Brevibacterium Or Corynebacterium Patents (Class 435/252.32)
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Publication number: 20140349349Abstract: The present invention is related to recombinant host cells comprising: (i) at least one deletion, mutation, and/or substitution in an endogenous gene encoding a polypeptide that converts pyruvate to acetaldehyde, acetyl-phosphate, or acetyl-CoA; and (ii) a heterologous polynucleotide encoding a polypeptide having phosphoketolase activity. The present invention is also related to recombinant host cells further comprising (iii) a heterologous polynucleotide encoding a polypeptide having phosphotransacetylase activity.Type: ApplicationFiled: July 18, 2014Publication date: November 27, 2014Inventors: MICHAEL DAUNER, Lori Ann Maggio-Hall, Jean-Francois Tomb
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Patent number: 8895276Abstract: The present invention relates to a microorganism variant having the ability to produce hydrocarbons, including alkane, and a method of producing hydrocarbons, including alkane, using the same, and more particularly, to a microorganism variant obtained by introducing a gene encoding an enzyme converting fatty acyl-acp to free fatty acid, a gene encoding an enzyme converting free fatty acid to fatty acyl-CoA, a gene encoding an enzyme converting fatty acyl-CoA to fatty aldehyde and a gene encoding an enzyme converting fatty aldehyde to alkane into a microorganism improved so as to be suitable for the production of hydrocarbons, including alkane, and a method of producing hydrocarbons, including alkane, using the same. The microorganism variant of the present invention has high potential to be used to improve strains by additional metabolic flux engineering, and thus is useful for the industrial production of hydrocarbons, including alkane.Type: GrantFiled: June 10, 2011Date of Patent: November 25, 2014Assignee: Korea Advanced Institute of Science and TechnologyInventors: Sang Yup Lee, Yong Jun Choi
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Patent number: 8883460Abstract: The present invention relates to a polynucleotide that is active to an acetyl glutamate synthase and acetyl ornithinase which are associated with ornithine or arginine biosynthesis from Corynebacterium glutamicum. The present invention also relates to a polypeptide encoded by said polynucleotide, a recombinant vector comprising said polynucleotide, to a transformant obtained by introducing said recombinant vector to a host microorganism for producing L-ornithine or L-arginine, and transforming the recombinant vector, and to a method for producing L-ornithine or L-arginine by culturing said transformant. The activity of the transformant of the present invention to an acetyl glutamate synthase and acetyl ornithinase is increased as compared to an intrinsic activity, and thus L-ornithine or L-arginine can be produced, at a high yield rate, from the transformant of the present invention.Type: GrantFiled: December 29, 2010Date of Patent: November 11, 2014Assignee: CJ Cheiljedang Corp.Inventors: Jin-Man Cho, Hye-Won Kim, Ji-Hye Lee, Jae-Yong Cho
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Publication number: 20140328801Abstract: A non-therapeutic method of accumulating a polymeric or high molecular weight molecular product within a bacterial microcompartment in bacterial cytoplasm, which method employs a recombinant bacteria which is transformed to express a microcompartment containing an enzyme capable of converting a low molecular weight substrate into a polymeric or high molecular weight product, the method comprising the steps of: incubating the recombinant bacteria with the low-molecular weight substrate, or a precursor of the low molecular weight substrate which is capable of being metabolised to the substrate within the recombinant bacteria, such that the substrate or precursor is taken up by the bacteria, wherein the substrate enters the microcompartment and the enzyme within the microcompartment converts the substrate to a polymeric or high molecular weight molecular product, and wherein the polymeric or high molecular weight molecular product is accumulated within the microcompartment due to its size.Type: ApplicationFiled: September 27, 2012Publication date: November 6, 2014Inventors: Michael Prentice, Martin Warren, Mingzhi Liang
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Patent number: 8871478Abstract: A Corynebacterium glutamicum transformant having the capability of producing isobutanol and the following genes (1) to (5): (1) a gene which encodes an enzyme having acetohydroxy acid synthase activity; (2) a gene which encodes an enzyme having acetohydroxy acid isomeroreductase activity; (3) a gene which encodes an enzyme having dihydroxy acid dehydratase activity; (4) a gene which encodes an enzyme having 2-keto acid decarboxylase activity; and (5) a gene which encodes an enzyme having alcohol dehydrogenase activity, at least one of the genes being endogenous, and at least one of the genes being exogenous, efficiently produces isobutanol.Type: GrantFiled: March 29, 2010Date of Patent: October 28, 2014Assignee: Research Institute of Innovative Technology for the EarthInventors: Hideaki Yukawa, Masayuki Inui
