Rhizopus Patents (Class 435/254.9)
  • Patent number: 10435724
    Abstract: Improvement of C4 dicarboxylic acid productivity in a host cell. A polypeptide consisting of an amino acid sequence represented by SEQ ID No: 2 or an amino acid sequence having an identity of at least 90% with the amino acid sequence represented by SEQ ID No: 2.
    Type: Grant
    Filed: October 12, 2016
    Date of Patent: October 8, 2019
    Assignee: Kao Corporation
    Inventor: Jitsuro Kaneda
  • Publication number: 20150087028
    Abstract: The invention relates to recombinant expression of variant forms of M. thermophila CBH1a and homologs thereof, having improved thermoactivity, specific activity, and other desirable properties. Also provided are methods for producing ethanol and other valuable organic compounds by combining cellobiohydrolase variants with cellulosic materials.
    Type: Application
    Filed: March 12, 2013
    Publication date: March 26, 2015
    Inventors: Behnaz Behrouzian, Xinkai Xie, Kui Chan, Xiyun Zhang, Vesna Mitchell, Douglas A. Hattendorf
  • Publication number: 20150064751
    Abstract: The embodiments described herein pertain to cells, and methods for preparing cells, that can be used as biocatalysts by altering enzymes that compete for a substrate or product of a pathway of interest such that the targeted enzyme is sensitive to a site-specific protease, which protease is expressed but relocated in the cell to a site where it is not in contact with the targeted enzyme in the intact cell. Upon cell lysis, the protease contacts the target enzyme, which is then inactivated by protease cleavage.
    Type: Application
    Filed: November 14, 2014
    Publication date: March 5, 2015
    Applicants: The Board of Trustees of the Leland Stanford Junior University, GreenLight Biosciences, Inc.
    Inventor: James R. Swartz
  • Publication number: 20150050708
    Abstract: Provided herein are non-naturally occurring microbial organisms having a formaldehyde fixation pathway and a formate assimilation pathway, which can further include a methanol metabolic pathway, a methanol oxidation pathway, a hydrogenase and/or a carbon monoxide dehydrogenase. These microbial organisms can further include a butadiene, 1,3-butanediol, crotyl alcohol or 3-buten-2-ol pathway. Additionally provided are methods of using such microbial organisms to produce butadiene, 1,3-butanediol, crotyl alcohol or 3-buten-2-ol.
    Type: Application
    Filed: March 14, 2014
    Publication date: February 19, 2015
    Applicant: Genomatica, Inc.
    Inventors: Anthony P. BURGARD, Robin E. OSTERHOUT, Priti PHARKYA, Stefan ANDRAE
  • Publication number: 20150024009
    Abstract: The invention provides an isolated genetically modified non-mammalian organism, wherein the activity of acyl-CoA:sterol acyltransferase/sterol O-acyltransferase (EC 2.3.1.26) and/or diacylglycerol acyltransferase/diacylglycerol O-acyltranferase (EC 2.3.1.20) and/or lecithin cholesterol acyl transferase/phospholipid: diacylglycerol acyltransferase (EC 2.3.1.158) and/or acyl CoA-wax alcohol acyltransferase (EC 2.3.1.75) is reduced or abolished in comparison with a corresponding wildtype organism, methods of use of such an organism, shuttle vehicles for making such an organism and methods for producing such an organism.
    Type: Application
    Filed: September 19, 2014
    Publication date: January 22, 2015
    Applicant: ORGANOBALANCE GMBH
    Inventors: Christine Lang, Andreas Raab
  • Publication number: 20150010961
    Abstract: The present invention relates to a family 5 glycoside hydrolase variant having endoglucanase activity, polynucleotides encoding the family 5 glycoside hydrolase variant, vectors, host cells comprising the polynucleotides, and methods for using the family 5 glycoside hydrolase variant.
    Type: Application
    Filed: September 25, 2014
    Publication date: January 8, 2015
    Inventors: John Poland, Justin T. Stege
  • Publication number: 20150010973
    Abstract: The invention relates to fungal strains having at least one genetic modification which leads to a reduction of the activity of at least one fungal carboxylic acid transporter and to a method for producing or using said fungal strains.
