Streptomyces Patents (Class 435/252.35)
-
Publication number: 20140223596Abstract: The present invention provides use of a plant gene JAZ5a for improving drought-resistance of a plant. It further provides a method for improving the drought-resistance of a plant, comprising enhancing the expression or activity of Jaz5A in said plant.Type: ApplicationFiled: July 5, 2012Publication date: August 7, 2014Inventor: Adrianus Johannes Van Tunen
-
Publication number: 20140206594Abstract: The present invention relates to isolated polypeptides having protease activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: June 22, 2012Publication date: July 24, 2014Inventors: Martin Simon Borchert, Jeppe Wegener Tams
-
Publication number: 20140205601Abstract: The present invention relates to amino acid sequences that are directed against RANK-L, as well as to compounds or constructs, and in particular proteins and polypeptides, that comprise or essentially consist of one or more such amino acid sequences. The invention also relates to nucleic acids encoding such amino acid sequences and polypeptides; to methods for preparing such amino acid sequences and polypeptides; to host cells expressing or capable of expressing such amino acid sequences or polypeptides; to compositions, and in particular to pharmaceutical compositions, that comprise such amino acid sequences, polypeptides, nucleic acids and/or host cells; and to uses of such amino acid sequences or polypeptides, nucleic acids, host cells and/or compositions, in particular for prophylactic, therapeutic or diagnostic purposes.Type: ApplicationFiled: December 11, 2013Publication date: July 24, 2014Applicant: Ablynx N.V.Inventors: Els Anna Alice Beirnaert, Sigrid Cornelis, Hendricus Renerus Jacobus Mattheus Hoogenboom
-
Publication number: 20140206068Abstract: A process for the fermentative preparation of L-ornithine using microorganisms characterized by an increased export of the amino acid.Type: ApplicationFiled: February 20, 2014Publication date: July 24, 2014Applicant: Evonik Degussa GmbHInventors: Wilfried CLAES, Robert Gerstmeir
-
Publication number: 20140206035Abstract: Provided are isolated polypeptides having glucoamylase activity, catalytic domains, and polynucleotides encoding the polypeptides, catalytic domains. Also provided are nucleic acid constructs, vectors and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains.Type: ApplicationFiled: August 24, 2012Publication date: July 24, 2014Inventors: Tianqi Sun, Ming Li
-
Publication number: 20140206045Abstract: The present invention relates to an isolated polynucleotide comprising an open reading frame encoding a polypeptide having alpha-amylase activity, the polypeptide selected from the group consisting of: a) a polypeptide comprising an amino acid sequence which has at least 70% identity with amino acids 22 to 450 of SEQ ID NO: 4; b) a polypeptide comprising an amino acid sequence which has at least 70% identity with the polypeptide encoded by the amylase encoding part of the polynucleotide inserted into a plasmid present in the E. coli host deposited under the Budapest Treaty with DSMZ under accession number DSM 15334; c) a polypeptide encoded by a polynucleotide comprising a nucleotide sequence which has at least 70% identity with the sequence shown from position 68 to 1417 in SEQ ID NO: 3; and d) a fragment of (a), (b) or (c) that has alpha-amylase activity.Type: ApplicationFiled: March 31, 2014Publication date: July 24, 2014Applicant: Novozymes A/SInventors: Lan Tang, Wenping Wu, Junxin Duan, Pia Francke Johannesen
-
Publication number: 20140201871Abstract: The present invention relates to isolated polypeptides having beta-glucosidase activity, beta-xylosidase activity, or beta-glucosidase and beta-xylosidase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: March 25, 2014Publication date: July 17, 2014Applicant: Novozymes Inc.Inventor: Marc Dominique Morant
-
Publication number: 20140199293Abstract: Compositions and methods for making and using humanized anti-LPA monoclonal antibodies, and fragments and derivatives thereof, are described.Type: ApplicationFiled: November 19, 2013Publication date: July 17, 2014Inventors: Roger A. SABBADINI, Genevieve HANSEN, William A. GARLAND, Steven Tarran JONES, David Gareth WILLIAMS
-
