Mucor Patents (Class 435/254.8)
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Publication number: 20120023626Abstract: The present invention relates to isolated polypeptides having endoglucanase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.Type: ApplicationFiled: October 5, 2011Publication date: January 26, 2012Applicants: NOVOZYMES, INC., NOVOZYMES A/SInventors: Paul Harris, Elena Vlasenko, Elizabeth Zaretsky, Marcus Sakari Kauppinnen, Sarah Teter, Kimberly Brown
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Publication number: 20120015408Abstract: The invention provides variants of the Thermoanaerobacter brockii CglT beta-glucosidase that have improve beta-glucosidase activity compared to the wild type enzyme. The invention also provides polynucleotides that encode the variants, as well as methods of producing the variants, enzyme compositions comprising the variants, and methods for using the variants in industrial applications.Type: ApplicationFiled: February 26, 2010Publication date: January 19, 2012Applicant: CODEXIS, INC.Inventors: Dipnath Baidyaroy, Louis Clark, Lisa M. Newman, Charlene Ching
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Publication number: 20120009300Abstract: The present invention relates to use of an anti-staling GH-61 polypeptide for preparing an edible product.Type: ApplicationFiled: September 23, 2011Publication date: January 12, 2012Applicant: Novozymes A/SInventors: Kirk Matthew Schnorr, Sara Landvik, Tina Spendler, Lars Lehmann Hylling Christensen
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Publication number: 20120011619Abstract: The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: September 21, 2011Publication date: January 12, 2012Applicant: NOVOZYMES, INC.Inventors: Alfredo Lopez de Leon, Hanshu Ding, Kimberly Brown
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Publication number: 20120003703Abstract: The invention relates to recombinant expression of variant forms of C1 CBH1a and homologs thereof, having improved thermostability, low-pH tolerance, specific activity and other desirable properties. Also provided are methods for producing ethanol and other valuable organic compounds by combining cellobiohydrolase variants with cellulosic materials.Type: ApplicationFiled: May 13, 2011Publication date: January 5, 2012Applicant: Codexis, Inc.Inventors: Vesna Mitchell, Grzegorz Wojciechowski, Oscar Alvizo
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Publication number: 20110311693Abstract: The present invention relates to isolated polypeptides having tyrosinase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: March 16, 2010Publication date: December 22, 2011Applicants: Chr. Hansen A/S, Novozymes A/SInventors: Kirk Matthew Schnorr, Jeppe Wegener Tams
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Publication number: 20110312046Abstract: The present invention relates to methods of producing C4-dicarboxylic acids, such as malic acid, comprising: (a) cultivating a host cell comprising a polynucleotide encoding a C4-dicarboxylic acid transporter; and (b) recovering the C4-dicarboxylic acid. The present invention also relates to methods for increasing C4-dicarboxylic acid production, as well as host cells comprising the polynucleotides.Type: ApplicationFiled: June 21, 2011Publication date: December 22, 2011Applicant: NOVOZYMES, INC.Inventors: SHERYL LUTTRINGER, DEBBIE YAVER
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Publication number: 20110312047Abstract: The present invention relates to isolated polypeptides having C4-dicarboxylic acid transporter activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, and methods of producing C4-dicarboxylic acids, such as malic acid.Type: ApplicationFiled: June 21, 2011Publication date: December 22, 2011Applicant: Novozymes, Inc.Inventors: Amanda Fischer, Debbie Yaver
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Publication number: 20110306750Abstract: The present invention relates to variants of a parent antimicrobial peptide. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.Type: ApplicationFiled: June 2, 2011Publication date: December 15, 2011Applicant: Novozymes A/SInventors: Hans-Henrik Kristensen Hoegenhaug, Per Holse Mygind, Thomas Kruse, Dorotea Raventos Segura, Dorthe Hoj Sandvang, Soren Neve
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Publication number: 20110296557Abstract: The present invention relates to isolated polypeptides having lipase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: December 9, 2009Publication date: December 1, 2011Applicant: Novozymes, Inc.Inventor: Michael Rey
