Yeast; Media Therefor Patents (Class 435/254.2)
Saccharomyces (Class 435/254.21)
Candida (Class 435/254.22)
Pichia (Class 435/254.23)
  • PENTOSE FERMENTING MICROORGANISMS
    Publication number: 20140377813
    Abstract: The invention provides a microbial eukaryotic cell capable of utilizing C5 sugars, in particular xylose. Another objective of the invention is to provide an improved protein sequence to enable eukaryotic cells to degrade C5 sugars. The present invention thus provides protein comprising an amino acid sequence having at least 75% identity, preferably 80% identity, most preferably 90% identity, most highly preferably 95% identity to SEQ ID NO. 2 or SEQ ID NO. 8 and having xylose-isomerase activity in a eukaryotic cell.
    Type: Application
    Filed: February 7, 2013
    Publication date: December 25, 2014
    Inventors: Zdravko Dragovic, Christian Gamauf, Christoph Reisinger, Ulrich Kettling
  • ALPHA-AMYLASE
    Publication number: 20140377407
    Abstract: This invention relates to a novel alpha-amylase, a process for its preparation and the use of the amylase. The invention relates to a newly identified polynucleotide sequence from Alicyclobacillus pohliae comprising a gene that encodes the novel alpha-amylase enzyme. The invention features the full length coding sequence of the novel gene as well as the amino acid sequence of the full-length functional protein of the gene. The invention also relates to methods of using these proteins in industrial processes, for example in food industry, such as the baking industry. Also included in the invention are cells transformed with a polynucleotide according to the invention suitable for producing these proteins and cells.
    Type: Application
    Filed: January 29, 2013
    Publication date: December 25, 2014
    Applicant: DSM IP ASSETS B.V.
    Inventor: Lucie Parenicova
  • ANTI-HER3 ANTIBODIES AND USES THEREOF
    Publication number: 20140377272
    Abstract: The present invention relates to antibodies binding to human HER3 (anti-HER3 antibody), methods for their production, pharmaceutical compositions containing said antibodies, and uses thereof.
    Type: Application
    Filed: September 8, 2014
    Publication date: December 25, 2014
    Applicant: ROCHE GLYCART AG
    Inventors: Birgit Bossenmaier, Nikolaos Dimoudis, Thomas Friess, Guy Georges, Irene Kolm, Hans-Willi Krell, Valeria Lifke, Ekkehard Moessner
  • ALCOHOL DEHYDROGENASES (ADH) USEFUL FOR FERMENTIVE PRODUCTION OF LOWER ALKYL ALCOHOLS
    Publication number: 20140377824
    Abstract: The invention relates to suitable candidate ADH enzymes for production of lower alkyl alcohols including isobutanol. The invention also relates to recombinant host cells that comprise such ADH enzymes and methods for producing lower alkyl alcohols in the same.
    Type: Application
    Filed: May 20, 2014
    Publication date: December 25, 2014
    Applicant: Butamax Advanced Biofuels LLC
    Inventors: Sriram Satagopan, Daniel P. O'keefe, Janardhan Gude
  • ANTI-HER3 ANTIBODIES AND USES THEREOF
    Publication number: 20140377273
    Abstract: The present invention relates to antibodies binding to human HER3 (anti-HER3 antibody), methods for their production, pharmaceutical compositions containing said antibodies, and uses thereof.
    Type: Application
    Filed: September 8, 2014
    Publication date: December 25, 2014
    Applicant: ROCHE GLYCART AG
    Inventors: Birgit Bossenmaier, Nikolaos Dimoudis, Thomas Friess, Guy Georges, Irene Kolm, Hans-Willi Krell, Valeria Lifke, Ekkehard Moessner
  • PROTEIN COMPLEX, BISPECIFIC ANTIBODY INCLUDING THE PROTEIN COMPLEX, AND METHOD OF PREPARATION THEREOF
    Publication number: 20140378664
    Abstract: A protein complex comprising a first polypeptide comprising a first antigen-binding region; a second polypeptide comprising a second antigen-binding region; and a linker connecting the first polypeptide and the second polypeptide, wherein the first antigen-binding region is a single stranded polypeptide comprising a first light chain antigen-binding region and a first heavy chain antigen-binding region, the second antigen-binding region is a single stranded polypeptide comprising a second light chain antigen-binding region and a second heavy chain antigen-binding region, and the linker connects the C-terminal of the first polypeptide and the N-terminal of the second polypeptide, and comprises a tag including a cleavable amino acid sequence at one terminal or both terminals of the linker; as well as a bispecific antibody derived from the protein complex, and related compositions and methods.