Publication number: 20140193879Abstract: The present invention relates to isolated polypeptides having organophosphorous hydrolase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: March 13, 2014Publication date: July 10, 2014Applicant: Novozymes A/SInventors: Steffen Danielsen, Lars Kobberoee Skov, Ricardo Leite, Vincent Laize, M. Leonor Cancela Da Fonseca
-
Publication number: 20140193431Abstract: Disclosed are a human antibody comprising a human complementarity-determining region (CDR), which binds specifically to c-Met, and a framework region (FR), a polynucleotide encoding the human antibody, an expression vector comprising the polynucleotide, a transformant transformed with the expression vector, a method of producing the human antibody B7 by culturing the transformant, a wound healing composition comprising the human antibody as an active ingredient, a cell regeneration composition comprising the antibody as an active ingredient, and a drug conjugate comprising a drug linked to the human antibody. The c-Met-specific human antibody can function as an HGF mimic that can be used as a wound healing composition. The antibody can be widely used to determine the treatment and prognosis of various diseases, including neuronal infarction, progressive nephropathy, liver cirrhosis, lung fibrosis, kidney injury, liver injury, lung injury, and ulcerative wounds, which are treated by activation of HGF or c-Met.Type: ApplicationFiled: June 4, 2012Publication date: July 10, 2014Applicant: Korea Research Institute of Bioscience and BiotechnologyInventors: Young Woo Park, KiWon Jo, Chan Woong Park, Seok Ho Yoo, Myeoung Hee Jang, Hye Nan Kim, Seon Ha Yun, Kyu Won Cho, Mi Ra Park
-
Publication number: 20140193417Abstract: The present disclosure relates generally to the membrane transporter NaPi2b (SLC34A2) as a target for therapy, including immunotherapy, and particularly cancer therapy. The SLC34A2 epitope peptide encompassing amino acids 312-340 of SLC34A2 has been identified as an ovarian cancer epitope using the monoclonal antibody MX35. The invention also relates to the use of SLC34A2 and particularly SLC34A2 peptides in generating antibodies which have anti-tumor or anti-cancer activity or in stimulating an immunological response. The invention further relates to antibodies specifically directed against NaPi2b (SLC34A2) and the SLC34A2 peptide(s), including veneered, chimeric, single chain and humanized antibodies. Methods for generating an immune response and for treatment of tumors and cancer are also provided. Assays for screening and identifying compounds directed against SLC34A2, including the SLC34A2 epitope peptide, and additional antibodies are provided.Type: ApplicationFiled: September 26, 2013Publication date: July 10, 2014Inventors: Gerd Ritter, Beatrice Yin, Anne Murray, George Mark, Lloyd J. Old, Kenneth Lloyd, Serhiy Souchelnytskiy, Ivan Gout, Valeriy Filonenko, Ramziya Kiyamova
-
Publication number: 20140186887Abstract: Isolated receptors, DNAs encoding such receptors, and pharmaceutical compositions made therefrom, are disclosed. The isolated receptors can be used to regulate an immune response. The receptors are also useful in screening for inhibitors thereof.Type: ApplicationFiled: October 28, 2013Publication date: July 3, 2014Applicant: IMMUNEX CORPORATIONInventor: DIRK M. ANDERSON
-
Publication number: 20140178362Abstract: Methods and compositions for detecting and treating a disease, particularly cancer, associated with differential expression of Maba in disease cells compared to healthy cells. Also provided are antagonists or agonists of Maba, and methods for screening agents that modulate the Maba level or activity in vivo or in vitro.Type: ApplicationFiled: July 1, 2013Publication date: June 26, 2014Applicant: CELERA CORPORATIONInventors: Charles E. BIRSE, Bruno DOMON, Steve RUBEN, Candy LEE
-
Publication number: 20140179588Abstract: The present invention relates to isolated polypeptides having cellulase activity. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: August 10, 2012Publication date: June 26, 2014Applicant: Novozymes A/SInventors: Kirk Matthew Schnorr, Lars Anderson, Maria Leonor Quintais Cancela Da Fonseca, Ricardo Leite
-
