Involving Dehydrogenase Patents (Class 435/26)
  • Patent number: 10711314
    Abstract: Methods of diagnosing subjects having a cell proliferation-related disorder or suspected of having a cell proliferation-related disorder associated with mutant IDH enzymes with 2HG neoactivity.
    Type: Grant
    Filed: April 26, 2018
    Date of Patent: July 14, 2020
    Assignee: Agios Pharmaceuticals, Inc.
    Inventors: Leonard Luan C. Dang, Stefan Gross, Hyun Gyung Jang, Shengfang Jin, Shin-San Michael Su, Craig Thompson
  • Patent number: 10613008
    Abstract: A sensing sensor includes a main body portion, a piezoelectric resonator, a connecting terminal, and an information storage. The main body portion includes a supply region to which the sample solution is supplied. The piezoelectric resonator is disposed to face the supply region and includes a capturing layer that captures a sensing object. The connecting terminal is configured to attachably/detachably connect a conductive path connected to an electrode of the piezoelectric resonator to a frequency measuring unit. The information storage stores calibration curve information to specify a calibration curve that indicates a relationship between a density of the sensing object and a frequency variation amount of the piezoelectric resonator before and after supplying the sample solution.
    Type: Grant
    Filed: January 3, 2018
    Date of Patent: April 7, 2020
    Assignee: NIHON DEMPA KOGYO CO., LTD.
    Inventors: Shunichi Wakamatsu, Wakako Shinobu
  • Patent number: 10533999
    Abstract: A process for stabilizing glutamate dehydrogenase (GDH) from a bacterium of the Clostridium genus, in an aqueous solution, in order to maintain the antigenic properties thereof, includes the step of mixing the glutamate dehydrogenase and a stabilizing composition which is a carboxylic acid having a carbon-based chain of at least 3 carbon atoms and comprising at least 2 —COOH groups, or a salt thereof. GDH compositions thus stabilized and a method of detecting the presence of bacteria of the Clostridium genus are also disclosed.
    Type: Grant
    Filed: December 8, 2014
    Date of Patent: January 14, 2020
    Assignee: BIOMERIEUX
    Inventors: Florence Bettsworth, Jérôme Martinez
  • Patent number: 10495598
    Abstract: An electrode that comprises a nanostructured material that comprises pyrolyzed date palm leaves that are obtained from a pyrolysis of an agro-waste containing date palm leaves in an inert gas and in a temperature range of 800 to 1600° C., an electrochemical cell thereof, and a method of determining a hydroquinone concentration in a hydroquinone-containing solution with the electrochemical cell. Various combinations of embodiments are also provided.
    Type: Grant
    Filed: June 14, 2019
    Date of Patent: December 3, 2019
    Assignee: King Fahd University of Petroleum and Minerals
    Inventor: Md. Abdul Aziz
  • Patent number: 10445580
    Abstract: Provided are an image extraction system, an image extraction method, and an image extraction program that extract an image matched with a theme. A first theme and a second theme are determined from a plurality of images. In a case in which the determined second theme is changed, the changed second theme is input. A first image extraction process that extracts an image from the plurality of images on the basis of the first theme is performed and a second image extraction process that extracts an image from the plurality of images on the basis of the second theme is performed. The extracted images are displayed.
    Type: Grant
    Filed: August 21, 2017
    Date of Patent: October 15, 2019
    Assignee: FUJIFILM Corporation
    Inventor: Tetsuya Matsumoto
  • Patent number: 10400262
    Abstract: A biosensor for the detection of an analyte such as ascorbic acid is provided. Also provided is a device comprising the biosensor. In addition, methods of detecting analytes such as ascorbic acid in a sample, and methods of a point-of-care diagnosis of eye disease and eye injury are provided.
    Type: Grant
    Filed: April 1, 2016
    Date of Patent: September 3, 2019
    Assignees: The Board of Trustees of the University of Illinois, Carle Health Care Incorporated
    Inventors: Dipanjan Pan, Santosh Kumar Misra, Manas Ranjan Gartia, Leanne T. Labriola
  • Patent number: 9982309
    Abstract: Methods of treating and evaluating subjects having neoactive mutants of IDH (e.g., IDH1 or IDH2).
    Type: Grant
    Filed: August 16, 2016
    Date of Patent: May 29, 2018
    Assignee: AGIOS PHARMACEUTICALS, INC.
