Escherichia (e.g., E. Coli, Etc.) Or Salmonella Patents (Class 435/252.8)
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Patent number: 7785879Abstract: The present invention relates, inter alia, to PXR polypeptides and crystals that are useful, for example, for crystallization and in assays for identification of modulators of PXR.Type: GrantFiled: September 18, 2006Date of Patent: August 31, 2010Assignee: Schering CorporationInventors: Wenyan Wang, Shahriar Shane Taremi, Winifred W. Prosise, Paul Reichert, Charles A. Lesburg, Vincent S. Madison, Kuo-Chi Cheng
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Patent number: 7776566Abstract: A microorganism which has a gene encoding an enzyme in which feedback inhibition is desensitized by substitution of one or two amino acids in PRPP amidotransferase encoded by purF of Escherichia coli, a gene encoding a protein which is an inactivated repressor of purine nucleotide biosynthesis encoded by purR, a gene encoding an enzyme which is inactivated purine nucleoside phosphorylase encoded by deoD, a gene encoding an enzyme which is inactivated succinyl-AMP synthase encoded by purA, a gene encoding an enzyme which is inactivated 6-phosphogluconate dehydrase encoded by edd, a gene encoding an enzyme which is inactivated phosphoglucose isomerase encoded by pgi and like is bred and a purine nucleoside is produced by culturing the microorganism.Type: GrantFiled: March 5, 2007Date of Patent: August 17, 2010Assignee: Ajinomoto Co., Inc.Inventors: Hiroshi Matsui, Hisashi Kawasaki, Megumi Shimaoka, Yasuhiro Takenaka, Osamu Kurahashi
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Publication number: 20100190653Abstract: The invention relates to an isolated polynucleotide having a polynucleotide sequence which codes for the alr gene, and a host-vector system having a coryneform host bacterium in which the alr gene is present in attenuated form and a vector which carries at least the alr gene according to SEQ ID No 1, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.Type: ApplicationFiled: November 27, 2002Publication date: July 29, 2010Inventors: Andreas Tauch, Michael Binder, Walter Pfefferle, Georg Thierbach, Jorn Kalinowski, Alfred Puhler
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Publication number: 20100183649Abstract: A method for producing picornaviral capsid protein complexes (e.g., picornavirus like particles) in E. coli using a small-ubiquitin-related fusion protein expression system and an E. coli strain used in practicing this method. Also disclosed is use of the picornaviral capsid protein complexes like thus prepared for eliciting immune responses.Type: ApplicationFiled: January 7, 2010Publication date: July 22, 2010Applicant: Academia SinicaInventors: Ting-Fang Wang, Shu-Mei Liang
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Publication number: 20100184164Abstract: The present invention relates to a method for producing L-methionine and organic acid comprising the following steps: Step 1) preparing a strain producing L-methionine precursor and producing L-methionine precursor by the fermentation of the strain; Step 2) producing L-methionine and organic acid by the enzyme reaction process with the L-methionine precursor as a substrate, and microorganism strains used in each step.Type: ApplicationFiled: July 30, 2007Publication date: July 22, 2010Applicant: CJ CHEILJEDANG CORPORATIONInventors: So-young Kim, Kwang-myung Cho, Yong-uk Shin, Hye-won Um, Kyung-oh Choi, Jin-sook Chang, Young-wook Cho, Young-hoon Park
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Publication number: 20100183549Abstract: The invention is directed compositions and methods related to bacterial cells physically associated with ceramide-like glycolipids. The invention allows for delivery of ceramide-like glycolipid adjuvants directly to the same cells that become infected with a bacterial vaccine. The compositions and methods of the present invention are useful for the prevention and treatment of diseases.Type: ApplicationFiled: January 8, 2010Publication date: July 22, 2010Applicant: Albert Einstein College of Medicine of Yeshiva UniversityInventors: Steven A. Porcelli, Manjunatha M. Venkataswamy
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Patent number: 7758855Abstract: The invention is related to intracellularly induced bacterial DNA promoters and vaccines against Bacillus anthracis.Type: GrantFiled: March 17, 2006Date of Patent: July 20, 2010Assignee: The United States of America as represented by the Department of Health and Human ServicesInventors: Dennis J. Kopecko, Manuel Osorio, Siba Bhattacharyya, Chandrakant P. Giri, Milan Blake
