Bacillus (e.g., B. Subtilis, B. Thuringiensis, Etc.) Patents (Class 435/252.5)
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Publication number: 20080009054Abstract: This invention is referred to a new bacterial strain of Bacillus sp., to be applied in a metal biosorption process, which was deposited in the Agricultural Research Culture Collection (NRRL) International Depositary Authority, 1815 N. University Street, Peoria Ill. 61604, USA, having an accession number NRRL-B-30881. It is described, also, a sporulated and non-sporulated industrial inoculant of said bacterium, a method to produce said inoculant, and a process to remove metals by said bacterium.Type: ApplicationFiled: June 26, 2007Publication date: January 10, 2008Applicants: UNIVERSIDAD DE CHILE, BIOTECNOLOGIAS DEL AQUA LTDA.Inventors: Cotoras Davor, Viedma Pabla
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Patent number: 7285406Abstract: The invention relates to the isolation, sequencing, and recombinant expression of genes encoding either a nitrile hydratase (NHase) or amidase (Am) from Comamonas testosteroni 5-MGAM-4D, where the NHase is useful for catalyzing the hydration of nitrites to the corresponding amides, and the amidase is useful for hydrolysis of amides to the corresponding carboxylic acids. Also provided are transformed host cells containing polynucleotides for expressing the nitrile hydratase or amidase enzymes from Comamonas testosteroni 5-MGAM-4D.Type: GrantFiled: November 16, 2006Date of Patent: October 23, 2007Assignee: E.I. du Pont de Nemours and CompanyInventors: Mark S. Payne, Robert DiCosimo, John E. Gavagan, Robert D. Fallon
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Patent number: 7262047Abstract: The present invention relates to discovery and isolation of a biologically pure culture of a Bacillus pumilus SAFR-032 isolate with UV sterilization resistant properties. This novel strain has been characterized on the basis of phenotypic traits, 16S rDNA sequence analysis and DNA-DNA hybridization. According to the results of these analyses, this strain belongs to the genus Bacillus. The GenBank accession number for the 16S rDNA sequence of the Bacillus pumilus SAFR-032 isolate is AY167879.Type: GrantFiled: May 6, 2005Date of Patent: August 28, 2007Assignee: California Institute of TechnologyInventor: Kasthuri J. Venkateswaran
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Patent number: 7250280Abstract: The production of biomass or a desired product from a cell can be improved by inducing conversion of ATP to ADP without primary effects on other cellular metabolites or functions which is achieved by expressing an uncoupled ATPase activity in the cell and incubating the cell with a suitable substrate to produce the biomass or product. This is conveniently done by expressing in the cell the soluble part (F1) of the membrane bound (F0F1 type) H+ ATPase or a portion of F1 exhibiting ATPase activity. The organism from which the F1 ATPase or portions thereof is derived, or in which the F1 ATPase or portions thereof is expressed, may be selected from prokaryotes and eukaryotes.Type: GrantFiled: March 7, 2005Date of Patent: July 31, 2007Inventors: Peter Ruhdal Jensen, Jacky Leendert Snoep, Hans Victor Westerhoff
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Patent number: 7247299Abstract: Antimicrobial compounds from Bacillus subtilis for use against animal and human pathogens. A novel strain of Bacillus subtilis was isolated from the gastrointestinal tract of poultry and was found to produce a factor or factors that have excellent inhibitory effects on Clostridium perfringens, Clostridium difficile, Campylobacter jejuni, Campylobacter coli, and Streptococcus pneumoniae. The factor(s) retain full viability and antimicrobial activity after heat treatment. The invention provides a method of treatment of pathogenic microorganisms including C. perfringens.Type: GrantFiled: November 27, 2002Date of Patent: July 24, 2007Assignee: Kemin Industries, Inc.Inventors: Angeline Seah Huay Lin, Alex Yeow-Lim Teo, Tan Hai Meng
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Patent number: 7241606Abstract: The object of the present invention is to provide an ?-isomaltosylglucosaccharide-forming enzyme, process of the same, cyclotetrasaccharide, and saccharide composition comprising the saccharide which are obtainable by using the enzyme; and is solved by establishing an ?-isomaltosylglucosaccharide-forming enzyme which forms a saccharide, having a glucose polymerization degree of at least three and having both the ?-1,6 glucosidic linkage as a linkage at the non-reducing end and the ?-1,4 glucosidic linkage other than the linkage at the non-reducing end, by catalyzing the ?-glucosyl-transfer from a saccharide having a glucose polymerization degree of at least two and having the ?-1,4 glucosidic linkage as a linkage at the non-reducing end without substantially increasing the reducing power; ?-isomaltosyl-transferring method using the enzyme; method for forming ?-isomaltosylglucosaccharide; process for producing a cyclotetrasaccharide having the structure of cyclo{?6)-?-D-glucopyranosyl-(1?3)-?-D-glucopyranosyl-Type: GrantFiled: July 25, 2001Date of Patent: July 10, 2007Assignee: Kabushiki Kaisha Hayashibara Seibutsu Kagaku KenkyujoInventors: Michio Kubota, Keiji Tsusaki, Takanobu Higashiyama, Shigeharu Fukuda, Toshio Miyake