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Publication number: 20110294191Abstract: Described are compositions and methods relating to filamentous fungal cells genetically engineered to provide increased production of aspartic proteases, such as PEPAa, PEPAb, PEPAc, and PEPAd. Also described are nucleic acids and methods for making the engineered filamentous fungal cells.Type: ApplicationFiled: July 28, 2009Publication date: December 1, 2011Applicant: DANISCO US INC.Inventor: Huaming Wang
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Publication number: 20110283422Abstract: The present invention relates to isolated polypeptides having aspartic endopeptidase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: December 10, 2009Publication date: November 17, 2011Applicant: Novozymes, Inc.Inventors: Beth Nelson, Alfredo Lopez de Leon, Michael Rey
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Publication number: 20110283421Abstract: The present invention relates to isolated polypeptides having amylolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: November 2, 2009Publication date: November 17, 2011Applicant: Novozymes, Inc.Inventors: Paul Harris, Mark Wogulis
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Publication number: 20110281303Abstract: The present invention relates to isolated polypeptides having acetyl xylan esterase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: March 23, 2010Publication date: November 17, 2011Applicant: Novozymes A/SInventors: Johan Borjesson, Anders Viksoe-Nielsen, Peter Kamp Hansen
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Patent number: 8058053Abstract: The present invention relates to methods and genetically engineered methylotrophic yeast strains for producing glycoproteins with mammalian-like glycosylation. The present invention also relates to vectors useful for generating methylotrophic yeast strains capable of producing glycoproteins with mammalian-like glycosylation. Glycoproteins produced from the genetically engineered methylotrophic yeast strains are also provided.Type: GrantFiled: February 11, 2009Date of Patent: November 15, 2011Assignees: VIB, VZW, Universiteit Gent, Research Corporation Technologies, Inc.Inventors: Roland Contreras, Nico L. M. Callewaert, Wouter Vervecken, Vladimir Kaigorodov
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Publication number: 20110086133Abstract: The invention relates to the use of one or more pectinolytic enzyme(s) for the treatment of fruit or vegetable mash as well as a process for enzymatic treatment of fruit or vegetable mash comprising the step of adding one or more pectinolytic enzyme(s), wherein at least one pectinolytic enzyme is obtainable from Trichoderma reesei, as well as to a process for the preparation of a fruit or vegetable juice comprising the process for enzymatic treatment of fruit or vegetable mash. Moreover, the invention discloses recombinant DNA molecules encoding a polypeptide having endo-polygalacturonase activity, a polypeptide having exo-polygalacturonase activity, a polypeptide having exo-rhamnogalacturonase activity and a polypeptide having xylogalacturonase activity.Type: ApplicationFiled: May 22, 2009Publication date: April 14, 2011Applicant: AB ENZYMES GMBHInventors: Terhi Puranen, Bernhard Seiboth, Klaudija Milos, Wilfried Theiss, Jarno Kallio, Christian Kubicek, Jari Vehmaanperä
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Publication number: 20110053195Abstract: An inactive xylanase molecule for the recovery of xylanase activity in plant-derived material containing active xylanase enzyme(s) and xylanase inhibitors. The inactive xylanase molecule of binds to xylanase inhibitors in the plant-derived material, thereby allowing accurate measurement of xylanase enzyme activity of the enzyme contained in the plant-derived material. The invention further includes amino acid molecules depicted by SEQ ID NOS. 4 through 112, wherein the catalytically active sites of each of the amino acids have been modified resulting in inactive xylanase molecules. A method of production of the inactive xylanase molecules includes expression of the inactive xylanase molecule in microbial or eukaryal (e.g.Type: ApplicationFiled: June 12, 2007Publication date: March 3, 2011Applicant: SYNGENTA PARTICIPATIONS AGInventors: Michael William Bauer, Justin DeFontes
