Glucose Isomerase Patents (Class 435/234)
  • Patent number: 9017953
    Abstract: The present invention encompasses methods and compositions for detecting pathogenic bacteria. Additionally, the present invention encompasses methods and compositions for catalyzing the dismutation of superoxide radicals.
    Type: Grant
    Filed: May 20, 2013
    Date of Patent: April 28, 2015
    Assignee: Washington University
    Inventors: Jeffrey P. Henderson, Chia Hung, Kaveri Chaturvedi
  • Publication number: 20150099276
    Abstract: The present invention relates to the use of nucleic acid molecules coding for a bacterial xylose isomerase (XI), preferably coming from Clostridium phytofermentans, for reaction/metabolization, particularly fermentation, of recombinant microorganisms of biomaterial containing xylose, and particularly for the production of bioalcohols, particularly bioethanol, by means of xylose fermenting yeasts. The present invention further relates to cells, particularly eukaryotic cells, which are transformed utilizing a nucleic acid expression construct which codes for a xylose isomerase, wherein the expression of the nucleic acid expression construct imparts to the cells the capability to directly isomerize xylose into xylulose. Said cells are preferably utilized for reaction/metabolization, particularly fermentation, of biomaterial containing xylose, and particularly for the production of bioalcohols, particularly bioethanol.
    Type: Application
    Filed: December 16, 2014
    Publication date: April 9, 2015
    Inventors: Dawid BRAT, Eckhard BOLES, Marco KELLER, Beate ROTHER
  • Publication number: 20150037868
    Abstract: Various aspects and embodiments herein relate to recombinant proteins with at least one protease recognition sequence that can be inactivated by a cognate protease and methods of preparing such proteins. In some embodiments, recombinant phosphoglucose isomerase (Pgi) proteins are provided. In other embodiments, recombinant phosphotransacetylase (Pta) proteins are provided. In yet other embodiments, recombinant transketolase A (TktA) proteins are provided.
    Type: Application
    Filed: August 5, 2014
    Publication date: February 5, 2015
    Applicant: GreenLight Biosciences, Inc.
    Inventors: William Jeremy Blake, Drew S. Cunningham
  • Publication number: 20140377800
    Abstract: The present invention relates to methods of optimization of a protein coding sequences for expression in a given host cell. The methods apply genetic algorithms to optimise single codon fitness and/or codon pair fitness sequences coding for a predetermined amino acid sequence. In the algorithm generation of new sequence variants and subsequent selection of fitter variants is reiterated until the variant coding sequences reach a minimum value for single codon fitness and/or codon pair fitness. The invention also relates to a computer comprising a processor and memory, the processor being arranged to read from and write into the memory, the memory comprising data and instructions arranged to provide the processor with the capacity to perform the genetic algorithms for optimisation of single codon fitness and/or codon pair fitness.
    Type: Application
    Filed: May 20, 2014
    Publication date: December 25, 2014
    Inventors: Johannes Andries ROUBOS, Noel Nicolaas Maria Elisabeth VAN PEIJ
  • Patent number: 8802393
    Abstract: The present invention relates to a thermophilic arabinose isomerase and a method of manufacturing tagatose using the same, and more precisely, a gene encoding arabinose isomerase originating from the thermophile Thermotoga neapolitana DSM 5068, a recombinant expression vector containing the gene, a method of preparing a food grade thermophilic arabinose isomerase from the recombinant GRAS (Generally Recognized As Safe) strain transformed with the said expression vector, and a method of preparing tagatose from galactose using the said enzyme.
    Type: Grant
    Filed: October 17, 2008
    Date of Patent: August 12, 2014
    Assignee: CJ Cheiljedang Corporation
    Inventors: Seong-bo Kim, Young-mi Lee, Seung-won Park, Jung-hoon Kim, Sang-hoon Song, Kang-pyo Lee, Hye-won Kim, Hye-jin Choi
  • Patent number: 8748152
    Abstract: A xylose isomerase (XI) enzyme which exhibits increased activity and affinity for xylose is produced by strain TC2-24 of the rumen bacterium, Prevotella ruminicola. The gene encoding this enzyme may be used to produce improved recombinant yeast capable of utilizing xylose. The recombinant yeast are preferably transformed with heterologous polynucleotide sequences coding both the P. ruminicola XI, and the xylulokinase (XKS) of a Prevotella species. Yeast transformed with the polynucleotide sequences coding both of these XI and XKS exhibit significantly increased xylose utilization and cell growth on a culture medium containing xylose as the sole carbon source, in comparison to yeast transformed with XKS and XI from other sources.
