Rhizobium Or Agrobacterium Patents (Class 435/252.2)
  • Patent number: 11091766
    Abstract: Production of transformed plants is achieved by placing Agrobacterium-inoculated explants directly onto a medium containing selective agents for suppressing growth of Agrobacterium and killing non-transformed explant cells.
    Type: Grant
    Filed: May 11, 2018
    Date of Patent: August 17, 2021
    Assignee: Monsanto Technology LLC
    Inventors: Anisha Akula, David R. Duncan, Brenda A. Lowe, Michael T. Mann, William L. Petersen, Jyoti R. Rout, David D. Songstad, Joel B. Wilks, Wanggen Zhang
  • Patent number: 10934553
    Abstract: A process of transiently transfecting a plant or leaves on a plant, comprising contacting said plant or said leaves with a suspension comprising Agrobacterium cells of strain CryX or a derivative strain of strain CryX, wherein said derivative strain has the chromosomal background of strain CryX or said derivative strain contains the vir plasmid of strain CryX or a derivative of said vir plasmid.
    Type: Grant
    Filed: July 23, 2019
    Date of Patent: March 2, 2021
    Assignee: Nomad Bioscience GmbH
    Inventors: Patrick Roemer, Luisa Bortesi, Doreen Tiede, Anatoli Giritch, Yuri Gleba
  • Patent number: 10676406
    Abstract: Hopanoids, hopanoids-producing nitrogen-fixing bacteria, and related formulations, systems and methods are described herein. In particular, hopanoids alone or in combination with hopanoid-producing nitrogen-fixing bacteria can be used as biofertilizer to stimulate plant growth and yield with enhanced tolerance to diverse stresses found in plant-microbe symbiotic microenvironments.
    Type: Grant
    Filed: October 9, 2018
    Date of Patent: June 9, 2020
    Assignee: CALIFORNIA INSTITUTE OF TECHNOLOGY
    Inventors: Dianne K. Newman, Gargi Kulkarni, Brittany Jo Belin, Eric Giraud, Antonio Molinaro, Alba Silipo
  • Patent number: 10640782
    Abstract: An object of the present invention is to identify a novel gene that effectively increases plant biomass and to provide said gene as well as techniques utilizing the same. This invention provides a nucleic acid encoding a protein, wherein the protein comprises the amino acid sequence of SEQ ID NO:1 and an amino acid sequence having at least 25% identity or at least 75% similarity to the amino acid sequence of SEQ ID NO:7 or 11, and has an activity to increase plant biomass, as well as techniques utilizing the same.
    Type: Grant
    Filed: October 9, 2015
    Date of Patent: May 5, 2020
    Assignees: JAPAN TOBACCO INC., RIKEN
    Inventors: Takeshi Nakano, Tadao Asami, Tomoko Miyaji, Ayumi Yamagami, Noriko Ishikawa
  • Patent number: 10631516
    Abstract: The present invention relates to new methods of selecting and breeding organisms, in particular organisms which exhibit symbiotic behaviour with symbionts, and to new organisms and symbiota developed thereby, such as plant or grass and endophyte symbiota. Multiple symbionts are deployed in multiple organisms and selected for improved symbiotic compatibility and performance early in the breeding process. Methods include producing improved organisms from germplasm, by inoculating host organism germplasm libraries with symbionts selected from symbiont libraries and selecting improved host organisms exhibiting desired symbiota characteristics, and selection of organism-symbiont associations with a desired genetic and metabolic profile by metagenomic analysis of nucleic acid libraries from an organism or organism-symbiont association.
    Type: Grant
    Filed: May 29, 2013
    Date of Patent: April 28, 2020
    Assignee: Agriculture Victoria Services PTY LTD
    Inventors: German Carlos Spangenberg, John White Forster, Noel Cogan, Yidong Ran, John Gregory Mason, Timothy Ivor Sawbridge, Kathryn Michaela Guthridge, Simone Jane Rochfort, Ben Cocks
  • Patent number: 10462990
    Abstract: The present invention relates to methods for enhancing at least one growth parameter of a leguminous plant via co-inoculation of a leguminous plant with at least one rhizobial microorganism together with at least one actinobacterial microorganism. In further aspects, the present invention also relates to leguminous plants co-inoculated with at least one rhizobial microorganism together with at least one actinobacterial microorganism, as well as specific actinobacterial strains and inoculant compositions which are useful in accordance with the present invention.
