Brevibacterium Or Corynebacterium Patents (Class 435/252.32)
  • Publication number: 20150147800
    Abstract: The present invention relates to a method for the enzymatic decarboxylation of malonic acid (propanedioic acid) derivatives catalyzed by enzymes structurally and/or functionally related to arylmalonate decarboxylase (AMDase) as isolated from microorganisms of the genus Bordetella. The present invention also relates to novel enzymes with a decarboxylase activity, useful for performing the claimed method, mutants thereof, corresponding coding sequences and expression systems, methods of preparing said novel enzymes, and screening methods for obtaining further suitable enzymes also having said decarboxylase activity.
    Type: Application
    Filed: December 29, 2014
    Publication date: May 28, 2015
    Inventors: Bernhard Hauer, Nina Schneider, Krzysztof Okrasa, Jason Micklefield, David Leys, Colin Levy
  • Publication number: 20150140631
    Abstract: Described is a method for generating conjugated dienes through a biological process. More specifically, the application describes a method for producing conjugated dienes (for example butadiene, isoprene or dimethylbutadiene) from light alkenols via enzymatic dehydration, in particular by making use of an alkenol dehydratase.
    Type: Application
    Filed: January 28, 2015
    Publication date: May 21, 2015
    Inventor: Philippe Marliere
  • Patent number: 9029104
    Abstract: The present invention provides a method for producing an L-amino acid belonging to the glutamate family, using a coryneform bacterium which has been modified so that expression of one or more gene(s) of the NCgl_2067-NCgl_2065 operon in said bacterium is/are attenuated.
    Type: Grant
    Filed: October 3, 2013
    Date of Patent: May 12, 2015
    Assignee: Ajinomoto Co., Inc.
    Inventors: Svetlana Alekseevna Samsonova, Viktor Vasilievich Samsonov, Yulia Georgievna Rostova, Mikhail Markovich Gusyatiner
  • Publication number: 20150125919
    Abstract: A recombinant microorganism having increased iron-regulated ABC transporter activity and increased hydroxycarboxylic acid production, as well as a method of producing a hydroxycarboxylic acid using the recombinant microorganism, and a method of producing the recombinant microorganism.
    Type: Application
    Filed: November 5, 2014
    Publication date: May 7, 2015
    Inventors: Hwayoung Cho, Jinhwan Park, Hongsoon Rhee, Kwangmyung Cho
  • Publication number: 20150111262
    Abstract: This document describes biochemical pathways for producing glutaric acid, 5-aminopentanoic acid, 5-hydroxypentanoic acid, cadaverine or 1,5-pentanediol by forming one or two terminal functional groups, comprised of carboxyl, amine or hydroxyl group, in a C5 backbone substrate such as D-proline.
    Type: Application
    Filed: August 27, 2014
    Publication date: April 23, 2015
    Inventors: Adriana Leonora Botes, Alex Van Eck Conradie
  • Publication number: 20150104837
    Abstract: The present invention relates to a novel isolated gene (polynucleotide) which encodes a protein having a biofilm formation inhibitory activity derived from Coryneform bacteria, a L-lysine-producing strain in which the polynucleotide is inactivated, and a method for producing L-lysine using the same.
    Type: Application
    Filed: October 14, 2014
    Publication date: April 16, 2015
    Inventors: Un Hwan HA, Yong Jae KIM, Jung Hoon LEE, Hee Sung SHIN, Jun Ok MOON, Hyung Joon KIM, Kwang Ho LEE
  • Publication number: 20150104851
    Abstract: The present invention relates to novel mutants with cyclase activity and use thereof in a method for biocatalytic cyclization of terpenes, such as in particular for the production of isopulegol by cyclization of citronellal; a method for the preparation of menthol and methods for the biocatalytic conversion of further compounds with structural motifs similar to terpene.
    Type: Application
    Filed: December 4, 2014
    Publication date: April 16, 2015
    Inventors: Michael Breuer, Bernhard Hauer, Dieter Jendrossek, Gabrielle Siedenburg, Juergen Pleiss, Demet Sirim, Silvia Racolta
  • Publication number: 20150104838
    Abstract: The present invention relates to a recombinant microorganism having enhanced ability of producing putrescine at high yield, which is generated by weakening the activity of NCgl1469 in a microorganism of Corynebacterium genus that is modified to produce putrescine, and a method for producing putrescine using the same.