    Inventors: Leonard Luan C. Dang, Stefan Gross, Hyun Gyung Jang, Shengfang Jin, Shin-San Michael Su, Craig Thompson
  • Patent number: 9791408
    Abstract: Proteins that are electrophoretically separated in a gel are derivatized to produce fluorescent emissions by incorporating halo-substituted organic compounds into one or both of the electrode buffer solutions at the two ends of the gel. The halo-substituted compounds used are ones that bear an electric charge at the pH of the buffer solutions and gel, and the polarity of the charge on the compounds is such that the compounds migrate from the electrode buffer into the gel under the electrophoretic influence concurrently with the migration of the proteins into the gel. Once the proteins are separated and distributed within the gel and the gel is fully penetrated with the halo-substituted compounds, the gel is irradiated with ultraviolet light to induce a reaction between the halo-substituted compounds and the proteins through the tryptophan residues on the proteins, producing fluorescent reaction products.
    Type: Grant
    Filed: March 9, 2015
    Date of Patent: October 17, 2017
    Assignee: Bio-Rad Laboratories, Inc.
    Inventor: Christopher Belisle
  • Patent number: 9739774
    Abstract: One example discloses a substance detection device, including: a substrate configured to carry a substance; wherein the substrate has a length and a substance loss along the length of the substrate; a test region coupled to the substrate and configured to bond to at least a portion of the substance; a sensor coupled to the substrate at a fixed location along the length and having a sensing signal output; an integration circuit coupled to the sensor and configured to integrate, over a time interval, a signal from the sensing signal output; and a detection circuit coupled to the integration circuit and configured to output a substance detected signal if the integrated sensing signal output signal deviates from the substance loss corresponding to the fixed sensor location.
    Type: Grant
    Filed: September 3, 2015
    Date of Patent: August 22, 2017
    Assignee: NXP B.V.
    Inventors: Axel Nackaerts, Viet Hoang Nguyen
  • Patent number: 9670523
    Abstract: The present invention discloses functional nitrite reductase as a potential drug target for anti-tubercular drug development. The present invention also relates to the development of an easy method for identification of nitrite in clinical samples as well as its correlation with the severity of the disease. Presence of active as well as dormant/latent stages of Mycobacterium tuberculosis (MTB) could be identified from nitrite in clinical samples like sputum of potential TB patients.
    Type: Grant
    Filed: November 3, 2014
    Date of Patent: June 6, 2017
    Assignee: Council of Scientific and Industrial Research
    Inventor: Dhiman Sarkar
  • Patent number: 9637774
    Abstract: Compositions, devices, kits and methods are disclosed for assaying glucose with a glycosylated, modified flavin adenine dinucleotide-dependent glucose dehydrogenase (FAD-GDH), variant thereof or an active fragment thereof, where at least one asparagine residue at positions N2, N168 and N346 of mature, wild-type A. oryzae FAD-GDH according to SEQ ID NO:2 is substituted by one or more amino acids not suitable for glycosylation, thereby eliminating or inactivating, respectively, a potential glycosylation site at this position.
    Type: Grant
    Filed: October 30, 2014
    Date of Patent: May 2, 2017
    Assignee: Roche Diabetes Care, Inc.
    Inventors: Hartmut Duefel, Thomas Meier, Michael Tacke
  • Patent number: 9568477
    Abstract: A system for identifying a bio-marker using mass spectroscopy is provided that includes a sample receptacle configured to receive a tissue sample, a mass spectrometry apparatus configured to receive the tissue sample and analyze the tissue sample using a mass spectrometry process to generate mass spectrometry data, and a computer system that includes a computer processor having access to a non-transitory, computer-readable storage medium having stored thereon instructions. The instructions cause the computer processor to: receive the mass spectrometry data from the mass spectrometry apparatus; analyze the mass spectrometry data to determine a presence of 2-Hydroxyglutarate (2-HG) in the tissue sample; and generate a report indicating a health of the tissue sample based on the presence of 2-HG in the tissue sample.
    Type: Grant
    Filed: December 30, 2013
    Date of Patent: February 14, 2017
    Assignee: Brigham and Women's Hospital, Inc.
    Inventor: Nathalie Agar
  • Patent number: 9453844
    Abstract: Methods and assays for detecting S-sulfhydration of amino acids in proteins, polypeptides and peptides are provided. The method is a two-step “tag-switch” method employing two reagents consecutively to specifically label, with a detectable label, persulfide (—S—SH) linkages in proteins, polypeptides and peptides.
    Type: Grant
    Filed: October 7, 2014
    Date of Patent: September 27, 2016
    Assignee: Washington State University
    Inventors: Ming Xian, Dehui Zhang
  • Patent number: 9266852
    Abstract: Disclosed is a daidzein derivative as represented by formula (I), or pharmaceutically acceptable salt thereof. Compared to the daidzein in the prior art, the daidzein derivative hydrochloride, in particular to the 7-O—N,N-diethyl-aminoacetyl daidzein hydrochloride, prepared by the daidzein derivative of the present invention, has better solubility and effect for treating cardiovascular diseases.
