Brevibacterium Or Corynebacterium Patents (Class 435/252.32)
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Publication number: 20100323409Abstract: A method for manufacturing (2S,3R,4S)-4-hydroxy-L-isoleucine or a salt thereof using an L-isoleucine-producing bacterium transformed with a DNA fragment containing a gene coding for a protein having L-isoleucine dioxygenase activity; and having the ability to produce (2S,3R,4S)-4-hydroxy-L-isoleucine.Type: ApplicationFiled: June 21, 2010Publication date: December 23, 2010Inventors: Sergey Vasilievich Smirnov, Natalia Nikolaevna Samsonova, Veronika Aleksandrovna Kotliarova, Natalia Yurievna Rushkevich, Olga Sergeevna Beznoschenko
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Publication number: 20100324258Abstract: The present invention relates to a novel method for the fermentative production of gamma-aminobutyric acid (GABA) by cultivating a recombinant micro-organism expressing an enzyme having a glutamate decarboxylase activity. The present invention also relates to corresponding recombinant hosts, recombinant vectors, expression cassettes and nucleic acids suitable for preparing such hosts as well as to a method for preparing polyamides making use of GABA as obtained fermentative production.Type: ApplicationFiled: February 20, 2009Publication date: December 23, 2010Applicant: BASF SEInventors: Oskar Zelder, Weol Kyu Jeong, Corinna Klopprogge, Andrea Herold, Hartwig Schröder
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Publication number: 20100324257Abstract: The present invention relates to a cell, which has been genetically modified relative to its wild type, so that in comparison with its wild type it is able to produce more ?-aminocarboxylic acids, more ?-aminocarboxylic acid esters or more lactams derived from ?-aminocarboxylic acids, starting from carboxylic acids or carboxylic acid esters. Furthermore, the present invention relates to a method for the production of a genetically modified cell, the cells obtainable by this method, a method for the production of ?-aminocarboxylic acids, of ?-aminocarboxylic acid esters or of lactams derived from ?-aminocarboxylic acids, the ?-aminocarboxylic acids, ?-aminocarboxylic acid esters or lactams derived from ?-aminocarboxylic acids obtainable by this method, a method for the production of polyamides based on ?-aminocarboxylic acids or based on lactams and the polyamides obtainable by this method.Type: ApplicationFiled: December 12, 2008Publication date: December 23, 2010Applicant: EVONIK DEGUSSA GmbhInventors: Andreas Karau, Volker Sieber, Thomas Haas, Harald Haeger, Katrin Grammann, Bruno Buehler, Lars Blank, Andreas Schmid, Guido Jach, Bernd Lalla, Andreas Mueller, Katrin Schullehner, Peter Welters, Thorsten Eggert, Andrea Weckbecker
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Publication number: 20100317067Abstract: Disclosed are a nucleic acid molecule of Corynebacterium glutamicum origin, having an improved promoter activity, which is operably linked to operon encoding aspartate kinase and aspartate semialdehyde dehydrogense, a vector containing the same, a transformant transformed with the vector, and a method for the production of L-lysine using the transformant.Type: ApplicationFiled: January 23, 2009Publication date: December 16, 2010Applicant: CJ CHEILJEDANG CORPORATIONInventors: Chul Ha Kim, Jong Soo Choi, Sang Jo Lim, Hyung Joon Kim, Jun Ok Moon, Gey Hang Jeon, Jin Suk Sung
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Patent number: 7851198Abstract: Disclosed is a novel L-lysine-inducible promoter nucleic acid molecule. Also disclosed are a vector containing the nucleic acid molecule, a host cell transformed with the vector, and a method of inducing expression of a target gene using the L-lysine-inducible promoter nucleic acid molecule.Type: GrantFiled: December 30, 2005Date of Patent: December 14, 2010Assignee: CJ Cheiljedang CorporationInventors: Young Hoon Park, Hyun Min Koo, Jun Ok Moon, Seong Jun Kim, Hyo Jin Kim, Jung Kee Lee
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Patent number: 7838278Abstract: Nucleotide and protein sequences that encode enzymes that change carbon flux through metabolic pathways that lead to lactic acid or fumarate production in a host cell, such as a R. oryzae cell, are provided. Methods of manipulating carbon flux in a cell also are provided.Type: GrantFiled: March 23, 2009Date of Patent: November 23, 2010Assignee: Archer-Daniels-Midland CompanyInventors: Beth Fatland-Bloom, P. John Rayapati, Nyerhovwo John Tonukari
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Publication number: 20100285546Abstract: Genetically modified microorganisms that produce itaconic acid at high yields and uses thereof.Type: ApplicationFiled: May 11, 2009Publication date: November 11, 2010Applicant: Industrial Technology Research InstituteInventors: James C. Liao, Pei-Ching Chang
