Culture Media, Per Se, Or Technique Patents (Class 435/256.8)
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Patent number: 7067303Abstract: Agricultural biomass hydrolysate is detoxified by culturing in the presence of the fungus Coniochaeta ligniaria (teleomorph) or its Lecythophora (anamorph) state. This organism is capable of significantly depleting the toxicant levels of furans, particularly furfural and 5-hydroxymethylfurfural. A new strain of the fungus has been isolated and deposited in the Agricultural Research Culture Collection (NRRL) in Peoria, Ill., as Accession No. NRRL 30616. The detoxified agricultural biomass hydrolysate is useful as a substrate for industrial fermentation processes, especially in saccharification treatments for bioethanol production.Type: GrantFiled: January 24, 2003Date of Patent: June 27, 2006Assignee: The United States of America as represented by the Secretary of AgricultureInventors: Nancy N. Nichols, Maria J. Lopez, Bruce S. Dien, Rodney J. Bothast
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Patent number: 7037711Abstract: The present invention relates to the establishment of a cultivation condition that is suitable for the large-scale production of pharmacologically active filtrates from a culture of A. camphorata, in particular, by optimizing the agitation rate and/or pH value during the cultivation. The present invention also relates to a process for obtaining pharmacologically active compositions from a culture of A. camphorata through a series of fractionation. This invention is further directed to the uses of the above compositions in the preparation of pharmaceutical compositions.Type: GrantFiled: October 30, 2003Date of Patent: May 2, 2006Assignee: Council of AgricultureInventors: Mei-Chiao Wu, Ren-Chang Huang, Shie-Jea Lin, Bor-Cheh Wang
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Patent number: 7018828Abstract: Media and kits are disclosed for use in processes requiring microbial culture. More specifically, the invention provides carrageenan-stabilized agar-based microbial culture media and kits constructed using the media. The media and kits of the invention may allow the construction of kits with increased shelf stability and useful life. Further, the media and kits of the invention may be used in kits and methods useful in the manual determination of the type of infection present in a specimen in periods of about 24 hours. The stabilized culture media of the invention are useful in a broad variety of applications. In an embodiment, the media contains both an agar medium and iota carrageenan.Type: GrantFiled: June 16, 2003Date of Patent: March 28, 2006Inventor: Read Taintor
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Patent number: 6955912Abstract: The present invention is intended to efficiently produce a large amount of chlamydospores of Trichoderma harzianum SK-5-5 mycelia. This objective is achieved by chlamydospores characterized by having been obtained by inoculating a culture medium containing glucose, yeast extract and polypepton with Trichoderma harzianum SK-5-5 mycelia and culturing the same to thereby obtain chlamydospores containing conidiospores.Type: GrantFiled: June 16, 2004Date of Patent: October 18, 2005Assignees: Hokkaido Green Kosan, IncorporatedInventors: Yasuharu Sasaki, Susumu Sasaki
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Patent number: 6902912Abstract: There is disclosed a fermentation process for producing glutathione which comprises (a) the obtainment of a biomass pre-culture by pre-cultivating, in aerobic conditions, a strain of a glutathione producing yeast wherein the glutathione content per biomass unit is higher than 1.2% w/w; (b) the cultivation, in aerobic conditions, of the resulting biomass pre-culture such that the resulting biomass density is higher than 50 g/l; (c) the activation of the cultured biomass; and (d) the recovery of the cultured biomass, extracting glutathione at a pH equal to or lower than 6 and purifying the resulting glutathione. The process allows to obtain glutathione with high yields and relatively low costs.Type: GrantFiled: July 1, 2003Date of Patent: June 7, 2005Assignee: Gnosis SrlInventors: Alberto Benedetti, Enrico Giuseppe Roberto Berardi, Matilde Manzoni, Marina Nichele, Hermes Pagani, Manuela Rollini
