Bacillus (e.g., B. Subtilis, B. Thuringiensis, Etc.) Patents (Class 435/252.5)
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Publication number: 20110027246Abstract: A novel bacterial strain of Bacillus thuringiensis for inhibiting insect pests is provided, wherein the Bacillus thuringiensis includes the gene fragments of cry1Ab, cry1Ac, cry1D, and cry1E.Type: ApplicationFiled: September 22, 2009Publication date: February 3, 2011Inventors: Ching-Chou TZENG, Sheueh Kuo, Suey-Sheng Kao
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Publication number: 20110030102Abstract: Engineered polypeptides useful in synthesizing acyl amino acids are provided. Also provided are methods of making acyl amino acids using engineered polypeptides. In certain embodiments, an acyl amino acid produced using compositions and/or methods of the present invention comprises cocoyl glutamate.Type: ApplicationFiled: April 16, 2008Publication date: February 3, 2011Inventors: Kevin A. Jarrell, Prashanth Vishwanath, Gabriel Reznik
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Publication number: 20110009476Abstract: Compositions and methods for identifying and using cis-regulatory and decoy sequences.Type: ApplicationFiled: October 3, 2008Publication date: January 13, 2011Inventor: Michael McArthur
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Publication number: 20110010806Abstract: The present disclosure relates to a method of directing the evolution of an organism by modifying the mutation rate of an organism. The increase in genetic diversity may be used to facilitate the selection of a desired hereditary trait in an organism.Type: ApplicationFiled: June 10, 2009Publication date: January 13, 2011Applicant: NEO-MORGAN LABORATORY INCORPORATEDInventors: Masanori Ogawa, Takayuki Horiuchi, Kazufumi Tabata, Shuntaro Yano, Michi Kubota, Eri Kurita, Akiko Itadani, Sanae Fukushima-Tanaka
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Patent number: 7854927Abstract: The present invention describes the use of lactic acid bacteria, particularly lactic acid producing members of the genus Bacillus, in treating digestive-related immune disorders by downregulating of cytokines and by inhibiting pathogenic or deleterious microorganisms in the gastrointestinal tract. Specific formulations of Bacillus coagulans for various immune disorders are elaborated.Type: GrantFiled: May 10, 2005Date of Patent: December 21, 2010Assignee: Ganeden Biotech, Inc.Inventors: Sean Farmer, Andrew R. Lefkowitz
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Patent number: 7854926Abstract: A biological soil nutrient system that combines beneficial soil fungi and bacteria in a growth promoting nutrient medium, embedded in an inorganic porous ceramic particle for direct delivery during soil aerification to the rhizosphere of adventitious plants, including sports turf, landscape and agricultural applications.Type: GrantFiled: January 4, 2007Date of Patent: December 21, 2010Assignee: Profile Products L.L.C.Inventor: Kevin S. Spittle
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Patent number: 7850958Abstract: A recombinant double stranded RNA (dsRNA) nucleocapsid useful for the expression of dsRNA expression cassettes encoding passenger genes, such as, but not restricted to, vaccine antigens, bioactive proteins, immunoregulatory proteins, antisense RNAs, and catalytic RNAs, replicates in bacterial hosts and includes a P8 protein shell and three dsRNA segments where one of the segments includes a cap independent translation enhancer (CITE) operationally linked to a passenger gene.Type: GrantFiled: July 1, 2009Date of Patent: December 14, 2010Assignee: University of Maryland Biotechnology InstituteInventor: David Hone
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Publication number: 20100311135Abstract: The invention provides: an isopropyl alcohol-producing bacterium which has an acetoacetate decarboxylase activity, an isopropyl alcohol dehydrogenase activity, a CoA transferase activity and a thiolase activity having been imparted thereto and is capable of producing isopropyl alcohol from a plant-derived material; a method of producing isopropyl alcohol whereby isopropyl alcohol is produced from a plant-derived material by using this isopropyl alcohol-producing bacterium; and an apparatus therefor.Type: ApplicationFiled: July 4, 2008Publication date: December 9, 2010Inventors: Nozomi Takebayashi, Mitsufumi Wada, Daisuke Mochizuki, Fuminobu Yoshimi, Seiichi Watanabe, Hitoshi Takahashi, Takashi Morishige