    Type: Application
    Filed: June 25, 2014
    Publication date: December 25, 2014
    Inventors: Hye young SUH, Jae II Lee, Su-Jeong Hwang
  • ANTI-KDR ANTIBODIES AND METHODS OF USE
    Publication number: 20140377282
    Abstract: The present invention provides anti-KDR monoclonal antibodies and related compositions, which may be used in any of a variety of therapeutic methods for the treatment of a variety of cancers, rheumatoid arthritis, diabetic retinopathy and other diseases associated with aberrant VEGF or KDR expression and/or activity.
    Type: Application
    Filed: November 1, 2012
    Publication date: December 25, 2014
    Applicant: APEXIGEN, INC.
    Inventors: Yaohuang Ke, Sum Wai Pierre Lee, Yongke Zhang, Guo-Liang Yu, Weimin Zhu
  • YEAST HAVING RESISTANCE TO FREEZING STRESS
    Publication number: 20140377408
    Abstract: The present invention provides yeast having increased resistance to freezing stress in which POG1 gene is inactivated, a method for producing such yeast, and a method for using such yeast in food production.
    Type: Application
    Filed: November 20, 2012
    Publication date: December 25, 2014
    Inventors: Hiroshi Takagi, Yu Sasano, Jun Shima, Yutaka Haitani
  • 4-1BB BINDING MOLECULES
    Publication number: 20140377255
    Abstract: The present disclosure provides isolated binding molecules that bind to human 4-1BB, nucleic acid molecules encoding an amino acid sequence of the binding molecules, vectors comprising the nucleic acid molecules, host cells containing the vectors, methods of making the binding molecules, pharmaceutical compositions containing the binding molecules, and methods of using the binding molecules or compositions.
    Type: Application
    Filed: May 13, 2014
    Publication date: December 25, 2014
    Applicant: PFIZER INC
    Inventors: Bianca Ahrens, Sangita M. Baxi, Timothy Scott Fisher, Richard Michael Jerome, Kathrin Ladetzki-Baehs, Theodore Oliphant, Leslie Lynne Sharp, Michael Tesar, Libbey Anne Yates, Moritz Zulley
  • Novel P. Pastoris Pastoris Promoters, and the Use Thereof to Direct Expression of Proteins in Yeast, Preferably Using a Haploid Mating Strategy
    Publication number: 20140377802
    Abstract: Novel promoters which are derived from P. pastoris pastoris which are inducible or repressible under specific growth conditions are provided. These promoters are useful for regulating the expression of a desired structural gene, e.g., a mammalian polypeptide. Particularly preferred is the use of these novel promoters to regulate gene expression in polyploidal yeast such as diploidal P. pastoris produced by mating or spheroplast fusion.
    Type: Application
    Filed: July 3, 2014
    Publication date: December 25, 2014
    Inventors: James M. CREGG, Ilya I. TOLSTORUKOV
  • Isoprene synthase variants for improved microbial production of isoprene
    Patent number: 8916370
    Abstract: The present invention provides methods and compositions comprising at least one isoprene synthase enzyme with improved catalytic activity and/or solubility. In particular, the present invention provides variant plant isoprene synthases for increased isoprene production in microbial host cells. Biosynthetically produced isoprene of the present invention finds use in the manufacture of rubber and elastomers.
    Type: Grant
    Filed: March 30, 2012
    Date of Patent: December 23, 2014
    Assignees: Danisco US Inc., The Goodyear Tire & Rubber Company
    Inventors: Richard R. Bott, Marguerite A. Cervin, James T. Kellis, Jr., Joseph C. McAuliffe, Andrei Miasnikov, Caroline M. Peres, Christopher Lee Rife, Derek H. Wells, Walter Weyler, Gregory M. Whited
  • MICROORGANISMS AND METHODS FOR PRODUCTION OF 4-HYDROXYBUTYRATE, 1,4-BUTANEDIOL AND RELATED COMPOUNDS
    Publication number: 20140371417
    Abstract: The invention provides non-naturally occurring microbial organisms having a 4-hydroxybutyrate, gamma-butyrolactone, 1,4-butanediol, 4-hydroxybutanal, 4-hydroxybutyryl-CoA and/or putrescine pathway and being capable of producing 4-hydroxybutyrate, wherein the microbial organism comprises one or more genetic modifications. The invention additionally provides methods of producing 4-hydroxybutyrate, gamma-butyrolactone, 1,4-butanediol, 4-hydroxybutanal, 4-hydroxybutyryl-CoA and/or putrescine or related products using the microbial organisms.
    Type: Application
    Filed: April 25, 2014
    Publication date: December 18, 2014
    Applicant: Genomatica, Inc.
