Bacillus Subtilus Or Bacillus Lichenoformis Patents (Class 435/222)
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Patent number: 6929939Abstract: The present invention relates to novel protein variants that exhibit reduced allergenicity when compared to the parental proteins. Also included are DNA molecules that encode the novel variants, host cells comprising the DNA and methods of making proteins less allergenic.Type: GrantFiled: March 22, 2002Date of Patent: August 16, 2005Assignees: Genencor International, Inc., The Procter & Gamble CompanyInventors: David A. Estell, Grant C. Ganshaw, Fiona A. Harding, Edmund A. Larenas, Ayrookaran J. Poulose, Elizabeth E. Sikorski, Elliott P. Russell
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Patent number: 6927055Abstract: Novel protease variants derived from the DNA sequences of naturally-occurring or recombinant non-human proteases are disclosed. The variant proteases, in general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant protease to generate the substitution of a plurality of amino acid residues in the amino acid sequence of a precursor protease. Such variant proteases have properties which are different from those of the precursor protease, such as altered wash performance. The substituted amino acid residue correspond to positions 62, 212, 230, 232, 252 and 257 of Bacillus amyloliquefaciens subtilisin.Type: GrantFiled: August 27, 2002Date of Patent: August 9, 2005Assignee: Genencor International, Inc.Inventors: Ayrookaran J. Poulose, Volker Schellenberger, James T. Kellis, Jr., Christian Paech, Joanne Nadherny, Donald P. Naki, Katherine D. Collier, Robert M. Caldwell, André C. Baeck
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Patent number: 6921657Abstract: The present invention relates to enzymes produced by mutating the genes for a number of subtilases and expressing the mutated genes in suitable hosts are presented. The enzymes exhibit improved wash performance in any detergent in comparison to their wild type parent enzymes.Type: GrantFiled: January 3, 2003Date of Patent: July 26, 2005Assignee: Novozymes A/SInventors: Peter Kamp Hansen, Peter Bauditz, Frank Mikkelsen, Kim Vilbour Andersen
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Patent number: 6908757Abstract: The present invention relates to variants of serine proteases having decreased immunogenicity relative to their corresponding wild-type proteases. More particularly, the present invention relates to variants having a modified amino acid sequence of a wild-type amino acid sequence, wherein the modified amino acid sequence comprises a deletion and, optionally, a substitution of one or more specifically identified positions corresponding to subtilisin BPN?. The invention further relates to mutant genes encoding such variants and cleaning and personal care compositions comprising such variants.Type: GrantFiled: March 26, 1999Date of Patent: June 21, 2005Assignee: The Procter & Gamble CompanyInventors: Donn Nelton Rubingh, Elizabeth Ellen Sikorski, Paul Elliott Correa
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Patent number: 6902922Abstract: New subtilisin homologues (both nucleic acids and proteins) are provided. Compositions which include these new proteins, recombinant cells, shuffling methods involving the new homologues, antibodies to the new homologues, and methods of using the homologues are also provided.Type: GrantFiled: April 2, 2001Date of Patent: June 7, 2005Assignees: Novozymes A/S, Maxygen, Inc.Inventors: Jon E. Ness, Mark Welch, Lorraine J. Giver, Joel R. Cherry, Torben V. Borchert, Willem P. C. Stemmer, Jeremy Minshull
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Patent number: 6897049Abstract: The present invention relates to a novel methods and compositions for producing hyper and hypo allergenic compositions. Specifically, the present invention comprises neutralizing or reducing the ability of T-cells to recognize epitopes and thus prevent sensitization of an individual to the protein. Alternatively, T-cell epitopes are mutated to produce increased immunogenic reactions.Type: GrantFiled: January 23, 2001Date of Patent: May 24, 2005Assignee: Genencor International, Inc.Inventors: David A. Estell, Fiona A. Harding
