Derived From Bacteria Patents (Class 435/220)
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Publication number: 20090042272Abstract: The invention provides novel methionine aminopeptidase enzymes and their use.Type: ApplicationFiled: July 22, 2008Publication date: February 12, 2009Applicant: Novo Nordisk A/SInventors: Inga Sig Nielsen Norby, Lars Fogh Iversen
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Patent number: 7485447Abstract: A secreted proteolytic polypeptide comprising at least three non-polar or uncharged polar amino acids within the last four amino acids of the C-terminus of the polypeptide, encoding polynucleotides, expression vectors comprising the polynucleotides, host cell comprising the polynucleotides, methods for producing said polypeptide, and methods for using the polypeptide.Type: GrantFiled: June 21, 2004Date of Patent: February 3, 2009Assignee: Novozymes A/SInventor: Soren Flensted Lassen
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Publication number: 20080292704Abstract: The present invention concerns inventive polypeptides. The present invention also concerns compositions and vaccines comprising the inventive polypeptides. In other embodiments of the invention, the inventive polypeptides are provided to a subject, used to vaccinate, or used to induce immunity. Other embodiments include methods for making the inventive polypeptides and nucleic acids used to encode the inventive polypeptides.Type: ApplicationFiled: March 6, 2008Publication date: November 27, 2008Applicant: ITERATIVE THERAPEUTICS, INC.Inventors: Barry G.W. Arnason, Mark A. Jensen, David M. White
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Patent number: 7452701Abstract: Purified ?-amino acids are of considerable interest in the preparation of pharmacologically active compounds and industrial precursors. Although enantiomerically pure ?-amino acids can be produced by standard chemical synthesis, this traditional approach is time consuming, requires expensive starting materials, and results in a racemic mixture which must be purified further. However, DNA molecules encoding lysine 2,3-aminomutase can be used to prepare ?-amino acids by methods that avoid the pitfalls of chemical synthesis. The present invention provides a method of producing enantiomerically pure ?-amino acids from ?-amino acids comprising catalyzing the conversion of an ?-amino acid to a corresponding ?-amino acid by utilizing a lysine 2,3-aminomutase as the catalyst.Type: GrantFiled: September 5, 2002Date of Patent: November 18, 2008Assignee: Wisconsin Alumni Research FoundationInventors: Perry A. Frey, Frank J. Ruzicka
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Patent number: 7449305Abstract: The present invention provides a method of pretreating a sample containing a glycated amine as an analyte, thereby enabling highly reliable measurement of a glycated amine. A glycated amino acid in the sample is degraded by causing a fructosyl amino acid oxidase (FAOD) to act thereon, and thereafter, a FAOD further is caused to act on the glycated amine as the analyte in the sample to cause a redox reaction. The amount of the glycated amine is determined by measuring the redox reaction. The substrate specificity of the FAOD caused to act on the glycated amino acid may be either the same as or different from that of the FAOD caused to act on the glycated amine. When using the same FAOD, a FAOD is caused to act on the glycated amino acid to degrade it, and thereafter, the sample is treated with a protease to inactivate the FAOD and also to degrade the glycated amine.Type: GrantFiled: October 9, 2002Date of Patent: November 11, 2008Assignee: ARKRAY, Inc.Inventors: Satoshi Yonehara, Tsuguki Komori
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Publication number: 20080227175Abstract: A method of solubilizing protease crystals and/or protease precipitate in a fermentation broth comprising a) diluting the fermentation broth 100-2000% (w/w); b) adding a divalent salt; and c) adjusting the pH value of the fermentation broth to a pH value below pH 5.5.Type: ApplicationFiled: March 7, 2008Publication date: September 18, 2008Applicant: Novozymes A/SInventors: Sune Jakobsen, Kim Uhre Hansen, Svend Kaasgaard