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Patent number: 8871489Abstract: This invention relates to the metabolic evolution of a microbial organism previously optimized for producing an organic acid in commercially significant quantities under fermentative conditions using a hexose sugar as sole source of carbon in a minimal mineral medium. As a result of this metabolic evolution, the microbial organism acquires the ability to use pentose sugars derived from cellulosic materials for its growth while retaining the original growth kinetics, the rate of organic acid production and the ability to use hexose sugars as a source of carbon. This invention also discloses the genetic change in the microorganism that confers the ability to use both the hexose and pentose sugars simultaneously in the production of commercially significant quantities of organic acids.Type: GrantFiled: November 17, 2010Date of Patent: October 28, 2014Assignee: Myriant CorporationInventors: Tammy Grabar, Wei Gong, R. Rogers Yocum
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Patent number: 8871488Abstract: The present invention is related to recombinant host cells comprising: (i) at least one deletion, mutation, and/or substitution in an endogenous gene encoding a polypeptide that converts pyruvate to acetaldehyde, acetyl-phosphate or acetyl-CoA; and (ii) a heterologous polynucleotide encoding a polypeptide having phosphoketolase activity. The present invention is also related to recombinant host cells further comprising (iii) a heterologous polynucleotide encoding a polypeptide having phosphotransacetylase activity.Type: GrantFiled: June 15, 2011Date of Patent: October 28, 2014Assignee: Butamax Advanced Biofuels LLCInventors: Michael Dauner, Lori Ann Maggio-Hall, Jean-Francois Tomb
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Publication number: 20140315310Abstract: The invention provides, inter alia, recombinase-based systems that provide for integrated logic and memory in living cells.Type: ApplicationFiled: December 13, 2013Publication date: October 23, 2014Applicant: Massachusetts Institute of TechnologyInventors: Timothy Kuan-Ta Lu, Piro Siuti
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Publication number: 20140308717Abstract: The present invention relates to a method comprising the steps a) providing a secondary alcohol, b) oxidizing the secondary alcohol by contacting it with an NAD(P)+-dependent alcohol dehydrogenase and c) contacting the oxidation product of step a) with a transaminase, wherein the NAD(P)+-dependent alcohol dehydrogenase and/or the transaminase is a recombinant or isolated enzyme, to a whole cell catalyst for carrying out the method, and to the use of such a whole cell catalyst for oxidizing a secondary alcohol.Type: ApplicationFiled: July 27, 2012Publication date: October 16, 2014Applicant: Evonik Degussa GmbHInventors: Thomas Haas, Markus Poetter, Jan Christoph Pfeffer, Wolfgang Kroutil, Arne Skerra, Alexandra Lerchner, Katharina Christin Tauber, Johann H. Sattler, Steffen Schaffer
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Patent number: 8859236Abstract: The present invention relates to a microorganism with improved production of 5?-xanthosine monophosphate and 5?-guanine monophosphate, and more specifically, to a Corynebacterium sp. microorganism having increased proline dehydrogenase activity compared with an intrinsic activity thereof, a method for producing 5?-xanthosine monophosphate or 5?-guanine monophosphate from the culture medium obtained by culturing the transformed microorganism, and a use of the microorganism for production of 5?-xanthosine monophosphate or 5?-guanine monophosphate.Type: GrantFiled: March 17, 2011Date of Patent: October 14, 2014Assignee: CJ Cheiljedang CorporationInventors: Jin Man Cho, Jin Nam Lee, Hye Won Kim, Ji Hye Lee, Nan Young Yoon, Kwang Woo Lee, Yoon Seok Oh, Jang Hee Park
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Patent number: 8852903Abstract: Recombinant microorganisms having an improved ability to co-metabolize in medium both fructose and glucose are described. The recombinant microorganisms comprise a promoter operably linked to a native or non-native nucleotide sequence that encodes a fucose: H+ symporter polypeptide. The recombinant microorganisms are useful for the production of a variety of products including glycerol and glycerol derivatives such as 1,3-propanediol and 3-hydroxypropionic acid.Type: GrantFiled: October 18, 2010Date of Patent: October 7, 2014Assignee: E I du Pont de Nemours and CompanyInventors: Andrew C. Eliot, Anthony A. Gatenby, John S. Chapman
