Rhizobium Or Agrobacterium Patents (Class 435/252.2)
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Publication number: 20130109069Abstract: The invention provides non-naturally occurring microbial organisms comprising a 1,4-butanediol (BDO) pathway comprising at least one exogenous nucleic acid encoding a BDO pathway enzyme expressed in a sufficient amount to produce BDO. The invention additionally provides methods of using such microbial organisms to produce BDO.Type: ApplicationFiled: April 17, 2012Publication date: May 2, 2013Applicant: GENOMATICA, INC.Inventors: Mark J. Burk, Anthony P. Burgard, Robin E. Osterhout, Jun Sun
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Publication number: 20130109064Abstract: The invention provides non-naturally occurring microbial organisms containing 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol pathways comprising at least one exogenous nucleic acid encoding a butadiene pathway enzyme expressed in a sufficient amount to produce 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol. The invention additionally provides methods of using such microbial organisms to produce 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol, by culturing a non-naturally occurring microbial organism containing 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol pathways as described herein under conditions and for a sufficient period of time to produce 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol.Type: ApplicationFiled: August 17, 2012Publication date: May 2, 2013Inventors: Robin E. Osterhout, Anthony P. Burgard, Mark J. Burk
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Publication number: 20130102035Abstract: The present invention relates to combinatorial variants of a parent glucoamylase that have altered properties for reducing the synthesis of condensation products during hydrolysis of starch. Accordingly the variants of a parent glucoamylase are suitable such as for use within brewing and glucose syrup production. Also disclosed are DNA constructs encoding the variants and methods of producing the glucoamylase variants in host cells.Type: ApplicationFiled: June 30, 2011Publication date: April 25, 2013Applicant: DUPONT NUTRITION BIOSCIENCES APSInventors: Peter Edvard Degn, Richard R. Bott, Casper Willem Vroemen, Martijn Silvan Scheffers, Wolfgang Aehle, Elin Petersen
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Publication number: 20130102664Abstract: An acetyl xylan esterase variant having perhydrolytic activity is provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. More specifically, a Thermotoga maritima acetyl xylan esterase gene was modified using error-prone PCR and site-directed mutagenesis to create an enzyme catalyst characterized by an increase in specific activity. The variant acetyl xylan esterase may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, and paper pulp processing applications.Type: ApplicationFiled: October 22, 2012Publication date: April 25, 2013Applicant: E. I. DU PONT DE NEMOURS AND COMPANYInventor: E. I. du Pont de Nemours and Company
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Publication number: 20130102666Abstract: An acetyl xylan esterase variant having perhydrolytic activity is provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. More specifically, a Thermotoga maritima acetyl xylan esterase gene was modified using error-prone PCR and site-directed mutagenesis to create an enzyme catalyst characterized by an increase in specific activity. The variant acetyl xylan esterase may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, and paper pulp processing applications.Type: ApplicationFiled: October 22, 2012Publication date: April 25, 2013Applicant: E. I. DU PONT DE NEMOURS AND COMPANYInventor: E. I. Du Pont De Nemours And Company
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Publication number: 20130102672Abstract: An acetyl xylan esterase variant having perhydrolytic activity is provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. More specifically, a Thermotoga maritima acetyl xylan esterase gene was modified using error-prone PCR and site-directed mutagenesis to create an enzyme catalyst characterized by an increase in specific activity. The variant acetyl xylan esterase may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, and paper pulp processing applications.Type: ApplicationFiled: October 22, 2012Publication date: April 25, 2013Applicant: E. I. DU PONT DE NEMOURS AND COMPANYInventor: E.I. Du Pont De Nemours And Company