    Inventors: Priti PHARKYA, Anthony P. BURGARD, Stephen J. VAN DIEN, Robin E. OSTERHOUT, Mark J. BURK, John D. TRAWICK, Michael P. KUCKINSKAS, Brian STEER
  • ENDOGLUCANASE 1B (EG1B) VARIANTS
    Publication number: 20140370552
    Abstract: The present invention provides endoglucanase 1b (EG1b) variants suitable for use in saccharification reactions. The present application further provides genetically modified fungal organisms that produce EG1b variants, as well as enzyme mixtures exhibiting enhanced hydrolysis of cellulosic material to fermentable sugars, enzyme mixtures produced by the genetically modified fungal organisms, and methods for producing fermentable sugars from cellulose using such enzyme mixtures.
    Type: Application
    Filed: December 18, 2012
    Publication date: December 18, 2014
    Inventors: Ee Lui Ang, Ellen D. Eberhard, Xiyun Zhang, Wei Zhang, Jing Tian, Derek J. Smith, Vesna Mitchell
  • METHOD AND CONSTRUCTS FOR DELIVERING DOUBLE STRANDED RNA tO PEST ORGANISMS
    Publication number: 20140373194
    Abstract: The present invention relates in generally to RNAi and its use in gene silencing. In particular, the present invention relates to methods and constructs for delivering double stranded RNA (dsRNA) to pest organisms.
    Type: Application
    Filed: May 22, 2014
    Publication date: December 18, 2014
    Applicant: DEVGEN N.V.
    Inventors: Thierry Andre Olivier Eddy Bogaert, Richard Zwaal, Geert Plaetinck, Jan Octaaf De Kerpel, Titus Jan Kaletta
  • MODIFIED MICROORGANISMS AND METHODS OF MAKING BUTADIENE USING SAME
    Publication number: 20140370564
    Abstract: The present disclosure generally relates to microorganisms that comprise one or more polynucleotides coding for enzymes in one or more pathways that catalyze a conversion of a fermentable carbon source to butadiene. Also provided are methods of using the microorganisms in industrial processes including, for use in the production of butadiene and products derived therefrom.
    Type: Application
    Filed: December 17, 2012
    Publication date: December 18, 2014
    Inventors: Mateus Schreiner Garcez Lopes, Avram Michael Slovic, Iuri Estrada Gouvea, Johana Rincones Perez, Lucas Pedersen Parizzi
  • Use of Synthetic Scaffolds for the Production of Biosynthetic Pathway Products
    Publication number: 20140370595
    Abstract: The present invention provides methods of producing a product or product precursor of a biosynthetic pathway in a genetically modified host cell. The present invention also provides genetically modified host cells comprising nucleic acids encoding a scaffold polypeptide and nucleic acids comprising nucleotide sequences encoding two or more enzymes in a biosynthetic pathway. The present invention further provides nucleic acids comprising nucleotide sequences encoding scaffold polypeptides, for use in a subject method.
    Type: Application
    Filed: June 25, 2014
    Publication date: December 18, 2014
    Inventors: John E. Dueber, Jay D. Keasling, Gabriel C. Wu, Ghulam Reza K. Malmirchegini
  • METHOD FOR PRODUCING ISOPROPANOL AND RECOMBINANT YEAST CAPABLE OF PRODUCING ISOPROPANOL
    Publication number: 20140370573
    Abstract: Isopropanol is produced with good productivity via fermentation processes. Specifically, isopropanol is produced at a high level in a medium by culturing recombinant yeast into which an acetoacetyl CoA synthase gene and a group of genes (isopropanol synthesis-related gene group) encoding a group of enzymes for synthesis of isopropanol from acetoacetyl CoA have been introduced.
    Type: Application
    Filed: July 29, 2014
    Publication date: December 18, 2014
    Applicant: TOYOTA JIDOSHA KABUSHIKI KAISHA
    Inventors: Masayoshi MURAMATSU, Satoshi YONEDA
  • METHODS FOR HYDROXYLATING PHENYLPROPANOIDS
    Publication number: 20140363858
    Abstract: Provided herein are methods for producing an ortho-hydroxylated phenylpropanoid. In one embodiment the method includes culturing a microbe that includes HpaBC activity in the presence of a phenylpropanoid substrate. Also provided are genetically engineered microbes engineered to have greater levels of HpaB and/or HpaC than a control microbe.
    Type: Application
    Filed: June 10, 2014
    Publication date: December 11, 2014
    Inventors: YAJUN YAN, YUHENG LIN
  • MICROORGANISMS AND METHODS FOR THE PRODUCTION OF 1,4-CYCLOHEXANEDIMETHANOL
    Publication number: 20140363864
    Abstract: A non-naturally occurring microbial organism includes a microbial organism having a 1,4-cyclohexanedimethanol pathway that includes at least one exogenous nucleic acid encoding a 1,4-cyclohexanedimethanol pathway enzyme expressed in a sufficient amount to produce 1,4-cyclohexanedimethanol. A method for producing 1,4-cyclohexanedimethanol includes culturing a non-naturally occurring microbial organism having a 1,4-cyclohexanedimethanol pathway. The pathway includes at least one exogenous nucleic acid encoding a 1,4-cyclohexanedimethanol pathway enzyme expressed in a sufficient amount to produce 1,4-cyclohexanedimethanol, under conditions and for a sufficient period of time to produce 1,4-cyclohexanedimethanol.