    Type: Grant
    Filed: June 19, 2015
    Date of Patent: November 5, 2019
    Assignee: The Flinders University of South Australia
    Inventors: Christopher Milton Mathew Franco, Hoang Xuyen Le, Ross Alexander Ballard
  • Patent number: 10435698
    Abstract: Methods of, and compositions for, assembling one or more transcription units in a genome without a linked selectable marker or other unwanted transcription unit are provided. Also provided methods of, and compositions for, assembling one or more transcription units in a genome with a reduced frequency of vector backbone.
    Type: Grant
    Filed: June 7, 2017
    Date of Patent: October 8, 2019
    Assignee: Monosanto Technology LLC
    Inventors: Larry A. Gilbertson, Susan J. Johnson
  • Patent number: 10287602
    Abstract: A process of expressing a sequence of interest in a plant, plant part, or plant cell culture, comprising: (a) providing a plant, plant part, or plant cell culture containing in cell nuclei a heterologous DNA having a sequence encoding an RNA replicon operably linked or linkable to a transcription promoter, wherein said sequence encoding an RNA replicon contains (i) sequences for replicon function of said RNA replicon, said sequences being derived from a sequence of a plant RNA virus, (ii) a sequence of interest, whereby said sequences for replicon function exhibit at selected localities of said sequences of said plant RNA virus function-conservative differences from said sequence of said plant RNA virus, said differences causing an increased frequency of replicon formation compared to an RNA replicon not exhibiting said differences; and (b) causing expression of said sequence of interest.
    Type: Grant
    Filed: January 5, 2016
    Date of Patent: May 14, 2019
    Assignee: Icon Genetics GmbH
    Inventors: Sylvestre Marillonnet, Carola Engler, Victor Klimyuk, Yuri Gleba
  • Patent number: 10240163
    Abstract: Provided are plants that express, or overexpress, a pPLAIII? protein. Constitutive or seed-specific expression of pPLAIII? protein in Arabidopsis increases seed oil content, the amount of C20 and C22 fatty acids, and the amount of C56, C58, and C60 triacylglycerols, effectively resulting in significantly higher oil yield per plant. Use of pPLAIII? is therefore an effective biotechnological tool to significantly increase plant yield, including oil, and the amount of high value long chain fatty acids in agricultural and horticultural crops, especially oilseed crops.
    Type: Grant
    Filed: September 25, 2015
    Date of Patent: March 26, 2019
    Assignees: Donald Danforth Plant Science Center, The Curators of the University of Missouri
    Inventors: Xuemin Wang, Maoyin Li
  • Patent number: 10131585
    Abstract: Hopanoids, hopanoids-producing nitrogen-fixing bacteria, and related formulations, systems and methods are described herein. In particular, hopanoids alone or in combination with hopanoid-producing nitrogen-fixing bacteria can be used as biofertilizer to stimulate plant growth and yield with enhanced tolerance to diverse stresses found in plant-microbe symbiotic microenvironments.
    Type: Grant
    Filed: October 19, 2016
    Date of Patent: November 20, 2018
    Assignee: CALIFORNIA INSTITUTE OF TECHNOLOGY
    Inventors: Dianne K. Newman, Gargi Kulkarni, Brittany Jo Belin, Eric Geraud, Antonio Molinaro, Alba Silipo
  • Patent number: 10072271
    Abstract: Compositions and methods for increasing plant growth and yield are provided. Compositions comprise the high yield gene TEL, promoters and enhancers to increase the expression of a TEL gene in a plant of interest. By enhancing the expression of at least one TEL gene in a plant, an improvement in plant growth and yield is achieved, resulting in an increase in crop yield in a field planted with such plants. A plant of interest may be transformed with a DNA construct comprising a promoter that is capable of driving expression in the plant operably linked to a coding sequence for a TEL gene. The DNA construct may comprise at least on enhancer that acts to increase expression of the TEL coding sequence. A promoter or enhancer can be inserted into the genome of the plant of interest at a site that increases the expression of the endogenous TEL coding sequence in the plant.
    Type: Grant
    Filed: December 20, 2012
    Date of Patent: September 11, 2018
    Assignee: HANGZHOU RUIFENG BIOTECHNOLOGY LIMITED INC.
    Inventors: Zhicheng Shen, Xianwen Zhang, Dongfang Wang, Jianhua Gao
  • Patent number: 10006036
    Abstract: A method of producing a stably transformed corn plant in a single container is demonstrated. This method allows for the automation of the transformation process and reduces labor, material, and ergonomic costs associated with traditional plant tissue culture systems.
