Abstract: The present invention relates to a novel aspartokinase derived from a Coryneform bacterium; a DNA encoding the enzyme; a recombinant DNA containing the above DNA; a Coryneform bacterium having the above recombinant DNA or a Coryneform bacterium having the DNA on its chromosome; and a process for producing L-lysine by culturing the above microorganism. Construction has been successfully made of a DNA encoding an aspartokinase freed from concerted feedback inhibition by L-lysine and L-threonine derived from a Corynebacterium and has a nucleotide sequence encoding an amino acid sequence wherein the amino acid residue at position 311 is an amino acid other than Thr in the amino acid sequence shown by SEQ ID NO: 18.

Abstract: Isolated nucleic acid molecules, designated PTS nucleic acid molecules, which encode novel PTS proteins from Corynebacterium glutamicum are described. The invention also provides antisense nucleic acid molecules, recombinant expression vectors containing PTS nucleic acid molecules, and host cells into which the expression vectors have been introduced. The invention still further provides isolated PTS proteins, mutated PTS proteins, fusion proteins, antigenic peptides and methods for the improvement of production of a desired compound from C. glutamicum based on genetic engineering of PTS genes in this organism.

Abstract: A DNA fragment which encodes a polypeptide defined in the following (a) or (b), and a polypeptide defined in the following (c) or (d): (a) a polypeptide which has at least the amino acid sequence of the amino acid numbers 50 to 393 in SEQ ID NO: 2 shown in Sequence Listing, (b) a polypeptide which has at least the amino acid sequence of the amino acid numbers 50 to 393 in SEQ ID No: 2 shown in Sequence Listing including substitution, deletion, insertion, addition, or inversion of one or several amino acids, and can constitute a protein having a carbamoyl-phosphate synthetase activity with a large subunit of carbamoyl-phosphate synthetase having the amino acid sequence comprising at least the amino acid numbers 55 to 1113 of SEQ ID NO: 3, (c) a polypeptide which has the amino acid sequence comprising at least the amino acid numbers 55 to 1113 of SEQ ID NO: 3 shown in Sequence Listing, (d) a polypeptide which has the amino acid sequence comprising at least the amino acid numbers 55 to 1113 of SEQ ID NO: 3 sh

Abstract: The present invention relates to polynucleotides corresponding to the luxR gene and which encode a LuxR transcriptional activator, methods of producing L-amino acids, and methods of screening for polynucleotides which encode proteins having LuxR transcriptional activation activity. The invention also relates to isolating the luxR gene and which encode a LuxR transcriptional activator from Corynebacterium glutamicum.

Abstract: The invention relates to isolated nucleotide sequences from Coryneform bacteria which code for the pck gene encoding the enzyme phosphoenol pyruvate carboxykinase (PEP carboxykinase). The invention also relates a process for the fermentative preparation of L-amino acids, in particular L-lysine, L-threonine, and L-glutamate by attenuation of the pck gene.

Abstract: The invention relates to L-lysine-producing strains of corynebacteria with enhanced pyc gene (pyruvate carboxylase gene), in which strains additional genes, chosen from the group consisting of the dapA gene (dihydrodipicolinate synthase gene), the lysC gene (aspartate kinase gene), the lysE gene (lysine export carrier gene) and the dapB gene (dihydrodipicolinate reductase gene), but especially the dapA gene, are enhanced and, in particular, over-expressed, and to a process for the preparation of L-lysine.

Abstract: The invention pertains to a process for the microbial production of amino acids. The process involves boosting the export carrier activity and/or export gene expression of a microorganism which produces the desired amino acid. It was found that a single specific gene is responsible for the export of a given amino acid, and on that basis, a process for the microbial production of amino acids, involving the controlled boosting of the export gene expression and/or export carrier activity of a microorganism was developed, which produces the amino acid. The boosted expression or activity of the export carrier resulting from this process increases the secretion rate and thus increases transport of the desired amino acid.