    Type: Application
    Filed: January 10, 2014
    Publication date: December 11, 2014
    Applicant: Genomatica, Inc.
    Inventors: Priti Pharkya, Mark J. Burk
  • COMPOSITIONS AND METHODS FOR THE DIAGNOSIS AND TREATMENT OF TUMOR
    Publication number: 20140363873
    Abstract: The present invention is directed to compositions of matter useful for the diagnosis and treatment of tumor in mammals and to methods of using those compositions of matter for the same.
    Type: Application
    Filed: May 8, 2014
    Publication date: December 11, 2014
    Applicant: Genentech, Inc.
    Inventors: Mark Dennis, Bonnee Rubinfeld, Paul Polakis
  • Methods of Generating Bioactive Peptide-bearing Antibodies and Compositions Comprising the Same
    Publication number: 20140363433
    Abstract: In one aspect, the invention provides a method of making a bioactive peptide-bearing antibody, or fragment thereof, comprising (a) engrafting the amino acid sequence of at least one bioactive peptide of interest into (i) at least one of CDR-H1, CDR-H2 or CDR-H3 of a heavy chain variable region comprising one or more chicken framework regions and/or (ii) at least one of CDR-L1, CDR-L2 or CDR-L3 of the light chain variable region comprising one or more chicken framework regions, and (b) determining whether the antibody has substantially the same biological activity as the bioactive peptide.
    Type: Application
    Filed: March 12, 2014
    Publication date: December 11, 2014
    Inventors: W. Jason Cummings, Patrick Gray, Larry Tjoelker, Munehisa Yabuki
  • Cyclic Peptide Production
    Publication number: 20140363844
    Abstract: An enzyme useful for producing cyclic peptides from linear peptide precursors and a gene encoding the enzyme are described. The enzyme is particularly useful for producing segetalins from linear presegetalin precursors. The linear presegetalin precursors may be derived from other linear presegetalin precursors farther upstream in the biosynthetic synthesis of the segetalin.
    Type: Application
    Filed: December 7, 2012
    Publication date: December 11, 2014
    Inventors: Carla Jann Siobhan Barber, Darwin Wilfred Reed, Janet Anne Condie, Sheila Diniwe Sherin Chiwocha, Patrick Smithers Covello
  • ANTI-HUMAN RESPIRATORY SYNCYTIAL VIRUS (RSV) ANTIBODIES AND METHODS OF USE
    Publication number: 20140363427
    Abstract: Provided herein are antibodies or antigen-binding fragments thereof that immunospecifically bind to the fusion (F) protein of Respiratory Syncytial Virus (RSV). Also provided are methods for of prevention, treatment and diagnosis of viral infection and/or the treatment of one more symptoms of RSV-mediated disease. Methods of generating antibodies that immunospecifically bind RSV F protein also are provided.
    Type: Application
    Filed: June 5, 2014
    Publication date: December 11, 2014
    Inventors: Robert Anthony Williamson, Jehangir Wadia, Gabriel Pascual, Elissa Keogh
  • NEUREGULIN ANTIBODIES AND USES THEREOF
    Publication number: 20140363438
    Abstract: The invention provides anti-neuregulin1 antibodies and methods of using the antibodies in treating diseases or disorders, such as cancer. In a particular embodiment, the anti-neuregulin1 antibodies bind to both neuregulin1? and neuregulin 1? isoforms.
    Type: Application
    Filed: August 16, 2012
    Publication date: December 11, 2014
    Applicant: Genentech, Inc.
    Inventors: Erica Jackson, Gabriele Schaefer, Yan Wu
  • Biofilm inhibiting composition
    Patent number: 8906393
    Abstract: Polysaccharide-containing extracts isolated from a host cell containing nucleotide sequences encoding genes pamA, pamB and pamC, wherein the extract is capable of inhibiting biofilm formation produced by gram-negative bacteria, gram-positive bacteria and fungi, and methods to inhibit biofilm formation or remove biofilms that have already formed.
    Type: Grant
    Filed: January 26, 2012
    Date of Patent: December 9, 2014
    Assignee: Rutgers, The State University of New Jersey
    Inventors: Jeffrey B. Kaplan, Nataliya V. Balashova, Scott C. Kachlany, Evguenii Vinogradov
  • esterase/lipase promoter regions for gene expression in yeast
    Patent number: 8906650
    Abstract: Promoter regions associated with the Yarrowia lipolytica esterase/lipase (EL1) gene are disclosed and have been found to be particularly effective for the expression of heterologous genes in yeast. These promoter regions will be useful for driving high-level expression of genes involved in the production of omega-3 and omega-6 fatty acids.