    Type: Grant
    Filed: January 16, 2015
    Date of Patent: June 26, 2018
    Assignee: Monsanto Technology LLC
    Inventors: Anisha Akula, David R. Duncan, Brenda A. Lowe, Michael T. Mann, William L. Petersen, Jyoti R. Rout, David D. Songstad, Joel B. Wilks, Wanggen Zhang
  • Patent number: 9854808
    Abstract: The present invention relates to the use of a composition comprising a superoxide dismutase (SOD) enzyme and/or an active plant extract containing SOD, for stimulating the overexpression of plant antioxidant enzymatic proteins, stimulating plant vitality and/or increasing plant productivity and/or preventively or curatively protecting said plants against biotic or abiotic stresses.
    Type: Grant
    Filed: December 17, 2013
    Date of Patent: January 2, 2018
    Assignee: Bionov
    Inventors: Dominique Lacan, Alain Dreyer, Christian Yard, Benoit Lemaire
  • Patent number: 9790524
    Abstract: The present invention is to provide a preparation of variant recombinant whole cell biocatalysts, referred herein as “designer cells” having significantly enhanced carbonyl reductase activity for use in the efficient production of variant industrially important enantiomerically enriched alcohols. More specifically, the alcohol is optically pure ethyl (S)-4-chloro-3-hydroxybutyrate, which is useful as chiral building block and an intermediate for the production of hydroxymethylglutaryl-CoA (HMG-CoA) reductase inhibitors.
    Type: Grant
    Filed: April 17, 2014
    Date of Patent: October 17, 2017
    Assignee: COUNCIL OF SCIENTIFIC & INDUSTRIAL RESEARCH
    Inventors: Gautam Srivastava, Suneet Kaur, Ravinder Singh Jolly
  • Publication number: 20150143587
    Abstract: A method of producing a stably transformed corn plant in a single container is demonstrated. This method allows for the automation of the transformation process and reduces labor, material, and ergonomic costs associated with traditional plant tissue culture systems.
    Type: Application
    Filed: January 16, 2015
    Publication date: May 21, 2015
    Inventors: Anisha Akula, David R. Duncan, Brenda A. Lowe, Michael T. Mann, William L. Petersen, Jyoti R. Rout, David D. Songstad, Joel B. Wilks, Wanggen Zhang
  • Publication number: 20150143574
    Abstract: The invention relates to a new gene that is able to provide plants with resistance against pathogens, more preferably Verticillium, Ralstonia or Fusarium. Said gene is typical for Brassicaceae and encodes for a proteins having a sequence as depicted in FIG. 7 or FIG. 2A. Also provided are methods for enhancing the pathogen resistance of plants, wherein said plants preferably are Brassicaceae, but wherein the resistance also is functional in other plants. Further provided are host cells with a nucleotide construct encoding said protein.
    Type: Application
    Filed: May 24, 2013
    Publication date: May 21, 2015
    Inventors: Bart Pierre Hélène Joseph Thomma, Koste Abdissa Yadeta
  • Publication number: 20150135365
    Abstract: A method for enhancing various economically important yield-related traits in plants is provided. More specifically, a method for enhancing one or more yield-related traits in plants is provided, by modulating expression in a plant of a nucleic acid encoding a POI (protein of interest) polypeptide. Also provided are plants having modulated expression of a nucleic acid encoding a POI polypeptide, the plants having one or more enhanced yield-related traits compared with control plants Unknown POI-encoding nucleic acids and constructs comprising the same that useful in performing the methods of the invention are further provided.
    Type: Application
    Filed: March 15, 2013
    Publication date: May 14, 2015
    Inventors: Ana Isabel Sanz Molinero, Valerie Frankard
  • Publication number: 20150128305
    Abstract: The present invention provides herein a polynucleotide sequence encoding the tubby-like protein, CaTLP1, from chickpea (Cicer arientium L.) that is responsive to abiotic stress and is involved in plant growth and development. Further, the recombinant DNA construct and recombinant vector comprising the polynucleotide sequence encoding CaTLP1, host cell comprising the recombinant vector and a process for producing a transgenic plant that expresses the CaTLP1 protein are also provided herein.
    Type: Application
    Filed: May 7, 2013
    Publication date: May 7, 2015
    Inventors: Niranjan Chakraborty, Subhra Chakraborty, Asis Datta, Vijay Wardhan, Kishwer Jahan
  • Publication number: 20150111233
    Abstract: Luciferases which are different from those known heretofore have been desired. A luciferase mutant comprising an amino acid sequence in which at least one amino acid selected from the group consisting of valine at the position of 44, alanine at the position of 54 and tyrosine at the position of 138 is substituted with other amino acid(s) in the amino acid sequence of SEQ ID NO: 2.
