Culture Media, Per Se Patents (Class 435/253.6)
  • Patent number: 10722543
    Abstract: Methods of inhibiting or reducing tumor metabolism and growth are disclosed. A composition containing oxygen scavenging membrane fragments and anaerobe bacteria is injected into a tumor to interfere with tumor growth and metabolism, leading to tumor necrosis. The composition may also contain a cryoprotectant, which permits the composition to be stored at sub-zero temperatures without freezing.
    Type: Grant
    Filed: January 26, 2017
    Date of Patent: July 28, 2020
    Assignee: Oxyrase, Inc.
    Inventor: James C. Copeland
  • Patent number: 10625232
    Abstract: The invention relates to a method for treating nanofibrillar cellulose hydrogel, wherein the method comprises the steps of: providing a nanofibrillar cellulose hydrogel; and subjecting the nanofibrillar cellulose hydrogel to heat treatment, wherein the heat treatment comprises keeping the nanofibrillar cellulose hydrogel at a temperature of at least 130° C. for at least 0.5 seconds, for reducing the number of viable micro-organisms in the nanofibrillar cellulose hydrogel.
    Type: Grant
    Filed: December 21, 2015
    Date of Patent: April 21, 2020
    Assignee: UPM-KYMMENE CORPORATION
    Inventors: Antti Laukkanen, Esa Laurinsilta
  • Patent number: 10563167
    Abstract: Provided is a microorganism culture sheet having an excellent efficiency for collection of environmental microorganisms and a method for measuring the environmental microorganisms with an excellent collection efficiency. Provided is a microorganism culture sheet including a base sheet, a dry culture layer formed on the base sheet, and a cover sheet covering the dry culture layer, wherein an adhesive layer and a release film are laminated on the inner surface of the cover sheet. Because the environmental microorganisms are collected using the adhesive layer, an excellent collection efficiency is provided.
    Type: Grant
    Filed: January 26, 2011
    Date of Patent: February 18, 2020
    Assignee: Dai Nippon Printing Co., Ltd.
    Inventors: Shin-ichi Waku, Michiko Miyagi, Rui Saito, Tetsuji Ueki, Takuma Baba, Mai Kinoshita
  • Patent number: 9597266
    Abstract: An object of the present invention is to provide a composition for external application that is applied to a part of the skin to more effectively elicit the useful effects of an adenine compound such as adenine, adenosine, and a phosphoric ester of adenosine, and that has long-lasting effects and enhanced skin-moisturizing effects. A gel composition for external application containing an adenine compound such as adenine, adenosine, and a phosphoric ester of adenosine is prepared by using agar as a gelling agent. The gel composition is further prepared in the form of a sheet-like adhesive patch. The gel composition in the form of a sheet-like adhesive patch is kept immersed in an aqueous solution containing the adenine compound.
    Type: Grant
    Filed: August 4, 2008
    Date of Patent: March 21, 2017
    Assignee: OTSUKA PHARMACEUTICAL CO., LTD.
    Inventors: Misato Nakajo, Fumiki Harano, Kosaburo Wakamatsu, Kazuhiro Yagyu
  • Patent number: 9029118
    Abstract: Selective enrichment media and methods for selectively growing and detecting Salmonella spp. The media comprise a carbon and nitrogen source, an inorganic salt, a fermentable sugar, one or more selective agents, and an efflux pump inhibitor. Various selective agents include sulfa drugs, surfactants, aminocoumarins, cycloheximide, supravital stains, ascorbic acid, bromobenzoic acid, myricetin, rifamycins, polyketides, and oxazolidinones. Various efflux pump inhibitors include arylpiperazines, such as 1-(1-naphthylmethyl)piperazine, and quinoline derivatives, such as 4-chloroquinoline. The selective agents and efflux pump inhibitors are provided in the media in combinations and amounts that inhibit growth of non-Salmonella microorganisms without substantially affecting growth and metabolism of Salmonella species. Methods of selectively growing and detecting Salmonella species are provided.
    Type: Grant
    Filed: February 27, 2013
    Date of Patent: May 12, 2015
    Assignee: Paradigm Diagnostics, Inc.