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Patent number: 6893855Abstract: The present invention relates to subtilase variants having a reduced tendency towards inhibition by substances present in eggs, such as trypsin inhibitor type IV-0. In particular, the variants comprise at least one additional amino acid residue between positions 42-43, 51-56, 155-161, 187-190, 216-217, 217-218 or 218-219 (in BASBPN numbering). These subtilase variants are useful exhibiting excellent or improved wash performance on egg stains when used in e.g. cleaning or detergent compositions, such as laundry detergent compositions and dishwash composition, including automatic dishwash compositions. Also, isolated DNA sequences encoding the variants, expression vectors, host cells, and methods for producing and using the variants of the invention. Further, cleaning and detergent compositions comprising the variants are disclosed.Type: GrantFiled: October 12, 2001Date of Patent: May 17, 2005Assignee: Novozymes A/SInventors: Mads Nørregaard-Madsen, Line Bloch Larsen, Peter Kamp Hansen
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Patent number: 6846656Abstract: The present invention provides a process for economically producing N-acetylneuraminic acid without using expensive materials such as pyruvic acid and phosphoenolpyruvic acid. The process comprises: allowing (i) a culture of a microorganism having N-acetylneuraminic acid aldolase activity or N-acetylneuraminic acid synthetase activity, or a treated matter of the culture, (ii) a culture of a microorganism capable of producing pyruvic acid or a treated matter of the culture, or a culture of a microorganism capable of producing phosphoenolpyruvic acid or a treated matter of the culture, (iii) N-acetylmannosamine, and (iv) an energy source which is necessary for the formation of pyruvic acid or phosphoenolpyruvic acid to be present in an aqueous medium to form and accumulate N-acetylneuraminic acid in the aqueous medium; and recovering N-acetylneuraminic acid from the aqueous medium.Type: GrantFiled: August 25, 2000Date of Patent: January 25, 2005Assignee: Kyowa Hakko Kogyo Co., Ltd.Inventors: Satoshi Koizumi, Kazuhiko Tabata, Tetsuo Endo, Akio Ozaki
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Patent number: 6831053Abstract: The present invention relates to bleaching compositions comprising a protease variant.Type: GrantFiled: April 20, 2000Date of Patent: December 14, 2004Assignee: The Procter & Gamble CompanyInventors: Chanchal Kumar Ghosh, André Cesar Baeck, Ryohei Ohtani, Alfred Busch, Michael Stanford Showell, Ayrookaran J. Poulose, Volker Schellenberger, James T. Kellis, Jr., Christian Paech, Joanne Nadherny, Donald P. Naki, Katherine D. Collier, Robert M. Caldwell
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Publication number: 20040248273Abstract: The present invention relates to subtilase variants having a reduced tendency towards inhibition by substances present in eggs, such as trypsin inhibitor type IV-0. In particular, the variants comprise at least one additional amino acid residue between positions 42-43, 51-56, 155-161, 187-190, 216-217, 217-218 or 218-219 (in BASBPN numbering). These subtilase variants are useful exhibiting excellent or improved wash performance on egg stains when used in e.g. cleaning or detergent compositions, such as laundry detergent compositions and dishwash composition, including automatic dishwash compositions. Also, isolated DNA sequences encoding the variants, expression vectors, host cells, and methods for producing and using the variants of the invention. Further, cleaning and detergent compositions comprising the variants are disclosed.Type: ApplicationFiled: July 2, 2004Publication date: December 9, 2004Applicant: Novozymes A/SInventors: Mads Noerregaard-Madsen, Line Bloch Larsen, Peter Kamp Hansen
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Publication number: 20040241820Abstract: The present invention relates to subtilases having a reduced tendency towards inhibition by substances present in eggs, such as the trypsin inhibitor type IV-0. These subtilases are useful exhibiting excellent or improved wash performance on egg stains when used in e.g. cleaning or detergent compositions, such as laundry detergent compositions and dishwash compositions, including automatic dishwash compositions.Type: ApplicationFiled: August 16, 2001Publication date: December 2, 2004Applicant: Novozymes A/SInventors: Helle Outtrup, Paul Erik Pedersen, Marianne Vind Sorensen
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Patent number: 6818429Abstract: The present invention provides amino acid sequences of peptides that are encoded by genes within the human genome, the protease peptides of the present invention. The present invention specifically provides isolated peptide and nucleic acid molecules, methods of identifying orthologs and paralogs of the protease peptides, and methods of identifying modulators of the protease peptides.Type: GrantFiled: August 7, 2002Date of Patent: November 16, 2004Assignee: Applera CorporationInventors: Weiniu Gan, Karen A. Ketchum, Valentina Di Francesco, Ellen M. Beasley
