Fusarium Patents (Class 435/254.7)
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Patent number: 8507224Abstract: Lower eukaryote host cells in which an endogenous or heterologous Ca2+ ATPase is overexpressed are described. Also described are lower eukaryote host cells in which a calreticulin and/or ERp57 protein are overexpressed. These host cells are useful for producing recombinant glycoproteins that have reduced O-glycosylation.Type: GrantFiled: August 10, 2009Date of Patent: August 13, 2013Assignee: GlycoFi, Inc.Inventor: Byung-Kwon Choi
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Publication number: 20130203126Abstract: The present invention relates to alpha-amylases, nucleic acids encoding the alpha-amylases, methods of producing the alpha-amylases, and methods of using the alpha-amylases.Type: ApplicationFiled: April 5, 2013Publication date: August 8, 2013Applicant: NOVOZYMES A/SInventor: NOVOZYMES A/S
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Publication number: 20130203128Abstract: In alternative embodiments, the invention provides polypeptides having a lignocellulolytic (lignocellulosic) activity, e.g., a ligninolytic and cellulolytic activity, including, e.g., a glycosyl hydrolase, a cellulase, an endoglucanase, a cellobiohydrolase (cbhl) (e.g., an exo-cellobiohydrolase, e.g., having an “exo” activity that can processively release cellobiose units ?-1,4 glucose-glucose disaccharide), a beta-glucosidase, a xylanase, a mannanse, a xylosidase (e.g., a (?-xylosidase) and/or an arabinofuranosidase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In one embodiment, the invention provides thermostable and thermotolerant forms of polypeptides of the invention. The polypeptides and nucleic acids of the invention are used in a variety of pharmaceutical, agricultural and industrial contexts; for example, as enzymes for the bioconversion of a biomass, e.g.Type: ApplicationFiled: October 6, 2011Publication date: August 8, 2013Applicant: BP Corporation North America Inc,Inventors: Justin T. Stege, Alexander Varvak, John Poland, Chris S. Lyon, Shaun Healey, Peter Luginbuhl
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Publication number: 20130196387Abstract: The present invention provides isolated polypeptides having endoglucanase activity and isolated polynucleotides encoding the polypeptides. The invention also provides nucleic acid constructs, vectors, and host cell comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: November 11, 2011Publication date: August 1, 2013Applicant: Novozymes Inc.Inventors: Ye Liu, Junxin Duan, Lan Tang
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Publication number: 20130198910Abstract: The present invention relates to isolated polypeptides having cellobiohydrolase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: August 30, 2011Publication date: August 1, 2013Applicants: NOVOYZMES A/S, NOVOZYMES, INC.Inventor: Nikolaj Spodsberg
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Publication number: 20130189733Abstract: The present invention relates to a method to improve the secretion of a protein of interest by a filamentous fungal cell comprising inducing a phenotype in the cell selected from the group consisting of a lowered ERAD, an elevated UPR that does not induce an elevated ERAD, wherein ERAD preferably is lowered. The invention further relates to the filamentous fungal cell comprising the phenotype described above. The invention also relates to polynucleotides and polypeptides whose expression can be modulated in the filamentous fungal cell to obtain the above-described phenotype.Type: ApplicationFiled: January 25, 2013Publication date: July 25, 2013Applicant: DSM IP ASSETS B.V.Inventors: CORNELIS MARIA JACOBUS SAGT, CORNELIS THEODORUS VERRIPS, WALRAVEN HENRY MULLER, NOEL NICOLAAS MARIA ELISABETH VAN PEIJ
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Publication number: 20130177947Abstract: The present disclosure is generally directed to glycosyl hydrolase enzymes, compositions comprising such enzymes, and methods of using the enzymes and compositions, for example for the saccharification of cellulosic and hemicellulosic materials into sugars.Type: ApplicationFiled: September 22, 2010Publication date: July 11, 2013Applicant: DANISCO US INC.Inventors: Benjamin Bower, Megan Yee Hsi, Thijs Kaper, Bradley R. Keleman, Suzanne E. Lantz, Edmund A. Larenas, Colin Mitchinson, Steven Kim, William D. Hitz, Mark Emptage, Keith Dumont Wing