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Publication number: 20110053233Abstract: The present invention relates to methods of producing a C4 dicarboxylic acid, comprising: (a) cultivating a filamentous fungal host cell comprising a polynucleotide selected from the group consisting of a heterologous first polynucleotide encoding a C4 dicarboxylic acid transporter, a heterologous second polynucleotide encoding a malate dehydrogenase, and a heterologous third polynucleotide encoding a pyruvate carboxylase; wherein the filamentous fungal host cell is capable of secreting increased levels of the C4 dicarboxylic acid compared to the filamentous fungal host cell without the heterologous polynucleotide when cultivated under the same conditions; and (b) recovering the C4 dicarboxylic acid. The present invention also relates to methods for increasing C4 dicarboxylic acid production, filamentous fungal host cells and malate dehydrogenase variants.Type: ApplicationFiled: August 27, 2010Publication date: March 3, 2011Applicant: NOVOZYMES, INC.Inventors: STEPHEN BROWN, SHERYL LUTTRINGER, DEBBIE YAVER, ALAN BERRY
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Publication number: 20110054193Abstract: The present invention relates to a polypeptide with HMG-CoA reductase activity, to its polynucleotide congener and to a method for the production of a statin comprising over expression of said polypeptide.Type: ApplicationFiled: April 28, 2009Publication date: March 3, 2011Inventors: Marco Alexander Van Den Berg, Marcus Hans
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Publication number: 20110003333Abstract: The present invention relates to a method of producing a protein of interest comprising: (a) cultivating a mutant cell under conditions conducive for production of the protein wherein the mutant has a reduced or no expression of an endogenous polypeptide shown in SEQ ID NO: 2 compared to a parent host cell grown under the same conditions; and optionally (b) recovering the protein of interest.Type: ApplicationFiled: February 20, 2009Publication date: January 6, 2011Applicant: Novozymes A/SInventors: Mogens Trier Hansen, Birthe Marie Ravn, Leonardo De Maria
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Publication number: 20100323414Abstract: The invention provides methods of producing lipids by oleaginous microorganisms. The invention also provides genetically engineered oleaginous microorganisms and methods of cultivation for lipid production. Also provided are oils, fuels, oleochemicals, chemical precursors, and other compounds manufactured such microorganisms. Exemplary oleaginous microorganisms include oleaginous fungi and such oleaginous fungi that have been genetically modified.Type: ApplicationFiled: April 30, 2010Publication date: December 23, 2010Applicant: Solazyme, Inc.Inventors: Donald E. Trimbur, Chung-Soon Im, Harrison F. Dillon, Anthony G. Day, Scott Franklin, Anna Coragliotti
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Publication number: 20100323426Abstract: The present invention provides filamentous fungi that express a combination of heterologous and homologous polypeptides, polypeptide mixtures comprising a combination of heterologous and homologous polypeptides and methods of producing the polypeptide mixtures.Type: ApplicationFiled: June 5, 2008Publication date: December 23, 2010Inventors: Benjamin S. Bower, Edmund A. Larenas
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Publication number: 20100261259Abstract: The present invention provides a method for the recombinant expression of polypeptides originating from gram negative bacteria, in a fungal host suitable for industrial production. In a first aspect the present invention relates to a method for recombinant expression of a polypeptide from a gram negative bacterium in a fungal host cell, comprising the steps: i) providing a nucleic acid sequence encoding the polypeptide, said nucleic acid sequence comprising a first nucleic acid sequence encoding a fungal signal peptide and a second nucleic acid sequence encoding the polypeptide, having at least one modified codon, wherein the modification does not change the amino acid encoded by said codon and the nucleic acid sequence of said codon is different compared to the corresponding codon in the wild type nucleic acid sequence present in the said gram negative bacterium; ii) expressing the modified nucleic acid sequence in the fungal host.Type: ApplicationFiled: August 3, 2007Publication date: October 14, 2010Applicant: Novozymes A/SInventors: Tomoko Matsui, Pia Francke Johannesen, Shinobu Takagi, Thomas Agersten Poulsen, Noriko Tsutsumi, Zheng Liu, XiangYu Kong
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Publication number: 20100184138Abstract: The present invention provides filamentous fungi that express a combination of heterologous and homologous polypeptides, polypeptide mixtures comprising a combination of heterologous and homologous polypeptides and methods of producing the polypeptide mixtures.Type: ApplicationFiled: December 3, 2009Publication date: July 22, 2010Inventors: Benjamin Bower, Edmund Larenas