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Patent number: 6815193Abstract: Novel protease variants derived from the DNA sequences of naturally-occurring or recombinant non-human proteases are disclosed. The variant proteases, in general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant protease to generate the substitution of a plurality of amino acid residues in the amino acid sequence of a precursor protease. Such variant proteases have properties which are different from those of the precursor protease, such as altered wash performance.Type: GrantFiled: November 2, 2001Date of Patent: November 9, 2004Assignee: Genencor International, Inc.Inventors: Ayrookaran J. Poulose, Volker Schellenberger, James T. Kellis, Jr., Christian Paech, Joanne Nadherny, Donald P. Naki, Katherine D. Collier, Robert M. Caldwell, André C. Baeck
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Publication number: 20040209343Abstract: The present invention relates to methods for producing variants of a parent TY145 subtilase and of a parent BPN′ subtilase and to TY145 and BPN′ variants having altered properties as compared to the parent TY145/BPN′ subtilase.Type: ApplicationFiled: February 24, 2004Publication date: October 21, 2004Applicant: Novozymes A/SInventors: Allan Svendsen, Henriette Draborg
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Publication number: 20040203129Abstract: The present invention relates to alkaline proteases having high specific activity and strong oxidant resistance. The present invention also relates to alkaline proteases having excellent detergency that is to be added to a detergent.Type: ApplicationFiled: May 4, 2004Publication date: October 14, 2004Applicant: KAO CORPORATIONInventors: Yuji Hatada, Akinori Ogawa, Yasushi Kageyama, Tsuyoshi Sato, Hiroyuki Araki, Nobuyuki Sumitomo, Mitsuyoshi Okuda, Katsuhisa Saeki
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Patent number: 6780629Abstract: The present invention relates to subtilase enzymes of the I-S1 and I-S2 sub-groups having an additional amino acid in the active site loop (c) region from positions 125 to 132. The variant subtilases of the present invention exhibit improved wash performance in a detergent in comparison to its parent enzyme.Type: GrantFiled: September 13, 2002Date of Patent: August 24, 2004Assignee: Novozymes A/SInventors: Peter Kamp Hansen, Peter Bauditz, Frank Mikkelsen, Kim Vilbour Andersen, Carsten Andersen, Mads Norregaard-Madsen
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Patent number: 6773907Abstract: The present invention relates to subtilase enzymes of the I-S1 and I-S2 sub-groups having an additional amino acid in the active site loop (b) region from positions 95 to 103. The variant subtilases of the present invention exhibit improved wash performance in a detergent in comparison to its parent enzyme.Type: GrantFiled: September 12, 2002Date of Patent: August 10, 2004Inventors: Peter Kamp Hansen, Peter Bauditz, Frank Mikkelsen, Kim Vilbour Andersen, Carsten Andersen, Mads Norregaard-Madsen
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Patent number: 6770469Abstract: A novel enzyme which has an activity to release side chain carboxyl groups and ammonia from a protein by acting upon side chain amido groups in the protein. This invention relates to a method for the production of an enzyme, which comprises culturing in a medium a strain that belongs to a bacterium classified into Cytophagales or Actinomycetes and has the ability to produce an enzyme having a property to deamidate amido groups in protein, thereby effecting production of said enzyme, and subsequently collecting said enzyme from the culture mixture. It also relates to a method for the modification of protein making use of a novel enzyme which directly acts upon amido groups in protein as well as to an enzyme which has a property to deamidate amido groups in protein and a gene which encodes said enzyme.Type: GrantFiled: February 27, 2001Date of Patent: August 3, 2004Assignee: Amano Pharmaceutical Co., Ltd.Inventors: Shotaro Yamaguchi, Akira Matsuura
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Publication number: 20040142837Abstract: An alkaline protease having the following properties; a gene encoding the same; a microorganism producing the same; and washing compositions containing the same; (i) acting over a broad pH value range of 4 to 13 and achieving, at pH 6 to 12, 80% or more the activity at the optimum pH value; (ii) when treated at 40° C. for 30 minutes, being stable over a pH value range of 6 to 11; (iii) having an isoelectric point of about 8.9 to 9.1; and (iv) having casein digesting activity that is not inhibited by oleic acid. The alkaline protease of the present invention is highly stable to various surface active agents and fatty acids, and exhibits high stability to oxidizing agents, and is therefore useful as an enzyme to be used in detergents for automatic dishwashers and laundry detergents, both containing bleaching components.Type: ApplicationFiled: February 24, 2004Publication date: July 22, 2004Applicant: Kao CorporationInventors: Mikio Takaiwa, Mitsuyoshi Okuda, Katsuhisa Saeki, Hiromi Kubota, Jun Hitomi, Yasushi Kageyama, Shitsuw Shikata, Masafumi Nomura