    Type: Grant
    Filed: March 30, 2012
    Date of Patent: December 9, 2014
    Assignee: E I du Pont de Nemours and Company
    Inventor: Quinn Qun Zhu
  • BIOCATALYSTS AND METHODS FOR THE SYNTHESIS OF (S)-3-(1-AMINOETHYL)-PHENOL
    Publication number: 20140356944
    Abstract: The present disclosure provides engineered transaminase polypeptides having improved properties as compared to naturally occurring transaminases including the ability of converting the substrate, 3?-hydroxyacetophenone to (S)-3-(1-aminoethyl)-phenol in enantiomeric excess and high percentage conversion. Also provided are polynucleotides encoding the engineered transaminases, host cells capable of expressing the engineered transaminases, and methods of using the engineered transaminases to synthesize (S)-3-(1-aminoethyl)-phenol and related compounds useful in the production of active pharmaceutical ingredients.
    Type: Application
    Filed: August 19, 2014
    Publication date: December 4, 2014
    Inventors: Fabien Louis Cabirol, Anupam Gohel, Seong Ho Oh, Derek J. Smith, Brian Wong, James J. Lalonde
  • ORGANISMS FOR THE PRODUCTION OF CYCLOHEXANONE
    Publication number: 20140356919
    Abstract: A non-naturally occurring microbial organism has cyclohexanone pathways that include at least one exogenous nucleic acid encoding a cyclohexanone pathway enzyme. A pathway includes a 2-ketocyclohexane-1-carboxyl-CoA hydrolase (acting on C—C bond), a 2-ketocyclohexane-1-carboxylate decarboxylase and an enzyme selected from a 2-ketocyclohexane-1-carboxyl-CoA hydrolase (acting on thioester), a 2-ketocyclohexane-1-carboxyl-CoA transferase, and a 2-ketocyclohexane-1-carboxyl-CoA synthetase.
    Type: Application
    Filed: February 4, 2014
    Publication date: December 4, 2014
    Inventors: Robin E. Osterhout, Anthony P. Burgard, Mark J. Burk, Priti Pharkya
  • GENES CONFERRING TOLERANCE TO ETHANOL AND HIGH TEMPERATURE FOR YEASTS
    Publication number: 20140356879
    Abstract: Methods of identifying genes conferring ethanol tolerance in yeasts, genes that confer ethanol tolerance, and mutant strains used to identify such genes are described. A gene herein designated HpETT1 was isolated from the yeast Hansenula polymorpha. Expression of HpETT1 in an ethanol sensitive mutant H. polymorpha strain designated 7E complimented ethanol sensitivity of the mutant. When multiple copies of the HpETT1 were integrated into the genome and overexpressed, the transformed strain demonstrated approximately 10-fold greater resistance to ethanol and resistance to the protein misfolding agent AZC. Expression of HpETT1 also increased ethanol tolerance in Saccharomyces cerevisiae. HpEtt1 has 39% sequence identity to a previously identified protein from S. cerevisiae denoted MPE1, however, the MPE1 gene does not confer ethanol resistance to the 7E mutant.
    Type: Application
    Filed: January 11, 2013
    Publication date: December 4, 2014
    Inventors: Charles Abbas, Andriy Sibirny, Andriy Voronovsky, Olena Ishchuk
  • NOVEL SERINE PROTEASE VARIANTS
    Publication number: 20140356347
    Abstract: The present disclosure provides novel variants of enzymes exhibiting serine protease activity; nucleic acid molecules encoding said proteases, vectors, host cells containing the nucleic acids and methods for preparation and producing such enzymes; compositions and complexes comprising at least one of the proteases; and methods for using such enzymes as a part of an immunoprotease, in particular for the treatment of cancer.
    Type: Application
    Filed: September 21, 2012
    Publication date: December 4, 2014
    Inventors: Stefan Barth, Sonja Schiffer, Grit Hehmann-Titt
  • CELL-BASED PRODUCTION OF NONULOSONATES
    Publication number: 20140356912
    Abstract: The present invention relates to the the cell-based production of bacterial nonulosonates and their biosynthetic precursors. Specifically, the present invention provides recombinant cells for the production of pseudaminic acid, legionaminic acid, UDP-2,4-diacetamido-2,4,6-trideoxy-?-L-altropyranose, and UDP-2,4-diacetamido-2,4,6-trideoxy-?-D-glucopyranose. Methods for producing the sugars are also provided.