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Publication number: 20130180013Abstract: The present invention relates to isolated polypeptides having xylanase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: August 30, 2011Publication date: July 11, 2013Applicants: NOVOYZMES A/S, NOVOZYMES, INC.Inventor: Nikolaj Spodsberg
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Publication number: 20130177964Abstract: Compositions and methods relating to an alpha-amylase enzyme obtained from Nesterenkonia and related spp. are described.Type: ApplicationFiled: January 20, 2011Publication date: July 11, 2013Applicant: DANISCO US INC.Inventors: Brian E. Jones, Marc Kolkman
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Patent number: 8481320Abstract: The present invention relates to methods for increasing homologous recombination of a nucleic acid sequence introduced into a host cell, comprising: (a) introducing into a population of filamentous fungal host cells a first nucleic acid sequence encoding a recombination protein and a second nucleic acid sequence comprising one or more regions which are homologous with the genome of the filamentous fungal host cell, wherein (i) the recombination protein promotes the recombination of the one or more regions with the corresponding homologous region in the host's genome to incorporate the second nucleic acid sequence by homologous recombination, and (ii) the number of host cells comprising the incorporated second nucleic acid sequence in the population is increased at least 20% compared to the same population without the first nucleic acid sequence; (b) and isolating from the population a filamentous fungal cell comprising the incorporated second nucleic acid sequence.Type: GrantFiled: December 10, 2012Date of Patent: July 9, 2013Assignee: Novozymes, Inc.Inventors: Paul Harris, Howard Brody
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Publication number: 20130171718Abstract: The present disclosure relates to ?-class carbonic anhydrase polypeptides having improved properties including increased thermostability and/or stability in the presence of amine compounds, ammonia, or carbonate ion. The present disclosure also provides formulations and uses of the polypeptides for accelerating the absorption of carbon dioxide from a gas stream into a solution as well as for the release of the absorbed carbon dioxide for further treatment and/or sequestering. Also provided are polynucleotides encoding the carbonic anhydrase polypeptides and host cells capable of expressing them.Type: ApplicationFiled: December 20, 2012Publication date: July 4, 2013Applicant: CODEXIS, INC.Inventor: Codexis, Inc.
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Publication number: 20130164263Abstract: A fungus, for biological control, having Fusarium graminearum virus 1-DK21 transmitted thereto and a method for biological control using the same and discloses a fungus, for biological control, having an infectious Fusarium virus transmitted thereto, a composition for biological control comprising the same, and a method for biological control using the same. It also provides a method for biological control, which includes bringing a virulent wild-type species into contact with a fungus having a virus inserted therein. It also provides a method of transmitting an infectious Fusarium virus to a vegetatively incompatible strain by a protoplast fusion.Type: ApplicationFiled: December 21, 2012Publication date: June 27, 2013Applicant: SNU R&DB FOUNDATIONInventor: SNU R&DB FOUNDATION
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Publication number: 20130149742Abstract: The present disclosure relates to filamentous fungal host strains and recombinant DNA constructs for creation and use thereof. The filamentous fungal host strains are particularly useful for achieving reliable expression of recombinant enzymes and variants.Type: ApplicationFiled: June 3, 2011Publication date: June 13, 2013Applicant: Danisco US Inc.Inventors: Benjamin S. Bower, Thijs Kaper, Bradley R. Kelemen
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Publication number: 20130152234Abstract: The present invention relates to isolated polypeptides having lipase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.Type: ApplicationFiled: February 7, 2013Publication date: June 13, 2013Applicants: NOVOZYMES A/S, NOVOZYMES, INC.Inventors: Novozymes, Inc., Novozymes A/S
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Publication number: 20130143291Abstract: The present invention relates to methods for producing monoterpenes, particularly tricyclene, by culturing microbial organisms that express a terpene synthase and optionally a prenyl transferase.Type: ApplicationFiled: March 30, 2011Publication date: June 6, 2013Applicant: Codexis, Inc.Inventors: Robert McDaniel, Galit Meshulam-Simon, Oscar Alvizo, Xiyun Zhang