Publication number: 20140178391Abstract: The present invention provides a method for diagnosing and detecting diseases associated with colon. The present invention provides one or more proteins or fragments thereof, peptides or nucleic acid molecules differentially expressed in colon diseases (CCAT) and antibodies binds to CCAT. The present invention provides that CCAT is used as targets for screening agents that modulates the CCAT activities. Further the present invention provides methods for treating diseases associated with colon.Type: ApplicationFiled: October 18, 2013Publication date: June 26, 2014Applicant: Celera CorporationInventors: Bruno DOMON, Aiqun LI, Tao HE, Ian MCCAFFREY
-
Patent number: 8759033Abstract: The present invention relates to genetically modified actinobacteria for the production of an enzyme having chitosanase activity. The genetically modified actinobacteria have a reduced (or abolished) activity of the CsnR polypeptide. Such reduced activity can be obtained by reducing the capacity of expressing the csnR gene, its corresponding transcript or expressing a dominant-negative CsnR polypeptide. Such genetically modified actinobacteria are less dependent (and, in some embodiment, totally independent) on the presence of chitosan in the culture medium for producing an enzyme having chitosanase activity. In addition, the genetically modified bacteria produce less proteases in the culture medium and ultimately provide a chitosanase end-product with higher purity.Type: GrantFiled: February 8, 2013Date of Patent: June 24, 2014Assignee: Socpra Sciences et Genie S.E.C.Inventors: Ryszard Brzezinski, Marie-Pierre Dubeau
-
Patent number: 8759032Abstract: A genetically engineered strain WSJ-IA for producing isovaleryl spiramycin I. Also provided is a method for preparing the strain, including the steps of: (a) constructing a recombinant plasmid including a double gene ist-acyB2; (b) transforming the plasmid into an isovaleryl spiramycin I-producing strain to obtain the strain WSJ-IA. The level of isovaleryl spiramycin I produced by fermentation of the strain WSJ-IA is increased 1.7 times and the fermentation potency thereof increased 4.14 times in comparison with the strain exclusively including a single gene ist.Type: GrantFiled: July 21, 2011Date of Patent: June 24, 2014Assignee: Shen Yang Tonglian Group Co., Ltd.Inventors: Yiguang Wang, Yang Jiang, Shengwu Yang, Xiaofeng Zhao
-
Publication number: 20140162936Abstract: The present invention relates to a polypeptide binding to a chymase (EC 3, 4, 21,39), wherein the polypeptide comprises or consists of an amino acid sequence selected from the group consisting of: (a) GVTLFVALYDY(X1)A(X2)(X3)(X4)(X5) (X6)LSFHKGEKFQIL(X7 (X8)(X9)(X10) (X11)(X12)G(X13)(X14)WEARSLTTGETGYIPSNYVAPVDSIQ (SEQ ID NO: 1), wherein (X1) is R, N, Q, E, K, H, S, T, C, or D; (X2) is E, T, D, Q, L, P, A, S, C, M, N, E, G, A, V or I; (X3) is R, T, H, N, K, S, C, N or Q; (X4) is S, W, T, C, N, Q, For Y; (X5) is T, H, L, F, C, S, M, N, Q, R, K, G, A, V, I, P, Y or W; (X6) is D, Q, H, E, S, T, C, N, R or K; (X7) is D, N, R, E, Q, S, T, C, K or D; (X8) is M, W, G, F, A, S, T, C, S, N, Q, Y, V, L, I or P; (X9) is T, H, S, D, C, N, Q, R, K, E or absent; (X10) is V, T, Q, G, A, L, I, P, S, C, M, N or absent; (X11) is P, A, D, G, K, V, L, I, E, R, M, H or absent; (X12) is N, V, P, I, E, T, S, A, G, L, C, M, Q or D; (X13) is D, E, T, P, G, A, V, L, I, S, C, M, N or Q, and (X14) is W, Y, L, G, A, V, I, P, M, or F; (b)Type: ApplicationFiled: April 24, 2012Publication date: June 12, 2014Applicant: COVAGEN AGInventors: Simon Brack, Sarah Batey, Dragan Grabulovski, Julian Bertschinger, Daniel Schlatter, Jörg Benz, David Banner, Michael Hennig
-
Publication number: 20140162331Abstract: Disclosed are an amphipathic peptide-lipase conjugate with enhanced lipase activity, a polynucleotide coding for the conjugate, an expression vector carrying the polynucleotide, a transformant anchoring the expression vector therein, a method for preparing the conjugate, a lipolysis method using the conjugate, and a method for producing biodiesel using the lipase.Type: ApplicationFiled: August 10, 2012Publication date: June 12, 2014Inventors: Sun-Chang Kim, Bong Hyun Sung, Kyung Seok Yang, Jun Hyoung Lee, Ki Jung Lim, Myung Keun Park
-