    Type: Application
    Filed: October 17, 2014
    Publication date: April 23, 2015
    Inventors: Satoshi INOUYE, Junichi SATO
  • Publication number: 20150113682
    Abstract: The present invention discloses gene targets, constructs and methods for the genetic control of plant disease caused by nematodes of the genus Meloidogyne (root knot nematodes). The present invention relates to achieving a plant protective effect through the identification of target coding sequences and the use of recombinant DNA technologies for post-transcriptionally repressing or inhibiting expression of the target coding sequences in the cells of plant-parasitic nematodes. The disclosed gene targets show significant conservation at the nucleotide level between orthologs from different Meloidogyne species, facilitating genus-wide targeting by RNA interference.
    Type: Application
    Filed: October 1, 2014
    Publication date: April 23, 2015
    Inventors: JOHN D. BRADLEY, BRANDI J. CHIAPELLI, BINGLI GAO, JAMES P. MCCARTER, MICHELLE L. GASPER, DERYCK JEREMY WILLIAMS
  • Publication number: 20150105255
    Abstract: Derivatives of Agrobacterium vitis strain F2/5 are disclosed. These derivatives were generated following homologous recombination with an internal fragment of targeted genes resulting in gene disruption by insertion of a copy of suicide vector pVIK165. The genes disrupted were F-avi5813 encoding a phosphopantetheinyltransferase, F-avi4329 encoding an aminotransferase and F-avi0838 (rirA) encoding an iron responsive transcriptional regulator. Such derivatives control crown gall on grapevines. In addition, these derivatives did not induce roots necrosis but enhanced root development and callus formation. On young stem explants, it was shown as well that the F2/5 derivatives are necrosis-negative.
    Type: Application
    Filed: March 15, 2013
    Publication date: April 16, 2015
    Applicant: Cornell University
    Inventors: Thomas J. Burr, Desen Zheng
  • Publication number: 20150087028
    Abstract: The invention relates to recombinant expression of variant forms of M. thermophila CBH1a and homologs thereof, having improved thermoactivity, specific activity, and other desirable properties. Also provided are methods for producing ethanol and other valuable organic compounds by combining cellobiohydrolase variants with cellulosic materials.
    Type: Application
    Filed: March 12, 2013
    Publication date: March 26, 2015
    Inventors: Behnaz Behrouzian, Xinkai Xie, Kui Chan, Xiyun Zhang, Vesna Mitchell, Douglas A. Hattendorf
  • Publication number: 20150082490
    Abstract: The present invention relates to the field of RNA-mediated gene silencing in insect species. The present invention is based, in part, on the inventors' sequencing of genes from eucalyptus invasive species Gb pest, Glycaspis brimblecombei. In certain aspects, the invention provides Gb nucleic acids, derivatives thereof and the use of such nucleic acids and derivatives as Gb control agents.
    Type: Application
    Filed: April 22, 2013
    Publication date: March 19, 2015
    Inventors: Dror Avisar, Daniel Siegel, Ziv Shani
  • Publication number: 20150082482
    Abstract: The present invention relates to the field of RNA-mediated gene silencing in insect species. The present invention is based, in part, on the inventors' sequencing of genes from eucalyptus invasive species Bronze bug pest, Thaumastocoris peregrinus. In certain aspects, the invention provides Bronze bug nucleic acids, derivatives thereof and the use of such nucleic acids and derivatives as Bronze bug control agents.
    Type: Application
    Filed: April 19, 2013
    Publication date: March 19, 2015
    Applicant: Futuragene Israel Ltd.
    Inventors: Dror Avisar, Daniel Siegel, Ziv Shani
  • Publication number: 20150064751
    Abstract: The embodiments described herein pertain to cells, and methods for preparing cells, that can be used as biocatalysts by altering enzymes that compete for a substrate or product of a pathway of interest such that the targeted enzyme is sensitive to a site-specific protease, which protease is expressed but relocated in the cell to a site where it is not in contact with the targeted enzyme in the intact cell. Upon cell lysis, the protease contacts the target enzyme, which is then inactivated by protease cleavage.
    Type: Application
    Filed: November 14, 2014
    Publication date: March 5, 2015
    Applicants: The Board of Trustees of the Leland Stanford Junior University, GreenLight Biosciences, Inc.
