Isomerase (5. ) Patents (Class 435/233)
  • Patent number: 10227618
    Abstract: The present application relates to a microorganism of the genus Saccharomyces producing lactic acid and a method for preparing lactic acid using the same. More specifically, the present application relates to a microorganism of the genus Saccharomyces producing lactic acid, wherein the microorganism is modified to weaken or inactivate the activity of pyruvate decarboxylase (PDC) compared to its endogenous activity, to introduce the activity of ATP-citrate lyase (ACL), and to enhance pyruvate biosynthetic pathway compared to its endogenous biosynthetic pathway, and a method for producing lactic acid using the microorganism.
    Type: Grant
    Filed: June 10, 2016
    Date of Patent: March 12, 2019
    Assignee: CJ CHEILJEDANG CORPORATION
    Inventors: Seon Hye Kim, Tae Hee Lee, Young Lyeol Yang, Eun Bin Yang, Kyungsu Na, Cheol Woong Ha
  • Patent number: 10196626
    Abstract: A hexuronate C4-epimerase with improved conversion activity and a method for producing D-tagatose using the hexuronate C4-epimerase. The hexuronate C4-epimerase includes an amino acid sequence set forth in SEQ ID NO: 1, in which serine (S) at position 125, serine (S) at position 185, valine (V) at position 267, serine (S) at position 268, threonine (T) at position 272, tryptophan (W) at position 306, arginine (R) at position 386 and tyrosine (Y) at position 403 from an N-terminal of hexunorate C4-epimerase are mutated.
    Type: Grant
    Filed: July 29, 2016
    Date of Patent: February 5, 2019
    Assignee: CJ CHEILJEDANG CORPORATION
    Inventors: Sung Jae Yang, Yang Hee Kim, Il Hyang Park, Young Mi Lee, Hyun Kug Cho, Seong Bo Kim, Seung Won Park
  • Patent number: 10190112
    Abstract: The present invention relates to novel mutants with cyclase activity and use thereof in a method for biocatalytic cyclization of terpenes, such as in particular for the production of isopulegol by cyclization of citronellal; a method for the preparation of menthol and methods for the biocatalytic conversion of further compounds with structural motifs similar to terpene.
    Type: Grant
    Filed: August 11, 2016
    Date of Patent: January 29, 2019
    Assignee: BASF SE
    Inventors: Michael Breuer, Bernhard Hauer, Dieter Jendrossek, Gabriele Siedenburg, Juergen Pleiss, Demet Sirim, Silvia Fadenrecht
  • Patent number: 9752170
    Abstract: The present invention is directed towards genetic modification of native gene encoding for D-tagatose 3-epimerase and rhamnose isomerase to substantially increase the expression level of these enzymes and use of the enzymes in a process to produce rare monosaccharides such as psicose and allose. Also disclosed in the present invention is expression constructs comprising the modified genes and a host cells to express the same.
    Type: Grant
    Filed: April 17, 2013
    Date of Patent: September 5, 2017
    Assignee: Petiva Private Limited
    Inventors: Saravanakumar Iyappan, Samir Kumar Roy, Saju Varghese, Aparna Devi Addala, Venkata Narayanan Karthikeyan, Kanumuru Rahul Raju, Banibrata Pandey
  • Patent number: 9447404
    Abstract: The present invention relates to novel mutants with cyclase activity and use thereof in a method for biocatalytic cyclization of terpenes, such as in particular for the production of isopulegol by cyclization of citronellal; a method for the preparation of menthol and methods for the biocatalytic conversion of further compounds with structural motifs similar to terpene.
    Type: Grant
    Filed: December 4, 2014
    Date of Patent: September 20, 2016
    Assignee: BASF SE
    Inventors: Michael Breuer, Bernhard Hauer, Dieter Jendrossek, Gabrielle Siedenburg, Juergen Pleiss, Demet Sirim, Silvia Fadenrecht
  • Patent number: 9382523
    Abstract: The present invention provides a transformed or transfected Trichoderma reesei cell comprising one or more of: (i) at least one heterologous nucleotide sequence encoding a lipolytic enzyme comprising an amino acid sequence shown as SEQ ID NO: 1 or SEQ ID NO: 2, (ii) at least one heterologous nucleotide sequence encoding a lipolytic enzyme wherein the nucleotide sequence comprises the nucleotide sequence shown as SEQ ID NO: 3 or SEQ ID NO: 4, or (iii) at least one heterologous nucleotide sequence encoding a lipolytic enzyme wherein the nucleotide sequence comprises the nucleotide sequence which hybridizes to SEQ ID NO: 3 or SEQ ID NO: 4 or a nucleotide sequence which is at least 40% sequence identity to SEQ ID NO: 3 or SEQ ID NO: 4; and culturing the cell under conditions to allow for expression of the heterologous nucleotide sequence(s) encoding the lipolytic enzyme.
