Acting On Carbon To Nitrogen Bond Other Than Peptide Bond (3.5) Patents (Class 435/227)
-
Publication number: 20030082786Abstract: The present invention is directed to uricase modified with polyethylene glycol and to methods of treating different illnesses characterized by increased circulating uric acid levels, including but not limited to, hyperuricemia and tumor lysis syndrome.Type: ApplicationFiled: August 2, 2001Publication date: May 1, 2003Inventors: Charles Mark Ensor, Mike A. Clark, Frederick Wayne Holtsberg
-
Patent number: 6541236Abstract: There are disclosed a protein having an amino acid sequence represented by amino acid numbers 1 to 684 or 49 to 684 shown in SEQ ID NO:2, or a protein having a glutaminase activity in which one or more amino acids is/are deleted from, substituted by, inserted to or added to the amino acid sequence of the above protein; a gene containing DNA encoding the above protein or a gene encoding a protein which hybridizes with the DNA of the above gene under a stringent condition and has a glutaminase activity; a recombinant DNA containing the above gene; a transformant or a transductant containing the above recombinant DNA; and a process for producing glutaminase which comprises culturing the above transformant or the above transductant and collecting glutaminase from a culture medium.Type: GrantFiled: September 6, 2001Date of Patent: April 1, 2003Assignee: Kikkoman CorporationInventors: Kotaro Ito, Genryou Umitsuki, Yasuji Koyama
-
Publication number: 20030054185Abstract: The present invention provides an antimicrobial, controlled-release composition, which includes:Type: ApplicationFiled: September 16, 2002Publication date: March 20, 2003Applicant: CREAVIS GESELLSCHAFT F. TECHN.U.INNOVATION MBHInventors: Peter Ottersbach, Beate Kossman
-
Patent number: 6524844Abstract: The invention discloses a new method for producing enantiomer-enriched 1-amino-4-(hydroxymethyl)-cyclopent-2-ene derivatives of the general formulae (I) and (II) in which R1 is hydrogen or a possibly substituted C1-8 alkyl radical, aryl radical or cycloalkyl radical and R2 is acyl.Type: GrantFiled: June 22, 2001Date of Patent: February 25, 2003Assignee: Lonza AGInventors: Walter Brieden, Kay-Sara Etter, Michael Petersen
-
Patent number: 6525190Abstract: A purified thermostable enzyme is derived from the archael bacterium Thermococcus GU5L5. The enzyme has a molecular weight of about 68.5 kilodaltons and has cellulase activity. The enzyme can be produced from native or recombinant host cells and can be used for the removal of arginine, phenylalanine, or methionine amino acids from the N-terminal end of peptides in peptide or peptidomimetic synthesis. The enzyme is selective for the L, or ‘natural’ enantiomer of the amino acid derivatives and is therefore useful for the production of optically active compounds. These reactions can be performed in the presence of the chemically more reactive ester functionality, a step which is very difficult to achieve with nonenzymatic methods.Type: GrantFiled: October 20, 2000Date of Patent: February 25, 2003Assignee: Diversa CorporationInventors: Dennis Murphy, John Reid, Dan Robertson
-
Patent number: 6514742Abstract: D-aminoacylase derived from fungi is provided. The fungi capable of producing D-aminoacylase include those belonging to the genus Hypomyces, Fusarium, Auricularia, Pythium, and Menisporopsis. The fungal D-aminoacylase is useful for efficiently producing D-amino acids from N-acetyl-D-amino acids.Type: GrantFiled: July 27, 1999Date of Patent: February 4, 2003Assignee: Daicel Chemical Industries, Ltd.Inventors: Kazuya Mitsuhashi, Hiroaki Yamamoto, Akinobu Matsuyama, Shinji Tokuyama
-
Publication number: 20030013102Abstract: The present invention relates to a novel D-hydantoinase from Ochrobactrum anthropi that enantio-selectively hydrolyzes D-hydantoins to their corresponding D-N-carbamoyl-amino acids; nucleic acids that encode for the enzyme; expression vectors including the nucleic acids; and host cells capable of expressing the enzyme.Type: ApplicationFiled: April 3, 2002Publication date: January 16, 2003Inventors: Michael Politino, Sean M. Tonzi, Guna Romancik, John J. Usher, David A. Lowe
-
Patent number: 6506550Abstract: The disclosure describes methods for inducing apoptosis of a selected group of vertebrate cells in vivo by reducing the level of thiamin in the cells. Included are methods for inducing apoptosis of cancer cells. Also described are compounds and compositions for use in methods of thiamin depletion and treating diseases such as cancer, and methods for identifying thiamin-depleting agents and for preparing pharmaceutical compositions.Type: GrantFiled: July 10, 1998Date of Patent: January 14, 2003Assignee: Brandeis UniversityInventors: Chandler Fulton, Elaine Y. Lai