    Inventor: James R. Swartz
  • Patent number: 8962918
    Abstract: The present invention provides isolated polynucleotide sequences encoding ?-mannosidase. The present invention further provides DNA constructs comprising the polynucleotide sequence coding for ?-mannosidase in sense or anti-sense orientation, RNAi constructs, recombinant vectors comprising the constructs, and host cells comprising the recombinant vector. The present invention further provides transgenic plants, plant cells, transgenic progeny and seeds expressing the polynucleotide with reduced ?-mannosidase protein accumulation, having enhanced fruit shelf life.
    Type: Grant
    Filed: July 9, 2009
    Date of Patent: February 24, 2015
    Assignee: National Institute of Plant Genome Research
    Inventors: Asis Datta, Subhra Chakraborty, Niranjan Chakraborty, Sumit Ghosh, Vijaykumar Siddesh Meli
  • Publication number: 20150052638
    Abstract: A process of transiently transfecting a plant or leaves on a plant, comprising contacting said plant or said leaves with a suspension comprising Agrobacterium cells of strain CryX or a derivative strain of strain CryX, wherein said derivative strain has the chromosomal background of strain CryX or said derivative strain contains the vir plasmid of strain CryX or a derivative of said vir plasmid.
    Type: Application
    Filed: April 3, 2013
    Publication date: February 19, 2015
    Inventors: Patrick Roemer, Luisa Bortesi, Doreen Tiede, Anatoli Giritch, Yuri Gleba
  • Publication number: 20150050708
    Abstract: Provided herein are non-naturally occurring microbial organisms having a formaldehyde fixation pathway and a formate assimilation pathway, which can further include a methanol metabolic pathway, a methanol oxidation pathway, a hydrogenase and/or a carbon monoxide dehydrogenase. These microbial organisms can further include a butadiene, 1,3-butanediol, crotyl alcohol or 3-buten-2-ol pathway. Additionally provided are methods of using such microbial organisms to produce butadiene, 1,3-butanediol, crotyl alcohol or 3-buten-2-ol.
    Type: Application
    Filed: March 14, 2014
    Publication date: February 19, 2015
    Applicant: Genomatica, Inc.
    Inventors: Anthony P. BURGARD, Robin E. OSTERHOUT, Priti PHARKYA, Stefan ANDRAE
  • Publication number: 20150044747
    Abstract: The invention relates, in part, to nucleic acid constructs, genetically modified host cells and methods employing such constructs and host cells to increase the production of 3-methyl-2-butenol from IPP. Thus, in some aspects, the invention provides a genetically modified host cell transformed with a nucleic acid construct encoding a fusion protein comprising a phosphatase capable of catalyzing the dephosphorylation of dimethylallyl diphosphate (DMAPP) linked to an IPP isomerase capable of converting IPP to DMAPP, wherein the nucleic acid construct is operably linked to a promoter. In some embodiments, the genetically modified host cell 5 further comprises a nucleic acid encoding a reductase that is capable of converting 3-methyl-2-butenol to 3-methyl-butanol. In some embodiments, the reductase is encoded by a nucleic acid construct introduced into the cell. In some embodiments, the IPP isomerase is a Type I isomerase. In some embodiments, the IPP isomerase is a Type II isomerase.
    Type: Application
    Filed: September 13, 2012
    Publication date: February 12, 2015
    Applicant: The Regents of the University of California
    Inventors: Howard Chou, Jay D. Keasling
  • Publication number: 20150033409
    Abstract: The present invention relates to expression cassettes comprising at least one transcription regulating nucleotide sequence obtainable from the group of genes of monocotyledonous plants consisting of caffeoyl-CoA-O-methyltransferase genes, C8,7-sterol isomerase genes, hydroxyproline-rich glycoprotein (HRGP) genes, lactate dehydrogenase genes, and chloroplast protein 12 like genes. More preferably the transcription regulating sequences are obtainable from Zea mays or Oryza sativa. The transcription regulating sequences are especially useful for root/kernel-preferential, leaf/endosperm-preferential, root/silk/kernel-preferential, or constitutive expression.
    Type: Application
    Filed: October 3, 2014
    Publication date: January 29, 2015
    Inventors: Hee-Sook Song, Marc Morra, Christian Dammann, Christina E. Roche, Effie Toren, Alleson Dobson
  • Publication number: 20150013031
    Abstract: The present invention relates to methods and compositions for improving the efficiency of Agrobacterium- and Rhizobium-mediated plant cell transformation by use of additional transformation enhancer sequences, such as overdrive or TSS sequences, operably linked to a T-DNA border sequence on a recombinant construct that comprises T-DNA.