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Publication number: 20130145501Abstract: The invention relates to a polypeptide having carbohydrate material degrading activity which comprises the amino acid sequence set out in SEQ ID NO: 2 or an amino acid sequence encoded by the nucleotide sequence of SEQ ID NO: 1 or SEQ ID NO: 4, or a variant polypeptide or variant polynucleotide thereof, wherein the variant polypeptide has at least 96% sequence identity with the sequence set out in SEQ ID NO: 2 or the variant polynucleotide encodes a polypeptide that has at least 96% sequence identity with the sequence set out in SEQ ID NO: 2. The invention features the full length coding sequence of the novel gene as well as the amino acid sequence of the full-length functional protein and functional equivalents of the gene or the amino acid sequence. The invention also relates to methods for using the polypeptide in industrial processes. Also included in the invention are cells transformed with a polynucleotide according to the invention suitable for producing these proteins.Type: ApplicationFiled: April 23, 2010Publication date: June 6, 2013Applicant: DSM IP ASSETS B.V.Inventors: Cornelis Maria Jacobus Sagt, Margot Elisabeth Francoise Schooneveld-Bergmans, Johannes Andries Roubos, Alrik Pieter Los
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Publication number: 20130143301Abstract: The present disclosure is generally directed to enzymes and in particular beta-glucosidase variants. Also described are nucleic acids encoding beta-glucosidase variants, compositions comprising beta-glucosidase variants, methods of using beta-glucosidase variants, and methods of identifying additional useful beta-glucosidase variants.Type: ApplicationFiled: November 19, 2010Publication date: June 6, 2013Applicant: Danisco US Inc.Inventors: Richard R. Bott, Thijs Kaper, Bradley Kelemen, Frits Goedegebuur, Ronaldus Hommes, Slavko Kralj, Paulien Kruithof, Igor Nikolaev
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Publication number: 20130137149Abstract: The invention is directed to a genetically modified microorganism for the extracellular production of free fatty acids and esters thereof, wherein said microorganism is characterised by a modified lipid biosynthesis metabolic pathway: for example reduced fatty acyl-coA synthetase activity that enables the microorganism to over-produced and secrete of esters of fatty acids (Biodiesel) into the surrounding medium, using one or more of: glucose, starch, lignocellulose and a glycerol-based substrate, as a carbon source. The invention further provides a method for the extracellular production of free fatty acids and esters thereof, comprising the use of said genetically modified organism, and a growth medium adapted for said method.Type: ApplicationFiled: June 20, 2011Publication date: May 30, 2013Inventors: Ambareesh Govind Phadnavis, Peter Ruhdal Jensen
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Publication number: 20130137152Abstract: The present invention relates to isolated polypeptides having glucoamylase activity, catalytic domains, carbohydrate binding domains and polynucleotides encoding the polypeptides, catalytic domains or carbohydrate binding domains. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains or carbohydrate binding domains.Type: ApplicationFiled: November 28, 2012Publication date: May 30, 2013Applicants: Novozymes North America, Inc., Novozymes A/SInventors: Novozymes A/S, Novozymes North America, Inc.
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Publication number: 20130130326Abstract: The present invention relates to isolated polypeptides having beta-glucosidase activity, beta-xylosidase activity, or beta-glucosidase and beta-xylosidase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: November 16, 2012Publication date: May 23, 2013Applicants: NOVOZYMES, INC., NOVOZYMES A/SInventors: Novozymes A/S, Novozymes, Inc.
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Publication number: 20130130327Abstract: The present invention relates to isolated polypeptides having beta-glucosidase activity, beta-xylosidase activity, or beta-glucosidase and beta-xylosidase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: November 16, 2012Publication date: May 23, 2013Applicants: NOVOZYMES, INC., NOVOZYMES A/SInventors: Novozymes A/S, Novozymes, Inc.