Abstract: A coryneform bacterium which has enhanced intracellular pyruvate carboxylase activity obtained by increasing copy number of a gene encoding the intracellular pyruvate carboxylase, or by enhancing function of a expression regulatory sequence for the gene, and has L-glutamic acid-producing ability is cultured in a medium so that L-glutamic acid should be produced and accumulated in the culture, and L-glutamic acid is collected from the culture.

Abstract: Alleles of the lysC gene from corynebacteria that code for desensitized aspartokinases, and to processes for the preparation of L-lysine using bacteria containing these alleles.

Abstract: The present invention provides isolated polynucleotides containing a polynucleotide sequence which is: a) polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, or b) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70% to the amino acid sequence of SEQ ID No. 2, or c) polynucleotide which is complementary to the polynucleotides of a) or b), or d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and a process for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the ccpA1 gene is present in attenuated form, and the use of the polynucleotide sequences as hybridization probes.

Abstract: The present invention relates, in general, to a method of producing L-amino acids comprising culturing altered bacterial cells having increased amounts of NADPH as compared to unaltered bacterial cells whereby L-amino acids yields from said altered bacterial cells are greater than yields from unaltered bacterial cells. The invention also relates to a gene encoding phosphoglucoisomerase.

Abstract: The invention relates to an isolated polynucleotide comprising a polynucleotide sequence chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70 % to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, b) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70 % to the amino acid sequence of SEQ ID No. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), a process for the fermentative preparation of L-amino acids with enhancement of the acp gene and the use of the polynucleotide as a primer or hybridization probe.

Abstract: The present invention relates to polynucleotides that encode proteins having OpcA enzymatic activity. These polynucleotides can be used for increasing lysine biosynthesis in Coryneform glutamicum.

Abstract: This invention relates to a process for producing a sugar nucleotide, in which a) a culture broth of a microorganism capable of producing NTP from a nucleotide precursor, or a treated product of the culture broth, and b) a culture broth of a microorganism capable of producing a sugar nucleotide from a sugar and NTP, or a treated product of the culture broth, are used as enzyme sources; a process for producing a complex carbohydrate, in which the above-described a) and b) and c) a culture broth of a microorganism, an animal cell or an insect cell capable of producing a complex carbohydrate from a sugar nucleotide and a complex carbohydrate precursor, or a treated product of the culture broth, are used as enzyme sources; a process for producing a complex carbohydrate, in which a culture broth of a microorganism, an animal cell or an insect cell capable of producing a complex carbohydrate from a sugar nucleotide and a complex carbohydrate precursor, or a treated product of the culture broth, is as an enzyme sour

Abstract: The invention relates to an isolated polynucleotide containing a polynucleotide sequence selected from the group comprising a) polynucleotide which is at least 70% identical to a polynucleotide which codes for a polypeptide containing the amino acid sequence of SEQ ID no. 2, b) polynucleotide which codes for a polypeptide containing an amino acid sequence which is at least 70% identical to the amino acid sequence of SEQ ID no.2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide containing at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and a process for the fermentative production of L-amino acids with enhancement of the ptsH gene coding for component H of the phosphotransferase system, and the use of the above polynucleotides as primer or hybridisation probe.

Abstract: The present invention relates to polynucleotides corresponding to the lysR3 gene and which encode a LysR3 transcriptional regulator, methods of producing L-amino acids, and methods of screening for polynucleotides which encode proteins having LysR3 transcriptional regulator activity.

Abstract: The invention relates to nucleotide sequences coding for the accDA gene and to a process for the preparation of L-amino acids, especially L-lysine, by fermentation using corynebacteria in which the accDA gene is amplified.

Abstract: The present invention is directed to nucleotide sequences coding for phosphofructokinase which have been derived from Corynebacterium glutamicum.

Abstract: The present invention relates to polynucleotides corresponding to the rpoB gene and which encode the &bgr;-subunit of RNA polymerase B, methods of producing L-amino acids, and methods of screening for polynucleotides which encode proteins having activity of the &bgr;-subunit of RNA polymerase B.