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Patent number: 6759228Abstract: An alkaline protease having the following properties; a gene encoding the same; a microorganism producing the same; and washing compositions containing the same; (i) acting over a broad pH value range of 4 to 13 and achieving, at pH 6 to 12, 80% or more the activity at the optimum pH value; (ii) when treated at 40° C. for 30 minutes, being stable over a pH value range of 6 to 11; (iii) having an isoelectric point of about 8.9 to 9.1; and (iv) having casein digesting activity that is not inhibited by oleic acid. The alkaline protease of the present invention is highly stable to various surface active agents and fatty acids, and exhibits high stability to oxidizing agents, and is therefore useful as an enzyme to be used in detergents for automatic dishwashers and laundry detergents, both containing bleaching components.Type: GrantFiled: August 3, 2001Date of Patent: July 6, 2004Assignee: Kao CorporationInventors: Mikio Takaiwa, Mitsuyoshi Okuda, Katsuhisa Saeki, Hiromi Kubota, Jun Hitomi, Yasushi Kageyama, Shitsuw Shikata, Masafumi Nomura
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Patent number: 6727085Abstract: The present invention relates to the use of a subtilase variant for removal of egg stains from laundry or from hard surfaces, where the subtilase variant comprises at least one additional amino acid residue in the active site loop (b) region from position 95 to 103 (BASBPN numbering). These subtilase variants are useful exhibiting excellent or improved wash performance on egg stains when used in e.g. cleaning or detergent compositions, including automatic dishwash compositions. The present invention also relates to novel subtilase variants, to isolated DNA sequences encoding the variants, expression vectors, host cells, and methods for producing and using the variants of the invention. Further, the present invention relates to cleaning and detergent compositions comprising the variants of the invention.Type: GrantFiled: December 13, 2000Date of Patent: April 27, 2004Inventors: Tina Sejersgård Fanø, Frank Mikkelsen
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Publication number: 20040063177Abstract: The present invention relates to a method of producing novel improved protein mutant which produce low allergenic response in humans compared to the parent of that mutant. Specifically, the present invention comprises neutralizing or reducing the allergenicity of a protein by introducing therein as replacement or modification of an epitope on such protein a sequence from human subtilisin.Type: ApplicationFiled: September 18, 2003Publication date: April 1, 2004Inventor: David A. Estell
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Publication number: 20040023355Abstract: The present invention relates to enzymes produced by mutating the genes for a number of subtilases and expressing the mutated genes in suitable hosts are presented. The enzymes exhibit improved stability and/or improved wash performance in any detergent in comparison to their wild type parent enzymes. The enzymes are well-suited for use in any detergent and for some in especially liquid or solid shaped detergent compositions.Type: ApplicationFiled: July 14, 2003Publication date: February 5, 2004Applicant: Novozymes A/SInventors: Laurens Nicolaas Sierkstra, Jan Klugkist, Peter Markvardsen, Claus Von der Osten, Peter Bauditz
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Publication number: 20040023353Abstract: Novel carbonyl hydrolase variants derived from the DNA sequences of naturally-occurring or recombinant non-human carbonyl hydrolases are disclosed. The variant carbonyl hydrolases, in general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant carbonyl hydrolase to generate the substitution of a plurality of amino acid residues in the amino acid sequence of a precursor carbonyl hydrolase. Such variant carbonyl hydrolases have properties which are different from those of the precursor hydrolase, such as altered proteolytic activity, altered stability, etc.Type: ApplicationFiled: December 18, 2002Publication date: February 5, 2004Inventors: Thomas P. Graycar, Richard R. Bott, Lori J. Wilson
