Proteinaceous Material Recovered Or Purified Patents (Class 435/272)
  • Patent number: 10370651
    Abstract: The invention provides methods and compositions for identifying binding polypeptides (e.g., antibodies or antigen binding fragments thereof) that specifically binds to a cell-surface antigen. The methods of the invention generally comprise contacting a variegated nucleic acid-display library of binding polypeptides with a cell-surface antigen displayed on the exterior surface of a cell; and isolating from the library at least one library member that specifically binds to the cell-surface antigen on the exterior surface of the cell.
    Type: Grant
    Filed: June 20, 2014
    Date of Patent: August 6, 2019
    Assignee: X-BODY, INC.
    Inventors: Yan Chen, Steven M. Shamah
  • Patent number: 10323264
    Abstract: The invention provides a method for producing methacrylyl-CoA that converts 3-hydroxyisobutyryl-CoA into methacrylyl-CoA using an enzyme having dehydratase activity as a method for producing methacrylyl-CoA using an enzyme catalyst. In this production method, conversion rate of 3-hydroxyisobutyryl-CoA into methacrylyl-CoA by the enzyme having dehydratase activity is 50% or higher. In this production method, furthermore, the enzyme having dehydratase activity derives from a microorganism belonging to the genus Pseudomonas or Rhodococcus.
    Type: Grant
    Filed: July 25, 2014
    Date of Patent: June 18, 2019
    Assignee: Mitsubishi Chemical Corporation
    Inventors: Eiji Sato, Fujio Yu, Eiji Nakajima, Michiko Yamazaki, Wataru Mizunashi
  • Patent number: 9688762
    Abstract: The present inventors succeeded in improving the antibody constant region to have increased stability under acid conditions, reduced heterogeneity originated from disulfide bonds in the hinge region, reduced heterogeneity originated from the H chain C terminus, and increased stability at high concentrations as well as in discovering novel constant region sequences having reduced Fc? receptor-binding, while minimizing the generation of novel T-cell epitope peptides. As a result, the present inventors successfully discovered antibody constant regions with improved physicochemical properties (stability and homogeneity), immunogenicity, safety, and pharmacokinetics.
    Type: Grant
    Filed: September 26, 2008
    Date of Patent: June 27, 2017
    Assignee: Chugai Sciyaku Kabushiki Kaisha
    Inventors: Tomoyuki Igawa, Hirotake Shiraiwa
  • Patent number: 9080167
    Abstract: Method and apparatus for processing paraffin embedded samples, e.g., to disassociate paraffin from tissue components and/or other biomolecules from the paraffin. The sample may be exposed to focused acoustic energy while held in a vessel containing a non-solvent, aqueous solution. Disassociated paraffin may be emulsified into the liquid or otherwise separated from the sample.
    Type: Grant
    Filed: November 16, 2012
    Date of Patent: July 14, 2015
    Assignee: Covaris, Inc.
    Inventors: James A. Laugharn, Jr., Edwin Rudd, Guillaume Durin
  • Publication number: 20150140644
    Abstract: The present disclosure relates to methods of increasing the purity of an apoplast-targeted recombinant protein recovered from a plant tissue, and increasing the yield of recovery of an apoplast-targeted recombinant protein from a plant tissue.
    Type: Application
    Filed: November 18, 2014
    Publication date: May 21, 2015
    Inventors: Karen A. MCDONALD, Nathaniel J. KINGSBURY
  • Publication number: 20150132834
    Abstract: Disclosed is a method for selectively eliminating triglycerides in lipoproteins other than low density lipoprotein, which method allows one to provide a method for directly and differentially quantifying LDL-TG in a sample with excellent simplicity, specificity and accuracy using an automated analyzer or the like without performing a laborious operation of pretreatment such as centrifugation or electrophoresis. The method for eliminating triglycerides in lipoproteins other than low density lipoproteins includes allowing lipoprotein lipase, cholesterol esterase, glycerol kinase and glycerol-3-phosphate oxidase to act on a sample in the presence of a surfactant that acts on lipoproteins other than low density lipoprotein and/or a surfactant having LDL-protecting action, and eliminating hydrogen peroxide produced thereby.
    Type: Application
    Filed: April 19, 2013
    Publication date: May 14, 2015
    Applicant: DENKA SEIKEN CO., LTD.
