Acting On A Linear Amide Linkage In Linear Amide Patents (Class 435/228)
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Patent number: 4918012Abstract: This invention relates to a method for producing carnitine comprising contacting, in a reaction medium, carnitinamide with (A) an amidase capable of hydrolyzing carnitinamide to form carnitine or (B) a microorganism containing said amidase, carnitinamide hydrolase and a method for producing same.Type: GrantFiled: August 26, 1987Date of Patent: April 17, 1990Assignee: Kyowa Hakko Kogyo Co., Ltd.Inventors: Kiyoshi Nakayama, Haruo Honda, Yukie Ogawa, Tatsuya Ozawa, Tetsuo Ohta
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Treatment of polyacrylamide gel particles with amidase to reduce residual acrylamide content thereof
Patent number: 4906732Abstract: A substantially dry, particulate polyacrylamide composition having reduced acrylamide monomer content is made by mixing amidase with coarse aqueous gel polymer particles, absorbing the amidase into the particles and subsequently drying the particles.Type: GrantFiled: February 9, 1989Date of Patent: March 6, 1990Assignee: Allied Colloids LimitedInventors: David Farrar, Peter Flesher -
Patent number: 4882280Abstract: Different from conventional uricase products, the uricase of the present invention has outstandingly high thermal stability and is active in a wide range of pH from 5 to 10 for the oxidative decomposition of uric acid undertaken in clinical analysis. The uricase of the invention is produced microbiologically by a thermophilic microorganism belonging to the genus of Bacillus and especially named as Bacillus sp. TB-90 which is a novel species distinguishable from any of the microorganisms belonging to the genus of Bacillus.Type: GrantFiled: May 22, 1986Date of Patent: November 21, 1989Assignee: Sapporo Breweries LimitedInventors: Masachika Takashio, Takahide Chikano, Minoru Kamimura
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Patent number: 4880737Abstract: The invention relates to a process for preparation of L-.alpha.-amino acid and D-.alpha.-amino acid amide from DL-.alpha.-amino-acid amide by contacting the DL-.alpha.-amino acid amide in an aqueous solution with an .alpha.-amino acid amidase containing preparation obtained from a culture of Pseudomonas putida in the presence of traces of bivalent metal ions as activator, characterized in that the aqueous solution also contains a potassium salt selected from the group consisting of potassium sulphate and potassium chloride.The invention further relates to a process for the preparation of L-.alpha.-amino acid and D-.alpha.- amino acid amide starting from the corresponding aldehyde, potassium cyanide and ammoniumsulphate, subsequent treatment with a ketone and potassiumhydroxide and finally subjecting to enzymatic hydrolysis with a preparation obtained from Pseudomonas putida.Type: GrantFiled: October 28, 1985Date of Patent: November 14, 1989Assignee: Stamicarbon B.V.Inventors: Pieter L. Kerkhoffs, Wilhelmus H. J. Boesten
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Patent number: 4877734Abstract: A microbiologically produced .alpha.-acetylamino cinnamic acid acylase and a method for its production from Brevibacterium species NCIB 12246 or NCIB 12247. The new enzyme can be used as constituent of a coupled enzyme system for enzymatic conversions which run via the intermediary stage .alpha.-imino-.beta.-phenylpropionic acid or phenylpyruvic acid.Type: GrantFiled: June 26, 1987Date of Patent: October 31, 1989Assignee: Degussa Aktiengesellschaft and Gesellschaft fur biotechnologische Forschung-mit beschrankter Haftung GBFInventors: Maria-Regina Kula, Werner Hummel, Horst Schutte, Wolfgang Leuchtenberger
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Patent number: 4851342Abstract: A method is described for producing acrylamide from acrylonitrile by the action of a microorganism having nitrilase activity in an aqueous medium, which comprises conducting the reaction in the presence of an alkali metal sulfate at an ionic activity of from 0.004 to 0.01 mole per liter while controlling the pH of said aqueous medium within a range of from 7 to 9 with an alkali hydroxide.Type: GrantFiled: January 18, 1985Date of Patent: July 25, 1989Assignees: Nitto Chemical Industry Co., Ltd., Mitsubishi Rayon Co., Ltd.Inventors: Ichiro Watanabe, Yasuo Ogawa, Susumu Seki
