Acting On A Linear Amide Linkage In Linear Amide Patents (Class 435/228)
  • Patent number: 10800808
    Abstract: Described herein are fluid treatment devices for use in tangential flow filtration, comprising a housing unit and a composite material, wherein the composite material comprises: a support member comprising a plurality of pores extending through the support member; and a non-self-supporting macroporous cross-linked gel comprising macropores having an average size of 10 nm to 3000 nm, said macroporous gel being located in the pores of the support member. The invention also relates to a method of separating a substance from a fluid, comprising the step of placing the fluid in contact with an inventive device, thereby adsorbing or absorbing the substance to the composite material contained therein.
    Type: Grant
    Filed: February 8, 2017
    Date of Patent: October 13, 2020
    Assignee: Merck Millipore Ltd.
    Inventors: Damian Brellisford, Donna Lisa Crossley, Greg McIntosh, Robert Ruman, John Rydall, Christopher S. Shields
  • Patent number: 9879076
    Abstract: Novel antibodies, methods and compositions for treatment of a disease which is susceptible to amelioration by the blocking of APP cleavage.
    Type: Grant
    Filed: March 13, 2014
    Date of Patent: January 30, 2018
    Assignee: RAMOT AT TEL AVIV UNIVERSITY LTD.
    Inventors: Sarit Samira, Nurit Rachamim, Michael Tal, Ronald Ellis, Idan Rakover, Rom E. Eliaz, Beka Solomon, Timothy David Jones, Francis Joseph Carr, Polina Rabinovich-Toidman, Meital Sooliman
  • Patent number: 9150775
    Abstract: Method for water tightening of water bearing zones and stabilization of sand in underground constructions, by precipitation of at least one mineral, by introducing into the construction, at the least one aqueous solution of salts comprising Ca2+ ions and urea, and an urease. The urease may be plant based, and made by grounding the plant wherefrom the urease is based, adding water, and soaking at occasional stirring between 2 and 20 h at room temperature. Then the achieved solution is filtrated, and the filtrate is lyophilized. The urease may also be biotechnologically produced by bacteria in an aqueous solution, where after the achieved solution is filtrated, and the filtrate is lyophilized.
    Type: Grant
    Filed: December 20, 2010
    Date of Patent: October 6, 2015
    Assignee: TEMASI AS
    Inventor: Terje Östvold
  • Publication number: 20150147780
    Abstract: Disclosed herein are embodiments for a novel method of producing an organic compound, including harvesting at least one organic compound from an organism or cell line genetically engineered with a gene for at least one proton-pump protein.
    Type: Application
    Filed: November 24, 2014
    Publication date: May 28, 2015
    Inventors: Eugene Dinescu, Vincent Dinescu
  • Publication number: 20150037842
    Abstract: The disclosure relates to a Gram negative bacterial cell that is transformed with a nucleic acid molecule that encodes a Gram positive twin-arginine translocase and including methods for the production of polypeptides.
    Type: Application
    Filed: February 22, 2013
    Publication date: February 5, 2015
    Inventor: Colin Robinson
  • Publication number: 20140335575
    Abstract: An object of the present invention is to provide a means for producing an optically active tropic acid that is a compound useful as a synthetic raw material or an intermediate for pharmaceutical products and the like. The present invention provides a novel polypeptide having activity to (R)-selectively hydrolyze a racemic tropic acid amide, DNA encoding the polypeptide, a vector containing the DNA, a transformant prepared by transformation with the vector, and a method for producing an optically active carboxylic acid amide and an optically active carboxylic acid using them.
    Type: Application
    Filed: September 28, 2012
    Publication date: November 13, 2014
    Inventors: Masutoshi Nojiri, Hiroyuki Kanamaru, Akiko Nishi, Shigeru Kawano, Yoshihiko Yasohara
  • Patent number: 8835467
    Abstract: Compounds are disclosed which inhibit SIR2 base exchange more than deacetylation, thus enhancing SIR2 deacetylation activity. Methods of using the compounds for enhancing SIR2 deacetylation activity and increasing longevity of an organism are also disclosed. Methods for screening for compounds that enhance SIR2 deacetylation activity and increase longevity of an organism are additionally disclosed.
    Type: Grant
    Filed: January 23, 2013
    Date of Patent: September 16, 2014
    Assignee: Albert Einstein College of Medicine of Yeshiva University
    Inventors: Vern L. Schramm, Anthony A. Sauve
  • Publication number: 20140248256
    Abstract: The present invention provides isolated dimeric Streptococcus-specific phage lysins having two Streptococcus-specific phage lysin monomers covalently linked to each other, and having killing activity against one or more Streptococcus bacteria. Also provided for are pharmaceutical compositions of dimeric lysins and their use in therapeutic treatment or alleviation of infections or bacterial colonizations. The dimeric lysins may also be used to decontaminate porous and non-porous surfaces or devices.