Abstract: The invention provides nucleotide sequences from Coryneform bacteria which code for the RodA protein and a process for the fermentative preparation of amino acids using bacteria in which the rodA gene is enhanced.

Abstract: An isolated polynucleotide comprising a polynucleotide sequence selected from the group consisting of a) polynucleotide which is at least 70% identical to a polynucleotide that codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, b) polynucleotide which codes for a polypeptide that comprises an amino acid sequence which is at least 70% identical to the amino acid sequence of SEQ ID No. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and a process for the fermentative preparation of L-amino acids using coryneform bacteria, in which at least the sahH gene is present in enhanced form, and the use of polynucleotides which contain the sequences according to the invention as hybridization probes.

Abstract: The present invention provides an isolated polynucleotide containing a polynucleotide sequence selected from the group a) polynucleotide which is at least 70% identical to a polynucleotide which codes for a polypeptide containing the amino acid sequence of SEQ ID no. 2, b) polynucleotide which codes for a polypeptide which contains an amino acid sequence which is at least 70% identical to the amino acid sequence of SEQ ID no. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide containing at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and a process for the fermentative production of L-amino acids with enhancement of the glbO gene which codes for the haemoglobin-like protein and the use of the above polynucleotides as a primer or hybridization probe.

Abstract: This invention relates to novel polynucleotide sequences encoding the histidine kinase luminescence expression sensor (luxS) gene from Corynebaclerium glutamicum, probes to the novel polynucleotide sequences encoding the luxS gene, vector and host cells containing the novel luxS polynucleotide sequences, the encoded Lux S polypeptide, and a process for the fermentative preparation of amino acids using bacteria which the luxS gene is attenuated.

Abstract: The invention relates to L-lysine-producing strains of corynebacteria with enhanced lysE gene (lysine export carrier gene), in which strains additional genes chosen from the group comprising the dapA gene (dihydrodipicolinate synthase gene), the lysC gene (aspartate kinase gene), the dapB gene (dihydrodipicolinate reductase gene) and the pyc gene, but especially the dapA gene and the lysC gene (aspartate kinase gene), are enhanced and, in particular, over-expressed, and to a process for the preparation of L-lysine.

Abstract: The invention provides nucleotide sequences from Coryneform bacteria coding for the ChrS protein and a process for the fermentative preparation of amino acids using bacteria in which the ChrS protein is attenuated.

Abstract: A nutrient formulation including moisture, a coloring agent, a palatability modifier, and/or an adjuvant which is designed for use in poultry and other animals, and a method of feeding it which improves subsequent livability, cumulative feed efficiency, weight gain, and resistance to disease challenge or other stresses is disclosed.

Abstract: DNA encoding a novel protein from Mycobacterium aurum SC-S423 capable of converting acetophenone to an optically active 1-phenylethlamine in the presence of a racemic mixture of sec-butylamine is provided, along with a vector and host cell comprising the same and a method of producing the same.

Abstract: The invention relates to an isolated polynucleotide having a polynucleotide sequence which codes for the sigH gene, and a host-vector system having a coryneform host bacterium in which the sigH gene is present in attenuated form and a vector which carries at least the sigH gene according to SEQ ID No 1, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.

Abstract: The present invention provides a process for highly efficiently producing ethanol at a high productivity consisting of using a coryneform bacterium which has been transformed by a DNA containing a gene expressing pyruvate decarboxylase activity and, if desired, a gene expressing alcohol dehydrogenase activity under a regulatory sequence allowing for the expression, under ethanol production conditions wherein this bacterium does not substantially proliferate to produce ethanol.

Abstract: According to the present invention, an isolated and purified DNA sequence which encodes a lantibiotic, mutacin I, is disclosed. The nucleic acid sequence is set forth in SEQ ID No: 1 and the amino acid sequence is set forth in SEQ ID No: 2.