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Patent number: 6902925Abstract: The present invention is a process for selective production of beauveriolide I substance or beauveriolide III substance, in which a beauveriolide producing microorganism strain FO-6979 or mutant thereof is cultured in a medium for fungi added with specific amino acid in order to produce beauveriolide I substance or beauveriolide III substance selectively with high yield; beauveriolide I substance or beauveriolide III substance is accumulated selectively in the cultured mass; and beauveriolide I substance or beauveriolide III substance is collected selectively with high yield from said cultured mass.Type: GrantFiled: April 10, 2001Date of Patent: June 7, 2005Assignees: Gakkou Houjin Kitasato Gakuen, Japan Society for the Promotion of ScienceInventors: Satoshi Omura, Hiroshi Tomoda
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Patent number: 6893857Abstract: The invention relates to a method for controlling microorganisms in a sugar-containing aqueous process medium, especially in the sugar industry, using hop bitter acid as active substance. The hop bitter acid is dissolved in an aqueous alkaline medium and added to the process medium. The pH value of the added solution is higher than the pH value of the process medium and the hop bitter acid passes from the dissociated to the indissociated form in the process medium.Type: GrantFiled: February 22, 2000Date of Patent: May 17, 2005Assignees: Beta Tec Hopfenprodukte GmbH, Zuckerforschung Tulln GmbHInventors: John-Paul Maye, David Beddie, Gunter Pollach
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Patent number: 6833266Abstract: A mushroom fungus belonging to the order of Agaricales, is cultured with good efficiency by using a liquid culture medium in such a way as to prepare the mycelia separated from the culture medium such that the culturing liquid can be served for food use. A liquid culture medium containing 3 to 16 g/liter of sucrose and 1 to 6 g/liter of maltose as a carbon source and 0.3 to 1.2 g/liter of yeast extract as a nitrogen source is inoculated with Agaricus mycelia and culturing is conducted by blowing into the culture medium sterilized air of 20 to 90% of the oxygen concentration.Type: GrantFiled: January 25, 2002Date of Patent: December 21, 2004Assignee: Tsukuba Biosystem, Ltd.Inventors: Takaaki Maekawa, Hiroko Isoda
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Patent number: 6812011Abstract: This invention relates to new use of marine cyanobacteria lyngbya, Oscillatoria, Sprulina, Anabaena and Synechocystis deposited with ATCC having accession numbers ATCC PTA-4602 and 4603 for the removal of calcium ions from sea-brine and sub-soil brine having a density range 10 to 25.5° Be′, by culturing the cyanobacteria, inoculating the cyanobacteria culture to raw brine of 10 to 25.5° Be′, filtering the resultant mixture to obtain a brine having less calcium, and to separate the cyanobacteria which can be reused if desired.Type: GrantFiled: February 7, 2001Date of Patent: November 2, 2004Assignee: Council of Scientific & Industrial ResearchInventors: Sandhya Mishra, Pushpito Kumar Ghosh, Mahesh Ramniklal Gandhi, Ajoykumar Murlidhar Bhatt, Shantibhai Amritlal Chauhan
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Patent number: 6803226Abstract: A method of forming an artificial Shiro of Matsutake comprises culturing Matsutake hyphae in a culture substrate containing a substance capable of controlling the cell membrane permeability of the hyphae as an active principle, and a method of forming an artificial Shiro of Matsutake comprises culturing Matsutake hyphae in a culture substrate containing a substance capable of enhancing the hydrophilic property of the hyphae as an active principle.Type: GrantFiled: January 24, 2002Date of Patent: October 12, 2004Assignee: The University of TokyoInventors: Kazuo Suzuki, Alexis Guerin-Laguette, Lu-Min Vaario
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Publication number: 20040157315Abstract: Culture medium composition for fungi and yeast, preparation method and uses of the composition. The culture medium composition is employed for analyzing microorganisms contamination in the food industry, preferably in the cola beverages industry, wherein the composition comprises between 4.5 and 5.5 g/l of monobasic potassium phosphate, between 0.5 and 1.5 g/l of ammonium chloride, between 0.5 and 1.5 g/l of heptahydrate magnesium sulfate, between 30.0 and 50.0 g/l of D(+) saccharose, and water.Type: ApplicationFiled: December 9, 2003Publication date: August 12, 2004Applicant: Coca-Cola Femsa de Buenos Aires S.A.Inventor: Mariana Tajada