    Type: Application
    Filed: January 11, 2013
    Publication date: April 16, 2015
    Inventors: Su Jin Choi, Hyang Choi, Min Sun Kang, Sung Hoo Jhon, Kyoung Min Lee, Hye Won Um, Young Lyeol Yang
  • Publication number: 20150093798
    Abstract: A microorganism capable of producing 1,4-butanediol and a method of producing 1,4-butanediol using the same.
    Type: Application
    Filed: September 29, 2014
    Publication date: April 2, 2015
    Inventors: Yukyung Jung, Kwangmyung Cho, Jinhwan Park, Hwayoung Cho
  • Publication number: 20150093781
    Abstract: The invention relates to an isolated, genetically modified, living non-mammal organism, having increased HMG-CoA-reductase activity compared to the wild type, and having reduced C24-methyltransferase and/or delta22-desaturase activity compared to the wild type. The invention is characterized in that the organism has increased dehydrocholesterol-delta70-reductase activity compared to the wild type. The invention further relates to different uses of such an organism, to a test kit comprising such an organism, and to a membrane extract of such an organism.
    Type: Application
    Filed: August 13, 2014
    Publication date: April 2, 2015
    Inventors: Michael Schilling, Christine Lang, Andreas Raab
  • Patent number: 8993743
    Abstract: The present invention relates to a nucleic acid molecule encoding a chimeric protein having the biochemical activity of a surface active protein, wherein said chimeric protein comprises: (a) an N-terminal portion of a first surface active protein, wherein the N-terminal portion is devoid of between 0 and 10 of the most N-terminal amino acids of the mature first surface active protein; and, C-terminally thereof, (b) a C-terminal portion of a second surface active protein, wherein the C-terminal portion is devoid of between 0 and 10 of the most C-terminal amino acids of the mature second surface active protein. The present invention further relates to a vector, a non-human host and a method for the production of a chimeric protein having the biochemical activity of a surface active protein. In addition, the present invention relates to a chimeric protein encoded by the nucleic acid molecule of the invention and a composition comprising the chimeric protein.
    Type: Grant
    Filed: February 18, 2011
    Date of Patent: March 31, 2015
    Assignee: B.R.A.I.N. Biotechnology Research and Information Network AG
    Inventors: Guido Meurer, Esther Gabor, Anke Bachert, Jürgen Eck
  • Publication number: 20150087028
    Abstract: The invention relates to recombinant expression of variant forms of M. thermophila CBH1a and homologs thereof, having improved thermoactivity, specific activity, and other desirable properties. Also provided are methods for producing ethanol and other valuable organic compounds by combining cellobiohydrolase variants with cellulosic materials.
    Type: Application
    Filed: March 12, 2013
    Publication date: March 26, 2015
    Inventors: Behnaz Behrouzian, Xinkai Xie, Kui Chan, Xiyun Zhang, Vesna Mitchell, Douglas A. Hattendorf
  • Publication number: 20150079641
    Abstract: The invention relates to an isolated nucleotide sequence encoding an amino acid sequence that is at least ?90%, ?92%, ?94%, ?96%, ?97%, ?98%, ?99% or 100%, preferably ?97%, particularly preferably ?98%, very particularly preferably ?99%, and extremely preferably 0%, identical to the amino acid sequence of SEQ ID NO:2, wherein SEQ ID NO:2, at position 553, or at a corresponding position of the amino acid sequence, has a proteinogenic amino acid other than L-tyrosine, to a microorganism comprising the nucleotide sequence and also to a process for producing fine chemicals using this microorganism.
    Type: Application
    Filed: April 12, 2013
    Publication date: March 19, 2015
    Applicant: EVONIK INDUSTRIES AG
    Inventors: Robert Gerstmeir, Iris Wiegrabe
  • Publication number: 20150079644
    Abstract: The present invention provides a method for conveniently and efficiently producing a 3-acetylamino-4-hydroxybenzoic acid-type compound that is a stable compound by a process using a microorganism. Specifically the present invention provides a microorganism having an ability to produce 3-amino-4-hydroxybenzoic acid, that is modified so as to increase an activity to form 3-amino-4-hydroxybenzoic acid from dihydroxyacetone phosphate and aspartate semialdehyde, wherein the microorganism is modified so as to increase an N-hydroxyarylamine O-acetyltransferase (NhoA) activity, as well as a method for producing the 3-acetylamino-4-hydroxybenzoic acid-type compound using such a microorganism.
    Type: Application
    Filed: November 19, 2014
    Publication date: March 19, 2015
    Applicant: AJINOMOTO CO., INC.