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Patent number: 7749755Abstract: Novel polypeptides, designated Apo-2, which are capable of modulating apoptosis are provided. Compositions including Apo-2 chimeras, nucleic acid encoding Apo-2, and antibodies to Apo-2 are also provided.Type: GrantFiled: December 9, 2005Date of Patent: July 6, 2010Assignee: Genentech, Inc.Inventor: Avi J. Ashkenazi
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Publication number: 20100167379Abstract: Disclosed are novel bacterial hosts that are capable of high efficiency transformation with methylated and/or unmethylated nucleic acids, and that are bacteriophage resistant. Such bacteria contain: (1) an F? episome that confers high efficiency transformability; (2) one or more mutations that allow transformation of methylated nucleic acids; (3) one or more mutations that allow transformation with unmethylated nucleic acids; and/or (4) one or more mutations that confer resistance to bacteriophage infection. Also disclosed are methods for transforming such bacteria, and kits that contain such bacteria (e.g., that have been made competent for transformation).Type: ApplicationFiled: December 23, 2009Publication date: July 1, 2010Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Fredric R. Bloom, Brian Schmidt
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Patent number: 7741089Abstract: The invention provides laccases, polynucleotides encoding these enzymes, the use of such polynucleotides and polypeptides. In one aspect, the invention relates to the enzymatic production of nootkatone by way of the conversion of valencene using proteins having a laccase activity, e.g., a novel laccase of the invention. In one aspect, the invention provides methods of depolymerizing lignin, e.g., in a pulp or paper manufacturing process, using a polypeptide of the invention. In another aspect, the invention provides methods for oxidizing products that can be mediators of laccase-catalyzed oxidation reactions, e.g., 2,2-azinobis-(3-ethylbenzthiazoline-6-sulfonate) (ABTS), 1-hydroxybenzotriazole (HBT), 2,2,6,6-tetramethylpiperidin-1-yloxy (TEMPO), dimethoxyphenol, and the like.Type: GrantFiled: August 11, 2004Date of Patent: June 22, 2010Assignee: Verenium CorporationInventors: Tim Hitchman, Dan E. Robertson, Masao Hiraiwa, Yoko Phillips, Kevin A. Gray
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Publication number: 20100150965Abstract: The invention is related to intracellularly induced bacterial DNA promoters and vaccines against Bacillus anthracis.Type: ApplicationFiled: March 17, 2006Publication date: June 17, 2010Inventors: Dennis J. Kopecko, Manuel Osorio, Siba Bhattacharyya, Chandrakant P. Giri, Milan Blake
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Patent number: 7736899Abstract: The present invention relates to a method for providing bacterial or yeast cells with the capacity to produce a protein, the amino acid sequence of which comprises at least one unconventional amino acid. The method involves (a) introducing at least one missense mutation in a target codon of a gene encoding a protein required for the growth of the bacterial or yeast cells, where the mutated protein synthesized from the mutated gene is not functional in the bacterial or yeast cells. The method also involves (b) selecting the bacterial or yeast cells obtained in (a) in a culture medium which (1) does not contain a nutrient compensating for the loss of functionality of the mutated protein and (2) contains an unconventional amino acid which restores the functionality of the protein required for growth of the bacterial or yeast cells, in which the unconventional amino acid is that encoded by the target codon.Type: GrantFiled: October 28, 1999Date of Patent: June 15, 2010Assignee: Institut PasteurInventors: Philippe Marliere, Volker Doring, Henning Mootz
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Publication number: 20100143982Abstract: The present invention provides a method for producing an L-amino acid using a bacterium of the Enterobacteriaceae family, particularly a bacterium belonging to the genus Escherichia or Pantoea, which has been modified to attenuate expression of the aldH gene.Type: ApplicationFiled: May 23, 2008Publication date: June 10, 2010Inventors: Dmitriy Vladimirovich Filippov, Elvira Borisovna Voroshilova, Tatyana Viktorovna Leonova, Mikhail Markovich Gusyatiner
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Publication number: 20100143433Abstract: The present invention relates to reindeer bone formation inducing protein called bone morphogenetic protein (BMP), such as BMP-6, containing a heparin binding site and nucleotide molecules encoding the proteins and host cells expressing the proteins. The present invention relates also to the use of the bone morphogenetic protein for treating disorders related to bone and cartilage formation. The present invention further relates to osteogenic devices and pharmaceutical compositions containing the protein.Type: ApplicationFiled: May 26, 2006Publication date: June 10, 2010Inventors: Elli Birr, Mari Ulmanen, Oili Hietala, Marja Juustila, Heli Korkala, Pekka Jalovaara