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Publication number: 20080220499Abstract: The present invention relates to an extracellular enzyme protease obtained by growing the culture of Pseudomonas aeruginosa MCM B-327 isolated from vermiculture pit soil and deposited in MTCC, IMTECH, Chandigarh with designation MTCC 5270, in production medium of pH 7.0; containing soybean meal and tryptone as raw materials, at 30° C. for 72 h. The organism was also able to produce protease using different agricultural products/byproducts as protein sources. The partially purified non-collagenolytic, calcium independent protease with molecular weight 60 kDa has activity in pH range of 6.0-11.0 and temperature range of 25-65° C.; stability in pH range of 6.0-10.0 and temperature 25-45° C. The protease activity was retained for 8 months when stored at ambient temperature. Ammonium sulphate precipitated enzyme was able to completely dehair animal skins and hides without chemicals like lime, sodium sulphide and calcium.Type: ApplicationFiled: October 31, 2007Publication date: September 11, 2008Inventors: Smita Shrikant Nilegaonkar, Vasudeo Pandharinath Zambare, Pradnya Pralhad Kanekar, Prashant Kamalakar Dhakephalkar, Seema Shreepad Sarnaik, Narasimhan Kannan Chandra Babu, Baddipudi Ramaniah, Rama Rajaram, Thirumalachari Ramasami, Yegnisettipalli Krishnaiah Saikumari, Padmanabhan Balaram
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Publication number: 20080221012Abstract: Compositions comprising activatable recombinant neurotoxins and polypeptides derived therefrom. The invention also comprises nucleic acids encoding such polypeptides, and methods of making such polypeptides and nucleic acids.Type: ApplicationFiled: August 27, 2007Publication date: September 11, 2008Applicant: Allergan, Inc.Inventors: Lance E. Steward, Joseph Francis, Ester Fernandez-Salas, Marcella A. Gilmore, Shengwen Li, J. Oliver Dolly, Kei Roger Aoki
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Patent number: 7410638Abstract: The present invention relates to isolated nucleic acid molecules encoding an autoinducer inactivation protein, wherein the encoded protein comprises an amino acid sequence selected from the group consisting of 104HXHXDH109˜60aa˜H169˜21aa˜D191 and 103HXHXDH108˜72aa˜H180˜21aa˜D202, and to expression vectors and transformed plant and animal cells comprising the same. The proteins encoded by these nucleic acid molecules provide to a susceptible plant or animal increased resistance to a disease the virulence of which is regulated by autoinducers. Also provided are methods of increasing disease resistance in susceptible plants and animals.Type: GrantFiled: August 23, 2000Date of Patent: August 12, 2008Assignee: Institute of Molecular AgrobiologyInventors: Lianhui Zhang, Yihu Dong, Haibao Zhang, Jinling Xu
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Patent number: 7407789Abstract: The present invention relates to the biotechnology field, more particularly, to novel recombinant staphylokinase (RGD/KGD-Sak) derivatives and the preparation the thereof. The derivatives, have a low polymerizing ability, low immunogenicity and a bifunctionality of thrombolytics and anticoagulant. Based on the trimensional structural analysis of the monomer and dimer of recombinant staphylokinases and their biochemical properties, we designed two novel bifunctional staphylokinase molecular structures. Mutant genes were constructed by PCR site-directed mutagenesis, which were then recombined with a prokaryotic vector and used to transform E. coli. Engineered strains with a high expression level were selected by screening and propagated by fermentation, followed by disruption of the cells, centrifugation to collect inclusion bodies, renaturation, and purification of RGD/KGD-SAK through a two-step method. After lyophilized, the polymerizing ability and immunogenicity of the products decreased significantly.Type: GrantFiled: January 23, 2001Date of Patent: August 5, 2008Assignee: Fudan UniversityInventors: Houyan Song, Gang Song
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Publication number: 20080178306Abstract: A transmissible spongiform encephalopathy (TSE) agent is inactivated by exposing the TSE agent to a thermostable proteolytic enzyme at elevated temperature and at acid or alkaline pH. Following this step, or separately, presence of TSE infectivity is detected by detection of dimers of prion protein.Type: ApplicationFiled: October 11, 2007Publication date: July 24, 2008Inventors: Neil David Hammond Raven, John Mark Sutton
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Patent number: 7354745Abstract: Purified ?-amino acids are of considerable interest in the preparation of pharmacologically active compounds. Although enantiomerically pure ?-amino acids, such as L-?-lysine, can be produced by standard chemical synthesis, this traditional approach is time consuming, requires expensive starting materials, and results in a racemic mixture which must be purified further. However, DNA molecules encoding lysine 2,3-aminomutase can be used to prepare L-?-lysine by methods that avoid the pitfalls of chemical synthesis. In particular, L-?-lysine can be synthesized by cultures of host cells that express recombinant lysine 2,3-aminomutase. Alternatively, such recombinant host cells can provide a source for isolating quantities of lysine 2,3-aminomutase, which in turn, can be used to produce L-?-lysine in vitro.Type: GrantFiled: May 1, 2001Date of Patent: April 8, 2008Assignee: Wisconsin Alumni Research FoundationInventors: Perry A. Frey, Frank J. Ruzicka