    Inventors: Fumi Shozui, Yoshinori Tajima, Keiichi Yokoyama
  • Publication number: 20150072399
    Abstract: The present invention relates to methods, systems and compositions, including genetically modified microorganisms, directed to achieve decreased microbial conversion of 3-hydroxypropionic acid (3-HP) to aldehydes of 3-HP. In various embodiments this is achieved by disruption of particular aldehyde dehydrogenase genes, including multiple gene deletions. Among the specific nucleic acids that are deleted whereby the desired decreased conversion is achieved are aldA, aldB, puuC), and usg of E. coli. Genetically modified microorganisms so modified are adapted to produce 3-HP, such as by approaches described herein.
    Type: Application
    Filed: May 12, 2014
    Publication date: March 12, 2015
    Applicant: OPX Biotechnologies, Inc.
    Inventors: Michael D. Lynch, Christopher P. Mercogliano, Matthew L. Lipscomb, Tanya E. W. Lipscomb
  • Publication number: 20150064751
    Abstract: The embodiments described herein pertain to cells, and methods for preparing cells, that can be used as biocatalysts by altering enzymes that compete for a substrate or product of a pathway of interest such that the targeted enzyme is sensitive to a site-specific protease, which protease is expressed but relocated in the cell to a site where it is not in contact with the targeted enzyme in the intact cell. Upon cell lysis, the protease contacts the target enzyme, which is then inactivated by protease cleavage.
    Type: Application
    Filed: November 14, 2014
    Publication date: March 5, 2015
    Applicants: The Board of Trustees of the Leland Stanford Junior University, GreenLight Biosciences, Inc.
    Inventor: James R. Swartz
  • Publication number: 20150064758
    Abstract: A recombinant microorganism including pyruvate dehydrogenase having increased activity may increase 1,4-BDO production under anaerobic conditions, as well as a method for preparing same, and method of using same to produce a C4 chemical.
    Type: Application
    Filed: August 29, 2014
    Publication date: March 5, 2015
    Inventors: Wooyong Lee, Joonsong Park, Youngmin Lee, Jaechan Park, Jinhwan Park
  • Publication number: 20150064743
    Abstract: The invention relates to methods for producing mogrosides with the aid of enzymes. In particular the invention proposes various biosynthetic pathways useful for mogroside production and enzymes useful for mogroside production are provided. Furthermore, the invention provides recombinant hosts useful in performing the methods of the invention.
    Type: Application
    Filed: October 1, 2014
    Publication date: March 5, 2015
    Inventors: Yaoquan Liu, Jorgen Hansen, Jens Houghton-Larsen, Muthuswamy Panchapagesa Murali, Sathish Kumar, Nina Nicoline Rasmussen
  • Publication number: 20150064753
    Abstract: A Corynebacterium including an NAD+ dependent formate dehydrogenase gene, and a method of producing C4 dicarboxylic acid using the Corynebacterium.
    Type: Application
    Filed: September 5, 2014
    Publication date: March 5, 2015
    Inventors: Soonchun CHUNG, Joonsong PARK, Jinhwan PARK, Jiae YUN, Jaechan PARK, Kwangmyung CHO
  • Publication number: 20150056669
    Abstract: The present invention provides various combinations of genetic modifications to a transformed host cell that provide increase conversion of carbon to a chemical product. The present invention also provides methods of fermentation and methods of making various chemical products.
    Type: Application
    Filed: March 17, 2014
    Publication date: February 26, 2015
    Inventors: Hans LIAO, Christopher Patrick MERCOGLIANO, Travis Robert WOLTER, Michael Tai Man LOUIE, Wendy Kathleen RIBBLE, Tanya LIPSCOMB, Eileen Colie SPINDLER, Michael D LYNCH
  • Publication number: 20150057461
    Abstract: The present invention relates to a transformant which is transformed to express Baeyer-Villiger monooxygenase (BVMO), a method for producing C5-C14 medium-chain ?-hydroxy fatty acids, ?,?-dicarboxylic acids, ?-amino fatty acids, or alcohols from C16-C20 long-chain fatty acids by biotransformation using the transformant, a method for producing a fatty acid derivative having an ester group which is introduced into the chain thereof from keto fatty acid using the BVMO, and novel ?-hydroxy fatty acids which are prepared by the method. Degradation products such as C5 to C14 ?-hydroxy fatty acids, ?,?-dicarboxylic acids, ?-amino fatty acids, alcohols can be produced in a large amount from C16 to C20 long-chain fatty acids contained in a medium by biotransformation using a transformant capable of expressing BVMO of the present invention. Therefore, it can be widely used to produce ?-hydroxy fatty acids, ?,?-dicarboxylic acids, ?-amino fatty acids or alcohols in a more safe and economic manner.