    Type: Grant
    Filed: November 28, 2012
    Date of Patent: February 23, 2016
    Assignee: Anhui Biochem Bio-Pharmaceutical Co., Ltd
    Inventors: Jun Bai, Caiyue Shen, Shigao Hu
  • Patent number: 9096825
    Abstract: The invention relates to 12 ?-hydroxysteroid dehydrogenases, nucleic acid sequences coding for the same, expression cassettes and vectors, recombinant microorganisms containing the corresponding coding nucleic acid sequences, a method for producing said 12 ?-hydroxysteroid dehydrogenases, a method for enzymatic oxidation of 12 ?-hydroxysteroids using said enzyme, a method for enzymatic reduction of 12-ketosteroids using said enzyme, a method for qualitative or quantitative determination of 12-ketosteroids and/or 12?-hydroxysteroids using said 12?-hydroxysteroid dehydrogenases and a method for production of ursodesoxycholic acid, comprising the enzyme-catalyzed cholic acid oxidation using said 12 ?-hydroxysteroid dehydrogenases.
    Type: Grant
    Filed: May 27, 2011
    Date of Patent: August 4, 2015
    Assignee: Pharmazell GmbH
    Inventors: Rolf Schmid, Michael Braun, Luo Liu, Arno Aigner, Dirk Weuster-Botz
  • Patent number: 9097659
    Abstract: The present disclosure relates to an electrochemical test sensor for detecting the concentration of an analyte in a fluid sample. The test sensor includes a working electrode, a counter electrode, and a trigger electrode. A temporary protective layer overlies the trigger electrode and helps to maintain the function of the trigger electrode during test sensor manufacture.
    Type: Grant
    Filed: March 14, 2013
    Date of Patent: August 4, 2015
    Assignee: Bayer HealthCare LLC
    Inventors: Andy Edelbrock, Steven C. Charlton
  • Patent number: 9045792
    Abstract: The present invention relates to a testing system for analyzing a risk of developing organ failure or presence of organ failure, said system comprising a disposable testing device arranged to receive a biological sample, which testing system is further arranged with means for measuring within said sample the total amount of at least a first biological marker in the form of an intracellular enzyme, and comprising means for analyzing the measured value of said intracellular enzyme, and based on the results thereof being arranged to communicate the risk of developing organ failure or presence of organ failure to an operator of said testing system. The present invention also relates to a method for analyzing a risk of developing organ failure, or analyzing presence of organ failure.
    Type: Grant
    Filed: September 14, 2011
    Date of Patent: June 2, 2015
    Assignee: Calmark Sweden AB
    Inventors: Mathias Karlsson, Sofia Hiort af Ornäs
  • Patent number: 9034887
    Abstract: A method of identifying an antifungal agent which targets a DHODH protein (alias PyrE, dihydroorotate dehydrogenase, EC: 1.3.99.11) of a fungus comprising contacting a candidate substance with a fungal DHODH protein and determining whether the candidate substance binds or modulates the DHODH protein, wherein binding or modulation indicates that the candidate substance is an antifungal agent. Specific examples concern Aspergillus fumigatus and Candida albicans DHODH proteins. DHODH inhibitors with a Quinazolinone core are also disclosed.
    Type: Grant
    Filed: May 1, 2009
    Date of Patent: May 19, 2015
    Assignee: F2G Limited
    Inventors: Jason David Oliver, John Leslie Thain, Michael John Bromley, Graham Edward Morris Sibley, Michael Birch
  • Publication number: 20150132769
    Abstract: Compositions and methods for screening for a disease or a disorder associated with a deficiency in frataxin in a subject using biomarkers for diseases or disorders associated with a deficiency in frataxin are disclosed. The compositions and methods include determining the acetylation status of mito-chondrial proteins. Also disclosed are methods of detecting progression of a disease or a disorder associated with a deficiency in frataxin in a subject and methods of monitoring effectiveness of a therapy for diseases or disorders associated with a deficiency in frataxin.
    Type: Application
    Filed: March 1, 2013
    Publication date: May 14, 2015
    Inventors: R. Mark Payne, Gregory R. Wagner, Clifford M. Babbey, P. Melanie Pride
  • Publication number: 20150127078
    Abstract: A method of determining hypoxia in fetal scalp blood sampled during labor including the determination of total lactate dehydrogenase (LDH) in plasma obtained from the sample. The method can include the additional determination of K, Mg, Ca, AST, ALT, lactate in the plasma and/or blood. Increased values of one or more of LDH, Mg, Ca, AST, ALT, lactate are indicative of hypoxia in the fetus. Also disclosed is the use of a plasma separation apparatus in the method.