    Inventor: Motoko Ohta
  • Publication number: 20150118730
    Abstract: The present invention is directed to enzyme based methods for separating protein from protein-rich materials derived from plant seeds, fruit, or other biomass and products made therefrom. The protein content in the resulting products is improved by separating and removing the carbohydrates from around the proteins in, for example, soybean meal. This removal is facilitated by the enzymatic hydrolysis of poly- and oligomeric carbohydrates into monosaccharides and other water soluble sugars. The present invention provides for the production of three streams of useful materials. The first is an enriched protein material comparable to the known SPCs but without significant quantities of undigestible oligosaccharides and polysaccharides. The second is an SPI made from the soluble protein in the hydrolysate which is valuable for high-quality feed, food and industrial uses.
    Type: Application
    Filed: May 9, 2013
    Publication date: April 30, 2015
    Applicant: THE UNIVERSITY OF AKRON
    Inventors: Lu-Kwang Ju, Abdullah Loman, Anthony Coffman, Qian Li, Srujana Koganti
  • Publication number: 20150010988
    Abstract: A method for removing protein from a food includes adding a first group of enzymes into a food while cooking the food. The first group of enzymes decomposes protein in the food in a first enzyme reaction. Let a mixture of the first group of enzymes and the food rest after cooling the mixture to a temperature below 45° C. Then, the mixture is boiled to terminate the first enzyme reaction. A second enzyme is added into the mixture after the boiled mixture is cooled to a temperature below 45° C., and let mixture of the first group of enzymes, the food, and the second enzyme rest. The second enzyme carries out a second-stage decomposition of protein in the food in a second enzyme reaction. Then, the mixture of the first group of enzymes, the food, and the second enzyme is boiled to terminate the second enzyme reaction.
    Type: Application
    Filed: July 3, 2013
    Publication date: January 8, 2015
    Inventor: Liang Ting Lu
  • Publication number: 20140302593
    Abstract: Processes and compositions to produce, package, and purify virus like particles containing heterologous cargo molecules utilizing self assembling proteins and protease treatment coupled with simple precipitation and filtration methods are described.
    Type: Application
    Filed: May 16, 2014
    Publication date: October 9, 2014
    Applicant: APSE, LLC
    Inventors: Juan Pedro Humberto Arhancet, Juan P. Arhancet, Kimberly Delaney, Kathleen B. Hall, Neena Summers
  • Patent number: 8828709
    Abstract: The invention relates to a method with which proteins from formalin-fixed biological samples can be dissolved and subsequently quantified. The method makes it possible to extract intact full-length proteins from the samples and to conduct a subsequent analysis thereof.
    Type: Grant
    Filed: May 10, 2006
    Date of Patent: September 9, 2014
    Assignee: Qiagen GmbH
    Inventors: Peter Porschewski, Karl-Friedrich Becker
  • Publication number: 20140212952
    Abstract: Expression vector systems are provided for increased production of a recombinant GDF-5 (rhGDF-5) protein. Also provided are transformed host cells that were engineered to produce and express high levels of rhGDF-5 protein. Methods for isolating recombinant GDF-5 protein from an inclusion body of a cell are disclosed herein. The methods as disclosed are cost-effective, time-saving and are of manufacturing quality.
    Type: Application
    Filed: January 25, 2013
    Publication date: July 31, 2014
    Applicant: Warsaw Orthopedic, Inc.
    Inventors: David S. Scher, Roger E. Harrington
  • Patent number: 8758829
    Abstract: The present invention relates to a process for removing fluorine from krill material by subjecting the krill to disintegration and to an enzymatic hydrolysis process prior to or simultaneously with a removal of the exoskeleton particles producing a fluorine-reduced product. The process of the invention can process krill material with a high polar lipid content for producing superior quality, low fluorine, products suitable for the food and feed as well as the pharmaceutical, nutraceutical and cosmetic industry.
