Fungi Patents (Class 435/254.1)
  • Patent number: 10214630
    Abstract: A crystalline pigment or colorant composition having high color intensity and/or low sugar content, and methods and processes of preparation. The composition may comprise purified fruit and/or vegetable color juices.
    Type: Grant
    Filed: March 3, 2017
    Date of Patent: February 26, 2019
    Inventors: Joseph Rossi, Sundeep Kattamuri
  • Patent number: 10167446
    Abstract: A method for cultivating antrodia cinnamomea includes transplanting bacterial strains of antrodia cinnamomea into a petri dish receiving culture ingredients. The bacterial strains of antrodia cinnamomea are transferred into a culture container after mycelia of the bacterial strains of antrodia cinnamomea have grown to a predetermined area. The culture container receives a culture fluid. A formula composition of a culture medium for cultivating antrodia cinnamomea is added into the culture container after the mycelia of the bacterial strains of antrodia cinnamomea have grown in the culture container. The formula composition of the culture medium for cultivating antrodia cinnamomea includes growth basal ingredients and cultivating basal ingredients. The growth basal ingredients includes hydroxyhopanone.
    Type: Grant
    Filed: June 1, 2015
    Date of Patent: January 1, 2019
    Assignee: CHENG-LIN AGRICULTURAL BIOTECHNOLOGY CO. LTD.
    Inventor: Min-Shih Chen
  • Patent number: 10154627
    Abstract: A growth medium formed as an inoculum including a preselected fungus and a nutrient material capable of being digested by the fungus is placed in or on a tool formed of a material capable of being at least partially consumed by the fungus. The tool may define a cavity of predetermined shape for the growth medium or the tool may form a scaffolding on which the growth medium grows into the final product taking on the shape of the tool.
    Type: Grant
    Filed: April 5, 2013
    Date of Patent: December 18, 2018
    Assignee: ECOVATIVE DESIGN LLC
    Inventors: Gavin McIntyre, Eben Bayer, Amanda Palazzolo
  • Patent number: 9999219
    Abstract: A paraffinic spray oil and a method of using the spray oil for controlling turfgrass pests is disclosed. The spray oil comprises paraffinic oil and a quick break emulsifier, which is formulated as an oil-in-water (O/W) emulsion for use. The paraffinic oil and emulsifier are present in a weight ratio ranging from about 95:5 to about 99.95:0.05, and preferably from about 98.5:1.5 to about 99.9:0.1. When applied to turfgrass, the O/W emulsion quickly releases the oil phase upon application to the turfgrass to contact pests thereon. When provided at sufficient paraffinic oil dosages, generally at least about 0.5 gal oil/acre and preferably in the range of about 0.5 gal/acre to about 60 gal/acre, the spray oil is effective in controlling a variety of turfgrass pests, particularly insect and fungal pests, with little or no phytotoxic effects. Further, use of the spray oil as indicated for controlling turfgrass pests also enhances the growth of turfgrass.
    Type: Grant
    Filed: February 17, 2017
    Date of Patent: June 19, 2018
    Assignee: Suncor Energy Inc.
    Inventor: Michael Fefer
  • Patent number: 9963829
    Abstract: An invention in the field of papermaking that relates in particular to sizing paper. Problems with state of the art methods are that the sizing chemicals used are typically expensive; may be available only in limited supply; are produced using methods that are damaging to the environment; and whose production is far from carbon-neutral. It is an object of the present invention to provide an alternative to the methods of the prior art and to overcome one or more of the above mentioned disadvantages.
    Type: Grant
    Filed: April 14, 2016
    Date of Patent: May 8, 2018
    Assignee: TECHNISCHE UNIVERSITEIT DELFT
    Inventors: Marinus Cornelis Maria Van Loosdrecht, Lin Yuemei, Tommaso Lotti
  • Patent number: 9920303
    Abstract: The present invention relates to Brassica plants comprising mutant FAD3 alleles, FAD3 nucleic acid sequences and proteins, as well as methods for generating and identifying said plants and alleles, which can be used to obtain seed oil with a reduced alpha-linolenic acid content.
    Type: Grant
    Filed: November 19, 2010
    Date of Patent: March 20, 2018
    Assignee: BAYER CROPSCIENCE NV
    Inventors: Benjamin Laga, Peter Denolf
  • Patent number: 9695454
    Abstract: Described herein are compositions including filamentous fungal cells, such as Trichoderma fungal cells, having reduced protease activity and expressing fucosylation pathway. Further described herein are methods for producing a glycoprotein having fucosylated N-glycan, using genetically modified filamentous fungal cells, for example, Trichoderma fungal cells, as the expression system.