    Type: Grant
    Filed: September 14, 2012
    Date of Patent: June 10, 2014
    Assignee: The United States of America, as represented by the Secretary of Agriculture
    Inventors: Ronald E. Hector, Bruce S. Dien, Michael A. Cotta
  • Publication number: 20130302411
    Abstract: There is provided in accordance with embodiments of the invention a method of treating or reducing the effects in a subject of a condition selected from fructose intolerance and impaired fructose metabolism, the method comprising administering to a subject in need of such treatment or reduction an efficacious amount of a glucose isomerase, other than in combination with 5-D-fructose dehydrogenase. Other embodiments are also disclosed. There is provided a method for treating or reducing the effects of fructose intolerance and health problems associated with excessive fructose intake by administration of glucose isomerase. Other embodiments are also disclosed.
    Type: Application
    Filed: March 15, 2013
    Publication date: November 14, 2013
    Inventors: Daniel Henry WYROBNIK, Isaac Harry Wyrobnik
  • Publication number: 20130202695
    Abstract: There is provided in accordance with embodiments of the invention a method of treating or reducing the effects in a subject of a condition selected from fructose intolerance and impaired fructose metabolism, the method comprising administering to a subject in need of such treatment or reduction an efficacious amount of a glucose isomerase, other than in combination with 5-D-fructose dehydrogenase. Other embodiments are also disclosed.
    Type: Application
    Filed: March 15, 2013
    Publication date: August 8, 2013
    Inventors: Daniel Henry WYROBNIK, Isaac Harry Wyrobnik
  • Patent number: 8460911
    Abstract: There is provided in accordance with embodiments of the invention a method of treating or reducing the effects in a subject of a condition selected from fructose intolerance and impaired fructose metabolism, the method comprising administering to a subject in need of such treatment or reduction an efficacious amount of a glucose isomerase, other than in combination with 5-D-fructose dehydrogenase. Other embodiments are also disclosed.
    Type: Grant
    Filed: August 20, 2009
    Date of Patent: June 11, 2013
    Assignee: Vitacare GmbH & Co. KG
    Inventors: Daniel Henry Wyrobnik, Isaac Harry Wyrobnik
  • Publication number: 20120252073
    Abstract: A method of decomposing stubble, which is crop residue left on the ground, and a composition for treating the stubble are disclosed. The method comprises treating the stubble with the composition comprising a) polysaccharide-degrading enzyme; b) wetting agent; and, optionally, c) water and allowing the treated stubble to remain on the ground and decompose.
    Type: Application
    Filed: March 28, 2012
    Publication date: October 4, 2012
    Applicant: RHODIA OPERATIONS
    Inventors: Jean-Christophe Castaing, Gary Groves, Ashwin Rao, Thomas E. Ruch
  • Publication number: 20120231504
    Abstract: A septum is positioned within a disposable vessel and defines a lower chamber and an upper chamber. The septum includes a plurality of apertures that provide fluid communication between the upper chamber and lower chamber. Compressed gas is introduced in the lower chamber to produce fine bubbles rising up throughout the vessel to produce a mixing and gasification needed for the growth of a biological culture and manufacture of a biological product in a nutrient medium. Adding a binding resin to the upper chamber allows harvesting, separation and purification of biological products in the reactor as a single unit operation.
    Type: Application
    Filed: September 3, 2011
    Publication date: September 13, 2012
    Applicant: Therapeutic Proteins Inc.
    Inventor: Sarfaraz K. Niazi
  • Publication number: 20120142047
    Abstract: An assembly capable of capturing and purifying expressed biological products during or at the end of a bioreaction cycle is disclosed wherein a binding resin is kept separated from the contents of the bioreactor allowing capturing, harvesting and purification of biological products in a bioreactor; the invention additionally provides means of removing undesirable metabolic products as well as provides for efficient loading of chromatography columns.
    Type: Application
    Filed: November 29, 2011
    Publication date: June 7, 2012
    Applicant: Therapeutic Proteins, Inc.
    Inventor: Sarfaraz K. Niazi
  • Patent number: 8153413
    Abstract: The invention aims to provide a novel alkaline protease having peculiar properties such as high alkali activity, resistance to surfactants and calcium-dependent thermostability and exhibiting excellent performance in highly alkaline detergents, and a gene coding for the amino acid sequence thereof. There is provided an alkaline protease with such properties that an active pH range is from 5 to 13, an optimum pH is approximately 12.6, an optimum temperature is 70° C., no activity drop by heating is observed up to 65° C. at pH 10 and the optimum temperature and the thermostability are not affected by Ca2+ ions. Specifically, there is provided, for example, an alkaline protease having an amino acid sequence constituting a mature enzyme as represented by SEQ ID NO: 3 or an amino acid sequence resulting from deletion, substitution, situs inversus arrangement, addition or insertion of a part of amino acids thereof, or derived from Alkaliphillus transvaalensis.