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Patent number: 4812403Abstract: Process for preparing D-2-amino-2,3-dimethylbutyramide and/or L-2-amino-2,3-dimethylbutyric acid, wherein an aqueous solution of DL-2-amino-2,3-dimethylbutyramide is contacted with a preparation containing an aminoacyl amidase which has been obtained from a culture of Mycobacterium neoaurum and in that subsequently D-2-amino-2,3-dimethylbutyramide and/or L-2-amino-2,3-dimethyl-butyric acid is (are) recovered from the resulting hydrolysis mixture. The compound D-2-amino-2,3-dimethylbutyramide is novel.Type: GrantFiled: January 22, 1985Date of Patent: March 14, 1989Assignee: Stamicarbon B.V.Inventors: Wilhelmus H. J. Boesten, Peter J. H. Peters
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Patent number: 4786679Abstract: A water-in-oil emulsion, the aqueous phase of which contains Amidase. An improved method of treating water-in-oil emulsions of acrylamide polymers with Amidase to reduce the acrylamide content thereof which comprises adding the Amidase in the form of a water-in-oil emulsion.Type: GrantFiled: February 13, 1987Date of Patent: November 22, 1988Assignee: Nalco Chemical CompanyInventors: Robert L. Wetegrove, Ralph W. Kaesler, Bhupati Bhattacharyya
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Patent number: 4774179Abstract: A process for preparing a 7-aminocephalosporanic acid compound which comprises treating a cephalosporin C compound with an enzyme-producing microorganism belonging to the genus Pseudomonas or a material obtained by subjecting the microorganism to chemical and/or physical treatment. A direct hydrolysis of the cephalosporin C compound into the 7-aminocephalosporanic acid compound and D-.alpha.-aminoadipic acid can be attained by the microorganism or material.Type: GrantFiled: July 9, 1985Date of Patent: September 27, 1988Assignee: Asahi Kasei Kogyo Kabushiki KaishaInventors: Shigeaki Ichikawa, Keizou Yamamoto, Kenji Matsuyama
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Patent number: 4753882Abstract: Different from conventional urease products, the urease of the present invention has excellent stability, and has a smaller Km value. The urease of this invention is produced microbiologically by a thermophilic microorganism belonging to the genus of Bacillus and especially named as Bacillus sp. TB-90 which is a novel species distinguishable from any of the microorganisms belonging to the genus of Bacillus.Type: GrantFiled: July 28, 1986Date of Patent: June 28, 1988Assignee: Sapporo Breweries LimitedInventors: Masachika Takashio, Yasuo Yoneda, Yutaka Mitani, Takahide Chikano, Minoru Kamimura
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Patent number: 4745067Abstract: L-aminoacylases S.sub.1 and S.sub.2 derived from actinomycetes and having physicochemical characteristics such that it is a L-aminoacylase which acts on a N-acyl-L-amino acid to give a L-amino acid, its substrate profile is wide, and it acts not only on a N-acyl derivative of a natural L-amino acid, but also on a N-acyl derivative of a synthetic L-amino acid, while it does not act on a N-acyl-D-amino acid, a DL-N-acetyl-.alpha.methylbenzylamine and a N-acetyl-D-glucosamine, etc.Type: GrantFiled: April 29, 1986Date of Patent: May 17, 1988Assignees: Microbial Chemistry Research Foundation, Banyu Pharmaceutical Co., Ltd.Inventors: Hamao Umezawa, Tomio Takeuchi, Toshiharu Nagatsu, Masa Hamada, Shuichi Iwadare, Ikuo Matsumoto, Hajime Morishima
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Patent number: 4742114Abstract: A water-in-oil emulsion, the aqueous phase of which contains Amidase. An improved method of treating water-in-oil emulsions of acrylamide polymers with Amidase to reduce the acrylamide content thereof which comprises adding the Amidase in the form of a water-in-oil emulsion.Type: GrantFiled: June 19, 1987Date of Patent: May 3, 1988Assignee: Nalco Chemical CompanyInventors: Robert L. Wetegrove, Ralph W. Kaesler
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Patent number: 4711847Abstract: Secretin, which cannot be prepared directly by genetic engineering because of its carboxylic acid carboxyl-terminus, can be obtained by preparing secretylglycine by genetic engineering and then obtaining secretin therefrom by enzymatic conversion of the terminal glycine radical. The gene for the secretylglycine is synthesized chemically from smaller single-stranded units which are linked enzymatically to give the complete gene, incorporated into a suitable vector and amplified therein, after which the peptide is isolated directly or as a fusion protein and, after cyanogen bromide cleavage, is converted enzymatically into secretin.Type: GrantFiled: August 8, 1984Date of Patent: December 8, 1987Assignee: 501 Hoechst AktiengesellschaftInventors: Wolfgang Konig, Joachim Engels, Eugen Uhlmann, Waldemar Wetekam