    Inventor: Alan D Olstein
  • Publication number: 20150125935
    Abstract: A hyperbranched polymer based on one or more repeating units of an ABx type monomer, wherein A and B are functional groups and x is greater than or equal to 2, wherein A reacts with, or substantially reacts with, B, wherein B is fractionally functionlized with a plurality of functional groups comprising a first functional group comprising a C6-C30 alkyl chain attached to the repeating unit through a carbonyl group (C?O) via an ester linkage, a second functional group comprising a partially fluorinated or perfluorinated C3-C20 alkyl chain attached to the repeating unit through a carbonyl group (C?O) via an ester linkage, and a third functional group comprising substantially one of a stabilized radical source attached to the repeating unit via a C0-C6 tether, or a 5 to 8 member chloroamide heterocycle of carbon and nitrogen that is attached to the repeating unit via a C2-C6 tether.
    Type: Application
    Filed: January 5, 2015
    Publication date: May 7, 2015
    Inventors: Andre A. Williams, Joshua A. Orlicki
  • Publication number: 20150111283
    Abstract: There are disclosed a culture media and culture supplements for cultivating microorganisms, including Listeria spp and methods for culturing microorganisms including Listeria spp. There are also disclosed methods for detecting the presence of microorganisms including Listeria spp in samples.
    Type: Application
    Filed: November 10, 2014
    Publication date: April 23, 2015
    Inventors: Gabriela Martinez, David Claveau, Lila Maduro
  • Patent number: 8951756
    Abstract: A method for cultivating a bacterial cell comprising the addition of an amino acid in an alkaline solution used for pH regulation. Also an aspect is a method for producing a polypeptide comprising the steps of a) providing a bacterial cell comprising a nucleic acid encoding the polypeptide, b) cultivating the provided cell, c) adjusting the pH value during the cultivating with a basic solution comprising an amino acid, d) recovering the polypeptide from the cell or the cultivation medium and thereby producing the polypeptide.
    Type: Grant
    Filed: August 25, 2011
    Date of Patent: February 10, 2015
    Assignee: Hoffmann-La Roche Inc.
    Inventor: Christian Schantz
  • Publication number: 20150024449
    Abstract: A process for fermenting syngas and a fermentation medium provides high ethanol productivity while removing medium components that were previously thought to be essential. The process is effective for providing a specific STY of at least about 1 gram of ethanol/(L·day·gram cells) and for providing a selenium content in cell biomass exiting the fermentation of about 1 ppm or less.
    Type: Application
    Filed: July 15, 2014
    Publication date: January 22, 2015
    Applicant: INEOS BIO SA
    Inventors: Frédéric Kendirgi, Ryan H. Senaratne, Syrona R. Scott
  • Patent number: 8932851
    Abstract: The present invention relates to an improved culture medium and method for the enhanced growth and detection of Mycobacterium growth. The invention further relates to an improved mycobacterial reagent system or kit that can be used for the enhanced growth and detection of Mycobacterium.
    Type: Grant
    Filed: January 15, 2013
    Date of Patent: January 13, 2015
    Assignee: bioMērieux, Inc.
    Inventors: Parampal Deol, Leticia Barton, Yoany Portilla, Douglas Lovern
  • Publication number: 20150004673
    Abstract: The present invention relates to natural crude salt formulations arid their cost-effective preparation. In particular, the invention discloses the fractionation of seawater into three fractions which can thereafter be suitably combined to reconstitute seawater in its pristine form for its various applications such as growth of marine microbes, flora and fauna in a marine environment, especially where such environments have to be created in locations far removed from the sea and/or where there is a requirement to modify the composition of seawater to better its performance. The fractions can also be blended appropriately to exclude the major constituent, namely sodium chloride, and thereby be useful for mineralization/re-mineralization of waters which are deficient in mineral nutrients such as calcium, magnesium, potassium, sulphate and bicarbonate including rain water and desalinated waters obtained through thermal/RO desalination.
    Type: Application
    Filed: December 27, 2012
    Publication date: January 1, 2015
    Inventors: Pushpito Kumar Ghosh, Sumesh Chandra Upadhyay, Sandhya Chandrikaprasad Mishra, Vadakke Puthoor Mohandas, Divesh Narayan Srivastava, Vinod Kumar Shahi, Rahul Jasvantrai Sanghavi, Sreekumaran Thampy, Babulal Surabhai Makwana, Imran Pancha, Ruma Pal, Ramkrishna Sen
  • Publication number: 20150005248
    Abstract: The present invention relates to methods for culturing spirochetes, in particular Borrelia burgdorferi. The present invention also provides methods of identifying spirochetes present in a biological sample. The present invention further provides methods of diagnosing diseases cause by a spirochete infection, such as Lyme disease, syphilis, and multiple sclerosis. The present invention further provides methods for identifying spirochete susceptibilities to antimicrobials and antimicrobial compositions and cocktails. The present invention also provides methods for treating subjects suspected of having a spirochete infection.