-
Publication number: 20030008360Abstract: The invention relates to a novel process for the preparation of (1R,4S)- or (1S,4R)-1-amino-4-(hydroxymethyl)-2-cyclopentene of the formulae 1Type: ApplicationFiled: November 14, 2001Publication date: January 9, 2003Inventors: Christine Bernegger-Egli, Olwen M. Birch, Pierre Bossard, Walter Brieden, Frank Brux, Knut Burgdorf, Laurent Duc, Kay-Sarah Etter, Yves Guggisberg, Martin Sauter, Eva Maria Urban
-
Patent number: 6500659Abstract: A purified thermostable enzyme is derived from the archael bacterium Thermococcus GU5L5. The enzyme has a molecular weight of about 68.5 kilodaltons and has cellulase activity. The enzyme can be produced from native or recombinant host cells and can be used for the removal of arginine, phenylalanine, or methionine amino acids from the N-terminal end of peptides in peptide or peptidomimetic synthesis. The enzyme is selective for the L, or ‘natural’ enantiomer of the amino acid derivatives and is therefore useful for the production of optically active compounds. These reactions can be performed in the presence of the chemically more reactive ester functionally, a step which is very difficult to achieve with nonenzymatic methods.Type: GrantFiled: October 25, 1999Date of Patent: December 31, 2002Assignee: Diversa CorporationInventors: Dennis Murphy, John Reid, Dan Robertson
-
Patent number: 6465204Abstract: A purified thermostable enzyme is derived from the archael bacterium Thermococcus GU5L5. The enzyme has a molecular weight of about 68.5 kilodaltons and has cellulase activity. The enzyme can be produced from native or recombinant host cells and can be used for the removal of arginine, phenylalanine, or methionine amino acids from the N-terminal end of peptides in peptide or peptidomimetic synthesis. The enzyme is selective for the L, or ‘natural’ enantiomer of the amino acid derivatives and is therefore useful for the production of optically active compounds. These reactions can be performed in the presence of the chemically more reactive ester functionally, a step which is very difficult to achieve with nonenzymatic methods.Type: GrantFiled: June 30, 2000Date of Patent: October 15, 2002Assignee: Diversa CorporationInventors: Dennis Murphy, John Reid, Dan Robertson
-
Publication number: 20020137153Abstract: Enantioselective or enantiospecific nitrilases and nitrile hydratases are used to produce R or S enantiomers of amides, and carboxylic acids. R-amino acids and S-amino acids are produced using such enantioselective enzymes. In addition, methods of producing and screening enantioselective nitrilases and nitrile hydratases are provided.Type: ApplicationFiled: October 4, 2001Publication date: September 26, 2002Inventors: Sandra W. Ramer, Gjalt Huisman, Jim Millis, Roger Sheldon, Stephen delCardayre, Matthew Tobin, Anthony Cox, S. Christopher Davis
-
Patent number: 6428999Abstract: The present invention relates to a novel sphingolipid ceramide N-deacylase (SCDase) having a wide substrate specificity; a method for enzymatically producing a lysosphingolipid or a sphingolipid derivative using the SCDase which is useful in the fields of medicine, carbohydrate engineering, cell engineering, and the like; the lysosphingolipid or sphingolipid derivative obtained by this production method; a gene which encodes a polypeptide having an SCDase activity useful in sphingolipid technology; a method for industrially producing a polypeptide having an SCDase deacylase activity and a recombinant polypeptide thereof using a transformant to which the gene is introduced; a probe or primer which hybridizes to the gene; and an antibody or a fragment thereof which specifically binds to the polypeptide.Type: GrantFiled: September 25, 1998Date of Patent: August 6, 2002Assignee: Takara Shuzo Co., Ltd.Inventors: Makoto Ito, Toyohisa Kurita, Katsuhiro Kita, Noriyuki Sueyoshi, Susumu Mitsutake, Masanori Fujita, Nozomu Okino, Hiroyuki Izu, Ikunoshin Kato
-
Patent number: 6429004Abstract: A purified thermostable enzyme is derived from the archael bacterium Thermococcus GU5L5. The enzyme has a molecular weight of about 68.5 kilodaltons and has cellulase activity. The enzyme can be produced from native or recombinant host cells and can be used for the removal of arginine, phenylalanine, or methionine amino acids from the N-terminal end of peptides in peptide or peptidomimetic synthesis. The enzyme is selective for the L, or ‘natural’ enantiomer of the amino acid derivatives and is therefore useful for the production of optically active compounds. These reactions can be performed in the presence of the chemically more reactive ester functionally, a step which is very difficult to achieve with nonenzymatic methods.Type: GrantFiled: June 30, 2000Date of Patent: August 6, 2002Assignee: Diversa CorporationInventors: Dennis Murphy, John Reid, Dan Robertson