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Publication number: 20130095540Abstract: The invention provides a non-naturally occurring microbial organism having an adipate, 6-aminocaproic acid or caprolactam pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in the respective adipate, 6-aminocaproic acid or caprolactam pathway. The invention additionally provides a method for producing adipate, 6-aminocaproic acid or caprolactam. The method can include culturing an adipate, 6-aminocaproic acid or caprolactam producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding an adipate, 6-aminocaproic acid or caprolactam pathway enzyme in a sufficient amount to produce the respective product, under conditions and for a sufficient period of time to produce adipate, 6-aminocaproic acid or caprolactam.Type: ApplicationFiled: June 15, 2012Publication date: April 18, 2013Applicant: Genomatica, Inc.Inventors: Anthony P. BURGARD, Priti Pharkya, Robin E. Osterhout
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Publication number: 20130084601Abstract: The present invention relates to the novel Rhizobium sp. GIN611 KCTC11708BP or to cell extracts thereof, to a novel oxidoreductase which exhibits a glycolytic activity, to a gene encoding the oxidoreductase, to a recombinant strain comprising recombinant vector proteins or to an expression vector encoding recombinant proteins, and to a method for the glycolysis of natural products using same as a biocatalyst. The present invention also relates to a method for producing aglycones from a variety of natural products using same. The novel oxidoreductase separated from the novel microorganism of the present invention does not belong to a glucosidase group but belongs to an oxidoreductase group, and has a glycolytic activity for natural products. The novel oxidoreductase oxidizes the sugar in the aglycones of natural products, thereby producing a variety of aglycones.Type: ApplicationFiled: June 14, 2011Publication date: April 4, 2013Applicant: AMOREPACIFIC CORPORATIONInventors: Jun Seong Park, Duck Hee Kim, Han Kon Kim, Eun Mi Kim, Byung Gee Kim
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Publication number: 20130085268Abstract: The invention provides a composition for the inoculation in a plant of agrobacteria transfected by expression vectors, in order to produce in said plant a protein of interest or a derivative of said protein, by deletion or by mutation, characterised in that it comprises agrobacteria transfected by at least one expression vector, comprising a nucleotide sequence insert that codes for said protein or a derivative of said protein, and agrobacteria transfected by a plurality of expression vectors, each comprising at least one nucleotide sequence insert that codes for proteins having a silencing suppressor effect.Type: ApplicationFiled: May 26, 2011Publication date: April 4, 2013Applicants: Institut de L'Environnement et de Recherches Agricoles, Institut de Recherche pour le Developpement (IRD)Inventors: Christophe Brugidou, Jean-Loup Lemesre, Denis Fargette, Drissa Sereme, Moumouni Konate, Gnissa Konate
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Patent number: 8404825Abstract: In various embodiments, the present disclosure provides a method and enzyme for forming various compounds, such as monoterpenes and monoterpenoid compounds. In a specific example, the present disclosure provides a method for producing one or more of (?)-ipsdienol, (?)-ipsenol, ipsenone, and ipsdienone. The present disclosure also provides methods of using compounds formed from the disclosed method and enzyme.Type: GrantFiled: November 17, 2009Date of Patent: March 26, 2013Assignee: Board of Regents of the Nevada System of Higher Education, on Behalf of the University of Nevada, RenoInventors: Claus Tittiger, Rubi Figueroa-Teran, Gary J. Blomquist
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Patent number: 8404931Abstract: The present invention relates to methods and compositions for improving the efficiency of Agrobacterium- and Rhizobium-mediated plant cell transformation by use of additional transformation enhancer sequences, such as overdrive or TSS sequences, operably linked to a T-DNA border sequence on a recombinant construct that comprises T-DNA.Type: GrantFiled: February 25, 2011Date of Patent: March 26, 2013Assignee: Monsanto Technology LLCInventors: Larry Gilbertson, Xudong Ye
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Publication number: 20130071886Abstract: The invention provides non-naturally occurring microbial organisms comprising a 1,4-butanediol (BDO) pathway comprising at least one exogenous nucleic acid encoding a BDO pathway enzyme expressed in a sufficient amount to produce BDO. The invention additionally provides methods of using such microbial organisms to produce BDO.Type: ApplicationFiled: August 3, 2012Publication date: March 21, 2013Applicant: GENOMATICA, INC.Inventors: Mark J. Burk, Anthony P. Burgard, Robin E. Osterhout, Jun Sun