    Type: Grant
    Filed: September 14, 2009
    Date of Patent: June 24, 2014
    Assignee: Emerald Fisheries AS
    Inventors: Stig Tore Kragh Jansson, Jon Reidar Ervik, Leif Grimsmo
  • Publication number: 20140135235
    Abstract: Highly specific and novel methods for reversible hydrazone solid-phase extraction (rHSPE) are provided for glycan or glycopeptide isolation from proteins, peptides, and other contaminants for glycan and glycopeptide analysis. Glycans or glycopeptides in complex mixtures can be conjugated onto solid support or affinity or chemical tags via reversible hydrazone bond. The conjugation methods of the present invention are chemically specific and provide unique means for the removal of other non-glycan containing molecules in the complex sample before the glycans or glycopeptides are hydrolyzed and recovered for analysis. The hydrazone formation and hydrolysis of the novel methods allows for the analysis of glycans and glycopeptides. The hydrazide coating on the solid-phase surfaces are useful for surface glycan capture and on target glycan analysis. Uses of the information generated by the inventive methods for diagnosis and treatment are also disclosed.
    Type: Application
    Filed: June 6, 2012
    Publication date: May 15, 2014
    Inventors: Hui Zhang, Shuang Yang
  • Patent number: 8722337
    Abstract: The instant invention provides an intergrated kit with solutions and instruments to isolate various biological samples from biological samples. The kit enables an user to preserve specimens and isolate biomolecules with features and benefits of high quality, easy, fast, no toxicity, safe to user and environment, low demanding, cost-effective, reducing waste, saving nature resources and protecting environment, and leads to a low-carbon and Green economy in preparation of specimens. The integrated kit enables the user to extract biomolecules including DNA/ccfDNA, Large RNA/mRNA/ccfRNA, Small RNA/miRNA/ccfmiRNA, Protein, Lipid, Carbohydrates, and Metabolite using one seamless procedure.
    Type: Grant
    Filed: August 6, 2013
    Date of Patent: May 13, 2014
    Assignee: BexMart
    Inventors: Bob Han, Eric Han, Xiaohui Xiong, Xiaoliang Han
  • Publication number: 20140065128
    Abstract: The invention provides an EndoS polypeptide, or a polynucleotide encoding an EndoS polypeptide, for use in a method for treating or preventing a disease or condition mediated by IgE antibodies.
    Type: Application
    Filed: March 5, 2012
    Publication date: March 6, 2014
    Applicant: HANSA MEDICAL AB
    Inventors: Mattias Collin, Rolf Lood, Karl Carlstrom, Maria Allhorn, Jonathan Sjogren, Falk Nimmerjahn
  • Publication number: 20140057999
    Abstract: (Modified) rice endosperm protein is used as novel protective hydrocolloid for active ingredients, especially fat-soluble active ingredients and/or colorants. Included are compositions comprising (modified) rice endosperm protein and at least one active ingredient and to their manufacture, as well as to the (modified) rice endosperm protein itself and its manufacture. These compositions are used for the enrichment, fortification and/or coloration of food, beverages, animal feed, personal care or pharmaceutical compositions, and to food, beverages, animal feed, personal care and pharmaceutical compositions containing such a (modified) rice endosperm protein and such a composition, respectively.
    Type: Application
    Filed: February 8, 2013
    Publication date: February 27, 2014
    Applicant: DSM IP ASSETS B.V.
    Inventor: DSM IP ASSETS B.V.
  • Publication number: 20140047571
    Abstract: The present invention relates to polypeptides having phytase activity. These polypeptides have an amino acid sequence which has at least 70% identity to either of three phytases derived from the bacterium Buttiauxella, and which comprises at least one of the following amino acids at the position indicated: 119N, 120L, and/or 121E. These phytases have an improved specific activity. Additional specific amino acid substitutions are also disclosed which characterize and distinguish additional phytases of the invention having improved properties such as temperature and/or pH stability, pH activity profile, temperature activity profile, substrate profile, improved performance in animal feed in vitro or in vivo. The invention also relates to isolated polynucleotides encoding the polypeptides, nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.
    Type: Application
    Filed: October 11, 2013
    Publication date: February 13, 2014
    Applicant: Novozymes A/S
    Inventors: Carsten Sjoeholm, Soeren Flensted Lassen, Lars Kobberoee Skov, Leonardo De Maria
  • Publication number: 20140024104
    Abstract: Methods of recovering plant-derived proteins or suprastructure proteins, are provided. The method may comprise obtaining a plant, or plant matter comprising apoplast-localized proteins, or suprastructure proteins, loosening the cell wall to produce a plant or plant matter having a loosened cell wall, thus allowing the release of apoplastic content through the cell wall to produce an apoplastic content fraction from the plant or plant matter, and recovering the apoplastic content fraction. The apoplastic content fraction comprises plant-derived proteins or suprastructure proteins.