Publication number: 20140162322Abstract: The invention provides, inter alia, compositions and methods for the biological production of pentose sugars, such as 2-methylerythritol (2-ME), 1-deoxyxylulose (1-DX), and monoacetylated-2-C-methylerythritols, using recombinant cells.Type: ApplicationFiled: February 13, 2014Publication date: June 12, 2014Applicant: DANISCO US INC.Inventors: Joseph C. MCAULIFFE, Rachel E. MUIR
-
Publication number: 20140154753Abstract: Testosteronan, a heparosan analog having the structure [-4-D-GlcUA-?1,4-D-GlcNAc-?1-]n, is produced by testosteronan synthase, a single protein that is a dual-action catalyst that utilizes UDP-GlcUA and UDP-GlcNAc to synthesize a polysaccharide having the structure [-4-D-GlcUA-?1,4-D-GlcNAc-?1-]n.Type: ApplicationFiled: January 31, 2012Publication date: June 5, 2014Applicant: The Board of Regents of the University of OklahomaInventors: Paul L. DeAngelis, Nigel J. Otto
-
Publication number: 20140154252Abstract: Variations on the structural themes for multivalent binding molecules with effector function, or scorpions, will be apparent to those of skill in the art upon review of the present disclosure, and such variant structures are within the scope of the invention.Type: ApplicationFiled: March 15, 2013Publication date: June 5, 2014Applicant: EMERGENT PRODUCT DEVELOPMENT SEATTLE, LLCInventors: PETER ARMSTRONG THOMPSON, JEFFREY A. LEDBETTER, MARTHA SUSAN HAYDEN-LEDBETTER, WILLIAM BRADY
-
Publication number: 20140150137Abstract: The present invention relates to isolated polypeptides having endoglucanase activity, catalytic domains, cellulose binding domains and polynucleotides encoding the polypeptides, catalytic domains or cellulose binding domains. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains or cellulose binding domains.Type: ApplicationFiled: April 26, 2012Publication date: May 29, 2014Applicant: Novozymes, Inc.Inventors: Nikolaj Spodsberg, Brett McBrayer, Pierre Cassland
-
Publication number: 20140148355Abstract: The present invention relates to polypeptides that bind to H3K4 methylated chromatin, and in particular to the use of reagents comprising such polypeptides for epigenetic/epigenomic analysis.Type: ApplicationFiled: March 28, 2012Publication date: May 29, 2014Applicant: UNIVERSITY OF OSLOInventors: Reidunn B. Aalen, Tage Thorstensen, Rein Aasland, Verena Hoppmann
-
Publication number: 20140147458Abstract: The present invention relates to novel Varicella-zoster virus strain and to a chicken pox and herpes zoster virus vaccine using the same. More particularly, the present invention relates to genome DNA of VZV MAV/06 strain isolated from a Korean patient, to an open reading frame (ORF) thereof, to a protein coded by the genome DNA of VZV MAV/06 strain and the ORF thereof, and to a vaccine composition which contains the protein as an active ingredient.Type: ApplicationFiled: February 24, 2012Publication date: May 29, 2014Applicant: MOGAM BIOTECHNOLOGY RESEARCH INSTITUTEInventors: Geun Hee Kim, Shi Nae Kwon, Chan Hee Lee, Yeop Yoon
-
Publication number: 20140147893Abstract: The present invention relates to variants of alpha amylase. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.Type: ApplicationFiled: July 6, 2012Publication date: May 29, 2014Applicant: NOVOZYMES A/SInventors: Tomoko Matsui, Aki Tomiki, Guillermo Coward-Kelly
-
Patent number: 8735134Abstract: The present invention provides methods and compositions of variant polypeptides having isoprene synthase activity with improved solubility. In particular, the present invention provides isoprene synthase variant for increased isoprene production in recombinant host cells.Type: GrantFiled: October 26, 2012Date of Patent: May 27, 2014Assignees: Danisco US Inc., The Goodyear Tire & Rubber CompanyInventors: Christopher L. Rife, Derek H. Wells
-
Publication number: 20140141467Abstract: The present invention demonstrates the utility of carbonic acid amides such as urea or its derivatives, carbamates, carbodiimides & thiocarbamides as nitrogenous supplements in fermentation media for production of recombinant proteins to achieve enhanced bioconversion rates and peptides like insulin and insulin analogues, exendin and enzymes such as lipase using methanol inducible fungal expression systems such as Pichia.Type: ApplicationFiled: January 23, 2014Publication date: May 22, 2014Applicant: Biocon LimitedInventors: Sanjay Tiwari, Mukesh Babuappa Patale, Saurabh Garg, Mayank Kumar Garg, Sulekha Joshi, Chittnalli Ramegowda Naveen Kumar, Bimal Kumar, Anuj Goel, Harish Iyer