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Patent number: 7335486Abstract: The invention provides methods for making peptides from a polypeptide containing at least one copy of the peptide using clostripain to excise the peptide from the polypeptide. The methods enable the use of a single, highly efficient enzymatic cleavage to produce any desired peptide sequence.Type: GrantFiled: November 24, 2004Date of Patent: February 26, 2008Assignee: Restoragen, Inc.Inventors: Fred W. Wagner, Peng Luan, Yuannan Xia, Daniel Strydom, Edwin H. Merrifield, Mary J. Bossard, Barton Holmquist, Jin Seog Seo
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Patent number: 7332320Abstract: The present invention provides novel protein variants that exhibit reduced immunogenic responses, as compared to the parental proteins. The present invention further provides DNA molecules that encode novel variants, host cells comprising DNA encoding novel variants, as well as methods for making proteins less allergenic. In addition, the present invention provides various compositions that comprise these proteins that are less immunogenic than the wild-type proteins.Type: GrantFiled: December 20, 2002Date of Patent: February 19, 2008Assignee: Genencor International, Inc.Inventors: David A Estell, Fiona A. Harding
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Patent number: 7326531Abstract: The present invention relates to the identification of novel metallo-proteases (MP) in Gram-positive microorganisms. The present invention provides the nucleic acid and amino acid sequences for Bacillus MP. The present invention also provides host cells having a mutation or deletion of part or all of the gene encoding MP. The present invention also provides host cells further comprising nucleic acid encoding desired heterologous proteins such as enzymes. The present invention also provides cleaning compositions comprising an MP of the present invention.Type: GrantFiled: December 8, 2004Date of Patent: February 5, 2008Assignee: Genencor International, Inc.Inventor: David A. Estell
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Patent number: 7306937Abstract: Novel enzyme variants including protease variants derived from the DNA sequences of naturally-occurring or re-combinant non-human proteases are disclosed. The variant proteases, in general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant protease to generate the substitution of a plurality of amino acid residues in the amino acid sequence of a precursor protease. Such variant proteases have properties which are different from those of the precursor protease, such as altered wash performance. The substituted amino acid residue correspond to positions 27, 45, 170, 181, 251 and 271 of Bacillus amyloliquefaciens subtilisin. Additional variants comprising at least one additional substitution at a position selected from 1, 14, 49, 61, 87, 100, 102, 118, 128, 204 and 258 of Bacillus amyloliquefaciens subtilisin are also described.Type: GrantFiled: January 16, 2003Date of Patent: December 11, 2007Assignee: Genencor International, Inc.Inventors: Ayrookaran J. Poulose, David A Estell, James T Kellis, Jr., Richard R. Bott
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Patent number: 7297332Abstract: The present invention relates to a protease, and more specifically to a protease derived from Aranicola proteolyticus, a gene coding for said enzyme, a gene expression system for said protease, a process for purifying the protease, and the uses of said protease in industrial applications, such as for example, detergents, cosmetics, leather processing agents, chemicals for laboratory research, solubilizing or softening agents for food, meat modifier, feed or food additives, or oil and fat separating agents, as well as pharmaceutical compositions.Type: GrantFiled: December 29, 2000Date of Patent: November 20, 2007Assignee: Korean Research Institute of Bioscience and BiotechnologyInventors: Ho-Yong Park, Kwang-Hee Son, Doo-Sang Park, Sang-Woon Shin, Hyun-Woo Oh, Mi-Gwang Kim, Dong-Ha Shin