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Publication number: 20100136641Abstract: Bacteria that are not natural butanol producers were found to have increased tolerance to butanol when the membrane content of unsaturated trans fatty acids was increased. Feeding cells with unsaturated trans fatty acids increased their concentration in the membrane, which may also be accomplished by expressing a fatty acid cistrans isomerase.Type: ApplicationFiled: November 25, 2009Publication date: June 3, 2010Applicant: BUTAMAX(TM) ADVANCED BIOFUELS LLCInventors: DENNIS FLINT, TINA K. VAN DYK
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Publication number: 20100135973Abstract: The present invention provides a biologically pure isolate of the genus Salmonella having a disruption of at least one gene selected from the group consisting of aroA, rfaH, and thyA, as well as a method of treating cancer including the step of administering such a Salmonella to a subject in need thereof.Type: ApplicationFiled: May 26, 2009Publication date: June 3, 2010Inventors: Abraham Eisenstark, Robert A. Kazmierczak
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Publication number: 20100129883Abstract: Biological method for conversion of a lignocellulosic hydrolysate into a desired biochemical product. Use of a plurality of substrate-selective cells allows different sugars in a complex mixture to be consumed concurrently and independently. The method can be readily extended to remove inhibitory compounds from hydrolysate.Type: ApplicationFiled: October 7, 2009Publication date: May 27, 2010Inventors: Mark A. Eiteman, Elliot Altman
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Publication number: 20100124759Abstract: The present invention relates generally to the use of droplets to culture and/or assay cells or other species. In some cases, the cells or other species may be sorted based upon the results of the culture and/or assay. In some embodiments, cells other species can be encapsulated in droplets and exposed to one or more agents (e.g., a sugar, an indicator dye, etc.). For instance, in some cases, exposure of cells to the agents may result in the production of metabolites or other compounds (e.g., amino acids, proteins, organic acids, etc.) which may be, for example, assayed or otherwise determined. In some embodiments, the reaction of an agent with cells and/or other species within a droplet may reveal a property of the cells or other species (e.g., sugar consumption, growth rate, ability to withstand exposure to the agent, etc.). As an example, cells that produce desired metabolites or exhibit certain properties may be separated from the other cells via sorting techniques.Type: ApplicationFiled: June 26, 2009Publication date: May 20, 2010Applicants: Massachusetts Institute of Technology, President and Fellows of Harvard CollegeInventors: Benjamin L. Wang, Katherine J. Humphry, David A. Weitz, Gregory Stephanopoulos
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Publication number: 20100112642Abstract: Recombinant forms of DNA sequences for CPD glycosylases, including the bacteriophage T4 gene denV, are described that are capable of expression at high levels. Active CPD glycosylases can be recovered from inclusion bodies resulting from the high expression using, for example, a homogenization process which employs stream mixing, and the active proteins can be used in, for example, topical formulations for treatment of photosensitive diseases. Stream mixing can also be used to solubilize inclusion bodies containing proteins other than CPD glycosylases.Type: ApplicationFiled: January 14, 2010Publication date: May 6, 2010Inventors: Daniel B. Yarosh, Leonard F. Estis, Elyahu Kraus
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Publication number: 20100113383Abstract: This invention provides nucleic acids and proteins involved in oligosaccharide modification in the species Bifidobacteria. The invention provides methods for utilizing the proteins of the invention to generate human milk oligosaccharides or oligosaccharide mimics. The invention also provides compositions containing the human milk oligosaccharides or oligosaccharide mimics and methods for use.Type: ApplicationFiled: September 14, 2007Publication date: May 6, 2010Applicant: The Regents of the University of CaliforniaInventors: David A. Mills, Carlito B. Lebrilla, J. Bruce German, David Sela