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Publication number: 20140273129Abstract: Provided herein are glycerol-3-phosphate dehydrogenase (GPD) enzymes with increased KM for NADH and GPD enzymes with substantially the same affinity for NADH and NADPH and/or are feedback inhibited by glycerol-3-phosphate. Also provided herein are recombinant microorganisms comprising a heterologous gene encoding GPD and a deletion or disruption in an endogenous gene encoding GPD. Also provided are recombinant microorganisms comprising a heterologous gene encoding GPD and a butanol biosynthetic pathway. Further provided are methods of producing butanol comprising providing the recombinant microorganisms described herein and contacting the recombinant microorganism with at least one fermentable carbon substrate under conditions wherein butanol is produced.Type: ApplicationFiled: March 13, 2014Publication date: September 18, 2014Applicant: BUTAMAX ADVANCED BIOFUELS LLCInventors: Ritu Bhalla, Gopal K. Chotani, Michael Dauner, Mark J. Nelson, Daniel P. O'keefe, Caroline M. Peres, Jahnavi Chandra Prasad, Jean-Francois Tomb
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Publication number: 20140273110Abstract: Bacteria that run the beta oxidation cycle in reverse anabolic direction are provided, along with many novel primers to start the reverse cycle, pathways to make such primers, and a large variety of products produced thereby. Methods for making desired product by using such primers in the reverse pathway are also disclosed.Type: ApplicationFiled: March 6, 2014Publication date: September 18, 2014Applicant: William Marsh Rice UniversityInventors: Ramon GONZALEZ, James M. CLOMBURG
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Publication number: 20140255996Abstract: A method for secretory production of a heterologous protein is provided by developing a novel technique for improving ability of a coryneform bacterium to produce a heterologous protein by secretory production. By utilizing a coryneform bacterium having an ability to produce a heterologous protein by secretory production which has been modified so that the activity of a penicillin-binding protein is reduced and in which the activity of a cell surface layer protein has been reduced as an expression host, a heterologous protein is produced by secretory production.Type: ApplicationFiled: April 29, 2014Publication date: September 11, 2014Applicant: AJINOMOTO CO., INC.Inventors: Yoshihiko Matsuda, Hiroshi Itaya, Yoshimi Kikuchi, Haruki Beppu, Jurgis Antanas Vladovich Jomantas, Ekaterina Aleksandrovna Kutukova
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Publication number: 20140256904Abstract: The subject of the invention is a biotechnological process for the production of ?-functionalized carboxylic acids and of ?-functionalized carboxylate esters from simple carbon sources.Type: ApplicationFiled: August 15, 2012Publication date: September 11, 2014Applicant: Evonik Degussa GmbHInventors: Steffen Schaffer, Nicole Decker, Jasmin Gielen, Harald Haeger, Thomas Haas, Markus Poetter, Hans-Georg Hennemann, Mirja Wessel, Michael Volland
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Publication number: 20140255302Abstract: The disclosure provides TNF-like ligand 1a (TL1a)-binding proteins comprising an antigen binding domain of an antibody which binds specifically to TL1a and inhibits interaction of TL1a and Death Receptor 3 (DR3) and which does not inhibit the interaction of TL1a and Decoy Receptor 3 (DcR3). The disclosure also provides uses of the TL1a-binding proteins.Type: ApplicationFiled: March 28, 2014Publication date: September 11, 2014Applicant: Teva Pharmaceuticals Australia Pty LtdInventors: Lynn Dorothy Poulton, Adam Clarke, Andrew James Pow, Debra Tamvakis, George Kopsidas, Anthony Gerard Doyle, Philip Anthony Jennings, Matthew Pollard
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Publication number: 20140256008Abstract: The present invention concerns a method for the biological preparation of prenol comprising culturing a microorganism genetically modified for the bioproduction of prenol, wherein the microorganism comprises a metabolic pathway for conversion of 3-methylcrotonyl-CoA into prenol by the action of an alcool dehydrogenase enzyme and of an aldehyde dehydrogenase enzyme.Type: ApplicationFiled: October 11, 2011Publication date: September 11, 2014Applicant: METABOLIC EXPLORERInventors: Cedric Boisart, SR., Guillaume Letellier
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Publication number: 20140256005Abstract: The present invention relates to a novel method for the fermentative production of gamma-aminobutyric acid (GABA) by cultivating a recombinant microorganism expressing an enzyme having a glutamate decarboxylase activity. The present invention also relates to corresponding recombinant hosts, recombinant vectors, expression cassettes and nucleic acids suitable for preparing such hosts as well as to a method for preparing polyamides making use of GABA as obtained fermentative production.Type: ApplicationFiled: May 29, 2014Publication date: September 11, 2014Applicant: BASF SEInventors: Oskar Zelder, Weol Kyu Jeong, Corinna Klopprogge, Andrea Herold, Hartwig Schröder