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Patent number: 7297528Abstract: An alkaline protease having the following properties; a gene encoding the same; a microorganism producing the same; and washing compositions containing the same; (i) acting over a broad pH value range of 4 to 13 and achieving, at pH 6 to 12, 80% or more the activity at the optimum pH value; (ii) when treated at 40° C. for 30 minutes, being stable over a pH value range of 6 to 11; (iii) having an isoelectric point of about 8.9 to 9.1; and (iv) having casein digesting activity that is not inhibited by oleic acid. The alkaline protease of the present invention is highly stable to various surface active agents and fatty acids, and exhibits high stability to oxidizing agents, and is therefore useful as an enzyme to be used in detergents for automatic dishwashers and laundry detergents, both containing bleaching components.Type: GrantFiled: February 24, 2004Date of Patent: November 20, 2007Assignee: Kao CorporationInventors: Mikio Takaiwa, Mitsuyoshi Okuda, Katsuhisa Saeki, Hiromi Kubota, Jun Hitomi, Yasushi Kageyama, Shitsuw Shikata, Masafumi Nomura
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Patent number: 7288389Abstract: DNA and recombinant DNA that encode a peptide-forming enzyme, a method for producing a peptide-forming enzyme, and a method for producing a dipeptide are disclosed. A method for producing a dipeptide includes producing a dipeptide from a carboxy component and an amine component by using a culture of a microbe belonging to the genus Sphingobacterium and having the ability to form the dipeptide from the carboxy component and the amine component, a microbial cell separated from the culture, treated microbial cell product of the microbe or a peptide-forming enzyme derived from the microbe.Type: GrantFiled: May 28, 2004Date of Patent: October 30, 2007Assignee: Ajinomoto Co., Inc.Inventors: Seiichi Hara, Kenzo Yokozeki, Isao Abe, Naoto Tonouchi, Yasuko Jojima
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Patent number: 7288388Abstract: The present invention provides a method for producing dipeptide using inexpensively acquirable starting materials by an industrially advantageous and simple pathway. Dipeptide is produced from L-amino acid ester and L-amino acid using a culture of microbes having the ability to produce a dipeptide from an L-amino acid ester and an L-amino acid, using microbial cells isolated from the culture, or a treated microbial cell product of the microbe.Type: GrantFiled: January 26, 2004Date of Patent: October 30, 2007Assignee: Ajinomoto Co., Inc.Inventors: Naoto Tonouchi, Sonoko Suzuki, Kenzo Yokozeki, Hiroyuki Nozaki, Masakazu Sugiyama
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Patent number: 7288401Abstract: The present invention relates to enzymes produced by mutating the genes for a number of subtilases and expressing the mutated genes in suitable hosts are presented. The enzymes exhibit improved wash performance in any detergent in comparison to their wild type parent enzymes.Type: GrantFiled: April 12, 2005Date of Patent: October 30, 2007Assignee: Novozymes A/SInventors: Peter Kamp Hansen, Peter Bauditz, Frank Mikkelsen, Kim Vilbour Andersen
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Patent number: 7241575Abstract: The present invention relates to the identification of novel metallo-proteases (MP) in Gram-positive microorganisms. The present invention provides the nucleic acid and amino acid sequences for Bacillus (MP). The present invention also provides host cells having mutation or deletion of part or all of the gene encoding MP. The present invention also provides host cells further comprising nucleic acid encoding desired heterologous proteins such as enzymes. The present invention also provides cleaning compositions comprising an MP of the present invention.Type: GrantFiled: August 25, 2004Date of Patent: July 10, 2007Assignee: Genecor International, Inc.Inventor: David A. Estell
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Patent number: 7232670Abstract: The present invention is based on the discovery that proteins produced in insect cell cultures are glycosylated in a unique manner that causes them to be selectively imported by cells that express mannose receptors on their membranes, particularly macrophages. Proteins that are selectively imported into cells containing mannose receptors are provided, as well as pharmaceutical compositions containing such proteins and methods for producing such proteins. Application of the present invention to produce proteins useful for treating lysosomal storage disorders is also disclosed. Engineering of cells to express mannose receptors so that they will selectively import proteins produced in insect cells is also taught, as well as a protein targeting system using such cells and proteins. Finally, an improved elution buffer for the purification of proteins produced in insect cells from a Concanavalin A column is provided.Type: GrantFiled: September 28, 2001Date of Patent: June 19, 2007Assignee: St. Jude Children's Research HospitalInventors: Alessandra D'Azzo, Erik Jacobus Bonten