    Type: Grant
    Filed: November 7, 2014
    Date of Patent: July 5, 2016
    Assignee: DUPONT NUTRITION BIOSCIENCES APS
    Inventors: Susan Madrid, Cherry Lin, Masoud Rajabi Zargahi, Rikke Høegh Lorentsen, Mai Faurschou Isaksen, Michael Ward
  • Patent number: 9334488
    Abstract: The present invention relates to eukaryotic cells which have the ability to isomerize xylose directly into xylulose by transformation with nucleotide sequences encoding a xylose isomerase that has one or more specific sequence elements typical for isomerases which are functionally expressed in yeasts, such as xylose isomerases obtainable from bacteria of the genera Clostridium and Fusobacterium or a tunicate from the genus Ciona. The cell preferably is a yeast or a filamentous fungus capable of anaerobic alcoholic fermentation. The cells may further comprise one or more genetic modifications that increase the flux of the pentose phosphate pathway or, reduce unspecific aldose reductase activity. The cell preferably has the ability to produce a fermentation product such as ethanol, lactic acid, 3-hydroxy-propionic acid, ?-lactam antibiotics and cephalosporins. Also provided are processes for producing these fermentation products from xylose.
    Type: Grant
    Filed: December 24, 2009
    Date of Patent: May 10, 2016
    Assignee: DSM IP ASSETS B.V.
    Inventors: Aloysius Wilhelmus Rudolphus Hubertus Teunissen, Johannes Adrianus Maria De Bont
  • Patent number: 9169478
    Abstract: This invention relates generally to enzymes, polynucleotides encoding the enzymes having isomerase activity, e.g., racemase activity, e.g., amino acid racemase activity, alanine racemase activity, and/or epimerase activity, and/or catalyze the re-arrangement of atoms within a molecule, catalyze the conversion of one isomer into another, catalyze the conversion of an optically active substrate into a raceme, which is optically inactive, catalyze the interconversion of substrate enantiomers, catalyze the stereochemical inversion around the asymmetric carbon atom in a substrate having only one center of asymmetry, catalyze the stereochemical inversion of the configuration around an asymmetric carbon atom in a substrate having more than one asymmetric center, and/or catalyze the racemization of amino acids.
    Type: Grant
    Filed: August 14, 2013
    Date of Patent: October 27, 2015
    Assignee: BASF Enzymes LLC
    Inventors: David P. Weiner, Ellen G. Burke, Peter Luginbuhl, Analia Bueno, Joslin M. Cuenca, Mervyn L. De Souza, Sherry Kollmann
  • Publication number: 20150147783
    Abstract: The present invention relates to an isolated mutant eubacterium comprising at least one mutation resulting in a substitution of at least one amino acid in the beta-subunit of the RNA-polymerase encoded for by the rpoB-gene providing an altered production of a product of interest when said production of a product of interest is compared to the production of the same product in an isogenic wild type strain grown at identical conditions, wherein the substitution of at least one amino acid occurs at any of positions 469, 478, 482, 485, or 487 of SEQ ID NO:2, or at the equivalent positions in any eubacterial RNA-polymerase beta-subunit family member. Another aspect of the invention relates to a process for producing at least one product of interest in a mutant eubacterium and to a use of the mutant eubacterium according to the invention for producing at least one product of interest.
    Type: Application
    Filed: February 9, 2015
    Publication date: May 28, 2015
    Inventors: Steen Troels Joergensen, Jens Toenne Andersen, Niels Banke, Preben Nielsen
  • Publication number: 20150147780
    Abstract: Disclosed herein are embodiments for a novel method of producing an organic compound, including harvesting at least one organic compound from an organism or cell line genetically engineered with a gene for at least one proton-pump protein.
    Type: Application
    Filed: November 24, 2014
    Publication date: May 28, 2015
    Inventors: Eugene Dinescu, Vincent Dinescu
  • Publication number: 20150141633
    Abstract: The disclosure relates to a method for enhancing the biosynthesis and/or secretion of sapogenins in the culture medium of plant and microbial cell cultures. Further, the disclosure also relates to the identification of novel genes involved in the biosynthesis of sapogenin intermediates, as well as to novel sapogenin compounds.
    Type: Application
    Filed: May 13, 2013
    Publication date: May 21, 2015
    Inventors: Alain Goossens, Tessa Moses, Jacob Pollier, Lorena Almagro Romero
  • Patent number: 9023629
    Abstract: The present invention relates to host cells transformed with a nucleic acid sequence encoding a eukaryotic xylose isomerase obtainable from an anaerobic fungus. When expressed, the sequence encoding the xylose isomerase confers to the host cell the ability to convert xylose to xylulose which may be further metabolized by the host cell. Thus, the host cell is capable of growth on xylose as carbon source. The host cell preferably is a eukaryotic microorganism such as a yeast or a filamentous fungus. The invention further relates to processes for the production of fermentation products such as ethanol, in which a host cell of the invention uses xylose for growth and for the production of the fermentation product. The invention further relates to nucleic acid sequences encoding eukaryotic xylose isomerases and xylulose kinases as obtainable from anaerobic fungi.