    Type: Application
    Filed: December 12, 2012
    Publication date: January 8, 2015
    Applicant: THYSSENKRUPP INDUSTRIAL SOLUTIONS AG
    Inventors: Andreas Aurich, Martina Holz, Anne Kretzschmar, Christina Otto, Gerold Barth, Isabel Waengler, Roland Arno Müller
  • Publication number: 20150004660
    Abstract: Embodiments of the invention relate to the enzymatic conversion of bioderived feedstocks to commercially valuable chemicals. The enzymatic conversions of the embodiments of the invention offer the potential for lower cost routes to these value-added chemicals. Some of the chemicals that are useful include nylon intermediates such as caprolactam, adipic acid, 1,6-hexamethylene diamine; butanediols such as 1,4-butanediol, 1,3-butanediol, and 2,3-butanediol; butanols such as 1-butanol, and 2-butanol; succinic acid, butadiene, isoprene, and 3-hydroxypropanoic acid.
    Type: Application
    Filed: September 18, 2014
    Publication date: January 1, 2015
    Inventors: Paul S. Pearlman, Changlin Chen, Adriana Leonora Botes
  • Publication number: 20140371417
    Abstract: The invention provides non-naturally occurring microbial organisms having a 4-hydroxybutyrate, gamma-butyrolactone, 1,4-butanediol, 4-hydroxybutanal, 4-hydroxybutyryl-CoA and/or putrescine pathway and being capable of producing 4-hydroxybutyrate, wherein the microbial organism comprises one or more genetic modifications. The invention additionally provides methods of producing 4-hydroxybutyrate, gamma-butyrolactone, 1,4-butanediol, 4-hydroxybutanal, 4-hydroxybutyryl-CoA and/or putrescine or related products using the microbial organisms.
    Type: Application
    Filed: April 25, 2014
    Publication date: December 18, 2014
    Applicant: Genomatica, Inc.
    Inventors: Priti PHARKYA, Anthony P. BURGARD, Stephen J. VAN DIEN, Robin E. OSTERHOUT, Mark J. BURK, John D. TRAWICK, Michael P. KUCKINSKAS, Brian STEER
  • Publication number: 20140329916
    Abstract: Provided herein is a non-naturally occurring microbial organism having a methanol metabolic pathway that can enhance the availability of reducing equivalents in the presence of methanol. Such reducing equivalents can be used to increase the product yield of organic compounds produced by the microbial organism, such as adipate, 6-aminocaproate, hexamethylenediamine or caprolactam. Also provided herein are methods for using such an organism to produce adipate, 6-aminocaproate, hexamethylenediamine or caprolactam.
    Type: Application
    Filed: December 16, 2013
    Publication date: November 6, 2014
    Applicant: Genomatica, Inc.
    Inventors: Anthony P. Burgard, Robin E. Osterhout, Stephen J. Van Dien, Cara Ann Tracewell, Priti Pharkya, Stefan Andrae
  • Publication number: 20140287471
    Abstract: The present disclosure relates to variant CBH I polypeptides that have reduced product inhibition, and compositions, e.g., cellulase compositions, comprising variant CBH I polypeptides. The variant CBH I polypeptides and related compositions can be used in variety of agricultural and industrial applications. The present disclosure further relates to nucleic acids encoding variant CBH I polypeptides and host cells that recombinantly express the variant CBH I polypeptides.
    Type: Application
    Filed: October 5, 2012
    Publication date: September 25, 2014
    Applicant: Bp Corporation North America Inc.
    Inventors: Sarah Richardson Hanson, Justin T. Stege, Cecilia Cheng, Peter Luginbuhl
  • Publication number: 20140242655
    Abstract: Embodiments of the present invention relate to methods for the biosynthesis of di- or trifunctional C7 alkanes in the presence of isolated enzymes or in the presence of a recombinant host cell expressing those enzymes. The di- or trifunctional C7 alkanes are useful as intermediates in the production of nylon-7, nylon-7,x, nylon-x,7, and polyesters.
    Type: Application
    Filed: June 29, 2012
    Publication date: August 28, 2014
    Applicant: INVISTA TECHNOLOGIES S.A R.L.
    Inventors: Paul S. Pearlman, Changlin Chen, Adriana Leonora Botes, Alex Van Eck Conradie, Benjamin D. Herzog
  • Publication number: 20140234925
    Abstract: The present invention relates to methods of producing a C4 dicarboxylic acid, comprising: (a) cultivating a filamentous fungal host cell comprising a polynucleotide selected from the group consisting of a heterologous first polynucleotide encoding a C4 dicarboxylic acid transporter, a heterologous second polynucleotide encoding a malate dehydrogenase, and a heterologous third polynucleotide encoding a pyruvate carboxylase; wherein the filamentous fungal host cell is capable of secreting increased levels of the C4 dicarboxylic acid compared to the filamentous fungal host cell without the heterologous polynucleotide when cultivated under the same conditions; and (b) recovering the C4 dicarboxylic acid. The present invention also relates to methods for increasing C4 dicarboxylic acid production, filamentous fungal host cells and malate dehydrogenase variants.