    Type: Application
    Filed: October 4, 2012
    Publication date: September 4, 2014
    Inventors: Vincent A. Fischetti, Gregory Resch
  • Patent number: 8728771
    Abstract: The present invention provides a L-succinylacylase consisting of: (a) a protein coded by a gene consisting of a nucleic acid sequence shown in SEQ ID No: 1; (b) a protein consisting of an amino acid sequence shown in SEQ ID No: 2; (c) a protein coded by a polynucleotide which hybridizes under a stringent condition with a nucleic acid sequence which is complementary to the nucleic acid sequence shown in SEQ ID No: 1 and having an L-succinylaminoacylase activity; or (d) a protein which consists of an amino acid sequence where one or several amino acid(s) is/are substituted, deleted, inserted and/or added in the protein consisting of the amino acid sequence shown in SEQ ID No: 2 and has an L-succinylaminoacylase activity. This enzyme is able to produce a sterically bulky unnatural amino acid such as L-tert-leucine etc. which is useful as an intermediate for pharmaceuticals.
    Type: Grant
    Filed: December 10, 2009
    Date of Patent: May 20, 2014
    Assignees: Toyo Boseki Kabushiki Kaisha, Sekisui Medical Co., Ltd.
    Inventors: Atsushi Toda, Sachio Iwai, Yoshiaki Nishiya, Shinya Kumagai
  • Patent number: 8703460
    Abstract: In a method for producing an additive for the enzymatic degradation of mycotoxins, in particular fumonisins, it is provided that at least one nucleic acid sequence of genes corresponding to sequences ID Nos. 1, 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22 and 24 is provided, the at least one nucleic acid sequence is expressed in prokaryotic or eukaryotic host cells, and at least one thus prepared enzyme corresponding to sequences ID Nos. 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23 and 25, or at least one complete recombinant host organism optionally along with a cosubstrate, are used in a vegetable raw material.
    Type: Grant
    Filed: September 18, 2009
    Date of Patent: April 22, 2014
    Assignee: Erber Aktiengesellschaft
    Inventors: Wulf-Dieter Moll, Doris Hartinger, Karin Grieβler, Eva Maria Binder, Gerd Schatzmayr
  • Patent number: 8697383
    Abstract: The object of the present invention is to provide a fluorescent substrate for detecting the enzymatic activity of a nitrile-related enzyme. The present invention provides a compound represented by formula (I) and a fluorescent substrate for detecting the enzymatic activity of a nitrile-related enzyme, which comprises the compound.
    Type: Grant
    Filed: May 25, 2011
    Date of Patent: April 15, 2014
    Assignees: Mitsubishi Rayon Co., Ltd., The University of Tokyo
    Inventors: Yasuteru Urano, Tetsuo Nagano, Tomoe Ohta, Fujio Yu
  • Patent number: 8637277
    Abstract: The present invention provides is a method for producing 3-mercaptopropionic acid from 3-mercaptopropionamide or a salt thereof with the use of an amidase. The method enables the production of 3-mercaptopropionic acid on an industrial scale through an enzymatic reaction.
    Type: Grant
    Filed: April 30, 2010
    Date of Patent: January 28, 2014
    Assignee: Mitsui Chemicals, Inc.
    Inventors: Tadashi Araki, Masayuki Furuya, Hidetoshi Hayashi
  • Publication number: 20130338024
    Abstract: The invention provides histone deacetylase class II nucleic acids and polypeptides, methods and reagents for their use, and related compounds including small molecule libraries containing class II histone deacetylase inhibitors.
    Type: Application
    Filed: May 7, 2013
    Publication date: December 19, 2013
    Applicant: President and Fellows of Harvard College
    Inventors: Christina M. Grozinger, Christian A. Hassig, Stuart L. Schreiber
  • Publication number: 20130216654
    Abstract: The present invention relates to amidase enzymes and a feed or food additive comprising the amidase enzyme capable of degrading ochratoxin.
    Type: Application
    Filed: September 6, 2011
    Publication date: August 22, 2013
    Applicant: DUPONT NUTRITION BIOSCIENCES APS
    Inventors: Shukun Yu, Charlotte Horsmans Poulsen, Soren Dalsgaard, Huaming Wang, Igor Nikolaev
  • Patent number: 8460902
    Abstract: The present invention provides a method of producing optically active amino acids from 5-substituted hydantoin by isolating a hydantoinase gene and an N-carbamyl-L-amino acid hydrolase gene involved in an ability to convert 5-substituted hydantoin or N-carbamylamino acid into optically active amino acids from a microorganism of the genus Microbacterium having the above ability and by improving gene amplification and transcriptional and translational activities thereby preparing a recombinant wherein the amount of the desired enzymes produced is increased. The hydantoinase gene is, for example, a DNA encoding for a protein having a hydantoinase activity, which has the nucleotide sequence of SEQ ID NO:1. The N-carbamyl-L-amino acid hydrolase gene is, for example, a DNA encoding for a protein having an N-carbamyl-L-amino acid hydrolase activity, which has the nucleotide sequence of SEQ ID NO:3.