    Type: Application
    Filed: July 23, 2014
    Publication date: January 1, 2015
    Inventors: Eva T. Sapi, Timothy R. Schwartz, Namrata Pabbati
  • Patent number: 8921068
    Abstract: A method and point of use kit to determine under field conditions the presence and/or amount of a selected Pseudomonas species capable of bioremediating organic environmental pollutants, such as petroleum hydrocarbons.
    Type: Grant
    Filed: April 24, 2013
    Date of Patent: December 30, 2014
    Assignee: CL Solutions, LLC
    Inventors: Michael T. Saul, Sergey Gazenko
  • Publication number: 20140349377
    Abstract: A composition for fermentation or microbial culture of gram-positive bacteria, includes fibril cellulose and at least one nutrient source including at least one carbon source, at least one nitrogen source, at least one phosphorus source, at least one mineral source and at least one trace element source.
    Type: Application
    Filed: December 18, 2012
    Publication date: November 27, 2014
    Inventors: Marko Lauraeus, Antti Laukkanen
  • Publication number: 20140342438
    Abstract: A media supplement for culturing anaerobic bacteria is provided which comprises a filtrate of effluent from a chemostat vessel in which a target bacterial ecosystem has been cultured. Methods of using the supplement for culturing or isolating anaerobic microbial strains or communities, particularly anaerobic bacteria from the human gut, are also provided.
    Type: Application
    Filed: September 14, 2012
    Publication date: November 20, 2014
    Applicant: UNIVERSITY OF GUELPH
    Inventors: Emma Allen-Vercoe, Julie McDonald
  • Patent number: 8883442
    Abstract: There are disclosed a culture media and culture supplements for cultivating microorganisms, including Listeria spp and methods for culturing microorganisms including Listeria spp. There are also disclosed methods for detecting the presence of microorganisms including Listeria spp in samples.
    Type: Grant
    Filed: July 11, 2012
    Date of Patent: November 11, 2014
    Assignee: Foodchek Systems, Inc.
    Inventors: Gabriela Martinez, David Claveau, Lila Maduro
  • Publication number: 20140322807
    Abstract: Processes for extracting and recovering nutrients from organic wastes to create a cell culture broth for microorganisms involve the main steps of mixing, solid/liquid separation, optimization, and sterilization. In an embodiment, the method for converting organic waste material into a cell culture broth or growth media includes: (a) mixing an organic waste material with one or more solvent to create a mixture of liquids and solids under substantially turbulent conditions; (b) separating the mixture of liquids and solids into a liquid stream and solid stream; and (c) sterilizing the liquid stream, whereby the cell culture broth or growth media comprises the sterilized liquid stream.
    Type: Application
    Filed: December 6, 2012
    Publication date: October 30, 2014
    Applicant: INNOVATIVE BIOS L.L.C.
    Inventors: John Paul Gustin, Jason Allen Repac
  • Patent number: 8871465
    Abstract: The present invention relates to a method for identifying bacteria of the Bacillus cereus group, comprising the following steps: (a) providing a sample that may contain bacteria of the Bacillus cereus group, a reaction medium comprising at least one fluorescent phosphatidylcholine phospholipase C (PC-PLC) substrate and an inhibitor of Gram-negative bacteria; (b) inoculating the reaction medium with the sample; (c) incubating the inoculated reaction medium; and (d) identifying the bacteria of the Bacillus cereus group by detecting the PC-PLC substrate hydrolysis reaction, in which the pH of the reaction medium and the time necessary for detecting the PC-PLC substrate hydrolysis reaction are adapted such that said hydrolysis reaction by bacteria of the Bacillus cereus group is detected before hydrolysis of the PC-PLC substrate by any Gram-positive bacteria other than those belonging to the Bacillus cereus group, that may be present in the sample.
    Type: Grant
    Filed: September 16, 2010
    Date of Patent: October 28, 2014
    Assignee: bioMérieux, S.A.