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Patent number: 7220564Abstract: The present invention provides a novel polypeptide efficiently forming (R)-N-benzyl-3-pyrrolidinol, a polynucleotide coding for said polypeptide, and use of the same. The present invention relates to a polypeptide having the following physical and chemical properties (1) to (4): (1) activity: acting on N-benzyl-3-pyrrolidinone with NADH or NADPH as a coenzyme, to form (R)-N-benzyl-3-pyrrolidinol; (2) optimum pH for activity: 5.5 to 6.0; (3) optimum temperature for activity: 50° C. to 55° C.; (4) molecular weight: about 55,000 as determined by gel filtration analysis, about 28,000 as determined by SDS polyacrylamide gel electrophoresis analysis. The present invention also relates to a polypeptide comprising the amino acid sequence shown under SEQ ID NO:1 in the sequence listing, a polynucleotide coding for said polypeptide, and a transformant producing said polypeptide at high levels.Type: GrantFiled: September 19, 2003Date of Patent: May 22, 2007Assignee: Kaneka CorporationInventors: Noriyuki Kizaki, Tozo Nishiyama, Yoshihiko Yasohara
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Patent number: 7220566Abstract: New subtilisin homologues (both nucleic acids and proteins) are provided. Compositions which include these new proteins, recombinant cells, shuffling methods involving the new homologues, antibodies to the new homologues, and methods of using the homologues are also provided.Type: GrantFiled: December 15, 2003Date of Patent: May 22, 2007Assignees: Novozymes A/s, Maxygen, Inc.Inventors: Jon E. Ness, Mark Welch, Lorraine J. Giver, Joel R. Cherry, Torben V. Borchert, Jeremy Minshull
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Patent number: 7208310Abstract: The present invention relates to proteases of high specific activity homologous to proteases derived from Nocardiopsis, and the production thereof by the wild-type, and in recombinant host cells including transgenic plants and non-human transgenic animals. The proteases are effective in animal feed, and detergents. Characteristic structural features of relevance for the specific activity of these proteases of peptidase family S2A or S1E are disclosed.Type: GrantFiled: June 21, 2004Date of Patent: April 24, 2007Assignee: Novozymes A/SInventors: Soeren Flensted Lassen, Carsten Sjoholm, Peter Rahbek Ostergaard
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Patent number: 7192757Abstract: The present invention relates to subtilase variants having a reduced tendency towards inhibition by substances present in eggs, such as trypsin inhibitor type IV-0. In particular, the variants comprise at least one additional amino acid residue between positions 42–43, 51–56, 155–161, 187–190, 216–217, 217–218 or 218–219 (in BASBPN numbering). These subtilase variants are useful exhibiting excellent or improved wash performance on egg stains when used in e.g. cleaning or detergent compositions, such as laundry detergent compositions and dishwash composition, including automatic dishwash compositions. Also, isolated DNA sequences encoding the variants, expression vectors, host cells, and methods for producing and using the variants of the invention. Further, cleaning and detergent compositions comprising the variants are disclosed.Type: GrantFiled: July 2, 2004Date of Patent: March 20, 2007Assignee: Novozymes A/SInventors: Mads Nørregard-Madsen, Line Bloch Larsen, Peter Kamp Hansen
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Patent number: 7192730Abstract: The subject invention pertains to new thermostable enzymes and the use of these enzymes both in proteolysis as well as protein and polypeptide synthesis. The subject invention further concerns polynucleotide sequences which encode the enzymes of the subject invention.Type: GrantFiled: April 15, 2004Date of Patent: March 20, 2007Assignee: University of Florida Research Foundation, Inc.Inventor: David Michael Young
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Patent number: 7132259Abstract: The invention provides, in part, compositions comprising activatable recombinant neurotoxins and polypeptides derived therefrom. The invention also provides, in part, nucleic acid molecules encoding such polypeptides, and methods of making such polypeptides and nucleic acid molecules.Type: GrantFiled: August 25, 2000Date of Patent: November 7, 2006Assignee: Allergan, Inc.Inventors: J. Oliver Dolly, Yan Li, Kuo Chion Chan