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Publication number: 20140256930Abstract: An object of the present invention is to provide a method for converting the coenzyme dependency of enzymes of the medium-chain dehydrogenase/reductase (MDR) family. A further object of the present invention is to provide enzyme variants of the MDR family whose coenzyme dependency is converted by the conversion method and a method for enzymatically producing optically active alcohols using the enzymes. The present inventors developed a novel enzyme conversion method for converting the coenzyme dependency of enzymes of the MDR family, rationally designed enzyme variants that are altered by the enzyme conversion method to be able to use NADPH as a coenzyme from a useful enzyme of the MDR family that uses NADH as a coenzyme, and actually provide variants having such an ability.Type: ApplicationFiled: June 27, 2012Publication date: September 11, 2014Applicant: KANEKA CORPORATIONInventors: Shinichi Yoshida, Shunichi Taira, Masakatsu Nishihachijyo, Masutoshi Nojiri, Shigeru Kawano
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Publication number: 20140248673Abstract: This document describes biochemical pathways for producing pimelic acid, 7-hydroxyheptanoic acid, 7-aminoheptanoic acid, heptamethylenediamine or 1,7-heptanediol by forming two terminal functional groups, comprised of carboxyl, amine or hydroxyl group, in a C7 aliphatic backbone substrate. These pathways, metabolic engineering and cultivation strategies described herein rely on the CoA-dependent elongation enzymes or analog enzymes associated with the carbon storage pathways from polyhydroxyalkanoate accumulating bacteria.Type: ApplicationFiled: December 13, 2013Publication date: September 4, 2014Applicant: INVISTA North America S.á r.I.Inventors: Adriana Leonora Botes, Alex Van Eck Conradie
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Publication number: 20140242655Abstract: Embodiments of the present invention relate to methods for the biosynthesis of di- or trifunctional C7 alkanes in the presence of isolated enzymes or in the presence of a recombinant host cell expressing those enzymes. The di- or trifunctional C7 alkanes are useful as intermediates in the production of nylon-7, nylon-7,x, nylon-x,7, and polyesters.Type: ApplicationFiled: June 29, 2012Publication date: August 28, 2014Applicant: INVISTA TECHNOLOGIES S.A R.L.Inventors: Paul S. Pearlman, Changlin Chen, Adriana Leonora Botes, Alex Van Eck Conradie, Benjamin D. Herzog
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Publication number: 20140242704Abstract: The present invention relates to the use of nucleic acid sequences for regulating the transcription and expression of genes, the novel promoters and expression units themselves, methods for altering or causing the transcription rate and/or expression rate of genes, expression cassettes comprising the expression units, genetically modified microorganisms with altered or caused transcription rate and/or expression rate, and methods for preparing biosynthetic products by cultivating the genetically modified microorganisms.Type: ApplicationFiled: May 8, 2014Publication date: August 28, 2014Applicant: BASF SEInventors: Oskar Zelder, Corinna Klopprogge, Burkhard Kröger, Hartwig Schröder, Stefan Haefner
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Publication number: 20140234924Abstract: The present invention provides methods of designing and generating glycerol dehydrogenase (GlyDH) variants that have altered function as compared to a parent polypeptide. The present invention further provides nucleic acids encoding GlyDH polypeptide variants having altered function as compared to the parent polypeptide. Host cells comprising polynucleotides encoding GlyDH variants and methods of producing lactic acids are also provided in various aspects of the invention.Type: ApplicationFiled: October 4, 2012Publication date: August 21, 2014Inventors: Qingzhao Wang, Keelnatham T. Shanmugam, Lonnie O'Neal Ingram
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Publication number: 20140234256Abstract: Genetically engineered cells and microorganisms are provided for preventing or ameliorating diseases through genetically engineered quorum signaling. Therapeutic methods for using the cells and microorganisms to prevent or ameliorate diseases are also provided. The genetically engineered cells or microorganisms can be engineered to express a signal and used to interrupt the signaling-dependent virulence of an invading pathogen. The cells or microorganisms can be used to provide signal-dependent expression of a desirable gene in order to interrupt, prevent, and/or ameliorate a disease of mammals, such as parasitic diseases, infectious diseases, autoimmune diseases and genetic disorders.Type: ApplicationFiled: January 9, 2014Publication date: August 21, 2014Applicant: Cornell UniversityInventors: John C. March, Franklin Faping Duan