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Publication number: 20100173390Abstract: The present invention relates to isolated nucleic acid sequences encoding polypeptides having oxaloacetate hydrolase activity. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as recombinant methods for producing the polypeptides.Type: ApplicationFiled: March 8, 2010Publication date: July 8, 2010Applicant: Novozymes A/SInventors: Carsten Mailand Hjort, Henrik Pedersen
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Publication number: 20100143989Abstract: This invention relates to nitrilase mutants having improved nitrilase activity for converting 3-hydroxynitriles to 3-hydroxycarboxylic acids. More specifically, the Acidavorax facilis 72W (ATCC 55746) nitrilase gene was mutated using error-prone PCR and site-directed mutagenesis to create nitrilase enzymes having improved nitrilase activity for converting 3-hydroxynitriles (e.g., 3-hydroxybutyronitrile or 3-hydroxyvaleronitrile) to the corresponding 3-hydroxycarboxylic acids. A process using these improved mutants to produce the 3-hydroxycarboxylic acids is also provided.Type: ApplicationFiled: April 14, 2008Publication date: June 10, 2010Applicant: E. I. DU PONT DE NEMOURS AND COMPANYInventors: Mark S. Payne, Robert DiCosimo, Daniel P. O'Keefe
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Publication number: 20100119656Abstract: The invention relates to a DNA sequence that encodes a polypeptide with lysophospholipase activity and was isolated from Aspergillus and sequences derived therefrom, polypeptides with lysophospholipase activity encoded by these sequences as well as the use of these polypeptides for improving the filterability of syrups consisting of wheat starch and for related applications.Type: ApplicationFiled: September 21, 2007Publication date: May 13, 2010Applicant: AB Enzymes GmbHInventors: Khanh Q. Nguyen, Volker Marschner, Kornelia Titze, Bruno Winter
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Publication number: 20100048938Abstract: The present invention provides a microorganism containing a compactin biosynthesis gene and a gene for conversion of compactin into pravastatin. In a preferred example, said compactin biosynthesis gene is mIcA and/or mIcB and/or mIcC and/or mIcD and/or mIcE and/or mIcF and/or mIcG and/or mIcH and/or mIcR and said gene for conversion of compactin into pravastatin is a hydroxylase gene. Furthermore, the present invention provides a method for producing a compound of interest such as a statin. In a preferred example said statin is pravastatin.Type: ApplicationFiled: June 19, 2007Publication date: February 25, 2010Inventors: Marco Alexander Van Den Berg, Bernard Meijrink
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Publication number: 20100003365Abstract: The invention relates to a DNA sequence that encodes a polypeptide with phospholipase activity and was isolated from Aspergillus and sequences derived therefrom, polypeptides with phospholipase activity encoded by these sequences as well as the use of these polypeptides for degumming of vegetable oil, for the preparation of dough and/or bakery products, for the preparation of dairy products, for processing steps in the textile industry and for related applications.Type: ApplicationFiled: September 21, 2007Publication date: January 7, 2010Applicant: AB Enzymes GmbHInventors: Khanh Q. Nguyen, Volker Marschner, Kornelia Titze, Bruno Winter
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Publication number: 20090253173Abstract: The invention relates to a filamentous fungal cell (e.g., Aspergillus sp.) comprising at least one inactivated protease gene chosen from apsB, a homolog of apsB, cpsA, a homolog cpsA, and combinations thereof. Nucleic acids and methods for making the inactivated mutant filamentous fungal cells are provided as well as methods for using the cells for the altered production of endogenous or heterologous proteins of interest.Type: ApplicationFiled: March 26, 2009Publication date: October 8, 2009Applicant: Danisco US Inc., Genencor DivisionInventor: Huaming Wang