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Publication number: 20100304379Abstract: The presently-disclosed subject matter is directed to biosensors comprising spore-forming bacterial cells and/or spores generated therefrom, a recognition unit within each spore-forming cell for binding an analyte of interest, and a reporter molecule within each spore-forming cell for detecting binding of the analyte of interest, wherein the reporter molecule generates a detectable signal upon binding of the analyte by the recognition element. The presently-disclosed subject matter further provides methods of using the biosensors and systems and kits including the biosensors.Type: ApplicationFiled: September 10, 2008Publication date: December 2, 2010Inventors: Sylvia Daunert, Sapna K. Deo, Patrizia Pasini, Amol Date
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Patent number: 7842494Abstract: The present invention relates to product(s) containing living microorganism(s) suitable for soil treatment, microorganisms multiplying under different climatic and natural circumstances, as well as procedures for the production of the products, and procedures for the treatment of the soil and plants with the products. More particularly, the invention relates to a procedure for preparing the products from any of the microorganisms specified below, or from the mixture thereof. Furthermore, the invention relates to a procedure for the creation of the cultures of the microorganisms to be used. The subject invention also pertains to the microorganisms themselves. More particularly, the invention relates to a procedure for the treatment of the soil and the plants with a product containing at least one of the microorganisms selected from Azospirillum brasilense ssp. SW51 (NCAIM /P/ B 001293), Azotobacter vinelandii ssp. M657 (NCAIM /P/B 001292), Pseudomonas fluorescens var.Type: GrantFiled: August 12, 2002Date of Patent: November 30, 2010Assignee: Agro.Bio Hungary KFT.Inventors: Gyorgy Botond Kiss, Istvan Ott
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Publication number: 20100297703Abstract: This invention relates to inhibition of microbial lysis via regulation of activity of a lytic enzyme for degrading the cell wall. The lytic enzyme inhibitor and the lysis inhibitor are each composed mainly of the Bacillus subtilis-derived YoeB gene or the YoeB protein encoded by a gene homologous to the YoeB gene. An example of the YoeB protein is a protein consisting of the amino acid sequence as shown in SEQ ID NO: 2.Type: ApplicationFiled: October 16, 2007Publication date: November 25, 2010Inventors: Katsutoshi Ara, Junichi Sekiguchi, Hiroki Yamamoto, Hiroyuki Harada
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Publication number: 20100291264Abstract: [Object] To provide a strain in natto-producing bacteria that is suitable for the production of soft natto (a fermented soybean product). [Means for Solving the Problems] A strain in natto-producing bacteria that is suitable for the production of soft natto is provided by isolating natto-producing strains from chungkookjang, which is a fermented food having been taken in Korea, and selecting an appropriate natto-producing strain using the stickiness and hardness of natto as indications. Different from the existing natto-producing bacteria, this strain is characterized by being capable of softening natto with the progress of the fermentation. Also, it is intended to provide a method of producing soft natto by using the above-described strain and the soft natto produced by this production method.Type: ApplicationFiled: April 21, 2008Publication date: November 18, 2010Applicants: MIZKAN GROUP CORPORATION, MIZKAN CO., LTDInventor: Hiroshi Takemura
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Publication number: 20100291052Abstract: Designed to afford a novel microorganism promoting gastric juice secretion and having platelet increasing activity, and to afford a pharmaceutical agent composed of a product secreted by the novel microorganism. The novel microorganism, international deposit number: NITE BP-295, belongs to the species Bacillus pumilus, and is characterized by taking either form of coccus and bacillus, and makes figure-eight movement. The novel microorganism of the present invention is the novel microorganism having the gene represented by SEQ ID No: 1, and an object of the present invention is to afford the microorganism and a gastric juice secretion-promoting composition composed of the product secreted by the novel microorganism.Type: ApplicationFiled: August 10, 2007Publication date: November 18, 2010Inventor: Yoshichika Kameyama