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Publication number: 20140227764Abstract: Proteases that comprise an amino acid sequence that is at least 70% identical to the amino acid sequence indicated in SEQ ID NO. 1 over its entire length and that comprise, in the count in accordance with SEQ ID NO. 1, the amino acid substitution R99E or R99D in combination with at least two further amino acid substitutions that are selected from the group consisting of S3T, V4I, and V199I, display very good cleaning performance in particular on blood-containing stains, as well as very good temperature stability.Type: ApplicationFiled: April 25, 2014Publication date: August 14, 2014Applicant: Henkel AG & Co. KGaAInventors: Hendrik Hellmuth, Marion Merkel, Brian Laufs, Susanne Wieland, Timothy O'Connell, Susanne Tondera, Thomas Weber
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Publication number: 20140227747Abstract: A transformant obtainable by introducing one or more of the following DNAs (a), (b), and (c) into a coryneform bacterium as a host. (a) A DNA which encodes acetohydroxy acid synthase derived from Corynebacterium glutamicum and which has a mutation changing the glycine at position 156 to glutamic acid (G156E) in an amino acid sequence encoded by the DNA, or an analog thereof. (b) A DNA which encodes acetohydroxy acid isomeroreductase derived from Corynebacterium glutamicum and which has mutations changing the serine at position 34 to glycine (S34G), the leucine at position 48 to glutamic acid (L48E), and the arginine at position 49 to phenylalanine (R49F) in an amino acid sequence encoded by the DNA, or an analog thereof. (c) A DNA which encodes leucine dehydrogenase derived from Lysinibacillus sphaericus, or an analog thereof.Type: ApplicationFiled: August 21, 2012Publication date: August 14, 2014Applicant: RESEARCH INSTITUTE OF INNOVATIVE TECHNOLOGY FOR THE EARTHInventors: Hideaki Yukawa, Masayaki Inui
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Patent number: 8802393Abstract: The present invention relates to a thermophilic arabinose isomerase and a method of manufacturing tagatose using the same, and more precisely, a gene encoding arabinose isomerase originating from the thermophile Thermotoga neapolitana DSM 5068, a recombinant expression vector containing the gene, a method of preparing a food grade thermophilic arabinose isomerase from the recombinant GRAS (Generally Recognized As Safe) strain transformed with the said expression vector, and a method of preparing tagatose from galactose using the said enzyme.Type: GrantFiled: October 17, 2008Date of Patent: August 12, 2014Assignee: CJ Cheiljedang CorporationInventors: Seong-bo Kim, Young-mi Lee, Seung-won Park, Jung-hoon Kim, Sang-hoon Song, Kang-pyo Lee, Hye-won Kim, Hye-jin Choi
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Patent number: 8802402Abstract: A substitution mutation that improves polymerization activity of a polyhydroxyalkanoic acid synthase is identified. At least 1 amino acid residue selected from the group consisting of a histidine residue at position 17, a proline residue at position 71, a valine residue at position 131, a methionine residue at position 205, a leucine residue at position 230, and a proline residue at position 239 of a polyhydroxyalkanoic acid synthase derived from Alcanivorax borkumensis is subjected to substitution mutation with another amino acid.Type: GrantFiled: July 14, 2010Date of Patent: August 12, 2014Assignee: Toyota Jidosha Kabushiki KaishaInventors: Masayoshi Muramatsu, Hiromi Kambe, Masakazu Ito
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Publication number: 20140220628Abstract: The aim is to increase the protein product yield in microbial fermentation. This is achieved by a method which introduces into a microorganism not only a first expression construct which encodes the protein, but also a second expression construct which encodes an auxiliary protease which differs from the protein, is proteolytically active and which comprises an amino acid sequence which is at least 50% identical to the amino acid sequence indicated in SEQ ID NO. 1.Type: ApplicationFiled: April 5, 2012Publication date: August 7, 2014Applicant: BASF SEInventors: Lukas Maksym, Ramona Knab, Stefan Evers, Karl-Heinz Maurer, Johannes Bongaerts
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Publication number: 20140212886Abstract: A vector that allows for easy accomplishment of a variety of cloning, and use of the vector for measurement of the transcription-inducing activity of a promoter or production of a desired gene product in Corynebacterium.Type: ApplicationFiled: January 24, 2014Publication date: July 31, 2014Inventors: Woo-yong LEE, Young-min Lee, Jae-chan Park, Jin-hwan Park, Yeo-ju Song