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Patent number: 7829316Abstract: Disclosed is a process for production of succinic acid, which comprises the step of reacting a bacterium which has been modified so as to increase the expression of a sucE1 gene or a product produced by any treatment of the bacterium with an organic raw material in a reaction solution containing a carbonate ion, a bicarbonate ion or carbon dioxide gas to thereby yield the desired succinic acid.Type: GrantFiled: April 17, 2008Date of Patent: November 9, 2010Assignee: Ajinomoto Co., Inc.Inventors: Chie Koseki, Keita Fukui, Jun Nakamura, Hiroyuki Kojima
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Publication number: 20100279369Abstract: This invention concerns a microorganism useful for the production of acetol from a simple carbon source, wherein said microorganism is characterized by: an improved activity of the biosynthesis pathway from dihydroxyacetone phosphate to acetol, and an attenuated activity of the glyceraldehyde 3-phosphate dehydrogenase This invention also concerns a method for producing acetol by fermentating a microorganism according to the invention.Type: ApplicationFiled: March 21, 2008Publication date: November 4, 2010Applicant: Metabolic ExplorerInventors: Philippe Soucaille, Francois Voelker, Rainer Figge
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Publication number: 20100273222Abstract: The present invention relates to a microorganism of Corynebacterium ssp. having enhanced expression of gene for encoding molybdenum cofactor biosynthesis enzyme A and a method for producing L-lysine using the same, which has effects on providing the production method of L-lysine using the Corynebacterium strain having enhanced productivity of L-lysine by intensifying expression of the moaA gene for encoding molybdnum cofactor biosynthesis enzyme A.Type: ApplicationFiled: December 20, 2007Publication date: October 28, 2010Applicant: CJ CHEILJEDANG CORP.Inventors: Jun-Ok Moon, Jae-Woo Jang, So-Yeon Rah, Sang-Jo Lim, Jong-Soo Choi, Young-Hoon Park, Hyung-Joon Kim
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Publication number: 20100261257Abstract: An isolated polynucleotide encodes a polypeptide comprising the amino acid sequence of SEQ ID NO: 2, with the L-aspartic acid at position 5 of the amino acid sequence replaced by another proteinogenic amino acid, and possesses citrate synthase activity. In addition, a vector comprises the polynucleotide and a bacterium comprises the vector. An isolated polynucleotide comprises a nucleotide sequence comprising, from position 1 to 39, the nucleotide sequence corresponding to position 1 to 39 of SEQ ID NO: 11, from position 40 to 105, a nucleotide sequence encoding the amino acid sequence of SEQ ID NO: 12, with each proteinogenic amino acid except L-aspartic acid being present at position 5. A method of producing an L-amino acids is also described.Type: ApplicationFiled: March 31, 2010Publication date: October 14, 2010Applicant: EVONIK DEGUSSA GmbhInventors: Brigitte BATHE, Wilfried Claes
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Publication number: 20100261256Abstract: Provided are a novel promoter nucleic acid molecule having a nucleotide sequence of SEQ ID NO: 1 or 2 derived from Corynebacterium glutamicum, a recombinant vector comprising the promoter, a host cell transformed with the vector and a method of expressing genes of interest using the host cell.Type: ApplicationFiled: January 15, 2008Publication date: October 14, 2010Applicant: CJ CHEILJEDANG CORP.Inventors: So-Yeon Rah, Jae-Woo Jang, Sun-Young Lee, Young-Hoon Park, Sang-Jo Lim
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Publication number: 20100261241Abstract: Embodiments of the present invention include methods for the production of four carbon alcohols, specifically n-butanol, by a consolidated bioprocessing approach for the conversion of cellulosic material to the desired end product. According to some embodiments, recombinant microbial host cells are provided, preferably S. cerevisiae, that are capable of converting cellulosic material to butanol and include butanol biosynthetic pathway genes and cellulase genes.Type: ApplicationFiled: October 27, 2008Publication date: October 14, 2010Inventors: Nikolai Khramtsov, Alexander Amerik, Bruce E. Taillon, Steven Henck
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Publication number: 20100255037Abstract: The present invention relates to novel sequences of H. contortus and the proteins encoded therein. This invention also relates to immunogenic compositions, methods for their preparation and the diagnostic, prophylactic or therapeutic use of these sequences and the proteins encoded therein.Type: ApplicationFiled: November 15, 2007Publication date: October 7, 2010Inventors: Jennifer Louise Sexton, Dadna Hartman, Ben Cocks