    Type: Application
    Filed: May 5, 2014
    Publication date: August 21, 2014
    Applicant: Novozymes, Inc.
    Inventors: Stephen Brown, Sheryl Luttringer, Debbie Yaver, Alan Berry
  • Publication number: 20140186884
    Abstract: This disclosure relates to novel xylose isomerases and their uses, particularly in fermentation processes that employ xylose-containing media.
    Type: Application
    Filed: July 23, 2013
    Publication date: July 3, 2014
    Applicant: BP Corporation North Americca Inc.
    Inventors: David Neal Nunn, JR., Peter Luginbuhl, Ling Li, Adam Martin Burja, John Peter Flash Bartnek
  • Publication number: 20140147894
    Abstract: The present disclosure relates to variant CBH I polypeptides that have reduced product inhibition, and compositions, e.g., cellulase compositions, comprising variant CBH I polypeptides. The variant CBH I polypeptides and related compositions can be used in variety of agricultural and industrial applications. The present disclosure further relates to nucleic acids encoding variant CBH I polypeptides and host cells that recombinantly express the variant CBH I polypeptides.
    Type: Application
    Filed: October 6, 2011
    Publication date: May 29, 2014
    Applicant: BP Corporation North America Inc.
    Inventors: Justin T. Stege, Alexander Varvak, John Poland, Chris S. Lyon, Shaun Healey, Peter Luginhuhl
  • Publication number: 20140141467
    Abstract: The present invention demonstrates the utility of carbonic acid amides such as urea or its derivatives, carbamates, carbodiimides & thiocarbamides as nitrogenous supplements in fermentation media for production of recombinant proteins to achieve enhanced bioconversion rates and peptides like insulin and insulin analogues, exendin and enzymes such as lipase using methanol inducible fungal expression systems such as Pichia.
    Type: Application
    Filed: January 23, 2014
    Publication date: May 22, 2014
    Applicant: Biocon Limited
    Inventors: Sanjay Tiwari, Mukesh Babuappa Patale, Saurabh Garg, Mayank Kumar Garg, Sulekha Joshi, Chittnalli Ramegowda Naveen Kumar, Bimal Kumar, Anuj Goel, Harish Iyer
  • Publication number: 20140134707
    Abstract: The present invention is related to a fungal serine protease enzyme, which comprises an amino acid sequence the mature Fa_RF7182 enzyme having an amino acid sequence of SEQ ID NO: 18. The serine protease is obtainable from Fusarium acuminatum, more preferably from the deposited strain CBS 124084. Also disclosed are nucleic acid sequences encoding said protease, such as plasmid pALK2530 comprising the nucleotide sequence SEQ ID NO:12 deposited in Escherichia coli RF7803 under accession number DSM 22208 and plasmid pALK2531 comprising the full-length gene SEQ ID NO: 13 deposited in E. coli RF7879 under accession number DSM 22209, as well as fungal hosts, such as Trichoderma. Said protease is useful as an enzyme preparation applicable in detergent compositions and for treating fibers, for treating wool, for treating hair, for treating leather, for treating food or feed, or for any applications involving modification, degradation or removal of proteinaceous material.
    Type: Application
    Filed: October 30, 2013
    Publication date: May 15, 2014
    Applicant: AB Enzymes Oy
    Inventors: Kari Juntunen, Leena Valtakari, Susanna Makinen, Jarno Kallio, Jari Vehmaanpera, Pentti Ojapalo, Marja Paloheimo, George Szakacs
  • Publication number: 20140134706
    Abstract: The present invention is related to a fungal serine protease enzyme, which comprises an amino acid sequence of the mature Fe_RF6318 enzyme having an amino acid sequence of SEQ ID NO: 15. The serine protease is obtainable from Fusarium equiseti, more preferably from the deposited strain CBS 119568. Also disclosed are nucleic acid sequences encoding said protease, such as plasmid pALK2521 comprising the nucleotide sequence SEQ ID NO:9 deposited in E. coli RF7664 under accession number DSM 22171 and plasmid pALK2529 comprising the full-length gene SEQ ID NO: 10 deposited in E. coli RF7800 under accession number DSM 22172. Said protease is useful as an enzyme preparation applicable in detergent compositions and for treating fibers, for treating wool, for treating hair, for treating leather, for treating food or feed, or for any applications involving modification, degradation or removal of proteinaceous material.