-
Publication number: 20140140999Abstract: The invention provides means and methods for producing one or more Ig-like molecules in a single host cell. Novel CH3 mutations enabling the production of monospecific and/or bispecific Ig-like molecules of interest are also provided.Type: ApplicationFiled: November 15, 2013Publication date: May 22, 2014Applicant: MERUS B.V.Inventors: Cornelis A. DE KRUIF, Linda Johanna Aleida HENDRIKS, Ton LOGTENBERG
-
Publication number: 20140141022Abstract: Multivalent binding peptides, including bi-specific binding peptides, having immunoglobulin effector function are provided, along with encoding nucleic acids, vectors and host cells as well as methods for making such peptides and methods for using such peptides to treat or prevent a variety of diseases, disorders or conditions, as well as to ameliorate at least one symptom associated with such a disease, disorder or condition.Type: ApplicationFiled: March 15, 2013Publication date: May 22, 2014Applicant: EMERGENT PRODUCT DEVELOPMENT SEATTLE, LLCInventors: PETER ARMSTRONG THOMPSON, JEFFREY A. LEDBETTER, MARTHA SUSAN HAYDEN-LEDBETTER, LAURA SUE GROSMAIRE, ROBERT BADER, WILLIAM BRADY
-
Publication number: 20140141489Abstract: The present invention relates to variants of a parent alpha-amylase. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.Type: ApplicationFiled: June 29, 2012Publication date: May 22, 2014Inventors: Svend Kaasgaard, Jens Oebro, Signe Eskildsen Larsen, Allan Svendsen, Annette Helle Johansen, Michael Skjoet, Carsten Andersen, Lars Beier, Esben Peter Friis, Miguel Duarte Guilherme Pereira Toscano, Mads Bjoernvad, Frank Winther Rasmussen, Liv Spaanger Christiansen
-
Publication number: 20140134736Abstract: The present disclosure relates to methods of controlling the sulfide (S2˜) content in systems, such as oil and gas reservoirs and pipelines, by the use of chlorine oxyanions and microorganisms with (per)chlorate-reducing activity.Type: ApplicationFiled: May 31, 2012Publication date: May 15, 2014Applicant: The Regents of the University of CaliforniaInventor: John D. Coates
-
Publication number: 20140134722Abstract: The present invention provides novel mammalian alpha-kinase proteins: melanoma alpha-kinase (MK), heart alpha-kinase (HK), kidney alpha-kinase (KK), skeletal muscle alpha-kinase (SK), and lymphocyte alpha-kinase (LK). In particular, a novel kinase type is herein provided, characterized by the presence of an alpha-kinase catalytic domain and an ion channel domain. Isolated nucleic acids of the alpha-kinases MK, HK, KK, SK and LK are provided. Methods for making the novel alpha-kinases, cells that express the alpha-kinases and methods for treating an animal in need of either increased or decreased activity of the alpha-kinases are provided.Type: ApplicationFiled: June 17, 2010Publication date: May 15, 2014Inventor: Alexey Ryazanov
-
Publication number: 20140127770Abstract: The invention features methods producing isoprene and a co-product, such as ethanol, 1,3-propanediol, or hydrogen from cultured cells. The invention also provides compositions that include these cultured cells. The invention provides compositions comprising isoprene and ethanol, isoprene and 1,3-propanediol, and isoprene and hydrogen. Additionally, the invention provides methods of co-producing isoprene and ethanol, isoprene and 1,3-propanediol, and isoprene and hydrogen by culturing cells under conditions suitable for co-production of isoprene and ethanol, isoprene and 1,3-propanediol, and isoprene and hydrogen.Type: ApplicationFiled: January 6, 2014Publication date: May 8, 2014Applicants: The Goodyear Tire & Rubber Company, Danisco US Inc.Inventors: Gopal K. CHOTANI, Joseph C. MCAULIFFE, Caroline M. PERES, Karl J. SANFORD, Derek H. WELLS
-
Publication number: 20140121153Abstract: The present invention relates to an isolated polypeptide comprising (a) the amino acid sequence set forth in SEQ ID NO: 21; or (b) an amino acid sequence that is at least 95% identical to SEQ ID NO: 21, and to therapeutic treatments based thereon.Type: ApplicationFiled: January 2, 2014Publication date: May 1, 2014Inventors: Tamara SANDLER, Orly DEVARY
-