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Publication number: 20100255544Abstract: The invention relates to coryneform bacteria which, instead of the singular copy of an open reading frame (ORF), gene or allele naturally present at the particular desired site (locus), have at least two copies of the open reading frame (ORF), gene or allele in question, preferably in tandem arrangement, and optionally at least a third copy of the open reading frame (ORF), gene or allele in question at a further gene site, and processes for the preparation of chemical compounds by fermentation of these bacteria.Type: ApplicationFiled: September 3, 2009Publication date: October 7, 2010Applicant: Evonik Degussa GmbHInventors: Brigitte Bathe, Caroline Kreutzer, Bettina Mockel, Georg Thierbach
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Patent number: 7807808Abstract: The invention relates to bacteria that have increased levels of protein secretion due to genetic modification, to nucleotide sequences and gene structures containing at least one gene coding for a SecA protein having increased levels of protein secretion, to a SecA having increased levels of protein secretion, and to a method for producing desired proteins using the inventive bacteria. The invention also relates to nucleic acids coding for a SecA protein having increased levels of protein secretion, containing a SecA gene sequence or allele, a SecA homologue or derivative, or nucleotide sequences hybridising therewith and comprising at least one mutation. Surprisingly, just one mutation in a nucleotide of a SecA gene leads to increased levels of protein secretion or to protein secretion for the first time.Type: GrantFiled: November 9, 2009Date of Patent: October 5, 2010Assignee: Danisco US Inc.Inventors: Oliver Koberling, Roland Freudl
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Publication number: 20100242345Abstract: Genetically engineered microorganisms are provided that produce products from the fatty acid biosynthetic pathway (fatty acid derivatives), as well as methods of their use.Type: ApplicationFiled: May 18, 2007Publication date: September 30, 2010Applicant: LS9, IncInventors: Jay D. Keasling, Zhihao Hu, Chris Sommerville, George Church, David Berry, Lisa Friedman, Andreas Schirmer, Shane Brubaker, Stephen B. del Cardayre
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Publication number: 20100248315Abstract: The present invention relates to newly identified microorganisms capable of direct production of L-ascorbic acid (hereinafter also referred to as Vitamin C). The invention also relates to polynucleotide sequences comprising genes that encode proteins which are involved in the synthesis of Vitamin C. The invention also features polynucleotides comprising the full length polynucleotide sequences of the novel genes and fragments thereof, the novel polypeptides encoded by the polynucleotides and fragments thereof, as well as their functional equivalents. The present invention also relates to the use of said polynucleotides and polypeptides as biotechnological tools in the production of Vitamin C from microorganisms, whereby a modification of said polynucleotides and/or encoded polypeptides has a direct or indirect impact on yield, production, and/or efficiency of production of the fermentation product in said microorganism.Type: ApplicationFiled: August 1, 2007Publication date: September 30, 2010Inventors: Marie-Gabrielle Beuzelin-Ollivier, Bastian Chevreux, Manuela Dalluegge, Marina Van Gelder, Markus G. Goese, Corina Hauk, Bertus P. Koekman, Connie Lee, Anne F. Mayer, Anja Meury, Nigel J. Mouncey, Dick Schipper, Masako Shinjoh, Christine Toepfer, Adrianus W.H. Vollebregt
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Publication number: 20100248306Abstract: The invention relates to an expression system for the production of one or more target polypeptide/target polypeptides, comprising a host cell in whose genome the DNA sequence that codes glycerine-3-phosphate dehydrogenase is inactivated or partially or completely deleted and which is transformed by an extrachromosomal element that comprises a DNA sequence that codes the target polypeptide(s) and glycerine-3-phosphate dehydrogenase, whereby not only the host cell genome but also the extrachromosomal element do not carry an antibiotic-resistance gene, as well as a DNA sequence that codes for a polypeptide with glycerine-3-phosphate dehydrogenase activity characterized in that the DNA sequence is selected from a) DNA sequences that comprise a nucleotide sequence according to SEQ ID NO: 1, b) DNA sequences that comprise a nucleotide sequence represented by the nucleotides 1338 to 2375 of SEQ ID NO: 1, c) DNA sequences that are coded by the plasmid pTP01 with the plasmid map according to FIG.Type: ApplicationFiled: March 12, 2008Publication date: September 30, 2010Applicant: AB Enzymes GmbHInventors: Celine Cadot, Tina Ploss, Ruth Schwerdtfeger, Bruno Winter