    Type: Grant
    Filed: May 23, 2013
    Date of Patent: July 4, 2017
    Assignee: GLYKOS FINLAND OY
    Inventors: Jari Natunen, Ann Westerholm-Parvinen, Hanna Salo, Jussi Joensuu, Heli Viskari, Christopher Landowski, Anne Huuskonen, Anne Kanerva, Anna-Liisa Hanninen, Noora Salovuori, Merja Penttila, Juhani Saarinen, Markku Saloheimo
  • Patent number: 9695429
    Abstract: The present invention relates to methods for producing a biological substance, comprising: (a) cultivating a fungal host cell in a medium conducive for the production of the biological substance, wherein the fungal host cell comprises a first nucleic acid sequence encoding the biological substance operably linked to a second nucleic acid sequence comprising a promoter variant selected from the group consisting of SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, and SEQ ID NO: 12; and a subsequence thereof; and hybrid and tandem promoters thereof; and (b) isolating the biological substance from the cultivation medium. The present invention also relates to the isolated promoter variants and to constructs, vectors, and fungal host cells comprising the promoter variants operably linked to nucleic acid sequences encoding biological substances.
    Type: Grant
    Filed: September 10, 2015
    Date of Patent: July 4, 2017
    Assignee: NOVOZYMES, INC.
    Inventors: Debbie Yaver, Peter Nham
  • Patent number: 9643959
    Abstract: The present invention relates to (E)-ethyl 2-(2-methyl-3-((2-(naphtho[2,1-b]furan-2-carbonyl)hydrazono)methyl)-1H-indol-1-yl)acetate as a novel tubulin polymerization inhibitor and a method for synthesizing the same. The compound of the present invention can inhibit mitosis and induce apoptosis and thus be used as an anticancer agent, by binding to tubulin to inhibit microtube polymerization. According to the synthesis method of the present invention, the reaction is simplified and the efficiency is 60% or higher, leading to a very high yield, thereby providing an effective synthesis method.
    Type: Grant
    Filed: April 22, 2014
    Date of Patent: May 9, 2017
    Assignee: Korea Research Institute of Bioscience and Biotechnology
    Inventors: Bo Yeon Kim, Thimmegowda N.R., Sun Ok Kim, Nak Kyun Soung, Krisada Sakchaisri, InJa Ryoo, Chan-Mi Park, Jong Seog Ahn, Jae-Hyuk Jang
  • Patent number: 9622428
    Abstract: A hybrid mushroom culture of Agaricus bisporus, designated as strain J11500, includes a representative culture of the strain, which has been deposited under NRRL Accession No. 50895. A method of producing a hybrid mushroom culture of Agaricus bisporus comprising: mating a homokaryotic line J10102-s69 with a homokaryotic line OWNC. Additionally, mushrooms, parts of the culture and products incorporating the culture are provided.
    Type: Grant
    Filed: January 31, 2014
    Date of Patent: April 18, 2017
    Assignee: Sylvan America, Inc.
    Inventors: Richard W. Kerrigan, Mark P. Wach, Michelle E. Schultz
  • Patent number: 9301985
    Abstract: The present invention provides a therapeutic agent for dementia comprising a pharmaceutically active substance derived from Hericium ramosum.
    Type: Grant
    Filed: March 13, 2013
    Date of Patent: April 5, 2016
    Assignee: KIBUN FOODS INC.
    Inventors: Kohei Suruga, Yasuhiro Komatsu, Kazunari Kadokura, Yoshihiro Sekino, Kenichi Mishima, Michihiro Fujiwara
  • Patent number: 9044467
    Abstract: A method for assisting an anti-cancer drug comprising administrating an effective amount of Antrodia camphorata fermentation solution to improve life quality of patient suffering from cancers after chemotherapy is provided.
    Type: Grant
    Filed: August 24, 2012
    Date of Patent: June 2, 2015
    Assignee: NEW BELLUS ENTERPRISES CO., LTD.
    Inventors: Wen-Li Yeh, Chun-Chih Huang
  • Publication number: 20150132740
    Abstract: A concentration agent for microorganisms is provided that contains both lanthanum and carbonate. Additionally, articles that include the concentration agent and methods of concentrating a microorganism using the concentration agent are provided.
    Type: Application
    Filed: May 23, 2013
    Publication date: May 14, 2015
    Inventors: Manjiri T. Kshirsagar, Evan Koon Lun Yuuji Hajime, Andrew W. Rabins
  • Patent number: 9029121
    Abstract: A method for the biological decomposition of organic compounds of the group of hydrocarbons, fats, oils, waxes, and derivatives thereof as well as mixtures thereof in media polluted therewith, in the presence of at least one dispersant, wherein the medium polluted with the pollutants is treated with at least one dispersant and with microorganisms.