    Type: Application
    Filed: January 8, 2015
    Publication date: May 7, 2015
    Inventors: Mathias Karlsson, Sofia Hiort af Ornäs
  • Publication number: 20150126580
    Abstract: Methods for diagnosing and treating cancer associated with an oncogenic Kras mutation in a subject are provided.
    Type: Application
    Filed: December 19, 2012
    Publication date: May 7, 2015
    Inventors: Ronald A. DePinho, Lewis Cantley, Alec C. Kimmelman, Haoqiang Ying, Costas A. Lyssiotis
  • Publication number: 20150118218
    Abstract: The disclosure relates to methods of detecting a neural injury biomarker in a biological sample. The method includes subjecting a biological sample to an assay disclosed that produces a measurable signal and detecting the measurable signal. The presence or absence of the measurable signal indicates the presence or absence of the neural injury biomarker in the sample, and thereby diagnosing a subject as having a neural injury. The disclosure further relates to methods of determining the state of a subject's neural injury. Further disclosed are systems and devices useful in carrying out the methods disclosed.
    Type: Application
    Filed: June 11, 2012
    Publication date: April 30, 2015
    Applicant: CORNELL UNIVERSITY
    Inventors: Alexander J.A. Travis, Roy Cohen
  • Patent number: 9012215
    Abstract: Using the methods of the present invention, intermediate (int) levels of aldehyde dehydrogenase (ALDH) activity reliably distinguished leukemic CD34+CD38? cells capable of engrafting immunodeficient mice, from residual normal hematopoietic stem cells that exhibited relatively higher ALDH activity. Minimal residual disease (MRD) detected during complete remission was enriched for the CD34+CD38?ALDHint leukemic cells, and the presence of these cells after therapy highly correlated with subsequent clinical relapse. The methods of the present invention can distinguish normal from leukemic CD34+CD38? cells, and identifies those AML cells associated with relapse. Methods of prediction of relapse of AML patients and methods of treatment are also provided.
    Type: Grant
    Filed: September 24, 2012
    Date of Patent: April 21, 2015
    Assignee: The Johns Hopkins University
    Inventors: Jonathan Michael Gerber, Richard John Jones
  • Publication number: 20150101929
    Abstract: The present invention relates to a stabilizing composition useful for improving the stability of reagent for redox reaction, and a reagent composition for redox reaction having an improved stability. The reagent composition for redox reaction can be applied for a reagent for electrochemical biosensor.
    Type: Application
    Filed: January 25, 2013
    Publication date: April 16, 2015
    Inventors: Sung-Kwon Jung, Moon Hwan Kim, Eun Hye Im, Myeongho Lee, Ung Ki Lee, Geun Sig Cha, Hakhyun Nam
  • Patent number: 8999662
    Abstract: Provided is a dry reagent that allows measuring with good precision, in accordance with a transmission method that utilizes light of the ultraviolet region, increases or decreases of a nicotinamide coenzyme, in order to quantify a component contained in a liquid sample. A dry reagent 4 for performing a quantitative analysis of a specific component in a liquid sample S contains a nicotinamide coenzyme and a leveling agent for smoothing the dry reagent 4. Increases or decreases of the nicotinamide coenzyme are measured in accordance with a transmission method that utilizes light of the ultraviolet region. The leveling agent is a combination of an alkali and at least one type selected from among a saccharide and a surfactant.
    Type: Grant
    Filed: January 17, 2013
    Date of Patent: April 7, 2015
    Assignee: ARKRAY, Inc.
    Inventors: Yui Yari, Toshihiro Imai, Tsutomu Nakamura
  • Patent number: 8999691
    Abstract: Disclosed is a modified glucose dehydrogenases that has dramatically increased productivity in Escherichia coli and dramatically increased thermal stability, which is obtained by introducing specific amino acid mutations to glucose dehydrogenase derived from Botryotinia fuckeliana. Also disclosed is a modified glucose dehydrogenases that has dramatically increased productivity in E. coli and dramatically increased thermal stability, which is obtained by replacing two amino acid residues in glucose dehydrogenase of fungal origin with cysteine residues. The novel glucose dehydrogenase has a low reactivity to xylose.
    Type: Grant
    Filed: June 29, 2011
    Date of Patent: April 7, 2015
    Assignee: Ultizyme International Ltd.