    Type: Application
    Filed: October 29, 2013
    Publication date: May 15, 2014
    Applicant: AB Enzymes Oy
    Inventors: Kari Juntunen, Leena Valtakari, Susanna Makinen, Jarno Kallio, Jari Vehmaanpera, Pentti Ojapalo, Marja Paloheimo
  • Publication number: 20140134688
    Abstract: The present invention relates to isolated polypeptides having bicarbonate transporter activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, and methods of producing C4-dicarboxylic acids, such as malic acid.
    Type: Application
    Filed: January 21, 2014
    Publication date: May 15, 2014
    Applicant: Novozymes, Inc.
    Inventors: Amanda Fischer, Stephen Brown, Sheryl Luttringer
  • Publication number: 20140113352
    Abstract: The present invention provides compositions and methods for the expression of recombinant ?-glucosidase variants, as well as their use in the production of fermentable sugars from cellulosic biomass.
    Type: Application
    Filed: December 19, 2013
    Publication date: April 24, 2014
    Applicant: Codexis, Inc.
    Inventors: Dipnath Baidyaroy, Louis Clark, Lisa M. Newman
  • Publication number: 20140065678
    Abstract: The invention relates to recombinant expression of a variant form of a fungal C1 strain ?-glucosidase. The invention also relates to the generation of fermentable sugars from biomass and the production of biofuels by fermentation of the sugars using genetically modified organisms expressing the ?-glucosidase variant. The invention provides methods for producing a fermentable sugar, such as glucose, from cellobiose by contacting cellobiose with a recombinant ?-glucosidase variant protein, such as a variant protein secreted by a recombinant host cell into culture medium. Methods of the invention may be used for conversion of a biomass substrate to a fermentable sugar, and ultimately to ethanol or other biofuel.
    Type: Application
    Filed: November 8, 2013
    Publication date: March 6, 2014
    Applicant: Codexis, Inc.
    Inventors: Jie Yang, Xiyun Zhang, Attila Andor
  • Publication number: 20140065693
    Abstract: The present invention relates to the production of sugar hydrolysates from cellulosic material. The method may be used, for example for producing fermentable sugars for the production of bioethanol from lignocellulosic material. Cellulolytic enzymes and their production by recombinant technology are described, as well as uses of the enzymes and enzyme preparations.
    Type: Application
    Filed: October 9, 2013
    Publication date: March 6, 2014
    Applicant: ROAL OY
    Inventors: Terhi Puranen, Sauli Toikka, Kim Langfelder, Jari Vehmaanpera
  • Publication number: 20140045230
    Abstract: The present invention relates to a recombinant fungus comprising an enzyme which catalysts the conversion of malic acid to fumaric acid in the cytosol. The invention further relates to a process for the production of a dicarboxylic acid such as fumaric acid and succinic acid, wherein the recombinant fungus is used.
    Type: Application
    Filed: October 21, 2013
    Publication date: February 13, 2014
    Applicant: DSM IP ASSETS B.V.
    Inventors: Rene VERWAAL, Liang WU, Robbertus Antonius DAMVELD, Cornelis Maria Jacobus SAGT
  • Publication number: 20140030779
    Abstract: The invention provides non-naturally occurring microbial organisms having a 4-hydroxybutyrate pathway and being capable of producing 4-hydroxybutyrate, wherein the microbial organism comprises one or more genetic modifications. The invention additionally provides methods of producing 4-hydroxybutyrate or related products using the microbial organisms.
    Type: Application
    Filed: June 3, 2013
    Publication date: January 30, 2014
    Applicant: GENOMATICA, INC.
    Inventors: Priti PHARKYA, Anthony P. BURGARD, Stephen J. VAN DIEN, Robin E. OSTERHOUT, Mark J. BURK, John D. TRAWICK, Michael P. KUCHINSKAS, Brian STEER
  • Publication number: 20130316426
    Abstract: The invention provides a method of producing acrylic acid. The method includes contacting fumaric acid with a sufficient amount of ethylene in the presence of a cross-metathesis transformation catalyst to produce about two moles of acrylic acid per mole of fumaric acid. Also provided is an acrylate ester. The method includes contacting fumarate diester with a sufficient amount of ethylene in the presence of a cross-metathesis transformation catalyst to produce about two moles of acrylate ester per mole of fumarate diester. An integrated process for process for producing acrylic acid or acrylate ester is provided which couples bioproduction of fumaric acid with metathesis transformation. An acrylic acid and an acrylate ester production also is provided.