    Type: Grant
    Filed: June 15, 2010
    Date of Patent: May 12, 2015
    Assignee: Total Marketing Services
    Inventors: Ursula Fritzmeier, Robert Mallee, Achim Wiessler
  • Publication number: 20150125923
    Abstract: The present invention generally relates to compositions and methods of delivering substances in a dry mode, wherein the compositions include a surface layer disposed on the outer surface of the composition that is permeable to carbon dioxide and oxygen. The compositions may be used to deliver microorganisms to remove contaminates, such as oil, chemical, waste, or sewage, from soil, water, or air. In other embodiments, the compositions can also be used for delivering liquid food, liquid food additives, liquid biotech agricultural ingredients, conventional liquid agricultural ingredients, liquid human wellness and dietary supplements, and liquid fragrances and beauty products.
    Type: Application
    Filed: October 5, 2011
    Publication date: May 7, 2015
    Applicant: DryLet LLC
    Inventors: Ramiro Trevino, Steven R. Ellis
  • Publication number: 20150118753
    Abstract: A method and system for achieving a gas-liquid mass transfer includes delivering into a compartment of a container a liquid, the liquid having an exposed top surface disposed within the compartment. A stream of a gas is passed over the top surface of the liquid so that the stream of gas produces turbulence on the top surface that is sufficient to achieve the gas-liquid mass transfer. In one embodiment the liquid is a culture that includes cells or microorganisms and the mass transfer functions to oxygenate the culture sufficient to sustain the cells or microorganisms.
    Type: Application
    Filed: March 15, 2013
    Publication date: April 30, 2015
    Inventors: Christopher D. Brau, Nephi D. Jones, Benjamin R. Madsen, Michael E. Goodwin
  • Patent number: 9017988
    Abstract: A hybrid mushroom culture of Agaricus bisporus, designated as strain B14528, includes a representative culture of the strain, which has been deposited under NRRL Accession No. 50900. A method of producing a hybrid mushroom culture of Agaricus bisporus comprising: mating a homokaryotic line J12998-s39 with a homokaryotic line BW-s191. Additionally, mushrooms, parts of the culture and products incorporating the culture are provided.
    Type: Grant
    Filed: February 21, 2014
    Date of Patent: April 28, 2015
    Assignee: Sylvan America, Inc.
    Inventors: Richard W. Kerrigan, Mark P. Wach, Michelle E. Schultz
  • Publication number: 20150104868
    Abstract: The present invention relates to methods of using meso-1,2,3,4-tetrahydroxybutane for the maintenance and/or improvement of biological cell function and activity, and for the prevention of improper cell functioning or cell death, in vitro, ex vivo, and in vivo over time and/or during exposure to stress. Meso-1,2,3,4-tetrahydroxybutane can be used to promote cell survival and as a cell protection agent, to increase cell viability, and to improve conversion of progenitor or stem cells to mature cells, whether in vitro, in vivo, ex-vivo, or transplanted.
    Type: Application
    Filed: April 19, 2013
    Publication date: April 16, 2015
    Applicant: CARGILL, INC.
    Inventors: Alvin Berger, Aalt Bast, Petrus Wilhelmus Hubertus De Cock, Gerardus Johannes Martinus Den Hartog, Jean-Claude Marie-Pierre Ghislain De Troostembergh
  • Patent number: 8999687
    Abstract: The composite material is comprised of a substrate of discrete particles and a network of interconnected mycelia cells bonding the discrete particles together. The mycelia cells are selected from the group consisting of at least one of Agrocybe brasiliensi, Flammulina velutipes, Hypholomoa capnoides, Hypholoma sublaterium, Morchella angusticeps, Macrolepiota procera and Coprinus comatus. The fungus digests the nutrient material over a period of time sufficient to grow hyphae and to allow the hyphae to form a network of interconnected mycelia cells through and around the discrete particles thereby bonding the discrete particles together to form a self-supporting composite material.
    Type: Grant
    Filed: May 29, 2012
    Date of Patent: April 7, 2015
    Assignee: Rensselaer Polytechnic Institute
    Inventors: Eben Bayer, Gavin McIntyre, Burt L. Swersey
  • Patent number: 8986974
    Abstract: The present invention relates to mutants of a parent Trichoderma strain, comprising a polynucleotide encoding a polypeptide and one or more (several) genes selected from the group consisting of a first subtilisin-like serine protease gene, a first aspartic protease gene, a trypsin-like serine protease gene, a second subtilisin-like serine protease gene, and a second aspartic protease gene, wherein the one or more (several) genes are modified rendering the mutant strain deficient in the production of one or more (several) enzymes selected from the group consisting of a first subtilisin-like serine protease, a first aspartic protease, a trypsin-like serine protease, a second subtilisin-like serine protease, and a second aspartic protease, respectively, compared to the parent Trichoderma strain when cultivated under identical conditions. The present invention also relates to methods of producing a polypeptide in such mutants and methods for producing such mutants.