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Publication number: 20130130325Abstract: The present invention relates to isolated polypeptides having beta-glucosidase activity, beta-xylosidase activity, or beta-glucosidase and beta-xylosidase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: November 16, 2012Publication date: May 23, 2013Applicants: NOVOZYMES, INC., NOVOZYMES A/SInventors: Novozymes A/S, Novozymes, Inc.
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Publication number: 20130130248Abstract: The present disclosure provides variant Csy4 endoribonucleases, nucleic acids encoding the variant Csy4 endoribonucleases, and host cells genetically modified with the nucleic acids. The variant Csy4 endoribonucleases find use in a variety of applications, which are also provided. The present disclosure also provides methods of detecting a specific sequence in a target polyribonucleotide; and methods of regulating production of a target RNA in a eukaryotic cell.Type: ApplicationFiled: November 7, 2012Publication date: May 23, 2013Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIAInventor: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
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Publication number: 20130122149Abstract: The present invention relates to isolated polypeptides having glucose dehydrogenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: January 16, 2013Publication date: May 16, 2013Applicant: NOVOZYMES A/SInventor: NOVOZYMES A/S
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Publication number: 20130123163Abstract: The present invention relates to isolated polypeptides having lipase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.Type: ApplicationFiled: January 22, 2013Publication date: May 16, 2013Applicants: NOVOZYMES A/S, NOVOZYMES, INC.Inventors: Novozymes, Inc., Novozymes A/S
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Publication number: 20130122545Abstract: The present invention relates to isolated polypeptides having protease activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.Type: ApplicationFiled: January 17, 2013Publication date: May 16, 2013Applicant: NOVOZYMES A/SInventor: NOVOZYMES A/S
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Publication number: 20130115662Abstract: The invention relates to polypeptides having peroxygenase activity and compositions comprising such polypeptides, their encoding polynucleotides, expression vectors and recombinant host cells comprising such polynucleotides or vectors, methods of producing the polypeptides, as well as methods of application and uses thereof, including a process for enzymatic, regioselective oxygenation of N-heterocycles of the general formula (I) to the corresponding N-oxides of the formula (II), by converting N-heterocycles of the formula (I) with a peroxidase polypeptide in the presence of at least one oxidizing agent in a one-stage reaction process.Type: ApplicationFiled: December 20, 2012Publication date: May 9, 2013Applicant: NOVOZYMES A/SInventor: Novozymes A/S
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Publication number: 20130109055Abstract: The present disclosure is directed to the use of mammalian promoters to drive recombinant expression in filamentous fungal cells. In certain aspects, the present disclosure provides an expression cassette useful for the expression of polypeptide in filamentous fungal cells. Also provided herein, are vectors and recombinant filamentous fungal cells comprising the expression cassettes of the present disclosure, and methods of making and using the same for recombinant polypeptide expression.Type: ApplicationFiled: October 31, 2012Publication date: May 2, 2013Applicant: BP Corporation North America Inc.Inventor: BP Corporation North America Inc.
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Publication number: 20130109054Abstract: The present disclosure is directed to the use of plant promoters to drive recombinant expression in filamentous fungal cells. In certain aspects, the present disclosure provides an expression cassette useful for the expression of polypeptide in filamentous fungal cells. Also provided herein, are vectors and recombinant filamentous fungal cells comprising the expression cassettes of the present disclosure, and methods of making and using the same for recombinant polypeptide expression.Type: ApplicationFiled: October 31, 2012Publication date: May 2, 2013Applicant: BP CORPORATION NORTH AMERICA INC.Inventor: BP CORPORATION NORTH AMERICA INC.