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Patent number: 4708934Abstract: Peptidyl-glycine .alpha.-amidating monooxygenase is an enzyme extractable from medullary thyroid carcinoma cell lines and tissue samples, having a molecular mass of about 60,000 to 65,000 daltons. It has been purified so as to exhibit a single, homogeneous, well-defined band using electrophoretic procedures performed on SDS-polyacrylamide gels, and has a specific enzymatic activity of at least 50 mU per mg protein. The free or immobilized enzyme, in the presence of Cu.sup.+2 ions, ascorbate, and oxygen, can be used to prepare an .alpha.-amidated protein from a polypeptide substrate possessing a carboxyl-terminal glycine residue. The purified enzyme can be used as an antigen in order to produce enzyme-specific monoclonal antibodies, and can provide the information necessary to design and construct prokaryotes or other appropriate unicellular organisms or host cells isolated from multicellular organisms which possess peptidyl-glycine .alpha.-amidating capability.Type: GrantFiled: September 27, 1984Date of Patent: November 24, 1987Assignee: Unigene Laboratories, Inc.Inventors: James P. Gilligan, Barry N. Jones
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Patent number: 4705752Abstract: Process for the enzymatic hydrolysis of a D-.alpha.-amino-acid amide to the corresponding D-.alpha.-amino-acid, wherein an aqueous solution of the D-.alpha.-amino-acid amide is contacted with an aminoacylamidase-containing preparation obtained from a culture of Rhodococcus erythropolis or a mutant thereof and the D-.alpha.-amino-acid is subsequently recovered from the hydrolysate obtained.Type: GrantFiled: September 25, 1985Date of Patent: November 10, 1987Assignee: Stamicarbon B.V.Inventors: Wilhelmus H. J. Boesten, Maria J. H. Cals
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Patent number: 4687807Abstract: An improved method for reducing the acrylamide present water-in-oil emulsions of acrylamide polymers with amidase which comprises adding the amidase to the water-in-oil emulsion of the acrylamide polymer upon completion of its polymerization and then subjecting the thus treated water-in-oil emulsion to one or more of the following steps:(a) pH adjustment(b) heating(c) inert gas sparging(d) adding a chemical reducing agent.Type: GrantFiled: February 13, 1987Date of Patent: August 18, 1987Assignee: Nalco Chemical CompanyInventors: Robert L. Wetegrove, Ralph W. Kaesler
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Patent number: 4668625Abstract: The invention describes a process for preparing peptides. More particularly, a process for preparing L-.alpha.-aspartyl-L-phenylalanine C.sub.1 -C.sub.4 lower alkyl ester is described and claimed by enzymatic hydrolysis of a suitable N-acyl-derivative.Type: GrantFiled: August 7, 1984Date of Patent: May 26, 1987Assignee: Farmitalia Carlo ErbaInventors: Stefano Cambiaghi, Franco Dallatomasina, Pietro Giardino, Enzo Murador, Gaspare Spreafico
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Patent number: 4645739Abstract: The present invention provides a process for the determination of N-carbamoylsarcosine, wherein a sample solution containing N-carbamoylsarcosine is reacted with N-carbamoylsarcosine-amidohydrolase to give sarcosine, which is then determined.The present invention also provides the enzyme N-carbamoylsarcosine-amidohydrolase, a process for obtaining it and a reagent containing it.Type: GrantFiled: December 16, 1983Date of Patent: February 24, 1987Assignee: Boehringer Mannheim GmbHInventors: Rolf Deeg, Albert Roder, Joachim Siedel, Helmgard Gauhl, Joachim Ziegenhorn
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Patent number: 4637982Abstract: In a process for hydrating a nitrile having 2 to 4 carbon atoms by a microbiological treatment to convert the nitrile into the corresponding amide, it is possible to biologically prepare the corresponding amide effectively by utilizing a microorganism of the genus Pseudomonas which is capable of hydrating the nitrile to convert the same into the corresponding amide. In particular, it is possible to obtain acrylamides which are highly useful polymers by the very simple and energy-saving process in accordance with the present invention.Type: GrantFiled: July 5, 1983Date of Patent: January 20, 1987Assignees: Hideaki Yamada, Nitto Kagaku Kogyo Kabushiki KaishaInventors: Hideaki Yamada, Yoshiki Tani