    Type: Grant
    Filed: December 17, 2010
    Date of Patent: March 24, 2015
    Assignee: Novozymes, Inc.
    Inventors: Suchindra Maiyuran, Abigail Jang, Kimberly Brown, Sandra Merino, Jeffrey Shasky, Eric Abbate
  • Patent number: 8975064
    Abstract: The present invention relates to fungal cells and fungi which synthesize hyaluronan and to methods for preparing such fungi, and also to methods for preparing hyaluronan with the aid of these fungal cells or fungi. Furthermore, the present invention relates to the use of fungi for preparing hyaluronan and to food or feed which comprises hyaluronan.
    Type: Grant
    Filed: February 22, 2006
    Date of Patent: March 10, 2015
    Assignee: Bayer Cropscience AG
    Inventor: Martin Quanz
  • Patent number: 8975489
    Abstract: The invention provides isolated fungal endophytes and synthetic combinations thereof with host grass plants. Methods for inoculating grass plant with the endophytes, for propagating the grass-endophyte combinations, and for producing feeds and biofuels from grass-endophyte combinations are also provided.
    Type: Grant
    Filed: November 29, 2011
    Date of Patent: March 10, 2015
    Assignee: The Samuel Roberts Noble Foundation
    Inventor: Kelly Craven
  • Patent number: 8969064
    Abstract: The present invention provides a method for producing a desired protein (such as a desired heterologous protein) comprising: (a) providing a host cell comprising a first recombinant gene encoding a protein comprising the sequence of a first chaperone protein, a second recombinant gene encoding a protein comprising the sequence of a second chaperone protein and a third gene, such as a third recombinant gene, encoding a desired protein (such as a desired heterologous protein), wherein the first and second chaperones are different; and (b) culturing the host cell in a culture medium to obtain expression of the first, second and third genes.
    Type: Grant
    Filed: May 17, 2012
    Date of Patent: March 3, 2015
    Assignee: Novozymes Biopharma DK A/S
    Inventors: Christopher John Arthur Finnis, Darrell Sleep, Gillian Shuttleworth
  • Patent number: 8969011
    Abstract: A process for capturing or concentrating microorganisms for detection or assay comprises (a) providing a concentration device comprising a sintered porous polymer matrix comprising at least one concentration agent that comprises an amorphous metal silicate and that has a surface composition having a metal atom to silicon atom ratio of less than or equal to 0.5, as determined by X-ray photoelectron spectroscopy (XPS); (b) providing a sample comprising at least one microorganism strain; and (c) contacting the concentration device with the sample such that at least a portion of the at least one microorganism strain is bound to or captured by the concentration device.
    Type: Grant
    Filed: March 22, 2010
    Date of Patent: March 3, 2015
    Assignee: 3M Innovative Properties Company
    Inventors: Manjiri T. Kshirsagar, Andrew W. Rabins
  • Publication number: 20150050244
    Abstract: The present invention provides a strain of entomopathogenic Beauveria bassiana, compositions comprising the entomopathogenic fungi strain or metabolites of the strain, and the use of the entomopathogenic fungi strain and compositions as biological control agents. Methods for the biological control of phytopathogenic insects using an entomopathogenic Beauveria bassiana fungi strain or one or more metabolites thereof, optionally together with other entomopathogenic fungi including fungi selected from strains of Lecanicillium spp., Paecilomyces fumosoroseus, and compositions comprising said fungi or metabolites thereof are also provided.
    Type: Application
    Filed: May 5, 2014
    Publication date: February 19, 2015
    Applicant: Greentide Limited, a New Zealand Company
    Inventor: Stephen Reynold FORD
  • Patent number: 8945886
    Abstract: A method for producing a polyunsaturated fatty acid (PUFA) or a lipid containing a PUFA, a microbial cell containing a PUFA, and use of the microbial cell are provided. A method for producing a polyunsaturated fatty acid (PUFA) or a lipid containing a PUFA including culture of a microorganism capable of producing arachidonic acid (ARA) and/or dihomo-gamma-linolenic acid (DGLA) is provided, the method including at least one of the following steps: (a) adding an organic acid in an amount of 0.01 to 5 w/v % to a culture medium after the beginning of main culture; (b) increasing the pH of the culture medium to a range effective for culture after the beginning of the main culture; and (c) adding a metal sulfate in an amount of 0.01 to 0.5 w/w % to the main culture medium.