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Publication number: 20090203089Abstract: Bacterial polynucleotides and polypeptides are provided in which the polypeptides have a dehydrogenase activity, such as an alcohol dehydrogenase (ADH) activity, an uronate, a 4-deoxy-L-erythro-5-hexoseulose uronate (DEHU) ((4S,5S)-4,5 dihydroxy-2,6-dioxohexanoate) hydrogenase activity, a 2-keto-3-deoxy-D-gluconate dehydrogenase activity, a D-mannuronate hydrogenase activity, and/or a D-mannnonate dehydrogenase activity. Methods, enzymes, recombinant microorganism, and microbial systems are also provided for converting polysaccharides, such as those derived from biomass, into suitable monosaccharides or oligosaccharides, as well as for converting suitable monosaccharides or oligosaccharides into commodity chemicals, such as biofuels. Commodity chemicals produced by the methods described herein are also provided.Type: ApplicationFiled: January 28, 2009Publication date: August 13, 2009Applicant: BIO ARCHITECTURE LAB, INC.Inventor: Yuki Kashiyama
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Publication number: 20090169536Abstract: The invention relates to a recombinant DNA molecule, which, upon expression in a prokaryotic or eukaryotic host cell, encodes a polypeptide having phytase activity, wherein the recombinant DNA molecule comprises a DNA sequence selected from a) DNA sequences that have been obtained by variations of the mature wild-type E. coli phytase sequence, wherein at least one amino acid in the region of position 189 to 211 and/or an amino acid in the region of position 137 to 152 is mutated as compared to the wild-type sequence, b) DNA sequences having a homology of 70% to 100% to the sequences according to a), c) DNA sequences that are related to the sequences according to a) and b) due to the degeneracy of the genetic code, wherein the recombinant DNA molecule is, upon expression in a suitable host cell, associated with an increased activity of the thus encoded protein in the culture supernatant, as well as the proteins encoded by the same.Type: ApplicationFiled: October 14, 2005Publication date: July 2, 2009Applicant: AB ENZYMES GMBHInventors: Khanh Q. Nguyen, Bruno Winter
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Patent number: 7491510Abstract: The present invention relates to the use of a glucosidase II mutation to increase protein secretion in eukaryotic cells. The present invention relates further to the use of eukaryotic cells, comprising a mutant glucosidase II gene, possibly in combination with the expression of a recombinant ?-1,2-mannosidase gene and/or a recombinant N-acetylglucosaminyl-transferase gene, as a host for protein secretion.Type: GrantFiled: September 12, 2005Date of Patent: February 17, 2009Assignees: Vlaams Interuniversitair Institute voor Biotechnologie vzw, Universiteit GentInventors: Roland Contreras, Steven Geysens
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Patent number: 7291493Abstract: The present invention relates to codon-optimized xylanase coding sequences and the expression of xylanases in microbes and yeast. The invention further relates to using multiple copies of the xylanase expression construct for high levels of protein expression. The invention also relates to the use of xylanases as feed or food additives. The invention also relates to methods of expression of enzymes to increase thermotolerance by expressing them in organisms that glycosylate proteins compared to expression that the same enzyme without the glycosylation. Further, the invention relates to methods of preparing feed, enzyme feed additives, and methods of reducing the feed conversion ration or increasing weight gain of animals.Type: GrantFiled: December 20, 2004Date of Patent: November 6, 2007Assignee: Syngenta Participations AGInventors: Michael Bauer, Michael Richard Bedford, Derrick Allen Pulliam
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Patent number: 7198938Abstract: The present invention relates to methods of obtaining a mutant cell from a filamentous fungal parent cell, comprising: (a) obtaining mutant cells of the parent cell; (b) identifying the mutant cell which exhibits a more restricted colonial phenotype and/or a more extensive hyphal branching than the parent cell; and (c) identifying the mutant cell which has an improved property for production of a heterologous polypeptide than the parent cell, when the mutant and parent cells are cultured under the same conditions.Type: GrantFiled: June 30, 2004Date of Patent: April 3, 2007Assignee: Novozymes, Inc.Inventors: Jeffrey R. Shuster, John C. Royer