    Type: Grant
    Filed: November 29, 2012
    Date of Patent: May 5, 2015
    Assignee: DSM IP Assets B.V.
    Inventors: Hubertus Johannes Marie Op Den Camp, Harry Ramanoedj Harhangi, Christiaan Van Der Drift, Jacobus Thomas Pronk
  • Publication number: 20150104851
    Abstract: The present invention relates to novel mutants with cyclase activity and use thereof in a method for biocatalytic cyclization of terpenes, such as in particular for the production of isopulegol by cyclization of citronellal; a method for the preparation of menthol and methods for the biocatalytic conversion of further compounds with structural motifs similar to terpene.
    Type: Application
    Filed: December 4, 2014
    Publication date: April 16, 2015
    Inventors: Michael Breuer, Bernhard Hauer, Dieter Jendrossek, Gabrielle Siedenburg, Juergen Pleiss, Demet Sirim, Silvia Racolta
  • Publication number: 20150094209
    Abstract: The present invention provides autonomous replication sequences (ARSs) isolated from Nannochloropsis that support the replication of episomal DNA molecules (EDMs) in eukaryotic cells. The ARSs and EDMs provided herein can be used for expressing genes in organisms including algae and heterokonts.
    Type: Application
    Filed: December 6, 2013
    Publication date: April 2, 2015
    Applicant: Synthetic Genomics, Inc.
    Inventors: Peter DeHoff, Leah Soriaga, Srividya Akella
  • Patent number: 8993301
    Abstract: The present invention relates to novel expression cassettes and expression vectors, comprising three nucleic acid sequences for araA, araB and araD, each coding for a polypeptide of an L-arabinose metabolic pathway, in particular, a bacterial L-arabinose metabolic pathway. The invention particularly relates to expression cassettes and expression vectors, comprising codon-optimized nucleic acid sequences for araA, araB and araD. The invention further relates to host cells, in particular modified yeast strains containing the expression cassettes or expression vectors and expressing the polypeptides for the L-arabinose metabolic pathway, in particular, for the bacterial L-arabinose metabolic pathway. When using these modified host cells, arabinose is more effectively fermented by these cells, in particular into ethanol. The present invention is therefore relevant, inter alia, in connection with the production of biochemicals from biomass, such as bioethanol for example.
    Type: Grant
    Filed: March 31, 2014
    Date of Patent: March 31, 2015
    Assignee: Butalco GmbH
    Inventors: Eckhard Boles, Beate Rother
  • Patent number: 8986948
    Abstract: The present invention relates to the use of nucleic acid molecules coding for a bacterial xylose isomerase (XI), preferably coming from Clostridium phytofermentans, for reaction/metabolization, particularly fermentation, of recombinant microorganisms of biomaterial containing xylose, and particularly for the production of bioalcohols, particularly bioethanol, by means of xylose fermenting yeasts. The present invention further relates to cells, particularly eukaryotic cells, which are transformed utilizing a nucleic acid expression construct which codes for a xylose isomerase, wherein the expression of the nucleic acid expression construct imparts to the cells the capability to directly isomerize xylose into xylulose. Said cells are preferably utilized for reaction/metabolization, particularly fermentation, of biomaterial containing xylose, and particularly for the production of bioalcohols, particularly bioethanol.
    Type: Grant
    Filed: July 1, 2009
    Date of Patent: March 24, 2015
    Assignee: Lesaffre et Compagnie
    Inventors: Dawid Brat, Eckhard Boles, Marco Keller, Beate Wiedemann
  • Publication number: 20150079602
    Abstract: The present invention refers to methods for selectively recognizing a base pair in a DNA sequence by a polypeptide, to modified polypeptides which specifically recognize one or more base pairs in a DNA sequence and, to DNA which is modified so that it can be specifically recognized by a polypeptide and to uses of the polypeptide and DNA in specific DNA targeting as well as to methods of modulating expression of target genes in a cell.
    Type: Application
    Filed: January 13, 2014
    Publication date: March 19, 2015
    Inventors: Ulla Bonas, Jens Boch, Sebastian Schornack, Thomas Lahaye
  • Patent number: 8980594
    Abstract: The invention relates to the use of a protein homologous to a MeaB protein for increasing the enzymatic activity of a 3-hydroxycarboxylic acid-CoA mutase, a fusion protein comprising a 3-hydroxycarboxylic acid-CoA mutase and a protein sequence homologous to a MeaB protein and an enzymatic method for producing 2-hydroxyisobutryric acid.