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Patent number: 7238523Abstract: The invention comprises plasmids and viral vectors containing an animal p53as cDNA sequence. A portion of the p53as sequence may be identified to a position of wild type p53 gene from the same animal. In preferred embodiments, the p53as is mouse or human p53as. A preferred viral vector is baculovirus vector. The invention further includes antibodies both polyclonal and monoclonal, to p53as and to at least a portion of human p53 intron 10 sequence encoding SLRPFKALVREKGHRPSSHSC (SEQ. I.D. NO. 1) which is related to p53as sequences and plasmids and viral vectors containing such sequences. All of the above find utility in studying p53 and p53as and their relative expressions which is believed important for detection and control of malignant cells and their susceptibility to treatment agents.Type: GrantFiled: May 10, 1996Date of Patent: July 3, 2007Assignee: Health Research, Inc.Inventor: Molly F. Kulesz-Martin
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Patent number: 7232571Abstract: The invention describes therapeutic compositions including a lactic acid-producing bacteria, such as isolated Bacillus coagulans, in combination with a bifidogenic oligosaccharides or other cholesterol-reducing agents for use in reducing LDL cholesterol and serum triglycerides. Also described are therapeutic methods using the compositions and systems containing the therapeutic compositions.Type: GrantFiled: November 1, 2004Date of Patent: June 19, 2007Assignee: Ganeden Biotech, Inc.Inventors: Scan Farmer, Andrew R. Lefkowitz
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Patent number: 7217414Abstract: The present invention relates to the use of lactic acid bacteria capable of adhering to the mucosa of the intestine and especially colonizing it for the prevention of peritonitis. In particular, the present invention relates to the use of such lactic acid bacteria for the prevention of peritonitis caused by cirrhosis of the liver. Specifically, the present invention relates to a method for preventing peritonitis in a patient in need of such prevention. This method includes administering to the patient a lactic acid bacterium that is capable of adhering to the intestine's mucosa and essentially colonizing it for the preparation of an ingestable carrier. The invention also relates to a peritonitis preventing composition of a lactic acid bacterium that is capable of adhering to the intestine's mucosa and essentially colonizing it for the preparation of an ingestable carrier. The carrier is preferably a food or pharmaceutical composition.Type: GrantFiled: September 20, 2002Date of Patent: May 15, 2007Assignee: Nestec S.A.Inventors: Eduardo Schiffrin, Carlos Guarner, German Soriano
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Patent number: 7214792Abstract: A novel microorganism producing a nontoxic, non-antigenic exopolysaccharide is taught. The exopolysaccharide has neutral sugars migrating at the same rate as mannose, fucose, fructose and galactose, acidic sugars migrating at the same rate as fucose and amine sugars migrating at the same rate as glucose and fucose, and wherein the ratio of galactose:fucose:glucose:mannose is about 1:2:3:6. The microbe and the exopolysaccharide have uses as a biofilm in geologic applications and have several consumer uses as food and drug polymers and use as a plasma extender.Type: GrantFiled: October 8, 2003Date of Patent: May 8, 2007Assignee: The Board of Regents, The University of Texas SystemInventors: Lee A. Bulla, John P. Turner
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Patent number: 7214521Abstract: The present invention relates to a novel inulin synthase having a function and a substrate specificity of acting on sucrose to produce inulin, but not acting on kestose, maltose, lactose, trehalose and cellobiose; and a process for producing inulin comprising the step of allowing the synthase, a culture fluid or cultured cells of a microorganism producing the synthase, or a treated product thereof to contact with sucrose to produce inulin.Type: GrantFiled: February 16, 2001Date of Patent: May 8, 2007Assignee: Fuji Nihon Seito CorporationInventors: Tadashi Wada, Masao Ohguchi