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Patent number: 4532214Abstract: Aminoacylase is isolated from mammal kidneys by comminuting and homogenizing mammal kidney in water, centrifuging to form an aqueous extract, heating the aqueous extract at 60.degree. to 80.degree. C. for 5 to 15 minutes, centrifuging, adding a salt such as ammonium sulfate to the resultant supernatant, centrifuging to separate solids, dissolving the solids in water, dialyzing and recovering active aminoacylase from the dialyzed solution. The process produces aminoacylase with high specific activity and the process requires fewer purification steps. The aminoacylase obtained may be utilized directly without any subsequent purification to produce immonobilized aminoacylase. Immobilization can be carried out by covalent bonding of the aminoacylase to a partially hydrolyzed Akrilex P type acryl amide-N,N'-methylene-bis(arylamide) copolymer. The recovered aminoacylase can be subjected to two chromatographic purification steps to produce a very pure aminoacylase.Type: GrantFiled: June 3, 1982Date of Patent: July 30, 1985Assignee: Reanal FinomvegyszergyarInventors: Bela Szajani, Janosne Kiss, Jozsefne Ivony, Iren Huber, Laszlo Boros, Ivan Daroczi
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Patent number: 4438201Abstract: A novel enzyme, amidohydrolase, having the ability to depantothenylate antibiotic OA-6129A represented by the following formula ##STR1## but no substantial ability to deacetylate antibiotic PS-5 represented by the following formula ##STR2##Type: GrantFiled: July 1, 1982Date of Patent: March 20, 1984Assignee: Sanraku-Ocean Co., Ltd.Inventors: Katsuro Kubo, Yasuo Fukagawa, Tomoyuki Ishikura
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Patent number: 4430433Abstract: A process for the production of an aryl acylamidase enzyme involves culturing bacteria of one of the strains Pseudomonas fluorescens ATCC 39005 (or suitable mutants or variants thereof) or Pseudomonas putida ATCC 39004 (or suitable mutants or variants thereof) in a culture medium in which the bacterial strains produce aryl acylamidase and collecting the enzyme containing material, generally the cell material. Preferably the resulting cells are then disrupted, especially by enzymatic treatment, and the aryl acylamidase is separated from the other unwanted substances, generally the other cell constituents.Preferably the culture medium contains N-acylaniline, especially N-acetyl aniline. The N-acylaniline may form part of a complex or defined salts medium.Type: GrantFiled: December 1, 1981Date of Patent: February 7, 1984Assignee: Public Health Laboratory Service BoardInventors: Peter M. Hammond, Christopher P. Price, Michael D. Scawen
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Patent number: 4330626Abstract: In a method of preparing the enzyme, urease, from jack beans, wherein the jack beans are reduced to a fine particle size and the fine particles so reduced constitute urease, the fine jack-bean particles thereafter irradiated for a period of time, the irradiated jack beans then recovered and employed as a source of urease enzyme, the improvement which comprises: irradiating the whole jack beans prior to any size reduction of the seeds of the jack beans, to provide a urease derived from the white seeds of the irradiated whole jack beans having an improved activity level.Type: GrantFiled: March 19, 1980Date of Patent: May 18, 1982Assignee: The Enzyme Center, Inc.Inventors: Henry E. Blair, Stanley E. Charm, Richard P. Crowley
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Patent number: 4282322Abstract: This invention relates to two new antibiotics, desacetyl 890A.sub.1 and desacetyl 890A.sub.3, active against both gram-positive and gram-negative bacteria, which are produced by treating 890A.sub.1 and 890A.sub.3, respectively, with an N-acetylthienamycin amidohydrolase produced by a soil microorganism isolated by enrichment techniques. This invention also relates to the process whereby N-acetylated structures of the thienamycin class of antibiotics such as N-acetyl thienamycin can be enzymatically deacetylated.Type: GrantFiled: August 27, 1979Date of Patent: August 4, 1981Assignee: Merck & Co., Inc.Inventors: Jean S. Kahan, Frederick M. Kahan
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Patent number: 4264734Abstract: This invention relates to the new antibiotic, desacetyl 890A.sub.10, active against both gram-positive and gram-negative bacteria, which is produced by treating 890A.sub.10 with an N-acetyl-890A.sub.10 amidohydrolase produced by a soil microorganism isolated by enrichment techniques. This invention also relates to the process by which 890A.sub.10 is enzymatically deacetylated.Type: GrantFiled: September 11, 1979Date of Patent: April 28, 1981Assignee: Merck & Co., Inc.Inventors: Jean S. Kahan, Frederick M. Kahan