    Type: Application
    Filed: June 3, 2014
    Publication date: January 8, 2015
    Inventors: Larry A. Gilbertson, Xudong Ye
  • Publication number: 20140377844
    Abstract: A method of deactivating an explosive composition being used in a blasting operation, which method comprises exposing the explosive composition to a micro-organism that is indigenous to the environment in which the explosive composition is being used and that is capable of producing an enzyme that degrades the explosive composition, wherein the explosive composition has associated with it a chemical inducing agent that promotes production of the enzyme by the micro-organism.
    Type: Application
    Filed: January 29, 2013
    Publication date: December 25, 2014
    Inventors: Clint Brearley, Steven Kotsonis, Kaiyan Liu, Thomas Smylie, Richard Goodridge
  • Publication number: 20140377860
    Abstract: The present invention concerns antibodies to Bv8 and the uses of same.
    Type: Application
    Filed: May 29, 2014
    Publication date: December 25, 2014
    Inventors: Xiumin Wu, Yan Wu, Lanlan Yu, Napoleone Ferrara, Wei-Ching Liang, Yu-Ju G. Meng, Janet Tien
  • Publication number: 20140371417
    Abstract: The invention provides non-naturally occurring microbial organisms having a 4-hydroxybutyrate, gamma-butyrolactone, 1,4-butanediol, 4-hydroxybutanal, 4-hydroxybutyryl-CoA and/or putrescine pathway and being capable of producing 4-hydroxybutyrate, wherein the microbial organism comprises one or more genetic modifications. The invention additionally provides methods of producing 4-hydroxybutyrate, gamma-butyrolactone, 1,4-butanediol, 4-hydroxybutanal, 4-hydroxybutyryl-CoA and/or putrescine or related products using the microbial organisms.
    Type: Application
    Filed: April 25, 2014
    Publication date: December 18, 2014
    Applicant: Genomatica, Inc.
    Inventors: Priti PHARKYA, Anthony P. BURGARD, Stephen J. VAN DIEN, Robin E. OSTERHOUT, Mark J. BURK, John D. TRAWICK, Michael P. KUCKINSKAS, Brian STEER
  • Publication number: 20140373192
    Abstract: A high efficiency plant expression promoter from Capsicum annuum serine hydroxymethyl transferase gene and uses thereof. This high efficiency plant expression promoter and 5?-untranslated region (5?-UTR) from Capsicum annular, serine hydroxymethyl transferase gene, a high efficiency plant expression vector having the same, a plant transformed with said vector, a process for high efficiency expression of a foreign gene by using said vector and a transformed plant which expresses with high efficiency a foreign gene based on said process and seeds of the transformed plant.
    Type: Application
    Filed: August 19, 2010
    Publication date: December 18, 2014
    Applicant: INDUSTRY FOUNDATION OF CHONNAM NATIONAL UNIVERSITY
    Inventors: Young Hee Joung, Do Il Choi, Sang Hyeob Lee
  • Publication number: 20140363864
    Abstract: A non-naturally occurring microbial organism includes a microbial organism having a 1,4-cyclohexanedimethanol pathway that includes at least one exogenous nucleic acid encoding a 1,4-cyclohexanedimethanol pathway enzyme expressed in a sufficient amount to produce 1,4-cyclohexanedimethanol. A method for producing 1,4-cyclohexanedimethanol includes culturing a non-naturally occurring microbial organism having a 1,4-cyclohexanedimethanol pathway. The pathway includes at least one exogenous nucleic acid encoding a 1,4-cyclohexanedimethanol pathway enzyme expressed in a sufficient amount to produce 1,4-cyclohexanedimethanol, under conditions and for a sufficient period of time to produce 1,4-cyclohexanedimethanol.
    Type: Application
    Filed: January 10, 2014
    Publication date: December 11, 2014
    Applicant: Genomatica, Inc.
    Inventors: Priti Pharkya, Mark J. Burk
  • Publication number: 20140356919
    Abstract: A non-naturally occurring microbial organism has cyclohexanone pathways that include at least one exogenous nucleic acid encoding a cyclohexanone pathway enzyme. A pathway includes a 2-ketocyclohexane-1-carboxyl-CoA hydrolase (acting on C—C bond), a 2-ketocyclohexane-1-carboxylate decarboxylase and an enzyme selected from a 2-ketocyclohexane-1-carboxyl-CoA hydrolase (acting on thioester), a 2-ketocyclohexane-1-carboxyl-CoA transferase, and a 2-ketocyclohexane-1-carboxyl-CoA synthetase.