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Patent number: 7122514Abstract: Co-administration of a lysostaphin or other anti-staphylococcal agent which cleaves cross-links of peptidoglycans of staphylococci cell walls such as lysostaphin and an antibiotic effective against staphylococci due to antibiotic activity mediated by cell-wall activity is effective against staphylococcal infection, even staphylococci that may be resistant to one or other of lysostaphin or the cell-wall active antibiotic. Co-administration simultaneously suppresses the generation of antibiotic-resistant mutant strains. Effective cell-wall active antibiotics include ?-lactams and glycopeptides.Type: GrantFiled: April 16, 2003Date of Patent: October 17, 2006Assignee: Nutrition 21, Inc.Inventors: Michael Climo, Ellen Murphy, Gordon Archer
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Patent number: 7109015Abstract: The present invention provides methionine aminopeptidases (MetAPs) with a broad substrate range, particularly those capable of removing the N-terminal Met from bulky or acidic penultimate residues. In preferred embodiments, these MetAPs have mutations at the 233, 206 and/or 168 positions of SEQ ID NO:1. Preferably, amino acids at these residues are substituted with glycine or threonine. Also provided are cells comprising the MetAPs, DNA encoding the MetAPs, and methods of using the MetAPs.Type: GrantFiled: March 29, 2004Date of Patent: September 19, 2006Assignee: Academia SinicaInventor: You-Di Liao
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Patent number: 7098017Abstract: The present invention relates to enzymes produced by mutating the genes for a number of subtilases and expressing the mutated genes in suitable hosts are presented. The enzymes exhibit improved autoproteolytic stability in comparison to their wild type parent enzymes.Type: GrantFiled: September 6, 2001Date of Patent: August 29, 2006Assignee: Novozymes A/SInventors: Claus von der Osten, Torben Halkier, Carsten Andersen, Peter Bauditz, Peter Kamp Hansen
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Patent number: 7078372Abstract: The present invention relates to the identification of a novel metalloprotease in gram positive microorganisms. The present invention provides the nucleic acid and amino acid sequences for the metalloprotease. The present invention also provides host cells having a mutation or deletion of part or all of the gene encoding the metalloprotease. The present invention provides host cells which further comprises a nucleic acid encoding desired heterologous proteins such as enzymes. The present invention also provides cleaning compositions, animal feeds and compositions used to treat a textile that include the metalloprotease of the present invention.Type: GrantFiled: August 24, 2004Date of Patent: July 18, 2006Assignee: Genencor International, Inc.Inventor: David A. Estell
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Patent number: 7078377Abstract: Co-administration of a lysostaphin or other anti-staphylococcal agent which cleaves cross-links of peptidoglycans of staphylococci cell walls such as lysostaphin and an antibiotic effective against staphylococci due to antibiotic activity mediated by cell-wall activity is effective against staphylococcal infection, even staphylococci that may be resistant to one or other of lysostaphin or the cell-wall active antibiotic. Co-administration simultaneously suppresses the generation of antibiotic-resistant mutant strains. Effective cell-wall active antibiotics include ?-lactams and glycopeptides.Type: GrantFiled: September 19, 2000Date of Patent: July 18, 2006Assignee: Nutrition 21, Inc.Inventors: Michael Climo, Ellen Murphy, Gordon Archer
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Patent number: 7070986Abstract: The present invention relates to the identification of a novel metalloprotease in gram positive microorganisms. The present invention provides the nucleic acid and amino acid sequences for the metalloprotease. The present invention also provides host cells having a mutation or deletion of part or all of the gene encoding the metalloprotease. The present invention provides host cells which further comprises a nucleic acid encoding desired heterologous proteins such as enzymes. The present invention also provides cleaning compositions, animal feeds and compositions used to treat a textile that include the metalloprotease of the present invention.Type: GrantFiled: August 24, 2004Date of Patent: July 4, 2006Assignee: Genencor International, Inc.Inventor: David A. Estell