    Inventors: Koji Sode, Kazushige Mori
  • Patent number: 8999661
    Abstract: The invention provides compounds and methods of their use in the detection of apoptosis and necrosis both in vitro and in vivo. Also provided are compounds and methods of their use in selective delivery of agents to cells undergoing apoptosis or necrosis. The compounds and methods are based on conjugates formed with a dehydrogenase such as lactate dehydrogenase, alcohol dehydrogenase, aldehyde dehydrogenase, and malate dehydrogenase. The compounds and methods are useful in the diagnosis and treatment of conditions characterized by apoptosis, including cancer, cardiac disease, neurologic disease including stroke, and autoimmunity. The compounds and methods offer distinct advantages over corresponding compounds and methods based on Annexin V. Also provided are methods for screening for compounds that modulate, i.e., inhibit or promote, apoptosis.
    Type: Grant
    Filed: July 29, 2009
    Date of Patent: April 7, 2015
    Assignee: Dana-Farber Cancer Institute, Inc.
    Inventors: Andrew Kung, Pallab Banerjee
  • Publication number: 20150093771
    Abstract: An analytical device for analysis of chemical or biological samples, a method of using such a device, based on rotation of the device, integrated sample dosing and optical detection, and a system comprising such a device are disclosed. The analytical device comprises a device body having a liquid processing unit. The liquid processing unit comprises a mixing chamber for mixing a sample with a reagent, a sample dosing chamber for delivering a defined volume of the sample to the mixing chamber, and a reagent channel for delivering the reagent to be mixed with the sample, wherein the mixing chamber also serves as a detection chamber.
    Type: Application
    Filed: December 2, 2014
    Publication date: April 2, 2015
    Inventors: Patrick Griss, Rainer Jaeggi, Goran Savatic, Vuk Siljegovic
  • Publication number: 20150079584
    Abstract: A three dimensional cell culture and bioreactor system is provided. The system comprises one or more cell culture chamber. Each cell culture chamber comprises an inlet port and an outlet port in fluid communication with the cell culture chamber. The cell culture chambers may be segregated or in fluid communication with one another. The systems may be used to conduct drug efficacy test, isolate certain cell types from a complex tissue sample of multiple cell types, allow for the ex vivo culturing of patient tissue samples to help guide the course of treatment, and conduct co-culture experiments.
    Type: Application
    Filed: March 17, 2014
    Publication date: March 19, 2015
    Inventors: Matthew R. Gevaert, David E. Orr, Hal Crosswell
  • Publication number: 20150079617
    Abstract: The invention relates to a method for determining biochemical parameters of a body fluid, wherein a sample of said body fluid in the form of a droplet is transported through a channel of a microfluidic system using a carrier liquid, mixed with a reagent thus initiating a chemical reaction between the sample and the reagent, and the result of the chemical reaction is measured, preferably with a spectrophotometer, whereby the said biochemical parameters of the body fluid are determined, characterised in that the material used for fabrication of the microfluidic system and the said carrier liquid is pair of Teflon and Fluorinert HFE-7100.
    Type: Application
    Filed: September 12, 2012
    Publication date: March 19, 2015
    Inventors: Piotr Garstecki, Marcin Izydorzak, Magdalena Sulima, Adam Warchulski
  • Publication number: 20150065365
    Abstract: An apparatus is provided for sensing an analyte in a fluid.
    Type: Application
    Filed: May 12, 2014
    Publication date: March 5, 2015
    Applicant: Invoy Technologies, L.L.C
    Inventor: Lubna Ahmad
  • Publication number: 20150064200
    Abstract: The present invention relates to methods, compositions, and diagnostic tests for treating and diagnosing a proliferative disease that result in dysregulation of 6-phosphogluconate dehydrogenase. In particular, the methods and compositions include treatment of gefitinib/erlotinib resistant proliferative diseases such as lung cancer using a 6-phosphogluconate dehydrogenase antagonist.
    Type: Application
    Filed: April 4, 2013
    Publication date: March 5, 2015
    Applicant: Beth Israel Deaconess Medical Center, Inc.
    Inventors: Vikas P. Sukhatme, Barden Chunkong Chang
  • Patent number: 8969060
    Abstract: The purpose of the present invention is to provide an FAD-conjugated glucose dehydrogenase that is hard to be inhibited by the inhibitors such as 1,10-phenanthroline. The present invention relates to a modified glucose dehydrogenase (GLD), comprising an amino acid sequence of a wild-type FAD-conjugated glucose dehydrogenase (GLD) represented by SEQ ID NO: 1 having a substitution of at least one amino acid residue selected from the group consisting of amino acid residues at positions 298, 338, 340, 341, 343, 352, 354, 424, 426, 431 and 432, wherein the modified GLD has a reduced susceptibility to an inhibitor, as compared with the wild-type GLD, especially to said modified GLD, which has 40% or more of a relative activity when determined in a system wherein the inhibitor coexists at a final concentration of 1 mM based on an enzymatic activity when determined in a system wherein the inhibitor does not coexist.