    Type: Application
    Filed: March 23, 2012
    Publication date: January 23, 2014
    Applicant: MEDICAGO INC.
    Inventors: Manon Couture, Dany Paquet, Louis-Philippe Vezina
  • Patent number: 8628690
    Abstract: A nanoemulsion composition having enhanced oxidative stability, emulsion stability, and health benefits. The composition may include individual ingredients or a synergistic blend of non-reducing sugars, sugar polyols, medium-chain triglycerides, polysaccharides, polyphenols, phospholipids, chitosan, and alpha-casein, beta-casein, kappa-casein or protein fragments, glycopeptides, phosphopeptides. The composition may optionally be further utilized for the prevention of hypercholesterolemia or bone (and teeth) mineral loss.
    Type: Grant
    Filed: January 3, 2006
    Date of Patent: January 14, 2014
    Assignee: The Texas A&M University System
    Inventors: Adela Mora-Gutierrez, Michael H Gurin
  • Publication number: 20130344576
    Abstract: The technology disclosed herein relates to novel methods and compositions for the production processes of algae cell components from algae of the genus Scenedesmus. The extractability of algae cellular components is improved and the process is optimized and accelerated by using the enzyme compositions according to the present invention.
    Type: Application
    Filed: January 2, 2012
    Publication date: December 26, 2013
    Applicant: DIREVO INDUSTRIAL BIOTECHNOLOGY GmbH
    Inventors: Klaudija Milos, Claudia Stamme, Tanja Lindner, Vitaly Svetlichny, Andreas Scheidig
  • Publication number: 20130337528
    Abstract: This invention relates generally to the fields of separation and conversion technologies, and more particularly to materials for use in tangential-flow filtration techniques. The tangential-flow materials are useful in a wide range of separation and conversion processes, including those reliant on reverse osmosis, microfiltration, ultrafiltration, or nanofiltration semipermeable filtration membranes, and provide efficient methods for purifying or producing various target substances, including biopolymer particles for use in tangential-flow filtration.
    Type: Application
    Filed: December 9, 2011
    Publication date: December 19, 2013
    Inventors: Tracy Thompson, Bernd Helmut Rehm, Andrew Brian Herbert, Edward George Saravolac
  • Patent number: 8580558
    Abstract: This invention provides methods of isolating ferritin from plant and animal material. The isolated ferritin can be administered to humans or animals in need of iron, and can be used to treat or supplement iron deficiency. The isolated ferritin can be used in industrial applications, such as increasing the iron content in heat-processed food or beverages. The methods of the invention also include quantitation of iron derived from plant or animal ferritin.
    Type: Grant
    Filed: June 3, 2013
    Date of Patent: November 12, 2013
    Assignee: Slo-Iron, LLC
    Inventor: Elizabeth C. Theil
  • Patent number: 8574883
    Abstract: A method of co-extracting multiple proteins from chicken egg white is provided. Precipitate proteins step-by-step with polyethylene glycol, then separate the proteins via Q Sepharose Fast Flow anion-exchange chromatography. The method of co-extracting multiple proteins from chicken egg white is capable of obtaining five proteins simultaneously in one extraction process, and the protein products have high purity and high recovery ratio. And the method can be carried out only requiring common-used chromatographic filler, greatly reduces production cost, and provides favorable factors for large-scale industrial production of extracting protein from egg white.
    Type: Grant
    Filed: January 18, 2013
    Date of Patent: November 5, 2013
    Assignee: Huazhong Agricultural University
    Inventors: Meihu Ma, Fang Geng, Qun Huang
  • Publication number: 20130267013
    Abstract: This invention provides methods of isolating ferritin from plant and animal material. The isolated ferritin can be administered to humans or animals in need of iron, and can be used to treat or supplement iron deficiency. The isolated ferritin can be used in industrial applications, such as increasing the iron content in heat-processed food or beverages. The methods of the invention also include quantitation of iron derived from plant or animal ferritin.
    Type: Application
    Filed: June 3, 2013
    Publication date: October 10, 2013
    Inventor: Elizabeth C. Theil
  • Patent number: 8545911
    Abstract: A phorbol ester is decomposed by mixing an organic substance containing a phorbol ester and Bacillus substilis var. natto and subjecting the resulting mixture to fermentation. At this time, 4 parts by mass of organic substance containing a phorbol ester is mixed with 0.5 to 3 parts by mass of water, and the resulting mixture is subjected to high-temperature and high-pressure sterilization. Then, a solution obtained by dissolving 0.004 to 0.2 part by mass of Bacillus subtilis var. natto in 0.5 to 1 part by mass of water is added, and the resulting mixture is subjected to fermentation at 30 to 50° C. for two to four weeks.