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Publication number: 20130102042Abstract: The invention refers to fungal cells, and especially to oleaginous fungal cells that have been genetically modified to produce enzymes of the pyruvate dehydrogenase bypass route to enhance their lipid production. Especially the cells are modified to over-express genes encoding pyruvate decarboxylase (PDC), acetaldehyde dehydrogenase (ALD) and/or acetyl-CoA synthetase (ACS), optionally together with a gene encoding diacylglycerol acyltransferase (DAT), or to express genes encoding PDC together with ALD and/or ACS. Methods of producing lipids, biofuels and lubricants using the modified fungi are also disclosed as well as expression cassettes useful therein. A new enzyme having phosholipid: diacylglycerol acyltransferase (PDAT)activity it and a polynucleotide encoding it are also disclosed, which are useful in the lipid production. A recombinant Cryptococcus cell and its construction is described.Type: ApplicationFiled: June 21, 2011Publication date: April 25, 2013Applicant: TEKNOLOGIAN TUTKIMUSKESKUS VTTInventors: Kari Koivuranta, Laura Ruohonen, Merja Penttilä
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Publication number: 20130102035Abstract: The present invention relates to combinatorial variants of a parent glucoamylase that have altered properties for reducing the synthesis of condensation products during hydrolysis of starch. Accordingly the variants of a parent glucoamylase are suitable such as for use within brewing and glucose syrup production. Also disclosed are DNA constructs encoding the variants and methods of producing the glucoamylase variants in host cells.Type: ApplicationFiled: June 30, 2011Publication date: April 25, 2013Applicant: DUPONT NUTRITION BIOSCIENCES APSInventors: Peter Edvard Degn, Richard R. Bott, Casper Willem Vroemen, Martijn Silvan Scheffers, Wolfgang Aehle, Elin Petersen
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Publication number: 20130104264Abstract: The invention relates to a polypeptide comprising the amino acid sequence set out in SEQ ID NO: 2 or an amino acid sequence encoded by the nucleotide sequence of SEQ ID NO: 1, or a variant polypeptide or variant polynucleotide thereof, wherein the variant polypeptide has at least 96% sequence identity with the sequence set out in SEQ ID NO: 2 or the variant polynucleotide encodes a polypeptide that has at least 96% sequence identity with the sequence set out in SEQ ID NO: 2. The invention features the full length coding sequence of the novel gene as well as the amino acid sequence of the full-length functional polypeptide and functional equivalents of the gene or the amino acid sequence. The invention also relates to methods for using the polypeptide in industrial processes. Also included in the invention are cells transformed with a polynucleotide according to the invention suitable for producing these proteins.Type: ApplicationFiled: June 23, 2011Publication date: April 25, 2013Applicant: DSM IP ASSETS B.V.Inventors: Margot Elisabeth Francoise Schoonneveld-Bergmans, Wilbert Herman Marie Heijne, Rene Marcel De Jong, Robbertus Antonius Damveld
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Publication number: 20130095525Abstract: The present invention relates to isolated polynucleotides having leader sequence function. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods using the polynucleotides for production of polypeptides.Type: ApplicationFiled: June 23, 2011Publication date: April 18, 2013Applicant: NOVOZYMES A/SInventors: Hiroaki Udagawa, Rikako Taira, Shinobu Takagi
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Publication number: 20130095553Abstract: The invention relates to a polypeptide which comprises the amino acid sequence set out in SEQ ID NO: 2 or an amino acid sequence encoded by the nucleotide sequence of SEQ ID NO: 1, or a variant polypeptide or variant polynucleotide thereof, wherein the variant polypeptide has at least 76% sequence identity with the sequence set out in SEQ ID NO: 2 or the variant polynucleotide encodes a polypeptide that has at least 76% sequence identity with the sequence set out in SEQ ID NO: 2. The invention features the full length coding sequence of the novel gene as well as the amino acid sequence of the full-length functional polypeptide and functional equivalents of the gene or the amino acid sequence. The invention also relates to methods for using the polypeptide in industrial processes. Also included in the invention are cells transformed with a polynucleotide according to the invention suitable for producing these proteins.Type: ApplicationFiled: June 23, 2011Publication date: April 18, 2013Applicant: DSM IP ASSETS B.V.Inventors: Margot Elisabeth Francoise Schooneveld-Bergmans, Wilbert Herman Marie Heijne, Alrik Pieter Los