    Type: Grant
    Filed: November 16, 2005
    Date of Patent: April 10, 2012
    Assignee: Japan Agency for Marine-Earth Science and Technology
    Inventors: Jie Lu, Zhi jun Li, Si Hung Vo, Yuji Hatada, Ken Takai, Susumu Ito, Koki Horikoshi
  • Patent number: 8067561
    Abstract: This invention provides a series of recombinant Thermoanaerobacterium saccharolyticum glucose isomerases with improved catalytic activity and thermostability obtained by using recombinant techniques. These recombinant glucose isomerases comprise amino acid variation including phenylalanine (Phe) at position 139, alanine (Ala) at position 182, serine (Ser) at position 187, and glutamine (Gin) at position 299, and carry at least one additional mutated amino acid at position 87, position 217, position 260 or position 276, and possess a higher catalytic activity than that of the wild-type when using D-glucose as substrate. These recombinant glucose isomerases can be used for direct production of high fructose corn syrup containing 55% [wt] or higher concentration of fructose.
    Type: Grant
    Filed: March 15, 2010
    Date of Patent: November 29, 2011
    Assignee: BioRight Worldwide Company Limited
    Inventors: Jun Wang, Caike Jin, Rongzhao Fu, Dong Shen
  • Patent number: 8048649
    Abstract: Methods and compositions for increased production of amino acids from C. glutamicum using sucrose as a carbon source are described. In one aspect, increased production of L-lysine from C. glutamicum is accomplished by using a strain having a mutation in the ptsF gene encoding fructose-PTS enzyme that attenuates or blocks fructose import into the cell when such strain is grown on media containing sucrose as a carbon source and production is increased by providing glucose isomerase in the fermentation media. The glucose isomerase may be exogenously added or expressed in the strain and exported into the media. In certain embodiments the media also contain an invertase. In another aspect increased production of L-lysine is accomplished by making a C. glutamicum strain having the ptsF mutation and a second mutation in a fructose exporter function. The dual mutation retains imported fructose in the cell.
    Type: Grant
    Filed: September 26, 2008
    Date of Patent: November 1, 2011
    Assignee: Archer Daniels Midland Company
    Inventors: Thomas P. Binder, Paul D. Hanke
  • Patent number: 7923222
    Abstract: This invention provides a series of recombinant Thermoanaerobacterium saccharolyticum glucose isomerases with improved catalytic activity and thermostability obtained by using recombinant techniques. These recombinant glucose isomerases comprise amino acid variation including phenylalanine (Phe) at position 139, alanine (Ala) at position 182, serine (Ser) at position 187, and glutamine (Gin) at position 299, and carry at least one additional mutated amino acid at position 87, position 217, position 260 or position 276, and possess a higher catalytic activity than that of the wild-type when using D-glucose as substrate. These recombinant glucose isomerases can be used for direct production of high fructose corn syrup containing 55% [wt] or higher concentration of fructose.
    Type: Grant
    Filed: March 15, 2010
    Date of Patent: April 12, 2011
    Assignee: Geneharbor (HK) Technologies Ltd.
    Inventors: Jun Wang, Caike Jin, Rongzhao Fu, Dong Shen
  • Patent number: 7919300
    Abstract: This invention describes a series of recombinant Thermoanaerobacterium saccharolyticum glucose isomerases having improved catalytic activity and thermostability. The recombinant glucose isomerases can be used for direct production of fructose syrup containing 55 wt % or higher concentration of fructose, or used for the production of fructose syrup containing less than 55 wt % fructose.
    Type: Grant
    Filed: July 28, 2004
    Date of Patent: April 5, 2011
    Inventors: Jun Wang, Rongzhao Fu, Dong Shen, Caike Jin, Zhangming Liu, Junming Chen
  • Publication number: 20100233783
    Abstract: Process for purifying a recombinant protein including one or a few procedural steps only. The process combines the step of lysis of the host cell, with the purification of the protein of interest, allowing for a rapid and much more efficient process of purification. The conditions used during the purification process are those of a high temperature and a low pH, allowing for thermostable and acid-resistant recombinant proteins to be isolated from a suspension. The invention also relates to purifying recombinant proteins which are fusion proteins, wherein one part of the protein may be selected from an enamel matrix protein, such as amelogenin.