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Publication number: 20100248311Abstract: Process for the production of methionine or its derivatives by culturing a microorganism in an appropriate culture medium comprising a source of carbon and a source of sulfur. The microorganism and/or the culture medium are modified in such way that the methionine/carbon source yield is increased. The isolation of methionine or its derivates from the fermentation medium is also described.Type: ApplicationFiled: September 25, 2008Publication date: September 30, 2010Applicant: METABOLIC EXPLORERInventor: Rainer FIGGE
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Patent number: 7794990Abstract: Provided are a microorganism of Corynebacterium genus that has an inactivated endogenous NCgl1835 gene therein and produces L-lysine, and a method of producing L-lysine using the same.Type: GrantFiled: November 29, 2006Date of Patent: September 14, 2010Assignee: CJ Cheiljedang CorporationInventors: Young Hoon Park, Hyun Min Koo, Sang Jo Lim, Jun Ok Moon, So Yeon Rah, Young Lyeol Yang
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Publication number: 20100226891Abstract: The present invention relates to a fusion protein in which a myostatin mature protein is fused to a multimer of myostatin-derived antigenic peptide Myo-2, a surface expression vector containing a polynucleotide encoding the fusion protein, a recombinant microorganism transformed with the vector, and a feedstuff additive or a pharmaceutical composition containing the microorganism as an effective ingredient. The feedstuff additive or pharmaceutical composition according to the present invention can be used for muscle development and regulation of muscle growth in livestock and poultry, as well as for preventing and treating muscle-wasting diseases and degenerative diseases such as muscular dystrophy, muscular atrophy and the like. In addition, the transformed strain shows the same effect even if the strain itself after culture thereof is directly used, and thus it is very economical.Type: ApplicationFiled: November 30, 2007Publication date: September 9, 2010Applicant: BIOLEADERS CORPORATIONInventors: Moon-Hee Sung, Chul Joong Kim, Haryoung Poo, Ji Yeon Kim, Young Suk Kim, Long Chun Xu
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Patent number: 7785845Abstract: L-Glutamic acid is produced by culturing a microorganism in which an expression of L-glutamic acid-export gene, a yhfK gene, is enhanced or overexpressed, in a medium to produce and cause accumulation of L-glutamic acid in the medium, and collecting L-glutamic acid from the medium.Type: GrantFiled: August 24, 2007Date of Patent: August 31, 2010Assignee: Ajinomoto Co., Inc.Inventors: Yoshihiko Hara, Hiroshi Izui, Hisao Ito
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Patent number: 7785858Abstract: The present invention provides a bacterium which has an ability to produce a useful metabolite derived from acetyl-coenzyme A, such as L-glutamic acid, L-glutamine, L-proline, L-arginine, L-leucine, L-cysteine, succinate, and polyhydroxybutyrate, wherein said bacterium is modified so that activities of D-xylulose-5-phosphate phosphoketolase and/or fructose-6-phosphate phosphoketolase are enhanced. The present invention also provides a method for producing the useful metabolite using the bacterium.Type: GrantFiled: August 10, 2005Date of Patent: August 31, 2010Assignee: Ajinomoto Co., Inc.Inventors: Yury Ivanovich Kozlov, Akito Chinen, Hiroshi Izui, Yoshihiko Hara, Hisashi Yasueda, Konstantin Vyacheslavovich Rybak, Ekaterina Aleksandrovna Slivinskaya, Joanna Yosifovna Katashkina
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Patent number: 7785840Abstract: An isolated polynucleotide encodes a polypeptide comprising the amino acid sequence of SEQ ID NO: 2, with the L-aspartic acid at position 5 of the amino acid sequence replaced by another proteinogenic amino acid, and possesses citrate synthase activity. In addition, a vector comprises the polynucleotide and a bacterium comprises the vector. An isolated polynucleotide comprises a nucleotide sequence comprising, from position 1 to 39, the nucleotide sequence corresponding to position 1 to 39 of SEQ ID NO: 11, from position 40 to 105, a nucleotide sequence encoding the amino acid sequence of SEQ ID NO: 12, with each proteinogenic amino acid except L-aspartic acid being present at position 5. A method of producing an L-amino acids is also described.Type: GrantFiled: July 13, 2007Date of Patent: August 31, 2010Assignee: Evonik Degussa GmbHInventors: Brigitte Bathe, Wilfried Claes
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Publication number: 20100209986Abstract: Provided herein are metabolically-modified microorganisms useful for producing biofuels. More specifically, provided herein are methods of producing high alcohols including isobutanol, 1-butanol, 1-propanol, 2-methyl-1-butanol, 3-methyl-1-butanol and 2-phenylethanol from a suitable substrate.Type: ApplicationFiled: August 12, 2009Publication date: August 19, 2010Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIAInventors: James C. Liao, Shota Atsumi, Anthony F. Cann