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Publication number: 20130095532Abstract: The invention relates to a polypeptide comprising the amino acid sequence set out in SEQ ID NO: 2 or an amino acid sequence encoded by the nucleotide sequence of SEQ ID NO: 1, or a variant polypeptide or variant polynucleotide thereof, wherein the variant polypeptide has at least 82% sequence identity with the sequence set out in SEQ ID NO: 2 or the variant polynucleotide encodes a polypeptide that has at least 82% sequence identity with the sequence set out in SEQ ID NO: 2. The invention features the full length coding sequence of the novel gene as well as the amino acid sequence of the full-length functional polypeptide and functional equivalents of the gene or the amino acid sequence. The invention also relates to methods for using the polypeptide in industrial processes. Also included in the invention are cells transformed with a polynucleotide according to the invention suitable for producing these proteins.Type: ApplicationFiled: June 23, 2011Publication date: April 18, 2013Applicant: DSM IP ASSETS B.V.Inventors: Margot Elisabeth Francoise Schoonneveld-Bergmans, Wilbert Herman Marie Heijne, Alrik Pieter Los
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Publication number: 20130095550Abstract: The present invention relates to isolated polynucleotides having promoter activity the use of the isolated polynucleotides for the production of a polypeptide. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing a desired polypeptide using the polypeptide having promoter activity.Type: ApplicationFiled: June 23, 2011Publication date: April 18, 2013Applicant: NOVOZYMES A/SInventors: Hiroshi Teramoto, Hiroaki Udagawa, Rikako Taira
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Publication number: 20130095554Abstract: The present invention relates to a modified Family 5 cellulase comprising a substitution of an amino acid at position 363 with a non-native alanine, serine or threonine, said position determined from alignment of the modified Family 5 cellulase with amino acids 71-397 of a Trichoderma reesei Cel5A amino acid sequence as set forth in SEQ ID NO:1 and enzyme mixtures comprising same. Additionally provided is a genetic construct comprising a nucleic acid sequence encoding the modified Family 5 cellulase and a genetically modified microbe comprising the genetic construct. The invention also provides a process for producing the modified Family 5 cellulase.Type: ApplicationFiled: March 11, 2011Publication date: April 18, 2013Applicant: Iogen Bio-Products CorporationInventors: Nabil Masri, Patrick St-Pierre, Sandra Mortimer, Christopher M.D. Hill
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Publication number: 20130095531Abstract: The invention relates to a polypeptide comprising the amino acid sequence set out in SEQ ID NO: 2 or an amino acid sequence encoded by the nucleotide sequence of SEQ ID NO: 1, or a variant polypeptide or variant polynucleotide thereof, wherein the variant polypeptide has at least 70% sequence identity with the sequence set out in SEQ ID NO: 2 or the variant polynucleotide encodes a polypeptide that has at least 70% sequence identity with the sequence set out in SEQ ID NO: 2. The invention features the full length coding sequence of the novel gene as well as the amino acid sequence of the full-length functional polypeptide and functional equivalents of the gene or the amino acid sequence. The invention also relates to methods for using the polypeptide in industrial processes. Also included in the invention are cells transformed with a polynucleotide according to the invention suitable for producing these proteins.Type: ApplicationFiled: June 23, 2011Publication date: April 18, 2013Applicant: DSM IP ASSETS B.V.Inventors: Margot Elisabeth Francoise Schooneveld-Bergmans, Wilbert Herman Marie Heijne, Rene Marcel De Jong, Robbertus Antonius Damveld
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Publication number: 20130090463Abstract: The present invention relates to isolated polynucleotides having promoter activity the juse of the isolated polynucleotides for the procuction of a polypeptide. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing a desired polypeptide using the polypeptide having promoter activity.Type: ApplicationFiled: June 23, 2011Publication date: April 11, 2013Applicant: NOVOZYMES A/SInventors: Hiroshi Teramoto, Hiroaki Udagawa