    Type: Application
    Filed: April 5, 2013
    Publication date: February 26, 2015
    Inventors: Jin Byung Park, Ji Won Song, Eun Yeong Jeon
  • Publication number: 20150056667
    Abstract: The present invention relates to a hydantoinase having an amino acid sequence selected from (i) or (ii), with (i) amino acid sequence selected from SEQ ID NO: 6-20 and SEQ ID NO: 73-119 (ii) amino acid sequence wherein in the amino acid sequence of SEQ ID NO: 6-20 and SEQ ID NO: 73-119, 1 to 75 amino acid residues have been substituted, deleted, inserted and/or added, and wherein further the catalytic activity of the hydantoinase is higher by a factor of at least 1.2 than the catalytic activity of the hydantoinase having amino acid sequence SEQ ID NO: 1, The present invention further relates to a process for preparing amino acids, wherein said hydantoinase is used.
    Type: Application
    Filed: November 16, 2012
    Publication date: February 26, 2015
    Inventors: Steffen Osswald, Heiko Schuster, Jürgen Roos, Andreas Karau, Ulrich Schwaneberg, Ronny Martinez, Hemanshu Mundhada, Ursula Holter
  • Publication number: 20150056666
    Abstract: The invention relates to an isolated polynucleotide having promoter activity, a variant of the promoter of the gap gene coding for glyceraldehyde-3-phosphate dehydrogenase; and to a microorganism which produces and/or secretes a fine chemical, the microorganism including the isolated polynucleotide having promoter activity, which enables various genes to be overexpressed in comparison with the particular starting strain; and to a process for preparing fine chemicals using the microorganism.
    Type: Application
    Filed: November 4, 2014
    Publication date: February 26, 2015
    Applicant: Evonik Degussa GmbH
    Inventors: Alexander RETH, Brigitte Bathe, Stephan Hans, Wilfried Claes
  • Publication number: 20150056670
    Abstract: A genetically modified microorganism comprising a polynucleotide encoding ?-ketoglutarate synthase or a mutant thereof, and a polynucleotide encoding pyruvate carboxylase or a mutant thereof; wherein the genetically modified microorganism has decreased malate quinone oxidoreductase activity and/or decreased phosphoenolpyruvate carboxykinase activity compared to an unmodified microorganism of the same type, and wherein the genetically modified microorganism produces 4-hydroxybutyrate.
    Type: Application
    Filed: August 25, 2014
    Publication date: February 26, 2015
    Inventors: Youngmin LEE, Wooyong Lee, Jinwoo Kim, Jaechan Park, Jinhwan Park, Hwayoung Cho
  • Publication number: 20150050708
    Abstract: Provided herein are non-naturally occurring microbial organisms having a formaldehyde fixation pathway and a formate assimilation pathway, which can further include a methanol metabolic pathway, a methanol oxidation pathway, a hydrogenase and/or a carbon monoxide dehydrogenase. These microbial organisms can further include a butadiene, 1,3-butanediol, crotyl alcohol or 3-buten-2-ol pathway. Additionally provided are methods of using such microbial organisms to produce butadiene, 1,3-butanediol, crotyl alcohol or 3-buten-2-ol.
    Type: Application
    Filed: March 14, 2014
    Publication date: February 19, 2015
    Applicant: Genomatica, Inc.
    Inventors: Anthony P. BURGARD, Robin E. OSTERHOUT, Priti PHARKYA, Stefan ANDRAE
  • Publication number: 20150044745
    Abstract: A recombinant Corynebacterium genus microorganism, and a method of producing C4 dicarboxylic acid under anaerobic conditions using the Corynebacterium genus microorganism.
    Type: Application
    Filed: August 11, 2014
    Publication date: February 12, 2015
    Inventors: Soonchun CHUNG, Joonsong Park, Jinhwan Park, Jiae Yun, Jaechan Park, Kwangmyung Cho
  • Patent number: 8951937
    Abstract: A group of bacterial dihydroxy-acid dehydratases having a [2Fe-2S] cluster was discovered. Bacterial [2Fe-2S] DHADs were expressed as heterologous proteins in bacteria and yeast cells, providing DHAD activity for conversion of 2,3-dihydroxyisovalerate to ?-ketoisovalerate or 2,3-dihydroxymethylvalerate to ?-ketomethylvalerate. Isobutanol and other compounds may be synthesized in pathways that include bacterial [2Fe-2S] DHAD activity.