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Patent number: 7070990Abstract: The present invention relates to the identification of a novel metalloprotease in gram positive microorganisms. The present invention provides the nucleic acid and amino acid sequences for the metalloprotease. The present invention also provides host cells having a mutation or deletion of part or all of the gene encoding the metalloprotease. The present invention provides host cells which further comprises a nucleic acid encoding desired heterologous proteins such as enzymes. The present invention also provides cleaning compositions, animal feeds and compositions used to treat a textile that include the metalloprotease of the present invention.Type: GrantFiled: August 24, 2004Date of Patent: July 4, 2006Assignee: Genencor International, Inc.Inventor: David A. Estell
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Patent number: 7070819Abstract: The present invention relates to the identification of a novel metalloprotease in gram positive microorganisms. The present invention provides the nucleic acid and amino acid sequences for the metalloprotease. The present invention also provides hot cells having a mutation or deletion of part or all of the gene encoding the metalloprotease. The present invention provides host cells which further comprises a nucleic acid encoding desired heterologous proteins such as enzymes. The present invention also provides cleaning compositions, animal feeds and compositions used to treat a textile that include the metalloprotease of the present invention.Type: GrantFiled: August 24, 2004Date of Patent: July 4, 2006Assignee: Genencor International, Inc.Inventor: David A. Estell
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Patent number: 7026153Abstract: The present invention relates to protease subtilase enzyme, characterized by an insertion in at least one active site loop. The enzymes exhibit improved wash performance in a detergent in comparison to its parent enzyme if it is a subtilase variant.Type: GrantFiled: March 31, 2003Date of Patent: April 11, 2006Assignee: Novozymes A/SInventors: Peter Kamp Hansen, Peter Bauditz, Frank Mikkelsen
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Patent number: 6962703Abstract: The invention relates to an agent specific for peripheral sensory afferents. The agent may inhibit the transmission of signals between a primary sensory afferent and a projection neutron by controlling the release of at least one neurotransmitter or neuromodulator from the primary sensory afferent. The agent may be used in or as a pharmaceutical for the treatment of pain, particularly chronic pain.Type: GrantFiled: May 20, 2002Date of Patent: November 8, 2005Assignees: Ipsen Limited, Health Protection AgencyInventors: Keith Alan Foster, Michael John Duggan, Clifford Charles Shone
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Patent number: 6955886Abstract: The invention provides a scintillation proximity assay for detecting peptidoglycan synthesis. The assay is especially suitable for high throughput screening of compounds affecting peptidoglycan synthesis.Type: GrantFiled: May 4, 1999Date of Patent: October 18, 2005Assignee: AstraZeneca ABInventors: Sunita Desousa, Dwarakanath Prahlad
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Patent number: 6955907Abstract: A novel alkaline protease VapK suitable for a laundry detergent is disclosed. The gene vapk coding for the protease VapK, the recombinant plasmids containing said gene, and the transformed V. metshnikovii KS1 (pSBCm) with said recombinant plasmid are also disclosed. In addition, a process for producing the protease VapK is disclosed.Type: GrantFiled: January 14, 2000Date of Patent: October 18, 2005Assignee: Cheil Jedang CorporationInventors: Ghee Hong Jin, Hyoung Suk Kim, Hyune Mo Rho, Hyune Whan Lee
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Patent number: 6946128Abstract: The present disclosure relates to subtilisin protease conjugate comprising a protease moiety and one or more addition moieties. Each addition moiety is covalently attached to an epitope protection position of the protease moiety. The protease conjugates have decreased immunogenicity relative to a parent protease. The present disclosure further relates to cleaning and personal care compositions comprising the protease conjugates.Type: GrantFiled: July 18, 2000Date of Patent: September 20, 2005Assignee: The Procter & Gamble CompanyInventors: Donn Nelton Rubingh, David John Weisgerber, Paul Elliott Correa
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Patent number: 6939949Abstract: The invention provides novel DNA repair enzymes, genes encoding the enzymes, and methods of using these polypeptides and nucleic acids, including DNA repair.Type: GrantFiled: August 24, 2001Date of Patent: September 6, 2005Assignee: RikenInventors: Seiki Kuramitsu, Shigeyuki Yokoyama