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Patent number: 6759232Abstract: Provided is a method for the in vitro aseptic mass production and sporulation of arbuscular mycorrhizal fungi (AMF). The method comprises providing symbiotic root organs with AMF propagules and spores after the root organs are inoculated with AMF inoculums in advance to form AMF propagules in the root organs and cultivating them in an aseptic container, and then providing the whole symbiotic root organs and AMF propagules with liquid medium for a temporary contact between the liquid medium and the root organ with AMF propagules in order to cultivate AMF, then removing the liquid medium from the symbiotic root organs and arbuscular mycorrhizal fungal propagules and finally repeating former two steps periodically to facilitate the mass production and sporulation of the arbuscular mycorrhizal fungi.Type: GrantFiled: April 29, 2002Date of Patent: July 6, 2004Inventor: Wen-Kai Wang
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Publication number: 20040106187Abstract: The present invention relates to a medium composition for the culture of Phellinus linteus strain producing galactomannoglucan and a method for the culture thereof using the same, more particularly, relates to a medium composition for the culture of Phellinus linteus mycelium composed of CSP (corn steep powder), yeast extract, soy peptone, malt extract and distilled water and a method for the culture of Phellinus linteus strain, in which a medium is prepared by autoclaving with steam pressure for 50-60 minutes and cooling, tissues of fruit body or spores of the strain are sub-cultured on agar medium, and then inoculates the sub-cultured products to the medium prepared with the above composition.Type: ApplicationFiled: April 23, 2003Publication date: June 3, 2004Inventors: Nam-Doo Hong, Soo Muk Cho, Jae Kuk Yoo
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Patent number: 6696270Abstract: A cell culture growth substrate comprising submucosal tissue of a warm-blooded vertebrate and a method for culturing fastidious organisms is described. Submucosal tissue used in accordance with the present invention supports the proliferation of cells when said cells are contacted with submucosal tissue under conditions conducive to cell proliferation.Type: GrantFiled: November 14, 2001Date of Patent: February 24, 2004Assignee: Purdue Research FoundationInventors: Stephen F. Badylak, George B. Boder
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Patent number: 6638755Abstract: A simple culture medium produced by impregnating a fibrous water-absorbent sheet with a suspension formed by suspending in an alcohol (a) an adhesive (0.01-0.4 wt. %) which is soluble in both water and alcohol, (b) a gelling agent which is soluble in water and insoluble in alcohol, and (c) a bacterial nutritive ingredient, the fibrous water-absorbent sheet having a mesh larger than the particle size of the gelling agent and being placed on a waterproof flat plate, and by drying the resultant sheet while suppressing rapid evaporation of the alcohol, to thereby cause the water-absorbent sheet to adhere onto the waterproof flat plate; and a method for producing the medium.Type: GrantFiled: November 19, 2001Date of Patent: October 28, 2003Assignee: Nissui Pharmaceutical Co., Ltd.Inventors: Shingo Mizuochi, Hidemasa Kodaka, Hideaki Shibata
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Patent number: 6620609Abstract: The invention relates to a process for a simplified and rapid preparation of sterile culture media using microwave ovens. The sterile media of the invention are usable for the selection and identification of microorganisms transformed by viral or plasmid DNA.Type: GrantFiled: July 14, 1999Date of Patent: September 16, 2003Assignee: CaylaInventors: Elise Armau, GĂ©rard Tiraby