    Type: Grant
    Filed: October 11, 2010
    Date of Patent: March 17, 2015
    Assignee: Evonik Roehm GmbH
    Inventors: Liv Reinecke, Steffen Schaffer, Tim Koehler, Anja Thiessenhusen, Achim Marx, Markus Buchhaupt
  • Publication number: 20150072380
    Abstract: The present invention relates to the identification of novel nucleic acid sequences, designated herein as 7p, 8k, 7E, 9G, 8Q and 203, in a host cell which effect protein production. The present invention also provides host cells having a mutation or deletion of part or all of the gene encoding 7p, 8k, 7E, 9G, 8Q and 203, which are presented in FIG. 1, and are SEQ ID NOS.: 1-6, respectively. The present invention also provides host cells further comprising a nucleic acid encoding a desired heterologous protein such as an enzyme.
    Type: Application
    Filed: September 23, 2014
    Publication date: March 12, 2015
    Applicant: DANISCO US INC.
    Inventors: Elizabeth A. Bodie, Steve Kim
  • Publication number: 20150037870
    Abstract: The present invention provides enzyme catalysts for Diels-Alder reactions, including intermolecular Diels-Alder reactions, as well as protein scaffolds for making such enzyme catalysts. In other aspects, the invention provides methods of making the enzyme catalysts, including by de novo computational design. The present invention thereby provides enzyme catalysts capable of catalyzing a desired Diels-Alder reaction, including with a specified or desired stereo-selectivity.
    Type: Application
    Filed: February 14, 2014
    Publication date: February 5, 2015
    Applicant: UNIVERSITY OF WASHINGTON
    Inventors: David Baker, Alexandre Zanghellini, Justin Siegel, Jennifer Saint-Clair
  • Patent number: 8936655
    Abstract: The present invention relates to an integrated process, which comprises a single cell oil production process, and a pulp and/or paper industry process. The process comprises that in the single cell oil production process is used a microorganism capable of producing lipids or lipids and enzymes when cultivated on a medium comprising organic material from pulp and/or paper industry. Lipids or lipids and enzymes are produced by said microorganisms in the single cell oil production process and/or in a process connected into it. The present invention relates also to use of lipids produced in the process as biofuel or as a component of biofuel or as a starting material for biofuel production and use of enzymes produced in the lipid production process in pulp and/or paper industry or in other applications as an enzyme preparation or as a source of enzymes.
    Type: Grant
    Filed: December 21, 2011
    Date of Patent: January 20, 2015
    Assignee: Neste Oil Oyj
    Inventors: Perttu Koskinen, Reijo Tanner
  • Patent number: 8932839
    Abstract: The present invention relates to novel mutants with cyclase activity and use thereof in a method for biocatalytic cyclization of terpenes, such as in particular for the production of isopulegol by cyclization of citronellal; a method for the preparation of menthol and methods for the biocatalytic conversion of further compounds with structural motifs similar to terpene.
    Type: Grant
    Filed: November 16, 2011
    Date of Patent: January 13, 2015
    Assignee: BASF SE
    Inventors: Michael Breuer, Bernhard Hauer, Dieter Jendrossek, Gabriele Siedenburg, Juergen Pleiss, Demet Sirim, Silvia Racolta
  • Publication number: 20140377813
    Abstract: The invention provides a microbial eukaryotic cell capable of utilizing C5 sugars, in particular xylose. Another objective of the invention is to provide an improved protein sequence to enable eukaryotic cells to degrade C5 sugars. The present invention thus provides protein comprising an amino acid sequence having at least 75% identity, preferably 80% identity, most preferably 90% identity, most highly preferably 95% identity to SEQ ID NO. 2 or SEQ ID NO. 8 and having xylose-isomerase activity in a eukaryotic cell.
    Type: Application
    Filed: February 7, 2013
    Publication date: December 25, 2014
    Inventors: Zdravko Dragovic, Christian Gamauf, Christoph Reisinger, Ulrich Kettling
  • Publication number: 20140356389
    Abstract: Disclosed herein are various open reading frames from a strain of E. coli responsible for neonatal meningitis (MNEC), and a subset of these that is of particular interest for preparing compositions for immunising against MNEC infections.
    Type: Application
    Filed: June 2, 2014
    Publication date: December 4, 2014
    Applicants: J. CRAIG VENTER INSTITUTE, INC., NOVARTIS VACCINES AND DIAGNOSTICS SRL
    Inventors: Vega MASIGNANI, Danilo Gomes MORIEL, Francesco BERLANDA SCORZA, Nathalie NORAIS, Maria Rita FONTANA, Mariagrazia PIZZA, Laura SERINO, Herve TETTELIN
  • Publication number: 20140335622
    Abstract: Provided are: an expression vector for secreting a protein (Z) to be recovered or a fusion protein having the protein (Z) moiety therein; a method for producing a transformant using the expression vector; the transformant; and a method for producing a protein using the transformant. An expression vector comprising an expression cassette containing a structural gene sequence (y) encoding a protein (Y), a structural gene sequence (z) located downstream from the structural gene sequence (y) and encoding a protein (Z) that is a protein to be recovered, and a promoter sequence and a terminator sequence for expressing a fusion protein containing the protein (Y) moiety and the protein (Z) moiety, characterized in that the protein (Y) is a full-length protein of protein disulfide isomerase 1 (PDI1), a partial protein of PDI1, or a mutant protein of the full-length protein or the partial protein.