    Type: Grant
    Filed: February 9, 2010
    Date of Patent: October 1, 2013
    Assignees: Idemitsu Kosan Co., Ltd., Nippon Biodiesel Fuel Co., Ltd.
    Inventors: Yuxi He, Hideo Kikutsugi
  • Patent number: 8530228
    Abstract: The invention of Integrated Versatile and Systems Preparation of Specimens relates an open module technology which integrates synchronously the methods of protection, isolation and alteration of specimens into the “One for All” product or kit. The product or kit comprises a core module without or with Plug-in modules and a set of comprehensive protocols which can simultaneously or individually isolate systems biomolecules including DNA/ccfDNA, Large RNA/mRNA/ccfRNA, Small RNA/miRNA/ccfmiRNA, Protein, Lipid, Carbohydrates, and Metabolite versatilely from a vast variety of specimens including solid specimens and liquid specimens. The product or kit can accept new and custom Plug-in modules to expand functions and applications.
    Type: Grant
    Filed: December 28, 2010
    Date of Patent: September 10, 2013
    Assignee: Bexmart
    Inventors: Bob Han, Eric Han, Xiaohui Xiong, Xiaoliang Han
  • Publication number: 20130225790
    Abstract: A Momordica charantia polypeptide, preparation method thereof and uses of the same are provided. This polypeptide is prepared according to the following steps: the bitter gourd powder is enzymolysed with papain to give a polypeptide with a molecular weight of 130˜1000 Da. The resultant polypeptide consists of 2-9 amino acids, is easily absorbed by human body, and possesses the biological function of balancing blood glucose. The Momordica charantia polypeptide provided in the present application may be used for preparation of a medicine, food or food additives for the prophylaxis and/or treatment of diabetes.
    Type: Application
    Filed: October 21, 2011
    Publication date: August 29, 2013
    Inventor: Yuandong Zou
  • Patent number: 8507246
    Abstract: The formation of acrylamide during heat treatment in the production of a food product is reduced by treating the raw material with an enzyme before the heat treatment. The enzyme is capable of reacting on asparagine or glutamine (optionally substituted) as a substrate or is a laccase or a peroxidase.
    Type: Grant
    Filed: January 18, 2012
    Date of Patent: August 13, 2013
    Assignee: Novozymes A/S
    Inventors: Gitte Budolfsen, Morten Tovborg Jensen, Hans Peter Heldt-Hansen, Mary Ann Stringer
  • Publication number: 20130195829
    Abstract: The present invention relates to a method for producing phytase variants which has at least 74% identity to a phytase derived from Citrobacter braakii and comprises at least two additional disulfide bonds as compared to this phytase. These phytase variants have modified, preferably improved, properties, such as thermostability, temperature profile, pH profile, specific activity, performance in animal feed, reduced protease sensitiliby, and/or an modified glycosylation pattern. The invention also relates to the variants produced, DNA encoding these phytases, methods of their production, as well as the use thereof, e.g. in animal feed and animal feed additives.
    Type: Application
    Filed: March 25, 2011
    Publication date: August 1, 2013
    Applicant: NOVOZYMES A/S
    Inventors: Lars Koberoee Skov, Leonardo De Maria, Tomoko Matsui
  • Publication number: 20130171658
    Abstract: The invention provides more rapid and cost-effective methods of deglycosylating target glycoproteins. In methods of the invention, the target glycoprotein is isolated from initial samples, which may contain multiple other glycoproteins, by subjecting the initial sample to a solid phase containing an affinity ligand, such as a deglycosylated antibody, that interacts specifically with the target glycoprotein. Once separated from the sample, the target glycoprotein can be deglycosylated in situ, or eluted from the solid phase, quantitated, and then deglycosylated.
    Type: Application
    Filed: September 15, 2011
    Publication date: July 4, 2013
    Applicant: PROZYME, INC.