    Type: Grant
    Filed: February 22, 2008
    Date of Patent: June 11, 2013
    Assignee: Ajinomoto Co., Inc.
    Inventors: Yasuhiro Takenaka, Shunichi Suzuki, Norimasa Onishi, Kenzo Yokozeki
  • Patent number: 8383653
    Abstract: Compounds are disclosed which inhibit SIR2 base exchange more than deacetylation, thus enhancing SIR2 deacetylation activity. Methods of using the compounds for enhancing SIR2 deacetylation activity and increasing longevity of an organism are also disclosed. Methods for screening for compounds that enhance SIR2 deacetylation activity and increase longevity of an organism are additionally disclosed.
    Type: Grant
    Filed: June 30, 2004
    Date of Patent: February 26, 2013
    Assignee: Albert Einstein College of Medicine of Yeshiva University
    Inventors: Vern L. Schramm, Anthony A. Sauve
  • Publication number: 20130005002
    Abstract: Process for producing optically active 3-aminocarboxylic acid ester compounds of general Formula I, and the ammonium salts thereof, in which R1 stands for alkyl, alkoxyalkyl, alkenyl, cycloalkyl, heterocycloalkyl, aryl, or hetaryl, and R2 stands for alkyl, cycloalkyl or aryl, in which an enantiomeric mixture of a simply N-acylated 3-aminocarboxylic acid ester of general formula (I.b), in which R1 and R2 have the meanings given above and R3 stands for hydrogen, alkyl, cycloalkyl or aryl, is submitted to an enantioselective deacylation by adding a polypeptide according to claim 1.
    Type: Application
    Filed: September 24, 2010
    Publication date: January 3, 2013
    Applicant: BASF SE
    Inventors: Bernhard Hauer, Thomas Friedrich, Rainer Stürmer, Nina Schneider, Susanne Krauser, Wolf-Rüdiger Krahnert
  • Patent number: 8252564
    Abstract: The present invention relates to a novel Burkholderia multivorans, an amidase produced from the same, and a method for optical resolution of a racemic mixture using the same, and more particularly to a strain Burkholderia multivorans LG 31-3, an amidase having stereoselective substrate specificity, and a method for optical resolution of a racemic mixture using the same. The amidase produced from the novel Burkholderia multivorans LG 31-3 (KCTC 10920BP) according to the present invention can be useful to produce single enantiomer at a high optical purity since the racemic mixture may be easily optically resolved under enzyme reaction conditions of room temperature and normal pressure.
    Type: Grant
    Filed: April 5, 2007
    Date of Patent: August 28, 2012
    Assignee: LG Chem, Ltd.
    Inventors: Oh-Jin Park, Sang-Hyun Lee
  • Patent number: 8236533
    Abstract: The present invention relates to a process for preparing a compound comprising an ?,? amide linkage between a cysteine moiety and a glutamic acid moiety, such as ?-glutamylcysteine or a ?-glutamylcysteine derivative, the process comprising providing a cysteine derivative, a ?-glutamyl donor and an enzyme capable of transferring the ?-glutamyl group to said cysteine derivative in a reaction environment promoting transfer of the ?-glutamyl group to said cysteine derivative. The invention also relates to compounds comprising an ?,? amide linkage between a cysteine moiety and a glutamic acid moiety, such as ?-glutamylcysteine or a ?-glutamylcysteine derivative, when obtained by processes of the invention, and uses thereof.
    Type: Grant
    Filed: March 31, 2006
    Date of Patent: August 7, 2012
    Assignee: NewSouth Innovations PTY Limited
    Inventors: Wallace John Bridge, Martin Hani Zarka
  • Publication number: 20120184018
    Abstract: The invention relates to orthogonal pairs of tRNAs and aminoacyl-tRNA synthetases that can incorporate the unnatural amino acid sulfotyrosine into proteins produced in eubacterial host cells such as E. coli. The invention provides, for example but not limited to, novel orthogonal aminoacyl-tRNA synthetases, polynucleotides encoding the novel synthetase molecules, methods for identifying and making the novel synthetases, methods for producing proteins containing the unnatural amino acid sulfotyrosine and translation systems.
    Type: Application
    Filed: December 14, 2011
    Publication date: July 19, 2012
    Applicant: The Scripps Research Institute
    Inventors: Chang C. Liu, Peter G. Schultz
  • Publication number: 20120149071
    Abstract: A D-aminoacylase having a high substrate specificity is provided. This D-aminoacylase can produce D-amino acids from N-acetyl-D,L-amino acids conveniently and efficiently at a low cost. A D-aminoacylase produced by a microorganism of genus Defluvibacter; which acts on a N-acetyl-D-amino acid; which has a molecular weight (as determined by electrophoresis) of about 55,000 daltons, and an isoelectric point (as determined by two-dimensional electrophoresis for denatured system) of 5.3; which acts on N-acetyl-D-valine, N-acetyl-D-leucine, and the like, but not on N-acetyl-L-valine, N-acetyl-L-leucine, and the like; which has an optimal temperature of 37° C. (pH 8) and an optimal pH value of 8 to 8.5 at 37° C.; and whose activity is inhibited by Mn2+, Co2+, Ni2+, and Zn2+ each at 1 mmol/L, and by dithiothreitol, 2-mercaptoethanol, o-phenanthroline, and L-cysteine each at 5 mmol/L.