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Publication number: 20100284981Abstract: An isolated Bacillus strain LSSAO1 is provided. When fed to a bird, this and other Bacillus strains described herein provide benefits to the birds. For example, administration of the one or more Bacillus strain can increase low G+C, gram positive bacteria in the gastrointestinal flora of the bird. These type of bacteria are increased by antibiotics and include beneficial Clostridium. Administration of the one or more Bacillus strain can also inhibit pathogen in the bird, such as E. coli, Salmonella, and Clostridium. These benefits can enhance feed conversion in poultry. Useful combinations of Bacillus strains and methods of using one or more Bacillus strain are also provided.Type: ApplicationFiled: July 1, 2010Publication date: November 11, 2010Applicant: DANISCO A/SInventors: Tammy Baltzley, Firmin Lago, Tony Neumann, Thomas Rehberger, Shelly Gebert
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Publication number: 20100285562Abstract: A non-reducing saccharide by providing a novel non-reducing saccharide composed of glucose as constituents, a novel enzyme forming the non-reducing saccharide, a method and process for producing the same, a DNA encoding the enzyme, a recombinant DNA and transformant comprising the DNA, a composition comprising the non-reducing saccharide, and uses thereof are provided by use of an isocyclomaltooligosaccharide(s) having a structure represented by Formula 1, Cyclo{?6)-[?-D-Glcp-(1?4)]n-?-D-Glcp-(1?}, wherein “n” is 4 or 5.Type: ApplicationFiled: April 21, 2010Publication date: November 11, 2010Applicant: KABUSHIKI KAISHA HAYASHIBARA SEIBUTSU KAGAKU KENKYUJOInventors: Hikaru Watanabe, Tomoyuki Nishimoto, Michio Kubota, Shigeharu Fukuda, Toshio Miyake
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Publication number: 20100279388Abstract: It is intended to provide an agent for treating fungi which is capable of controlling the production cost while using a microorganism with high safety, and also maintaining an adhesion state on a surface of a target for a predetermined period of time or longer during use, and a method for treating fungi. The agent for treating fungi is characterized by containing a microorganism-containing liquid obtained by adding water to powder prepared by mixing a Bacillus sphaericus microorganism, a Bacillus subtilis microorganism, or a Bacillus thuringiensis microorganism with livestock manure treated at a high temperature between 60° C. and 150° C., a surfactant, and a propellant. The agent for treating fungi is characterized in that, preferably, the surfactant is at least one type selected from the group consisting of sodium lauryl sulfate, polyoxyethylene cetyl ether, and potassium fatty acid, and the propellant is LP gas.Type: ApplicationFiled: September 11, 2008Publication date: November 4, 2010Applicant: Big Bio Co., Ltd.Inventors: Keiko Sakamoto, Toshiaki Iwashita
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Patent number: 7824882Abstract: Provided is a test system, a test method and a test kit for the determination of the presence of an antibiotic in a fluid based on a test medium comprising a test organism, at least one substance that provides a solid state, nutrients and an indicator, wherein the end-pH of said test medium at or after the time required for said determination is equal to or higher than 7.2 Said pH value can be realized by adding oligosaccharides, preferably a disaccharide or trisaccharide (eg. Lactose, gentobiose, maltotriose). Most preferably, said oligosaccharide is a non-reducing oligosaccharide (eg. Raffinose, sucrose or trehalose).Type: GrantFiled: June 2, 2005Date of Patent: November 2, 2010Assignee: DSM IP Assets B.V.Inventors: Pieter Cornelis Langeveld, Johannes Theodorus Arie van Pelt, Jacobus Stark, Tim de Graaf
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Patent number: 7824894Abstract: Fructooligosaccharide (FOS)-related protein nucleic acid molecules and polypeptides and fragments and variants thereof are disclosed in the current invention. In addition, FOS-related fusion proteins, antigenic peptides, and anti-FOS-related antibodies are encompassed. The invention also provides recombinant expression vectors containing a nucleic acid molecule of the invention and host cells into which the expression vectors have been introduced. Methods for producing the polypeptides of the invention and methods for their use are further disclosed.Type: GrantFiled: July 2, 2008Date of Patent: November 2, 2010Assignee: North Carolina State UniversityInventors: Rodolphe Barrangou, Todd R. Klaenhammer, Eric Altermann
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Publication number: 20100267094Abstract: The present invention provides a bacterium which has an ability to produce a useful metabolite derived from acetyl-coenzyme A, such as L-glutamic acid, L-glutamine, L-proline, L-arginine, L-leucine, L-cysteine, succinate, and polyhydroxybutyrate, wherein said bacterium is modified so that activities of D-xylulose-5-phosphate phosphoketolase and/or fructose-6-phosphate phosphoketolase are enhanced. The present invention also provides a method for producing the useful metabolite using the bacterium.Type: ApplicationFiled: June 28, 2010Publication date: October 21, 2010Inventors: Yury Ivanovich Kozlov, Akito Chinen, Hiroshi Izui, Yoshihiko Hara, Hisashi Yasueda, Konstantin Vyacheslavovich Rybak, Ekaterina Aleksandrovna Slivinskaya, Joanna Yosifovna Katashkina