    Type: Grant
    Filed: March 15, 2013
    Date of Patent: February 10, 2015
    Assignee: Butamax Advanced Biofuels LLC
    Inventors: Dennis Flint, Steven Cary Rothman, Wonchul Suh, Jean-Francois Tomb, Rick W. Ye
  • Publication number: 20150037850
    Abstract: The present invention relates to a quinolinic acid-producing recombinant microorganism expressing a fusion protein of L-aspartate oxidase and quinolinate synthase linked via a linker, and a method for producing quinolinic acid using the same.
    Type: Application
    Filed: January 3, 2013
    Publication date: February 5, 2015
    Inventors: Yong Uk Shin, So Young Kim, In Kyung Heo, Ju Eun Kim, Sung Kwang Son, Jae Hee Lee, Ji Hyun Lee, Chang Gyeom Kim
  • Publication number: 20150037860
    Abstract: This document describes biochemical pathways for producing isoprene by forming two vinyl groups in a central precursor produced from isobutyryl-CoA, 3-methyl-2-oxopentanoate, or 4-methyl-2-oxopentanoate as well as recombinant hosts for producing isoprene.
    Type: Application
    Filed: August 5, 2014
    Publication date: February 5, 2015
    Inventors: Adriana Leonora Botes, Alex Van Eck Conradie
  • Publication number: 20150031101
    Abstract: A genetically engineered bacterial cell wherein activity of a pathway in the cell of converting ?-ketoglutarate into succinate semialdehyde; or activity of succinyl semialdehyde dehydrogenase in the cell is increased compared to the activity in a non-genetically engineered cell of the same type, and a method of producing succinic acid by using the same.
    Type: Application
    Filed: July 28, 2014
    Publication date: January 29, 2015
    Inventors: Joonsong PARK, Jinhwan Park, Soonchun Chung, Jiae Yun, Kwangmyung Cho
  • Publication number: 20150024009
    Abstract: The invention provides an isolated genetically modified non-mammalian organism, wherein the activity of acyl-CoA:sterol acyltransferase/sterol O-acyltransferase (EC 2.3.1.26) and/or diacylglycerol acyltransferase/diacylglycerol O-acyltranferase (EC 2.3.1.20) and/or lecithin cholesterol acyl transferase/phospholipid: diacylglycerol acyltransferase (EC 2.3.1.158) and/or acyl CoA-wax alcohol acyltransferase (EC 2.3.1.75) is reduced or abolished in comparison with a corresponding wildtype organism, methods of use of such an organism, shuttle vehicles for making such an organism and methods for producing such an organism.
    Type: Application
    Filed: September 19, 2014
    Publication date: January 22, 2015
    Applicant: ORGANOBALANCE GMBH
    Inventors: Christine Lang, Andreas Raab
  • Publication number: 20150011482
    Abstract: Nutritive proteins are provided. In some embodiments the nutritive proteins comprise a first polypeptide sequence comprising a fragment of a naturally-occurring nutritive protein. In some embodiments the fragment comprises at least one of a) an enhanced ratio of branch chain amino acid residues to total amino acid residues present in the nutritive protein; b) an enhanced ratio of leucine residues to total amino acid residues present in the nutritive protein; and c) an enhanced ratio of essential amino acid residues to total amino acid residues present in the nutritive protein.
    Type: Application
    Filed: March 15, 2013
    Publication date: January 8, 2015
    Inventors: David Arthur Berry, Brett Adam Boghigian, Nathaniel W. Silver, Geoffrey von Maltzahn, Rajeev Chillakuru, Michael Hamill
  • Publication number: 20150010969
    Abstract: The disclosure provides a method for creating a transformant having significantly improved glucaric acid-producing capability and a method for efficiently producing glucaric acid using the transformant.
    Type: Application
    Filed: February 19, 2013
    Publication date: January 8, 2015
    Applicant: ASAHI KASEI CHEMICALS CORPORATION
    Inventors: Kazunobu Konishi, Shinichi Imazu
  • Publication number: 20150004661
    Abstract: A microorganism expressing a vector encoding a CoA-dependent succinate semialdehyde dehydrogenase to efficiently produce a C4 compound, and methods for the use thereof.