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Publication number: 20130071883Abstract: The invention provides a non-naturally occurring microbial organism having an acetyl-CoA pathway and the capability of utilizing syngas or syngas and methanol. In one embodiment, the invention provides a non-naturally occurring microorganism, comprising one or more exogenous proteins conferring to the microorganism a pathway to convert CO, CO2 and/or H2 to acetyl-coenzyme A (acetyl-CoA), methyl tetrahydrofolate (methyl-THF) or other desired products, wherein the microorganism lacks the ability to convert CO or CO2 and H2 to acetyl-CoA or methyl-THF in the absence of the one or more exogenous proteins.Type: ApplicationFiled: September 13, 2012Publication date: March 21, 2013Applicant: GENOMATICA, INC.Inventors: Mark J. BURK, Christophe H. SCHILLING, Anthony P. BURGARD, John D. TRAWICK
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Patent number: 8399737Abstract: The invention relates to a resistance gene and functional homologs or fragments thereof isolated from Solanum bulbocastanum. Moreover, the invention relates to the use of this resistance gene, for example, in a method to increase or confer at least partial resistance in a plant to an oomycete infection.Type: GrantFiled: January 28, 2008Date of Patent: March 19, 2013Assignees: Wageningen Universiteit, Kweek—en Researchbedrijf Agrico B.V.Inventors: Edwin Andries Gerard Van Der Vossen, Anoma Akuvi Lokossou, Richard Gerardus Franciscus Visser, Evert Jacobsen
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Publication number: 20130065279Abstract: The invention provides a non-naturally occurring microbial organism having a methacrylic acid, methacrylate ester, 3-hydroxyisobutyrate and/or 2-hydroxyisobutyrate pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in a methacrylic acid pathway. The invention additionally provides a method for producing methacrylic acid, methacrylate ester, 3-hydroxyisobutyrate and/or 2-hydroxyisobutyrate. The method can include culturing methacrylic acid, methacrylate ester, 3-hydroxyisobutyrate and/or 2-hydroxyisobutyrate producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding a methacrylic acid pathway enzyme in a sufficient amount to produce methacrylic acid, methacrylate ester, 3-hydroxyisobutyrate and/or 2-hydroxyisobutyrate, under conditions and for a sufficient period of time to produce methacrylic acid, methacrylate ester, 3-hydroxyisobutyrate and/or 2-hydroxyisobutyrate.Type: ApplicationFiled: March 30, 2012Publication date: March 14, 2013Applicant: Genomatica, Inc.Inventors: Mark J. BURK, Anthony P. Burgard, Robin E. Osterhout, Jun Sun, Priti Pharkya
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Publication number: 20130065284Abstract: A modified microorganism for high efficient production of lactic acid, an expression vector for constructing the modified microorganism, and a method of producing a lactic acid using the same are disclosed. The modified microorganism may produce lactic acid at a high level under acid conditions.Type: ApplicationFiled: June 22, 2012Publication date: March 14, 2013Applicant: SAMSUNG ELECTRONICS CO., LTD.Inventors: Soon Chun CHUNG, Hyun Min KOO, Jae Young KIM, Ji Eun KIM, Jin Woo KIM, Young kyoung PARK, So Young LEE, Hwa Young CHO, Dae Huck KWON, Jae Chan PARK
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Publication number: 20130052700Abstract: The invention provides a biocatalytic process for oxidation of organic compounds with the aid of an alkL gene product, and microorganisms used in this process.Type: ApplicationFiled: March 15, 2011Publication date: February 28, 2013Inventors: Markus Poetter, Andreas Schmid, Bruno Buehler, Hans-Georg Hennemann, Mattijs Kamiel Julsing, Steffen Schaffer, Thomas Haas, Manfred Schrewe, Sjef Cornelissen, Martin Roos, Harald Haeger
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Publication number: 20130045323Abstract: The present invention relates to methods for increasing the protein, oil, and/or amino acid content of a plant. The methods involve the manipulation of the expression level of trehalose-6-phosphate synthase (TPS) homologs. Expression cassettes for achieving such gene expression manipulation and transgenic plant cells and plants comprising the constructs and cassettes are also provided. Methods of plant breeding using these plants have also been developed.Type: ApplicationFiled: August 16, 2012Publication date: February 21, 2013Applicant: BASF PLANT SCIENCE COMPANY GMBHInventor: Heiko Härtel