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Publication number: 20030143716Abstract: The present invention is a process for selective production of beauveriolide I substance or beauveriolide III substance, in which a beauveriolide producing microorganism strain FO-6979 or mutant thereof is cultured in a medium for fungi added with specific amino acid in order to produce beauveriolide I substance or beauveriolide III substance selectively with high yield; beauveriolide I substance or beauveriolide III substance is accumulated selectively in the cultured mass; and beauveriolide I substance or beauveriolide III substance is collected selectively with high yield from said cultured mass.Type: ApplicationFiled: January 15, 2003Publication date: July 31, 2003Inventors: Satoshi Omura, Hiroshi Tomoda
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Patent number: 6593127Abstract: The present invention relates to a composition useful for early and profuse sporulation in fungi with about 100 fold increase in spore count within 48 hours of inoculation, said composition comprising molasses ranging between 5-20 g/L, Corn Steep Liquor (CSL) ranging between 10-25 g/L, Sodium chloride (NaCl) ranging between 5-15 g/L, calcium sulphate (CaSO4) ranging between 0.1-0.5 g/L, Potassium dihydrogen phosphate (KH2PO4) ranging between 0.001-0.01 g/L, Magnesium sulphate (MgSO4.7 H2O) ranging between 0.001-0.01 g/L, Copper sulphate (CuSO4) ranging between 0.001-0.0050 g/L, Ferrous sulphate (FeSO4) ranging between 0.0009-0.005 g/L, an anti-foam agent, and optionally solidifying agent and a method thereof.Type: GrantFiled: February 4, 2002Date of Patent: July 15, 2003Assignee: Council of Science and Industrial ResearchInventors: Rajinder Kumar Khajuria, Ram Vilas Parsad Sinha, Vijeshwar Verma, Ghulam Nabi Qazi, Sukhdev Swami Handa
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Patent number: 6576457Abstract: An in vitro system using Agrobacterium-transformed dicotyledon roots (such as carrot roots) and vesicular-arbuscular mycorrhizal fungi (such as Glomus intradices) was developed to produce viable and aseptic spores. The entire life cycle from the growth of initial germ tubes to the formation of external mycelium network and spore production was observed nondestructively using light microscopy. The system may be subcultured continuously for the production of viable and clean spores.Type: GrantFiled: December 15, 2000Date of Patent: June 10, 2003Assignee: The United States of America, as represented by the Secretary of AgricultureInventor: Sui-Sheng T. Hua
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Patent number: 6569475Abstract: Disclosed are a method for culturing mushroom mycelia using grains, a culture product, and use of the culture product. Edible or medicinal mushroom mycelia are inoculated and cultured in solid media made of grains. Induction of the cultured mushroom mycelia to undergo autolysis produces autolysates rich in antitumorigenic and other medicinally useful materials. The squeezing of the autolysates produces a liquid filtrate, leaving a paste. The filtrate is concentrated for use in foods or medicines. The paste is processed into a nutrient-rich gruel or other foods.Type: GrantFiled: October 5, 2001Date of Patent: May 27, 2003Assignees: C.A. Biotech Co., Ltd.Inventors: Jae-Mahn Song, Se-Youn Han, Yun-Sun Na
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Patent number: 6558943Abstract: A solid state fermentation (SSF) method is provided which is effective for both small- and large-scale fungal cultivation. Also provided is SSF media for fungal cultivation. The media preferably contains a carbon source and nitrogen source to provide a carbon to nitrogen ratio of about 5:1 to about 25:1 by weight. The media may also contain a vitamin and an inorganic substance. A preferred SSF medium contains malt extract, yeast extract, peptone, glucose, water, solid base, and calcium carbonate or gypsum. Before propagating fungal mycelia in the SSF medium, the mycelia may be pre-cultivated in a solid culture medium and then in a liquid medium. Although the SSF method can be used in growing most fungi, preferred fungi include Cordyceps sinensis, Ganoderma lucidum, Antrodia camphorata, Trametes versicolor, and Agaricus blazei. The SSF method not only produces high yield of fungi, but also stimulates the production of fungal metabolites, particularly the kinds with pharmaceutical and medicinal activities.Type: GrantFiled: September 5, 2000Date of Patent: May 6, 2003Assignee: Sun Ten Pharmaceutical Co., Ltd.Inventors: Pei-Jung Li, Chung-Guang Shen