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Publication number: 20130089902Abstract: The present invention relates to methods of producing C4 dicarboxylic acids, such as malic acid, comprising: (a) cultivating a host cell comprising a polynucleotide encoding a C4 dicarboxylic acid transporter; and (b) recovering the C4 dicarboxylic acid. The present invention also relates to methods for increasing C4 dicarboxylic acid production, as well as host cells comprising the polynucleotides.Type: ApplicationFiled: June 2, 2011Publication date: April 11, 2013Applicant: Novozymes, Inc.Inventors: Sarah McFarland, Amanda Fischer
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Publication number: 20130089897Abstract: The present invention relates to isolated polypeptides having isoamylase activity derived from Dyella japonica and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. The invention also relates to the use of said polypeptide having isoamylase activity for producing glucose syrup, fructose syrup, maltose syrup or maltitol.Type: ApplicationFiled: December 10, 2012Publication date: April 11, 2013Applicant: NOVOZYMES A/SInventor: NOVOZYMES A/S
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Publication number: 20130084604Abstract: The present invention relates to a method for genetically modifying a filamentous fungus host for improved protein production. The method comprises that a filamentous fungus host is genetically modified to overexpress or to be deficient of specific genes. The invention relates also to the modified hosts. Furthermore, the invention relates to a method for improved production or for producing an improved composition of proteins, such as cellulases, hemicellulases, other proteins involved in the degradation of lignocellulosic material, or other proteins, in a filamentous fungus host.Type: ApplicationFiled: May 30, 2011Publication date: April 4, 2013Applicant: TEKNOLOGIAN TUTKIMUSKESKUS VTTInventors: Tiina Pakula, Markku Saloheimo, Mari Hakkinen, Ann Westerholm-Parvinen, Merja Penttila, Marika Vitikainen
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Publication number: 20130084617Abstract: The present invention relates to host cells transformed with a nucleic acid sequence encoding a eukaryotic xylose isomerase obtainable from an anaerobic fungus. When expressed, the sequence encoding the xylose isomerase confers to the host cell the ability to convert xylose to xylulose which may be further metabolized by the host cell. Thus, the host cell is capable of growth on xylose as carbon source. The host cell preferably is a eukaryotic microorganism such as a yeast or a filamentous fungus. The invention further relates to processes for the production of fermentation products such as ethanol, in which a host cell of the invention uses xylose for growth and for the production of the fermentation product. The invention further relates to nucleic acid sequences encoding eukaryotic xylose isomerases and xylulose kinases as obtainable from anaerobic fungi.Type: ApplicationFiled: November 29, 2012Publication date: April 4, 2013Applicant: C5 Yeast Company B.VInventor: C5 Yeast Company B.V
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Publication number: 20130078674Abstract: The present invention relates to a method for genetically modifying a filamentous fungus host for improved protein production. The method comprises that a filamentous fungus host is genetically modified to overexpress or to be deficient of specific genes. The invention relates also to the modified hosts. Furthermore, the invention relates to a method for improved production or for producing an improved composition of proteins, such as cellulases, hemicellulases, other proteins involved in the degradation of lignocellulosic material, or other proteins, in a filamentous fungus host.Type: ApplicationFiled: May 30, 2011Publication date: March 28, 2013Applicant: TEKNOLOGIAN TUTKIMUSKESKUS VTTInventors: Tiina Pakula, Markku Saloheimo, Mari Hakkinen, Ann Westerholm-Parvinen, Merja Penttila, Marika Vitikainen