Publication number: 20140113352Abstract: The present invention provides compositions and methods for the expression of recombinant ?-glucosidase variants, as well as their use in the production of fermentable sugars from cellulosic biomass.Type: ApplicationFiled: December 19, 2013Publication date: April 24, 2014Applicant: Codexis, Inc.Inventors: Dipnath Baidyaroy, Louis Clark, Lisa M. Newman
-
Publication number: 20140115740Abstract: The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: December 13, 2013Publication date: April 24, 2014Applicants: Novozymes, Inc., Novozymes A/SInventors: Lan Tang, Ye Liu, Junxin Duan, Yu Zhang, Christian Isak Jorgensen, Randall Kramer
-
Publication number: 20140115739Abstract: The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: October 11, 2013Publication date: April 24, 2014Inventors: Suchindra Maiyuran, Randall Kramer, Paul Harris
-
Publication number: 20140113966Abstract: An acetyl xylan esterase variant having perhydrolytic activity is provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. More specifically, a Thermotoga maritima acetyl xylan esterase gene was modified using error-prone PCR and site-directed mutagenesis to create an enzyme catalyst characterized by an increase in specific activity. The variant acetyl xylan esterase may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, and paper pulp processing applications.Type: ApplicationFiled: October 22, 2012Publication date: April 24, 2014Applicant: E. I. DU PONT DE NEMOURS AND COMPANYInventors: ROBERT DICOSIMO, MARK SCOTT PAYNE, JOHN EDWARD GAVAGAN
-
Publication number: 20140113336Abstract: The present invention relates to isolated polypeptides having xylanase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: August 1, 2012Publication date: April 24, 2014Inventor: Nikolaj Spodsberg
-
Publication number: 20140107315Abstract: An object of the present invention is to provide a technique to create novel engineered protein ligands that, when immobilized through a lysine residue (its side chain ?-amino group) which allows for efficient immobilization to a carrier, show the optimum binding capacity and binding efficiency to a target molecule. The present invention provides an engineered protein having a sequence obtained by replacing all the lysine residues in Protein A, which is the most typical protein ligand, with other amino acids, and adding lysine at a terminal; and an affinity separation matrix in which such an engineered protein is immobilized on a water-insoluble carrier by reductive amination or the like. This affinity separation matrix is characterized by its high binding capacity to a target molecule even when the immobilized amount of the ligand is small.Type: ApplicationFiled: March 26, 2012Publication date: April 17, 2014Inventors: Shinichi Yoshida, Dai Murata, Shunichi Taira
-
Publication number: 20140106410Abstract: The present invention relates to isolated polypeptides having xylanase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: December 20, 2013Publication date: April 17, 2014Applicant: NOVOZYMES, INC.Inventor: Nikolaj Spodsberg
-
Publication number: 20140106406Abstract: The present disclosure relates to the molecular cloning of a gougerotin biosynthetic gene cluster from Streptomyces microflavus, and identification of individual genes in the gene cluster as well as the proteins encoded thereby. A gougerotin gene cluster comprising 13 open reading frames (ORFs) is located within a genetic locus of Streptomyces microflavus. The gougerotin gene cluster further comprises another 11 ORFs, which are also disclosed. Gougerotin analogs and methods for producing them by manipulation of the gougerotin gene cluster and the genes therein are also disclosed.Type: ApplicationFiled: October 15, 2013Publication date: April 17, 2014Applicant: Bayer Cropscience LPInventor: Daniel M. JOO
-
Publication number: 20140105862Abstract: The present invention relates to novel strains of Streptomyces microflavus and methods of their use for controlling diseases or pests of a plant. The invention also relates to a fermentation broth obtained by cultivating a gougerotin producing Streptomyces strain, wherein the fermentation broth contains at least about 1 g/L gougerotin. The invention also relates to a method of producing a fermentation broth of a gougerotin producing Streptomyces strain, wherein the fermentation broth contains at least about 1 g/L gougerotin, the method comprising cultivating the Streptomyces strain in a culture medium containing a digestible carbon source and a digestible nitrogen source under aerobic conditions, wherein the culture medium contains an amino acid at a concentration effective to achieve a gougerotin concentration of at least 1 g/L.Type: ApplicationFiled: October 11, 2013Publication date: April 17, 2014Applicant: Bayer CropScience LPInventors: Brian CAMPBELL, Damian CURTIS, Shaohua GUAN, Magalie GUILHABERT-GOYA, Daniel M. JOO, Tara LU, Jonathan S. MARGOLIS, Reed Nathan ROYALTY, Gerardo Bueno SALAZAR, David SESIN, Frisby Davis SMITH, Colleen TAYLOR, Hong ZHU