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Patent number: 6534305Abstract: A process for aerobic culture in which ammonia and oxygen are simultaneously supplied, comprising: dispersing and supplying air, oxygen, or a mixed gas thereof into a culture tank through a sintered metal membrane attached the end of a diffusing pipe, and simultaneously, dispersing and supplying ammonia or a mixed gas of ammonia and air into the culture tank through a diffusing pipe other than the above diffusing pipe.Type: GrantFiled: December 21, 2001Date of Patent: March 18, 2003Assignee: Ajinomoto Co., Inc.Inventors: Atsushi Okada, Yoshitaka Teratani, Naohiro Kadota, Hisao Itou, Kazuhiro Satou
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Patent number: 6511821Abstract: A process for the preparation of a growth media for mass multiplication of bio-control fungi, comprising a chopped, shade-dried distillation waste in a plastic bag that is plugged with cotton followed by autoclaving and an inoculation of a strain of bio-control fungi such as Trichoderma harzianum or Gliocladium virens, and incubating the bags at room temperature for 14-30 days, and then shade drying and grinding the product to obtain a fine powder.Type: GrantFiled: March 19, 2001Date of Patent: January 28, 2003Assignee: Council of Scientific and Industrial ResearchInventors: Harikesh Bahadur Singh, Alok Kalra, Nirmal Kumar Patra, Sushil Kumar, Rakesh Pandey, Suman Preet Singh Khanuja, Ajit Kumar Shasany
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Publication number: 20020151037Abstract: The present invention is directed to the controlled preparation of mycoculture nutrient substrates and in particular, to methods of composting which include the enzymatic digestion of substrate via colonization with microbial cultures or enzymes. The method of the present invention also include the colonization of substrate with microbial cultures for conferring increased microbial nitrogen content to such substrate. The present invention is further directed to mushroom substrate produced via the methods described herein.Type: ApplicationFiled: February 8, 2001Publication date: October 17, 2002Inventors: Theo van der Sterren, Ger Hendriks, Geert Lemmers, Mark Peter Wach
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Publication number: 20020142451Abstract: The subject invention relates to compounds and compositions which induce transcription of the nolA gene in nitrogen-fixed bacteria, such as Bradyrhizobium japonicum. Novel bacterial strains which are insensitive to NolA, soil inoculants comprising NolA insensitive bacteria and/or nolA inducers, and methods of increasing nitrogen fixation in legumes are also disclosed.Type: ApplicationFiled: March 18, 2002Publication date: October 3, 2002Inventors: John T. Loh, Gary Stacey
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Patent number: 6458580Abstract: A method for promoting the growth of at least one anaerobic fungus in the rumen of a ruminant animal, the method comprising the step of administering to the rumen an effective amount of a degradation resistant sulphur source.Type: GrantFiled: September 27, 2000Date of Patent: October 1, 2002Assignees: Commonwealth Scientific and Industrial Research Organisation, Australian Wool Research and Promotion OrganizationInventors: Geoffrey Leslie Raymond Gordon, Michael William Phillips
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Publication number: 20020094563Abstract: A solid fermentation production method for fungus spores necessitating high nutrient, including steps of mixing grain with yeast, sugar honey, fish milk and water at a certain ratio, steaming/cooking and stirring the mixture to make the grain have high nutrient content, transplanting fungus spores onto the grain after killing bacteria and solidly fermenting the fungus spores to mass-reproduce spores.Type: ApplicationFiled: January 18, 2001Publication date: July 18, 2002Inventors: Suey-Sheng Kao, Chien Yan Hsieh, Yung-Sheng Tsai
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Patent number: 6395271Abstract: The present invention relates a process for culturing isolates of Antrodia camphorata to provide a product useful in medical and nourishment fields. The present invention also relates to a novel isolate of Antrodia camphorata capable of growing in a suitable artificial medium, while exhibiting desired pharmacological activities, in particular anti-tumor activity. The utilization of potato dextrose broth and the synthetic medium containing fructose as major carbon source leads to a significant increase in the pharmacological activity of the cultures of A. camphorate.Type: GrantFiled: October 19, 2000Date of Patent: May 28, 2002Assignee: Council of Agriculture, Executive YuanInventors: Ren-Chang Huang, Jian-Chyi Chen, Bor-Cheh Wang