    Type: Grant
    Filed: January 15, 2008
    Date of Patent: February 3, 2015
    Assignee: Suntory Holdings Limited
    Inventors: Kenji Katano, Hiroshi Kawashima
  • Patent number: 8932825
    Abstract: The present invention relates to host cells having modified lipid-linked oligosaccharides which may be modified further by heterologous expression of a set of glycosyltransferases, sugar transporters and mannosidases to become host-strains for the production of mammalian, e.g., human therapeutic glycoproteins. The process provides an engineered host cell which can be used to express and target any desirable gene(s) involved in glycosylation. Host cells with modified lipid-linked oligosaccharides are created or selected. N-glycans made in the engineered host cells have a GlcNAcMan3GlcNAc2 core structure which may then be modified further by heterologous expression of one or more enzymes, e.g., glycosyl-transferases, sugar transporters and mannosidases, to yield human-like glycoproteins. For the production of therapeutic proteins, this method may be adapted to engineer cell lines in which any desired glycosylation structure may be obtained.
    Type: Grant
    Filed: December 24, 2002
    Date of Patent: January 13, 2015
    Assignee: GlycoFi Inc.
    Inventors: Stefan Wildt, Robert Gordon Miele, Juergen Hermann Nett, Robert C. Davidson
  • Patent number: 8927254
    Abstract: Provided herein are methods for transforming a Pyrococcus furiosus with a polynucleotide. In one embodiment, the method includes contacting a P. furiosus with a polynucleotide under conditions suitable for uptake of the polynucleotide by the P. furiosus, and identifying transformants at a frequency of, for instance, at least 103 transformants per microgram DNA. Also provided are isolated Pyrococcus furiosus having the characteristics of Pyrococcus furiosus COM1, and plasmids that include an origin of replication that functions in a Pyrococcus furiosus. The plasmid is stable in a recipient P. furiosus without selection for more than 100 generations and is structurally unchanged after replication in P. furiosus for more than 100 generations.
    Type: Grant
    Filed: September 29, 2011
    Date of Patent: January 6, 2015
    Assignee: University of Georgia Research Foundation, Inc.
    Inventors: Gina L. Lipscomb, Joel Andrew Farkas, Michael W. W. Adams, Janet Westpheling
  • Patent number: 8927256
    Abstract: The present invention demonstrates the utility of carbonic acid amides such as urea or its derivatives, carbamates, carbodiimides & thiocarbamides as nitrogenous supplements in fermentation media for production of recombinant proteins to achieve enhanced bioconversion rates and peptides like insulin and insulin analogues, exendin and enzymes such as lipase using methanol inducible fungal expression systems such as Pichia.
    Type: Grant
    Filed: February 5, 2009
    Date of Patent: January 6, 2015
    Assignee: Biocon Limited
    Inventors: Sanjay Tiwari, Mukesh Babuappa Patale, Saurabh Garg, Mayank Kumar Garg, Sulekha Joshi, Chittnalli Ramegowda Naveen Kumar, Bimal Kumar, Anuj Goel, Harish Iyer
  • Publication number: 20150004656
    Abstract: The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Application
    Filed: September 15, 2014
    Publication date: January 1, 2015
    Inventors: Lan Tang, Ye Liu, Junxin Duan, Yu Zhang, Christian Joergensen, Randall Kramer
  • Patent number: 8921067
    Abstract: A thin film culture device for detecting yeast and mold microorganisms in a sample is provided. The culture device comprises a body comprising a self-supporting substrate having a first major surface and a second major surface; a first adhesive composition disposed on a portion of the first major surface of the substrate; a substantially dry, cold-water-soluble first hydrogel-forming composition adhered to the first adhesive composition; and a plurality of indicator agents. The plurality of indicator agents comprises three indicator agents for detecting distinct glycosidase enzyme activities, an indicator agent for detecting an alkyl esterase enzyme activity, and an indicator agent for detecting a phosphatase enzyme activity, wherein each of the plurality of indicator agents comprises a detectable reporter group. A method of using the culture device is also provided.
    Type: Grant
    Filed: February 25, 2013
    Date of Patent: December 30, 2014
    Assignee: 3M Innovative Properties Company
    Inventors: Sailaja Chandrapati, Tera M. Nordby
  • Patent number: 8911968
    Abstract: High-yield antibiotics producing strain, preparation method and use thereof are provided in the present invention. The high-yield strain is a mutagenized strain derived from Colephoma empetri, and deposited in CGMCC with the accession number of CGMCC 4129. The preparation method comprises the following steps: (a) mixing a seed liquid of Colephoma empetri of Accession No. FERM BP-2635 with nitrosoguanidine to obtain a mixture a; (b) mixing said mixture a with a wall-breaking enzyme to obtain protoplasts; (c) regenerating said protoplasts to obtain single colonies; and (d) culturing said single colonies to obtain said mutagenized strain. The obtained strain has stable genetic and producing property, produces little impurities in fermentation, and is suitable for industrialization.