Abstract: The present invention relates to polynucleotides corresponding to the nadA gene and which encode the quinolinate synthetase A protein, methods of producing nicotinic acid or nicotinic acid derivatives, and methods of screening for polynucleotides which encode proteins having quinolinate synthetase A activity.

Abstract: The invention relates to polynucleotides corresponding to the ccpA2 gene and which encode a CcpA2 catabolite control protein, methods of producing L-amino acids, and methods of screening for polynucleotides which encode proteins having CcpA2 catabolite control activity.

Abstract: The present invention relates to polynucleotides corresponding to the plsC gene and which encode 1-acyl-SN-glycerol-3-phosphate acyltransferase, methods of producing L-amino acids, and methods of screening for polynucleotides which encode proteins having 1-acyl-SN-glycerol-3-phosphate acyltransferase activity.

Abstract: The present invention provides nucleotide sequences from Coryneform bacteria which code for the PtsI protein and a process for the fermentative preparation of amino acids using bacteria in which the ptsI gene is enhanced.

Abstract: The present invention relates to a process for producing 5′-inosinic acid or 5′-guanylic acid for use in seasonings or the like from inosine or guanosine or a precursor thereof using an adenosine triphosphate (ATP)-regenerating microorganism containing a DNA encoding a protein that has the activity of forming 5′-inosinic acid or 5′-guanylic acid from inosine or guanosine. Further, the present invention relates to a novel protein having the inosine-guanosine kinase activity, a gene encoding said protein, a recombinant DNA containing said gene, and a microorganism which is transformed with said recombinant DNA.

Abstract: The invention relates to a genetically modified coryneform bacterium, the cma gene of which is amplified, and an isolated polynucleotide which codes for cyclopropane-mycolic acid synthase from coryneform bacteria, and also a method for the fermentative preparation of L-amino acids with amplification of the cma gene in the bacteria and the use of the polynucleotide as a primer or hydridization probe.

Abstract: The invention provides a process for the fermentative preparation of L-threonine using Enterobacteriaceae which in particular already produce L-threonine and in which the nucleotide sequence(s) which code(s) for the mqo gene are enhanced, in particular over-expressed.

Abstract: Polynucleotides that contain polynucleotide sequences encoding the sucC and sucD genes, selected from the group a) a polynucleotide that is at least 70% identical to a polynucleotide encoding a polypeptide that contains the amino acid sequence of SEQ ID NO:2, b) a polynucleotide that is at least 70% identical to a polynucleotide encoding a polypeptide that contains the amino acid sequence of SEQ ID NO:3, c) a polynucleotide encoding a polypeptide that contains an amino acid sequence that is at least 70% identical to the amino acid sequence of SEQ ID NO:2, d) a polynucleotide encoding a polypeptide that contains an amino acid sequence that is at least 70% identical to the amino acid sequence of SEQ ID NO:3, e) a polynucleotide that is complementary to one of the polynucleotides of a), b), c) or d), and f) a polynucleotide containing at least 15 successive nucleotides of the polynucleotide sequence of a), b), c), d) or e), a process for the fermentative production of L-amino acids using coryneform bacter

Abstract: The global effect on genes under different environmental conditions can be determined by a comprehensive gene expression profile. The present invention provides a method to monitor the changes in comprehensive cellular gene expression levels at single length resolution by using a high-density microarray prepared with a comprehensive collection of ORFs of a genome. Under different environmental conditions, directly and indirectly affected genes can be detected as the gene expression levels are induced or repressed in comparison to the control.

Abstract: The present invention provides a method of increasing the productivity of a microorganism by improving the assimilation of carbon dioxide. Specifically, the invention provides a polypeptide having phosphoenolpyruvate carboxylase activity which does not require acetyl coenzyme A for activation and is desensitized to feedback inhibition by aspartic acid, and to genes coding for this polypeptide. A gene encoding a PEP carboxylase that is not regulated by acetyl-CoA or aspartic acid can improve carbon flow from the three carbon intermediate PEP to the four carbon intermediate OAA, contribute to compounds derived from OAA, and increase amino acid biosynthesis. The invention further provides recombinant DNA molecules containing these genes, bacteria transformed with these genes, and a method of producing amino acids using the transformed bacteria.