    Type: Application
    Filed: July 22, 2014
    Publication date: November 13, 2014
    Applicant: Asahi Glass Company, Limited
    Inventors: Tetsuya KOTANI, Alimujiang Yidirei, Hideki Tohda
  • Patent number: 8871516
    Abstract: The present invention is directed to a method for preparing an expression vector encoding a tailored recombinase, wherein said tailored recombinase recombines asymmetric target sites within the LTR of proviral DNA of a retrovirus inserted into the genome of a host cell and is useful as means for excising the provirus from the genome of the host cell. The present invention further relates to an in vitro-method of optimising the treatment of a retroviral infection of a subject and to the use of tailored recombinases for the preparation of pharmaceutical compositions for reducing the viral load in a subjected infected by a retrovirus.
    Type: Grant
    Filed: January 3, 2008
    Date of Patent: October 28, 2014
    Assignees: Technische Universität Dresden, Max-Planck-Gesellschaft zur Förderung der Wissenschaften E.V., Heinrich-Pette-Institut für Experimentelle Virologie und Immunologie an der Universität Hamburg
    Inventors: Joachim Hauber, Frank Buchholz, Ilona Hauber, Francis A Stewart, Indrani Sarkar
  • Patent number: 8870980
    Abstract: The present invention relates to a process for producing enzymes and single cell oil. The process comprises that microorganisms capable of producing both single cell oil and enzymes are cultivated under conditions suitable for single cell oil production and enzyme production in a single cell oil production process. A microorganism culture comprising single cell oil and enzymes is obtained and at least part of the microorganism culture, of the supernatant and/or microorganism cells separated from the microorganism culture, of protein fraction enriched from the supernatant, and/or of protein fraction obtained from the cells is used as an enzyme preparation or as a source of enzymes. Single cell oil is recovered from the microorganism cells and used as biofuel, component of biofuel or as a starting material for biofuel production. Enzymes produced according to the process are used in the same or in another industrial process.
    Type: Grant
    Filed: December 21, 2011
    Date of Patent: October 28, 2014
    Assignee: Neste Oil Oyj
    Inventors: Annika Malm, Simo Laakso, Ossi Pastinen, Heidi Kahelin, Miia Mujunen
  • Publication number: 20140315313
    Abstract: Described are compositions and methods relating to variant filamentous fungi having altered growth characteristics. Such variants are well-suited for growth in submerged cultures, e.g., for the large-scale production of enzymes and other proteins for commercial applications.
    Type: Application
    Filed: April 20, 2012
    Publication date: October 23, 2014
    Applicant: DANISCO US INC.
    Inventors: Elizabeth A. Bodie, Robert James Pratt, II
  • Publication number: 20140315245
    Abstract: The present disclosure describes methods and systems for improving the expression of a properly folded, biologically active protein of interest in a cell free synthesis system. The methods and systems use a bacterial cell free extract having an active oxidative phosphorylation system, and include an exogenous protein chaperone. The exogenous protein chaperone can be expressed by the bacteria used to prepare the cell free extract. The exogenous protein chaperone can be a protein disulfide isomerase and/or a peptidyl-prolyl cis-trans isomerase. The inventors discovered that the combination of a protein disulfide isomerase and a peptidyl-prolyl cis-trans isomerase produces a synergistic increase in the amount of properly folded, biologically active protein of interest.
    Type: Application
    Filed: April 18, 2014
    Publication date: October 23, 2014
    Applicant: Sutro Biopharma, Inc.
    Inventors: Alice Yam, Dan Groff, Patrick Rivers, Christopher D. Thanos
  • Patent number: 8852891
    Abstract: The present invention relates to a novel N-acetylglucosamine-2-epimerase and a method for preparing CMP-N-acetylneuraminic acid, more specifically, relates to a N-acetylglucosamine-2-epimerase derived from Bacteroides fragilis NCTC 9343, and a method for preparing CMP-N-acetylneuraminic acid using said N-acetylglucosamine-2-epimerase. According to the present invention, CMP-N-acetylneuraminic acid can be produced economically in a large amount through a one-step reaction using cytidine monophosphate and N-acetyl-D-glucosamine which are inexpensive substrates.
    Type: Grant
    Filed: December 7, 2007
    Date of Patent: October 7, 2014
    Assignee: Gene Chem, Inc.
    Inventors: Jin Suk Woo, Jae Kyung Sohng, Byung Gee Kim, Sun Youp Kang, Dea Hee Kim, Kyoung Soon Jang, Ji Young Yang, Young Soo Jung, Won Min Seo, Tae Geon Gil, Sang Hee Shim, In Kang Hur
  • Publication number: 20140295493
    Abstract: The present invention relates to isolated polypeptides having protease activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.