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Publication number: 20140212934Abstract: Provided herein are methods for the production of difunctional alkanes in microorganisms. Also provided are enzymes and nucleic acids encoding such enzymes, associated with the difunctional alkane production from carbohydrates feedstocks in microorganisms. The invention also provides recombinant microorganisms and metabolic pathways for the production of difunctional alkanes.Type: ApplicationFiled: April 9, 2014Publication date: July 31, 2014Applicant: Celexion, LLCInventors: Brian M. Baynes, John Michael Geremia, Shaun M. Lippow
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Publication number: 20140206068Abstract: A process for the fermentative preparation of L-ornithine using microorganisms characterized by an increased export of the amino acid.Type: ApplicationFiled: February 20, 2014Publication date: July 24, 2014Applicant: Evonik Degussa GmbHInventors: Wilfried CLAES, Robert Gerstmeir
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Publication number: 20140206069Abstract: Aspects of the invention relate to methods for the production of difunctional alkanes in host cells. In particular, aspects of the invention describe components of genes associated with the difunctional alkane production from carbohydrate feedstocks in host cells. More specifically, aspects of the invention describe metabolic pathways for the production of adipic acid, aminocaproic acid, caprolactam, and hexamethylenediamine via 2-ketopimelic acid.Type: ApplicationFiled: March 20, 2014Publication date: July 24, 2014Applicant: Celexion, LLCInventors: Brian M. Baynes, John Michael Geremia
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Publication number: 20140199732Abstract: The present invention relates to a D-psicose 3-epimerase variant with improved thermostability by substituting an amino acid at a specific position of an amino acid sequence of a wild type D-psicose 3-epimerase. Further, the present invention provides a recombinant expression vector including a gene of the D-psicose 3-epimerase variant, and a recombinant strain transformed with the recombinant expression vector. Furthermore, the present invention provides an immobilized reactor prepared using the D-psicose 3-epimerase variant or the recombinant strain, and a method of continuously producing D-psicose using the immobilized reactor.Type: ApplicationFiled: August 21, 2012Publication date: July 17, 2014Applicant: CJ CHEILJEDANG CORPORATIONInventors: Yang Hee Kim, Jin Ha Kim, Young Mi Lee, Young Ho Hong, Min Hae Kim, Seong Bo Kim, Seung Won Park, Seung Hyun Oh, Deok Kun Oh, Jin Geun Choi
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Publication number: 20140199737Abstract: This document describes biochemical pathways for producing adipic acid, 6-aminohexanoic acid, 6-hydroxhexanoic acid, hexamethylenediamine, caprolactam, or 1,6-hexanediol by forming one or two terminal functional groups, comprised of carboxyl, amine or hydroxyl group, in a C6 aliphatic backbone substrate. These pathways, metabolic engineering and cultivation strategies described herein rely on the enzymes or homologs accepting methyl ester shielded dicarboxylic acid substrates.Type: ApplicationFiled: December 23, 2013Publication date: July 17, 2014Inventors: Adriana Leonora Botes, Alex Van Eck Conradie
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Publication number: 20140193862Abstract: This document describes biochemical pathways for producing pimelic acid, 7-aminoheptanoic acid, 7-hydroxyheptanoic acid, heptamethylenediamine or 1,7-heptanediol by forming two terminal functional groups, comprised of carboxyl, amine or hydroxyl group, in a C7 aliphatic backbone substrate. These pathways, metabolic engineering and cultivation strategies described herein rely on the carbon chain elongation enzymes or homologs thereof associated with the cyclohexane carboxylate biosynthesis from Syntrophus aciditrophicus or 2-aminoadipate lysine biosynthesis.Type: ApplicationFiled: December 23, 2013Publication date: July 10, 2014Applicant: INVISTA North America S.á r.I.Inventors: Adriana Leonora Botes, Alex Van Eck Conradie, Changlin Chen, Paul S. Pearlman
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Publication number: 20140193865Abstract: This document describes biochemical pathways for producing pimelic acid, 7-aminoheptanoic acid, 7-hydroxyheptanoic acid, heptamethylenediamine or 1,7-heptanediol by forming two terminal functional groups, comprised of carboxyl, amine or hydroxyl group, in a C7 aliphatic backbone substrate. These pathways, metabolic engineering and cultivation strategies described herein rely on the fatty acid synthesis pathway and oxidative cleavage of long chain acyl-[acp] intermediates by a monooxgenase (e.g., cytochrome P450) such as that encoded by BioI from microorganisms such as Bacillus subtillis.Type: ApplicationFiled: December 23, 2013Publication date: July 10, 2014Applicant: INVISTA North America S.á r.I.Inventors: Adriana Leonora Botes, Alex Van Eck Conradie, Changlin Chen, Paul S. Pearlman