-
Patent number: 6423522Abstract: A lactamase enzyme having good stability, capable of hydrolysing an enantiomer of the bicyclic lactam, 2-azabicyclo[2.2.1]hept-5-en-3-one, to give (−) lactam and (+) amino acid, has been found in a strain of Comamonas acidivorans. The enzyme has been isolated and cloned, and its structure identified.Type: GrantFiled: February 22, 2000Date of Patent: July 23, 2002Assignee: Chirotech Technology, Ltd.Inventors: Richard Anthony Wisdom, Caroline Susan Lee, Robert Christopher Brown
-
Patent number: 6416980Abstract: The present invention relates to a method for producing &agr;-hydroxy acids using an enzyme catalyst having nitrilase activity. More specifically, the invention pertains to use of Acidovorax facilis 72W (ATCC 55746) nitrilase to hydrolyze glycolonitrile to glycolic acid. Glycolonitrile is reacted in an aqueous mixture with a catalyst having Acidovorax facilis 72W nitrilase activity to give glycolic acid selectively, and at high concentration and high yield.Type: GrantFiled: February 23, 2001Date of Patent: July 9, 2002Assignee: E. I. du Pont de Nemours & CompanyInventors: Sarita Chauhan, Robert DiCosimo, Robert D. Fallon, John E. Gavagan, Mark S. Payne
-
Patent number: 6416754Abstract: A genetically-engineered anaerobic organism is provided which, under anaerobic conditions present in a solid tumor, produces an enzyme capable of catalyzing the conversion of a prodrug to its highly cytotoxic product in situ and methods of treating tumors using same.Type: GrantFiled: July 23, 1996Date of Patent: July 9, 2002Assignees: The Board of Trustees of the Leland Stanford Junior University, Microbiological Research Authority (MRA) acting through the Centre for Applied Microbiology and Research (CAMR)Inventors: John Martin Brown, Nigel P. Minton, Amato Giaccia
-
Publication number: 20020086410Abstract: The present invention relates to a novel sphingolipid ceramide N-deacylase (SCDase) having a wide substrate specificity; a method for enzymatically producing a lysosphingolipid or a sphingolipid derivative using the SCDase which is useful in the fields of medicine, carbohydrate engineering, cell engineering, and the like; the lysosphingolipid or sphingolipid derivative obtained by this production method; a gene which encodes a polypeptide having an SCDase activity useful in sphingolipid technology; a method for industrially producing a polypeptide having an SCDase deacylase activity and a recombinant polypeptide thereof using a transformant to which the gene is introduced; a probe or primer which hybridizes to the gene; and an antibody or a fragment thereof which specifically binds to the polypeptide.Type: ApplicationFiled: September 25, 1998Publication date: July 4, 2002Inventors: MAKOTO ITO, TOYOHISA KURITA, KATSUHIRO KITA, NORIYUKI SUEYOSHI, SUSUMU MITSUTAKE, MASANORI FUJITA, NOZOMU OKINO, HIROYUKI IZU, IKUNOSHIN KATO
-
Publication number: 20020064840Abstract: The present invention relates to a method for synthesizing optically active cyanohydrin. An immobilized enzyme is used in the invention, in which (S)-hydroxynitrile lyase is immobilized in a carrier comprising a porous inorganic material.Type: ApplicationFiled: January 12, 2001Publication date: May 30, 2002Applicant: NIPPON SHOKUBAI CO., LTD.Inventors: Hisashi Semba, Yukio Dobashi
-
Patent number: 6383786Abstract: The present invention relates to a method for producing &agr;-hydroxy acids using an enzyme catalyst having nitrilase activity. More specifically, the invention pertains to use of Acidovorax facilis 72W (ATCC 55746) nitrilase to hydrolyze glycolonitrile to glycolic acid. Glycolonitrile is reacted in an aqueous mixture with a catalyst having Acidovorax facilis 72W nitrilase activity to give glycolic acid selectively, and at high concentration and high yield.Type: GrantFiled: September 7, 2001Date of Patent: May 7, 2002Assignee: E. I. du Pont de Nemours and CompanyInventors: Sarita Chauhan, Robert Dicosimo, Robert D. Fallon, John E. Gavagan, Mark S. Payne
-