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Publication number: 20100203599Abstract: Provided are mutant microorganisms having the ability to produce a high concentration of putrescine wherein gene(s) involved in the putrescine degradation or utilization pathway is inactivated or deleted and a preparation method thereof. A method for producing putrescine in high yield by culturing the mutant microorganisms is also provided. The mutant microorganisms are useful for producing a high concentration of putrescine which can be widely used in various industrial applications.Type: ApplicationFiled: October 14, 2009Publication date: August 12, 2010Applicant: KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGYInventors: Sang Yup Lee, Zhi Gang Qian, Xiaoxia Xia, Yong Jae Jeon
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Publication number: 20100190653Abstract: The invention relates to an isolated polynucleotide having a polynucleotide sequence which codes for the alr gene, and a host-vector system having a coryneform host bacterium in which the alr gene is present in attenuated form and a vector which carries at least the alr gene according to SEQ ID No 1, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.Type: ApplicationFiled: November 27, 2002Publication date: July 29, 2010Inventors: Andreas Tauch, Michael Binder, Walter Pfefferle, Georg Thierbach, Jorn Kalinowski, Alfred Puhler
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Patent number: 7763447Abstract: Succinic acid is produced by allowing a bacterium modified to enhance fumarate reductase activity or cell preparation thereof to react with an organic raw material in a reaction solution containing one of a carbonate ion, a bicarbonate ion, and carbon dioxide gas to generate succinic acid. More preferably, succinic acid is produced by allowing a bacterium modified to enhance activities of fumarate reductase and pyruvate carboxylase and decrease lactate dehydrogenase activity or cell preparation thereof to react with an organic raw material in a reaction solution containing one of a carbonate ion, a bicarbonate ion, and carbon dioxide gas to generate succinic acid. Succinic acid is obtained by collecting the produced succinic acid.Type: GrantFiled: February 28, 2006Date of Patent: July 27, 2010Assignee: Ajinomoto Co., Inc.Inventors: Makoto Murase, Ryusuke Aoyama, Miki Ikuta, Kenji Yamagishi, Mika Moriya, Jun Nakamura, Hiroyuki Kojima
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Publication number: 20100185017Abstract: Methods, enzymes, recombinant microorganism, and microbial systems are provided for converting suitable monosaccharides or oligosaccharides, such as those derived from biomass, as well as various aldehydes and/or ketones, into commodity chemicals, such as biofuels. Commodity chemicals produced by the methods described herein are also provided. Commodity chemical enriched, refinery-produced petroleum products are also provided, as well as methods for producing the same.Type: ApplicationFiled: December 11, 2009Publication date: July 22, 2010Inventors: Yasuo Yoshikuni, Adam J. Wargacki, Asael Herman
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Publication number: 20100184171Abstract: Genetically engineered microorganisms have been constructed to produce succinate and malate in mineral salt media in pH-controlled batch fermentations without the addition of plasmids or foreign genes. The subject invention also provides methods of producing succinate and malate comprising the culture of genetically modified microorganisms.Type: ApplicationFiled: March 19, 2008Publication date: July 22, 2010Inventors: Kaemwich Jantama, Mark John Haupt, Xueli Zhang, Jonathan C. Moore, Keelnatham T. Shanmugam, Lonnie O'Neal Ingram
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Publication number: 20100184163Abstract: A polypeptide comprising an amino acid sequence in which one or more amino acids are deleted, substituted or added in the amino acid sequence of a glutamine synthetase 2 derived from a microorganism belonging to a coryneform bacterium, wherein the coryneform bacterium producing the polypeptide has L-glutamine productivity, a DNA encoding the polypeptide, a recombinant DNA comprising the DNA, a microorganism comprising the DNA or the recombinant DNA, and a process for producing L-glutamine using the microorganism are provided.Type: ApplicationFiled: December 27, 2006Publication date: July 22, 2010Applicant: KYOWA HAKKO KOGYO CO., LTD.Inventors: Mikiro Hayashi, Masaki Maeda, Yoshiyuki Yonetani