    Inventors: Marie Cellier, John Mills, David Mosticone, Sylvain Orenga, Antoine Vimont
  • Patent number: 8859235
    Abstract: A method for the manufacture of products by biotechnological methods in bacterial cell culture is disclosed as well as products obtained, the use of certain additives to the media used in the manufacture of said products in bacterial cell culture media, and the use of said additives in reducing the detrimental effects of radicals in the manufacture of the products, as well as aspects related to these invention embodiments. The manufacturing process or method comprises adding one or more radical scavenging and/or antioxidative additive preferably selected from the group consisting of sterically hindered nitroxyls, sterically hindered hydroxylamines, sterically hindered hydroxylamine salt compounds, sterically hindered amino compounds and sterically hindered N-hydrocarbyloxyamines, benzofuranone compounds, as obligatory component(s) to the medium used during biosynthesis.
    Type: Grant
    Filed: August 11, 2010
    Date of Patent: October 14, 2014
    Assignee: BASF SE
    Inventors: Franz Kaufmann, Trine Hefsgaard Miller, Johannes Flothmann, Beate Winter, Andreas Hafner
  • Publication number: 20140295482
    Abstract: Methods and compositions for enhancing or promoting germination of bacterial spores, and yeasts are disclosed herein. The composition of the present invention comprises an extract obtained from banana or any member belonging to the genus Musa that may be used alone or in a growth medium to promote and enhance germination of bacterial spores, growth of bacterial, yeast, and fungal cell cultures.
    Type: Application
    Filed: November 2, 2012
    Publication date: October 2, 2014
    Inventor: Mark Lyte
  • Patent number: 8846334
    Abstract: The disclosure provides culture devices and methods useful for detecting acid-producing bacteria in a sample. The devices include a nutrient medium and a pH indicator to detect and differentiate acid-producing microorganisms, such as lactic acid bacteria. Methods of use include detecting or enumerating acid-producing microorganisms. The methods further provide for the detection of gas-producing acid-producing bacteria.
    Type: Grant
    Filed: June 15, 2010
    Date of Patent: September 30, 2014
    Assignee: 3M Innovative Properties Company
    Inventors: Robert F. Young, Patrick A. Mach, Michael E. Hughes, Christine A. Binsfeld, Jason W. Bjork, Mara S. Reif-Wenner, Henry J. Lubrant
  • Patent number: 8841112
    Abstract: The present invention relates to methods for culturing spirochetes, in particular Borrelia burgdorferi. The present invention also provides methods of identifying spirochetes present in a biological sample. The present invention further provides methods of diagnosing diseases cause by a spirochete infection, such as Lyme disease, syphilis, and multiple sclerosis. The present invention further provides methods for identifying spirochete susceptibilities to antimicrobials and antimicrobial compositions and cocktails. The present invention also provides methods for treating subjects suspected of having a spirochete infection.
    Type: Grant
    Filed: November 22, 2013
    Date of Patent: September 23, 2014
    Assignee: Advanced Laboratory Services, Inc.
    Inventors: Eva T. Sapi, Timothy Robert Schwartz, Namrata Pabbati
  • Publication number: 20140273143
    Abstract: Methods, systems, and compositions for growing high density cultures of dechlorinating microorganisms, such as the bacteria Dehalococcoides. Dechlorinating cultures are grown in continuous flow stirred-tank reactors at short hydraulic retention time, resulting in improved batch production. For some cultures, a culture medium including chlorine containing compounds, bicarbonate, and HEPES utilized.
    Type: Application
    Filed: March 11, 2014
    Publication date: September 18, 2014
    Applicant: Arizona Board of Regents on behalf of Arizona State University
    Inventors: Rosa Krajmalnik-Brown, Cesar Torres, Anca Delgado, Sudeep Popat, Devyn Fajardo-Williams
  • Patent number: 8828653
    Abstract: The present invention refers to articles for collecting samples from a surface, articles for microbiological analyses of said samples, and methods of use of said articles. The articles include sample collectors, sample housings with optional barrier layers, and sample-ready reagent strips comprising hydrophilic agents to grow and detect microorganisms. The disclosure includes methods to collect, detect, and quantify microorganisms in a surface sample.
    Type: Grant
    Filed: November 20, 2008
    Date of Patent: September 9, 2014
    Assignee: 3M Innovative Properties Company
    Inventors: Cynthia D. Zook, Robert H. Silbernagel, Barbara L. Horter, Henry J. Lubrant, Robert E. Koeritzer
  • Patent number: 8828725
    Abstract: A culture medium is used for culturing neural cells. Each neural cell includes a neural cell body and at least one neurite branched from the neural cell body. The culture medium includes a substrate and a carbon nanotube structure located on the substrate. The carbon nanotube structure includes a number of carbon nanotube wires spaced apart from each other. A distance between adjacent carbon nanotube wires is greater than or equal to diameters of the neural cell bodies. The carbon nanotube wires are capable of guiding extending directions of the neurites.