Publication number: 20020045238Abstract: The invention discloses a novel thermostable D-hydantoinase, and relates to the nucleic acid sequence, amino acid sequence and vector constructs of the enzyme. The thermostable D-hydantoinase of the invention shows about 45%-70% identity in amino acid sequence with other D-hydantoinases. The thermostable D-hydantoinase of the invention converts 5′-substituted D-hydantoinase to the corresponding N-carbamoyl-D- and/or -L-&agr;/&bgr;-amino acids, and retains at least 50% activity after 30 days at 50° C. In addition, the enzyme activity can also enhanced by certain divalent cations.Type: ApplicationFiled: April 18, 2001Publication date: April 18, 2002Inventors: Wen-Hwei Hsu, Chao-Hung Kao
-
Publication number: 20020045739Abstract: The present invention provides a family of bacterial acyl glucosaminylinositol amidases with amidase activity against S-conjugate amides, particularly mycothiol-derived S-conjugate amides. The invention amidases are characterized by a highly conserved 20 amino acid N-terminal region and four highly conserved histidine-containing regions and by having amidase activity, particularly amide hydrolase activity. The invention further provides methods for using the invention amidases in drug screening assays to determine compounds with antibiotic activity or compounds that inhibit activity or production of endogenous acyl glucosaminyl inositol amidase in bacteria. The invention further provides methods for detoxifying a toxic substance by contacting the toxic substance with an invention amidase, for example, by expression of the amidase under environmental conditions in a bacterium.Type: ApplicationFiled: December 7, 2000Publication date: April 18, 2002Inventors: Gerald L. Newton, Yossef Av-Gay, Robert C. Fahey
-
Publication number: 20020045226Abstract: The invention relates to &agr;-galactosidase and to polynucleotides encoding the &agr;-galactosidase. In addition methods of designing new &agr;-galactosidases and method of use thereof are also provided. The &agr;-galactosidases have increased activity and stability at increased pH and temperature.Type: ApplicationFiled: June 20, 2001Publication date: April 18, 2002Inventors: Dennis Murphy, John Reid
-
Patent number: 6361981Abstract: A solution having a high concentration of ammonium (meth)acrylate and which is substantially free of (meth)acrylonitrile is made by enzymatic hydrolysis of (meth)acrylonitrile in the presence of water using an enzyme that has Km for (meth)acrylonitrile below 500 &mgr;m and Ki for ammonium (meth)acrylate about 100,000.Type: GrantFiled: September 19, 2000Date of Patent: March 26, 2002Assignee: Ciba Specialty Chemicals Water Treatments LimitedInventors: Kenneth Charles Symes, Jonathan Hughes
-
Patent number: 6352848Abstract: A novel rec-L-N-carbamoylase from Arthrobacter aurescens and its method of use for producing L-amino acids. The recombinantly produced L-carbamoylase is unexpectedly stable, so that an industrial method of producing L-amino acids can be established with it, in contrast to previously known L-carbamoylases.Type: GrantFiled: April 2, 1999Date of Patent: March 5, 2002Assignees: Degussa-Huls Aktiengesellschaft, Universitat Stuttgart, Roche Diagnostics GmbHInventors: Josef Altenbuchner, Ralf Mattes, Markus Pietzsch, Christoph Syldatk, Anja Wiese, Burkard Wilms
-
Publication number: 20010044120Abstract: An inhibitor of Helicobacter pylori colonization in the stomach comprises as an active ingredient a glycoprotein which specifically binds to H. pylori urease. This glycoprotein is isolated and purified from a glycoprotein-containing substance, especially that derived from bovine milk whey or albumen of chicken eggs by affinity chromatography using a column on which H. pylori urease is immobilized. The glycoprotein is able to effectively inhibit H. pylori colonization, so is useful for the prevention or treatment of diseases caused by infection of H. pylori such as peptic ulcers. A food and medicament comprising the inhibitor are also provided.Type: ApplicationFiled: April 13, 2001Publication date: November 22, 2001Inventors: Yoshikatsu Kodama, Nobutake Kimura
-
Publication number: 20010027183Abstract: The invention provides tdk polypeptides and polynucleotides encoding tdk polypeptides and methods for producing such polypeptides by recombinant techniques. Also provided are methods for utilizing tdk polypeptides to screen for antibacterial compounds.Type: ApplicationFiled: December 28, 2000Publication date: October 4, 2001Inventors: Martin Karl Russel Burnham, Sanjoy Biswas, Alison Francis Chalker, Karen Anne Ingraham, Christopher Michael Traini, Patrick Vernon Warren, Magdalena Zalacain
-