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Publication number: 20100112670Abstract: Embodiments of the present invention relates to the incorporation and use of a regulated genetic suicide mechanism for use in the improved purification of biologics, including adjunct use in various eubacterial minicell production and purification methodologies. Described herein are high-yield eubacterial minicell-producing strains with genetic modifications that comprise a regulated genetic suicide mechanism that irreparably destroys the parent cell chromosome such that live parental cells in a culture can be functionally eliminated at any time during the course of a minicell production and purification run. Embodiments of the present invention also describe methods useful in the elimination of live parental cells during the production of other cell-based biologics.Type: ApplicationFiled: June 23, 2009Publication date: May 6, 2010Applicant: Vaxiion Therapeutics, Inc.Inventors: Matthew J. Giacalone, Stanley Maloy, Shingo Tsuji
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Publication number: 20100105103Abstract: The present invention relates to E. coli mutants, which have enhanced alcohols tolerance and can be used in production of alcohols through fermentation. The present invention also provides a novel method to prepare the alcohol-tolerant E. coli strains.Type: ApplicationFiled: May 27, 2009Publication date: April 29, 2010Applicant: NATIONAL TAIWAN UNIVERSITYInventors: Hsueh-Fen Juan, Hirotada Mori, Hsin-Yi Chang, Hsuan-Cheng Huang, Tsui-Chin Huang, James C. Liao
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Publication number: 20100086978Abstract: The invention features methods for producing isoprene from cultured cells using a feedback-resistant mevalonate kinase polypeptide, such as an archaeal mevalonate kinase polypeptide. The resulting isoprene compositions may have increased yields and/or purity of isoprene.Type: ApplicationFiled: September 15, 2009Publication date: April 8, 2010Inventors: Zachary Q. Beck, Anthony R. Calabria, Michael C. Miller, Dmitrii V. Vaviline
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Publication number: 20100068753Abstract: Biotinylation of compounds such as peptides and peptidomimetics facilitates illicit transport of the compounds into Gram negative bacteria.Type: ApplicationFiled: September 14, 2009Publication date: March 18, 2010Applicant: University of Georgia Research Foundation, Inc.Inventors: Elliot Altman, Jennifer R. Walker
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Patent number: 7670825Abstract: The present invention provides methods of producing an isoprenoid or an isoprenoid precursor in a genetically modified host cell. The methods generally involve modulating the level of hydroxymethylglutaryl-CoA (HMG-CoA) in the cell, such that the level of HMG-CoA is not toxic to the cell and/or does not substantially inhibit cell growth, but is maintained at a level that provides for high-level production of mevalonate, IPP, and other downstream products of an isoprenoid or isoprenoid pathway, e.g., polyprenyl diphosphates and isoprenoid compounds. The present invention further provides genetically modified host cells that are suitable for use in a subject method. The present invention further provides recombinant nucleic acid constructs for use in generating a subject genetically modified host cell, including recombinant nucleic acid constructs comprising nucleotide sequences encoding one or more mevalonate pathway enzymes, and recombinant vectors (e.g., recombinant expression vectors) comprising same.Type: GrantFiled: January 17, 2007Date of Patent: March 2, 2010Assignee: The Regents of the University of CaliforniaInventors: Jay D. Keasling, Jack D. Newman, Douglas J. Pitera
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Publication number: 20100048964Abstract: The invention features methods for producing isoprene from cultured cells wherein the cells in the stationary phase. The invention also provides compositions that include these cultured cells and/or increased amount of isoprene. The invention also provides for systems that include a non-flammable concentration of isoprene in the gas phase. Additionally, the invention provides isoprene compositions, such as compositions with increased amount of isoprene or increased purity.Type: ApplicationFiled: July 1, 2009Publication date: February 25, 2010Inventors: Anthony R. CALABRIA, Marguerite A. CERVIN, Gopal K. CHOTANI, Joseph C. MCAULIFFE, Michael C. MILLER, Timothy A. SABO, Erin L. WEBSTER