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Patent number: 8372601Abstract: The disclosure of the present application provides polypeptide sequences and nucleotide sequences coding for the polypeptide sequences of proteins used in the production of APPA-containing peptides. In at least one embodiment of the present disclosure, an isolated nucleic acid is disclosed which comprises a nucleotide sequence encoding a polypeptide having a sequence identity of 60 percent or greater to an amino acid sequence selected from the group consisting of SEQ ID NOS: 2-13, and 15-23.Type: GrantFiled: January 21, 2011Date of Patent: February 12, 2013Assignee: University of Illinois at Urbana-ChampaignInventors: William W. Metcalf, Wilfred A. van der Donk, Junkal Zhang, Benjamin T. Circello, Svetlana A. Borisova
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Patent number: 8367392Abstract: A method for transforming algal or cyanobacterial cells, comprising mixing a polynucleotide for transforming the cells with the polynucleotide; performing microporation by applying a plurality of electrical pulses to the cells with a microporation apparatus; and incubating said polynucleotide with the cells after said applying said electrical pulses.Type: GrantFiled: September 8, 2009Date of Patent: February 5, 2013Assignee: Transalgae Ltd.Inventors: Daniella Schatz, Doron Eisenstadt, Jonathan Gressel, Shai Ufaz, Ofra Chen, Dikla Journo Eckstein
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Publication number: 20130029381Abstract: The invention provides non-naturally occurring microbial organisms comprising a 1,4-butanediol (BDO), 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine pathway comprising at least one exogenous nucleic acid encoding a BDO, 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine pathway enzyme expressed in a sufficient amount to produce BDO, 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine and further optimized for expression of BDO. The invention additionally provides methods of using such microbial organisms to produce BDO, 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine.Type: ApplicationFiled: April 13, 2012Publication date: January 31, 2013Applicant: GENOMATICA, INC.Inventors: Robert Haselbeck, John D. Trawick, Wei Niu, Anthony P. Burgard
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Publication number: 20130017583Abstract: The invention relates to the production of polyhydroxyalkanoate copolymer with high 3-hydroxyhexanoate monomer content through recombinant gene expression.Type: ApplicationFiled: November 10, 2010Publication date: January 17, 2013Applicant: Massachusetts Institute of TechnologyInventors: Charles Forrester Budde, Chokyun Rha
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Patent number: 8354246Abstract: The invention provides a method of inhibiting a bacterial RNA polymerases. The invention has applications in control of bacterial RNA polymerase activity, control of bacterial gene expression, control of bacterial growth, antibacterial chemistry, and antibacterial therapy.Type: GrantFiled: December 18, 2009Date of Patent: January 15, 2013Assignee: Rutgers, The State University of New JerseyInventors: Richard H. Ebright, Jayanta Mukhopadhyay, Konstantin Severinov, Ekaterina Semenova
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Patent number: 8354265Abstract: The present invention concerns the field of bacterial biocontrol. More precisely, the invention relates to the identification of chemicals which promote the growth of bacteria inactivating NAHL, such as gamma-caprolactone (GCL) and 4-heptanolide (HTN) and their use in soil additives.Type: GrantFiled: January 18, 2008Date of Patent: January 15, 2013Assignees: Centre National de la Recherche Scientifique, Comite Economique Agricole Production des Plants du Nord (Comite Nord Pomme de Terre)Inventors: Denis Faure, Amélie Cirou, Yves Dessaux
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Publication number: 20130011891Abstract: The invention provides non-naturally occurring microbial organisms having a butadiene or crotyl alcohol pathway. The invention additionally provides methods of using such organisms to produce butadiene or crotyl alcohol.Type: ApplicationFiled: June 19, 2012Publication date: January 10, 2013Inventors: Mark J. Burk, Anthony P. Burgard, Robin E. Osterhout, Jun Sun, Priti Pharkya
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Publication number: 20130012437Abstract: Lytic peptides, including fusion peptides of lytic peptides conjugated with luteinizing hormone-releasing hormone or modified versions thereof to target luteinizing hormone-releasing hormone receptors, are disclosed. The lytic peptides show anti-proliferative activity against human prostate cancer cell lines, but are nontoxic to normal primary human prostate epithelial cells or to bone marrow stromal cells in co-culture. The lytic peptides have specificity for and anti-proliferative activity against prostate cancer tumor cells, and low toxicity for normal prostate cells, making the peptides useful in therapies for prostate cancer.Type: ApplicationFiled: July 7, 2011Publication date: January 10, 2013Applicant: Tuskegee UniversityInventors: Clayton YATES, Jesse JAYNES, Timothy TURNER