Patent number: 6297040Abstract: Disclosed is the DNA sequence of an enzyme which catalyzes the conversion of chitin to chitosan. The enzyme exhibits substantial homology to the rhizobial nodB protein.Type: GrantFiled: July 12, 1999Date of Patent: October 2, 2001Assignee: Institute for Molecular Biology & Biotechnology/FORTHInventors: George Thireos, Dimitri Kafetzopoulos
-
Patent number: 6287828Abstract: A process for the bioconversion of a nitrile to its corresponding amide product, particularly acrylonitrile to acrylamide which is used for forming polymers. The process uses a thermophilic bacterium having a nitrile hydratase activity that is constitutively expressed, activated by cobalt ions, stable at 60° C., and is most active between 20° C. to 70° C. with optimum activity at 55° C. Alternatively, the process uses the enzyme extracted from the thermophilic bacterium to convert a nitrile to its amide product. The genes encoding nitrile hydratase and amidase are described in which the former is useful for the conversion of an nitrile to its amide and the later is useful for the conversion of an amide to its acid.Type: GrantFiled: April 14, 2000Date of Patent: September 11, 2001Assignee: Board of Trustees operating Michigan State UniversityInventors: Patrick J. Oriel, Rugmini Padmakumar, Sang Hoon Kim
-
Patent number: 6271015Abstract: The soporific activity of cis-9,10-octadecenoamide and other soporific fatty acid primary(amides is neutralized by hydrolysis in the presence of fatty-acid amide hydrolase (FAAH). Hydrolysis of cis-9,10-octadecenoamide by FAAH leads to the formation of oleic acid, a compound without soporific activity. FAAH has be isolated and the gene encoding FAAH has been cloned, sequenced, and used to express recombinant FAAH. Inhibitors of FAAH are disclosed to block the hydrolase activity.Type: GrantFiled: November 4, 1996Date of Patent: August 7, 2001Assignee: The Scripps Research InstituteInventors: Norton B. Gilula, Benjamin F. Cravatt, Richard A. Lerner
-
Patent number: 6251650Abstract: The present invention provides a nitrile hydratase nucleic acid fragment isolated from Pseudomonas putida which encodes a nitrile hydratase activity capable of catalyzing the hydrolysis of certain racemic nitriles to the corresponding R- or S-amides. Also provided are transformed microorganisms capable of the active expression of said nitrile hydratase activity. Additionally, the invention provides a transformant harboring the nitrile hydratase gene in conjunction with an amidase gene, both of which may be co-expressed producing active nitrile hydratase and amidase enzymes respectively. Methods for the production of such enantiomeric materials are also provided.Type: GrantFiled: October 13, 2000Date of Patent: June 26, 2001Assignee: E. I. du Pont de Nemours and CompanyInventors: Robert Donald Fallon, Mark James Nelson, Mark Scott Payne
-
Patent number: 6251651Abstract: A novel enzyme which has an activity to release side chain carboxyl groups and ammonia from a protein by acting upon side chain amido groups in the protein. This invention relates to a method for the production of an enzyme, which comprises culturing in a medium a strain that belongs to a bacterium classified into Cytophagales or Actinomycetes and has the ability to produce an enzyme having a property to deamidate amido groups in protein, thereby effecting production of said enzyme, and subsequently collecting said enzyme from the culture mixture. It also relates to a method for the modification of protein making use of a novel enzyme which directly acts upon amido groups in protein as well as to an enzyme which has a property to deamidate amido groups in protein and a gene which encodes said enzyme.Type: GrantFiled: June 3, 1999Date of Patent: June 26, 2001Assignee: Amano Pharmaceutical Co., Ltd.Inventors: Shotaro Yamaguchi, Akira Matsuura
-
Patent number: 6251625Abstract: The invention concerns a process for the enzymatic preparation of protected di- and oligopeptides and the separation of the protective groups used. The process according to the invention enables peptides to be synthesized simply and economically and the protective group to be separated carefully. The process comprises three reaction steps: 1. Preparation of N-carbamoyl amino acid or N-carbamoyl amino acid derivatives; 2. Formation of the peptide bond between the carbamoyl-protected electrophile and nucelophile; and 3. Separation of the carbamoyl-protective group.Type: GrantFiled: April 3, 1998Date of Patent: June 26, 2001Assignee: Degussa AktiengesellschaftInventors: Andreas Bommarius, Karlheinz Drauz, Uwe Eichhorn, Hans-Dieter Jakubke, Matthias Kottenhahn
-