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Publication number: 20140193861Abstract: This document describes biochemical pathways for producing pimelic acid, 7-aminoheptanoate, 7-hydroxyheptanoate, heptamethylenediamine, or 1,7-heptanediol by forming two terminal functional groups, comprised of carboxyl, amine or hydroxyl group, in a C7 aliphatic backbone substrate produced from chorismate or benzoate. These pathways, metabolic engineering and cultivation strategies described herein rely on the anaerobic benzoyl-CoA degradation pathway enzymes.Type: ApplicationFiled: December 23, 2013Publication date: July 10, 2014Applicant: INVISTA North America S.á.r.l.Inventors: Adriana Leonora Botes, Alex Van Eck Conradie, Changlin Chen, Paul S. Pearlman
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Publication number: 20140193863Abstract: This document describes biochemical pathways for producing pimelic acid, 7-aminoheptanoic acid, 7-hydroxyheptanoic acid, heptamethylenediamine or 1,7-heptanediol by forming one or two terminal functional groups, each comprised of carboxyl, amine or hydroxyl group, in a C7 aliphatic backbone substrate. These pathways, metabolic engineering and cultivation strategies described herein rely on the C1 elongation enzymes or homolog associated with coenzyme B biosynthesis.Type: ApplicationFiled: December 23, 2013Publication date: July 10, 2014Applicant: INVISTA North America S.á r.I.Inventors: Adriana Leonora Botes, Alex Van Eck Conradie, Changlin Chen, Paul S. Pearlman
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Patent number: 8771998Abstract: The present invention relates to a novel method for the fermentative production of gamma-aminobutyric acid (GABA) by cultivating a recombinant microorganism expressing an enzyme having a glutamate decarboxylase activity. The present invention also relates to corresponding recombinant hosts, recombinant vectors, expression cassettes and nucleic acids suitable for preparing such hosts as well as to a method for preparing polyamides making use of GABA as obtained fermentative production.Type: GrantFiled: February 20, 2009Date of Patent: July 8, 2014Assignee: BASF SEInventors: Oskar Zelder, Weol Kyu Jeong, Corinna Klopprogge, Andrea Herold, Hartwig Schröder
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Publication number: 20140186904Abstract: This document describes biochemical pathways for producing pimelic acid, 7-aminoheptanoic acid, 7-hydroxyheptanoic acid, heptamethylenediamine or 1,7-heptanediol by forming two terminal functional groups, comprised of carboxyl, amine or hydroxyl group, in a C7 aliphatic backbone substrate. These pathways, metabolic engineering and cultivation strategies described herein rely on enzymes or homologs accepting methyl ester shielded dicarboxylic acid substrates.Type: ApplicationFiled: December 23, 2013Publication date: July 3, 2014Applicant: INVISTA North America S.á r.l.Inventors: Adriana Leonora Botes, Alex Van Eck Conradie, Changlin Chen, Paul S. Pearlman
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Publication number: 20140186910Abstract: Dihydroxy-acid dehydratase (DHAD) variants that display increased DHAD activity are disclosed. Such enzymes can result in increased production of compounds from DHAD requiring biosynthetic pathways. Also disclosed are isolated nucleic acids encoding the DHAD variants, recombinant host cells comprising the isolated nucleic acid molecules, and methods of producing butanol.Type: ApplicationFiled: December 27, 2013Publication date: July 3, 2014Applicant: ButamaxTM Advanced Biofuels LLCInventors: LORI ANN MAGGIO-HALL, BRIAN JAMES PAUL, STEVEN CARY ROTHMAN, RICK W. YE
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Publication number: 20140186902Abstract: This document describes biochemical pathways for producing adipic acid, caprolactam, 6-aminohexanoic acid, 6-hydroxyhexanoic acid, hexamethylenediamine or 1,6-hexanediol by forming two terminal functional groups, comprised of carboxyl, amine or hydroxyl groups, in a C6 aliphatic backbone substrate. These pathways, metabolic engineering and cultivation strategies described herein rely on CoA-dependent elongation enzymes or analogues enzymes associated with the carbon storage pathways from polyhydroxyalkanoate accumulating bacteria.Type: ApplicationFiled: December 13, 2013Publication date: July 3, 2014Applicant: INVISTA North America S. a r.l.Inventors: Adriana Leonora Botes, Alex Van Eck Conradie
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Publication number: 20140178961Abstract: The present invention relates to a construct for accomplishing fixation of carbon dioxide and/or reduction of carbon dioxide emission in a heterotrophic microorganism (for example, a heterotrophic fermentation strain, such as E. coli), a vector comprising the construct, a heterotrophic microorganism (for example, a heterotrophic fermentation strain, such as E. coli) comprising the construct or being transformed with the vector, and a method for fixing carbon dioxide and/or reducing carbon dioxide emission in a heterotrophic microorganism (for example, a heterotrophic fermentation strain, such as E. coli).Type: ApplicationFiled: December 19, 2013Publication date: June 26, 2014Applicant: SHELL OIL COMPANYInventors: Xuefeng LU, Liu YANG, Zhengxu GAO, Xiaoming TAN