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Patent number: 6682924Abstract: The present invention relates to enzymes produced by mutating the genes for a number of subtilases and expressing the mutated genes in suitable hosts are presented. The enzymes exhibit improved stability and/or improved wash performance in any detergent in comparison to their wild type parent enzymes. The enzymes are well-suited for use in any detergent and for some in especially liquid or solid shaped detergent compositions.Type: GrantFiled: June 5, 2000Date of Patent: January 27, 2004Assignees: Novozymes A/S, UnileverInventors: Laurens Nicolaas Sierkstra, Jan Klugkist, Peter Markvardsen, Claus von der Osten, Peter Bauditz
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Publication number: 20040014175Abstract: Alkalophilic Bacillus strains are provided which efficiently produce mutant Bacillus proteases and which are incapable of expressing the wild-type proteases. Methods for obtaining such Bacillus strains are also disclosed.Type: ApplicationFiled: May 19, 2003Publication date: January 22, 2004Inventors: Johannes Cornelis van der Laan, Christiaan Albertus Gerardus van Eekelen
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Patent number: 6673590Abstract: Novel protease variants derived from the DNA sequences of naturally-occurring or recombinant non-human proteases are disclosed. The variant proteases, in general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant protease to generate the substitution of a plurality of amino acid residues in the amino acid sequence of a precursor protease. Protease variants are provided that contain substitutions of the amino acids at one or more residue positions so that the substitution alters the charge at that position to make the charge more negative or less positive compared to a precursor protease and thus the protease variant is more effective in a low detergent concentration system than a precursor protease.Type: GrantFiled: October 23, 1998Date of Patent: January 6, 2004Assignee: Genencor International, Inc.Inventors: Ayrookaran .J. Poulose, Volker Schellenberger, James T. Kellis, Jr., Christian Paech, Joanne Nadherny, Donald P. Naki, Katherine D. Collier, Robert M. Caldwell
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Publication number: 20040002432Abstract: Provided in the present invention is an alkaline protease wherein an amino acid residue at (a) position 65, (b) position 101, (c) position 163, (d) position 170, (e) position 171, (f) position 273, (g) position 320, (h) position 359 or (i) position 387 of SEQ. ID NO: 1 or at a position corresponding thereto has been selected from the following amino acid residues: position (a): proline, position (b): asparagine, position (c): histidine, aspartic acid, phenylalanine, lysine, asparagine, serine, isoleucine, leucine, glutamine, threonine and valine, position (d): valine and leucine, position (e): alanine, glutamic acid, glycine and threonine, position (f): isoleucine, glycine and threonine, position (g): phenylalanine, valine, threonine, leucine, isoleucine and glycine, position (h): serine, leucine, valine, isoleucine and glutamine, position (i): alanine, lysine, glutamine, glutamic acid, arginine and histidine.Type: ApplicationFiled: March 12, 2003Publication date: January 1, 2004Applicant: KAO CORPORATIONInventors: Mitsuyoshi Okuda, Tsuyoshi Sato, Kazuhiro Saito, Nobuyuki Sumitomo, Yoshifumi Izawa, Katsuhisa Saeki, Tohru Kobayashi, Masafumi Nomura
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Publication number: 20030228995Abstract: Novel protease variants derived from the DNA sequences of naturally-occurring or recombinant non-human proteases are disclosed. The variant proteases, in general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant protease to generate the substitution of a plurality of amino acid residues in the amino acid sequence of a precursor protease. Protease variants are provided that contain substitutions of the amino acids at one or more residue positions so that the substitution alters the charge at that position to make the charge more negative or less positive compared to a precursor protease and thus the protease variant is more effective in a low detergent concentration system than a precursor protease.Type: ApplicationFiled: April 25, 2003Publication date: December 11, 2003Inventors: Ayrookaran J. Poulose, Volker Schellenberger, James T. Kellis, Christian Paech, Joanne Nadherny, Donald P. Naki, Katherine D. Collier, Robert M. Caldwell