Patent number: 6248571Abstract: A process for the preparation of dihydroxypyrimidine derivatives of the general formula: in which R1 and R2 are identical or different and are a hydrogen atom, aryl group, or a C1-C4-alkyl group or an aryl group, starting from a compound of the general formula: in which R2 has the meaning mentioned above and R3 is —CN or COOR4, in which R4 is a C1-C4-alkyl group.Type: GrantFiled: February 25, 1998Date of Patent: June 19, 2001Assignee: Lonza AGInventors: Beat Schmidt, Andreas Kiener, John McGarrity
-
Patent number: 6235516Abstract: The invention belongs to the field of biotechnology. It concerns a biocatalyst, i.e. a dead or living microorganism or a polypeptide, preferably in isolated form, which exhibits acylase enzymatic activity without lipase- or esterase-activity. The biocatalyst is capable of stereoselectively hydrolysing a racemic acylamide which has an aliphatic acyl residue and which is not a derivative of a natural amino acid.Type: GrantFiled: November 12, 1998Date of Patent: May 22, 2001Assignee: Novartis AGInventors: Oreste Ghisalba, Matthias Kittelmann, Kurt Laumen, Paula Walser-Volken
-
Patent number: 6214592Abstract: The present invention relates to an enzyme with amidase activity, particularly towards substrates of the oligomer type derived from PA 6.Type: GrantFiled: April 3, 1998Date of Patent: April 10, 2001Assignee: Rhone-Poulenc Fibres et Polymeres S.A.Inventors: Joël Crouzet, Olivier Favre-Bulle, Catherine Jourdat, Anne-Marie Le Coq, Dominique Petre
-
Patent number: 6200767Abstract: The present invention provides a process for eliminating effectively ATP in a sample, using adenosine phosphate deaminase alone or in combination with at least one enzyme from the group consisting of apyrase, alkaline phosphatase, acid phosphatase, hexokinase and adenosine triphosphatase, a process for determining biological cells contained in foods and beverages in convenient and precise manner in combination with bioluminescence method, and a reagent for the determination thereof.Type: GrantFiled: January 5, 1999Date of Patent: March 13, 2001Assignee: Kikkoman CorporationInventors: Tatsuya Sakakibara, Seiji Murakami, Noriaki Hattori, Keiko Yajitate, Teruo Watarai, Motoo Nakajima, Kazuhiro Imai
-
Patent number: 6194190Abstract: To provide an amino terminal protecting group-releasing enzyme characterized in that the enzyme possesses an activity for releasing a protecting group by acting on a peptide of which amino terminal is blocked by the protecting group, and exhibits the activity for two or more protecting groups, or a functional equivalent thereof; a DNA encoding the same; a method for producing the enzyme; a method for removing amino terminal protecting group including the step of subjecting to a reaction with the enzyme to release amino terminal protecting group; and a method for analyzing an amino acid sequence. The above enzyme is useful in the analysis of an amino acid sequence of peptides, particularly proteins and peptides, of which amino terminal is blocked by unknown protecting groups.Type: GrantFiled: December 21, 1998Date of Patent: February 27, 2001Assignee: Takara Shuzo Co., Ltd.Inventors: Yukiko Izu, Tetsuki Tanaka, Masaru Miyagi, Tetsuo Tanigawa, Jun Tomono, Susumu Tsunasawa, Ikunoshin Kato
-
Patent number: 6180387Abstract: The invention provides arginine deiminase polypeptides and DNA (RNA) encoding arginine deiminase polypeptides and methods for producing such polypeptides by recombinant techniques. Also provided are methods for utilizing arginine deiminase polypeptides to screen for antibacterial compounds.Type: GrantFiled: November 5, 1997Date of Patent: January 30, 2001Assignee: SmithKline Beecham CorporationInventors: Sanjoy Biswas, Martin Karl Russel Burnham, Michael Arthur Lonetto, Patrick Vernon Warren, Richard Lloyd Warren
-
Patent number: 6180359Abstract: This invention relates to a process for the preparation of 2-hydroxy-4-methylthiobutyric acid or the ammonium salt of 2-hydroxy-4-methylthiobutyric acid by enzymatic hydrolysis of 2-hydroxy-4-methylthiobutyronitrile, comprising: a) preparing a biological material having a nitrilase activity; b) immobilizing the biological material, c) exposing the 2-hydroxy-4-methylthiobutyronitrile to the biological material thus immobilized to obtain the ammonium salt of 2-hydroxy-4-methylthiobutyric acid; and d) optionally converting the salt obtained to the corresponding acid.Type: GrantFiled: October 24, 1997Date of Patent: January 30, 2001Assignee: Aventis Animal Nutrition S.A.Inventors: Olivier Favre-Bulle, J{acute over (e)}r{circumflex over (o)}me Pierrard, Christophe David, Philippe Morel, Dominique Horbez
-