    Type: Grant
    Filed: December 15, 2011
    Date of Patent: December 16, 2014
    Assignee: Shanghai Techwell Biopharmaceutical Co., Ltd.
    Inventors: Yi Chen, Shidong Liu, Zhaoli Zhang, Chunxia Wang, Jing Kang, Xiaoming Ji
  • Patent number: 8883993
    Abstract: The present invention provides novel promoter and terminator sequences for use in gene expression in eukaryotic cells, such as algal cells. The invention further provides expression cassettes comprising a promoter, as described herein, operably linked to a gene. The invention further provides expression vectors and host eukaryotic cells, such as algal cells, for expressing a protein encoded by the gene; and methods for stably transforming eukaryotic algae such as Tetraselmis with transgenes.
    Type: Grant
    Filed: December 4, 2012
    Date of Patent: November 11, 2014
    Assignee: ExxonMobil Research and Engineering Company
    Inventors: Jane C. Schneider, Soyan Lieberman, Bo Liu, Eric R. Moellering, John H. Verruto, Amanda Skaggs
  • Publication number: 20140329295
    Abstract: A process including: (a) mixing a microbial biomass, comprising oil-containing microbes, and at least one grinding agent capable of absorbing oil, to provide a disrupted biomass mix; (b) blending at least one binding agent with said disrupted biomass mix to provide a fixable mix capable of forming a solid pellet; and, (c) forming the solid pellet from the fixable mix. The process optionally includes extracting the solid pellet with a solvent to provide an extracted microbial oil.
    Type: Application
    Filed: July 18, 2014
    Publication date: November 6, 2014
    Inventors: MARIOS AVGOUSTI, TIMOTHY ALLAN BELL, OLIVER WALTER GUTSCHE, KEITH W. HUTCHENSON, ROBERT D. ORLANDI
  • Patent number: 8877481
    Abstract: The present invention relates to a method for enhancing growth of plants which comprises contacting a Trichoderma strain with the plant or a plant seed under conditions effective for the Trichoderma strain to colonize the roots of the plant or a plant grown from the plant seed, thereby creating a plant-Trichoderma system. The plant or plant seed is grown under conditions effective to sustain the plant-Trichoderma system in a planting medium and to enhance plant growth, where the Trichoderma strain is selected from the group consisting of Trichoderma atroviride strain WW10TC4 (ATCC accession number PTA 9707), Trichoderma harzianum strain RR17Bc (ATCC accession number PTA 9708), Trichoderma harzianum strain F11Bab (ATCC accession number PTA 9709), and combinations thereof.
    Type: Grant
    Filed: March 18, 2014
    Date of Patent: November 4, 2014
    Assignee: Cornell University
    Inventor: Gary E. Harman
  • Patent number: 8877480
    Abstract: The present invention relates to a method for enhancing growth of plants which comprises contacting a Trichoderma strain with the plant or a plant seed under conditions effective for the Trichoderma strain to colonize the roots of the plant or a plant grown from the plant seed, thereby creating a plant-Trichoderma system. The plant or plant seed is grown under conditions effective to sustain the plant-Trichoderma system in a planting medium and to enhance plant growth, where the Trichoderma strain is selected from the group consisting of Trichoderma atroviride strain WW10TC4 (ATCC accession number PTA 9707), Trichoderma harzianum strain RR17Bc (ATCC accession number PTA 9708), Trichoderma harzianum strain F11Bab (ATCC accession number PTA 9709), and combinations thereof.
    Type: Grant
    Filed: March 18, 2014
    Date of Patent: November 4, 2014
    Assignee: Cornell University
    Inventor: Gary E. Harman
  • Publication number: 20140323297
    Abstract: The present invention relates to a fertilizer comprising at least one microbe selected from the group consisting of Trichoderma viride (NRRL B-50520), Scopulariopsis brevicaulis (NRRL B-50521), Scopulariopsis brevicaulis (NRRL B-50522), and combinations thereof, and a substrate which is acted upon by the at least one microbe to release nitrogen. The invention also relates to a fertilizer comprising at least one microbe selected from a keratin degrading microorganism and a keratin substrate which is acted upon by the at least one microbe to release nitrogen. The invention further relates to a method for enhancing growth of plants with the fertilizer, and methods of making a fertilizer. The invention further relates to an isolated Trichoderma viride strain (NRRL B-50520) and two isolated Scopulariopsis brevicaulis strains (NRRL B-50521, NRRL B-50522).
    Type: Application
    Filed: November 21, 2012
    Publication date: October 30, 2014
    Inventors: Gary E. Harman, Xingen Lei
  • Patent number: 8871492
    Abstract: A fat-soluble fraction extracted from the fruiting body of Hericium erinaceum is demonstrated to inhibit the neuronal toxicity of amyloid beta-peptide (A?) and induce the synthesis of nerve growth factor (NGF), and has great potential as an active ingredient for pharmaceutical products, health food products, food products and/or beverages to prevent and/or treat dementia, especially Alzheimer-type dementia. This invention is to provide the bioactive fraction and its preparation method.