    Type: Grant
    Filed: August 30, 2012
    Date of Patent: March 3, 2015
    Assignee: Ikeda Food Research Co., Ltd.
    Inventors: Michinari Honda, Tsuyoshi Kameda, Fuminao Kobayashi
  • Publication number: 20150044716
    Abstract: The present invention relates to a method for detecting (D)-2-hydroxyglutarate or (D)-2-hydroxyadipic acid in a sample, the method comprising the steps of: a) contacting a sample with a reagent mixture, wherein said reagent mixture comprises: (i) a solvent, (ii) a dye having an oxidized state and a reduced state, wherein the reduced state can be distinguished from the oxidized state and wherein the dye is initially present in the oxidized state, (iii) an electron transfer agent, (iv) a (D)-2-hydroxyglutarate dehydrogenase enzyme, and (v) a cofactor; and b) detecting (D)-2-hydroxyglutarate or (D)-2-hydroxyadipic acid by measuring the production of the reduced state of the dye. The invention further pertains to a method for diagnosing and/or monitoring a (D)-2-hydroxy-glutarate-associated disease in a subject. Encompassed by the invention is also a method for diagnosing a mutation in an isocitrate dehydrogenase (IDH) gene or in a (D)-2-hydroxyglutarate (D2HG) dehydrogenase enzyme gene in a subject.
    Type: Application
    Filed: March 1, 2013
    Publication date: February 12, 2015
    Applicants: DKFZ Deutsches Krebsforschungszentrum, Ruprecht-Karls-Universität Heidelberg
    Inventors: Jörg Balss, Stefan Pusch, Andreas Von Deimling, Wolfgang Buckel
  • Patent number: 8951720
    Abstract: Systematic comparisons of samples of adjacent clinically non malignant and tumor tissue from cancer of the gingivo buccal complex obtained during surgical resection of the tumor revealed significant differences in protein expression between the cancer tissue and the adjacent clinically non malignant tissue in each patient. This study has identified a set of new proteins which can differentiate between the epithelial tissue from cancer patients which may be useful for high-through put early detection, prognosis, and potential targets for therapy.
    Type: Grant
    Filed: February 7, 2008
    Date of Patent: February 10, 2015
    Assignee: Council of Scientific and Industrial Research
    Inventors: Surekha Mahesh Zingde, Rukmini Balkrishna Govekar, Sadhana Kannan, Nikhil Sureshkumar Gadewal, Ketayun Ardeshir Dinshaw, Anil Keith D'Cruz, Kumar Alok Pathak, Roshan Farokh Chinoy, Jai Prakash Agarwal, Ravi Sirdeshmukh, Curam Sreenivasacharlu Sundaram
  • Publication number: 20150037257
    Abstract: Human-derived fibroblast cells with copy number variation for alpha-synuclein, and methods of use thereof, are provided. For example, compositions and methods for high through-put screening of potential therapies for neurodegenerative disease such as Parkinson's disease are provided.
    Type: Application
    Filed: June 15, 2012
    Publication date: February 5, 2015
    Inventors: Sally Mak, Birgitt Schüle, J. William Langston
  • Patent number: 8945864
    Abstract: The object of the present invention is to provide a method of determining 1,5-anhydroglucitol, including using (a) a protein which consists of the amino acid sequence of SEQ ID NO: 2; (b) a protein which consists of an amino acid sequence having deletion, substitution and/or addition of one or more amino acid residues in the amino acid sequence of SEQ ID NO: 2 and which has sorbose dehydrogenase activity; or (c) a protein which consists of an amino acid sequence having a homology of at least 60% with the amino acid sequence of SEQ ID NO: 2 and which has sorbose dehydrogenase activity.
    Type: Grant
    Filed: June 22, 2007
    Date of Patent: February 3, 2015
    Assignees: Ikeda Food Research Co., Ltd., Nippon Kayaku Kabushiki Kaisha
    Inventors: Emi Ishimaru, Hirokazu Sanada, Hironori Omura, Hideki Yoshioka, Shuhei Tsukamoto, Minoru Masuda
  • Patent number: 8945824
    Abstract: A method of determining hypoxia in fetal scalp blood sampled during labor comprises the determination of total lactate dehydrogenase (LDH) in plasma obtained from the sample. The method can comprise the additional determination of K, Mg, Ca, AST, ALT, lactate in the plasma and/or blood. Increased values of one or more of LDH, Mg, Ca, AST, ALT, lactate are indicative of hypoxia in the fetus. Also disclosed is the use of a plasma separation apparatus in the method.