    Type: Application
    Filed: March 23, 2006
    Publication date: September 16, 2010
    Applicant: Straumann Holding AG
    Inventors: Johan Per Henrik Svensson, Leif Bülow
  • Publication number: 20100234581
    Abstract: The present invention relates to a isolated polypeptide characterised in that it comprises an amino acid sequence having at least 80% identity to a sequence selected from the group consisting of SEQ ID NO 2, SEQ ID NO 21 and SEQ ID NO 22, to polynucleotides encoding such a polypeptide and to the use thereof in the production of fructose syrups.
    Type: Application
    Filed: September 6, 2006
    Publication date: September 16, 2010
    Inventors: Luigi Concilio, Tiziana Giovannini, Claudio Merendi
  • Patent number: 7704719
    Abstract: This invention provides a series of recombinant Thermoanaerobacterium saccharolyticum glucose isomerases with improved catalytic activity and thermostability obtained by using recombinant techniques. These recombinant glucose isomerases comprise amino acid variation including phenylalanine (Phe) at position 139, alanine (Ala) at position 182, serine (Ser) at position 187, and glutamine (Gln) at position 299, and carry at least one additional mutated amino acid at position 87, position 217, position 260 or position 276, and possess a higher catalytic activity than that of the wild-type when using D-glucose as substrate. These recombinant glucose isomerases can be used for direct production of high fructose corn syrup containing 55.% [wt] or higher concentration of fructose.
    Type: Grant
    Filed: October 30, 2006
    Date of Patent: April 27, 2010
    Assignee: GeneHarbor (HK) Technologies Ltd.
    Inventors: Jun Wang, Caike Jin, Rongzhao Fu, Dong Shen
  • Publication number: 20090311232
    Abstract: There is provided in accordance with embodiments of the invention a method of treating or reducing the effects in a subject of a condition selected from fructose intolerance and impaired fructose metabolism, the method comprising administering to a subject in need of such treatment or reduction an efficacious amount of a glucose isomerase, other than in combination with 5-D-fructose dehydrogenase. Other embodiments are also disclosed.
    Type: Application
    Filed: August 20, 2009
    Publication date: December 17, 2009
    Inventors: Daniel Henry Wyrobnik, Isaac Harry Wyrobnik
  • Patent number: 7605245
    Abstract: The invention generally relates to polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) systems isolated from or derived from non-bacterial organisms, to homologues thereof, to isolated nucleic acid molecules and recombinant nucleic acid molecules encoding biologically active domains of such a PUFA PKS system, to genetically modified organisms comprising PUFA PKS systems, to methods of making and using such systems for the production of bioactive molecules of interest, and to novel methods for identifying new bacterial and non-bacterial microorganisms having such a PUFA PKS system.
    Type: Grant
    Filed: July 12, 2007
    Date of Patent: October 20, 2009
    Assignee: Martek Biosciences Corporation
    Inventors: James G. Metz, James H. Flatt, Jerry M. Kuner, William R. Barclay
  • Publication number: 20080305529
    Abstract: This invention provides a series of recombinant Thermoanaerobacterium saccharolyticum glucose isomerases with improved catalytic activity and thermostability obtained by using recombinant techniques. These recombinant glucose isomerases comprise amino acid variation including phenylalanine Phe) at position 139, alanine (Ala) at position 182, serine (Ser) at position 187, and glutamine (Gln) at position 299, and carry at least one additional mutated amino acid at position 87, position 217, position 260 or position 276, and possess a higher catalytic activity than that of the wild-type when using D-glucose as substrate. These recombinant glucose isomerases can be used for direct production of high fructose corn syrup containing 55.% [wt] or higher concentration of fructose.
    Type: Application
    Filed: October 30, 2006
    Publication date: December 11, 2008
    Applicant: GENEHARBOR (HK) TECHNOLOGIES LTD.
    Inventors: Jun Wang, Caike Jin, Rongzhao Fu, Dong Shen
  • Publication number: 20080124770
    Abstract: The present invention relates to a thermophilic arabinose isomerase and a method of manufacturing tagatose using the same, and more precisely, a gene encoding arabinose isomerase originating from the thermophilic Thermotoga neapolitana DSM 5068, a recombinant expression vector containing the gene, a method of preparing a food grade thermophilic arabinose isomerase from the recombinant GRAS (Generally Recognized As Safe) strain transformed with the said expression vector, and a method of preparing tagatose from galactose using the said enzyme.