-
Publication number: 20140106457Abstract: The present invention relates to a method for constructing a host cell expressing a polypeptide of interest from at least two ORF's stably integrated onto the chromosome of the host cell comprising the steps of: a) providing the at least two ORF's encoding the same polypeptide, wherein the at least two DNA sequences of the ORF's differ in at least one position; b) integrating the at least two ORF's in the same orientation on the host cell chromosome.Type: ApplicationFiled: September 23, 2013Publication date: April 17, 2014Inventors: Steen Troels Jorgensen, Poul Erik Pedersen
-
Publication number: 20140105888Abstract: Novel modulators, including antibodies and derivatives thereof, and methods of using such modulators to treat hyperproliferative disorders are provided.Type: ApplicationFiled: February 17, 2012Publication date: April 17, 2014Applicant: Stem CentRx, Inc.Inventors: Orit Foord, Scott J. Dylla, Robert A. Stull, Alex Bankovich, Alexandra Leida Liana Lazetic, Jeffrey Bernstein
-
Publication number: 20140106399Abstract: The present invention relates to a method for production and purification of polypeptides. In particular, the present invention relates to a fusion protein comprising a solubility-enhancing peptide tag moiety, a self-aggregating peptide moiety and a moiety of target peptide and to a method for production and purification of target peptides through expressing said fusion protein.Type: ApplicationFiled: March 15, 2013Publication date: April 17, 2014Inventors: Zhanglin LIN, Wanghui XU, Lei XING, Lingli ZENG, Bihong ZHOU, Qing ZHAO, Wei WU
-
Patent number: 8697403Abstract: Disclosed are processes for producing lipids for biofuel or lubricant and Streptomyces bacteria used in the processes. The processes include steps whereby bacterial cells of the germs Streptomyces are cultivated in a medium comprising organic waste(s) or residue(s) or mixtures thereof as carbon and/or nutrient source(s), wherein the organic waste(s) or residue(s) comprise lignocellulosic waste(s) or lignocellulosic residue(s), recovering lipids from the cells of the bacteria or from the cultivation medium, and using the recovered lipids or a fraction thereof as biofuel and/or lubricant, or as a starting material for biofuel and/or lubricant production.Type: GrantFiled: May 24, 2011Date of Patent: April 15, 2014Assignee: Neste Oil OyjInventors: Maria Holmback, Miina Lehesto, Perttu Koskinen, Johan-Fredrik Selin
-
Publication number: 20140101785Abstract: Genetically encoded calcium indicator (GECI) polypeptides and the nucleic acid molecules encoding such polypeptides are provided. In addition, methods of using such nucleic acids and polypeptides in methods of screening for agonists or antagonists of G-protein coupled receptor (GPCR) or ion channels and methods of monitoring neural activity also are provided.Type: ApplicationFiled: March 13, 2013Publication date: April 10, 2014Applicant: HOWARD HUGHES MEDICAL INSTITUTEInventors: Loren Looger, Karel Svoboda, Douglas Kim, Rex Kerr
-
Publication number: 20140099678Abstract: Novel fungal endoglucanases Cel5 and Cel12 are disclosed. The endoglucanases are conveniently produced by recombinant technology, and means for their production are described. The endoglucanases are used for treating cellulosic material, especially in textile industry, e.g. in biofinishing or biostoning. They may also be used in detergents, in animal feed and/or in pulp and paper industry, or in hydrolysis of lignocellulosic material for, e.g. bioethanol production.Type: ApplicationFiled: October 18, 2013Publication date: April 10, 2014Applicant: AB Enzymes OyInventors: Terhi PURANEN, Leena VALTAKARI, Marika ALAPURANEN, George SZAKACS, Pentti OJAPALO, Jari VEHMAANPERA