    Type: Application
    Filed: June 30, 2014
    Publication date: January 1, 2015
    Inventors: Hong-soon RHEE, Young-min Lee, Jin-woo Kim, Eun-Young Park, Yeo-ju Song, Jin-hwan Park, Kwang-myung Cho
  • Patent number: 8921083
    Abstract: A modified microorganism for producing a high yield of 1,4-butanediol, and a method of producing 1,4-butanediol using the modified microorganism, wherein the modified microorganism is altered so as to delete or disrupt expression of at least one of transcription regulatory factor NCg12886, GCN5-related N-acetyltransferase NCg12090, hypothetical protein NCg10224 or sugar phosphate isomerase/epimerase NCg12956.
    Type: Grant
    Filed: July 19, 2013
    Date of Patent: December 30, 2014
    Assignee: Samsung Electronics Co., Ltd.
    Inventors: Woo Yong Lee, Hyun Min Koo, Sung Soo Kim, Jae Young Kim, Jae Chan Park
  • Publication number: 20140377816
    Abstract: The present invention relates to microorganisms of corynebacterium which can utilize xylose and to a method for producing L-lysine using same. More particularly, the present invention relates to microorganisms of corynebacterium which are modified, in which genes encoding xylose isomerase and xylulokinase which are xylose synthases are introduced to express the xylose synthase. The present invention also relates to a method for producing L-lysine, comprising a step of culturing the modified microorganisms of corynebacterium using xylose as a carbon source, and recovering L-lysine from the culture.
    Type: Application
    Filed: January 10, 2013
    Publication date: December 25, 2014
    Inventors: So Yeon Rah, Lan Huh, Chang Gyeom Kim, Kwang Ho Lee, Jun Ok Moon, Kyung Han Lee, Jin Seok Sung, Hyung Joon Kim
  • Publication number: 20140371417
    Abstract: The invention provides non-naturally occurring microbial organisms having a 4-hydroxybutyrate, gamma-butyrolactone, 1,4-butanediol, 4-hydroxybutanal, 4-hydroxybutyryl-CoA and/or putrescine pathway and being capable of producing 4-hydroxybutyrate, wherein the microbial organism comprises one or more genetic modifications. The invention additionally provides methods of producing 4-hydroxybutyrate, gamma-butyrolactone, 1,4-butanediol, 4-hydroxybutanal, 4-hydroxybutyryl-CoA and/or putrescine or related products using the microbial organisms.
    Type: Application
    Filed: April 25, 2014
    Publication date: December 18, 2014
    Applicant: Genomatica, Inc.
    Inventors: Priti PHARKYA, Anthony P. BURGARD, Stephen J. VAN DIEN, Robin E. OSTERHOUT, Mark J. BURK, John D. TRAWICK, Michael P. KUCKINSKAS, Brian STEER
  • Publication number: 20140370549
    Abstract: The disclosure provides a method of producing a scyllo-inositol or a new scyllo-inositol derivative in a one-step process, from ubiquitous and inexpensive raw materials. Also provided is a scyllo-inositol derivative bonded to saccharides such as glucose and similar.
    Type: Application
    Filed: January 22, 2013
    Publication date: December 18, 2014
    Applicant: ASAHI KASEI CHEMICALS CORPORATION
    Inventors: Kazunobu Konishi, Shinichi Imazu
  • Patent number: 8911978
    Abstract: The present invention concerns a modified microorganism for the biological preparation of an hydroxy acid of formula (I) wherein the microorganism comprises a two-step metabolic pathway for the production of the said hydroxy acid from a hydroxy keto-acid of formula (II) through an intermediate hydroxy-aldehyde of formula (III), wherein EA1 is an enzyme having a 2-keto-acid decarboxylase activity, and EA2 is an enzyme having hydroxy aldehyde dehydrogenase activity. The invention also concerns a method for the fermentative production of a hydroxy acid.
    Type: Grant
    Filed: June 28, 2011
    Date of Patent: December 16, 2014
    Assignee: Metabolic Explorer
    Inventors: Philippe Soucaille, Cedric Boisart
  • Publication number: 20140363859
    Abstract: The present invention relates to a recombinant microorganism having enhanced ability to produce putrescine at high yield, wherein the activity of NCgl0101 is weakened in a microorganism of genus Corynebacterium that has been modified to produce putrescine, and a method for producing putrescine using the same.