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Publication number: 20100040537Abstract: The present invention encompasses prostaglandin E2 (PGE2) binding proteins. The invention relates to antibodies that are wild-type, chimeric, CDR grafted and humanized. Preferred antibodies have high affinity for prostaglandin E2 and neutralize prostaglandin E2 activity in vitro and in vivo. An antibody of the invention can be a full-length antibody, or an antigen-binding portion thereof. Methods of making and methods of using the antibodies of the invention are also provided. The antibodies, or antigen-binding portions, of the invention are useful for detecting prostaglandin E2 and for inhibiting prostaglandin E2 activity, e.g., in a human subject suffering from a disorder in which prostaglandin E2 activity is detrimental.Type: ApplicationFiled: July 8, 2009Publication date: February 18, 2010Applicant: ABBOTT LABORATORIESInventors: Jjijie Gu, Charles W. Hutchins, Rong-rong Zhu, Jianwei Shen, Maria C. Harris, Eileen Belanger, Anwar Murtaza, Edit Tarcsa, William B. Stine, Chung-ming Hsieh
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Publication number: 20100035300Abstract: A cis-TEVP fusion protein including a TEVP protease, a TEVP cleavage site and a target protein provide a platform for expression of the target protein. A trans-TEVP fusion protein including a TEVP cleavage site and a target protein, the amino-terminal portion of the target protein adjacent to the C-terminal portion of the TEVP cleavage site, the amino acidic residue in position P2 of the TEVP cleavage site being a Valine also produces the target protein by the same process. A cis-TEVP fusion protein system comprising the first fusion protein and a suitable host cell; a trans-TEVP fusion protein system comprising the second fusion protein and a suitable host cell; associated methods to produce target proteins, and kits of parts are also disclosed herein.Type: ApplicationFiled: February 27, 2006Publication date: February 11, 2010Inventors: Andrew H.-J. Wang, Ting-Fang Wang, Yan-Ping Shih, Hui-Chung Wu, Su-Ming Hu
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Publication number: 20100035313Abstract: Problem to be Solved: To provide a new microorganism capable of producing a polyhydroxyalkanoate (PHA), a PHA synthase gene, an expression cassette including the gene, a vector including the expression cassette, a transformant transformed by the vector, a polypeptide having PHA synthase activity, a method for producing a PHA synthase and a method for producing a PHA. Solution: The new microorganism is capable of producing a polyhydroxyalkanoate comprising a 16S rRNA gene whose polynucleotide sequence shows 99% or more homology to a polynucleotide sequence represented by SEQ ID No: 1, having an optimum temperature of an activity temperature range for the growth and PHA production of the microorganism of at least 45° C. and being capable of growing at a pH range from 6 to 10.Type: ApplicationFiled: July 9, 2009Publication date: February 11, 2010Inventors: Yasuharu Satou, Kenji Tajima, Masanobu Munekata, Tokuo Matsushima
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Patent number: 7659097Abstract: The present invention provides methods for a robust production of isoprenoids via one or more biosynthetic pathways. The invention also provides nucleic acids, enzymes, expression vectors, and genetically modified host cells for carrying out the subject methods. The invention also provides fermentation methods for high productivity of isoprenoids from genetically modified host cells.Type: GrantFiled: May 25, 2007Date of Patent: February 9, 2010Assignee: Amyris Biotechnologies, Inc.Inventors: Neil Stephen Renninger, Jack Newman, Keith Kinkead Reiling, Rika Regentin, Christopher John Paddon
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Publication number: 20100015212Abstract: Knockout of the meningococcal mltA homolog gives bacteria that spontaneously release vesicles that are rich in immunogenic outer membrane proteins and that can elicit cross-protective antibody responses with higher bactericidal titres than OMVs prepared by normal production processes. Thus the invention provides a bacterium having a knockout mutation of its mltA gene. The invention also provides a bacterium, wherein the bacterium: (i) has a cell wall that includes peptidoglycan; and (ii) does not express a protein having the lytic transglycosylase activity of MltA protein. The invention also provides compositions comprising vesicles that, during culture of bacteria of the invention, are released into the culture medium.Type: ApplicationFiled: October 28, 2005Publication date: January 21, 2010Applicant: CHIRON SRLInventors: Jeannette Adu-Bobie, Mariagrazia Pizza, Nathalie Norais, Germano Ferrari, Guido Grandi