    Inventors: Scott P. Fulton, Steven M. Murphy
  • Patent number: 8476061
    Abstract: This invention provides methods of isolating ferritin from plant and animal material. The isolated ferritin can be administered to humans or animals in need of iron, and can be used to treat or supplement iron deficiency. The isolated ferritin can be used in industrial applications, such as increasing the iron content in heat-processed food or beverages. The methods of the invention also include quantitation of iron derived from plant or animal ferritin.
    Type: Grant
    Filed: December 28, 2012
    Date of Patent: July 2, 2013
    Assignee: Slo-Iron, LLC
    Inventor: Elizabeth C. Theil
  • Publication number: 20130164827
    Abstract: The present invention provides methods for separating proteins from a protein mixture. In one aspect, a method for separating a high concentration protein mixture into a bound protein fraction and a flow-through protein fraction can include delivering a protein mixture through an ion exchange column at a fixed pH and a fixed salt concentration. The fixed pH and the fixed salt concentration have been preselected to cause separation of the protein mixture into a bound protein fraction and a flow-through protein fraction. In this case, the bound protein fraction binds to the ion exchange column and the flow-though protein fraction flows though the ion exchange column. The method can further include receiving the flow-through protein fraction from the ion exchange column separate from the bound protein fraction, wherein either the bound protein fraction or the flow-through fraction contains a protein of interest.
    Type: Application
    Filed: November 5, 2012
    Publication date: June 27, 2013
    Inventor: Anthony R. Torres
  • Publication number: 20130067807
    Abstract: Methods of preparing plant-derived proteins or suprastructure proteins, are provided. The method may comprise obtaining a plant, or plant matter comprising apoplast-localized proteins, or suprastructure proteins, producing a protoplast/spheroplast fraction and apoplast fraction from the plant or plant matter, and recovering the apoplast fraction. The apoplast fraction comprises plant-derived proteins or suprastructure proteins. Alternatively, the proteins, or suprastructure proteins, may be obtained from plant or plant matter comprising plant-derived proteins or suprastructure proteins, by digesting the plant matter using a cell wall degrading enzyme composition to produced a digested fraction. The digested fraction is filtered to produced a filtered fraction, and the plant-derived proteins or suprastructure proteins, are recovered from the filtered fraction.
    Type: Application
    Filed: September 21, 2010
    Publication date: March 21, 2013
    Applicant: MEDICAGO INC.
    Inventors: Louis-Philippe Vezina, Manon Couture, Dany Paquet, Michele Dargis, Marc-Andre D'Aoust
  • Publication number: 20130040342
    Abstract: The present invention relates to a method for producing phytase variants has at least 70% identity to a phytase derived from Buttiauxella and comprises at least one additional disulfide bond as compared to this phytase. These phytase variants have modified, preferably improved, properties, such as thermostability, temperature profile, pH profile, specific activity, performance in animal feed, reduced protease sensitiliby, and/or an modified glycosylation pattern. The invention also relates to the variants produced, DNA encoding these phytases, methods of their production, as well as the use thereof, e.g. in animal feed and animal feed additives.
    Type: Application
    Filed: March 25, 2011
    Publication date: February 14, 2013
    Applicant: NOVOZYMES A/S
    Inventors: Leonardo De Maria, Lars Kobberoee Skov, Michael Skjoet
  • Patent number: 8357408
    Abstract: Proteases derived from Nocardiopsis dassonvillei subsp. dassonvillei DSM 43235, Nocardiopsis prasina DSM 15649, Nocardiopsis prasina (previously alba) DSM 14010 Nocardiopsis sp. DSM 16424, Nocardiopsis alkaliphila DSM 44657 and Nocardiopsis lucentensis DSM 44048, as well as homologous proteases; their recombinant production in various hosts, including transgenic plants and non-human animals, and their use in animal feed and detergents. The proteases are acid-stable, alkali-stable, and/or thermostable.
    Type: Grant
    Filed: June 17, 2005
    Date of Patent: January 22, 2013
    Assignee: Novozymes A/S
    Inventors: Soren Flensted Lassen, Carsten Sjoholm, Peter Rahbek Ostergaard, Morten Fischer
  • Publication number: 20130017185
    Abstract: The present invention relates to a phytase which has at least 70% identity to a phytase derived from E. coli and comprises at least one modification as compared to this phytase. These phytase variants have modified, preferably improved, properties, such as thermostability, temperature profile, pH profile, specific activity, performance in animal feed, reduced protease sensibility, and/or an modified glycosylation pattern. The invention also relates to DNA encoding these phytases, methods of their production, as well as the use thereof, e.g. in animal feed and animal feed additives.