    Type: Grant
    Filed: April 11, 2008
    Date of Patent: February 3, 2015
    Assignee: Calmark Sweden Aktiebolag
    Inventors: Mathias Karlsson, Sofia Hiort af Ornäs
  • Patent number: 8945359
    Abstract: The invention provides a flavin-binding glucose dehydrogenase exhibiting reduced fluctuation of activity depending on temperature environment, and a method for measuring glucose concentration using the flavin-binding glucose dehydrogenase. The flavin-binding glucose dehydrogenase has the following properties (1) to (3): (1) activity: which exhibits glucose dehydrogenase activity in the presence of an electron acceptor; (2) substrate specificity: which exhibits an activity of 10% or less against maltose, D-galactose, D-fructose, sorbitol, lactose and sucrose when the activity against D-glucose is defined as 100%; and (3) temperature characteristics: which exhibits lower fluctuation of activity in a wide temperature range of 10 to 50° C.
    Type: Grant
    Filed: February 26, 2013
    Date of Patent: February 3, 2015
    Assignee: Ikeda Food Research Co., Ltd.
    Inventors: Michinari Honda, Sayaka Taki, Ryo Takenaka
  • Publication number: 20150030544
    Abstract: Disclosed herein are compositions including a nanosensor that is sensitive to an analyte such that the nanosensor emits a fluorescent signal upon detecting the analyte, and a catalytic agent that catalyzes a reaction in which a target substrate is converted into one or more products, such that at least one of the one or more products is the analyte. In addition, methods of using the nanosensor-catalytic agent compositions to detect a target substrate are disclosed.
    Type: Application
    Filed: March 6, 2013
    Publication date: January 29, 2015
    Inventors: Heather A. Clark, Kevin Joseph Cash
  • Publication number: 20150025339
    Abstract: Methods of classifying pleural fluid are disclosed. The methods typically include determining the level of indicator nanoparticles, such as lipids, particularly large lipids, in the pleural fluid of a subject. The level of lipids can be determined by nuclear magnetic resonance (NMR), such as proton NMR (1H-NMR) by measuring the NMR signal corresponding to methyl protons, methylene protons, methene protons, or combinations thereof. The level of large lipids in pleural fluid can be carried out in vitro on a sample of pleural fluid obtained from the subject or in vivo using magnetic resonance spectroscopy (MRS). The pleural fluid can be classified as exudate or transudate with a sensitivity, selectivity, or combination thereof of 85%, 90%, 95%, 99%, or higher, a selectivity of 85%, 90%, 95%, 99%. The method can be coupled with diagnosing and/or treating the subject with a disease, disorder, or condition.
    Type: Application
    Filed: July 18, 2014
    Publication date: January 22, 2015
    Inventor: Ching-Wan Lam
  • Publication number: 20150010936
    Abstract: The present invention provides a means and method useful for measurement of a total branched-chain amino acid concentration. Specifically, the present invention provides a modified enzyme in which at least one amino acid residue is mutated so as to improve a property of a leucine dehydrogenase which is associated with the measurement of the total branched-chain amino acids, such as, for example, substrate specificities of leucine dehydrogenase for total branched-chain amino acids, activity of leucine dehydrogenase for any branched-chain amino acids, and thermal stability of leucine dehydrogenase; and a method of analyzing the total branched-chain amino acids, comprising measuring the total branched-chain amino acids contained in a test sample using the modified enzyme.
    Type: Application
    Filed: September 19, 2014
    Publication date: January 8, 2015
    Applicant: AJINOMOTO CO., INC.
    Inventors: Wataru Hoshino, Yuya Kodama, Toshimi Mizukoshi, Uno Tagami
  • Publication number: 20150005193
    Abstract: Disclosed herein are “equipment-free” flow-through assay devices based on patterned porous media, methods of making same, and methods of using same. The porous, hydrophilic media are patterned with hydrophobic barriers for performing assays on liquids.
    Type: Application
    Filed: June 20, 2014
    Publication date: January 1, 2015
    Inventors: Scott Thomas Phillips, Gregory Gerald Lewis, Jessica Sloane Robbins
  • Patent number: 8921061
    Abstract: Reagent materials and associated test elements are provided. In one embodiment, a test element having dual functionality includes a first coenzyme-dependent enzyme or a substrate for the first enzyme, a second coenzyme-dependent enzyme or a substrate for the second enzyme, and a coenzyme selected from the group consisting of thio-NAD, thio-NADP, and a compound according to formula (I). In one aspect, the first analyte is hydroxybutyrate and the first enzyme is a hydroxybutyrate dehydrogenase, and the second analyte is glucose and the second enzyme is a glucose dehydrogenase or a glucose oxidase. Other aspects of the subject application are directed to unique reagent materials. Further embodiments, forms, objects, features, advantages, aspects, and benefits shall become apparent from the description and drawings.