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Publication number: 20120329119Abstract: The invention provides non-naturally occurring microbial organisms having a propylene pathway. The invention additionally provides methods of using such organisms to produce propylene.Type: ApplicationFiled: June 19, 2012Publication date: December 27, 2012Inventors: Anthony P. Burgard, Robin E. Osterhout, Jun Sun, Priti Pharkya, Mark J. Burk
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Publication number: 20120331579Abstract: The invention relates to a reversible transgenic plant male sterility system wherein the male sterility is induced by amiRNA and a reversible transgenic plant male sterility system comprising a male sterility construct comprising an isolated nucleic acid encoding a precursor amiRNA encoding an amiRNA targeted to a gene involved in pollen development, and a male fertility restorer construct comprising an isolated nucleic acid encoding a mutated copy of the gene involved in pollen development, or multiple copies of said gene involved in pollen development, or a single copy of said gene involved in pollen development under the control of a strong promoter.Type: ApplicationFiled: November 11, 2010Publication date: December 27, 2012Applicant: La TROBE UNIVERSITYInventors: Roger Parish, Song Feng Li
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Publication number: 20120331589Abstract: The subject invention includes methods and plants for controlling lepidopteran insects, said plants comprising Cry1Fa and Cry1Da core toxin containing proteins in combination to delay or prevent development of resistance by the insect(s).Type: ApplicationFiled: December 16, 2010Publication date: December 27, 2012Applicant: DOW AGROSCIENCES LLCInventors: Thomas Meade, Kenneth Narva, Nicholas P. Storer, Joel J. Sheets, Aaron T. Woosley, Stephanie L. Burton
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Publication number: 20120329104Abstract: A modified microorganism having enhanced xylose utilization, an expression vector for constructing the modified microorganism, and a method of producing a chemical using the same are disclosed.Type: ApplicationFiled: June 27, 2012Publication date: December 27, 2012Applicants: SEOUL UNIVERSITY RESEARCH AND BUSINESS FOUNDATION, SAMSUNG ELECTRONICS CO., LTD.Inventors: Jae Young KIM, Hyun Min KOO, Young kyoung PARK, Jae Chan PARK, Sung Min PARK, Jin Ho SEO, Yong Cheol PARK
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Patent number: 8338163Abstract: The present invention provides a triple vital bacteria composition, including: powder of Bifidobacterium longum, powder of Lactobacillus acidophilus, and powder of Streptococcus faecalis. The present invention further provides a method for preparing the above triple vital bacteria composition, as well as the protection agents, fermenting culture medium and seed culture medium used therein.Type: GrantFiled: February 3, 2008Date of Patent: December 25, 2012Assignee: Shanghai Sine Pharmaceutical Laboratories Co., Ltd.Inventors: Binhua Chen, Xuesong Wang, Liang Zhang
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Patent number: 8334139Abstract: This invention relates to several plasmids which are useful for genetically transforming plant cells. A first plasmid, such as pMON120, contains a T-DNA border, one or more marker genes, a unique cleavage site, and a region of Ti plasmid homology. A gene which is expressed in plant cells may be inserted into this plasmid to obtain a derivative plasmid, such as pMON128 which expresses neomycin phosphotransferase in plant cells. The derivative plasmid is inserted into a suitable microorganism, such as A. tumefaciens which contains a Ti plasmid. The inserted plasmids recombine with Ti plasmids to form co-integrate plasmids. Only a single crossover event is required to create the desired co-integrate plasmid. A. tumefaciens cells with co-integrate plasmids are selected and co-cultured with plant cells. The co-integrate Ti plasmids enter the plant cells and insert a segment of T-DNA which does not contain tumorigenic genes into the plant genome.Type: GrantFiled: October 4, 1985Date of Patent: December 18, 2012Assignee: Monsanto Technology LLCInventors: Robert T. Fraley, Stephen G. Rogers