    Type: Grant
    Filed: May 2, 2008
    Date of Patent: October 28, 2014
    Inventors: Cun Zhuang, Hirokazu Kawagishi, Luyong Zhang, Hideo Anzai
  • Patent number: 8859228
    Abstract: The invention relates to a novel method for the production of ?-carotene from submerged cultures of mucoral fungi such as Blakeslea, Choanephora or Phycomyces by adding lectin to the culture medium and performing pH control once fermentation has started. The method involves ?-carotene recovery stage that makes it possible to simplify the process, optimize yields and increase purification of the product.
    Type: Grant
    Filed: July 18, 2001
    Date of Patent: October 14, 2014
    Assignee: DSM IP Assets B.V.
    Inventors: Javier Costa Perez, Antonio Estrella Castro, Ana Teresa Marcos Rodriguez, J. Emiliano Gonzalez De Prado, Enrique R. Peiro Cezon, Alfonso Collados De La Vieja, Manuel Esteban Morales
  • Publication number: 20140302560
    Abstract: A method of creating an extract of myceliated agricultural product for human consumption includes providing an agricultural substrate such as rice, where the agricultural substrate has been inoculated by liquid media comprising an aliquot of culture derived from liquid-state fermentation. The culture being selected from the group consisting of Basidiomycota and Ascomycota fungi. Next, the step of enabling mycelium growth on the substrate by controlling temperature, humidity and sterility of the environment. After mycelium growth on the substrate reaches a desired stage, then the step of boiling the substrate in water and separating the water-substrate mixture into aqueous component and non-aqueous components creates an extraction.
    Type: Application
    Filed: March 15, 2013
    Publication date: October 9, 2014
    Inventor: Brooks John Kelly
  • Publication number: 20140295543
    Abstract: The present invention concerns methods and compositions involving RNase III and polypeptides containing RNase III domains to generate RNA capable of triggering RNA-mediated interference (RNAi) in a cell. In some embodiments, the RNase III is from a prokaryote. RNase III activity will cleave a double-stranded RNA molecule into short RNA molecules that may trigger or mediate RNAi (siRNA). Compositions of the invention include kits that include an RNase III domain-containing polypeptide. The present invention further concerns methods using polypeptides with RNase III activity for generating RNA molecules that effect RNAi, including the generation of a number of RNA molecules to the same target.
    Type: Application
    Filed: December 16, 2013
    Publication date: October 2, 2014
    Applicant: APPLIED BIOSYSTEMS, LLC
    Inventors: Lance FORD, David BROWN
  • Patent number: 8846340
    Abstract: The present invention relates to non-catalytic carbohydrate-binding modules (CBM) belonging to a new family of CBM's. A CBM of the invention was found attached to a glycosyl hydrolase family 61 (GH61) polypeptide and was shown to have little homology with known CBM's indicating that it is the first known member of a new family of CBM's. The present invention further relates to CBM's preferably exhibiting binding affinity for cellulose; to a method of producing such CBM's; and to methods for using such CBM's in the textile, detergent and cellulose fiber processing industries, for purification of polypeptides, immobilization of active enzymes, baking, manufacturing of biofuel, modification of plant cell walls.
    Type: Grant
    Filed: October 26, 2004
    Date of Patent: September 30, 2014
    Assignee: Novozymes A/S
    Inventors: Kirk Matthew Schnorr, Lars Lehmann Hylling Christensen
  • Publication number: 20140287473
    Abstract: The present invention relates methods and compositions for producing an organic substance from fermenting microorganism using simultaneous saccharification and fermentation. One aspect of the invention provides producing alcohol by simultaneous saccharification and fermentation by combining a cellulosic substrate, and whole fermentation broth, and an ethanologenic microorganism under conditions conducive both to hydrolysis of cellulose to glucose and to conversion of glucose to alcohol.
    Type: Application
    Filed: March 22, 2013
    Publication date: September 25, 2014
    Applicant: DANISCO US INC.
    Inventors: Mian Li, Colin Mitchinson, Landon M. Steele
  • Patent number: 8841090
    Abstract: An L-arabinose utilizing yeast strain is provided for the production of ethanol by introducing and expressing bacterial araA, araB and araD genes. L-arabinose transporters are also introduced into the yeast to enhance the uptake of arabinose. The yeast carries additional genomic mutations enabling it to consume L-arabinose, even as the only carbon source, and to produce ethanol. A yeast strain engineered to metabolize arabinose through a novel pathway is also disclosed. Methods of producing ethanol include utilizing these modified yeast strains.