    Type: Application
    Filed: November 30, 2006
    Publication date: May 29, 2008
    Inventors: Seong-bo Kim, Young-mi Lee, Seung-won Park, Jung-hoon Kim, Sang-hoon Song, Kang-pyo Lee, Hye-won Kim, Hye-jin Choi
  • Publication number: 20080113415
    Abstract: This invention describes a series of recombinant Thermoanaerobacterium saccharolyticum glucose isomerases having improved catalytic activity and thermostability. The recombinant glucose isomerases can be used for direct production of fructose syrup containing 55 wt % or higher concentration of fructose, or used for the production of fructose syrup containing less than 55 wt % fructose.
    Type: Application
    Filed: July 28, 2004
    Publication date: May 15, 2008
    Inventors: Jun Wang, Pongzhao Fu, Dorg Shen, Caike Jin, Zhangming Liu, Junming Chen
  • Patent number: 7125695
    Abstract: The present invention provides methods for directing the evolution of microorganisms comprising the use of mutator genes and growth under conditions of selective pressure. The method discloses mutator genes which can be used in the methods of the present invention and provides ATCC deposits which exemplify the evolved microorganisms produced by the methods.
    Type: Grant
    Filed: November 20, 2003
    Date of Patent: October 24, 2006
    Assignee: Genencor International, Inc.
    Inventors: Volker Schellenberger, Amy D. Liu, Olga V. Selifonova
  • Publication number: 20040235099
    Abstract: This invention provides a series of low-copy number plasmids comprising restriction endonuclease recognition sites useful for cloning at least three different genes or operons, each flanked by a terminator sequence, the plasmids containing variants of glucose isomerase promoters for varying levels of protein expression. The materials and methods are useful for genetic engineering in microorganisms, especially where multiple genetic insertions are sought.
    Type: Application
    Filed: December 18, 2003
    Publication date: November 25, 2004
    Inventors: Mark S. Payne, Stephen K. Picataggio, Amy Kuang-Hua Hsu, Ramesh V. Nair, Fernando Valle, Philippe Soucaille, Donald E. Trimbur
  • Publication number: 20040197894
    Abstract: The present invention relates to novel subtilase variants exhibiting alterations relative to the parent subtilase in one or more properties including: Wash performance, thermal stability, storage stability or catalytic activity. The variants of the invention are suitable for use in e.g. cleaning or detergent compositions, such as laundry detergent compositions and dishwash compositions, including automatic dishwash compositions.
    Type: Application
    Filed: December 22, 2003
    Publication date: October 7, 2004
    Inventors: Tina Sejersgard Fano, Claus Von Der Osten, Malene Kappen Kruger, Mads Norregaard-Madsen
  • Patent number: 6743619
    Abstract: The present invention provides novel nucleic acids, novel polypeptide sequences encoded by these nucleic acids and uses thereof.
    Type: Grant
    Filed: January 30, 2001
    Date of Patent: June 1, 2004
    Assignee: Nuvelo
    Inventors: Y. Tom Tang, Ping Zhou, Ryle Goodrich, Chenghua Liu, Vinod Asundi, Feiyan Ren, Jie Zhang, Qing A. Zhao, Yonghong Yang, Aidong J. Xue, Tom Wehrman, Jian-Rui Wang, Dunrui Wang, Radoje T. Drmanac
  • Patent number: 6706503
    Abstract: The present invention provides methods for directing the evolution of microorganisms comprising the use of mutator genes and growth under conditions of selective pressure. The method discloses mutator genes which can be used in the methods of the present invention and provides ATCC deposits which exemplify the evolved microorganisms produced by the methods.
    Type: Grant
    Filed: October 23, 2001
    Date of Patent: March 16, 2004
    Assignee: Genencor International, Inc.
    Inventors: Volker Schellenberger, Amy D. Liu, Olga V. Selifonova
  • Publication number: 20040038372
    Abstract: The invention relates to polynucleotide sequences of pgi genes encoding polypeptide sequences having phosphoglucose isomerase activity from coryneform bacteria, to coryneform bacteria containing such polynucleotides and/or polypeptides, a process for the production of L-amino acids using such polynucleotides and/or polypeptides, and methods of screening and amplifying polynucleotides encoding polypeptide sequences which comprise varying degrees of phosphoglucose isomerase activity.