    Type: Application
    Filed: January 6, 2012
    Publication date: June 14, 2012
    Applicants: ISOBE Kimiyasu, DAIICHI PURE CHEMICALS CO., LTD.
    Inventors: Kimiyasu Isobe, Seiki Yamaguchi
  • Publication number: 20120134972
    Abstract: The present invention provides isolated polypeptides comprising a fragment of the amino acid sequence of SEQ ID NO:1, or a variant, derivative or fusion thereof, which is capable of binding specifically to and lysing cells of Clostridium difficile, wherein the polypeptide exhibits greater lytic activity on cells of Clostridium difficile than the polypeptide of SEQ ID NO: 1. The invention further provides means for producing the same, methods for killing bacterial cells such as cells of Clostridium difficile, as well as methods for diagnosing, treating and preventing diseases and conditions associated with infection of the same.
    Type: Application
    Filed: May 26, 2010
    Publication date: May 31, 2012
    Applicant: PLANT BIOSCIENCE LIMITED
    Inventors: Melinda Mayer, Arjan Narbad
  • Publication number: 20120115162
    Abstract: Antibodies that specifically bind to fatty acid amide hydrolases and methods of using the antibodies are provided herein.
    Type: Application
    Filed: April 22, 2010
    Publication date: May 10, 2012
    Applicant: INFINITY PHARMACEUTICALS, INC.
    Inventors: Janid A. Ali, James Brownell, Erin Brophy, Thomas T. Tibbitts
  • Publication number: 20120070843
    Abstract: A novel complex is identified between the NAD-dependent deacetylase, SIRT1 and its novel inhibitor, DBC1. Provided herein are methods to indentify a compound that inhibits the complexation between SIRT1 and DBC1. Exemplary methods comprise contacting either the complexation between DBC1 and SIRT1 with an agent being tested for its ability to inhibit the complexation between SIRT1 and DBC1. Also, provided are methods to identify a compound that increases the complexation between SIRT1 and DBC1. Exemplary methods comprise contacting either the complexation between DBC1 and SIRT1 with an agent being tested for its ability to increase the complexation between SIRT1 and DBC1. Further, methods are provided to increase or decrease SIRT1 activity by contacting the complexation between SIRT1 and DBC1 with a peptide that either decreases or increases the complexation between SIRT1 and DBC1.
    Type: Application
    Filed: October 20, 2011
    Publication date: March 22, 2012
    Inventor: Wei Gu
  • Patent number: 8110382
    Abstract: A process is provided for producing glycolic acid from formaldehyde and hydrogen cyanide. More specifically, heat-treated formaldehyde and hydrogen cyanide are reacted to produce glycolonitrile having low concentrations of impurities. The glycolonitrile is subsequently converted to an aqueous solution of ammonium glycolate using an enzyme catalyst having nitrilase activity derived from Acidovorax facilis 72W (ATCC 57746). Glycolic acid is recovered in the form of the acid or salt from the aqueous ammonium glycolate solution using a variety of methods described herein.
    Type: Grant
    Filed: August 12, 2008
    Date of Patent: February 7, 2012
    Assignee: E. I. du Pont de Nemours and Company
    Inventors: Robert DiCosimo, Anna Panova, Jeffery Scott Thompson, Robert D. Fallon, F. Glenn Gallagher, Thomas Foo, Xu Li, George C. Fox, Joseph J. Zaher, Mark S. Payne, Daniel P. O'Keefe
  • Publication number: 20120021469
    Abstract: The disclosure relates to engineered amidase polypeptides and processes of using the polypeptides for chiral resolution of amino acid amide compounds. The disclosure further relates to the polynucleotides that encode the engineered amidase polypeptides and related vectors, host cells, and methods for making the engineered amidase polypeptides.
    Type: Application
    Filed: March 26, 2010
    Publication date: January 26, 2012
    Applicant: CODEXIS, INC.
    Inventors: Owen Gooding, Robert J. Jones, Gjalt Huisman, Jie Yang, Louis Clark
  • Patent number: 8084238
    Abstract: A process is provided for producing glycolic acid from formaldehyde and hydrogen cyanide. More specifically, heat-treated formaldehyde and hydrogen cyanide are reacted to produce glycolonitrile having low concentrations of impurities. The glycolonitrile is subsequently converted to an aqueous solution of ammonium glycolate using an enzyme catalyst having nitrilase activity derived from Acidovorax facilis 72W (ATCC 57746). Glycolic acid is recovered in the form of the acid or salt from the aqueous ammonium glycolate solution using a variety of methods described herein.
