publication number: 20120142076
    Abstract: The present invention provides host cells that have been genetically manipulated to have an enhanced capacity to produce proteins of interest. In particular, the invention relates to modified Bacillus sp. Host cells that have at least one inactivated phr gene. The enhanced production of proteins of interest by the modified Bacillus sp. Host cells is further increased in modified Bacillus sp. Host cells that overexpress YmaH.
    Type: Application
    Filed: June 2, 2010
    Publication date: June 7, 2012
    Applicant: Danisco US Inc.
    Inventors: Cristina Bongiorni, Eugenio Ferrari
  • Publication number: 20120094358
    Abstract: Biomass (e.g., plant biomass, animal biomass, microbial, and municipal waste biomass) is processed to produce useful products, such as food products and amino acids.
    Type: Application
    Filed: December 6, 2011
    Publication date: April 19, 2012
    Applicant: XYLECO, INC.
    Inventor: Marshall Medoff
  • Patent number: 8101563
    Abstract: The present invention relates to the identification of novel metallo-proteases (MP) in Gram-positive microorganisms. The present invention provides the nucleic acid and amino acid sequences for Bacillus MP. The present invention also provides host cells having a mutation or deletion of part or all of the gene encoding MP. The present invention also provides host cells further comprising nucleic acid encoding desired heterologous proteins such as enzymes. The present invention also provides cleaning compositions comprising an MP of the present invention.
    Type: Grant
    Filed: December 8, 2004
    Date of Patent: January 24, 2012
    Assignee: Danisco US Inc.
    Inventor: David A. Estell
  • Publication number: 20120009651
    Abstract: The present invention provides methods and compositions comprising at least one thermolysin-like neutral protease enzyme with improved storage stability and/or catalytic activity. In some embodiments, the thermolysin finds use in cleaning and other applications comprising detergent. In some particularly preferred embodiments, the present invention provides methods and compositions comprising thermolysin formulated and/or engineered to resist detergent-induced inactivation.
    Type: Application
    Filed: October 28, 2008
    Publication date: January 12, 2012
    Applicant: DANISCO US INC.
    Inventors: David A. Estell, Ronaldus W.J. Hommes, Amy D. Liu, Andrew Shaw
  • Patent number: 8084241
    Abstract: The present invention relates to methods for producing variants of a parent TY145 subtilase and of a parent BPN? subtilase and to TY145 and BPN? variants having altered properties as compared to the parent TY145/BPN? subtilase.
    Type: Grant
    Filed: September 10, 2010
    Date of Patent: December 27, 2011
    Assignee: Novozymes A/S
    Inventors: Allan Svendsen, Henriette Draborg
  • Patent number: 8071345
    Abstract: The present invention relates to a liquid detergent composition comprising a surfactant, a subtilisin and a protease stabilizer that is 3-chlorobenzoic acid, 4-chlorobenzoic acid, 3-chlorophenylacetic acid, 3,5-dichlorobenzoic acid, 3-(3-chlorophenyl)propionic acid or their corresponding salts. The composition can further comprise a another enzyme that is a lipase, an amylase, a cellulase or mixtures thereof. The stabilizer can be present at a concentration of 0.001 to 20% w/w. The concentration of the subtilisin can be at least 1.5 g/L.
    Type: Grant
    Filed: March 29, 2007
    Date of Patent: December 6, 2011
    Assignee: Novozymes A/S
    Inventors: Lone Kierstein Nielsen, Ole Simonsen, Karl Werntoft, Niclas Ilestam
  • Publication number: 20110256610
    Abstract: The present invention provides variant subtilisins and compositions comprising at least one variant subtilisin set forth herein, as well as methods for using these variants and compositions. In some embodiments, the present invention provides variant proteases suitable for laundry cleaning applications.
    Type: Application
    Filed: June 3, 2009
    Publication date: October 20, 2011
    Inventors: Viktor Yuryevich Alekseyev, Luis G. Cascao-Pereira, James T. Kellis, JR., Alexander Pisarchik
  • Patent number: 7972835
    Abstract: A blood-viscosity reducing agent contains a protein derived from Bacillus subtilis natto and including, sequentially from an amino group terminal, a first structural amino acid sequence having alanine, threonine, aspartic acid, glycine, valine, glutamic acid, tryptophan, asparagine, valine, aspartic acid, glutamine, isoleucine, aspartic acid, alanine, proline, lysine, alanine, tryptophan, alanine, leucine, glycine, tyrosine aspartic, acid, glycine, threonine, glycine, threonine, valine, valine, alanine, serine, isoleucine, aspartic acid, threonine, glycine, valine, glutamic acid, tryptophan, asparagine, histidine, proline, alanine, leucine, lysine, glutamic acid, lysine, tyrosine, arginine, glycine, tyrosine, asparagine, proline, glutamic acid, asparagine, proline, asparagine, glutamic acid, proline, glutamic acid, asparagine, glutamic acid, methionine, asparagine, tryptophan, tyrosine, aspartic acid, alanine, valine, alanine, glycine, glutamic acid, alanine, serine, proline, tyrosine, aspartic acid, aspartic
    Type: Grant
    Filed: February 11, 2009
    Date of Patent: July 5, 2011
    Assignees: Daiwa Pharmaceutical Co., Ltd.
    Inventors: Masahito Hitosugi, Hiroaki Maeda, Kazunobu Omura
  • Publication number: 20110117624
    Abstract: The present invention relates to isolated proteases of the RP-II type and variants of RP-II proteases exhibiting improved properties in comparison to the parent RP-II protease, DNA constructs and vectors coding for the expression of said proteases and variants, host cells capable of expressing the proteases and variants from the DNA constructs, as well as a method of producing them by cultivating said host cells. The proteases may advantageously be used as constituents in detergent compositions and additives, optionally in combination with other enzymes such as proteases, lipases, cellulases, amylases, peroxidases or oxidases.
    Type: Application
    Filed: January 26, 2011
    Publication date: May 19, 2011
    Applicant: Novozymes A/S
    Inventors: Mads Norregaard-Madsen, Peter Rabbek Østergaard, Claus Bo Voge Christensen, Søren Flensted Lassen
  • Publication number: 20110110910
    Abstract: The pharmaceutical use of proteases related to amino acids 1-274 of SEQ ID NO: 2, the serine protease derived from Bacillus licheniformis, which is also designated subtilisin Carlsberg, optionally in combination with a lipase and/or an amylase. Examples of medical indications are: Treatment of digestive disorders, pancreatic exocrine insufficiency (PEI), pancreatitis, cystic fibrosis, diabetes type I, and/or diabetes type II.
    Type: Application
    Filed: January 19, 2011
    Publication date: May 12, 2011
    Applicants: Novozymes A/S, Solvay Pharmaceuticals GmbH
    Inventors: Allan Svendsen, Claus Crone Fuglsang, Peter Colin Gregory
  • Publication number: 20110097784
    Abstract: The present invention relates to a thermostable protease useful as an enzyme for industrial use, a gene encoding the same and a method of producing the enzyme by genetic engineering technique. More particularly, the present invention discloses a thermostable organic solvent tolerant protease and its code gene and application. The invention also discloses a method for preparing protease by isolating from Bacillus subtilis isolate Rand bacteria. The activity and stability of protease (preferably named Rand protease) at high temperature, and can be used in fields of washing agent industry, foodstuff industry, biological pharmacy and environmental biological technique.
    Type: Application
    Filed: October 7, 2010
    Publication date: April 28, 2011
    Inventors: Raja Noor Zaliha Raja Abd Rahman, Abu Bakar Salleh, Mahiran Basri, Randa Abdelkareem Abusham