    Type: Application
    Filed: July 28, 2014
    Publication date: December 4, 2014
    Inventors: Christopher N. Boddy, Susan M. Logan, Benjamin R. Lundgren, Ian C. Schoenhofen, Dennis M. Whitfield
  • TOOLS AND METHODS FOR EXPRESSION OF MEMBRANE PROTEINS
    Publication number: 20140357521
    Abstract: The disclosure relates cells or cellular systems that express both a membrane protein and a binding domain directed to the membrane protein. Also, methods are provided that use such cells or cellular systems to produce higher amounts of the membrane proteins. Further, the cells or cellular systems can be used as tools for the structural and functional characterization of membrane proteins, as well as for screening and drug discovery efforts targeting membrane proteins.
    Type: Application
    Filed: January 21, 2013
    Publication date: December 4, 2014
    Inventors: Jan Steyaert, Nico Callewaert, Toon Laeremans, Els Pardon, Kristof Vandewalle, Katrien Claes
  • BIOLOGICALLY ACTIVE PROTEINS HAVING INCREASED IN VIVO AND/OR IN VITRO STABILITY
    Publication number: 20140356326
    Abstract: The present invention provides unstructured recombinant polymers (URPs) and proteins containing one or more of the URPs. The present invention also provides microproteins, toxins and other related proteinaceous entities, as well as genetic packages displaying these entities. The present invention also provides recombinant polypeptides including vectors encoding the subject proteinaceous entities, as well as host cells comprising the vectors. The subject compositions have a variety of utilities including a range of pharmaceutical applications.
    Type: Application
    Filed: December 10, 2013
    Publication date: December 4, 2014
    Applicant: Amunix Operating Inc.
    Inventors: Volker Schellenberger, Willem P. Stemmer, Chia-Wei Wang, Michael D. Scholle, Mikhail Popkov, Nathaniel C. Gordon, Andreas Crameri
  • FUSION OF BIOACTIVE MOLECULES
    Publication number: 20140356478
    Abstract: Fusion-proteins containing an enzyme, preferably a feed or food enzyme, coupled to a gut surface-binding domain are presented. The fusion-proteins can be used to promote feed utilization in animals. In a particular example, a Fusion enzyme according to the invention comprising a gut-surface-binding polypeptide segment linked to a phytase show an increased resident time in the gut, which leads to an increased amount of time given to the enzyme to catalyse the corresponding reaction which finally leads to improved feed utilisation.
    Type: Application
    Filed: June 11, 2012
    Publication date: December 4, 2014
    Applicant: NOVOZYMES A/S
    Inventors: Ghislain Schyns, Stephane Duval, Aurelie Brelin
  • ANTIBODIES SPECIFIC FOR TROP-2 AND THEIR USES
    Publication number: 20140357844
    Abstract: The present invention provides antibodies that specifically bind to trophoblast cell-surface antigen-2 (Trop-2). The invention further provides antibody conjugates comprising such antibodies, antibody encoding nucleic acids, and methods of obtaining such antibodies. The invention further relates to therapeutic methods for use of these antibodies and Trop-2 antibody conjugates for the treatment of a condition associated with Trop-2 expression (e.g., cancer), such as colon, esophageal, gastric, head and neck, lung, ovarian, or pancreatic cancer.
    Type: Application
    Filed: August 15, 2014
    Publication date: December 4, 2014
    Inventors: Shu-Hui LIU, Wei-Hsien HO, Pavel STROP, Magdalena Grazyna DORYWALSKA, Arvind RAJPAL, David Louis SHELTON, Thomas-Toan TRAN
  • NEUTRALIZING GP41 ANTIBODIES AND THEIR USE
    Publication number: 20140348785
    Abstract: Monoclonal neutralizing antibodies are disclosed that specifically bind to the HIV-1 gp41 membrane-proximal external region (MPER). Also disclosed are compositions including the disclosed antibodies that specifically bind gp41, nucleic acids encoding these antibodies, expression vectors including the nucleic acids, and isolated host cells that express the nucleic acids. The antibodies and compositions disclosed herein can be used for detecting the presence of HIV-1 in a biological sample, or detecting an HIV-1 infection or diagnosing AIDS in a subject. In additional, the broad neutralization breadth of the disclosed antibodies makes them ideal for treating a subject with an HIV infection. Thus, disclosed are methods of treating and/or preventing HIV infection.
    Type: Application
    Filed: November 7, 2012
    Publication date: November 27, 2014
    Inventors: Mark Connors, Jinghe Huang, Leo B. Laub, Peter Kwong, Gary Nabel, John R. Mascola, Baoshan Zhang, Rebecca S. Rudicell, Ivelin Georgiev, Yongping Yang, Jiang Zhu, Gilad Ofek
  • ENHANCED PRODUCTION AND PURIFICATION OF A NATURAL HIGH INTENSITY SWEETENER
    Publication number: 20140350223
    Abstract: Recombinant P. pastoris producing natural sweet proteins and methods for engineering these recombinant yeast are described. Methods for enhancing foreign protein production in yeast fermentation and improved methods for purification of foreign proteins produced in yeast fermentation are presented.