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Patent number: 7214525Abstract: A method for killing pests (e.g. insects) comprising administering material from Xenorhabdus species (e.g. X. nematophilus) such as cells or supernatants orally to the pests, either alone or in conjunction with Bacillus thuringiensis or pesticidal materials derived therefrom. Also disclosed is an isolated pesticidal agent (and compositions comprising the same) characterized in that it is obtainable from cultures of X. nematophilus or mutants thereof, has oral pesticidal activity agent Pieris brassicae, Pieris rapae and Plutella xylostella, is substantially heat stable to 55° C., is proteinaceous, acts synergistically with B. thuringiensis cells as an oral pesticide and is substantially resistant to proteolysis by trypsin and proteinase K. DNA encoding pesticidal activity is also disclosed.Type: GrantFiled: August 27, 1997Date of Patent: May 8, 2007Assignee: University of WarwickInventors: Paul Jarrett, Deborah June Ellis, James Alun Wynne Morgan
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Patent number: 7211428Abstract: The present invention relates to the selection and development of superior strain of Bacillus spp for improving plant growth and health by inhibiting pathogenic fungi.Type: GrantFiled: May 18, 2004Date of Patent: May 1, 2007Assignee: Council of Scientific and Industrial ResearchInventors: Abdul Sattar, Mansoor Alam, Abdul Khaliq, Suman Preet Singh Khanuja, Alok Kalra, Abdul Samad, Ajit Kumar Shasany, Mahendra Pandurang Darokar, Ashutosh Kumar Shukla, Togarati Padmapriya, Mohammad Yaseen, Om Parkash Dhawan, Mohammad Zaim, Poovappallivadakethil Viswanathan Nair Ajaya Kumar
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Patent number: 7202077Abstract: The increased use of nucleotide sequence data mining techniques has amplified the demand for efficient methods of producing recombinant proteins in prokaryotic cells. A strategy is provided for enhancing the synthesis of recombinant amino acid sequences by improving translation from expression cassettes in vitro before producing recombinant hosts.Type: GrantFiled: April 12, 2002Date of Patent: April 10, 2007Assignee: ZymoGenetics, Inc.Inventors: Tracey A. Pownder, Chung Chan
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Patent number: 7192746Abstract: The object of the present invention is to provide an ?-isomaltosyl-transferring enzyme which forms a cyclotetrasaccharide having the structure of cyclo{?6)-?-D-glucopyranosyl-(1?3)-?-D-glucopyranosyl-(1?6)-?-D-glucopyranosyl-(1?3)-?-D-glucopyranosyl-(1?} from a saccharide having a glucose polymerization degree of at least three and having both the ?-1,6 glucosidic linkage as a linkage at the non-reducing end and the ?-1,4 glucosidic linkage other than the linkage at the non-reducing end; microorganisms which produce the enzyme; process for producing the enzyme; cyclotetrasaccharide or saccharide compositions comprising the same; and uses thereof.Type: GrantFiled: May 22, 2001Date of Patent: March 20, 2007Assignee: Kabushiki Kaisha Hayashibara Seibutsu Kagaku KenkyujoInventors: Michio Kubota, Tomoyuki Nishimoto, Hajime Aga, Shigeharu Fukuda, Toshio Miyake
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Patent number: 7189556Abstract: The present invention relates to discovery and isolation of a biologically pure culture of a Bacillus odysseyi isolate with high adherence and sterilization resistant properties. B. odysseyi is a round spore forming Bacillus species that produces an exosporium. This novel species has been characterized on the basis of phenotypic traits, 16S rDNA sequence analysis and DNA-DNA hybridization. According to the results of these analyses, this strain belongs to the genus Bacillus and the type strain is 34hs-1T (=ATCC PTA-4993T=NRRL B-30641T=NBRC 100172T). The GenBank accession number for the 16S rDNA sequence of strain 34hs-1T is AF526913.Type: GrantFiled: January 17, 2004Date of Patent: March 13, 2007Assignee: California Institute of TechnologyInventors: Kasthuri Venkateswaran, Myron Thomas La Duc
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Patent number: 7183088Abstract: Thermophilic microorganism Bacillus coagulans strain SIM-7 DSM 14043 for the production of L(+)-lactate from fermentable sugars, including dextrines and starch. The temperature of cultivation is 53–65° C. being the optimal. The final concentration of L(+)-lactate in fermentation is 12% with the yield of 95%. The cultivation of this strain of microorganism is possible without the high-temperature sterilization of equipment and media. Using cereal flour as the source of fermentable sugars, the need of the strain of the microorganism for mineral and nitrogen salts will be covered by the compounds present in the cereals.Type: GrantFiled: March 15, 2002Date of Patent: February 27, 2007Assignee: The University of TartuInventors: Aida Simisker, legal representative, Allan Nurk, Ain Heinaru, Jaan Simisker, deceased