Patent number: 6171598Abstract: The invention provides ribA polypeptides and polynucleotides encoding ribA polypeptides and methods for producing such polypeptides by recombinant techniques. Also provided are methods for utilizing ribA polypeptides to screen for antibacterial compounds.Type: GrantFiled: January 6, 1999Date of Patent: January 9, 2001Assignees: SmithKline Beecham Corporation, SmithKline Beecham plcInventors: Min Wang, Judith M Ward, Richard Lloyd Warren, Richard Oakley Nicholas, Leslie Marie Palmer, Julie M Pratt, David Justin Charles Knowles, Michael Arthur Lonetto, Jeffrey Mooney, Michael Terence Black, Martin Karl Russell Burnham, Christine Debouck, Jason Craig Fedon, John Edward Hodgson, Deborah Dee Jaworski, Raymond Winfield Reichard, Martin Rosenberg, Christopher Michael Traini, Yi Yi Zhong
-
Patent number: 6162624Abstract: A solution having a high concentration of ammonium (meth)acrylate and which is substantially free of (meth)acrylonitrile is made by enzymatic hydrolysis of (meth)acrylonitrile in the presence of water using an enzyme which has Km for (meth)acrylonitrile below 500 .mu.m and Ki for ammonium (meth)acrylate above 100,000.Type: GrantFiled: April 27, 1998Date of Patent: December 19, 2000Assignee: Ciba Specialty Chemicals Water Treatments LimitedInventors: Kenneth Charles Symes, Jonathan Hughes
-
Patent number: 6156555Abstract: A purified enzyme-I is obtained that participates in C-terminal amidation by acting on a peptide C-terminal glycine adduct to form a peptide C-terminal .alpha.-hydroxyglycine adduct. The enzyme has an optimum pH of about 5 to 7, an optimum temperature of 25 to 40.degree. C. and a molecular weight of about 25 kDa or about 36 kDa, and metal ions and ascorbic acid act as a cofactor. A purified enzyme-II is obtained that participates in C-terminal amidation by acting on the peptide C-terminal .alpha.-hydroxyglycine adduct to produce a C-terminal amidated compound. The enzyme has an optimum pH of about 5 to 6, an optimum temperature of 15 to 35.degree. C. and a molecular weight of about 40 kDa or about 43 kDa. Enzyme-I does not act on the peptide C-terminal .alpha.-hydroxyglycine adduct and enzyme-II does not act on the peptide C-terminal glycine adduct. The enzymes may be purified from a biological material such as horse serum by affinity chromatography using a peptide C-terminal glycine adduct as a ligand.Type: GrantFiled: October 14, 1998Date of Patent: December 5, 2000Assignee: Shiseido Company Ltd.Inventors: Toshii Iida, Toshihiko Kaminuma, Yuka Fuse, Masahiro Tajima, Mitsuo Yanagi, Hiroshi Okamoto, Jiro Kishimoto, Ohji Ifuku, Ichiro Kato
-
Patent number: 6153414Abstract: Cis- and trans-pyrrolopiperidines are advantageously separated into their optical isomers when monoacylating by enzymatic process a mixture containing (R,R)- and (S,S)-pyrrolopiperidine or (S,R)- and (R,S)-pyrrolopiperidine, thereby obtaining a mixture (I) containing (R,R)- and (S,S)-6-acyl-pyrrolopiperidine or (S,R)- and (R,S)-6-acyl-pyrrolopiperidine. Said mixture (I) is then again acylated by enzymatic process, thereby obtaining a mixture (II) containing (S,S)-1,6-diacyl- and (R,R)-6-acyl-pyrrolipiperidine or (S,R)-1,6-diacyl- and (R,S)-6-acyl-pyrrolopiperidine; the enzyme and optionally the solvent and the excess acylating agent are separated from the mixture (II), and the rest is treated with aqueous acid, and the (S,S)-1,6-diacyl-pyrrolopiperidine or the (S,R)-1,6-diacyl-pyrrolopiperidine is separated by extraction and the extraction residue is alkalinized, and the (R,R)-6-acyl-pyrrolopiperidine or the (R,S)-6-acyl-pyrrolopiperidine is separated by extraction.Type: GrantFiled: February 3, 2000Date of Patent: November 28, 2000Assignee: Bayer AktiengesellschaftInventor: Claus Dreisbach
-
Patent number: 6146861Abstract: An amidase or nitrilase is made by continuous culture under carbon limitation using a carbon source which includes, respectively, either (a) an amide or amide precursor or (b) a nitrile or nitrile precursor. Novel enzymes have particular stability. A novel microorganism is Rhodococcus rhodochrous NCIMB 40756 and is capable of producing a particularly stable amidase. The novel amidase, and the amidase made by the defined process, are effective for converting (meth)acrylamide to ammonium (meth)acrylate, for instance in or after the polymerisation of the acrylamide.Type: GrantFiled: July 24, 1998Date of Patent: November 14, 2000Assignee: Ciba Specialty Chemicals Water Treatment LimitedInventors: Yvonne Christine Armitage, Jonathan Hughes
-