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Publication number: 20100003731Abstract: The present invention provides microorganisms, in which the activity of 4-hydroxybenzoate polyprenyltransferase or 2-octaprenylphenol?2-octaprenyl-6-methoxyphenol flavin reductase is reduced or lost, and which have an ability to produce lactic acid, in particular, microorganisms comprising a chromosomal DNA in which a gene encoding a protein having 4-hydroxybenzoate polyprenyltransferase activity or a protein having 2-octaprenylphenol?2-octaprenyl-6-methoxyphenol flavin reductase activity is partially or completely defective; and a process for producing lactic acid using the microorganisms.Type: ApplicationFiled: September 5, 2006Publication date: January 7, 2010Inventors: Mikito Ito, Kimie Masuda, Hideo Mori, Makiko Kato
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Patent number: 7642069Abstract: An improved process for the production of streptokinase using a genetically engineered strain of Escherichia coli which overproduces streptokinase intracellularly and more particularly, the overall process disclosed herein, concerns with an improvement in the fermentative production of streptokinase using an optimized growth medium mainly comprised of simple salts and trace-elements; thus, in principal, the present process constitutes an improved and more economical means for the production of streptokinase which may be useful in thrombolytic therapy.Type: GrantFiled: December 9, 2005Date of Patent: January 5, 2010Assignee: Council of Scientific & Industrial ResearchInventors: Vinay Venkatrao Vyas, Govindan Rajamohan, Ramandeep, Kanak Lata Dikshit
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Publication number: 20090324551Abstract: The present invention provides for TLR agonist conjugates (compounds) and compositions, as well as methods of using them. The compounds of the invention are broad-spectrum, long-lasting, and non-toxic combination of synthetic immunostimulatory agents, which are useful for activating the immune system of a mammal, preferably a human and can help direct the pharmacophore to the receptor within the endosomes of target cells and enhance the signal transduction induced by the pharmacophore.Type: ApplicationFiled: August 21, 2006Publication date: December 31, 2009Applicant: The Regents of The University of California Office of Technology TransferInventors: Dennis A. Carson, Kenji Takabayshi, Suzanne Grimshaw, Howard B. Cottam, Michael Chan, Christina C.N. Wu
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Publication number: 20090324565Abstract: Described herein is the isolation of colicin-producing strains of E. coli for use as probiotic treatments for the prevention of E. coli K88+diarrhea. These strains of E. coli, designated as UM-17, and UM-19, express a filament and produce colicin but produce no compounds toxic to the host animal and as such inhibit the growth of E. coli K88+.Type: ApplicationFiled: May 1, 2007Publication date: December 31, 2009Applicant: UNIVERSITY OF MANITOBAInventors: Denis O. Krause, Martin Nyachoti
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Publication number: 20090325243Abstract: The present invention relates to an amino acid-producing microorganism capable of simultaneously utilizing glycerol as a carbon source, a method for preparing the microorganism, and a method for producing amino acids using the microorganism. According to the present invention, amino acids can be efficiently produced using a byproduct of biodiesel production, glycerol, thereby substituting a cheaper material for the conventional fermentation materials such as glucose.Type: ApplicationFiled: June 26, 2007Publication date: December 31, 2009Inventors: Young Hoon Park, Kwang Myung Cho, Yong Uk Shin, Hyun Ae Bae, Jin Sook Chang, Jae Yeong Ju
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Publication number: 20090318372Abstract: The present invention relates to a method for producing 2-O-glyceryl-?-D-glucopyranoside (?GG; FIG. 1) from a glucosyl donor and a glucosyl acceptor comprising the steps:—providing a sucrose phosphorylase (EC 2.4.1.7), incubating said sucrose phosphorylase with a mixture comprising a glucosyl donor and glycerol as glucosyl acceptor and isolating and/or purifying 2-O-glyceryl-?-D-glucopyranoside.Type: ApplicationFiled: September 21, 2007Publication date: December 24, 2009Applicants: Technische Universital Graz, Forschungsholding Tu Graz GMBHInventors: Christiane Gödl, Thornthan Sawangwan, Bernd Nidetzky, Mario Müller
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Publication number: 20090305368Abstract: Hydroxycarboxylic acids are produced by using a microorganism that is improved in ability to regenerate oxidized-type nicotinamide adenine dinucleotide by being provided with an enhanced NADH dehydrogenase function by introducing a gene encoding NADH dehydrogenase into a microorganism.Type: ApplicationFiled: April 27, 2007Publication date: December 10, 2009Applicant: Mitsui Chemicals Inc.Inventors: Takashi Morishige, Mitsufumi Wada, Hitoshi Takahashi, Daisuke Mochizuki, Junko Tokuda