    Type: Application
    Filed: February 4, 2014
    Publication date: December 4, 2014
    Inventors: Robin E. Osterhout, Anthony P. Burgard, Mark J. Burk, Priti Pharkya
  • Publication number: 20140329916
    Abstract: Provided herein is a non-naturally occurring microbial organism having a methanol metabolic pathway that can enhance the availability of reducing equivalents in the presence of methanol. Such reducing equivalents can be used to increase the product yield of organic compounds produced by the microbial organism, such as adipate, 6-aminocaproate, hexamethylenediamine or caprolactam. Also provided herein are methods for using such an organism to produce adipate, 6-aminocaproate, hexamethylenediamine or caprolactam.
    Type: Application
    Filed: December 16, 2013
    Publication date: November 6, 2014
    Applicant: Genomatica, Inc.
    Inventors: Anthony P. Burgard, Robin E. Osterhout, Stephen J. Van Dien, Cara Ann Tracewell, Priti Pharkya, Stefan Andrae
  • Publication number: 20140329250
    Abstract: The invention provides calcium-binding photoproteins which can detect light emission with a higher sensitivity. The proteins of the invention comprising the amino acid sequence of SEQ ID NO: 2 can be used for the detection and measurement of calcium ions. The proteins of the invention are useful as reporter proteins, luminescent markers, etc. The polynucleotides of the invention are useful as reporter genes, etc.
    Type: Application
    Filed: May 30, 2014
    Publication date: November 6, 2014
    Inventors: Satoshi INOUYE, Yuiko SAHARA
  • Publication number: 20140325709
    Abstract: The present invention relates to a process for the production of fine chemicals in a microorganism, a plant cell, a plant, a plant tissue or in one or more parts thereof. The present invention relates further to a process for the control of the production of fine chemicals in a microorganism, a plant cell, a plant, a plant tissue or in one or more parts thereof. The invention furthermore relates to nucleic acid molecules, polypeptides, nucleic acid constructs, vectors, antisense molecules, antibodies, host cells, plant tissue, propagation material, harvested material, plants, microorganisms as well as agricultural compositions and to their use.
    Type: Application
    Filed: September 22, 2011
    Publication date: October 30, 2014
    Applicant: Metanomics GmbH
    Inventors: Gunnar Plesch, Piotr Puzio, Astrid Blau, Ralf Looser, Birgit Wendel, Beate Kamlage, Oliver Schmitz
  • Patent number: 8852903
    Abstract: Recombinant microorganisms having an improved ability to co-metabolize in medium both fructose and glucose are described. The recombinant microorganisms comprise a promoter operably linked to a native or non-native nucleotide sequence that encodes a fucose: H+ symporter polypeptide. The recombinant microorganisms are useful for the production of a variety of products including glycerol and glycerol derivatives such as 1,3-propanediol and 3-hydroxypropionic acid.
    Type: Grant
    Filed: October 18, 2010
    Date of Patent: October 7, 2014
    Assignee: E I du Pont de Nemours and Company
    Inventors: Andrew C. Eliot, Anthony A. Gatenby, John S. Chapman
  • Publication number: 20140283202
    Abstract: The present disclosure relates, in some embodiments, to materials, systems, organisms, and methods for enhancing abiotic stress tolerance (e.g., cold, salinity, drought, heat, wind) and/or enhancing biomass in plants. For example, enhancing abiotic stress tolerance may be achieved in plants having Arabidopsis thaliana BCL-2-associated athanogene 4 (AtBAG4) nucleic acids and/or polypeptides, Caenorhabditis elegans Ced-9 nucleic acids and/or polypeptides, and/or human Bcl-2-161 nucleic acids and/or polypeptides.
    Type: Application
    Filed: March 14, 2014
    Publication date: September 18, 2014
    Applicant: THE TEXAS A&M UNIVERSITY SYSTEM
    Inventors: Martin B. Dickman, T. Erik Mirkov, Getu Beyene, Mayra Faion-Molina, Marco David Molina Risco
  • Patent number: 8835162
    Abstract: The invention provides a method of incorporating nonstandard amino acids into a protein by utilizing a modified aminoacyl-tRNA synthetase to charge the nonstandard amino acid to a modified tRNA, which forms strict Watson-Crick base-pairing with a codon that normally forms wobble base-pairing with natural tRNAs.