    Type: Grant
    Filed: August 1, 2012
    Date of Patent: September 9, 2014
    Assignees: Tsinghua University, Hon Hai Precision Industry Co., Ltd.
    Inventors: Li Fan, Chen Feng, Wen-Mei Zhao
  • Publication number: 20140242673
    Abstract: This invention belongs to the technical field of industrial microorganism fermentation and relates to a type of chemical defined medium used for succinic acid production by fermentation and its application. The chemical defined medium of this invention used for succinic acid production by fermentation includes conventional compositions and critical growth factor compositions. Said critical growth factor compositions include biotin or 5-ALA, niacin, amino acid, and methionine. This formula determines four critical growth factors of succinic acid fermentation through reasonable technical means. The culture medium is free of complicated and expensive nutritious compositions such as yeast powder and peptone.
    Type: Application
    Filed: September 14, 2012
    Publication date: August 28, 2014
    Inventors: Min Jiang, Yongian Xi, Jiuhua Zhang, Rong Xu, Wenyu Dai, Kequan Chen, Ping Wei
  • Publication number: 20140234889
    Abstract: The present invention is directed to a method and means for the neutralization, binding, and/or inactivation of antimicrobials in a test sample. The invention is also directed to a method of detecting the presence of one or more microorganisms in a test sample by culturing the test sample in a culture media comprising one or more primary amine-containing compounds.
    Type: Application
    Filed: November 1, 2013
    Publication date: August 21, 2014
    Applicant: bioMerieux, Inc.
    Inventor: Douglas Lovern
  • Patent number: 8802408
    Abstract: The present invention relates to a process for preparing a nutritional, therapeutic or organoleptic product by growing non-recombinant yeast under aerobic conditions, in a medium that includes crude glycerol, as one possible carbon source to produce a yeast product. The yeast product can be processed to obtain such nutritional, therapeutic or organoleptic products as yeast paste, yeast metabolites, carbohydrates, proteins, functional proteins, nucleotides, yeast autolysates, yeast extract, yeast cell walls, beta-glucans, mannans or a product derived from a mineralized yeast product.
    Type: Grant
    Filed: January 16, 2009
    Date of Patent: August 12, 2014
    Assignee: Bio Processing Australia Pty Ltd.
    Inventors: Robin Fieldhouse, Donald Finlay MacLennan, David Graham MacLennan, Mary Elizabeth MacLennan
  • Patent number: 8790912
    Abstract: The composition comprises, by mass, about: 52% Lactose, 15.2% B12-fortified Brewer's Yeast, 12% Sodium Proponate, 0.8% Sodium carbonate, 0.12% Nitrate Nitrogen, 2.1% Ammoniacal Nitrogen, 3.78% Urea Nitrogen, 1% Available Phosphoric Acid, 0.4% Soluble Potash (K2O), 2% Sulphur (S), 0.0064% Iron, 0.0066% Manganese, 0.0036% Zinc, 0.00068% Copper, 0.003% Boron and about 0.0068% Molybdenum.
    Type: Grant
    Filed: May 28, 2010
    Date of Patent: July 29, 2014
    Inventors: Ivan Richard Schaffner, Jr., Kent C. Armstrong
  • Patent number: 8778657
    Abstract: The present invention discloses culture medium unit doses for cultivating microorganisms comprising at least two compositions, each composition packaged in a composition unit dose of a predetermined amount, the composition unit doses being used for combining one of each composition unit dose forming the culture medium unit dose. The composition unit doses being packaged separately and individually until a time the culture medium unit dose is to be prepared for use for cultivation of microorganisms, wherein the time one of each composition unit dose are combined thereby forming the culture medium unit dose. The invention also discloses a method of manufacturing the composition unit doses, and a kit for cultivating microorganisms, the kit comprising a combination of the composition unit doses.
    Type: Grant
    Filed: October 17, 2012
    Date of Patent: July 15, 2014
    Assignee: Hardy Diagnostics
    Inventors: Gary A. Peterson, Andre Y. Hsiung
  • Publication number: 20140187818
    Abstract: The invention relates to a bacterial strain belonging to the genus Rhodococcus which is a producer of a nitrile hydratase. The invention also relates to a method for producing acrylamide by hydration of acrylonitrile using a biomass of the bacterial strain and to a method of culturing the bacterial strain.