Patent number: 6146871Abstract: A procedure for producing a modified 7.beta.-(4-carboxybutanamide) cephalosporinase enzyme which can be purified in a single chromatographic step. The procedure for production of the enzyme involves: mutagenizing the gene which codes for the enzyme from Acinetobacter sp. ATCC 53891 by inserting a nucleotide sequence coding for six histidine residues; fusing the mutant gene with high-efficiency promoter DNA sequences; transforming Escherichia coli cells with the fusion gene construct; growing the transformed Escherichia coli cells; and recovering the enzyme by the use of supports which contain metal chelates. 7-Aminocephalosporanic acid is an important intermediate for the manufacture of a wide range of antibacterial agents of the cephalosporin family.Type: GrantFiled: August 13, 1998Date of Patent: November 14, 2000Assignee: Sntibioticos, S. A.Inventors: Jose Luis Garcia Lopez, Estrella Cortes Rubio, Jose Manuel Guisan Seijas, Jose Luis Barredo Fuente, Bruno Diez Garcia, Alfonso Collados de la Vieja, Alejandro Vitaller Alba, Francisco Salto Maldonado
-
Patent number: 6136583Abstract: A purified thermostable enzyme is derived from the archael bacterium Thermococcus GU5L5. The enzyme has a molecular weight of about 68.5 kilodaltons and has cellulase activity. The enzyme can be produced from native or recombinant host cells and can be used for the removal of arginine, phenylalanine, or methionine amino acids from the N-terminal end of peptides in peptide or peptidomimetic synthesis. The enzyme is selective for the L, or `natural` enantiomer of the amino acid derivatives and is therefore useful for the production of optically active compounds. These reactions can be performed in the presence of the chemically more reactive ester functionality, a step which is very difficult to achieve with nonenzymatic methods.Type: GrantFiled: October 15, 1997Date of Patent: October 24, 2000Assignee: Diversa CorporationInventors: Dennis Murphy, John Reid, Dan Robertson
-
Patent number: 6132713Abstract: A purified arginine deiminase (ADI) obtained from Mycoplasma arthritidis having the amino acid sequence of SEQ ID NO:2 as well as an isolated nucleic acid molecule containing a nucleotide sequence encoding the amino acid sequence set forth in SEQ ID NO:1 are disclosed. Other aspects of the invention include an expression vector, a cloned gene for expressing the Mycoplasma arthritidis derived ADI, (recombinant) host cells useful in expressing the ADI of the present invention and substantially non-antigenic polymer conjugates containing the ADI of the present invention as well as methods of treating arginine deiminase susceptible conditions in mammals. The arginine deiminase-polymer conjugates have high levels of retained enzyme activity and relatively long circulating lives.Type: GrantFiled: March 18, 1999Date of Patent: October 17, 2000Assignee: Enzon, Inc.Inventors: David Ray Fiipula, Maoliang Wang
-
Patent number: 6083719Abstract: The present invention relates to a DNA sequence for the human cytidine deaminase that has been engineered into an eukaryotic expression vector, thereby permitting cytidine deaminase expression in mammalian cells. Cytidine deaminase expression confers resistance to cytosine nucleoside analogs, such as cytosine arabinoside, and can be used as a positive selectable marker. The expression of cytidine deaminase in cells protects them from the toxic effects of cytosine nucleoside analogs. Such a resistance provides applications for gene therapy of malignant, immune and viral diseases. A bacterial expression vector containing the gene can be used to produce cytidine deaminase in large quantities.Type: GrantFiled: October 27, 1997Date of Patent: July 4, 2000Assignee: Hopital Sainte-JustineInventors: Richard L. Momparler, Josee Laliberte, Denis Cournoyer, Nicoletta Eliopoulos
-
Patent number: 6080553Abstract: A creatine amidinohydrolase having the following physicochemical properties:Action: catalyzing the following reaction;creatine+H.sub.2 O.fwdarw.sarcosine+ureaOptimum temperature: about 40-50.degree. C.Optimum pH: pH about 8.0-9.0Heat stability: not more than about 50.degree. C. (pH 7.5, 30 min)Km value for creatine in a coupling assay using a sarcosine oxidase and a peroxidase: about 3.5-10.0 mMMolecular weight: about 43,000 (SDS-PAGE)Isoelectric point: about 3.5,a method for producing said enzyme, comprising culture of microorganism producing said enzyme, a method for the determination of creatine or creatinine in a sample using said enzyme, and a reagent therefor.Type: GrantFiled: February 13, 1997Date of Patent: June 27, 2000Assignee: Toyo Boseki Kabushiki KaishaInventors: Atsushi Sogabe, Takashi Hattori, Yoshiaki Nishiya, Yoshihisa Kawamura