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Publication number: 20090298127Abstract: A method is provided for producing a purine nucleoside, such as inosine and guanosine, and a method for producing a 5?-purine nucleotide such as 5?-inosinic acid or 5?-guanylic acid, using a bacterium belonging to the either genus Escherichia or genus Bacillus, wherein purine nucleoside productivity of said bacterium is enhanced by enhancing an activity of a protein encoded by the yeaS (leuE) gene.Type: ApplicationFiled: June 2, 2009Publication date: December 3, 2009Inventors: EKATERINA ALEKSANDROVNA KUTUKOVA, NATALIA PAVLOVNA ZAKATAEVA, VITALY ARKADIEVICH LIVSHITS
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Publication number: 20090286293Abstract: Non-recombinant bacteria that produce ethanol as the primary fermentation product, associated nucleic acids and polypeptides, methods for producing ethanol using the bacteria, and kits are disclosed.Type: ApplicationFiled: April 26, 2007Publication date: November 19, 2009Applicant: UNIVERSITY OF FLORIDA RESEARCH FOUNDATION, INC.Inventors: Youngnyun Kim, Keelnatham Shanmugam, Lonnie O. Ingram
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Patent number: 7618803Abstract: A method for producing L-threonine, L-valine, L-proline, L-leucine, L-methionine and L-arginine is provided using Escherichia bacteria wherein the L-amino acid productivity of the bacteria is enhanced by increasing the activity of proteins encoded by the b2682 and b2683 genes, or proteins encoded by the b1242 or b3434 gene.Type: GrantFiled: May 14, 2008Date of Patent: November 17, 2009Assignee: Ajinomoto Co., Inc.Inventors: Ekaterina Aleksandrovna Tabolina, Konstantin Vyacheslavovich Rybak, Evgeni Moiseevich Khourges, Elvira Borisovna Voroshilova, Mikhail Markovich Gusyatiner
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Patent number: 7618804Abstract: There is provided a method for producing L-threonine, L-valine, L-proline, L-leucine, L-methionine and L-arginine using a bacterium belonging to the genus Escherichia wherein the L-amino acid productivity of the bacterium is enhanced by enhancing the activities of the proteins coded by the b2682 and b2683 genes, or the protein coded by the b1242 or b3434 gene.Type: GrantFiled: May 14, 2008Date of Patent: November 17, 2009Assignee: Ajinomoto Co., Inc.Inventors: Ekaterina Aleksandrovna Tabolina, Konstantin Vyacheslavovich Rybak, Evgeni Moiseevich Khourges, Elvira Borisovna Voroshilova, Mikhail Markovich Gusyatiner
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Publication number: 20090280536Abstract: The invention provides a novel system for the tunable expression of nucleic acids encoding e.g., polypeptides such as recombinant proteins in prokaryotic systems. The system is based on the ability of T7 lysozyme (T7Lys) to inhibit the activity of T7RNAP. Expression of T7Lys can be continuously adjusted as its expression is under the control of a promoter whose activity can be titrated. The invention provides a host cell capable of expressing T7 RNA polymerase, the host cell comprising a first nucleic acid having a T7 lysozyme gene or a T7 lysozyme variant gene and a tunable promoter for controlling the expression of the T7 lysozyme gene. It also provides a host cell further comprising a second nucleic acid having a T7 promoter operably linked to a nucleic acid sequence encoding a target polypeptide, whereby expression of the target polypeptide is tuned via controlling the expression of the T7 lysozyme gene.Type: ApplicationFiled: February 27, 2009Publication date: November 12, 2009Inventors: Jan Willem DE GIER, Samuel Wagner
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Publication number: 20090275110Abstract: Provided is a microorganism that can display, on the cell surface, any molecules other than a molecule comprising amino acids, more specifically, a microorganism that displays biotin on a cell surface. The microorganism is capable of co-expressing a biotinylating enzyme and an acceptor peptide having a sequence recognized by the biotinylating enzyme, wherein the acceptor peptide is expressed on the cell surface, so that lysine of the acceptor peptide is biotinylated to display biotin on the cell surface. Also provided is a method for displaying an intended molecule, including not only a molecule comprising amino acids but also any molecules, on a cell surface of a microorganism.Type: ApplicationFiled: April 28, 2009Publication date: November 5, 2009Applicants: BIO-ENERGY CORPORATION, KANSAI CHEMICAL ENGINEERING CO., LTD.Inventors: Akihiko Kondo, Hideki Fukuda, Tsutomu Tanaka, Hideo Noda