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Patent number: 6387689Abstract: A method for degrading and/or bioremediating waste wood containing creosote using a fungal inoculum is disclosed. The fungal inoculum comprises of at least one creosote-tolerant fungi, a lignocellulosic substrate and a nutrient supplement. The fungal inoculum is applied to the waste wood and maintained in an aerated and hydrated environment having temperature conditions sufficient to allow the inoculum to grow and metabolize the creosote. The inoculum and the waste wood are combined until an end product is achieved that is at least partially remediated or of a reduced volume.Type: GrantFiled: March 31, 2000Date of Patent: May 14, 2002Assignee: The United States of America as represented by the Secretary of AgricultureInventors: Barbara L. Illman, Vina W. Yang, Leslie A. Ferge
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Patent number: 6383810Abstract: The present invention relates generally to nutritive medium, medium supplement, media subgroup and buffer formulations. Specifically, the present invention provides powder nutritive medium, medium supplement and medium subgroup formulations, particularly cell culture medium supplements (including powdered sera such as powdered fetal bovine serum (FBS)), medium subgroup formulations and cell culture media comprising all of the necessary nutritive factors that facilitate the in vitro cultivation of cells. The invention further provides powder buffer formulations that produce particular ionic and pH conditions upon reconstitution with a solvent.Type: GrantFiled: February 13, 1998Date of Patent: May 7, 2002Assignee: Invitrogen CorporationInventors: Richard Fike, William Whitford, William Biddle
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Patent number: 6379937Abstract: The present invention relates to a novel process for the preparation of mixture of 19 hydroxyeicosatetraenoic acid and 20 hydroxyeicosatetraenoic acid (19 HETE and 20 HETE) by biotransformation of Arachidonic acid or Poly Unsaturated Fatty Acids (PUFA) with yeast as the biotransforming agent.Type: GrantFiled: December 18, 2000Date of Patent: April 30, 2002Assignee: Council of Scientific and Industrial ResearchInventors: Asmita Ashutosh Prabhune, Colin Ratledge
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Patent number: 6372462Abstract: The present invention describes new and distinct strains of higher Basidiomycetes mushrooms, and a process for growing them in submerged culture. Specifically, the new strains of species of the genus Pleurotus offer superior yields of mushroom biomass and concentrations of biologically active compounds, for example, cholesterol-lowering compounds, lectins, proteins, essential amino acids, vitamins or polysaccharides. The process includes use of defined media and a simple one-step procedure of separating the lovastatin-containing nutriceuticals from culture broth.Type: GrantFiled: October 15, 1999Date of Patent: April 16, 2002Assignee: MedMyco Ltd.Inventors: Solomon P. Wasser, Sergey V. Reshetnikov
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Patent number: 6361990Abstract: A method for preparing the high protein nutrient from oilseed-based material is provided. The modified oilseed-based material produced by the process can be utilized in a wide variety of applications, including the preparation of fermentation media. The oilseed-based product typically includes at least about 55 wt. % protein (dry basis), low levels of soluble sugars, such as raffinose, stachyose and saccharose and has a FAN content of at least about 15 mg/g. Fermentation media and aqueous nutrient solutions derived from the modified oilseed material are also provided.Type: GrantFiled: September 27, 2000Date of Patent: March 26, 2002Assignee: Cargill, Inc.Inventors: Michael A. Porter, Alison M. Jones, Dean A. Ducan, Michael D. Kluetz