    Type: Application
    Filed: May 8, 2003
    Publication date: February 26, 2004
    Applicant: DEGUSSA AG
    Inventors: Brigitte Bathe, Achim Marx, Walter Pfefferle, Georg Thierbach
  • Patent number: 6680190
    Abstract: The present invention relates, in general, to a method of producing L-amino acids comprising culturing altered bacterial cells having increased amounts of NADPH as compared to unaltered bacterial cells whereby L-amino acids yields from said altered bacterial cells are greater than yields from unaltered bacterial cells. The invention also relates to a gene encoding phosphoglucoisomerase.
    Type: Grant
    Filed: August 20, 2002
    Date of Patent: January 20, 2004
    Assignee: Archer-Daniels-Midland Company
    Inventor: Paul D. Hanke
  • Publication number: 20030166172
    Abstract: The present invention provides novel isolated polynucleotides encoding a novel xylose isomerases capable of catalyzing the conversion of glucose to fructose and nucleic acids encoding for such isomerase. The present invention also provides a method of producing the xylose isomerase enzymes employing DNA encoding for the enzymes, plasmids containing the DNA, and bacteria into which the plasmids have been inserted and which produce the enzymes.
    Type: Application
    Filed: January 21, 2003
    Publication date: September 4, 2003
    Applicant: Board of Trustees of MICHIGAN STATE UNIVERSITY
    Inventors: J. Gregory Zeikus, Dinlaka Sriprapundh, Claire Vieille
  • Patent number: 6465238
    Abstract: The present invention relates, in general, to a method of producing L-amino acids comprising culturing altered bacterial cells having increased amounts of NADPH as compared to unaltered bacterial cells whereby L-amino acids yields from said altered bacterial cells are greater than yields from unaltered bacterial cells. The invention also relates to a gene encoding phosphoglucoisomerase.
    Type: Grant
    Filed: July 21, 2000
    Date of Patent: October 15, 2002
    Assignee: Archer-Daniels-Midland Company
    Inventor: Paul D. Hanke
  • Patent number: 6410012
    Abstract: This invention pertains in part to a method for delivering functional DNA or antigens. The desired DNA is introduced into attenuated bacteria able to enter cells. Once the bacteria is in the cell, antimicrobial agents are introduced such that they enter the mammalian cell and lyse the bacteria thereby allowing the delivery of carried functional DNA or antigens. The advantages of this method and its uses are described.
    Type: Grant
    Filed: March 27, 1998
    Date of Patent: June 25, 2002
    Assignee: The United States of America as represented by the Secretary of the Army
    Inventors: Donata R. Sizemore, Jerald C. Sadoff, Jason C. Grove
  • Patent number: 6171856
    Abstract: The present invention relates to methods and compositions for the treatment of diabetes involving free radicals. In particular, the present invention is directed to the treatment or prophylactic intervention of diabetes. The present invention demonstrates that MnSOD can play a protective role against cytokine killing, and provides strategies for engineering cell lines as islet surrogates for transplantation therapy of diabetes mellitus. Further, the present invention shows that &bgr;-cell destruction and dysfunction in adipogenic diabetes is mediated via fatty acids. Methods and compositions for ameliorating this disorder are provided herein.
    Type: Grant
    Filed: July 30, 1998
    Date of Patent: January 9, 2001
    Assignees: Board of Regents, The University of Texas System, Betagene, Inc
    Inventors: Anice Thigpen, Hans-Ewald Hohmeier, Christopher B. Newgard, Roger H. Unger, Michio Shimabukuro, Guoxun Chen, Christopher J. Rhodes, Sigrun R. H{umlaut over (u)}gl, Sharon Cousin
  • Patent number: 5945278
    Abstract: The invention provides a method and expression system for enhancing secretion of hyperproduced homologous and heterologous exoproteins in gram-positive bacteria such as Bacillus sp. The method and system comprise overproduction of PrsA protein in a gram-positive bacterial host also overproducing at least one exoprotein of interest. Use of the method and system of the invention results in greatly enhanced secretion of the synthesized exoproteins into the growth medium. Once in the growth medium these secreted exoproteins can be recovered and purified in a straightforward manner.
    Type: Grant
    Filed: July 1, 1998
    Date of Patent: August 31, 1999
    Assignee: The Finnish National Public Health Institute
    Inventors: Vesa Kontinen, Matti Sarvas
  • Patent number: 5900364
    Abstract: The invention relates to a novel water-insoluble glucose isomerase which is formed by a crystalline enzyme converted to solid form by cross-linking. The invention also concerns a process for the preparation of the novel crystalline glucose isomerase by cross-linking with dialdehyde in the presence of a compound containing at least one amino group, and the use of this novel enzyme preparation as an isomerization catalyst.