    Type: Application
    Filed: April 1, 2014
    Publication date: October 2, 2014
    Inventors: Hiroaki Udagawa, Christian Isak Jorgensen
  • Patent number: 8846367
    Abstract: Provided is a phenol-producing transformant constructed by transferring a gene which encodes an enzyme having tyrosine phenol-lyase activity into a coryneform bacterium as a host. Also provided is a process for producing phenol, which comprises a step of allowing the transformant to react in a reaction mixture containing tyrosine, a salt thereof, or an ester thereof under reducing conditions, and a step of collecting phenol from the reaction mixture.
    Type: Grant
    Filed: November 17, 2011
    Date of Patent: September 30, 2014
    Inventors: Hideaki Yukawa, Masayuki Inui
  • Publication number: 20140289901
    Abstract: A mutant, non-naturally occurring or transgenic plant cell comprising: (i) a polynucleotide comprising, consisting or consisting essentially of a sequence encoding a neoxanthin synthase and having at least 60% sequence identity to SEQ ID NO:1 or SEQ ID No. 6; (ii) a polypeptide encoded by the polynucleotide set forth in (i); (iii) a polypeptide having at least 66% sequence identity to SEQ ID NO:2 or at least 60% sequence identity to SEQ ID No. 7; or (iv) a construct, vector or expression vector comprising the isolated polynucleotide set forth in (i), and wherein the expression or activity of the neoxanthin synthase is modulated as compared to a control or wild type plant.
    Type: Application
    Filed: October 30, 2012
    Publication date: September 25, 2014
    Inventors: Lucien Bovet, Jeremy Catinot, Joanne Schwaar
  • Publication number: 20140220631
    Abstract: Disclosed are a method for rapid screening of suitable translational fusion partners (TFPs) capable of inducing expression or secretory production of non-producible proteins, which are difficult to produce in conventional recombinant production methods, from a variety of genetic sources, and protein secretion-inducing TFPs obtained using the method.
    Type: Application
    Filed: January 30, 2014
    Publication date: August 7, 2014
    Applicant: KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGY
    Inventors: Jung-Hoon SOHN, Eui-Sung CHOI, Jung-Hoon BAE, Eung-Suck LEE, Mi-Kyung SHIN
  • Publication number: 20140199732
    Abstract: The present invention relates to a D-psicose 3-epimerase variant with improved thermostability by substituting an amino acid at a specific position of an amino acid sequence of a wild type D-psicose 3-epimerase. Further, the present invention provides a recombinant expression vector including a gene of the D-psicose 3-epimerase variant, and a recombinant strain transformed with the recombinant expression vector. Furthermore, the present invention provides an immobilized reactor prepared using the D-psicose 3-epimerase variant or the recombinant strain, and a method of continuously producing D-psicose using the immobilized reactor.
    Type: Application
    Filed: August 21, 2012
    Publication date: July 17, 2014
    Applicant: CJ CHEILJEDANG CORPORATION
    Inventors: Yang Hee Kim, Jin Ha Kim, Young Mi Lee, Young Ho Hong, Min Hae Kim, Seong Bo Kim, Seung Won Park, Seung Hyun Oh, Deok Kun Oh, Jin Geun Choi
  • Patent number: 8778652
    Abstract: The present invention provides recombinant nucleic acid constructs comprising a xylose isomerase polynucleotide, a recombinant fungal host cell comprising a recombinant xylose isomerase polynucleotide, and related methods.
    Type: Grant
    Filed: June 22, 2012
    Date of Patent: July 15, 2014
    Assignee: Codexis, Inc.
    Inventors: Ezhilkani Subbian, John H. Grate, Catherine M. Cho, Benjamin Mijts, Jeanne Bonomo Benoit
  • Publication number: 20140193869
    Abstract: The present disclosure relates, in some aspects, to cell-free methods and systems for large-scale conversion of methane to isobutanol, comprising combining, in a bioreactor at elevated pressure, methane, oxygen, and cell lysates containing methane monooxygenase, methanol dehydrogenase, and enzymes that catalyze the conversion of formaldehyde to isobutanol, to form a cell-free reaction mixture, and incubating under suitable conditions the cell-free reaction to convert methane to isobutanol.
    Type: Application
    Filed: December 20, 2013
    Publication date: July 10, 2014
    Applicant: GreenLight Biosciences, Inc.
    Inventors: William Jeremy Blake, James R. Swartz
  • Patent number: 8772012
    Abstract: It is an object of the disclosure of the present description to provide an eukaryotic cell having xylose utilization ability. The disclosure of the present description provides a novel eukaryotic cell having xylose utilization ability by transforming a yeast or other eukaryotic cell using DNA that codes a xylose isomerase from a termite protist.