    Type: Application
    Filed: April 17, 2014
    Publication date: November 27, 2014
    Applicant: Tate & Lyle Technology Limited
    Inventors: Alfred Carlson, Richard W. Armentrout, Timothy Peter Ellis
  • Lipase Variants and Polynucleotides Encoding Same
    Publication number: 20140349372
    Abstract: The present invention relates to lipase variants and methods of obtaining them. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
    Type: Application
    Filed: January 25, 2013
    Publication date: November 27, 2014
    Applicant: Novozymes A/S
    Inventors: Luise Erlandsen, Carsten Hoerslev Hansen, Jesper Vind, Allan Svendsen, Carsten Peter Sonksen
  • ENGINEERING PLANTS FOR EFFICIENT UPTAKE AND UTILIZATION OF UREA TO IMPROVE CROP PRODUCTION
    Publication number: 20140351998
    Abstract: The present invention provides polynucleotides and related polypeptides related to urea uptake. The invention provides genomic sequences for urea transporter, urease and glutamine synthetase genes. Urea transporters, urease and glutamine synthetase are responsible for controlling nitrogen utilization efficiency in plants. Urea transporter, urease or glutamine synthetase sequences are provided for improving grain yield and plant growth. The invention further provides recombinant expression cassettes, host cells and transgenic plants.
    Type: Application
    Filed: November 19, 2013
    Publication date: November 27, 2014
    Applicant: PIONEER HI BRED INTERNATIONAL INC
    Inventors: RAJEEV GUPTA, Zhenglin Hou, Dale F. Loussaert, Bo Shen, Lawrence Kent Wood
  • Sclareol and labdenediol diphosphate synthase polypeptides, encoding nucleic acid molecules and uses thereof
    Publication number: 20140349352
    Abstract: Provided are labdenediol diphosphate synthase polypeptides, sclareol synthase polypeptides, nucleic acid molecules encoding the labdenediol diphosphate synthase polypeptides and sclareol synthase polypeptides, and methods of using the labdenediol diphosphate synthase polypeptides, sclareol synthase polypeptides. Also provided are methods for producing labdenediol diphosphate, sclareol and (?)-ambroxide.
    Type: Application
    Filed: July 30, 2013
    Publication date: November 27, 2014
    Applicant: Allylix, Inc.
    Inventors: Grace Eunyoung Park, Bryan N. Julien, Richard Burlingame
  • COMPANION DIAGNOSTIC FOR ANTI-HYALURONAN AGENT THERAPY AND METHODS OF USE THEREOF
    Publication number: 20140348817
    Abstract: Methods and diagnostic agents for identification of subjects for cancer treatment with an anti-hyaluronan agent, such as a hyaluronan-degrading enzyme, are provided. Diagnostic agents for the detection and quantification of hyaluronan in a biological sample and monitoring cancer treatment with an anti-hyaluronan agent, for example a hyaluronan-degrading enzyme, are provided. Combinations and kits for use in practicing the methods also are provided.
    Type: Application
    Filed: August 14, 2014
    Publication date: November 27, 2014
    Inventors: Ping Jiang, H. Michael Shepard, Lei Huang
  • BETA-ALANINE/ALPHA-KETOGLUTARATE AMINOTRANSFERASE FOR 3-HYDROXYPROPIONIC ACID PRODUCTION
    Publication number: 20140349376
    Abstract: The present disclosure provides novel beta-alanine/alpha ketoglutarate aminotransferase nucleic acid and protein sequences having increased biological activity. Also provided are cells containing such enzymes, as well as methods of their use, for example to produce malonyl semialdehyde and downstream products thereof, such as 3-hydroxypropionic acid and derivatives thereof.
    Type: Application
    Filed: September 12, 2011
    Publication date: November 27, 2014
    Inventors: Holly Jean Jessen, Hans H. Liao, Steven John Gort, Olga V. Selifonova
  • ANTI-CD40 ANTIBODIES AND METHODS OF USE
    Publication number: 20140349395
    Abstract: The present invention provides high affinity anti-CD40 monoclonal antibodies and related compositions, which may be used in any of a variety of therapeutic methods for the treatment of cancer and other diseases.
    Type: Application
    Filed: June 5, 2014
    Publication date: November 27, 2014
    Applicant: Apexigen, Inc.