    Type: Grant
    Filed: April 19, 1995
    Date of Patent: May 4, 1999
    Assignee: Genencor International, Inc.
    Inventor: Kalevi Visuri
  • Patent number: 5811280
    Abstract: The invention relates to a novel water-insoluble glucose isomerase which is formed by a crystalline enzyme converted to solid form by cross-linking. The invention also concerns a process for the preparation of the novel crystalline glucose isomerase by cross-linking with dialdehyde in the presence of a compound containing at least one amino group, and the use of this novel enzyme preparation as an isomerization catalyst.
    Type: Grant
    Filed: April 19, 1995
    Date of Patent: September 22, 1998
    Assignee: Genencor International, Inc.
    Inventor: Kalevi Visuri
  • Patent number: 5710032
    Abstract: Secondary-metabolite biosynthesis genes from actinomycetes, method of isolating them, and their use.The invention concerns secondary-metabolite biosynthesis genes from actinomycetes, a method of isolating secondary-metabolite and, in particular, 6-deoxy-sugar bio-synthesis genes, from actinomycetes using the gene probes strD, strE, strL and strM gene probes from Streptomyces griseus DSM40236, and structurally related genes, as gene probes for detecting the genes snot (coding for amphotheronolide B-dTDP-D-mycosaminyl transferase), snoD (coding for dTDP-D-glucose synthase) and snoM (coding for dTDP-4-keto-6-deoxy-D-glucose isomerase), or one or more secondary-metabolite biosynthesis genes from actinomycetes. The invention also concerns the use of secondary-metabolite biosynthesis genes thus isolated.
    Type: Grant
    Filed: July 30, 1996
    Date of Patent: January 20, 1998
    Assignee: Hoechst Aktiengesellschaft
    Inventors: Wolfgang Piepersberg, Barbara Brau, Petra Sichel
  • Patent number: 5656497
    Abstract: The present invention describes the cloning and expression of a hyperthermostable xylose isomerase gene in an industrial host and the heat facilitated purification of the enzyme. The hyperthermostable enzyme has unique features sought by the fructose syrup industry.
    Type: Grant
    Filed: November 15, 1995
    Date of Patent: August 12, 1997
    Assignee: Michigan State University
    Inventors: Joseph Gregory Zeikus, Claire Vieille
  • Patent number: 5593868
    Abstract: A process for producing high fructose corn syrup from glucose containing syrup, which entails isomerizing the glucose containing syrup through an enzymatic reaction to make high fructose corn syrup, wherein the enzymatic reaction occurs in a gas containing solution which comprises at least one gas selected from the group consisting of noble gas.
    Type: Grant
    Filed: February 6, 1996
    Date of Patent: January 14, 1997
    Assignee: American Air Liquide
    Inventors: Kevin C. Spencer, Christine E. Boisrobert, Steven A. Fisher, Patricia A. Rojak, Karen S. Sabatini
  • Patent number: 5543576
    Abstract: A method of catalyzing in vitro reactions using seeds containing enhanced amounts of enzymes is disclosed. The method involves adding transgenic, non-wild type seeds, preferably in a ground form, to a reaction mixture and allowing the enzymes in the seeds to increase the rate of reaction. By directly adding the seeds to the reaction mixture the method provides a solution to the expensive and problematic process of extracting and purifying the enzyme. Methods of treatment are also provided whereby a subject lacking a sufficient supply of an enzyme is administered the enzyme in the form of seeds containing enhanced amounts of the enzyme.
    Type: Grant
    Filed: November 2, 1993
    Date of Patent: August 6, 1996
    Assignees: Mogen International, Gist-Brocades
    Inventors: Albert J. J. van Ooijen, Krijn Rietveld, Andreas Hoekema, Jan Pen, Peter C. Sijmons, Teunis C. Verwoerd, Wilhemus J. Quax
  • Patent number: 5512464
    Abstract: A process for producing high fructose corn syrup from glucose containing syrup, which entails isomerizing the glucose containing syrup through an enzymatic reaction to make high fructose corn syrup, wherein the enzymatic reaction occurs in a gas containing solution which comprises at least one gas selected from the group consisting of noble gas.
    Type: Grant
    Filed: July 1, 1994
    Date of Patent: April 30, 1996
    Assignee: American Air Liquide
    Inventors: Kevin C. Spencer, Christine E. Boisrobert, Steven A. Fisher, Patricia A. Rojak, Karen S. Sabatini
  • Patent number: 5437993
    Abstract: The invention relates to a novel water-insoluble glucose isomerase which is formed by a crystalline enzyme converted to solid form by cross-linking. The invention also concerns a process for the preparation of the novel crystalline glucose isomerase by cross-linking with dialdehyde in the presence of a compound containing at least one amino group, and the use of this novel enzyme preparation as an isomerization catalyst. Preferrably the compound having an amino group is an ammonium salt, lysine or tryptophan and the dialdehyde is glutaraldehyde.