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Publication number: 20030215906Abstract: The present invention provides genetically engineered Bacillus strains that can secrete large amount of Bacillus proteases in the extracellular culture medium. More particularly, this invention relates to a process of producing recombinant protease molecules of Bacillus origin in a Bacillus subtilis strain 168, utilizing a strong prophage promoter.Type: ApplicationFiled: May 17, 2002Publication date: November 20, 2003Inventor: Boon Leong Lim
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Patent number: 6632646Abstract: Enzymes produced by mutating the genes for a number of subtilisin proteases and expressing the mutated genes in suitable hosts are presented. The enzymes exhibit improved wash performance in comparison to their wild type parent enzymes. The enzymes are well-suited for use in detergent compositions.Type: GrantFiled: October 2, 2000Date of Patent: October 14, 2003Assignees: Novozymes A/S, Unilever PLC.Inventors: Dorrit Aaslyng, Sven Branner, Sven Hastrup, Leif Nørskov-Lauritsen, Ole Hvilsted Olsen, Merete Simonsen, Eric Casteleijn, Maarten Robert Egmond, Johan Haverkamp, John David Marugg, Arnoldus Theodorus Anthonius Mooren
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Publication number: 20030191039Abstract: The present invention relates to enzymes produced by mutating the genes for a number of subtilisin proteases and expressing the mutated genes in suitable hosts are presented. The enzymes exhibit improved wash performance in comparison to their wild type parent enzymes. The enzymes are well-suited for use in detergent compositions.Type: ApplicationFiled: December 6, 2002Publication date: October 9, 2003Applicant: Novozymes A/SInventors: Dorrit Aaslyng, Sven Branner, Sven Hastrup, Leif Norskov-Lauritsen, Ole Hvilsted, Merete Simonsen, Eric Casteleijn, Maarten Robert Egmond, Johan Haverkamp, John David Marugg, Arnoldus Theodorus Anthonius Mooren
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Publication number: 20030191038Abstract: The present invention relates to subtilase enzymes of the I-S1 and I-S2 sub-groups having an additional amino acid in the active site loop (c) region from positions 125 to 132. The variant subtilases of the present invention exhibit improved wash performance in a detergent in comparison to its parent enzyme.Type: ApplicationFiled: September 13, 2002Publication date: October 9, 2003Applicant: Novozymes A/SInventors: Peter Kamp Hansen, Peter Bauditz, Frank Mikkelsen, Kim Vilbour Andersen, Carsten Andersen, Mads Norregaard-Madsen
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Publication number: 20030186378Abstract: The present invention relates to mutations of a subtilisin gene which result in changes in the chemical characteristics of subtilisin enzymes. Mutations at specific nucleic acids of the subtilisin gene result in amino acid substitutions and consequently, altered enzyme function. Some of these mutant enzymes exhibit physical properties advantageous to industrial applications, particularly in the detergent industry, providing subtilisin which is more stable to oxidation, possesses greater protease activity, and exhibits improved washability.Type: ApplicationFiled: December 5, 2002Publication date: October 2, 2003Applicant: Novozymes A/SInventors: Sven Hastrup, Sven Branner, Fanny Norris, Steffen Bjorn Petersen, Leif Norskov-Lauridsen, Villy Johannes Jensen, Dorrit Aaslyng
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Publication number: 20030180933Abstract: The present invention relates to protease subtilase enzyme, characterized by an insertion in at least one active site loop. The enzymes exhibit improved wash performance in a detergent in comparison to its parent enzyme if it is a subtilase variant.Type: ApplicationFiled: March 31, 2003Publication date: September 25, 2003Applicant: Novozymes A/SInventors: Peter Kamp Hansen, Peter Bauditz, Frank Mikkelsen
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Publication number: 20030180932Abstract: The present invention relates to the identification of novel serine proteases in Gram-positive microorganisms. The present invention provides the nucleic acid and amino acid sequences for the Bacillus subtilis serine proteases SP1, SP2, SP3, SP4 and SP5. The present invention also provides host cells having a mutation or deletion of part or all of the gene encoding SP1, SP2, SP3, SP4 and SP5. The present invention also provides host cells further comprising nucleic acid encoding desired heterologous proteins such as enzymes. The present invention also provides a cleaning composition comprising a serine protease of the present invention.Type: ApplicationFiled: March 26, 2003Publication date: September 25, 2003Inventor: David A. Estell