    Type: Application
    Filed: May 3, 2013
    Publication date: November 28, 2013
    Applicant: Genomatica, Inc.
    Inventor: Genomatica, Inc.
  • Publication number: 20130288310
    Abstract: The invention relates to C1 lignocellulose degradation enzyme nucleic acid and protein sequences and expression of recombinant C1 lignocellulose degradation enzymes. The invention provides methods for degrading a cellulosic biomass by contacting the biomass with a recombinant C1 lignocellulose degradation enzyme of the invention.
    Type: Application
    Filed: August 22, 2011
    Publication date: October 31, 2013
    Applicant: Codexis, Inc
    Inventors: Louis Clark, Dipnath Baidyaroy
  • Publication number: 20130244292
    Abstract: The present invention relates to a family 5 glycoside hydrolase variant having endoglucanase activity, polynucleotides encoding the family 5 glycoside hydrolase variant, vectors, host cells comprising the polynucleotides, and methods for using the family 5 glycoside hydrolase variant.
    Type: Application
    Filed: March 15, 2013
    Publication date: September 19, 2013
    Applicant: BP Corporation North America Inc.
    Inventors: John Poland, Justin T. Stege
  • Publication number: 20130224807
    Abstract: The invention relates to methods for enriching monomer content in a cycloalkane oxidation process mixed organic waste stream. In particular, the methods involve combining a biocatalyst with a mixed organic waste stream from a cycloalkane oxidation process, and enzymatically converting dimeric and/or oligomeric components of said waste stream into monomeric components. The methods may enrich the content of diacids, adipic acid, and/or other ?,?-difunctional C6 alkanes in the mixed organic waste stream. Additionally, the treated mixed organic waste streams may have improved burning efficiency.
    Type: Application
    Filed: June 15, 2012
    Publication date: August 29, 2013
    Applicant: INVISTA North America S.a r.l.
    Inventors: Paul S. Pearlman, Changlin Chen, Adriana L. Botes
  • Publication number: 20130203128
    Abstract: In alternative embodiments, the invention provides polypeptides having a lignocellulolytic (lignocellulosic) activity, e.g., a ligninolytic and cellulolytic activity, including, e.g., a glycosyl hydrolase, a cellulase, an endoglucanase, a cellobiohydrolase (cbhl) (e.g., an exo-cellobiohydrolase, e.g., having an “exo” activity that can processively release cellobiose units ?-1,4 glucose-glucose disaccharide), a beta-glucosidase, a xylanase, a mannanse, a xylosidase (e.g., a (?-xylosidase) and/or an arabinofuranosidase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In one embodiment, the invention provides thermostable and thermotolerant forms of polypeptides of the invention. The polypeptides and nucleic acids of the invention are used in a variety of pharmaceutical, agricultural and industrial contexts; for example, as enzymes for the bioconversion of a biomass, e.g.
    Type: Application
    Filed: October 6, 2011
    Publication date: August 8, 2013
    Applicant: BP Corporation North America Inc,
    Inventors: Justin T. Stege, Alexander Varvak, John Poland, Chris S. Lyon, Shaun Healey, Peter Luginbuhl
  • Publication number: 20130189751
    Abstract: The invention provides non-naturally occurring microbial organisms comprising a 1,4-butanediol (BDO), 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine pathway comprising at least one exogenous nucleic acid encoding a BDO, 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine pathway enzyme expressed in a sufficient amount to produce BDO, 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine and further optimized for expression of BDO. The invention additionally provides methods of using such microbial organisms to produce BDO, 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine.
    Type: Application
    Filed: January 9, 2013
    Publication date: July 25, 2013
    Applicant: Genomatica, Inc.
    Inventors: Robert HASELBECK, John D. TRAWICK, Wei NIU, Anthony P. BURGARD
  • Publication number: 20130143291
    Abstract: The present invention relates to methods for producing monoterpenes, particularly tricyclene, by culturing microbial organisms that express a terpene synthase and optionally a prenyl transferase.
    Type: Application
    Filed: March 30, 2011
    Publication date: June 6, 2013
    Applicant: Codexis, Inc.