    Type: Application
    Filed: December 24, 2013
    Publication date: July 3, 2014
    Inventors: Sergey Vladimirovich Kozulin, Tatiana Nicolaevna Kozulina, Alexey Sergeevich Kozulin
  • Patent number: 8765411
    Abstract: The subject invention provides an improved process for the production of human growth hormone.
    Type: Grant
    Filed: September 20, 2011
    Date of Patent: July 1, 2014
    Assignee: Ferring B.V.
    Inventors: Dov Kanner, Eli Schmell
  • Publication number: 20140162345
    Abstract: Processes for converting lignocellulose to feedstock and downstream products are disclosed. The processes may include acid treatment of lignocellulose to produce a fermentation feedstock. In various instances, the processes include recovery or recycling of acid, such as recovery of hydrochloric acid from concentrated and/or dilute streams. Downstream products may include acrylic acid-based products such as diapers, paper and paper-based products, ethanol, biofuels such as biodiesel and fuel additives, and detergents.
    Type: Application
    Filed: April 2, 2012
    Publication date: June 12, 2014
    Inventor: Aharon Eyal
  • Publication number: 20140162363
    Abstract: The invention generally provides a cell culture vessel having at least one first zone and at least one second zone, wherein the first zone is a transfer zone for a culture medium which essentially contains no cells and the second zone is a cell culture zone. The invention further includes methods utilizing the cell culture vessel.
    Type: Application
    Filed: December 2, 2013
    Publication date: June 12, 2014
    Applicant: ARTELIS S.A.
    Inventor: Jose Antonio CASTILLO FERNANDEZ
  • Publication number: 20140147467
    Abstract: The present invention relates to new contamination resistant artificial media for the routine cultivation of P. salmonis. In particular, it discloses first a liquid media based named Austral-SRS Broth that extensively require iron and sodium chloride; and, second, a blood-free agar media comprising tryptone soy with either ferric or ferrous salts (Austral-TSFe) agar or the use of hemoglobin (Austral-TSHem) as a source of iron in the agar. Also disclosed is a method for their preparation as well as their application in the development of antigens and low cost vaccines for protection against Piscirickettsiosis and the use of the medias for a kit that evaluate the antibiotic resistance appearance in different strain of P. salmonis isolated from the salmon industry.
    Type: Application
    Filed: November 26, 2012
    Publication date: May 29, 2014
    Inventors: Alejandro Javier Yanez Carcamo, Juan Guillermo Carcamo, Victor Hugo Olavarria
  • Publication number: 20140148621
    Abstract: At least one isolated microorganism and a fermentation method to convert hydrogen gas, carbon dioxide gas, and/or carbon monoxide gas to a lower alkyl alcohol and/or carboxylic acid and to produce at least 2% by volume of the lower alkyl alcohol or carboxylic acid in an aqueous-based medium.
    Type: Application
    Filed: August 7, 2013
    Publication date: May 29, 2014
    Inventors: Richard A. KOHN, Seon-Woo Kim
  • Publication number: 20140141493
    Abstract: Disclosed are recombinant host cells suitable for degrading an oligosaccharide that have been optimized for growth and production of high yields of ethanol, and methods of making and using these cells. The invention further provides minimal media comprising urea-like compounds for economical production of ethanol by recombinant microorganisms. Recombinant host cells in accordance with the invention are modified by gene mutation to eliminate genes responsible for the production of unwanted products other than ethanol, thereby increasing the yield of ethanol produced from the oligosaccharides, relative to unmutated parent strains. The new and improved strains of recombinant bacteria are capable of superior ethanol productivity and yield when grown under conditions suitable for fermentation in minimal growth media containing inexpensive reagents. Systems optimized for ethanol production combine a selected optimized minimal medium with a recombinant host cell optimized for use in the selected medium.
    Type: Application
    Filed: January 6, 2014
    Publication date: May 22, 2014
    Applicant: UNIVERSITY OF FLORIDA RESEARCH FOUNDATION, INC.
    Inventors: Lonnie O' Neal Ingram, Lorraine P. Yomano, Sean W. York, Brent E. Wood
  • Patent number: 8728796
    Abstract: Methods, processes and materials for the production and recovery of Tiacumicins produced by culturing a microorganism belonging to the species Dactylosporangium aurantiacum subspecies hamdenensis having the ability to produce and accumulate one or more Tiacumicin in a nutrient medium comprising a carbon source, a nitrogen source, trace elements such as inorganic salts, and an adsorbent, wherein said nitrogen source comprises fish powder, and wherein said Tiacumicin is produced in a yield greater than about 50 mg/L broth.