    Type: Grant
    Filed: December 28, 2012
    Date of Patent: September 16, 2014
    Assignee: California Institute of Technology
    Inventors: Inchan Kwon, David Tirrell
  • Patent number: 8809048
    Abstract: The object is to provide a technique for modifying a bacterium belonging to the genus Kocuria through genetic engineering for industrially effectively utilizing the bacterium belonging to the genus Kocuria. The above object can be achieved by providing a cyclic plasmid, which has a replication region comprising the base sequence of a DNA-binding protein-like protein gene, the base sequence of a replicase-like protein gene, and the base sequence represented by SEQ ID NO:45, and is autonomously replicable in bacteria. As an example of the aforesaid plasmid, a plasmid containing a base sequence represented by SEQ ID NO:3 or 4 which originates in Kocuria sp. MBE131 strain (FERM P-21885) can be cited.
    Type: Grant
    Filed: March 18, 2011
    Date of Patent: August 19, 2014
    Assignee: Independent Administrative Institution, Japan Agency for Marine-Earth Science and Technology
    Inventors: Yukari Ohta, Yuji Hatada, Kozue Mori, Nobuyuki Nakamura
  • Publication number: 20140223596
    Abstract: The present invention provides use of a plant gene JAZ5a for improving drought-resistance of a plant. It further provides a method for improving the drought-resistance of a plant, comprising enhancing the expression or activity of Jaz5A in said plant.
    Type: Application
    Filed: July 5, 2012
    Publication date: August 7, 2014
    Inventor: Adrianus Johannes Van Tunen
  • Publication number: 20140193856
    Abstract: The present invention relates generally to a method of modifying gene expression and to synthetic genes for modifying endogenous gene expression in a cell, tissue or organ of a transgenic organism, in particular a transgenic animal or plant. More particularly, the present invention utilizes recombinant DNA technology to post-transcriptionally modify or modulate the expression of a target gene in a cell, tissue organ or whole organism, thereby producing novel phenotypes. Novel synthetic genes and genetic constructs which are capable of repressing delaying or otherwise reducing the expression of an endogenous gene or target gene in an organism when introduced thereto are also provided.
    Type: Application
    Filed: December 20, 2013
    Publication date: July 10, 2014
    Applicant: Commonwealth Scientific and Industrial Research Organisation
    Inventors: Michael Wayne Graham, Robert Norman Rice, Peter Michael Waterhouse
  • Patent number: 8772576
    Abstract: Exemplary embodiments are directed to a herbicide resistant N2-fixing microorganism and to a method for enhancing N2 fixation by the herbicide resistant rhizobia in symbiosis with herbicide resistant or tolerant leguminous plants treated with herbicide. At least one preferred embodiment comprises mutant rhizobia strains selected based on tolerance of the broad spectrum glyphosate weed control agent, originally Roundup®. Preferred embodiments can be expected to outcompete both indigenous soil rhizobia and/or commercial rhizobia that are not resistant to the herbicide.
    Type: Grant
    Filed: September 3, 2010
    Date of Patent: July 8, 2014
    Inventor: L. David Kuykendall
  • Patent number: 8759070
    Abstract: The present invention relates a recombinant Clostridium expressing one or more heterologous Wood-Ljungdahl (WL) genes. In particular, the recombinant Clostridium produces a metabolite at an increased level. The present invention also relates to a method for producing a metabolite by the recombinant Clostridium.
    Type: Grant
    Filed: September 9, 2011
    Date of Patent: June 24, 2014
    Assignee: University of Delaware
    Inventors: Eleftherios T. Papoutsakis, Mohab Ali Al-Hinai, Shawn William Jones, Dinesh Chanukya Indurthi, Daniel Knox Mitchell, Alan Fast
  • Patent number: 8759616
    Abstract: The present invention relates to methods and compositions for improving the efficiency of Agrobacterium- and Rhizobium-mediated plant cell transformation by use of additional transformation enhancer sequences, such as overdrive or TSS sequences, operably linked to a T-DNA border sequence on a recombinant construct that comprises T-DNA.
    Type: Grant
    Filed: March 19, 2013
    Date of Patent: June 24, 2014
    Assignee: Monsanto Technology LLC
    Inventors: Larry A. Gilbertson, Xudong Ye
  • Publication number: 20140143911
    Abstract: The present invention relates to the field of plant genetic engineering. More specifically, the present invention relates to seed specific gene expression during a defined period of embryogenesis. The present invention provides promoters capable of transcribing heterologous nucleic acid sequences in seeds, and methods of modifying, producing, and using the same.
    Type: Application
    Filed: November 12, 2013
    Publication date: May 22, 2014
    Applicant: MONSANTO TECHNOLOGY LLC
    Inventors: QI WANG, LISA M. WEAVER, TIM N. ULMASOV, JEFFREY E. AHRENS, PATRICE DUBOIS, JEFFERY QINGXI SHEN