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Patent number: 7195901Abstract: The gene for the diacylglycerol acyltransferase (DGAT) from Ricinus communis L., the castor plant, has been isolated, cloned, and used to transform other plants and micro-organisms. When the gene is expressed in a heterologous system, the corresponding DGAT protein is active and shows unusual and selective action for hydroxylated fatty acyl glycerides. DGAT carries out the final step in castor oil biosynthesis, and is believed to be largely responsible for many of the attributes of castor oil, making it an excellent candidate for industrial uses. This invention makes it possible to enhance the oil-producing capacity of other plants and micro-organisms.Type: GrantFiled: June 3, 2004Date of Patent: March 27, 2007Assignee: The United States of America as represented by the Secretary of AgricultureInventors: Thomas A. McKeon, Xiaohua He, Jiann-Tsyh Lin
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Patent number: 7067300Abstract: The present invention provides an improved method for the biological production of 1,3-propanediol from a fermentable carbon source in a single microorganism. In one aspect of the present invention, an improved process for the conversion of glucose to 1,3-propanediol is achieved by the use of an E. coli transformed with the Klebsiella pneumoniae dha regulon genes dhaR, orfY, dhaT, orfX, orfW, dhaB1, dhaB2, dhaB3, and orfZ, all these genes arranged in the same genetic organization as found in wild type Klebsiella pneumoniae. In another aspect of the present invention, an improved process for the production of 1,3-propanediol from glucose using a recombinant E. coli containing genes encoding a G3PDH, a G3P phosphatase, a dehydratase, and a dehydratase reactivation factor compared to an identical process using a recombinant E. coli containing genes encoding a G3PDH, a G3P phosphatase, a dehydratase, a dehydratase reactivation factor and a 1,3-propanediol oxidoreductase (dhaT).Type: GrantFiled: October 21, 2002Date of Patent: June 27, 2006Assignee: E. I. du Pont de Nemours and CompanyInventors: Mark Emptage, Sharon L. Haynie, Lisa A. Laffend, Jeff P. Pucci, Gregory Marshall Whited
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Patent number: 6794180Abstract: The present invention relates to methods of obtaining a mutant cell from a filamentous fungal parent cell, comprising: (a) obtaining mutant cells of the parent cell; (b) identifying the mutant cell which exhibits a more restricted colonial phenotype and/or a more extensive hyphal branching than the parent cell; and (c) identifying the mutant cell which has an improved property for production of a heterologous polypeptide than the parent cell, when the mutant and parent cells are cultured under the same conditions.Type: GrantFiled: February 25, 2003Date of Patent: September 21, 2004Assignee: Novozymes Biotech, Inc.Inventors: Jeffrey R. Shuster, John C. Royer
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Publication number: 20030224482Abstract: Novel vectors are disclosed for expressing and secreting heterologous polypeptides from filamentous fungi. Such vectors are used in novel processes to express and secrete such heterologous polypeptides. The vectors used for transforming a filamentous fungus to express and secrete a heterologous polypeptide include a DNA sequence encoding a heterologous polypeptide and a DNA sequence encoding a signal sequence which is functional in a secretory system in a given filamentous fungus and which is operably linked to the sequence encoding the heterologous polypeptide. Such signal sequences may be the signal sequence normally associated with the heterologous polypeptides or may be derived from other sources. The vector may also contain DNA sequences encoding a promoter sequence which is functionally recognized by the filamentous fungus and which is operably linked to the DNA sequence encoding the signal sequence.Type: ApplicationFiled: February 6, 2003Publication date: December 4, 2003Applicant: Genencor International, Inc.Inventors: Randy Michael Berka, Daniel Cullen, Gregory Lawrence Gray, Kirk James Hayenga, Virgil Bryan Lawlis
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Publication number: 20030134353Abstract: It is an object of the present invention to provide fungal host organisms capable of expressing recombinant proteins while at the same time exhibiting satisfactory growth characteristics. It is a further object to provide—in a single fungal host organism—the characteristic of homogeneous growth and low viscosity typically associated with yeast organisms, and the capability for high protein secretion normally associated with filamentous fungi. It is a yet further object of the invention to provide useful tools for genetic analysis in zygomycetes, including dimorphic zygomycetes. Accordingly, the present invention relates to a recombinant, fungal cell or dimorphic fungal cell comprising a regulatable expression of a regulator of morphology. The nucleotide sequence encoding the at least one regulator of morphology is operably linked to an expression signal not natively associated therewith.Type: ApplicationFiled: March 8, 2002Publication date: July 17, 2003Applicant: Bioteknologisk InstitutInventors: Anne Mette Wolff, Karen Fuglede Appel, Jesper Breum Petersen, Ulla Poulsen, Jose Arnau, Mette Dorph Jacobsen