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Patent number: 7166456Abstract: Riboflavin-producing Bacillus subtilis which is resistant to proline analogue, and a method for producing riboflavin using the Bacillus subtilis are provided.Type: GrantFiled: June 4, 2003Date of Patent: January 23, 2007Assignee: CJ Corp.Inventors: Kwang Ho Lee, Young Hoon Park, Jong Kwon Han, Jang Hee Park, Kyung Han Lee, Hyang Choi
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Patent number: 7157244Abstract: The present invention provides an isolated mammalian adenosine receptor, isolated or recombinant nucleic acids and recombinant vectors encoding the same, host cells comprising the nucleic acids and vectors, and methods of making the receptor using the host cells. This invention further provides antibodies and antigen binding fragments thereof which specifically bind to the receptor and are useful for treating medical conditions caused or mediated by adenosine. Also provided are screening methods for identifying specific agonists and antagonists of the mammalian adenosine receptor.Type: GrantFiled: January 17, 2001Date of Patent: January 2, 2007Assignee: Schering CorporationInventors: Joseph A. Hedrick, Jean E. Lachowicz, Wei Wang, Eric L. Gustafson
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Patent number: 7148042Abstract: A glyoxal-guanosine-group compound is prepared either by reacting glyoxal-guanine with any one of ribose-1-phosphate and 2-deoxyribose-1-phosphate in the presence of purine nucleoside phosphorylase, or by reacting glyoxal-guanine with any one selected from the group consisting of uridine, 2?-deoxyuridine and thymidine, together with phosphate ion, in the presence of purine nucleoside phosphorylase and pyrimidine nucleoside phosphorylase. The glyoxal-guanosine-group compound is then decomposed by alkali, whereby a guanosine-group compound consisting of guanosine and 2?-deoxyguanosine is prepared.Type: GrantFiled: July 8, 2003Date of Patent: December 12, 2006Assignee: Yuki Gosei Kogyo Co., Ltd.Inventors: Yoichi Mikami, Seiichiro Matsumoto, Yoshinori Hayashi, Toyoki Sato
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Patent number: 7141396Abstract: A glyoxal-guanosine-group compound is prepared either by reacting glyoxal-guanine with any one of ribose-1-phosphate and 2-deoxyribose-1-phosphate in the presence of purine nucleoside phosphorylase, or by reacting glyoxal-guanine with any one selected from the group consisting of uridine, 2?-deoxyuridine and thymidine, together with phosphate ion, in the presence of purine nucleoside phosphorylase and pyrimidine nucleoside phosphorylase. The glyoxal-guanosine-group compound is then decomposed by alkali, whereby a guanosine-group compound consisting of guanosine and 2?-deoxyguanosine is prepared.Type: GrantFiled: July 8, 2003Date of Patent: November 28, 2006Assignee: Yuki Gosei Kogyo Co., Ltd.Inventors: Yoichi Mikami, Seiichiro Matsumoto, Yoshinori Hayashi, Toyoki Sato
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Patent number: 7141397Abstract: A glyoxal-guanosine-group compound is prepared either by reacting glyoxal-guanine with any one of ribose-1-phosphate and 2-deoxyribose-1-phosphate in the presence of purine nucleoside phosphorylase, or by reacting glyoxal-guanine with any one selected from the group consisting of uridine, 2?-deoxyuridine and thymidine, together with phosphate ion, in the presence of purine nucleoside phosphorylase and pyrimidine nucleoside phosphorylase. The glyoxal-guanosine-group compound is then decomposed by alkali, whereby a guanosine-group compound consisting of guanosine and 2?-deoxyguanosine is prepared.Type: GrantFiled: July 8, 2003Date of Patent: November 28, 2006Assignee: Yuki Gosei Kogyo Co., Ltd.Inventors: Yoichi Mikami, Seiichiro Matsumoto, Yoshinori Hayashi, Toyoki Sato
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Patent number: 7115725Abstract: Disclosed is DNA encoding diphtheria toxin polypeptides having multiple mutations, which render the polypeptides useful as vaccines.Type: GrantFiled: August 20, 2002Date of Patent: October 3, 2006Assignee: President and Fellows of Harvard CollegeInventor: R. John Collier