    Type: Grant
    Filed: June 12, 2012
    Date of Patent: May 20, 2014
    Assignee: Optimer Pharmaceuticals, Inc.
    Inventors: Youe-Kong Shue, Chi-Jen Frank Du, Ming-His Chiou, Mei-Chiao Wu, Yuan-Ting Chen, Franklin W. Okumu, Jonathan James Duffield
  • Publication number: 20140134705
    Abstract: The present invention is directed to environmental remediation, and specifically to materials, systems, and methods for oil spill clean up. A system for oil spill containment incorporates an oil adsorbing material and bacterial nutrient material. The oil adsorbing material may be a polymeric material. The polymeric material may be one or more of a synthetic polymer and a natural product. Suitable synthetic polymers include polypropylene, polyethylene, polyurethane, polyester, and combinations thereof. The synthetic polymer may be recycled. Suitable natural produces include palm oil and beeswax. The bacterial nutrient may be organic or inorganic. A suitable organic bacterial nutrient is bee pollen. Suitable inorganic bacterial nutrients include nitrates and phosphates. A method for containing oil in an oil spill at a location incorporates applying the oil spill containment system to the oil and removing the oil and the oil spill containment system from the location.
    Type: Application
    Filed: June 25, 2012
    Publication date: May 15, 2014
    Applicant: ROK INVESTMENT GROUP LIMITED
    Inventor: James Jackson Milham Henry
  • Patent number: 8709746
    Abstract: A reaction medium for the culture and/or detection and/or identification of bacteria of the Legionella genus includes at least one siliceous compound, where the siliceous compound is a nonpolar silica. A method for detecting and/or identifying bacteria of the Legionella genus, includes bringing a sample that may contain bacteria of the Legionella genus into contact with a reaction medium comprising at least one siliceous compound, incubating, and detecting the presence of bacteria of the Legionella genus.
    Type: Grant
    Filed: October 14, 2009
    Date of Patent: April 29, 2014
    Assignee: bioMerieux
    Inventors: Marie Cellier, Sylvain Orenga, Denis Robichon, Veronique Sauvonnet
  • Patent number: 8709516
    Abstract: A method for culturing lactic acid bacteria includes (a) adding a proteolytic enzyme to an aqueous whey solution containing whey and water to thereby prepare a culture solution containing whey degraded by the proteolytic enzyme; (b) inoculating the culture solution with Lactobacillus gasseri which is a bacteriocin-producing lactic acid bacteria; and (c) maintaining the culture solution inoculated with the bacteriocin-producing lactic acid bacteria at a pH of not lower than 5.2 and not higher than 5.8 to provide cultured lactic acid bacteria. After the completion of culturing, the culture solution is centrifuged to separate a concentrated cell suspension containing the lactic acid bacterium in a concentrated form. The concentrated cell suspension has extremely high antibacterial activity, several tens of thousands AU, and is usable as a food preservative. Yogurt is produced by fermenting a yogurt mix to which 0.01% to 0.1% by weight of the concentrated cell suspension is added.
    Type: Grant
    Filed: March 26, 2010
    Date of Patent: April 29, 2014
    Assignee: Meiji Co., Ltd.
    Inventors: Kakuhei Isawa, Masayuki Kamijo
  • Publication number: 20140106436
    Abstract: The invention relates to the use of at least one chelating agent, including an iron chelating agent, in order to stimulate the growth of magnetotactic bacteria.
    Type: Application
    Filed: May 4, 2012
    Publication date: April 17, 2014
    Applicants: CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE, UNIVERSITE PIERRE ET MARIE CURIE (PARIS 6)
    Inventors: Edouard Alphandery, Imène Chebbi
  • Patent number: 8697419
    Abstract: The present invention relates to methods for culturing spirochetes, in particular Borrelia burgdorferi. The present invention also provides methods of identifying spirochetes present in a biological sample. The present invention further provides methods of diagnosing diseases cause by a spirochete infection, such as Lyme disease, syphilis, and multiple sclerosis. The present invention further provides methods for identifying spirochete susceptibilities to antimicrobials and antimicrobial compositions and cocktails. The present invention also provides methods for treating subjects suspected of having a spirochete infection.