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Publication number: 20100184142Abstract: The invention relates to bacteria that have increased levels of protein secretion due to genetic modification, to nucleotide sequences and gene structures containing at least one gene coding for a SecA protein having increased levels of protein secretion, to a SecA having increased levels of protein secretion, and to a method for producing desired proteins using the inventive bacteria. The invention also relates to nucleic acids coding for a SecA protein having increased levels of protein secretion and containing a gene sequence SecA or an allele, homologue or derivative of said nucleotide sequences or nucleotide sequences hybridising therewith and comprising at least one mutation. Surprisingly, just one mutation in a nucleotide of a SecA gene leads to increased levels of protein secretion or to protein secretion for the first time.Type: ApplicationFiled: November 9, 2009Publication date: July 22, 2010Inventors: Oliver Koberling, Roland Freudl
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Publication number: 20100172976Abstract: A therapeutic composition for inhibiting the function of a target polynucleotide sequence in a mammalian cell includes an agent that provides to a mammalian cell an at least partially double-stranded RNA molecule comprising a polynucleotide sequence of at least about 200 nucleotides in length, said polynucleotide sequence being substantially homologous to a target polynucleotide sequence. This RNA molecule desirably does not produce a functional protein. The agents useful in the composition can be RNA molecules made by enzymatic synthetic methods or chemical synthetic methods in vitro; or made in recombinant cultures of microorganisms and isolated therefrom, or alternatively, can be capable of generating the desired RNA molecule in vivo after delivery to the mammalian cell.Type: ApplicationFiled: December 18, 2009Publication date: July 8, 2010Applicant: ALNYLAM PHARMACEUTICALS, INC.Inventors: Chandrasekhar Satishchandran, Catherine J. Pachuk
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Publication number: 20100173356Abstract: The present invention relates to newly identified mRNA stabilizing elements useful for the production of a target fermentation product, such as e.g. vitamins or enzymes, in particular riboflavin (vitamin B2), biotin, pantothenic acid (vitamin B5), folic acid, thiamin, pyridoxine (vitamin B6), vitamin B12, xylanase, amylase, protease, glucanase, amylomaltase or maltogenic amylase.Type: ApplicationFiled: June 9, 2008Publication date: July 8, 2010Inventors: Martin Lehmann, Zoltan Pragai, Michèle Schaber
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Patent number: 7741081Abstract: The present invention provides a polypeptide which has: (i) an amino acid sequence wherein one or more amino acid residues are substituted in the region at positions 20 to 38 from the N terminus of the amino acid sequence shown in SEQ ID NO: 1; or (ii) an amino acid sequence wherein one or more amino acid residues are substituted in the region at positions 20 to 38 from the N terminus of the amino acid sequence shown in SEQ ID NO: 1 and one or more amino acid residues are deleted, substituted or added in the region at positions 1 to 19 or 39 to 294; and which has N-acetylglutamate kinase activity.Type: GrantFiled: September 28, 2005Date of Patent: June 22, 2010Assignee: Kyowa Hakko Bio Co., Ltd.Inventors: Masato Ikeda, Tetsuo Nakano, Satoshi Mitsuhashi, Mikiro Hayashi, Kenji Tanaka
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Publication number: 20100151449Abstract: The invention relates to a method for production of L-amino acids by fermentation. According to the invention, the activity of the alanine transaminase is ether reduced or inhibited, whereby in particular the amino acids L-valine, L-lysine and L-isoleucine are produced with increased yield. Furthermore, the nucleic acids according to seq. No. 1 from position 101 to 1414 are identified as the sequence coding for the alanine transaminase gene. Use of the above permits the production of L-alanine.Type: ApplicationFiled: April 20, 2006Publication date: June 17, 2010Inventors: Jan Marinhagen, Lothar Eggeling, Hermann Sahm
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Publication number: 20100143989Abstract: This invention relates to nitrilase mutants having improved nitrilase activity for converting 3-hydroxynitriles to 3-hydroxycarboxylic acids. More specifically, the Acidavorax facilis 72W (ATCC 55746) nitrilase gene was mutated using error-prone PCR and site-directed mutagenesis to create nitrilase enzymes having improved nitrilase activity for converting 3-hydroxynitriles (e.g., 3-hydroxybutyronitrile or 3-hydroxyvaleronitrile) to the corresponding 3-hydroxycarboxylic acids. A process using these improved mutants to produce the 3-hydroxycarboxylic acids is also provided.Type: ApplicationFiled: April 14, 2008Publication date: June 10, 2010Applicant: E. I. DU PONT DE NEMOURS AND COMPANYInventors: Mark S. Payne, Robert DiCosimo, Daniel P. O'Keefe