    Type: Application
    Filed: March 25, 2011
    Publication date: January 17, 2013
    Applicant: NOVOZYMES A/S
    Inventors: Leonardo De Maria, Lars Kobberoee Skov, Michael Skjoet
  • Publication number: 20120329993
    Abstract: A process for recovering and isolating soy whey proteins and other components from whey processing streams is disclosed.
    Type: Application
    Filed: December 30, 2010
    Publication date: December 27, 2012
    Applicant: LEGAL DEPARTMENT
    Inventors: Darrell Marsh, Karsten Keller, Charles S. Schasteen, Barry Tulk, Marlene Mekel, Jennifer Wu
  • Publication number: 20120321747
    Abstract: Proteases derived from Nocardiopsis dassonvillei subsp. dassonvillei DSM 43235, Nocardiopsis prasina DSM 15649, Nocardiopsis prasina (previously alba) DSM 14010 Nocardiopsis sp. DSM 16424, Nocardiopsis alkaliphila DSM 44657 and Nocardiopsis lucentensis DSM 44048, as well as homologous proteases; their recombinant production in various hosts, including transgenic plants and non-human animals, and their use in animal feed and detergents. The proteases are acid-stable, alkali-stable, and/or thermostable.
    Type: Application
    Filed: June 7, 2012
    Publication date: December 20, 2012
    Applicant: NOVOZYMES A/S
    Inventors: Soren Flensted Lassen, Carsten Sjoeholm, Peter Rahbek Oestergaard, Morten Fischer
  • Publication number: 20120321746
    Abstract: Proteases derived from Nocardiopsis dassonvillei subsp. dassonvillei DSM 43235, Nocardiopsis prasina DSM 15649, Nocardiopsis prasina (previously alba) DSM 14010 Nocardiopsis sp. DSM 16424, Nocardiopsis alkaliphila DSM 44657 and Nocardiopsis lucentensis DSM 44048, as well as homologous proteases; their recombinant production in various hosts, including transgenic plants and non-human animals, and their use in animal feed and detergents. The proteases are acid-stable, alkali-stable, and/or thermostable.
    Type: Application
    Filed: June 7, 2012
    Publication date: December 20, 2012
    Applicant: NOVOZYMES A/S
    Inventors: Soren Flensted Lassen, Carsten Sjoeholm, Peter Rahbek Ostergaard, Morten Fischer
  • Publication number: 20120301894
    Abstract: Provided in certain embodiments are new methods for forming azido modified biomolecule conjugates of reporter molecules, carrier molecules or solid support. In other embodiments are provided methods for enzymatically labeling a biomolecules with an azide group.
    Type: Application
    Filed: February 17, 2012
    Publication date: November 29, 2012
    Applicant: Life Technologies Corporation
    Inventors: Brian AGNEW, Kyle R. Gee, Tamara G. Nyberg
  • Publication number: 20120276075
    Abstract: The present invention relates to a novel enzyme composition comprising a prolyl protease and tripeptidyl proteases having unique catalytic properties. The present invention further relates to methods for producing the enzyme composition as well as a pharmaceutical composition and a food supplement containing the enzyme composition and its use in the degradation of polypeptides.
    Type: Application
    Filed: December 20, 2010
    Publication date: November 1, 2012
    Applicant: CENTRE HOSPITALIER UNIVERSITAIRE VAUDOIS (CHUV)
    Inventors: Michel Monod, Eric Grouzmann
  • Publication number: 20120264199
    Abstract: The present invention is related to a preparation method of the compounds for absorbing heavy metals. First of all, prepare natural vegetable or fruit and extract a B-M sulfur peptide from the said natural vegetable or fruit. Second, place the natural vegetable or fruit into fermentation and extract the mixed nutrient extraction. And then mix the B-M sulfur peptide and the mixed nutrient extraction as the final extraction. Mix the final extraction into drinks. The final extraction will slowly release B-M sulfur peptide when entering the body; through its combination of heavy metals and re-absorption by the body, useful materials are retained in the human body while long-term accumulated toxic metals are discharged to repair the body's metabolism.