    Type: Grant
    Filed: December 22, 2010
    Date of Patent: July 8, 2014
    Assignees: Kabushiki Kaisha Toyota Chuo Kenkyusho, Riken
    Inventors: Satoshi Katahira, Kenro Tokuhiro, Nobuhiko Muramoto, Shigeharu Moriya, Moriya Ohkuma, Yoshina Takahashi
  • Publication number: 20140186884
    Abstract: This disclosure relates to novel xylose isomerases and their uses, particularly in fermentation processes that employ xylose-containing media.
    Type: Application
    Filed: July 23, 2013
    Publication date: July 3, 2014
    Applicant: BP Corporation North Americca Inc.
    Inventors: David Neal Nunn, JR., Peter Luginbuhl, Ling Li, Adam Martin Burja, John Peter Flash Bartnek
  • Publication number: 20140186925
    Abstract: Task: There are provided a highly safe epimerase usable in food industry, and a method for producing a ketose. Resolution: The epimerase is a ketose 3-epimerase obtainable from a microorganism of the genus Arthrobacter, and having (1) substrate specificity whereby a D- or L-ketose is epimerized at position 3 to produce a corresponding D- or L-ketose, and (2) the highest substrate specificity for D-fructose and D-psicose among D- and L-ketoses.
    Type: Application
    Filed: July 5, 2012
    Publication date: July 3, 2014
    Applicants: IZUMORING CO., LTD., MATSUTANI CHEMICAL INDUSTRY CO., LTD.
    Inventors: Ken Izumori, Pushpa Kiran Gullapalli, Tomoya Shintani, Ryo Kikkawa
  • Patent number: 8765443
    Abstract: Disclosed herein are a crystal of a human TOPII (hTOPII)-DNA binary complex, the method for preparing the same and the use thereof. The hTOPII-DNA binary complex includes an hTOPII portion that contains an hTOPII core domain (hTOPIIcore), and a synthetic double-stranded DNA in complex with the hTOPII portion. The synthetic double-stranded DNA has a first DNA strand comprising nucleotide positions 3 to 20 of the sequence of 5?-NNNCCGAGCNNNNGCTCGGNNN-3? (SEQ ID NO: 1), wherein N is any one of adenine, thymine, cytosine, or guanine, and a second DNA strand complementary to the first DNA strand.
    Type: Grant
    Filed: July 24, 2012
    Date of Patent: July 1, 2014
    Assignee: National Taiwan University
    Inventors: Nei-Li Chan, Tsai-Kun Li, Chyuan-Chuan Wu, Ying-Ren Wang
  • Publication number: 20140178933
    Abstract: An expression vector which is capable of overexpressing a protein of interest in a host cell, a host cell comprising the expression vector, and a method of producing a protein of interest are provided.
    Type: Application
    Filed: April 30, 2012
    Publication date: June 26, 2014
    Applicants: RESEARCH & BUSINESS FOUNDATION SUNGKYUNKWAN UNIVERSITY, Samsung Electronics Co., Ltd.
    Inventors: Hyun Min Koo, Byung Jo Yu, Ki Sung Lee, Jae Chan Park, Dae Hyuk Kweon, Sung Minm Park
  • Patent number: 8753862
    Abstract: The present invention relates to novel expression cassettes and expression vectors, comprising three nucleic acid sequences for araA, araB and araD, each coding for a polypeptide of an L-arabinose metabolic pathway, in particular, a bacterial L-arabinose metabolic pathway. The invention particularly relates to expression cassettes and expression vectors, comprising codon-optimised nucleic acid sequences for araA, araB and araD. The invention further relates to host cells, in particular modified yeast strains containing the expression cassettes or expression vectors and expressing the polypeptides for the L-arabinose metabolic pathway, in particular, for the bacterial L-arabinose metabolic pathway. When using these modified host cells, arabinose is more effectively fermented by these cells, in particular into ethanol. The present invention is therefore relevant, inter alia, in connection with the production of biochemicals from biomass, such as bioethanol for example.
    Type: Grant
    Filed: March 20, 2008
    Date of Patent: June 17, 2014
    Assignee: Butalco GmbH
    Inventors: Eckhard Boles, Beate Wiedemann
  • Patent number: 8735106
    Abstract: The present invention relates to a method of successively producing D-psicose from D-fructose or D-glucose by using a psicose-epimerase derived from Agrobacterium tumefaciens which is expressed in a food safety form.
    Type: Grant
    Filed: September 1, 2010
    Date of Patent: May 27, 2014
    Assignee: CJ Cheiljedang Corporation
    Inventors: Young Ho Hong, Jin Ha Kim, Sung Bo Kim, Jung Hoon Kim, Young Mi Lee, Seung Won Park
  • Publication number: 20140141491
    Abstract: This invention relates generally to enzymes, polynucleotides encoding the enzymes having isomerase activity, e.g., racemase activity, e.g., amino acid racemase activity, alanine racemase activity, and/or epimerase activity, and/or catalyze the re-arrangement of atoms within a molecule, catalyze the conversion of one isomer into another, catalyze the conversion of an optically active substrate into a raceme, which is optically inactive, catalyze the interconversion of substrate enantiomers, catalyze the stereochemical inversion around the asymmetric carbon atom in a substrate having only one center of asymmetry, catalyze the stereochemical inversion of the configuration around an asymmetric carbon atom in a substrate having more than one asymmetric center, and/or catalyze the racemization of amino acids.