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Patent number: 6355475Abstract: The present invention relates a process for culturing isolates of Antrodia camphorata to provide a product useful in medical and nourishment fields. The present invention also relates to a novel isolate of Antrodia camphorata capable of growing in a suitable artificial medium, while exhibiting desired pharmacological activities, in particular anti-tumor activity. The utilization of potato dextrose broth and the synthetic medium containing fructose as major carbon source leads to a significant increase in the pharmacological activity of the cultures of A. camphorata.Type: GrantFiled: October 19, 2000Date of Patent: March 12, 2002Assignee: Council of Agriculture, Executive YuanInventors: Ren-Chang Huang, Jian-Chyi Chen, Bor-Cheh Wang
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Publication number: 20020028500Abstract: A process for the preparation of a growth media for mass multiplication of bio-control fungi, comprising a chopped, shade-dried distillation waste in a plastic bag that is plugged with cotton followed by autoclaving and an inoculation of a strain of bio-control fungi such as Trichoderma harzianum or Gliocladium virens, and incubating the bags at room temperature for 14-30 days, and then shade drying and grinding the product to obtain a fine powder.Type: ApplicationFiled: March 19, 2001Publication date: March 7, 2002Applicant: Council of Scientific and Industrial ResearchInventors: Harikesh Bahadur Singh, Alok Kalra, Nirmal Kumar Patra, Sushil Kumar, Rakesh Pandey, Suman Preet Singh Khanuja, Ajit Kumar Shasany
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Patent number: 6340586Abstract: The strain of the microorganism Penicillium oxalicum var. Armeniaca CCM 8242 produces an anthraquinonyl carboxylic acid derivative of structural formula (I), which may be used as a colorant, especially as a food colorant or cosmetic colorant.Type: GrantFiled: September 22, 2000Date of Patent: January 22, 2002Inventor: Eduard Sardaryan
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Patent number: 6335196Abstract: This invention is concerned with determining the three-dimensional structure of biological macromolecules such as proteins. In particular, it is concerned with methods for rapidly determining protein structure by NMR, by providing methods for simplifying NMR spectra using labeled proteins prepared from specifically isotopically labeled amino acids having at least two isotopes of 13C, 15N and 2H in the backbone, and methods for making these labeled proteins, e.g., by cultivation of a microbial culture containing said labeled amino acids.Type: GrantFiled: March 15, 2000Date of Patent: January 1, 2002Assignee: Martek Biosciences Corp.Inventors: Frank Elbert Anderson, III, Jonathan Miles Brown, Philip Edward Coughlin, Steven William Homans, Charles Tobias Weller, Carrie Ann Zimmerman-Wilson, Eddie James Oliver, Jr.
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Patent number: 6265203Abstract: A culture material is formed containing a water-soluble polymer layer and a porous matrix layer arranged such that when water is applied to the surface of the matrix layer the water diffuses through the matrix layer to dissolve the water-soluble polymer layer which then gradually penetrates into the matrix layer to form a single layer for use as a culture medium on which a microorganism can be grown to form colonies. The water-soluble polymer layer or water added to the matrix layer can contain nutrients for the microorganism, and the microorganism can be added with the water. The culture material has a water content of not more than 50%, and is sufficiently insoluble in water such that it substantially holds its original shape for at least 30 minutes when in contact with water having a temperature of not more than 50° C.Type: GrantFiled: June 29, 1998Date of Patent: July 24, 2001Assignee: Chisso CorporationInventor: Masashi Ushiyama
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Patent number: 6197571Abstract: The present invention relates to preparation of protein polysaccharide 0041 obtained by extracting a dried product of cultured mycelia of Agaricus blazei Murrill 0041 with hot water, and protein polysaccharide 0041 shows immunopotentiating antitumor activity when administered, so that it is useful as an antitumor agent against various tumors, and as an immunopotentiating substance against other diseases.Type: GrantFiled: December 3, 1998Date of Patent: March 6, 2001Assignee: Agaricus Laboratories Co., Ltd.Inventors: Manabu Hikichi, Shigenobu Okubo, Eiji Hiroe