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Publication number: 20100247706Abstract: A bacillus composition characterized by fast germination and outgrowth in bile salts (simulated gut environment) and by high-level secretion of phytase. The bacillus composition may be used as supplement in animal feed where it has a probiotic (health promoting) effect and increases the digestion and availability of nutrients from animal feeds.Type: ApplicationFiled: June 11, 2008Publication date: September 30, 2010Inventors: Inge Knap, Ane Knarreborg, Thomas Dyrmann Leser, Bente Lund
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Publication number: 20100248369Abstract: A renal function-ameliorating agent and a serum creatinine-lowering agent, each comprising as an active ingredient a culture of a bacterium belonging to the genus Bacillus, are disclosed. The bacterium belonging to the genus Bacillus is preferably Bacillus subtilis, and more preferably Bacillus subtilis C-3102 (FERM BP-1096). The renal function-ameliorating agent and the serum creatinine-lowering agent are useful for treating and preventing diseases such as arteriosclerosis.Type: ApplicationFiled: August 14, 2007Publication date: September 30, 2010Applicant: CALPIS CO., LTD.Inventors: Hiromi Suzuki, Shigeru Fujiwara
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Patent number: 7803602Abstract: The present invention is directed to methods for producing and selecting new mutant strains of B. fragilis that constitutively express a particular capsular polysaccharide or only selected capsular polysaccharides; compositions directed to the new mutant strains of B. fragilis that constitutively express a particular capsular polysaccharide or only selected capsular polysaccharides; improved methods for purification of individual capsular polysaccharides; and compositions directed to new res02 and inv19 genes and their gene products. Significantly, the present invention provides methods and compositions for overexpressing and purifying immunomodulatory capsular polysaccharide A (PSA) in high yield.Type: GrantFiled: August 20, 2007Date of Patent: September 28, 2010Assignee: The Brigham and Woman's Hospital, Inc.Inventors: Laurie E. Comstock, Katja G. Weinacht, Michael J. Coyne, Dennis L. Kasper, Arthur O. Tzianabos
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Publication number: 20100240577Abstract: Novel bacteriocins produced by novel bacterial strains are used for at least reducing the levels of colonization by at least one target bacteria in animals, especially poultry.Type: ApplicationFiled: March 19, 2010Publication date: September 23, 2010Inventors: Norman J. Stern, Edward A. Svetoch, Nikolay N. Urakov, Boris V. Eruslanov, Larisa I Volodina, Yuri N. Kovalev, Tamara Y. Kudryavtseva, Vladimir V. Perelygin, Victor D. Pokhilenko, Vladimir P. Levchuk, Valery N. Borzenkov, Olga E. Svetoch, Eugeni V. Mitsevich, Irina P. Mitsevich
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Publication number: 20100210000Abstract: The present application relates to probiotic grain-based compositions comprising lactic acid-producing bacteria.Type: ApplicationFiled: October 16, 2009Publication date: August 19, 2010Applicant: Ganeden Biotech, Inc.Inventors: Sean Farmer, Andrew R. Lefkowitz, Michael Bush, David Maske
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Publication number: 20100209406Abstract: Bacillus strains that inhibit pathogenic swine E. coli and/or improve performance are provided. Inhibition of pathogenic swine E. coli decreases E. coli disease. At least one strain enhanced swine performance by improving average daily gain, feed efficiency, and feed intake. Preferred Bacillus strains are of species that are included on the GRAS list. Bacillus species are sporeformers and therefore are highly stable and can be fed to swine.Type: ApplicationFiled: October 5, 2009Publication date: August 19, 2010Inventors: Thomas G. Rehberger, Dorrie Sue Jordan-Parrott
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Patent number: 7767203Abstract: The invention relates generally to digestive disorders, and in particular to methods for treating irritable bowel syndrome by increasing carbohydrate absorption by administering a composition containing a Bacillus coagulans bacterium.Type: GrantFiled: August 9, 2004Date of Patent: August 3, 2010Assignee: Ganeden Biotech, Inc.Inventors: Sean Farmer, Andrew R. Lefkowitz