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Patent number: 7097830Abstract: The present invention relates to a synergistic composition useful as bioinoculant, said composition comprising bacterial strains of accession Nos. NRRL B-30486, NRRL B-30487, and NRRL-B 30488, individually or in all possible combinations, and optionally carrier, with each of the strains showing plant promotery activity, phytopathogenic fungi controlling activity, abiotic stress conditions tolerating capability, phosphate solubilization capability under abiotic stress conditions; further, a method of producing said composition thereof, and in addition, a method of isolating said bacterial strains from cow ‘Sahiwal’.Type: GrantFiled: June 19, 2002Date of Patent: August 29, 2006Assignee: Council of Scientific and Industrial ResearchInventors: Chandra Shekhar Nautiyal, Sangeeta Mehta, Harikesh Bahaadur Singh, Palpu Pushpangadan
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Patent number: 7098009Abstract: The subject invention relates to newly isolated organisms from nature that produce L(+)-lactic acid high yield from hexose and pentose sugars found in biomass. Organisms and processes or methods for the production of lactic acid and other industrially important chemicals from cellulose and hemicellulose are also provided.Type: GrantFiled: March 4, 2004Date of Patent: August 29, 2006Assignee: University of Florida Research Foundation, Inc.Inventors: Keelnatham T. Shanmugam, Lonnie O'Neal Ingram, Milind A. Patel, Mark S. Ou, Roberta Harbrucker
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Patent number: 7094592Abstract: The present invention relates to a novel strain of Bacillus sp. D747 (deposited as FERM BP-8234) and methods for controlling plant diseases and insect pests, comprising administering cultures of Bacillus sp. D747 (including the viable bacteria) or viable bacteria isolated by culturing, on the plant parts such as roots, stems, leaves, seeds, and the like, or in the culture soil.Type: GrantFiled: November 26, 2002Date of Patent: August 22, 2006Assignee: Kumiai Chemical Industry Co., Ltd.Inventors: Satoshi Watanabe, Jun Toyoshima, Tsutomu Shimizu, Koji Yamaji, Kozo Nagayama, Hiroyuki Yano
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Patent number: 7091013Abstract: The present invention discloses a process for producing sodium 2-keto-L-gulonate from sorbitol and recovering 2-keto-L-gulonic acid in high recovery yields, by controlled cation exchange treatment of the micro-organism free fermentation broth and/or adjusting PII of said purified fermentation broth, followed by direct crystallisation of 2-keto-L-gulonic acid monohydrate.Type: GrantFiled: August 2, 2002Date of Patent: August 15, 2006Assignee: Cerestar Holding B.V.Inventors: Jean-Claude Marie-Pierre Ghislain De Troostembergh, Ignace André Debonne, Willy Richard Obyn, Catherine Gwenaëlle Michelle Peuzet
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Patent number: 7083955Abstract: Cost-effective processes for producing lactic acid and/or lactate from pentose-containing substrates, such as xylose-containing substrates, are provided. In particular, processes for producing lactic acid and/or lactate include fermentation of pentoses, such as xyloses, to enantiomerically pure lactic acid and/or lactate by moderately thermophilic Bacillus species. This fermentation runs by a homofermentive pathway and produces predominantly C3 compounds, so that C3 compounds can be recovered as lactic acid and/or lactate.Type: GrantFiled: January 13, 2004Date of Patent: August 1, 2006Assignee: Purac Biochem BVInventor: Roel Otto
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Patent number: 7078222Abstract: Riboflavin-producing Bacillus subtilis which is resistant to threonine analogue, and a method for producing riboflavin using the Bacillus subtilis are provided. The subject Bacillus subtilis referred to as Bacillus subtilis CJKB0002 has the accession number KCCM-10446, having been deposited in Korean Culture Center of Microorganisms (KCCM), 361-221, Yurim B/D, Hongie-1-dong, Seodaemun-gut, Seoul 120-091 Republic of Korea, on Nov. 18, 2002.Type: GrantFiled: June 4, 2003Date of Patent: July 18, 2006Assignee: CJ Corp.Inventors: Kwang Ho Lee, Young Hoon Park, Jong Kwon Han, Jang Hee Park, Kyung Han Lee, Hyang Choi
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Patent number: 7056888Abstract: The subject invention provides KB59A4-6 pesticidal proteins and preferred methods of using these proteins to control lepidoteran pests. This invention provides Bacillus thuringiensis isolate KB59A4-6.Type: GrantFiled: May 30, 2003Date of Patent: June 6, 2006Assignee: Dow AgroSciences LLCInventors: Jerald S. Feitelson, H. Ernest Schnepf, Kenneth E. Narva, Brian A. Stockhoff, James Schmeits, David Loewer, Charles Joseph Dullum, Judy Muller-Cohn, Lisa Stamp, George Morrill, Stacey Finstad-Lee
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Patent number: 7030085Abstract: A microorganism belonging to the genus Bacillus is cultured and thiopeptide compounds QN3323 and thiocillin I/II compounds are collected from the culture medium. The compounds are useful as drugs to treat infections caused by multidrug-resistant bacteria.Type: GrantFiled: March 12, 2002Date of Patent: April 18, 2006Assignees: Astellas Pharma INC, National Institute of Advanced Industrial Science and TechnologyInventors: Kazuma Kamigiri, Masato Watanabe, Koji Nagai, Nakako Arao, Kenichi Suzumura, Ken-ichi Suzuki, Ryuichiro Kurane, Masakazu Yamaoka, Yasuhiro Kawano