    Type: Grant
    Filed: September 25, 2012
    Date of Patent: April 15, 2014
    Assignee: Advanced Laboratory Services, Inc.
    Inventors: Eva T. Sapi, Timothy Robert Schwartz, Namrata Pabbati
  • Patent number: 8679820
    Abstract: In one embodiment, a bioremediation agent and method of bioremediation of an oil spill are disclosed. The bioremediation agent contains oil-digesting bacteria and bacterial nutrients in a buoyant water semi-insoluble and biodegradable casein product. The bioremediation agent may be distributed by boats or seeded by aircraft to remediate oil from coastlines after an oil spill.
    Type: Grant
    Filed: October 15, 2011
    Date of Patent: March 25, 2014
    Inventor: John P. Fuhrer
  • Patent number: 8673617
    Abstract: The invention relates to a culture medium for Haemophilus influenzae type b, characterized in that the source of protein nitrogen is of non-animal origin and comprises at least one plant peptone and in that the heme source consists of protoporphyrin IX. This medium serves in particular for the production of polyribosyl phosphate (PRP) and for the manufacture of a vaccine against Haemophilus influenzae type b meningitis.
    Type: Grant
    Filed: July 10, 2008
    Date of Patent: March 18, 2014
    Assignee: Sanofi Pasteur SA
    Inventors: Ghislaine Maitre-Wilmotte, Denis Speck, Bachra Rokbi
  • Publication number: 20140038229
    Abstract: Provided are methods for generating a microorganism with improved ability to convert glycerol to succinic acid. These methods comprise combining a microorganism with ability to convert glycerol to succinic acid and a medium containing glycerol to produce a fermentation mixture; allowing fermentation to occur such that succinic acid is produced; and assaying for an indication of glycerol metabolism. These latter two steps may be repeated as necessary. Also provided are microorganisms generated in this manner, particularly bacteria deposited at the NCIMB on 13th April 2011 with the Accession Number NCIMB 41825. A method of producing succinic acid from glycerol is also provided, this method comprising: mixing a microorganism according to claim of the invention with a medium comprising glycerol to produce a fermentation mixture; and incubating the fermentation mixture, under conditions that promote fermentation to produce succinic acid, until succinic acid is produced.
    Type: Application
    Filed: April 20, 2012
    Publication date: February 6, 2014
    Applicant: THE UNIVERSITY OF MANCHESTER
    Inventor: Constantinos Theodoropoulos
  • Publication number: 20140024075
    Abstract: Provided herein are methods and compositions for bacterial cellulose production. In some embodiments, the methods and compositions involve or are made from distiller's grain.
    Type: Application
    Filed: May 15, 2012
    Publication date: January 23, 2014
    Applicant: Shanghai Zhiyi Information Technology Ltd.
    Inventor: Feng Hong
  • Publication number: 20140004090
    Abstract: A method of enhancing the antimicrobial properties of a bacterial strain, comprising cultivation of the bacterial strain in a medium comprising water, at least one component chosen from bee pollen, pollen, and bee bread, and optionally honey and/or nectar is described. The bacterial strain has the ability to be viable for at least 8 days in a 65% by weight sugar solution, preferably at least 8 days in a 70% by weight sugar solution.
    Type: Application
    Filed: January 24, 2012
    Publication date: January 2, 2014
    Applicant: CONCELLAE AB
    Inventors: Alejandra Vasquez, Tobias Olofsson
  • Patent number: 8617872
    Abstract: The present invention relates to methods and compositions for culturing spirochetes and treating spirochetal diseases. For example, the present invention provides serum-free media for culturing spirochete bacteria in vitro. The present invention further provides methods for identifying spirochete susceptibilities to antimicrobials and antimicrobial compositions and cocktails. The present invention also provides methods for treating subjects suspected of having a spirochete infection.
    Type: Grant
    Filed: April 8, 2011
    Date of Patent: December 31, 2013
    Inventor: Stephen R. Wechter
  • Publication number: 20130344550
    Abstract: A method of producing bioplastics from algae, the method including processing algae to yield an aqueous phase containing glycerol, and fermenting the aqueous phase with a bioplastic-producing bacteria to yield bioplastics.
    Type: Application
    Filed: June 10, 2013
    Publication date: December 26, 2013
    Applicant: Utah State University
    Inventors: Charles Miller, Asif Rahman, Ronald Sims, Ashik Sathish, Renil Anthony