    Type: Application
    Filed: January 21, 2013
    Publication date: December 11, 2014
    Inventors: Kyoung Min Lee, Seon Hye Kim, Hye Won Um, Min Sun Kang, Su Jin Choi, Hee Kyoung Jung, Sung Hoo Jhon, Hongxian Li, Won Sik Gwak, Chong Ho Lee, Young Lyeol Yang
  • Publication number: 20140363864
    Abstract: A non-naturally occurring microbial organism includes a microbial organism having a 1,4-cyclohexanedimethanol pathway that includes at least one exogenous nucleic acid encoding a 1,4-cyclohexanedimethanol pathway enzyme expressed in a sufficient amount to produce 1,4-cyclohexanedimethanol. A method for producing 1,4-cyclohexanedimethanol includes culturing a non-naturally occurring microbial organism having a 1,4-cyclohexanedimethanol pathway. The pathway includes at least one exogenous nucleic acid encoding a 1,4-cyclohexanedimethanol pathway enzyme expressed in a sufficient amount to produce 1,4-cyclohexanedimethanol, under conditions and for a sufficient period of time to produce 1,4-cyclohexanedimethanol.
    Type: Application
    Filed: January 10, 2014
    Publication date: December 11, 2014
    Applicant: Genomatica, Inc.
    Inventors: Priti Pharkya, Mark J. Burk
  • Publication number: 20140363858
    Abstract: Provided herein are methods for producing an ortho-hydroxylated phenylpropanoid. In one embodiment the method includes culturing a microbe that includes HpaBC activity in the presence of a phenylpropanoid substrate. Also provided are genetically engineered microbes engineered to have greater levels of HpaB and/or HpaC than a control microbe.
    Type: Application
    Filed: June 10, 2014
    Publication date: December 11, 2014
    Inventors: YAJUN YAN, YUHENG LIN
  • Publication number: 20140363847
    Abstract: An acetyl-CoA-producing microorganism, which is capable of efficiently synthesizing acetyl-CoA using carbon dioxide, and a substance production method using the same are provided. An acetyl-CoA-producing microorganism including an acetyl-CoA production cycle obtained by imparting at least one type of enzymatic activity selected from the group consisting of malate thiokinase, malyl-CoA lyase, glyoxylate carboligase, 2-hydroxy-3-oxopropionate reductase, and hydroxypyruvate reductase, to a microorganism.
    Type: Application
    Filed: July 27, 2012
    Publication date: December 11, 2014
    Inventors: Ryota Fujii, Tomokazu Shirai, Tadashi Araki, Koh Amano, Yoshiko Matsumoto, Toshihiro Tateno, Nozomi Takebayashi, Takashi Morishige, Hitoshi Takahashi, Mitsufumi Wada, Hiroshi Shimizu, Chikara Furusawa, Takashi Hirasawa, Tomonori Hidesaki, Ayako Endo, Dominik Lukas Jürgen-Lohmann, Anjali Madhavan
  • Publication number: 20140356919
    Abstract: A non-naturally occurring microbial organism has cyclohexanone pathways that include at least one exogenous nucleic acid encoding a cyclohexanone pathway enzyme. A pathway includes a 2-ketocyclohexane-1-carboxyl-CoA hydrolase (acting on C—C bond), a 2-ketocyclohexane-1-carboxylate decarboxylase and an enzyme selected from a 2-ketocyclohexane-1-carboxyl-CoA hydrolase (acting on thioester), a 2-ketocyclohexane-1-carboxyl-CoA transferase, and a 2-ketocyclohexane-1-carboxyl-CoA synthetase.
    Type: Application
    Filed: February 4, 2014
    Publication date: December 4, 2014
    Inventors: Robin E. Osterhout, Anthony P. Burgard, Mark J. Burk, Priti Pharkya
  • Publication number: 20140356518
    Abstract: The present invention relates to a method for producing highly-concentrated L-amino acid and riboflavin simultaneously, and a microorganism for simultaneously producing L-amino acid and riboflavin. Specifically, the present invention relates to a modified microorganism for producing L-lysine or L-threonine, and riboflavin simultaneously, wherein the microorganism belonging to Corynebacterium sp. capable of producing L-lysine or L-threonine is modified by enhancing the activity of an enzyme family expressed by a rib operon which contains riboflavin biosynthesis gene family. Also, the present invention relates to a method for the simultaneous production of L-lysine or L-threonine, and riboflavin using the modified microorganism, and relates to a formulation or granular formulation, feed, and feed additive, containing L-lysine or L-threonine, and riboflavin produced from a culture medium of the modified microorganism.