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Publication number: 20100179060Abstract: The invention relates to a new fungicide product for the biological control of phytopathogenic fungi, which contains a new isolate of Bacillus velezensis strain AH2, deposited in the Spanish Type Culture Collection (CECT) as CECT-7221, which has a high antagonist activity against this type of pathogen, as well as the property of stimulating plant growth by different mechanisms. The invention also relates to a liquid formulation which has good stability at ambient temperature and is effective in the treatment of diseases caused by phytopathogenic fungi and for the stimulation of plant growth.Type: ApplicationFiled: February 27, 2008Publication date: July 15, 2010Applicant: PROBELTE, S.A.Inventors: Ana Isabel Fernandez Martinez, Mario Jorge Villaverde Fernandez, Juan Antonio Casanova Roca, Jorge Malo Lopez-Roman, Jose Antonio Nicolas Martinez, Isidro Blanca Pico
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Patent number: 7754469Abstract: An isolated Bacillus strain LSSAO1 is provided. When fed to a bird, this and other Bacillus strains described herein provide benefits to the birds. For example, administration of the one or more Bacillus strain can increase low G+C, gram positive bacteria in the gastrointestinal flora of the bird. These type of bacteria are increased by antibiotics and include beneficial Clostridium. Administration of the one or more Bacillus strain can also inhibit pathogen in the bird, such as E. coli, Salmonella, and Clostridium. These benefits can enhance feed conversion in poultry. Useful combinations of Bacillus strains and methods of using one or more Bacillus strain are also provided.Type: GrantFiled: November 30, 2006Date of Patent: July 13, 2010Assignee: Agtech Products, IncInventors: Tammy Baltzley, Firmin Lago, Tony Neumann, Thomas Rehberger, Shelly Gebert
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Patent number: 7754454Abstract: A process for producing a sulfur-containing ?-hydroxycarboxylic acid compound, which comprises a step for reacting a sulfur-containing dihydroxy compound with a cell or a material from treated cell of a microorganism that has an ability to convert the sulfur-containing dihydroxy compound to a corresponding ?-hydroxycarboxylic acid compound.Type: GrantFiled: September 2, 2005Date of Patent: July 13, 2010Assignee: Sumitomo Chemical Co., Ltd.Inventors: Hiroyuki Asako, Koji Hagiya
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Publication number: 20100172873Abstract: Described are strains including Enterococcus faecium strain 8G-1 (NRRL B-50173), Enterococcus faecium strain 8G-73 (NRRL B-50172), Bacillus pumilus strain 8G-134 (NRRL B-50174) and strains having all of the identifying characteristics of each of these strains. One or more of the strains can be used to treat or prevent acidosis. They can also be used to improve other measures of ruminant health and/or performance. Methods of using the strains, alone and in combination, are described. Methods of making the strains are also provided.Type: ApplicationFiled: December 2, 2009Publication date: July 8, 2010Applicants: DANISCO A/S, LALLEMAND, INC.Inventors: Keith J. Mertz, Thomas G. Rehberger
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Patent number: 7745115Abstract: Provided is a method of surveillance for hazardous materials, e.g., chemical, biological and radiological agents. The method comprises assaying a sample derived from materials collected from a sample domain for the presence of a chemical, biological, or radiological agent. The sample domain comprises at least one collection point from which the materials are collected in a pre-existing operation unrelated to surveillance.Type: GrantFiled: April 1, 2004Date of Patent: June 29, 2010Assignee: New York UniversityInventors: Page W. Caufield, Deepak Saxena, Michael C. Alfano
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Patent number: 7736588Abstract: Detection arrangement and method for detecting presence of a residue in a sample include determining color values of the sample, associated with the L*a*b color model. A value of a composite parameter Z is calculated as follows: A=wL+waa+wbb where wL, wa and wb are weighting factors having a value depending on the residue and the sample. A determination is made whether or not the sample comprises more or less than a predetermined amount of the residue in dependence on the value of the composition parameter Z. In a preferred embodiment, the arrangement is used to detect antibiotic residues, e.g. penicillin-G, in food products, elg. Milk, or body fluids, e.g. blood, urine.Type: GrantFiled: October 10, 2002Date of Patent: June 15, 2010Assignee: DSM IP Assets B.V.Inventors: Pieter Cornelis Langeveld, Karl Heinrich Van Hemert, Johannes Hendrik Pieter Machiel Kerkhof, Jacobus Stark