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Publication number: 20030176304Abstract: The present invention relates to enzymes produced by mutating the genes for a number of subtilases and expressing the mutated genes in suitable hosts are presented. The enzymes exhibit improved wash performance in any detergent in comparison to their wild type parent enzymes.Type: ApplicationFiled: January 3, 2003Publication date: September 18, 2003Applicant: Novozymes A/SInventors: Peter Kamp Hansen, Peter Bauditz, Frank Mikkelsen, Kim Vilbour Andersen
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Publication number: 20030175933Abstract: The present invention relates to mutations of a subtilisin gene which result in changes in the chemical characteristics of subtilisin enzymes. Mutations at specific nucleic acids of the subtilisin gene result in amino acid substitutions and consequently, altered enzyme function. Some of these mutant enzymes exhibit physical properties advantageous to industrial applications, particularly in the detergent industry, providing subtilisin which is more stable to oxidation, possesses greater protease activity, and exhibits improved washability.Type: ApplicationFiled: December 6, 2002Publication date: September 18, 2003Applicant: Novozymes A/SInventors: Sven Hastrup, Sven Branner, Fanny Norris, Steffen Bjorn Petersen, Leif Norskov-Lauridsen, Villy Johannes Jensen, Dorrit Aaslyng
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Publication number: 20030171235Abstract: The present invention relates to subtilase enzymes of the I-S1 and I-S2 sub-groups having an additional amino acid in the active site loop (b) region from positions 95 to 103. The variant subtilases of the present invention exhibit improved wash performance in a detergent in comparison to its parent enzyme.Type: ApplicationFiled: September 12, 2002Publication date: September 11, 2003Applicant: Novozymes A/SInventors: Peter Kamp Hansen, Peter Bauditz, Frank Mikkelsen, Kim Vilbour Andersen, Carsten Andersen, Mads Norregaard-Madsen
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Publication number: 20030166237Abstract: An antifouling paint composition comprising an enzyme, such as endopeptidase, Subtilisin (EC 3.4.21.62) and Alcalase®, and a rosin compound, wherein the enzyme is effective to reduce or prevent fouling by aquatic organisms of a surface coated with the composition. Also disclosed is a method for preventing fouling of a surface by aquatic organisms.Type: ApplicationFiled: February 26, 2003Publication date: September 4, 2003Inventors: Knud Allermann, Ib Schneider
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Patent number: 6610642Abstract: The present invention relates to cleaning compositions comprising a protease variant.Type: GrantFiled: August 6, 2001Date of Patent: August 26, 2003Assignee: The Procter and Gamble CompanyInventors: Chanchal Kumar Ghosh, André Cesar Baeck, Ryohei Ohtani, Alfred Busch, Michael Stanford Showell, Ayrookaran J. Poulose, Volker Schellenberger, James T. Kellis, Jr., Christian Paech, Joanne Nadherny, Donald P. Naki, Katherine D. Collier, Robert M. Caldwell
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Publication number: 20030157645Abstract: The present invention provides subtilisin variants with improved characteristics, including improved substrate affinity, catalytic activity, catalytic efficiency and stability under washing conditions as well as overall wash performance. The subtilisin variants are therefore useful additives in cleaning compositions.Type: ApplicationFiled: December 19, 2002Publication date: August 21, 2003Applicant: DIREVO Bio Tech AG.Inventors: Ulrich Kettling, Andre Koltermann, Oliver Kensch, Rene Kuhlemann, Ulrich Haupts, Markus Rarbach, Konrad Odendahl
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Patent number: 6605458Abstract: A protease subtilase enzyme, characterized by an insertion in at least one active site loop. The enzymes exhibit improved wash performance in a detergent in comparison to its parent enzyme if it is a subtilase variant.Type: GrantFiled: November 19, 1998Date of Patent: August 12, 2003Assignee: Novozymes A/SInventors: Peter Kamp Hansen, Peter Bauditz, Frank Mikkelsen
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Publication number: 20030148495Abstract: The present invention relates to mutations of a subtilisin gene which result in changes in the chemical characteristics of subtilisin enzymes. Mutations at specific nucleic acids of the subtilisin gene result in amino acid substitutions and consequently, altered enzyme function. Some of these mutant enzymes exhibit physical properties advantageous to industrial applications, particularly in the detergent industry, providing subtilisin which is more stable to oxidation, possesses greater protease activity, and exhibits improved washability.Type: ApplicationFiled: November 27, 2002Publication date: August 7, 2003Applicant: Novozymes A/SInventors: Sven Hastrup, Sven Branner, Fanny Norris, Steffen Bjorn Petersen, Leif Norskov-Lauridsen, Villy Johannes Jensen, Dorrit Aaslyng