    Inventors: Robert McDaniel, Galit Meshulam-Simon, Oscar Alvizo, Xiyun Zhang
  • Publication number: 20130144029
    Abstract: The invention provides non-naturally occurring microbial organisms containing caprolactone pathways having at least one exogenous nucleic acid encoding a butadiene pathway enzyme expressed in a sufficient amount to produce caprolactone. The invention additionally provides methods of using such microbial organisms to produce caprolactone by culturing a non-naturally occurring microbial organism containing caprolactone pathways as described herein under conditions and for a sufficient period of time to produce caprolactone.
    Type: Application
    Filed: November 2, 2012
    Publication date: June 6, 2013
    Inventors: Anthony P. Burgard, Robin E. Osterhout, Priti Pharkya, Mark J. Burk
  • Publication number: 20130137149
    Abstract: The invention is directed to a genetically modified microorganism for the extracellular production of free fatty acids and esters thereof, wherein said microorganism is characterised by a modified lipid biosynthesis metabolic pathway: for example reduced fatty acyl-coA synthetase activity that enables the microorganism to over-produced and secrete of esters of fatty acids (Biodiesel) into the surrounding medium, using one or more of: glucose, starch, lignocellulose and a glycerol-based substrate, as a carbon source. The invention further provides a method for the extracellular production of free fatty acids and esters thereof, comprising the use of said genetically modified organism, and a growth medium adapted for said method.
    Type: Application
    Filed: June 20, 2011
    Publication date: May 30, 2013
    Inventors: Ambareesh Govind Phadnavis, Peter Ruhdal Jensen
  • Publication number: 20130122563
    Abstract: The invention provides non-naturally occurring microbial organisms containing an alkene pathway having at least one exogenous nucleic acid encoding an alkene pathway enzyme expressed in a sufficient amount to convert an alcohol to an alkene. The invention additionally provides methods of using such microbial organisms to produce an alkene, by culturing a non-naturally occurring microbial organism containing an alkene pathway as described herein under conditions and for a sufficient period of time to produce an alkene.
    Type: Application
    Filed: September 14, 2012
    Publication date: May 16, 2013
    Inventors: Mark J. Burk, Robin E. Osterhout
  • Publication number: 20130109064
    Abstract: The invention provides non-naturally occurring microbial organisms containing 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol pathways comprising at least one exogenous nucleic acid encoding a butadiene pathway enzyme expressed in a sufficient amount to produce 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol. The invention additionally provides methods of using such microbial organisms to produce 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol, by culturing a non-naturally occurring microbial organism containing 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol pathways as described herein under conditions and for a sufficient period of time to produce 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol.
    Type: Application
    Filed: August 17, 2012
    Publication date: May 2, 2013
    Inventors: Robin E. Osterhout, Anthony P. Burgard, Mark J. Burk
  • Publication number: 20130102042
    Abstract: The invention refers to fungal cells, and especially to oleaginous fungal cells that have been genetically modified to produce enzymes of the pyruvate dehydrogenase bypass route to enhance their lipid production. Especially the cells are modified to over-express genes encoding pyruvate decarboxylase (PDC), acetaldehyde dehydrogenase (ALD) and/or acetyl-CoA synthetase (ACS), optionally together with a gene encoding diacylglycerol acyltransferase (DAT), or to express genes encoding PDC together with ALD and/or ACS. Methods of producing lipids, biofuels and lubricants using the modified fungi are also disclosed as well as expression cassettes useful therein. A new enzyme having phosholipid: diacylglycerol acyltransferase (PDAT)activity it and a polynucleotide encoding it are also disclosed, which are useful in the lipid production. A recombinant Cryptococcus cell and its construction is described.
    Type: Application
    Filed: June 21, 2011
    Publication date: April 25, 2013
    Applicant: TEKNOLOGIAN TUTKIMUSKESKUS VTT
    Inventors: Kari Koivuranta, Laura Ruohonen, Merja Penttilä
  • Publication number: 20130089902
    Abstract: The present invention relates to methods of producing C4 dicarboxylic acids, such as malic acid, comprising: (a) cultivating a host cell comprising a polynucleotide encoding a C4 dicarboxylic acid transporter; and (b) recovering the C4 dicarboxylic acid. The present invention also relates to methods for increasing C4 dicarboxylic acid production, as well as host cells comprising the polynucleotides.
    Type: Application
    Filed: June 2, 2011
    Publication date: April 11, 2013
    Applicant: Novozymes, Inc.