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Publication number: 20100143316Abstract: An isolated Bacillus amyloliquefaciens Ba-BPD1 having an Accession No. of DSM 21836 is provided. This novel strain has unique 16S ribosomal RNA sequenced as SEQ ID NO:1 and produces amylase, protease, cellulase and lipase, fibrinolytic enzyme to show their biodegradation capacities. Further, B. amyloliquefaciens Ba-BPD1 produces the antibiotic substances, such as iturin, fengycin and surfactin, and has antimicrobial capacity for inhibiting the fungal or bacterial growth. In conclusion, the novel strain of Bacillus amyloliquefaciens Ba-BPD1 and its products can be applied in agriculture, wastewater treatment, food industry and chemical industry.Type: ApplicationFiled: December 5, 2008Publication date: June 10, 2010Applicants: Council of Agriculture, Executive YuanInventors: Feng-Chia Hsieh, Suey-Sheng Kao
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Publication number: 20100136641Abstract: Bacteria that are not natural butanol producers were found to have increased tolerance to butanol when the membrane content of unsaturated trans fatty acids was increased. Feeding cells with unsaturated trans fatty acids increased their concentration in the membrane, which may also be accomplished by expressing a fatty acid cistrans isomerase.Type: ApplicationFiled: November 25, 2009Publication date: June 3, 2010Applicant: BUTAMAX(TM) ADVANCED BIOFUELS LLCInventors: DENNIS FLINT, TINA K. VAN DYK
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Publication number: 20100129333Abstract: Disclosed is an anti-fatty liver agent comprising a cell and/or a culture obtained by culturing a lactic acid bacterium belonging to Latobacillus gasserti as an active ingredient. Also disclosed is a pharmaceutical agent having an anti-fatty liver activity or an anti-fatty liver beverage/food comprising a cell and/or a culture obtained by culturing a lactic acid bacterium belonging to Latobacillus gasserti as an active ingredient.Type: ApplicationFiled: July 24, 2007Publication date: May 27, 2010Applicant: Snow Brand Milk Products, Co., Ltd.Inventors: Hiroshi Kawakami, Hiroaki Matsuyama, Masao Sato, Katsumi Imaizumi
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Patent number: 7718407Abstract: A process for the preparation of vitamin K2 (MK-7) comprising the culture of Bacillus subtilis mutant strain GN13/72-DSM 17766 deposited on Dec. 5, 2005 at the DSMZ.Type: GrantFiled: December 8, 2006Date of Patent: May 18, 2010Assignee: GNOSIS S.p.A.Inventors: Alberto Benedetti, Simona Daly, Roberto Xaiz, Hermes Pagani
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Patent number: 7718404Abstract: The object of the present invention is to provide an ?-isomaltosylglucosaccharide-forming enzyme, process of the same, cyclotetrasaccharide, and saccharide composition comprising the saccharide which are obtainable by using the enzyme; and is solved by establishing an ?-isomaltosylglucosaccharide-forming enzyme which forms a saccharide, having a glucose polymerization degree of at least three and having both the ?-1,6 glucosidic linkage as a linkage at the non-reducing end and the ?-1,4 glucosidic linkage other than the linkage at the non-reducing end, by catalyzing the ?-glucosyl-transfer from a saccharide having a glucose polymerization degree of at least two and having the ?-1,4 glucosidic linkage as a linkage at the non-reducing end without substantially increasing the reducing power; ?-isomaltosyl-transferring method using the enzyme; method for forming ?-isomaltosylglucosaccharide; process for producing a cyclotetrasaccharide having the structure of cyclo{66)-?-D-glucopyranosyl-(163)-?-D-glucopyranosyl-Type: GrantFiled: May 9, 2007Date of Patent: May 18, 2010Assignee: Kabushiki Kaisha Hayashibara Seibutsu Kagaku KenkyujoInventors: Michio Kubota, Keiji Tsusaki, Takanobu Higashiyama, Shigeharu Fukuda, Toshio Miyake
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Patent number: 7718415Abstract: Acetyl esterase producing bacterial strains or functional mutants thereof and methods of using acetyl esterase producing bacterial strains as forage additives are disclosed.Type: GrantFiled: February 22, 2008Date of Patent: May 18, 2010Assignee: Pioneer Hi-Bred International, Inc.Inventors: Victor Nsereko, Christine Dengler, Brenda Smiley