    Type: Grant
    Filed: November 2, 2012
    Date of Patent: December 30, 2014
    Assignee: Roche Diagnostics Operations, Inc.
    Inventor: Christopher D. Wilsey
  • Patent number: 8921065
    Abstract: A reagent composition containing GDH-PQQ as an enzyme-co-factor and screen-printed on working and counter electrodes of electrochemical biosensors, maintains activity of the enzyme reagents by proper selection of components. A preferred composition includes hydrophilic polymers, amorphous untreated silica, buffers, surfactants, and a mediator. For example, the biosensor is useful in the amperometric determination of glucose.
    Type: Grant
    Filed: March 3, 2006
    Date of Patent: December 30, 2014
    Assignee: Bayer HealthCare LLC
    Inventors: Amy H. Chu, Hope G. Spradlin
  • Publication number: 20140377756
    Abstract: A method for quantifying skin changes caused by six external evils and a method of screening skin condition-improving materials using the quantification method are described. More specifically, disclosed are a method of measuring cellular changes caused by external stimuli in a skin cell culture system, in which the degree of cellular changes obtained by applying suitable stimuli of six external evils to skin cells being cultured is measured by cellular biochemical methods, such that the conceptual effects of six external evils suggested in the prior art can be scientifically and quantitatively expressed, and a method of screening skin condition-improving materials using the measurement method.
    Type: Application
    Filed: September 5, 2014
    Publication date: December 25, 2014
    Inventors: Jin Young LEE, Sun Sang KWON, Myeong Hoon YEOM, Duck Hee KIM, Han Kon KIM
  • Publication number: 20140370520
    Abstract: The invention relates to a method for diagnosing cancer, particularly bladder or prostate cancer using compounds of general formula (I): wherein R1, R2, R3, R4, R5 X, Y and z are as defined herein.
    Type: Application
    Filed: December 8, 2011
    Publication date: December 18, 2014
    Applicant: University College Cardiff Constultants Limited
    Inventors: Ian Weeks, Mohammad Jaffar, Richard Knox
  • Publication number: 20140363835
    Abstract: Dry reagent compositions are disclosed for maintaining and preserving enzymatic activity. Also disclosed are test elements incorporating the same and methods of making and using the same for determining the presence or amount of an analyte in a body fluid sample. The dry reagent compositions can include components such as a dehydrogenase, a redox cofactor, an agent capable of eliciting at least one optical change in an optical property of an indicator reagent in the presence of redox equivalents, an indicator reagent, and at least one compatible solute being ectoine or a derivative thereof.
    Type: Application
    Filed: August 22, 2014
    Publication date: December 11, 2014
    Inventors: Gabriele Chemnitius, Otto Gaa, Thomas Nagel, Karl Recht
  • Publication number: 20140349312
    Abstract: The present invention relates to a culture support for cultivating hematopoietic stem cells (HSCs) and/or hematopoietic progenitors (HPs), comprising a calcium biomaterial, osteoclasts, endothelial cells and mesenchymatous stem cells (MSCs) and/or osteoblasts and/or adipocytes. The present invention also relates to a method for preparing such a culture support, and an in vitro HSC and/or HP cultivation method. The use of such a culture support for studying cellular mechanisms involved in hematopoiesis and/or differentiation of HSC/HPs and/or for studying the efficacy and/or the toxicity of a medicament candidate is also described.
    Type: Application
    Filed: August 14, 2012
    Publication date: November 27, 2014
    Applicants: Institut Nationalde la Sante et de la Recherche Medicale (INSERM), ETAT FRANCAIS (MINISTERE DE LA DEFENSE), SERVICE DE SANTE DES ARMEES
    Inventors: Jean-Jacques Lataillade, Marie-Caroline Le Bousse-Kerdiles
  • Publication number: 20140335069
    Abstract: Aspects of embodiments may include methods for automated enzymatic detection of glucose-6-phosphate dehydrogenase (G6PD) activity. Aspects of embodiments may include methods for enzymatic detection of G6PD activity in droplets in oil. Aspects of embodiments may include a system including a droplet actuator. Aspects of embodiments may include a treatment method.
    Type: Application
    Filed: November 20, 2012
    Publication date: November 13, 2014
    Applicant: ADVANCED LIQUID LOGIC, INC.
    Inventors: Carrie A. Graham, Allen E. Eckhardt