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Patent number: 7025974Abstract: The invention describes compositions and methods for inhibiting microbial infections associated with the use of sanitary products, such as diapers, bandages, sanitary napkins, tampons and the like. The invention comprises providing for use a sanitary product containing an effective amount of a viable non-pathogenic lactic-acid bacteria, such as Bacillus coagulans, or an extracellular product thereof, useful for inhibiting growth of parasites and pathogens on the epithelial tissue in contact with the sanitary product during use of the product. The invention also provides for enhancing biodegradation of sanitary products after use and disposal. Also described are methods using the product and systems containing the compositions.Type: GrantFiled: April 5, 2004Date of Patent: April 11, 2006Assignee: Ganeden Biotech IncorporatedInventors: Sean Farmer, Andrew R. Lefkowitz
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Patent number: 7026160Abstract: A composition for treatment of renal failure with selected bacteria which converts nitrogenous waste into non-toxic compounds in vivo is provided. Also provided is a method of inhibiting build up of toxins and metabolic wastes and overgrowth of undesirable bacteria in a subject by administering the composition to the subject.Type: GrantFiled: September 30, 2003Date of Patent: April 11, 2006Assignee: Kibow Biotech, Inc.Inventor: Nataragan Ranganathan
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Patent number: 7001755Abstract: Four yeasts (NRRL Y-30213, NRRL Y-30214, NRRL Y-30215, and NRRL Y-30216) and 1 bacterium (NRRL B-30212) have been identified as being superior antagonists capable of suppressing Fusarium head blight (head scab) in cereals, particularly in wheat and barley. Fusarium head blight is primarily caused by the fungus Gibberella zeae (anamorph=Fusarium graminearum).Type: GrantFiled: March 18, 2003Date of Patent: February 21, 2006Assignees: The United States of America, as represented by the Secretary of Agriculture, The Ohio University Research FoundationInventors: David A. Schisler, Naseem I. Khan, Michael J. Boehm
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Patent number: 6989266Abstract: A cement paste containing microbial cells which has a sustained effect and an excellent function of purifying water. This cement paste contains a mixture of Bacillus subtilis, Bacillus thuringiensis and Bacillus sphaericus cells at a mixing ratio by mass of 0.1 to 50:0.1 to 50:0.1 to 50.Type: GrantFiled: December 10, 2001Date of Patent: January 24, 2006Assignee: Koyoh Corporation, Ltd.Inventors: Masayuki Koga, Yoko Koga
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Patent number: 6984406Abstract: The present invention relates to a Bacillus sp. strain producing a mannanase which is highly active in the neutral and the acidic media. The Bacillus sp. WL-1 strain (KCTC 0800BP), which is isolated from the soil, produces in large scale the mannanase in the culture medium containing lactose and bran, wherein the mannanase is highly active in the neutral and the acidic media so said mannanase is useful as an additive for feeds and is helpful in the decomposition of hemicellulose.Type: GrantFiled: June 20, 2001Date of Patent: January 10, 2006Assignee: CTC BIO Inc.Inventors: Ki-Haeng Cho, Ki-Hong Yoon, Dae-Weon Kim, Hwa-Gyun Oh, Young-Phill Oh
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Patent number: 6982095Abstract: Capsules which contain vital cells or tissues as the contents and in which these cells or tissues can be grown. Since these cells or tissues can be grown in the capsules, an extremely high cell density can be achieved. By using these capsules, foods having an excellent effect of ameliorating intestinal disorders, etc. can be provided. Moreover, it is possible to provide artificial seeds showing an excellent storage stability and a high germination ratio which comprise seamless soft capsules consisting of an innermost layer containing indefinite embryo, indefinite shoots, multiple shoots, shoot apexes, growing points, protocorm-like bodies, indefinite roots, hairy roots, etc., an inner coating layer comprising a hardened oil and an outer coating layer made of a biodegradable film comprising gelatin, polysaccharides, etc.Type: GrantFiled: June 28, 2001Date of Patent: January 3, 2006Assignee: Morishita Jintan Co., Ltd.Inventors: Masanori Asada, Yumi Hatano, Ryosei Kamaguchi, Hideki Sunohara