Abstract: The present invention relates to a novel flavoring solution for making pickles having a flavor of foods pickled in rice bran paste (hereinafter referred to as a bran pickles flavoring solution), a clear bran pickles flavoring solution, processes for producing said solutions, a novel microorganism belonging to the genus Corynebacterium used for the production of said flavoring solutions, and a process for producing a solution containing a lactone wherein the lactone is selectively extracted from a culture of a lactone-producing microorganism. The present invention also relates to a novel bacterium of the genus Corynebacterium which has the ability to produce &ggr;-dodecalactone and/or &ggr;-dodecelactone and is useful for the production of said bran pickles flavoring solutions.

Abstract: The present invention is directed to isolated polynucleotides coding for phosphoglucose isomerase (pgi) from coryneform bacteria. In addition, the invention includes methods for increasing the metabolic flux through pentose phosphate cycle of bacteria by reducing or eliminating the activity of pgi. These methods may be used to increase the fermentative production of nucleotides, vitamins and amino acids.

Abstract: A method for producing a fermentative product by utilizing a microorganism, the method comprising culturing the microorganism in a medium to produce and accumulate the fermentative product in the medium, and collecting the fermentative product, wherein the microorganism expresses a heat shock protein derived from a hyperthermophilic archaeon strain KOD-1 in a cell of the microorganism by introduction of a gene coding for the heat shock protein.

Abstract: The present invention is directed to nucleotide sequences coding for the superoxide dismutase (sod) gene from Corynebacterium melassecola. It includes processes for the fermentative preparation of nucleotides, vitamins and L-amino acids using coryneform bacteria in which the sod gene is amplified.

Abstract: This invention relates to a genetically modified coryneform bacterium, the cls gene of which is amplified, and to an isolated polynucleotide, which codes for cardiolipin synthase from coryneform bacteria and to a process for the fermentative production of L-amino acids with amplification of the cls gene in the bacteria and to the use of the polynucleotide as a primer or hybridization probe.

Abstract: A DNA encoding the following protein (A) or (B) which participates in a temperature sensitivity to a surfactant of coryneform bacteria:

Abstract: The present invention is directed to genetic material useful for the preparation of actinol, such as an isolated DNA including a nucleotide sequence coding for an enzyme having levodione reductase activity, a polypeptide encoded by such a DNA, recombinant organisms, and the like. These genetic materials may originate from Corynebacterium, Cellulomonas, Planococcus, Arthrobacter, and the like. The present invention also provides a process for the production of actinol.

Abstract: The present invention relates, in general, to the over-production of L-isoleucine by nonhuman organisms. More specifically, the present invention relates to methods for producing L-isoleucine comprising: (a) growing a transformed nonhuman organism under conditions that provide for synthesis of L-isoleucine, wherein the nonhuman organism comprises one or more copies of a transgene comprising at least one nucleotide sequence encoding catabolic threonine dehydratase; wherein the L-isoleucine is synthesized by the transformed nonhuman organism, the synthesis being greater than that of the corresponding non-transformed nonhuman organism; and (b) recovering the L-isoleucine from the culture medium in which the transformed nonhuman organism was cultured.

Abstract: The L-lysine-producing ability and the L-lysine-producing speed are improved in a coryneform bacterium harboring an aspartokinase in which feedback inhibition by L-lysine and L-threonine is substantially desensitized, by successively enhancing DNA coding for a dihydrodipicolinate reductase, DNA coding for a dihydrodipicolinate synthase, DNA coding for a diaminopimelate decarboxylase, and DNA coding for a diaminopimelate dehydrogenase.

Abstract: The invention relates to preferably recombinant DNA derived from Corynebacterium and replicable in coryneform microorganisms, which contains at least one nucleotide sequence that codes for the thrE gene, and a process for the production of L-threonine.