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Publication number: 20090263416Abstract: In one aspect of the present invention, a method is provided for reducing or eliminating antibiotic resistance in bacteria, comprising exposing the bacteria to a composition comprising a yeast cell wall preparation in an amount effective for reducing or eliminating resistance of the bacteria to at least one antibiotic. In one embodiment, the method comprises exposing the bacteria to the yeast cell wall preparation in an amount effective for eliminating a plasmid conferring resistance to the antibiotic, or for preventing transfer of the plasmid between bacteria. In another aspect, a method is provided for reducing prevalence of antibiotic-resistant bacteria in an animal, comprising administering to the animal a composition comprising a yeast cell wall preparation in an amount effective for reducing or eliminating the presence of an antibiotic-resistant bacterial population in the animal.Type: ApplicationFiled: June 18, 2007Publication date: October 22, 2009Inventors: Karl A. Dawson, Melissa C. Newman
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Patent number: 7604978Abstract: The present invention relates to compositions and methods for reducing or inhibiting biofilm comprising modulating expression of a cysB gene in a cell. The invention also provides methods for modulating the expression of a cysB, cysD, cysI, cysJ, cysK, and ybiK. The invention further provides methods for identifying gene(s) involved in biofilm formation and for identifying biofilm inhibitors.Type: GrantFiled: March 21, 2005Date of Patent: October 20, 2009Assignee: Sequoia Sciences, Inc.Inventor: Gary R. Eldridge
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Publication number: 20090258399Abstract: According to the present invention, there are provided a microorganism belonging to Enterobacteriaceae wherein a function of CsrB RNA or CsrC RNA has been decreased or lost, and which has the ability to produce and accumulate an amino acid, and a process wherein the microorganism is cultured in a medium to produce and accumulate the amino acid in the culture, and the amino acid is recovered from the culture.Type: ApplicationFiled: July 25, 2007Publication date: October 15, 2009Applicant: KYOWA HAKKO BIO CO., LTDInventors: Shin-ichi Hashimoto, Koji Harada, Nozomu Kamada, Tetsuya Nishitani
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Publication number: 20090258037Abstract: Methods are provided herein for producing a vaccine, comprising culturing bacteria in microgravity. In some examples, the method includes culturing bacteria in microgravity, evaluating RNA expression, detecting an RNA that is over- or underexpressed during culture in microgravity, deleting the over- or underexpressed RNA in bacteria, and killing or attenuating the bacteria to produce a vaccine. In other examples, the method comprises culturing bacteria in microgravity, evaluating RNA expression, detecting a RNA that is over- or underexpressed during culture in microgravity, selecting bacteria that over- or underexpress the RNA, culturing the selected bacteria, and killing the bacteria to produce a vaccine. Vaccine compositions produced by the disclosed methods are also contemplated.Type: ApplicationFiled: March 25, 2009Publication date: October 15, 2009Inventors: Timothy G. Hammond, Patricia L. Allen
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Publication number: 20090252828Abstract: Disclosed are compositions comprising variants of alpha-amylase that have alpha-amylase activity and which exhibit altered properties relative to a parent AmyS-like alpha-amylase from which they are derived. The compositions comprise an additional enzyme such as a phytase. Also disclosed are methods of using the compositions, and kits related thereto.Type: ApplicationFiled: November 3, 2008Publication date: October 8, 2009Applicant: Danisco US Inc., Genencor DivisionInventors: Luis G. Cascao-Pereira, James T. Kellis, JR., Bradley A. Paulson, Scott D. Power, Sandra W. Ramer, Vivek Sharma, Andrew Shaw, Jayarama K. Shetty, Donald E. Ward
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Publication number: 20090239267Abstract: The present invention provides a method for producing an L-amino acid using a bacterium of the Enterobacteriaceae family, particularly a bacterium belonging to genus Escherichia or Pantoea, which has been modified to attenuate expression of the cpxR gene.Type: ApplicationFiled: January 22, 2008Publication date: September 24, 2009Inventors: Konstantin Vyacheslavovich Rybak, Aleksandra Yurievna Skorokhodova, Elvira Borisovna Voroshilova, Tatyana Viktorovna Leonova