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Patent number: 6974691Abstract: The present invention provides in one aspect for a method for controlling odor associated with spills of organic material which can cause odors on carpets. The method comprises applying to the carpet a preparation of dormant bacteria, which when activated are effective to control odors. The dormant bacterial preparation is allowed to become associated with the carpet, such that when the carpet is exposed to organic material which can cause odors, the bacteria are capable of becoming active and digesting the organic material. The formulation may contain other agents such as odor neutralizing or masking agents, enzymes, foaming or anti-foaming agents.Type: GrantFiled: July 25, 2003Date of Patent: December 13, 2005Assignee: Life Science TGO, S.R.L.Inventors: Jeffrey Kent Fredenburgh, Rae Anne Cordick
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Patent number: 6967087Abstract: The present invention relates to a novel obligately symbiotic thermophile Symbiobacterium toebii SC-1(Accession NO: KCTC 0685BP), and a thermostable L-tyrosine phenol-lyase and L-tryptophan indole-lyase produced by Symbiobacterium toebii SC-1. The present invention also relates to a pure culture method of Symbiobacterium toebii SC-1, a method of growth measurement for Symbiobacterium toebu SC-1 and its relative symbiotic bacteria showing low-growth yield using nitrate respiration, and a screening method of relative symbiotic strains of Symbiobacterium toebii SC-1 from the environment using a specific antibody to it. Therefore, the thermostable L-tyrosine phenol-lyase and L-tryptophan indole-lyase produced by Symbiobacterium toebii SC-1 in this invention can provide more stable catalysts in enzymatic biotransformation processes which enzymatically produce valuable medico-amino acids 3,4-dehydroxy-L-phenylalanine (L-DOPA) and L-tryptophan.Type: GrantFiled: July 14, 2000Date of Patent: November 22, 2005Assignee: Korea Research Institute of Bioscience and BiotechnologyInventors: Moon-Hee Sung, Seung-Goo Lee, Sung-Keun Rhee, Seung-Pyo Hong, Dae-Joong Kang, Su-Mi Kim, Chul-Joong Kim, Jin-Woo Bae, Che Ok Jeon, Joong-Jae Kim, Kwang Kim, Jae Jun Song
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Patent number: 6960342Abstract: The present invention provides a novel Bacillus amyloliquefaciens strain that exhibits broad antifungal activity. The present invention also provides the use of the Bacillus amyloliquefaciens strain or an antifungal composition comprised of the novel strain for control of a broad range of fungal plant pathogens.Type: GrantFiled: September 12, 2002Date of Patent: November 1, 2005Assignee: National Taiwan UniversityInventors: Wen-Shi Wu, An-Long Chiou
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Patent number: 6929940Abstract: The subject invention pertains to polynucleotides encoding the enzyme oxalate decarboxylase from the filamentous fungus Aspergillus niger and methods of use. The subject invention also pertains to methods of using the enzyme oxalate decarboxylase from Bacillus subtilis.Type: GrantFiled: August 20, 2003Date of Patent: August 16, 2005Assignee: University of Florida Research Foundation, Inc.Inventors: Nigel Gordon John Richards, Christopher Harry Chang, Ammon B. Peck
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Patent number: 6905864Abstract: Polyaromatic hydrocarbons are found in contaminated soils and groundwater and are a large class of compounds for which treatment by bioremediation is sought. Accordingly, indigenous organisms that can degrade polyaromatic hydrocarbon are needed. Presented are strains of the Paenibacillus genus that degrade low molecule weight polyaromatic, and, in the presence of phenanthrene, high molecular weight polyaromatic hydrocarbons. These bacteria strains are unique among PAH-degrading bacterial strains because they form endospores and therefore can survive adverse environmental conditions. Among the strains presented are several from “P. naphthalenovorans”, a newly described species. Also presented are compositions for bioremediation comprising the bacterial strains of the invention, and a method for isolating the strains and a method for using the strains for bioremediation.Type: GrantFiled: May 24, 2002Date of Patent: June 14, 2005Assignee: Rutgers, the State UniversityInventors: Max M. Haggblom, Lori Daane