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Publication number: 20100120623Abstract: The invention relates to a method of indirectly identifying a polynucleotide comprising an open reading frame having an improved expression level from a library of polynucleotides encoding the same polypeptide of interest, or variants or homologues thereof, in a host cell expression library, the method comprising the steps of: a) providing an expression library in a host cell, said library comprising at least two different open reading frames encoding the same polypeptide of interest, or variants or homologues thereof, each open reading frame being translationally coupled to a downstream gene encoding a screenable or selectable reporter; b) culturing the host cell under conditions conducive to the expression of the polypeptide of interest, or variant or homologue thereof; and c) screening and/or selecting for a host cell having an improved expression level of the translationally coupled reporter when compared to one or more other host cells of the library, thus indirectly identifying a polynucleotide comprisiType: ApplicationFiled: December 20, 2007Publication date: May 13, 2010Applicant: Novozymes A/SInventor: Steen Troels Jorgensen
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Patent number: 7713726Abstract: Compositions containing a lactic acid-producing bacterial strain, e.g., Bacillus coagulans for inhibition of pathogenic bacterial infections. Spores or extracellular products produced by the bacterial strains are also useful as inhibitory agents.Type: GrantFiled: February 13, 2007Date of Patent: May 11, 2010Assignee: Ganeden Biotech IncorporatedInventor: Sean Farmer
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Publication number: 20100112670Abstract: Embodiments of the present invention relates to the incorporation and use of a regulated genetic suicide mechanism for use in the improved purification of biologics, including adjunct use in various eubacterial minicell production and purification methodologies. Described herein are high-yield eubacterial minicell-producing strains with genetic modifications that comprise a regulated genetic suicide mechanism that irreparably destroys the parent cell chromosome such that live parental cells in a culture can be functionally eliminated at any time during the course of a minicell production and purification run. Embodiments of the present invention also describe methods useful in the elimination of live parental cells during the production of other cell-based biologics.Type: ApplicationFiled: June 23, 2009Publication date: May 6, 2010Applicant: Vaxiion Therapeutics, Inc.Inventors: Matthew J. Giacalone, Stanley Maloy, Shingo Tsuji
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Publication number: 20100098674Abstract: The present invention relates to the use of isolated polypeptides as a coccidiostat and/or as a histomonastat. An example of a polypeptide of the invention is the so-called L12 protein from Bacillus licheniformis ATCC 14580. The invention furthermore relates to the probiotic use of strains of Bacillus, which produce proteins related to L12.Type: ApplicationFiled: December 17, 2007Publication date: April 22, 2010Inventors: Gilbert Weber, Jiri Broz
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Patent number: 7700094Abstract: Acetyl esterase producing bacterial strains or functional mutants thereof and methods of using acetyl esterase producing bacterial strains as forage additives are disclosed.Type: GrantFiled: February 22, 2008Date of Patent: April 20, 2010Assignee: Pioneer Hi-Bred International, Inc.Inventors: Victor Nsereko, Christine Dengler, Brenda Smiley
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Publication number: 20100093037Abstract: The present invention provides novel methods of growing of microorganisms in cell culture media comprising soy components (e.g., soy molasses) as a carbon source. The present invention further provides novel cell culture media comprising soy components (e.g., soy molasses) as a carbon source. In certain embodiments, inventive cell culture media substantially lack a carbon source other than soy molasses (e.g., the media substantially lack glucose and glycerol). In certain embodiments, inventive cell culture media comprise soy components (e.g., soy molasses) as the sole carbon source.Type: ApplicationFiled: September 23, 2009Publication date: April 15, 2010Inventors: KEVIN A. JARRELL, GABRIEL REZNIK, MICHELLE A. PYNN