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Publication number: 20140178949Abstract: The present invention relates to a method for preparing an adipate ester or thioester. The invention further relates to a method for preparing adipic acid from said ester or thioester. Further the invention provides a number of methods for preparing an intermediate for said ester or thioester. Further the invention relates to a method for preparing 6-amino caproic acid (6-ACA), a method for preparing 5-formyl valeric acid (5-FVA), and a method for preparing caprolactam. Further, the invention relates to a host cell for use in a method according to the invention.Type: ApplicationFiled: November 15, 2013Publication date: June 26, 2014Applicant: DSM IP ASSETS B.V.Inventors: LIANG WU, AXEL CHRISTOPH TREFZER, STEFAAN MARIE ANDRE DE WILDEMAN, MARCO ALEXANDER VAN DEN BERG
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Publication number: 20140170732Abstract: From a bacterial strain isolated from an environmental sample, after enrichment in medium containing 1-butanol as the carbon source, a new enzyme with butanol dehydrogenase activity was identified. The enzyme can convert butyraldehyde to 1-butanol, isobutyraldehyde to isobutanol, as well as 2-butanone to 2-butanol and thus is useful for biosynthesis of butanol in recombinant microbial hosts producing these substrates. The encoding gene, named sadB, was isolated from the strain identified as an isolate of Achromobacter xylosoxidans.Type: ApplicationFiled: February 13, 2014Publication date: June 19, 2014Applicant: BUTAMAX ADVANCED BIOFUELS LLCInventors: Michael G. Bramucci, Andrew C. Eliot, Lori Ann Maggio-Hall, Charles E. Nakamura
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Publication number: 20140170731Abstract: Embodiments herein generally relate to methods, compositions, systems and uses for enabling bio-production of or increasing bio-production of alcohol molecules by microorganisms. Certain embodiments relate to compositions and methods enabling or increasing the bio-production of 4-carbon alcohol molecules by bacteria. In some embodiments, compositions and methods relate to introducing isobutyryl-CoA isomerase to a culture of microorganisms to enable or increase the bio-production of four-carbon alcohols. Variations of biosynthesis pathways for microbial bio-production of butanol and/or isobutanol are provided.Type: ApplicationFiled: December 13, 2013Publication date: June 19, 2014Applicant: OPX BIOTECHNOLOGIES, INC.Inventor: Michael D. Lynch
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Patent number: 8753850Abstract: The invention provides a non-naturally occurring microbial organism having a 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in the respective 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway. The invention additionally provides a method for producing 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid. The method can include culturing a 6-aminocaproic acid, caprolactam or hexametheylenediamine producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding a 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway enzyme in a sufficient amount to produce the respective product, under conditions and for a sufficient period of time to produce 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid.Type: GrantFiled: July 13, 2012Date of Patent: June 17, 2014Assignee: Genomatica, Inc.Inventors: Mark J. Burk, Anthony P. Burgard, Robin E. Osterhout, Priti Pharkya
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Patent number: 8753849Abstract: The present invention provides a bacterium which has an ability to produce a useful metabolite derived from acetyl-coenzyme A, such as L-glutamic acid, L-glutamine, L-proline, L-arginine, L-leucine, L-cysteine, succinate, and polyhydroxybutyrate, wherein said bacterium is modified so that activities of D-xylulose-5-phosphate phosphoketolase and/or fructose-6-phosphate phosphoketolase are enhanced. The present invention also provides a method for producing the useful metabolite using the bacterium.Type: GrantFiled: February 22, 2013Date of Patent: June 17, 2014Assignee: Ajinomoto Co., Inc.Inventors: Yury Ivanovich Kozlov, Akito Chinen, Hiroshi Izui, Yoshihiko Hara, Hisashi Yasueda, Konstantin Vyacheslavovich Rybak, Ekaterina Aleksandrovna Slivinskaya, Joanna Yosifovna Katashkina
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Publication number: 20140162344Abstract: Disclosed herein are methods of producing alcohol esters during a fermentation by providing alcohol-producing microorganisms which further comprise an engineered polynucleotide encoding a polypeptide having lipase activity.Type: ApplicationFiled: February 14, 2014Publication date: June 12, 2014Applicant: BUTAMAX ADVANCED BIOFUELS LLCInventors: Robert DICOSIMO, Arthur Leo Kruckeberg, Thomas Edwin Van Aken