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Patent number: 6596525Abstract: The present invention relates to a novel improved protein mutant which produces low allergenic response in humans compared to the parent of that mutant. Specifically, the present invention comprises neutralizing or reducing the ability of T-cells to recognize epitopes and thus prevent sensitization of an individual to the protein.Type: GrantFiled: February 23, 1999Date of Patent: July 22, 2003Assignee: Genencor International, Inc.Inventors: David A. Estell, Fiona A. Harding
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Patent number: 6586221Abstract: Novel carbonyl hydrolase variants derived from the DNA sequences of naturally-occurring or recombinant non-human carbonyl hydrolases are disclosed. The variant carbonyl hydrolases, in general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant carbonyl hydrolase to generate the substitution of a plurality of amino acid residues in the amino acid sequence of a precursor carbonyl hydrolase. Such variant carbonyl hydrolases have properties which are different from those of the precursor hydrolase, such as altered proteolytic activity, altered stability, etc.Type: GrantFiled: October 13, 1994Date of Patent: July 1, 2003Assignee: Genencor International, Inc.Inventors: Thomas P. Graycar, Richard R. Bott, Lori J. Wilson
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Patent number: 6586223Abstract: The present invention relates to variants of subtilisin-like proteases having decreased immunogenicity relative to their corresponding wild-type proteases. More particularly, the present invention relates to variants having a modified amino acid sequence of a wild-type amino acid sequence, wherein the modified amino acid sequence comprises a substitution of one or more epitope regions. The invention further relates to mutant genes encoding such variants and cleaning and personal care compositions comprising such variants.Type: GrantFiled: July 18, 2000Date of Patent: July 1, 2003Assignee: The Procter & Gamble CompanyInventors: Elizabeth Ellen Sikorski, Donn Nelton Rubingh
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Patent number: 6586224Abstract: The present invention relates to variants of subtilisin-like proteases having decreased immunogenicity relative to their corresponding wild-type proteases. The present invention further relates to such variants additionally having one or more amino acid substitutions in one or more epitope regions or additionally having one or more stabilizing substitutions. The invention further relates to mutant genes encoding such variants and cleaning and personal care compositions comprising such variants.Type: GrantFiled: July 18, 2000Date of Patent: July 1, 2003Assignee: The Procter & Gamble CompanyInventors: Donn Nelton Rubingh, Elizabeth Ellen Sikorski
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Publication number: 20030113895Abstract: The present invention relates to the identification of novel metallo-proteases (MP) in Gram-positive microorganisms. The present invention provides the nucleic acid and amino acid sequences for Bacillus MP. The present invention also provides host cells having a mutation or deletion of part or all of the gene encoding MP. The present invention also provides host cells further comprising nucleic acid encoding desired heterologous proteins such as enzymes. The present invention also provides cleaning compositions comprising an MP of the present invention.Type: ApplicationFiled: December 23, 2002Publication date: June 19, 2003Inventor: David A. Estell
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Patent number: 6576454Abstract: The present invention relates to modified enzymes with one or more amino acid residues from an enzyme being replaced by cysteine residues, where at least some of the cysteine residues are modified by replacing thiol hydrogen in the cysteine residue with a thiol side chain to form a modified enzyme, wherein the modified enzyme has high esterase and low amidase activity. Also, a method of producing the modified enzymes is provided. The present invention also relates to a method for using the modified enzymes in peptide synthesis.Type: GrantFiled: February 13, 2002Date of Patent: June 10, 2003Assignee: Governing Council of the University of TorontoInventor: J. Bryan Jones