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Patent number: 6929939Abstract: The present invention relates to novel protein variants that exhibit reduced allergenicity when compared to the parental proteins. Also included are DNA molecules that encode the novel variants, host cells comprising the DNA and methods of making proteins less allergenic.Type: GrantFiled: March 22, 2002Date of Patent: August 16, 2005Assignees: Genencor International, Inc., The Procter & Gamble CompanyInventors: David A. Estell, Grant C. Ganshaw, Fiona A. Harding, Edmund A. Larenas, Ayrookaran J. Poulose, Elizabeth E. Sikorski, Elliott P. Russell
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Patent number: 6927055Abstract: Novel protease variants derived from the DNA sequences of naturally-occurring or recombinant non-human proteases are disclosed. The variant proteases, in general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant protease to generate the substitution of a plurality of amino acid residues in the amino acid sequence of a precursor protease. Such variant proteases have properties which are different from those of the precursor protease, such as altered wash performance. The substituted amino acid residue correspond to positions 62, 212, 230, 232, 252 and 257 of Bacillus amyloliquefaciens subtilisin.Type: GrantFiled: August 27, 2002Date of Patent: August 9, 2005Assignee: Genencor International, Inc.Inventors: Ayrookaran J. Poulose, Volker Schellenberger, James T. Kellis, Jr., Christian Paech, Joanne Nadherny, Donald P. Naki, Katherine D. Collier, Robert M. Caldwell, André C. Baeck
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Patent number: 6921657Abstract: The present invention relates to enzymes produced by mutating the genes for a number of subtilases and expressing the mutated genes in suitable hosts are presented. The enzymes exhibit improved wash performance in any detergent in comparison to their wild type parent enzymes.Type: GrantFiled: January 3, 2003Date of Patent: July 26, 2005Assignee: Novozymes A/SInventors: Peter Kamp Hansen, Peter Bauditz, Frank Mikkelsen, Kim Vilbour Andersen
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Patent number: 6913895Abstract: This invention relates to the field of bacterial transglycosylase reactions. The invention is directed to methods for assaying for transglycosylase activity, methods of identifying inhibitors of transglycosylase activity, inhibitors identified by such methods, and methods of identifying the stage of inhibition at which such inhibitors act.Type: GrantFiled: August 17, 1999Date of Patent: July 5, 2005Assignee: Advanced Medicine East, Inc.Inventors: Robert C. Goldman, Arthur A. Branstrom
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Patent number: 6911203Abstract: The present invention relates to in vitro and ex vivo methods of screening for modulators, homologues, and mimetics of lethal factor mitogen activated protein kinase kinase (MAPKK) protease activity, as well as methods of treating cancer by administering LF to transformed cells.Type: GrantFiled: March 5, 2002Date of Patent: June 28, 2005Assignee: The United States of America as represented by the Department of Health and Human ServicesInventors: Nicholas Duesbery, Craig Webb, Stephen Leppla, George Vande Woude
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Patent number: 6908757Abstract: The present invention relates to variants of serine proteases having decreased immunogenicity relative to their corresponding wild-type proteases. More particularly, the present invention relates to variants having a modified amino acid sequence of a wild-type amino acid sequence, wherein the modified amino acid sequence comprises a deletion and, optionally, a substitution of one or more specifically identified positions corresponding to subtilisin BPN?. The invention further relates to mutant genes encoding such variants and cleaning and personal care compositions comprising such variants.Type: GrantFiled: March 26, 1999Date of Patent: June 21, 2005Assignee: The Procter & Gamble CompanyInventors: Donn Nelton Rubingh, Elizabeth Ellen Sikorski, Paul Elliott Correa
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Patent number: 6902922Abstract: New subtilisin homologues (both nucleic acids and proteins) are provided. Compositions which include these new proteins, recombinant cells, shuffling methods involving the new homologues, antibodies to the new homologues, and methods of using the homologues are also provided.Type: GrantFiled: April 2, 2001Date of Patent: June 7, 2005Assignees: Novozymes A/S, Maxygen, Inc.Inventors: Jon E. Ness, Mark Welch, Lorraine J. Giver, Joel R. Cherry, Torben V. Borchert, Willem P. C. Stemmer, Jeremy Minshull