    Type: Grant
    Filed: August 12, 2008
    Date of Patent: December 27, 2011
    Assignee: E. I. du Pont de Nemours and Company
    Inventors: Robert DiCosimo, Anna Panova, Jeffery Scott Thompson, Robert D. Fallon, F. Glenn Gallagher, Thomas Foo, Xu Li, George C. Fox, Joseph J. Zaher, Mark S. Payne, Daniel P. O'Keefe
  • Publication number: 20110311499
    Abstract: The present invention provides reagents and methods for inhibiting bacterial infection and abnormal cell growth, as well as for selection cloning of nucleic acid inserts.
    Type: Application
    Filed: July 12, 2011
    Publication date: December 22, 2011
    Applicant: UNIVERSITY OF WASHINGTON THROUGH ITS CENTER FOR COMMERCIALIZATION
    Inventors: JOSEPH MOUGOUS, ALISTAIR BRIAN RUSSELL, MICHELE LEROUX
  • Publication number: 20110300109
    Abstract: The present invention is based, in part, on our discovery of compositions and methods that can be used to systemically deplete arginine and thereby treat arginine-dependent cancers. Our studies indicate that administering a composition that depletes arginine directly to the patient's small intestine will provide effective treatment for arginine-dependent cancers. Moreover, the methods can be carried out in such a way that various sources of arginine are restricted and side effects are minimized. For example, to deplete arginine, one can not only administer an arginine-depleting enzyme directly to the intestinal lumen but can also inhibit endogenous production of arginine, reduce arginine production by intestinal bacteria and limit arginine intake. To minimize side effects, one can inhibit protein breakdown, which may occur as a compensatory mechanism, provide systemic NO from a nitric oxide donor, provide a pressor peptide, and/or provide prostacycline or an analog thereof.
    Type: Application
    Filed: August 25, 2009
    Publication date: December 8, 2011
    Applicant: Kyon Biotech AG
    Inventors: Slobodan Tepic, Goran Cvetkovic
  • Patent number: 8071343
    Abstract: A process is provided for producing glycolic acid from formaldehyde and hydrogen cyanide. More specifically, heat-treated formaldehyde and hydrogen cyanide are reacted to produce glycolonitrile having low concentrations of impurities. The glycolonitrile is subsequently converted to an aqueous solution of ammonium glycolate using an enzyme catalyst having nitrilase activity derived from Acidovorax facilis 72W (ATCC 57746). Glycolic acid is recovered in the form of the acid or salt from the aqueous ammonium glycolate solution using a variety of methods described herein.
    Type: Grant
    Filed: August 12, 2008
    Date of Patent: December 6, 2011
    Assignee: E. I. du Pont de Nemours and Company
    Inventors: Robert DiCosimo, Anna Panova, Jeffery Scott Thompson, Robert D. Fallon, F. Glenn Gallagher, Thomas Foo, Xu Li, George C. Fox, Joseph J. Zaher, Mark S. Payne, Daniel P. O'Keefe
  • Publication number: 20110244530
    Abstract: The present invention provides an L-aminoacylase which is able to produce L-tert-leucine being useful as an intermediate for pharmaceuticals. A protein which is characterized in being represented by any of the following (a) to (d): (a) a protein coded by a gene consisting of a nucleic acid sequence shown in SEQ ID No: 1; (b) a protein consisting of an amino acid sequence shown in SEQ ID No: 2; (c) a protein coded by a polynucleotide which hybridizes under a stringent condition with a nucleic acid sequence which is complementary to the nucleic acid sequence shown in SEQ ID No: 1 and having an L-succinylaminoacylase activity; and (d) a protein which consists of an amino acid sequence where one or several amino acid (s) is/are substituted, deleted, inserted and/or added in the protein consisting of the amino acid sequence shown in SEQ ID No: 2 and has an L-succinylaminoacylase activity.
    Type: Application
    Filed: May 7, 2009
    Publication date: October 6, 2011
    Applicants: TOYO BOSEKI KABUSHIKI KAISHA, SEKISUI MEDICAL CO., LTD.
    Inventors: Atsushi Toda, Sachio Iwai, Yoshiaki Nishiya, Takeshi Miyata, Aya Oosato, Shinya Kumagai, Toshihide Yamada
  • Publication number: 20110236955
    Abstract: The present invention provides an efficient method of producing an arylacylamidase. In an aspect, the present invention provides (a) a DNA comprising the base sequence shown in the sequence listing under SEQ ID NO:1, or (b) a DNA capable of hybridizing, under stringent conditions, with a DNA comprising the base sequence complementary to the base sequence shown in the sequence listing under SEQ ID NO:1 and coding for a polypeptide having arylacylamidase activity. In another aspect, the invention provides a polypeptide encoded by the DNA mentioned above and having arylacylamidase activity, a vector containing the DNA mentioned above, a transformant which is transformed using the DNA or the vector mentioned above, and a method for producing an arylacylamidase which comprises cultivating the transformant mentioned above and obtaining the arylacylamidase from the culture medium.