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Publication number: 20100143984Abstract: A variant of Corynebacterium shows activity greater than the endogenous activity of aspartate aminotransferase, aspartate kinase, aspartate semialdehyde dehydrogenase, dihydrodipicolinate synthase, dihydropicolinate reductase and diaminopimelate dicarboxylase and additionally pyruvate carboxylase. The variant is used in a method of producing L-lysine.Type: ApplicationFiled: September 17, 2007Publication date: June 10, 2010Applicant: CJ CHEILJEDANG CORPORATIONInventors: Young Hoon Park, Sang Jo Lim, Jun Ok Moon, So Yeon Rah, Hee Jong Lee, Jae Woo Jang, Do Hyun Kwon, Hyo Jin Kim, Jin Suck Sung, Hyung Joon Kim
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Publication number: 20100136621Abstract: Peptide tags, referred to here as inclusion body tags, are disclosed useful for the generation of insoluble fusion peptides. The fusion peptides comprise at least one inclusion body tag operably linked to a peptide of interest. Expression of the fusion peptide in a host cell results in a product that is insoluble and contained within inclusion bodies in the cell and/or cell lysate. The inclusion bodies may then be purified and the protein of interest may be isolated after cleavage from the inclusion body tag.Type: ApplicationFiled: January 25, 2010Publication date: June 3, 2010Applicant: E.I. DU PONT DE NEMOURS AND COMPANYInventors: Qiong Cheng, Linda Jane Decarolis, Stephen R. Fahnestock, Tanja Maria Gruber, Lisa Diane Reiss, Pierre E. Rouviere
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Publication number: 20100129885Abstract: Embodiments of the present invention include methods for the production of four carbon alcohols, specifically n-butanol, by a consolidated bioprocessing approach for the conversion of cellulosic material to the desired end product. According to some embodiments, recombinant microbial host cells are provided, preferably S. cerevisiae, that are capable of converting cellulosic material to butanol and include butanol biosynthetic pathway genes and cellulase genes. According to some embodiments, recombinant microbial host cells are provided, preferably S. cerevisiae, that are capable of converting hemicellulosic material to butanol and include cellulase genes, butanol biosynthetic pathway genes and at least one gene for the conversion of a pentose sugar.Type: ApplicationFiled: October 26, 2009Publication date: May 27, 2010Applicant: Arbor Fuel Inc.Inventors: Nikolai Khramtsov, Alexander Amerik, Bruce E. Taillon, Steven A. Henck
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Publication number: 20100129884Abstract: The present invention relates to a method for producing fermentation product from various carbon sources containing glycerol using Corynebacteria. More particularly, the present invention relates to a method for producing fermentation product from carbon sources containing glycerol or a part of glycerol with high yield and high productivity, by fermenting Corynebacteria introduced with the foreign gene glpDFK facilitating the use of glycerol and accumulating industrially useful amino acids in the culture medium.Type: ApplicationFiled: January 22, 2008Publication date: May 27, 2010Applicant: CJ CHEILJEDANG CORPORATIONInventors: Kwang-myung Cho, Kyung-oh Choi, Hyun-ae Bae
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Publication number: 20100120126Abstract: Microorganisms in which the activities of one or more kinds of peptidases and one or more kinds of proteins having peptide-transporting activity are reduced or lost and which have the ability to produce a dipeptide. Also, microorganisms in which the activities of three or more kinds of peptidases are reduced or lost and which have the ability to produce a dipeptide, and a process for producing dipeptides using the microorganisms.Type: ApplicationFiled: November 10, 2009Publication date: May 13, 2010Applicant: KYOWA HAKKO BIO CO., LTD.Inventors: Shin-ichi Hashimoto, Kazuhiko Tabata
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Publication number: 20100112656Abstract: The invention provides recombinant bacteria, which comprise a full complement of heterologous ethanol production genes. Expression of the full complement of heterologous ethanol production genes causes the recombinant bacteria to produce ethanol as the primary fermentation product when grown in mineral salts medium, without the addition of complex nutrients. Methods for producing the recombinant bacteria and methods for producing ethanol using the recombinant bacteria are also disclosed.Type: ApplicationFiled: August 8, 2007Publication date: May 6, 2010Applicant: UNIVERSITY OF FLORIDA RESEARCH FOUNDATION, INC.Inventors: Lorraine P. Yomano, Sean W. York, Shengde Zhou, Keelnatham Shanmugam, Lonnie O. Ingram