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Patent number: 6896883Abstract: The present invention is directed to isolated Bacillus subtilis, Pseudomonas putida, and Sporobolomyces roseus which are useful as a biocontrol agent. These organisms are useful in a method of imparting to plants protection against plant pathogens by applying them to plants, plant seeds, or soil surrounding plants under conditions effective to impart disease protection to the plants or plants produced from the plant seeds. The biocontrol agents are also useful in a method of enhancing plant growth which involves applying them to plants, plants seeds, or soil surrounding plants under conditions effective to enhance growth in the plants or plants produced from the plant seeds.Type: GrantFiled: September 10, 2002Date of Patent: May 24, 2005Assignees: Cornell Research Foundation, Inc., Embrapa TrigoInventors: Gary C. Bergstrom, Wilmar Corio da Luz
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Patent number: 6884351Abstract: The present invention is directed to methods of remediating sewage matter, reducing the amount of sludge in sewage and reducing odor-causing matter in sludge. The invention is further directed to compositions having specific genetic characteristics useful in bioremediation of sewage matter and methods of producing same.Type: GrantFiled: May 7, 2003Date of Patent: April 26, 2005Inventor: Mary L. Lytal
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Patent number: 6878373Abstract: A composition and method for reducing the levels of pathogenic bacteria in an aquatic environment. The composition includes isolated Bacillus cereus which are able to reduce a number of well known pathogenic bacterium from aquatic environments. The preferred Bacillus cereus is strain RRRL B-30535.Type: GrantFiled: December 3, 2001Date of Patent: April 12, 2005Assignee: MicroPure Technologies, Inc.Inventors: Jimmie A. Keeton, Diane P. Williams
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Patent number: 6846664Abstract: The present invention relates to a novel strain of alkalothermophilic Bacillus sp. isolated from a hot spring at Vajeshwari, District Thane, The State of Maharashtra, India and deposited at American Type Culture Centre (ATCC), bearing accession No. PTA 972, said strain of Bacillus sp. having the following characteristics (i) aerobic, (ii) gram positive, (iii) motile, (iv) spore forming, (v) capable of growing in a alkaline medium at pH 8-10, and (vi) exhibiting negative reaction towards production of indole, hydrogen, sulfide, ammonia and urease and positive reaction for hydrolysis of starch, production of catalase, hydrolysis of casein and reduction of nitrate.Type: GrantFiled: June 12, 2002Date of Patent: January 25, 2005Assignee: Council for Scientific and Industrial ResearchInventors: Chandravanu Dash, Sangita Uday Phadtare, Absar Ahmad, Vasanti Vishnu Deshpande, Mala Balchandra Rao
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Patent number: 6844182Abstract: This invention is directed to Bacillus mutants which produce a factor which potentiates the pesticidal activity of a Bacillus related pesticide, a chemical pesticide and/or a virus with pesticidal properties.Type: GrantFiled: May 15, 2001Date of Patent: January 18, 2005Assignee: Valent BioSciences CorporationInventors: Helle Outtrup, William D. Lidster, Susan C. MacIntosh, Robert L. Starnes, Denise Carol Manker
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Patent number: 6841366Abstract: The invention generally provides the genes of the biotin biosynthetic operon of Bacillus subtilis, and closely related species thereof, and well as constructions useful for high level production of biotin.Type: GrantFiled: September 28, 1999Date of Patent: January 11, 2005Assignee: DSM IP Assets B.V.Inventors: Stanley Grant Bower, John B. Perkins, R. Rogers Yocum, Janice G. Pero
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Publication number: 20040219651Abstract: The present invention relates to a novel flocculant comprising bacteria of the Bacillus genus cultivated in a culture medium comprising soybean protein, glucose, molasses, and yeast. Alternatively, the flocculant comprises soybean protein. The present invention also relates to methods of making the flocculant, including fermentation method for mass production, and methods of using the flocculant in water treatment.Type: ApplicationFiled: January 21, 2004Publication date: November 4, 2004Inventors: Heng-Chuan Wang, Ming-Hsin Li, Hsu-Wen Tsang, Mei-Mao Wu, Hsui-Ping Pearl Lin
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Patent number: 6812022Abstract: Microorganisms of the genus Bacillus that are capable of reducing nitrates and contain chitin and/or chitosan in their cell walls are provided. The microorganism of the invention can be used for improving soil, for treating organic waste by fermentation, for fermenting soybeans, and for reducing bitterness. The microorganism of the invention can also be used as a feed additive or a good additive. Further, the microorganism of the invention has an anti-microbial effect.Type: GrantFiled: October 16, 2001Date of Patent: November 2, 2004Assignee: Gold Kosan Co., Ltd.Inventor: Takemi Aonuma