    Type: Application
    Filed: September 26, 2005
    Publication date: September 29, 2011
    Applicant: KANEKA CORPORATION
    Inventors: Noriyuki Kizaki, Noriyuki Ito, Yoshihiko Yasohara, Toru Nagasawa
  • Publication number: 20110212120
    Abstract: Nucleic acid encoding a novel bacterial polypeptide ykuR, the isolated ykuR polypeptide, its expression from host cells, and its use in screening for potential antibacterial agents. Inhibition of ykuR activity results in inhibition of bacterial growth.
    Type: Application
    Filed: February 11, 2003
    Publication date: September 1, 2011
    Applicant: VERNALIS (OXFORD) LIMITED
    Inventors: Lloyd George Czaplewski, Andrew Stephen Waller
  • Patent number: 8008052
    Abstract: A process is provided for producing glycolic acid from formaldehyde and hydrogen cyanide. More specifically, heat-treated formaldehyde and hydrogen cyanide are reacted to produce glycolonitrile having low concentrations of impurities. The glycolonitrile is subsequently converted to an aqueous solution of ammonium glycolate using an enzyme catalyst having nitrilase activity derived from Acidovorax facilis 72W (ATCC 57746). Glycolic acid is recovered in the form of the acid or salt from the aqueous ammonium glycolate solution using a variety of methods described herein.
    Type: Grant
    Filed: August 12, 2008
    Date of Patent: August 30, 2011
    Assignee: E.I. du Pont de Nemours and Company
    Inventors: Robert DiCosimo, Anna Panova, Jeffery Scott Thompson, Robert D. Fallon, F. Glenn Gallagher, Thomas Foo, Xu Li, George C. Fox, Joseph J. Zaher, Mark S. Payne, Daniel P. O'Keefe
  • Publication number: 20110178102
    Abstract: Compounds are disclosed which inhibit SIR2 base exchange more than deacetylation, thus enhancing SIR2 deacetylation activity. Methods of using the compounds for enhancing SIR2 deacetylation activity and increasing longevity of an organism are also disclosed. Methods for screening for compounds that enhance SIR2 deacetylation activity and increase longevity of an organism are additionally disclosed.
    Type: Application
    Filed: June 30, 2004
    Publication date: July 21, 2011
    Applicant: ALBERT EINSTEIN COLLEGE OF MEDICINE OF YESHIVA UNIVERSITY
    Inventors: Vern L Schramm, Anthony A. Suave
  • Patent number: 7981655
    Abstract: The document provides modified cytosine deaminases with increased solubility and high levels of DNA cytosine deaminase activity.
    Type: Grant
    Filed: February 6, 2009
    Date of Patent: July 19, 2011
    Assignee: Regents of the University of Minnesota
    Inventors: Reuben S Harris, Hiroshi Matsuo
  • Publication number: 20110165605
    Abstract: An object is to provide a novel method of improving an enzyme capable of deamidating a protein.
    Type: Application
    Filed: August 12, 2009
    Publication date: July 7, 2011
    Applicant: Amano Enzyme Inc.
    Inventors: Ryota Hashizume, Bunzo Mikami, Hirotaka Matsubara, Akiko Matsunaga, Shotaro Yamaguchi
  • Patent number: 7939303
    Abstract: A process is provided for producing glycolic acid from formaldehyde and hydrogen cyanide. More specifically, heat-treated formaldehyde and hydrogen cyanide are reacted to produce glycolonitrile having low concentrations of impurities. The glycolonitrile is subsequently converted to an aqueous solution of ammonium glycolate using an enzyme catalyst having nitrilase activity derived from Acidovorax facilis 72W (ATCC 57746). Glycolic acid is recovered in the form of the acid or salt from the aqueous ammonium glycolate solution using a variety of methods described herein.
    Type: Grant
    Filed: August 12, 2008
    Date of Patent: May 10, 2011
    Assignee: E. I. du Pont de Nemours and Company
    Inventors: Robert DiCosimo, Anna Panova, Jeffery Scott Thompson, Robert D. Fallon, F. Glenn Gallagher, Thomas Foo, Xu Li, George C. Fox, Joseph J. Zaher, Mark S. Payne, Daniel P. O'Keefe
  • Publication number: 20110097768
    Abstract: The present invention relates to a novel Burkholderia multivorans, an amidase produced from the same, and a method for optical resolution of a racemic mixture using the same, and more particularly to a strain Burkholderia multivorans LG 31-3, an amidase having stereoselective substrate specificity, and a method for optical resolution of a racemic mixture using the same. The amidase produced from the novel Burkholderia multivorans LG 31-3 (KCTC 10920BP) according to the present invention can be useful to produce single enantiomer at a high optical purity since the racemic mixture may be easily optically resolved under enzyme reaction conditions of room temperature and normal pressure.