    Type: Application
    Filed: October 17, 2012
    Publication date: December 4, 2014
    Inventors: Sang Hee Park, Jun Ok Moon, Sang Jo Lim, Do Hyun Kwon, Kyung Han Lee, Jin Suck Sung, Hyun Joon Kim
  • Publication number: 20140356916
    Abstract: Recombinant microorganisms with deregulated succinyl-CoA synthetase activity, as well as the uses for producing lysine, ?-lysine, cadaverine or N-acetylcadaverine thereby are provided. Recombinant polypeptides comprising an amino acid sequence being at least 80% identical to SEQ ID NO: 51 or 53 are also provided. The method of producing fine chemicals using said recombinant microorganisms, in particular the method of producing lysine, or derivatives thereof, such as ?-lysine, cadaverine or N-acetylcadaverine is further provided.
    Type: Application
    Filed: December 14, 2012
    Publication date: December 4, 2014
    Applicant: BASF SE
    Inventors: Christoph Wittmann, Stefanie Kind, Judith Becker
  • Publication number: 20140356929
    Abstract: The aim of the invention is to improve the secretion of a protein from a host cell in order to increase the product yield of protein in a fermentation process. This is achieved by an expression vector comprising a) a promoter sequence and b) a nucleic acid sequence that codes for a protein. The protein comprises a signal peptide and an additional amino acid sequence, and the signal peptide comprises an amino acid sequence that is at least 80% identical to the amino acid sequence specified in SEQ ID NO 2, at least 80% identical to the amino acid sequence specified in SEQ ID NO 4, at least 80% identical to the amino acid sequence specified in SEQ ID NO 6, or the signal peptide comprises an amino acid sequence that is structurally homologous to at least one of said sequences.
    Type: Application
    Filed: May 25, 2012
    Publication date: December 4, 2014
    Applicant: BASF SE
    Inventors: Christian Degering, Thorsten Eggert, Stefan Evers, Karl-Heinz Maurer, Johannes Bongaerts
  • Publication number: 20140349349
    Abstract: The present invention is related to recombinant host cells comprising: (i) at least one deletion, mutation, and/or substitution in an endogenous gene encoding a polypeptide that converts pyruvate to acetaldehyde, acetyl-phosphate, or acetyl-CoA; and (ii) a heterologous polynucleotide encoding a polypeptide having phosphoketolase activity. The present invention is also related to recombinant host cells further comprising (iii) a heterologous polynucleotide encoding a polypeptide having phosphotransacetylase activity.
    Type: Application
    Filed: July 18, 2014
    Publication date: November 27, 2014
    Inventors: MICHAEL DAUNER, Lori Ann Maggio-Hall, Jean-Francois Tomb
  • Patent number: 8895276
    Abstract: The present invention relates to a microorganism variant having the ability to produce hydrocarbons, including alkane, and a method of producing hydrocarbons, including alkane, using the same, and more particularly, to a microorganism variant obtained by introducing a gene encoding an enzyme converting fatty acyl-acp to free fatty acid, a gene encoding an enzyme converting free fatty acid to fatty acyl-CoA, a gene encoding an enzyme converting fatty acyl-CoA to fatty aldehyde and a gene encoding an enzyme converting fatty aldehyde to alkane into a microorganism improved so as to be suitable for the production of hydrocarbons, including alkane, and a method of producing hydrocarbons, including alkane, using the same. The microorganism variant of the present invention has high potential to be used to improve strains by additional metabolic flux engineering, and thus is useful for the industrial production of hydrocarbons, including alkane.
    Type: Grant
    Filed: June 10, 2011
    Date of Patent: November 25, 2014
    Assignee: Korea Advanced Institute of Science and Technology
    Inventors: Sang Yup Lee, Yong Jun Choi
  • Patent number: 8883460
    Abstract: The present invention relates to a polynucleotide that is active to an acetyl glutamate synthase and acetyl ornithinase which are associated with ornithine or arginine biosynthesis from Corynebacterium glutamicum. The present invention also relates to a polypeptide encoded by said polynucleotide, a recombinant vector comprising said polynucleotide, to a transformant obtained by introducing said recombinant vector to a host microorganism for producing L-ornithine or L-arginine, and transforming the recombinant vector, and to a method for producing L-ornithine or L-arginine by culturing said transformant. The activity of the transformant of the present invention to an acetyl glutamate synthase and acetyl ornithinase is increased as compared to an intrinsic activity, and thus L-ornithine or L-arginine can be produced, at a high yield rate, from the transformant of the present invention.
    Type: Grant
    Filed: December 29, 2010
    Date of Patent: November 11, 2014
    Assignee: CJ Cheiljedang Corp.
    Inventors: Jin-Man Cho, Hye-Won Kim, Ji-Hye Lee, Jae-Yong Cho