    Type: Application
    Filed: August 14, 2013
    Publication date: May 22, 2014
    Applicant: Verenium Corporation
    Inventors: David P. WEINER, Ellen G. BURKE, Peter LUGINBUHL, Analia BUENO, Joslin M. CUENCA, Mervyn L. DE SOUZA, Sherry KOLLMANN
  • Patent number: 8679822
    Abstract: Xylose-utilizing Zymomonas strains studied were found to accumulate ribulose when grown in xylose-containing media. Engineering these strains to increase ribose-5-phosphate isomerase activity led to reduced ribulose accumulation, improved growth, improved xylose utilization, and increased ethanol production.
    Type: Grant
    Filed: June 16, 2011
    Date of Patent: March 25, 2014
    Assignee: E I du Pont de Nemours and Company
    Inventors: Perry G. Caimi, Laura McCole, Luan Tao, Jean-Francois Tomb, Paul V. Viitanen
  • Publication number: 20140065679
    Abstract: The present application relates to a mutated Amycoiatopsis sp. TS-1-60 NAAAR that shows improved activity of the enzyme compared with the wild type Amycoiatopsis sp. TS-1-60 NAAAR. The mutated NAAAR is almost five times more active than its wild type counterpart. The present application also relates to the use of mutated Amycoiatopsis sp. TS-1-60 NAAAR in the production of enantiomerically pure amino acid from its N-acyl derivative via dynamic kinetic resolution method.
    Type: Application
    Filed: April 10, 2012
    Publication date: March 6, 2014
    Applicants: THE UNIVERSITY OF EDINBURGH, DR. REDDY'S LABORATORIES (EU) LIMITED
    Inventors: Scott Baxter, Dominic Campopiano, Karen Elizabeth Holt-Tiffin
  • Patent number: 8658160
    Abstract: A composition including an effective amount of a treatment agent to inhibit tumor growth in a mammal by manipulating a prostaglandin D2 biosynthetic pathway. A method comprising introducing an effective amount of a treatment agent to a mammal to inhibit tumor growth by manipulating a prostaglandin D2 biosynthetic pathway while at the same time reducing production of other forms of prostaglandins that may produce ill effects, such as prostaglandin E2. A method comprising introducing an effective amount of a treatment agent to a mammal to inhibit tumor growth by manipulating a prostaglandin D2 biosynthetic pathway while at the same time reducing potential undesirable side effects related to increased levels of prostaglandin D2. A composition comprising a genetic variant of human hematopoietic prostaglandin D synthase (H-PGDS). A method comprising identifying an individual who carries a Val187Ile gene variant or allele and assessing a predisposition of an individual to various conditions.
    Type: Grant
    Filed: October 16, 2007
    Date of Patent: February 25, 2014
    Assignee: Los Angeles Biomedical Research Institute at Harbor-UCLA Medical Center
    Inventors: Henry John Lin, Brigette L. Tippin
  • Publication number: 20140017765
    Abstract: The present invention provides recombinant nucleic acid constructs comprising a xylose isomerase polynucleotide, a recombinant fungal host cell comprising a recombinant xylose isomerase polynucleotide, and related methods.
    Type: Application
    Filed: September 24, 2013
    Publication date: January 16, 2014
    Applicant: Codexis, Inc.
    Inventors: Ezhilkani Subbian, Xiyun Zhang, Benjamin N. Mijts, Catherine M. Cho
  • Publication number: 20130340120
    Abstract: Provided are a method for enhancing yield-related traits in plants by modulating expression in a plant of a nucleic acid encoding a DUF642 (Protein containing a Domain of Unknown Function) polypeptide, or an epimerase-related like polypeptide, or a phospholipase/carboxylesterase (PLPCase) polypeptide, and plants having modulated expression of a nucleic acid encoding a DUF642 polypeptide, or an epimerase-related like polypeptide, or a PLPCase polypeptide, which plants have enhanced yield-related traits relative to control plants. Also provided are DUF642-encoding nucleic acids, or epimerase-related like polypeptides, or PLPCase-encoding nucleic acids, and constructs comprising the same, useful in performing the methods for enhancing yield-related traits in plants.
    Type: Application
    Filed: February 27, 2012
    Publication date: December 19, 2013
    Applicant: BASF PLANT SCIENCE COMPANY GMBH
    Inventors: Marieke Louwers, Christophe Reuzeau, Ana Isabel Sanz Molinero, Yves Hatzfeld