    Type: Application
    Filed: April 5, 2007
    Publication date: April 28, 2011
    Applicant: LG CHEM, LTD.
    Inventors: Oh-Jin Park, Sang-Hyun Lee
  • Patent number: 7858348
    Abstract: The present invention provides genes that encode the N-acetyl-(R,S)-?-amino acid acylases. The N-acetyl-(R,S)-?-amino acid acylases were isolated and purified from bacterial cells and the nucleotide sequences were determined. A host, such as Escherichia coli, was used to construct a high-expression system for these genes. The N-acetyl-(R)-?-amino acid acylase produced by Burkholderia sp. AJ110349 (FERM BP-10366) includes, for example, the protein having the amino acid sequence shown in SEQ. ID. NO. 8. The gene encoding this enzyme includes, for example, the DNA having the nucleotide sequence as shown in SEQ. ID. NO. 7. The N-acetyl-(S)-?-amino acid acylase produced by Burkholderia sp. AJ110349 (FERM BP-10366) includes, for example, the protein having the amino acid sequence shown in SEQ. ID. NO. 10. The gene encoding this enzyme includes, for example, the DNA having the nucleotide sequence shown inshown in SEQ. ID. NO. 9. The N-acetyl-(R)-?-amino acid acylase produced by Variovorax sp.
    Type: Grant
    Filed: July 24, 2007
    Date of Patent: December 28, 2010
    Assignee: Ajinomoto Co., Inc.
    Inventors: Shunichi Suzuki, Yuki Imabayashi, Kunihiko Watanabe, Hisashi Kawasaki, Tsuyoshi Nakamatsu
  • Patent number: 7786281
    Abstract: A method for the production of an enzyme, which comprises culturing in a medium a strain that belongs to a bacterium classified into Cytophagales or Actinomycetes, or a new bacterium Chryseobacterium sp. No. 9670 belonging to the genus Chryseobacterium, and has the ability to produce an enzyme having a property to deamidate amido groups in protein, thereby effecting production of the enzyme, and subsequently collecting the enzyme from the culture mixture and a method for the modification of protein making use of a novel enzyme which directly acts upon amido groups in protein, as well as a gene which encodes the enzyme, a recombinant vector which contains the gene, a transformant transformed with the vector and a method in which the transformant is cultured in a medium to effect production of the protein-deamidating enzyme and then the protein-deamidating enzyme is collected from the culture mixture.
    Type: Grant
    Filed: October 23, 2008
    Date of Patent: August 31, 2010
    Assignee: Amano Enzyme Inc.
    Inventor: Shotaro Yamaguchi
  • Patent number: 7776570
    Abstract: A recombinant microorganism is produced by introducing a DNA encoding an enzyme which hydrolyzes an amido bond of L-amino acid amide, especially L-2-alkylcysteine amide, and L-amino acid is produced by using cells or cell processed product of the obtained microorganism.
    Type: Grant
    Filed: July 17, 2008
    Date of Patent: August 17, 2010
    Assignee: Mitsubishi Gas Chemical Company, Inc.
    Inventors: Yasuhisa Asano, Atsushi Inoue
  • Patent number: 7771961
    Abstract: Modulation of cytochrome c acetylation, e.g., with a SIR polypeptide, enables interventions that modulate lifespan regulation and cell proliferation, e.g., by modulating apoptosis and/or mitochondrial function such as respiration.
    Type: Grant
    Filed: July 10, 2009
    Date of Patent: August 10, 2010
    Assignee: Elixir Pharmaceuticals, Inc
    Inventors: L. Julie Huber, Jonathan M. Solomon
  • Patent number: 7741083
    Abstract: A process is provided for producing glycolic acid from formaldehyde and hydrogen cyanide. More specifically, heat-treated formaldehyde and hydrogen cyanide are reacted to produce glycolonitrile having low concentrations of impurities. The glycolonitrile is subsequently converted to an aqueous solution of ammonium glycolate using an enzyme catalyst having nitrilase activity derived from Acidovorax facilis 72W (ATCC 57746). Glycolic acid is recovered in the form of the acid or salt from the aqueous ammonium glycolate solution using a variety of methods described herein.
    Type: Grant
    Filed: August 12, 2008
    Date of Patent: June 22, 2010
    Assignee: E. I. du Pont de Nemours and Company
    Inventors: Robert DiCosimo, Anna Panova, Jeffery Scott Thompson, Robert D. Fallon, F. Glenn Gallagher, Thomas Foo, Xu Li, George C. Fox, Joseph J. Zaher, Mark S. Payne, Daniel P. O'Keefe
  • Patent number: 7741097
    Abstract: A novel biotechnological process for the preparation of nitriles, starting from amides, is described. Micro-organisms of the genus Amycolatopsis, Actinomadura or Rhodococcus are employed for this process.