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Publication number: 20100092613Abstract: The present invention relates to a novel phytase enzyme, a novel isolated nucleic acid molecule coding the enzyme, and a novel Yersinia intermedia having phytase activity. Particularly, the present invention relates to the phytase having (a) Theoretical molecular weight 45.5 kDa, (b) high specific activity 3960±248 U/mg, (c) high stability at high temperature and wide pH, (d) optimal pH of 4.0-5.0, (e) optimal temperature of 50-60° C., (f) high resistance to pepsin and trypsin. The phytase is very suitable to be used in feed of monogastrics as feed additive. The present invention also relates to a recombinant vector comprising said nucleic acid molecule, a recombinant host cell (e.g., Pichia pastoris) harboring said recombinant vector, and a method for producing phytase using the recombinant host cell. The present invention further provides a feed additive comprising said phytase and/or host cells expressing a phytase as effective ingredient.Type: ApplicationFiled: April 30, 2006Publication date: April 15, 2010Inventors: Bin Yao, Huiying Luo, Huoqing Huang, Yaru Wang, Tiezheng Yuan, Xiuyun Shi, Yingguo Bai, Kun Meng, Peilong Yang
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Publication number: 20100093060Abstract: The present invention provides novel methods of growing of microorganisms in cell culture media comprising cellulosic material as a carbon source. The present invention further provides novel cell culture media cellulosic material as a carbon source. In certain embodiments, inventive cell culture media substantially lack a carbon source other than cellulosic material (e.g., the media substantially lack glucose and glycerol). In certain embodiments, inventive cell culture media comprise cellulosic material as the sole carbon source.Type: ApplicationFiled: September 23, 2009Publication date: April 15, 2010Inventors: Kevin A. Jarrell, Gabriel Reznik, Michelle A. Pynn, Joy D. Sitnik
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Publication number: 20100087516Abstract: The present invention relates to uses of Macrolactin A produced by Bacillus polyfermenticus KJS-2 (KCCM 10769P), which is a new bacillus strain, as an antibiotic. Macrolactin A of the present invention, which is produced by Bacillus polyfermenticus KJS-2, shows a broad spectrum of antibiotic activity against a variety of microorganisms and fungi, and is proved to be very efficient for the inhibition of particularly vancomycin-resistant enterococci (VRE) and methicillin-resistant Staphylococcus Aureus (MRSA) that are multidrug-resistant bacteria. The antibiotic Macrolactin A produced by Bacillus polyfermenticus KJS-2, can be used as an excellent antibiotic against vancomycin-resistant enterococci (VRE) and methicillin-resistant Staphylococcus Aureus (MRSA), and thus the present invention is a very useful invention for medical industry.Type: ApplicationFiled: March 12, 2008Publication date: April 8, 2010Inventors: Jae-Seon Kang, Chun-Gyu Kim, Dong-Hee Kim, Kang-Min Kim, Dong-Hun Kim, Jin-Young Lee, Guang-Jin Choi, In-June Cha, Jae-Sun Hong, Yong-Geun Hong
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Publication number: 20100086978Abstract: The invention features methods for producing isoprene from cultured cells using a feedback-resistant mevalonate kinase polypeptide, such as an archaeal mevalonate kinase polypeptide. The resulting isoprene compositions may have increased yields and/or purity of isoprene.Type: ApplicationFiled: September 15, 2009Publication date: April 8, 2010Inventors: Zachary Q. Beck, Anthony R. Calabria, Michael C. Miller, Dmitrii V. Vaviline
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Patent number: 7691620Abstract: Production of ethanol in a thermophilic bacterium can be achieved by transformation of the bacterium with a heterologous gene encoding pyruvate decarboxylase. The bacterium comprises native alcohol dehydrogenase function and does not comprise a gene encoding a functional lactate dehydrogenase. The bacterium may be grown at elevated temperatures to allow the ethanol to be conveniently removed in a vaporized form from the fermentation medium. Traditional mesophilic microorganisms are incapable of growth at the envisioned elevated temperatures.Type: GrantFiled: March 13, 2007Date of Patent: April 6, 2010Assignee: Elsworth Biotechnology LimitedInventors: Edward Green, Muhammad Javed, Namdar Baghaei-Yazdi
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Publication number: 20100075376Abstract: The present invention relates to cells producing at least one polypeptide of interest and expressing one or more recombinant nuclease encoding gene(s) thereby producing the nuclease(s), and methods for producing a polypeptide of interest essentially free from contaminating DNA, said method comprising the steps of: (a) cultivating a cell that produces at least one polypeptide of interest and expresses one or more recombinant nuclease encoding gene(s) thereby producing the nuclease(s); and (b) isolating the polypeptide of interest.Type: ApplicationFiled: November 30, 2007Publication date: March 25, 2010Applicant: NOVOZYMES A/SInventors: Michael Dolberg Rasmussen, Jon Martin Persson