    Inventors: Yongke ZHANG, Guo-Liang YU, Weimin ZHU
  • Metabolically engineered cells for the production of resveratrol or an oligomeric or glycosidically-bound derivative thereof
    Patent number: 8895287
    Abstract: A recombinant micro-organism producing resveratrol by a pathway in which phenylalanine ammonia lyase (PAL) produces trans-cinnamic acid from phenylalanine, cinnamate 4-hydroxylase (C4H) produces 4-coumaric acid from said trans-cinnamic acid, 4-coumarate-CoA ligase (4CL) produces 4-coumaroyl CoA from said 4-coumaric acid, and resveratrol synthase (VST) produces said resveratrol from said 4-coumaroyl CoA, or in which L-phenylalanine- or tyrosine-ammonia lyase (PAL/TAL) produces 4-coumaric acid, 4-coumarate-CoA ligase (4CL) produces 4-coumaroyl CoA from said 4-coumaric acid, and resveratrol synthase (VST) produces said resveratrol from said 4-coumaroyl CoA. The micro-organism may be a yeast, fungus or bacterium including Saccharomyces cerevisiae, E. coli, Lactococcus lactis, Aspergillus niger, or Aspergillus oryzae.
    Type: Grant
    Filed: February 21, 2006
    Date of Patent: November 25, 2014
    Assignee: Evolva SA
    Inventors: Michael Katz, Hans Peter Smits, Jochen Förster, Jens Bredal Nielsen
  • BIOCATALYSTS AND METHODS FOR THE SYNTHESIS OF SUBSTITUTED LACTAMS
    Publication number: 20140342412
    Abstract: The present disclosure relates to transaminase polypeptides capable of aminating a dicarbonyl substrate, and polynucleotides, vectors, host cells, and methods of making and using the transaminase polypeptides.
    Type: Application
    Filed: September 7, 2012
    Publication date: November 20, 2014
    Applicant: CODEXIS, INC.
    Inventors: Fabien Cabirol, Haibin Chen, Anupam Gohel, Paulina Salim, Derek Smith, Jacob Janey, Birgit Kosjek, Weng Lin Tang, Helen Hsieh, Son Pham
  • VASCULAR ENDOTHELIAL GROWTH FACTOR 2
    Publication number: 20140342405
    Abstract: Disclosed are human VEGF-2 antibodies, antibody fragments, or variants thereof. Also provided are processes for producing such antibodies. The present invention relates to methods and compositions for preventing, treating or ameliorating a disease or disorder comprising administering to an animal, preferably a human, an effective amount of one or more VEGF-2 antibodies or fragments or variants thereof.
    Type: Application
    Filed: May 15, 2014
    Publication date: November 20, 2014
    Applicant: Human Genome Sciences, Inc.
    Inventors: Craig Rosen, Vivian Albert, Steven M. Ruben, Ruth Wager
  • Polypeptides Having Cellobiohydrolase Activity and Polynucleotides Encoding Same
    Publication number: 20140342410
    Abstract: The present invention relates to isolated polypeptides having cellobiohydrolase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Application
    Filed: May 14, 2014
    Publication date: November 20, 2014
    Applicant: Novozymes Inc.
    Inventor: Nikolaj Spodsberg
  • RNA-Guided Human Genome Engineering
    Publication number: 20140342456
    Abstract: A method of altering a eukaryotic cell is provided including transfecting the eukaryotic cell with a nucleic acid encoding RNA complementary to genomic DNA of the eukaryotic cell, transfecting the eukaryotic cell with a nucleic acid encoding an enzyme that interacts with the RNA and cleaves the genomic DNA in a site specific manner, wherein the cell expresses the RNA and the enzyme, the RNA binds to complementary genomic DNA and the enzyme cleaves the genomic DNA in a site specific manner.
    Type: Application
    Filed: June 30, 2014
    Publication date: November 20, 2014
    Inventors: Prashant G. MALI, George M. CHURCH, Luhan Yang
  • GLUCOAMYLASE VARIANTS AND POLYNUCLEOTIDES ENCODING SAME
    Publication number: 20140342422
    Abstract: The present invention relates to glucoamylase variants having reduced sensitivity to protease nicking. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
    Type: Application
    Filed: September 5, 2012
    Publication date: November 20, 2014
    Applicant: NOVOZYMES A/S
    Inventors: Tomoko Matsui, Suzanne Clark
  • Polypeptides Having Xylanase Activity and Polynucleotides Encoding Same
    Publication number: 20140342408
    Abstract: Provided are isolated polypeptides having xylanase activity, catalytic domains and cellulose binding domains, and polynucleotides encoding the polypeptides, catalytic domains or cellulose binding domains. Also provided are nucleic acid constructs, vectors and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains or cellulose binding domains.
    Type: Application
    Filed: November 22, 2012
    Publication date: November 20, 2014
    Inventors: Yu Zhang, Lan Tang, Junxin Duan, Ye Liu