    Type: Grant
    Filed: July 28, 2006
    Date of Patent: June 22, 2010
    Assignee: Lonza AG
    Inventors: Karen Tracey Robins, Toru Nagasawa
  • Patent number: 7732172
    Abstract: A process is provided for producing glycolic acid from formaldehyde and hydrogen cyanide. More specifically, heat-treated formaldehyde and hydrogen cyanide are reacted to produce glycolonitrile having low concentrations of impurities. The glycolonitrile is subsequently converted to an aqueous solution of ammonium glycolate using an enzyme catalyst having nitrilase activity derived from Acidovorax facilis 72W (ATCC 57746). Glycolic acid is recovered in the form of the acid or salt from the aqueous ammonium glycolate solution using a variety of methods described herein.
    Type: Grant
    Filed: August 12, 2008
    Date of Patent: June 8, 2010
    Assignee: E. I. du Pont de Nemours and Company
    Inventors: Robert DiCosimo, Anna Panova, Jeffrey Scott Thompson, Robert D. Fallon, F. Glenn Gallagher, Thomas Foo, Xu Li, George C. Fox, Joseph J. Zaher, Mark S. Payne, Daniel P. O'Keefe
  • Patent number: 7723082
    Abstract: The invention concerns novel isolated natural or synthetic polynucleotides and polypeptides coded by said polynucleotides, involved in the synthesis of diketopiperazine derivatives, vectors comprising said polynucleotides, micro-organisms transformed with said polynucleotides, uses of said polynucleotides and said polypeptides, as well as methods for the synthesis of diketopiperazine derivatives, including cyclodipeptides and diketopiperazine derivatives 3- and 6-substituted by ?,?-unsaturated amino acid side chains.
    Type: Grant
    Filed: July 19, 2006
    Date of Patent: May 25, 2010
    Assignees: Commissariat a l'Energie Atomique, Centre National de la Recherche Scientifique
    Inventors: Muriel Gondry, Roger Genet, Sylvie Lautru, Jean-Luc Pernodet
  • Patent number: 7704724
    Abstract: Compositions and methods for catalytic buffering of enzymatic decontamination reactions are provided. Enzymatic decontamination of organophosphorus or organohalogen compounds generates acidic reaction products that precipitously reduce the pH of the medium, thus impairing activity of the decontaminating enzymes. Catalytic buffering, that is, the use of an enzyme to produce ions from a substrate to modulate pH, can provide effective pH control. The compositions provided here include urease enzymes with mutations in the alpha subunit of the urease holoenzyme. These mutant ureases maintain urease activity in the presence of fluoride ions, which are organophosphorus and organohalogen hydrolysis products that otherwise inhibit urease activity. The fluoride-resistant ureases act as effective catalytic buffers during organofluorophosphorus hydrolysis reactions. Methods for using the fluoride-resistant ureases in enzymatic decontamination are also provided.
    Type: Grant
    Filed: January 26, 2006
    Date of Patent: April 27, 2010
    Assignee: The United States of America as represented by the Secretary of the Army
    Inventors: Ilona J. Fry, Joseph J. DeFrank
  • Patent number: 7638617
    Abstract: Various methods are provided for the enzymatic production of glycolic acid from glycolonitrile. These methods include: 1) use of Acidovorax facilis 72W nitrilase mutants having improved nitrilase activity for converting glycolonitrile to glycolic acid, and 2) methods to improve catalyst stability and/or productivity. The methods to improve catalyst stability/productivity include use of reaction stabilizers, running the reactions under substantially oxygen free conditions, and controlling the concentration of substrate in the reaction mixture.
    Type: Grant
    Filed: September 15, 2008
    Date of Patent: December 29, 2009
    Assignee: E.I. du Pont de Nemours and Company
    Inventors: Robert DiCosimo, Anna Panova, Daniel P. O'Keefe, Jeffery Scott Thompson, Mark S. Payne
  • Publication number: 20090311714
    Abstract: The present invention concerns a method for the screening of antibacterial substances comprising a step of determining the ability of a candidate substance to inhibit the activity of a purified enzyme selected from the group consisting of: (i) a D-aspartate ligase comprising a polypeptide having an amino acid sequence possessing at least 50% amino acid identity with an amino acid sequence selected from the group consisting of SEQ ID No 1 to SEQ ID No 10, or a biologically active fragment thereof; and (ii) a L,D-transpeptidase comprising a polypeptide having an amino acid sequence possessing at least 50% amino acid identity with the amino acid sequence of SEQ ID No 11, or a biologically active fragment thereof.
    Type: Application
    Filed: August 1, 2006
    Publication date: December 17, 2009
    Applicants: UNIVERSITE RENE DESCARTES, INSERM (Institut National de la Sante et de la Recherche Medicale)
    Inventors: Jean-Luc Mainardi, Laurent Gutmann, Michel Arthur, Samuel Bellais, Jean Emmanuel Hugonnet, Claudine Mayer, Sabrina Biarotte-Sorin