    Type: Grant
    Filed: November 8, 1993
    Date of Patent: August 1, 1995
    Assignee: Strabra AG
    Inventor: Kalevi Visuri
  • Patent number: 5411886
    Abstract: A xylose isomerase gene from Thermus bacteria, such as Thermos aquaticus (ATCC 27634) and a gene having 60% or more of homology to the nucleotide sequence of Thermus aquaticus xylose isomerase gene of FIG. 1-3. A xylose isomerase from Thermus aquaticus characterized in that the xylose isomerase has the optimal pH of about 7, the stable pH range of from about 6 to 8.5, the optimal temperature of about 95.degree. C. and the molecular weight of about 44,000, and is stabilized with manganese or magnesium. A process for preparing a xylose isomerase comprising transforming a microorganism with a plasmid containing the above gene and a promoter, culturing the transformed microorganism and harvesting the produced xylose isomerase. A process for preparing fructose comprising isomerization of glucose to fructose in the presence of the above xylose isomerase.
    Type: Grant
    Filed: August 27, 1993
    Date of Patent: May 2, 1995
    Assignees: Nihon Shokuhin Kako Co., Ltd., Shiogezo Udaka
    Inventors: Shigezo Udaka, Kenji Sakaguchi, Hideo Yamagata, Koen Dekker
  • Patent number: 5384257
    Abstract: A method for selecting amino acid residues is disclosed which upon replacement will give rise to an enzyme with an altered pH optimum. The method is specific for metalloenzymes which are inactivated at low pH due to the dissociation of the metal ions. The method is based on altering the pK.sub.a of the metal coordinating ligands or altering the K.sub.ass for the metal binding. New glucose isomerases with an altered pH optimum are provided according to this method. These altered properties enable starch degradation to be performed at lower pH values.
    Type: Grant
    Filed: August 26, 1993
    Date of Patent: January 24, 1995
    Assignees: Gist-brocades, N.V., Plant Genetics System, NV
    Inventors: Anne-Marie Lambeir, Ignace Lasters, Nadir Mrabet, Wilhelmus J. Quax, Jan M. Van der Laan, Onno Misset
  • Patent number: 5376536
    Abstract: New mutant glucose isomerases are provided exhibiting improved properties under application conditions. These glucose isomerases are obtained by expression of a gene encoding said enzyme, having an amino acid sequence which differs at least in one amino acid from the wildtype glucose isomerase. Preferred mutant enzymes are those derived from Actinoplanes missouriensis glucose isomerase.
    Type: Grant
    Filed: January 10, 1991
    Date of Patent: December 27, 1994
    Assignees: Gist-Brocades, N.V., Plant Genetic Systems, N.V.
    Inventors: Wilhemus J. Quax, Rudolf G. M. Luiten, Paul W. Schuurhuizen, Nadir Mrabet
  • Patent number: 5340738
    Abstract: A method for selecting amino acid residues is disclosed which upon replacement will give rise to an enzyme with an altered pH optimum. The method is specific for metalloenzymes which are inactivated at low pH due to the dissociation of the metal ions. The method is based on altering the pK.sub.a of the metal coordinating ligands or altering the K.sub.ass for the metal binding. New glucose isomerases with an altered pH optimum are provided according to this method. These altered properties enable starch degradation to be performed at lower pH values.
    Type: Grant
    Filed: January 4, 1991
    Date of Patent: August 23, 1994
    Assignees: Gist-brocades, N.V., Plant Genetic Systems, N.V.
    Inventors: Anne-Marie Lambeir, Ignace Lasters, Nadir Mrabet, Wilhelmus J. Quax, Jan M. Van der Laan, Onno Misset
  • Patent number: 5310665
    Abstract: A method for selecting amino acid residues is disclosed which upon replacement will give rise to an enzyme with an altered substrate specificity. New mutant glucose isomerases with an altered substrate specificity are provided according to this method. These altered properties are useful in starch degradation and in other sugar conversion reactions.
    Type: Grant
    Filed: January 4, 1991
    Date of Patent: May 10, 1994
    Assignee: Gist-brocades, N.V.
    Inventors: Anne-Marie Lambeir, Ignace Lasters, Wilhemus J. Quax, Jan M. Van der Laan