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Publication number: 20130071908Abstract: The invention features methods for producing isoprene from cultured cells. The invention also provides compositions that include these cultured cells.Type: ApplicationFiled: August 30, 2012Publication date: March 21, 2013Applicants: The Goodyear Tire & Rubber Company, Danisco US Inc.Inventors: Marguerite A. CERVIN, Gregory M. Whited, Gopal K. Chotani, Fernando Valle, Karl J. Sanford, Joseph C. McAuliffe, Frank J. Feher, Aaron S. Puhala, Andrei Miasnikov, Richard La Duca, Caroline M. Peres
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Publication number: 20130061354Abstract: The invention relates to a polypeptide comprising the amino acid sequence set out in SEQ ID NO: 2 or an amino acid sequence encoded by the nucleotide sequence of SEQ ID NO: 1, or a variant polypeptide or variant polynucleotide thereof, wherein the variant polypeptide has at least 93% sequence identity with the sequence set out in SEQ ID NO: 2 or the variant polynucleotide encodes a polypeptide that has at least 93% sequence identity with the sequence set out in SEQ ID NO: 2. The invention features the full length coding sequence of the novel gene as well as the amino acid sequence of the full-length functional polypeptide and functional equivalents of the gene or the amino acid sequence. The invention also relates to methods for using the polypeptide in industrial processes. Also included in the invention are cells transformed with a polynucleotide according to the invention suitable for producing these proteins.Type: ApplicationFiled: February 11, 2011Publication date: March 7, 2013Applicant: DSM IP ASSETSInventors: Cornelis Maria Jacobus Sagt, Margot Elisabeth Francoise Schoonneveld-Bergmans, Johannes Andries Roubos, Alrik Pieter Los
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Publication number: 20130059353Abstract: Provided herein are host cells comprising carbonic anhydrase activity, wherein the cells are capable of producing C4-dicarboxylic acid. Also provided are methods of producing C4-dicarboxylic acid comprising (a) cultivating the host cells having carbonic anhydrase activity in a medium under suitable conditions to produce C4-dicarboxylic acid; and (b) recovering the C4-dicarboxylic acid.Type: ApplicationFiled: August 17, 2012Publication date: March 7, 2013Applicant: NOVOZYMES, INC.Inventors: Sarah McFarland, Stephen Brown, Sheryl Luttringer
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Host Cells and Methods for Producing 1-Deoxyxylulose 5-phosphate (DXP) and/or a DXP Derived Compound
Publication number: 20130052692Abstract: The present invention provides for a genetically modified host cell capable of producing 1-deoxyxylulose 5-phosphate or 1-deoxy-D-xylulose 5-phosphate (DXP) (12), and optionally one or more DXP derived compounds, comprising: (a) a mutant RibB, or functional variant thereof, capable of catalyzing xylulose 5-phosphate and/or ribulose 5-phosphate to DXP, or (b) a YajO, or functional variant thereof, and a XylB, or functional variant thereof.Type: ApplicationFiled: August 16, 2012Publication date: February 28, 2013Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIAInventors: James Kirby, Jeffrey L. Fortman, Minobu Nishimoto, Jay D. Keasling -
Publication number: 20130052679Abstract: A method for analyzing L-threonine contained in an specimen, which includes the steps of mixing a sample containing the specimen with an L-threonine dehydrogenase derived from Cupriavidus necator and a coenzyme NAD+ and analyzing the amount of NADH or 2-amino-3-oxobutyric acid after a predetermined period; an L-threonine dehydrogenase derived from Cupriavidus necator, which is a novel L-threonine dehydrogenase (TDH; EC 1.1.1.103) and can be utilized in the above-mentioned analysis method; a method for preparing a gene or the like to be used in the preparation of the enzyme, or a method for preparing the enzyme; an L-threonine analysis kit which includes (A) the L-threonine dehydrogenase and (B) a coenzyme NAD+; an enzyme preparation for use in the analysis of L-threonine, which includes the L-threonine dehydrogenase contained in a buffer solution; and an enzyme sensor utilizing the L-threonine dehydrogenase.Type: ApplicationFiled: September 4, 2012Publication date: February 28, 2013Inventors: Yasuhisa Asano, Techawaree Ueatrongchit
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Publication number: 20130052686Abstract: The present invention relates to isolated polypeptides having alpha-L-arabinofuranosidase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: October 26, 2012Publication date: February 28, 2013Applicant: NOVOZYMES A/SInventors: Michelle Maranta, Kimberly Brown, James Langston