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Patent number: 6436643Abstract: A process for site-directed integration of multiple copies of a gene in a mould is provided, which comprises transforming a mould cell containing in its chromosomal DNA a restriction site for a rare-cutting endonuclease, e.g., I-Scel, preferably introduced at a desired locus, e.g., within a selectable marker gene or in the neighborhood thereof, with a piece of DNA comprising multiple copies of at least one expressible gene comprising at least one structural gene encoding a desired protein, surrounded by two DNA fragments homologous to part of the DNA upstream and downstream, and in the neighborhood, of said restriction site, while during the transformation of the mould the presence of the rare-cutting endonuclease is provided, followed by selecting or screening for a mould cell in which the multiple gene copies of said expressible gene are inserted into the chromosomal DNA of the mould.Type: GrantFiled: August 2, 2000Date of Patent: August 20, 2002Assignee: Unilever Patent Holdings BVInventors: Marcellus Johannes Augustinus de Groot, Alida Godelieve Maria Beijersbergen, Wouter Musters
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Patent number: 6432672Abstract: The present invention relates to filamentous fungi that comprise in their genomes at least two substantially homologous DNA domains which are suitable for integration of one or more copies of a recombinant DNA molecule and wherein at least two of these DNA domains comprise an integrated copy of a recombinant DNA molecule. The invention also relates to methods for preparing such filamentous fungi and for further multiplying the DNA domains with integrated recombinant DNA molecules through gene conversion or amplification.Type: GrantFiled: January 24, 2000Date of Patent: August 13, 2002Inventors: Gerardus Cornelis Maria Selten, Bart Willem Swinkels, Roelof Ary Lans Bovenberg
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Patent number: 6379928Abstract: Novel vectors are disclosed for expressing and secreting heterologous polypeptides from filamentous fungi. Such vectors are used in novel processes to express and secrete such heterologous polypeptides. The vectors used for transforming a filamentous fungus to express and secrete a heterologous polypeptide include a DNA sequence encoding a heterologous polypeptide and a DNA sequence encoding a signal sequence which is functional in a secretory system in a given filamentous fungus and which is operably linked to the sequence encoding the heterologous polypeptide. Such signal sequences may be the signal sequence normally associated with the heterologous polypeptides or may be derived from other sources. The vector may also contain DNA sequences encoding a promoter sequence which is functionally recognized by the filamentous fungus and which is operably linked to the DNA sequence encoding the signal sequence.Type: GrantFiled: December 10, 1999Date of Patent: April 30, 2002Assignee: Genencor International, Inc.Inventors: Randy Michael Berka, Daniel Cullen, Gregory Lawrence Gray, Kirk James Hayenga, Virgil Bryan Lawlis
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Patent number: 6171817Abstract: Novel vectors are disclosed for expressing and secreting heterologous polypeptides from filamentous fungi. Such vectors are used in novel processes to express and secrete such heterologous polypeptides. The vectors used for transforming a filamentous fungus to express and secrete a heterologous polypeptide include a DNA sequence encoding a heterologous polypeptide and a DNA sequence encoding a signal sequence which is functional in a secretory system in a given filamentous fungus and which is operably linked to the sequence encoding the heterologous polypeptide. Such signal sequences may be the signal sequence normally associated with the heterologous polypeptides or may be derived from other sources. The vector may also contain DNA sequences encoding a promoter sequence which is functionally recognized by the filamentous fungus and which is operably linked to the DNA sequence encoding the signal sequence.Type: GrantFiled: November 24, 1997Date of Patent: January 9, 2001Assignee: Genencor International, Inc.Inventors: Randy Michael Berka, Daniel Cullen, Gregory Lawrence Gray, Kirk James Hayenga, Virgil Bryan Lawlis