    Inventors: Sarah McFarland, Amanda Fischer
  • Publication number: 20130065279
    Abstract: The invention provides a non-naturally occurring microbial organism having a methacrylic acid, methacrylate ester, 3-hydroxyisobutyrate and/or 2-hydroxyisobutyrate pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in a methacrylic acid pathway. The invention additionally provides a method for producing methacrylic acid, methacrylate ester, 3-hydroxyisobutyrate and/or 2-hydroxyisobutyrate. The method can include culturing methacrylic acid, methacrylate ester, 3-hydroxyisobutyrate and/or 2-hydroxyisobutyrate producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding a methacrylic acid pathway enzyme in a sufficient amount to produce methacrylic acid, methacrylate ester, 3-hydroxyisobutyrate and/or 2-hydroxyisobutyrate, under conditions and for a sufficient period of time to produce methacrylic acid, methacrylate ester, 3-hydroxyisobutyrate and/or 2-hydroxyisobutyrate.
    Type: Application
    Filed: March 30, 2012
    Publication date: March 14, 2013
    Applicant: Genomatica, Inc.
    Inventors: Mark J. BURK, Anthony P. Burgard, Robin E. Osterhout, Jun Sun, Priti Pharkya
  • Publication number: 20130066035
    Abstract: Provided herein are non-naturally occurring eukaryotic organisms that can be engineered to produce and increase the availability of cytosolic acetyl-CoA. Also provided herein are non-naturally occurring eukaryotic organisms having a 1,3-butanediol (1,3-BDO) pathway. and methods of using such organisms to produce 1,3-BDO.
    Type: Application
    Filed: September 7, 2012
    Publication date: March 14, 2013
    Applicant: Genomatica, Inc.
    Inventors: Anthony P. Burgard, Mark J. Burk, Robin E. Osterhout, Priti Pharkya, Jingyi Li
  • Publication number: 20130059353
    Abstract: Provided herein are host cells comprising carbonic anhydrase activity, wherein the cells are capable of producing C4-dicarboxylic acid. Also provided are methods of producing C4-dicarboxylic acid comprising (a) cultivating the host cells having carbonic anhydrase activity in a medium under suitable conditions to produce C4-dicarboxylic acid; and (b) recovering the C4-dicarboxylic acid.
    Type: Application
    Filed: August 17, 2012
    Publication date: March 7, 2013
    Applicant: NOVOZYMES, INC.
    Inventors: Sarah McFarland, Stephen Brown, Sheryl Luttringer
  • Publication number: 20130029381
    Abstract: The invention provides non-naturally occurring microbial organisms comprising a 1,4-butanediol (BDO), 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine pathway comprising at least one exogenous nucleic acid encoding a BDO, 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine pathway enzyme expressed in a sufficient amount to produce BDO, 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine and further optimized for expression of BDO. The invention additionally provides methods of using such microbial organisms to produce BDO, 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine.
    Type: Application
    Filed: April 13, 2012
    Publication date: January 31, 2013
    Applicant: GENOMATICA, INC.
    Inventors: Robert Haselbeck, John D. Trawick, Wei Niu, Anthony P. Burgard
  • Publication number: 20130011891
    Abstract: The invention provides non-naturally occurring microbial organisms having a butadiene or crotyl alcohol pathway. The invention additionally provides methods of using such organisms to produce butadiene or crotyl alcohol.
    Type: Application
    Filed: June 19, 2012
    Publication date: January 10, 2013
    Inventors: Mark J. Burk, Anthony P. Burgard, Robin E. Osterhout, Jun Sun, Priti Pharkya
  • Publication number: 20120329119
    Abstract: The invention provides non-naturally occurring microbial organisms having a propylene pathway. The invention additionally provides methods of using such organisms to produce propylene.
    Type: Application
    Filed: June 19, 2012
    Publication date: December 27, 2012
    Inventors: Anthony P. Burgard, Robin E. Osterhout, Jun Sun, Priti Pharkya, Mark J. Burk
  • Publication number: 20120252064
    Abstract: The present invention is related to a fungal serine protease enzyme, which said enzyme has serine protease activity and comprises an amino acid sequence of Malbranchea ALKO4122 mature protease as defined in SEQ ID NO:18 or an amino acid sequence having at least 66% identity to the amino acid sequence of SEQ ID NO:18. Also disclosed is an isolated nucleic acid molecule, comprising a polynucleotide sequence which encodes a fungal serine protease enzyme, nucleic acid sequences encoding said protease, a host cell and a process of producing a polypeptide having serine protease activity. Said protease is useful as an enzyme preparation applicable in detergent compositions and for treating fibers, wool, hair, leather, or silk, for treating food or feed, or for any applications involving modification, degradation or removal of proteinaceous material.