    Type: Grant
    Filed: January 17, 2013
    Date of Patent: September 23, 2014
    Assignee: Alliance for Sustainable Energy, LLC
    Inventors: Min Zhang, Arjun Singh, Pirkko Suominen, Eric Knoshaug, Mary Ann Franden, Eric Jarvis
  • Publication number: 20140273108
    Abstract: A method of producing hydrolysate from cellulosic material by adding hydrothermally treated stillage or a fraction thereof to cellulosic material, treating the mixture of hydrothermally treated stillage and cellulosic material with at least one hydrolyzing enzyme, and hydrolyzing and converting complex carbohydrates in the cellulosic material. The hydrolysate produced by the method. Ethanol, organic acids, and organism metabolites produced by the method. Biomass produced by the method. A method of increasing sugar production rate and yield of sugars from cellulosic material by adding hydrothermally treated stillage or a fraction thereof to cellulosic material, treating the mixture of hydrothermally treated stillage and cellulosic material with at least one hydrolyzing enzyme, and hydrolyzing complex carbohydrates in the cellulosic material and forming sugars.
    Type: Application
    Filed: March 17, 2014
    Publication date: September 18, 2014
    Applicant: Valicor, Inc
    Inventor: James Robert Bleyer
  • Patent number: 8835152
    Abstract: Compositions of peptides and surface-active agents are described, as are methods of making and using such compositions. The compositions are capable of affecting metabolic rates in biological systems, and to accelerate nutrient uptake without a concomitant increase in biofilm production.
    Type: Grant
    Filed: December 4, 2012
    Date of Patent: September 16, 2014
    Assignee: Advanced Biocatalytics Corporation
    Inventor: Carl W. Podella
  • Patent number: 8815580
    Abstract: The present application relates to the field of glyco-engineering, more specifically to eukaryotic cells wherein both an endoglucosaminidase and a glycoprotein are present. These cells can be used to deglycosylate or partly deglycosylate the (exogenous) glycoprotein, in particular without the need for adding an extra enzyme. Methods are also provided for the application of these cells in protein production. According to one specific aspect, the eukaryotic cells and methods are glyco-engineered yeast cells in which additionally at least one exogenous enzyme needed for complex glycosylation is present, e.g. allowing easier separation of differentially glycosylated glycoproteins.
    Type: Grant
    Filed: August 10, 2009
    Date of Patent: August 26, 2014
    Assignees: VIB VZW, Universiteit Gent
    Inventors: Nico L. M. Callewaert, Karen De Pourcq, Steven Geysens, Leander Meuris
  • Publication number: 20140235500
    Abstract: A system for the high-throughput screening of mechanical properties of cells and/or particles is provided. In certain embodiments the system comprises a first structure comprising a plurality of sample receiving wells; a second structure comprising a plurality of exit ports; a porous structure disposed between said plurality of sample receiving wells and said plurality of exit ports and in communication with said plurality of sample receiving wells and in communication with said plurality of exit ports, and said first structure, said second structure and said porous structure are sealed or configured such that when sufficient pressure is applied to sample receiving wells in said first structure said cells or particles migrate through the porous structure into said exit ports, and wherein the mean or the median pore size of said porous structure is smaller than the mean or median cross-section of a cell or particle that is to be screened.
    Type: Application
    Filed: October 15, 2012
    Publication date: August 21, 2014
    Applicant: The Regents of the University of California
    Inventors: Amy Rowat, Dongping Qi
  • Publication number: 20140227767
    Abstract: The invention provide isolated peptides, protides and conjugates having novel peptide sequences which are able to induce antimicrobial, anti-cancer, anti-inflammatory, anti-proliferative or programmed cell death activity. The invention also provides a method of inducing programmed cell death in a cell by contacting the cell with an isolated peptide, protide or conjugate described herein. In some aspects, the method can be used in the diagnosis, prevention, or treatment of a disease, such as an infection, cancer, autoimmune disease, or inflammatory disease.
    Type: Application
    Filed: May 1, 2013
    Publication date: August 14, 2014
    Inventors: Michael R. YEAMAN, Nannette Y. YOUNT, Eric P. BRASS
  • Publication number: 20140220663
    Abstract: The present invention provides a method for reducing the viscosity of a microorganism-containing suspension or concentrate and a method for obtaining a microorganism concentrate, wherein a monosaccharide and/or disaccharide and/or sugar alcohol is added to said microorganism-containing suspension or concentrate.
    Type: Application
    Filed: July 6, 2012
    Publication date: August 7, 2014
    Applicant: DUPONT NUTRITION BIOSCIENCES APS
    Inventors: Michel Cadenel, Patrick Chaigneau, Guy D'angloz, Erwan Henri, Jean Pierre Rocher