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Publication number: 20100112651Abstract: Nucleotide and protein sequences that encode enzymes that change carbon flux through metabolic pathways that lead to lactic acid or fumarate production in a host cell, such as a R. oryzae cell, are provided. Methods of manipulating carbon flux in a cell also are provided.Type: ApplicationFiled: March 23, 2009Publication date: May 6, 2010Applicant: Archer-Daniels-Midland CompanyInventors: Beth Fatland-Bloom, P. John Rayapati, Nyerhovwo John Tonukari
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Publication number: 20100068773Abstract: The present invention relates to a cell which has been modified in comparison with its wild type in such a way that it is capable of forming more, by comparison with its wild, 3-hydroxyisobutyric acid or poly-hydroxyalkanoates based on 3-hydroxyisobutyric acid via methylmalonate-semialdehyde or 3-hydroxybutyryl-coenzyme A as precursors. The invention also relates to a method of generating a genetically modified cell, to the genetically modified cell obtainable by these methods, to a method of producing 3-hydroxyisobutyric acid or polyhydroxyalkanoates based on 3-hydroxyisobutyric acid, to a method of producing methacrylic acid or methacrylic esters, and to a method of producing polymethacrylic acid or polymethacrylic esters. The present invention furthermore relates to an isolated DNA, to a vector, to the use of this vector for transforming a cell, to a transformed cell, and to a polypeptide.Type: ApplicationFiled: June 1, 2007Publication date: March 18, 2010Applicant: Evonik Roehm GmbHInventors: Achim Marx, Markus Poetter, Stefen Buchholz, Alexander May, Hermann Siegert, Georg Fuchs, Birgit Alber, Lothar Eggeling
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Publication number: 20100068769Abstract: A coryneform bacterium that is modified by using a yggB gene so that L-glutamic acid-producing ability is enhanced as compared to a non-modified strains is cultured in a medium to cause accumulation of L-glutamic acid in the medium or bacterial cells, and L-glutamic acid is collected from the medium or cells.Type: ApplicationFiled: August 31, 2009Publication date: March 18, 2010Inventors: Jun Nakamura, Seiko Hirano, Hisao Ito
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Publication number: 20100041107Abstract: The present invention is directed to a method of reducing the amount of at least one polypeptide in a host cell by expressing a nucleotide sequence encoding for the polypeptide in the host cell wherein the nucleotide sequence uses codons that are rarely used according to the codon usage of the host organism. Furthermore, the present invention relates to nucleotide sequences encoding for a polypeptide with a codon usage that has been adjusted to use codons that are only rarely used according to the codon usage of the host organism. The present invention further relates to the use of such sequences and methods for producing fine chemicals such as amino acids, sugars, lipids, oils, carbohydrates, vitamins, cofactors etc.Type: ApplicationFiled: October 18, 2007Publication date: February 18, 2010Applicant: BASF SEInventors: Andrea Herold, Corinna Klopprogge, Hartwig Schröder, Osker Zelder, Weol Kyu Jeong
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Publication number: 20100028957Abstract: The present invention relates to a microorganism of Corynebacterium genus having enhanced L-lysine productivity and a method of producing L-lysine using the same. More particularly, the present invention relates to a recombinant microorganism of Corynebacterium genus having enhanced L-lysine productivity by inactivating endogenous NCgI 1090 gene having the amino acid sequence containing repeated aspartate residues and a method of producing L-lysine using the same.Type: ApplicationFiled: December 28, 2007Publication date: February 4, 2010Applicant: CJ CHEILJEDANG CORPORATIONInventors: Hyun-min Koo, Young-lyeol Yang, Hyo-jin Kim, Jun-ok Moon, Sang-jo Lim, Jong-soo Choi, Young-hoon Park
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Publication number: 20100015673Abstract: The present invention relates to a microorganism of Corynebacterium genus having enhanced L-lysine productivity and a method of producing L-lysine using the same. More particularly, the present invention relates to a recombinant microorganism of Corynebacterium genus having enhanced L-lysine productivity by inactivating endogenous NCgl2534 gene having the amino acid sequence containing repeated lysine residues and a method of producing L-lysine using the same.Type: ApplicationFiled: December 28, 2007Publication date: January 21, 2010Applicant: CJ CHEILJEDANG CORPORATIONInventors: Hyun-min Koo, Sun-young Lee, Young-lyeol Yang, Hyo-jin Kim, Jun-ok Moon, Jae-woo Jang, Sang-jo Lim, Jong-soo Choi, Young-hoon Park