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Patent number: 8334429Abstract: Auxotrophic Agrobacterium and methods employing auxotrophic Agrobacterium are provided. Auxotrophic Agrobacterium may be used in a variety of methods including biologically containing Agrobacterium comprising a transgene and transforming a plant cell without using an Agrobacterium counter-selective agent. Transforming maize immature embryos using an Agrobacterium auxotrophic for thymidine results in comparable transformation efficiency as transformation achieved using prototrophic Agrobacterium. Methods for producing an Agrobacterium thymidine auxotroph and using the auxotroph in transformation methods are disclosed. Transformed tissues and plants produced using methods of the present invention are also provided.Type: GrantFiled: December 30, 2009Date of Patent: December 18, 2012Assignee: Pioneer Hi Bred International IncInventors: Jerome P. Ranch, Matthias Liebergesell, Carl W. Garnaat, Gary A. Huffman
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Patent number: 8334428Abstract: Methods and compositions reduce copy number of transgenes and minimizing vector backbone sequences in plant transformation. Agrobacterium strains with T-DNA integrated into the chromosomal DNA of the Agrobacterium reduce the copy number and vector backbone sequences. Chromosomal integration vectors to integrate T-DNA into a specific locus of Agrobacterium chromosome are disclosed.Type: GrantFiled: November 14, 2007Date of Patent: December 18, 2012Assignees: Purdue Research Foundation, Iowa State University Research Foundation, Inc.Inventors: Stanton B. Gelvin, Heiko Oltmanns, Lan Ying Lee, Bronwyn Frame, Kan Wang
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Publication number: 20120316101Abstract: The present invention relates to a polypeptide capable of specifically targeting apoptotic cells undergoing apoptosis and a use thereof. More particularly, it relates to an isolated polypeptide consisting of the sequence (I): Cys-X1-Val-Ala-Pro-X2 (I), wherein X1 is an amino acid with polar uncharged side chain and X2 is an amino acid with positive charged side chain and targeting apoptotic cells, a composition for detection of apoptotic cells comprising the same as an effective ingredient, a composition for drug delivery comprising the same as an effective ingredient, a composition for imaging comprising the same as an effective ingredient and the like. Accordingly, the peptide of the present invention may be useful for detection of apoptotic cells, as well as detection and imaging of apoptotic cells in tumor tissue, apoptotic myocardial cells in myocardial infarction tissue, apoptotic nerve cells in stroke tissue and arteriosclerosis site, and targeted drug delivery thereto.Type: ApplicationFiled: May 9, 2012Publication date: December 13, 2012Applicant: KYUNGPOOK NATIONAL UNIVERSITY INDUSTRY-ACADEMIC COOPERATION FOUNDATIONInventors: Byung Heon Lee, In San Kim
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Publication number: 20120317679Abstract: The present invention relates to the field of plant genetic engineering. More specifically, the present invention relates to seed specific gene expression during a defined period of embryogenesis. The present invention provides promoters capable of transcribing heterologous nucleic acid sequences in seeds, and methods of modifying, producing, and using the same.Type: ApplicationFiled: April 4, 2012Publication date: December 13, 2012Inventors: Qi Wang, Lisa M. Weaver, Tim N. Oulmassov, Jeffrey Ahrens, Patrice Dubois, Jeffery Q. Shen
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Publication number: 20120317682Abstract: The subject invention includes methods and plants for controlling lepidopteran insects, said plants comprising a Vip3Ab insecticidal protein in combination with a Cry 1Fa insecticidal protein to delay or prevent development of resistance by the insect(s).Type: ApplicationFiled: December 16, 2010Publication date: December 13, 2012Applicant: Dow Agrosciences LLCInventors: Thomas Meade, Kenneth Narva, Nicholas P. Storer, Joel J. Sheets, Aaron T. Woosley, Stephanie L. Burton
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Publication number: 20120317681Abstract: The subject invention includes methods and plants for controlling lepidopteran insects, said plants comprising a Cry1Fa insecticidal protein and a Cry1Ca insecticidal protein, in combination, to delay or prevent development of resistance by the insect(s).Type: ApplicationFiled: December 16, 2010Publication date: December 13, 2012Inventors: Thomas Meade, Kenneth Narva, Nicholas P. Storer, Joel J. Sheets, Aaron T. Woosley, Stephanie L. Burton
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Publication number: 20120311745Abstract: The subject invention includes methods and plants for controlling lepidopteran insects, said plants comprising Cry1Ca insecticidal protein and a Cry1Ab insecticidal protein in combination to delay or prevent development of resistance by the insects.Type: ApplicationFiled: December 16, 2010Publication date: December 6, 2012Applicant: DOW AGROSCIENCES LLCInventors: Thomas Meade, Aaron T. Woosley, Kenneth Narva, Stephanie L. Burton, Nicholas P. Storer, Joel J. Sheets