    Type: Application
    Filed: March 29, 2012
    Publication date: October 4, 2012
    Applicant: AB ENZYMES OY
    Inventors: Leena Valtakari, Kari Juntunen, Marja Paloheimo, Pentti Ojapalo
  • Publication number: 20120237990
    Abstract: A non-naturally occurring eukaryotic or prokaryotic organism includes one or more gene disruptions occurring in genes encoding enzymes imparting increased fumarate, malate or acrylate production in the organism when the gene disruption reduces an activity of the enzyme. The one or more gene disruptions confers increased production of acrylate onto the organism. Organisms that produce acrylate have an acrylate pathway that at least one exogenous nucleic acid encoding an acrylate pathway enzyme expressed in a sufficient amount to produce acrylate, the acrylate pathway comprising a decarboxylase. Methods of producing fumarate, malate or acrylate include culturing these organisms.
    Type: Application
    Filed: February 13, 2012
    Publication date: September 20, 2012
    Applicant: Genomatica, Inc.
    Inventors: Mark J. BURK, Anthony P. Burgard, Priti Pharkya
  • Publication number: 20120226055
    Abstract: The invention relates to recombinant cells and their use in the production of 3,4-dihydroxybutyrate, 2,3-dihydroxybutyrate and 3-hydroxybutyrolactone.
    Type: Application
    Filed: February 24, 2012
    Publication date: September 6, 2012
    Applicant: Massachusetts Institute of Technology
    Inventors: Himanshu Hemant Dhamankar, Collin Hunter Martin, Kristala Lanett Jones Prather
  • Publication number: 20120213888
    Abstract: The invention relates to a DNA sequence, which codes for a polypeptide having phospholipase activity essentially without lipase activity, characterized in that the DNA sequence is selected from a) DNA sequences that comprise a nucleotide sequence according to SEQ ID NO: 1, b) DNA sequences that comprise the coding sequence according to SEQ ID NO: 1, c) DNA sequences that code for the protein sequence according to SEQ ID NO: 2, d) DNA sequences that are coded for by the plasmid pPL3940-Topo2.5 with the restriction map according to FIG.
    Type: Application
    Filed: October 27, 2010
    Publication date: August 23, 2012
    Applicant: AB ENZYMES GMBH
    Inventors: Khanh Quoc Nguyen, Kornelia Titze, Tatiana Schwarz, Silvia Paladino, Volker Marschner, Patrick Lorenz
  • Publication number: 20120164695
    Abstract: Presently provided are variant glucoamylases displaying altered properties, such as improved thermostability and/or specific activity. Also disclosed are DNA sequences coding for the variants, vectors and host cells incorporating the DNA sequence, enzyme compositions, and methods of using the variants in various applications.
    Type: Application
    Filed: August 18, 2010
    Publication date: June 28, 2012
    Applicant: DANISCO US INC.
    Inventors: Wolfgang Aehle, Richard R. Bott, Martijn Silvan Scheffers, Casper Vroeman
  • Publication number: 20120149087
    Abstract: The present disclosure relates to variants of a parent glucoamylase having altered properties (e.g., improved thermostability and/or specific activity). In particular, the present disclosure provides compositions comprising the variant glucoamylases, including starch hydrolyzing compositions and cleaning compositions. The disclosure also relates to DNA constructs encoding the variants and methods of producing the glucoamylase variants in host cells.
    Type: Application
    Filed: September 30, 2011
    Publication date: June 14, 2012
    Applicant: Danisco US Inc.
    Inventors: Wolfgang Aehle, Richard R. Bott, Martijn Scheffers, Piet Van Solingen, Casper Vroemen
  • Publication number: 20120149064
    Abstract: A filamentous fungal cell is provided comprising at least one mutation, wherein the filamentous fungal cell has impaired ptrB activity and has altered expression of a protein of interest as compared to a corresponding parent filamentous fungal cell. In one embodiment, the altered expression of the protein of interest is enhanced expression of the protein of interest.
    Type: Application
    Filed: November 17, 2009
    Publication date: June 14, 2012
    Applicant: Danisco Us Inc.
    Inventors: Huaming Wang, Guomin Tang, Aoquan Wang, Jinxiang Zhang