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Publication number: 20120311746Abstract: The subject invention relates in part to stacking Cry1Be toxins along with Cry1Fa toxins to prevent insects from developing resistance towards either toxin by itself. As discussed in more detail herein, the subject pair of proteins is a particularly advantageous combination, as no other pair of proteins is known to provide high levels of control and non-cross-resistant activity against both Spodoptera frugiperda (FAW) and Ostrinia nubilalis (ECB) insects. This dual, non-cross-resistant activity is also advantageous because it can reduce the number of proteins/genes needed to target these insects with multiple, non-cross-resistant proteins. This can reduce or eliminate the need for refuge acreage. Accordingly, the subject invention also relates generally to using four genes to provide three proteins for non-cross-resistant control of a first insect, and three proteins for non-cross-resistant control of a second insect. In preferred embodiments, the targeted insects are FAW and ECB.Type: ApplicationFiled: December 16, 2010Publication date: December 6, 2012Applicant: Dow Agroscience LLCInventors: Thomas Meade, Kenneth Narva, Nicholas P. Storer, Joel J. Sheets, Stephanie L. Burton, Aaron T. Woosley
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Publication number: 20120309918Abstract: The invention relates to a polypeptide having oxidoreductase activity which comprises the amino acid sequence set out in SEQ ID NO: 3 or an amino acid sequence encoded by the nucleotide sequence of SEQ ID NO: 4, or a variant polypeptide thereof having 45% or more sequence identity with the sequence of SEQ ID NO: 3. The invention also relates to a process for the production of 2,5-furan-dicarboxylic acid (FDCA) or production of 5-hydroxymethyl-2-furancarboxylic acid (HMF acid).Type: ApplicationFiled: September 2, 2010Publication date: December 6, 2012Applicant: DSM IP ASSETS B.V.Inventors: Harald Johan Ruijssenaars, Nick Johannes Petrus Wierckx, Frank Wouter Koopman, Adrianus Johannes Jozef Straathof, Johannes Hendrik De Winde
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Publication number: 20120304337Abstract: The invention provides novel ?-endotoxin polypeptides, and variants and fragments thereof, obtained from Bacillus thuringiensis having pesticidal activity against nematodes. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, biopesticide compositions, expression cassettes, and transformed microorganisms and plants comprising a nucleic acid of the invention. These compositions find use in methods for controlling pests, especially plant parasitic nematodes.Type: ApplicationFiled: May 23, 2012Publication date: November 29, 2012Applicant: Pioneer Hi-Bred International, Inc.Inventors: Ute Schellenberger, Jun-Zhi Wei, Genhai Zhu
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Publication number: 20120272406Abstract: The present invention relates to methods for increasing digestibility and/or crude protein of a plant by modifying lignin biosynthesis of the plant. The methods involve the manipulation of the expression level of genes in the lignin biosynthesis pathway by directly reducing the expression of genes using miRNA or using regulation of transcription factors. Expression cassettes for achieving such gene expression manipulation and transgenic plant cells and plants comprising the constructs and cassettes are also provided. Transcription regulating nucleotide sequences for use in the expression cassettes have also been developed as well as methods of using these sequences and cassettes.Type: ApplicationFiled: March 29, 2012Publication date: October 25, 2012Applicant: BASF Plant Science Company GmbHInventors: Hanping Guan, Beomseok Seo, Peifeng Ren, David Deppong, Huihua Fu
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Publication number: 20120272409Abstract: The present invention relates to two new gain of function variants of the cytokinin receptor proteins AHK2 and AHK3, namely rock2 and rock3, to transgenic organisms comprising at least one of said new gain-of-function cytokinin receptor variants and to a method for the manufacturing of a transgenic plant comprising at least one of the new gain-of-function variants.Type: ApplicationFiled: July 9, 2010Publication date: October 25, 2012Inventors: Thomas Schmülling, Tomás Werner, Isabel Bartrina Y Manns, Helen Braun
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Publication number: 20120264179Abstract: The invention provides a non-naturally occurring microbial organism having an adipate, 6-aminocaproic acid or caprolactam pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in the respective adipate, 6-aminocaproic acid or caprolactam pathway. The invention additionally provides a method for producing adipate, 6-aminocaproic acid or caprolactam. The method can include culturing an adipate, 6-aminocaproic acid or caprolactam producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding an adipate, 6-aminocaproic acid or caprolactam pathway enzyme in a sufficient amount to produce the respective product, under conditions and for a sufficient period of time to produce adipate, 6-aminocaproic acid or caprolactam.Type: ApplicationFiled: April 20, 2012Publication date: October 18, 2012Applicant: GENOMATICA, INC.Inventors: Anthony P. Burgard, Priti Pharkya, Robin E. Osterhout
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Publication number: 20120255071Abstract: The invention provides nucleic acids obtained from strains of Bacillus thuringiensis encoding ?-endotoxins having pesticidal activity against insect pests including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acid molecules encoding mutant pesticidal polypeptides, pesticidal compositions, expression cassettes, and transformed microorganisms and plants comprising a nucleic acid molecule of the invention. Such compositions find use in methods for controlling plant pests.Type: ApplicationFiled: March 30, 2012Publication date: October 4, 2012Applicant: Universidad Nacional Autonoma De MéxicoInventors: Mario Soberón-Chavez, Alejandra Bravo-De La Parra, Isabel Gómez-Gómez
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Publication number: 20120244621Abstract: The invention relates to bacterial promoter elements which constitute or which are comprised in promoter regions and which confer tumour specificity to the promoter region activity, resulting in transcription of a transgene, which can be a heterologous or a homologous gene, which transgene is functionally arranged downstream of the promoter region at presence of a host bacterium in tumour tissue, while essentially conferring inactivity of the promoter region and no transcription at presence of the host bacterium in non-tumour tissue. Accordingly, the invention relates to bacterial promoter regions containing these tumour specific promoter elements.Type: ApplicationFiled: May 28, 2010Publication date: September 27, 2012Applicant: HELMHOLTZ-ZENTRUM FUR INFEKTIONSFORSCHUNG GmbHInventors: Siegfried Weiss, Sara Bartels, Holger Loessner, Igor Deyneko, Dirk Bumann, Kathrin Westphal-Daniel
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Publication number: 20120246759Abstract: The present invention relates to the field of the production of transgenic plants through Agrobacterium-mediated transformation of cells of somatic embryogenic calli or embryogenic suspension cultures and regeneration of the transformed cells into fruit-setting plants. In particular, the present invention relates to the production of transgenic plants in the Euphorbiaceae family. The present invention further relates to media compositions, selection methods and engineered Agrobacterium tumefaciens strains that improve Agrobacterium-mediated transformation efficiency.Type: ApplicationFiled: December 4, 2009Publication date: September 27, 2012Applicant: TEMASEK LIFE SCIENCES LABORATORY LIMITEDInventors: Lin Cai, Longhua Sun, Lin Fu, Lianghui Ji
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Publication number: 20120237991Abstract: The present invention relates to novel mutants with cyclase activity and use thereof in a method for biocatalytic cyclization of terpenes, such as in particular for the production of isopulegol by cyclization of citronellal; a method for the preparation of menthol and methods for the biocatalytic conversion of further compounds with structural motifs similar to terpene.Type: ApplicationFiled: November 16, 2011Publication date: September 20, 2012Applicant: BASF SEInventors: MICHAEL BREUER, BERNHARD HAUER, DIETER JENDROSSEK, GABRIELE SIEDENBURG, JUERGEN PLEISS, DEMET SIRIM, SILVIA RACOLTA
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Publication number: 20120240284Abstract: The invention relates to a resistance gene and functional homologues or fragments thereof isolated from S. chacoense, S. berthaultii, S. sucrense or S. tarijense. More over, the invention relates to the use of said resistance gene, for example the use of said resistance gene in a method to increase or confer at least partial resistance in a plant to an oomycete infection. The invention provides an isolated or recombinant nucleic acid sequence comprising a nucleic acid sequence encoding one of the amino acid sequences of FIG. 4 or a functional fragment or a functional homologue thereof such as those presented in FIG. 13.Type: ApplicationFiled: September 20, 2010Publication date: September 20, 2012Applicant: WAGENINGEN UNIVERSITEITInventors: Jacobus Hubertus Vossen, Maarten Nijenhuis, Marion Johanna Barbara Arens-De Reuver, Edwin Andries Gerard Van Der Vossen, Evert Jacobsen, Richard Gerardus Franciscus Visser