    Type: Application
    Filed: April 18, 2011
    Publication date: October 18, 2012
    Inventor: Yung-Cheng Lin
  • Publication number: 20120252065
    Abstract: The present disclosure relates to an aqueous process for the preparation of a protein isolate and a hydrolyzed protein concentrate from an oilseed meal, optionally comprising: mixing an oilseed meal with an aqueous solvent to form a slurry; optionally treating the slurry with phytase y; separating the slurry with a solid/liquid separation to form: a liquid phase, comprising the aqueous solvent, soluble protein and oil; and a solid phase comprising insoluble protein; separating the liquid phase to form: an oil phase; and an aqueous protein phase; subjecting the aqueous protein phase to membrane filtration to obtain a protein solution; and drying the protein solution to obtain the protein isolate subjecting the insoluble protein to enzymatic hydrolysis, and subjecting the hydrolyzed protein to membrane filtration to obtain an amino acid and peptide solution; and drying the amino acid and peptide solution to obtain the hydrolyzed protein concentrate.
    Type: Application
    Filed: April 3, 2012
    Publication date: October 4, 2012
    Inventors: Luis Rozenszain, Garrison Beye
  • Publication number: 20120244594
    Abstract: The invention provides methods for isolating a modified peptide from a complex mixture of peptides, the method comprising the steps of: (a) obtaining a proteinaceous preparation from an organism, wherein the preparation comprises modified peptides from two or more different proteins; (b) contacting the preparation with at least one immobilized modification-specific antibody; and (c) isolating at least one modified peptide specifically bound by the immobilized modification-specific antibody in step (b). The method may further comprise the step of (d) characterizing the modified peptide isolated in step (c) by mass spectrometry (MS), tandem mass spectrometry (MS-MS), and/or MS3 analysis, or the step of (e) utilizing a search program to substantially match the spectra obtained for the modified peptide during the characterization of step (d) with the spectra for a known peptide sequence, thereby identifying the parent protein(s) of the modified peptide.
    Type: Application
    Filed: June 28, 2007
    Publication date: September 27, 2012
    Inventors: John Rush, Hui Zhang, Xiangming Zha, Michael J. Comb, Yi Tan
  • Publication number: 20120178149
    Abstract: Methods of preparing plant-derived virus like particles (VLPs) are provided. The method may comprise obtaining a plant, or plant matter comprising apoplast-localized VLPs, producing a protoplast/spheroplast fraction and apoplast fraction from the plant or plant matter, and recovering the apoplast fraction. The apoplast fraction comprises plant-derived VLPs. Alternatively, VLPs may be obtained from plant or plant matter comprising plant-derived VLPs by digesting the plant matter using a cell wall degrading enzyme composition to produced a digested fraction. The digested fraction is filtered to produced a filtered fraction, and the plant-derived VLPs are recovered from the filtered fraction.
    Type: Application
    Filed: September 21, 2010
    Publication date: July 12, 2012
    Applicant: MEDICAGO INC.
    Inventors: Louis-Philippe Vezina, Manon Couture, Dany Paquet, Michele Dargis, Marc-Andre D'Aoust
  • Publication number: 20120164648
    Abstract: The invention of Integrated Versatile and Systems Preparation of Specimens relates an open module technology which integrates synchronously the methods of protection, isolation and alteration of specimens into the “One for All” product or kit. The product or kit comprises a core module without or with Plug-in modules and a set of comprehensive protocols which can simultaneously or individually isolate systems biomolecules including DNA/ccfDNA, Large RNA/mRNA/ccfRNA, Small RNA/miRNA/ccfmiRNA, Protein, Lipid, Carbohydrates, and Metabolite versatilely from a vast variety of specimens including solid specimens and liquid specimens. The product or kit can accept new and custom Plug-in modules to expand functions and applications.
    Type: Application
    Filed: December 28, 2010
    Publication date: June 28, 2012
    Applicant: BEXMART
    Inventors: Bob Han, Eric Han, Xiaohui Xiong, Xiaoliang Han
  • Publication number: 20120083027
    Abstract: The invention is related to a method for purification of recombinant proteins using highly basic proteins from thermophilic bacteria as purification tags for use in a cation-exchange chromatography purification step. The basic proteins may be ribosomal proteins. The recombinant proteins are expressed in eukaryotic or prokaryotic host cells. The purification tag will typically have a pl above about 9 and comprise from about 15 to about 250 amino acid residues.
    Type: Application
    Filed: December 1, 2011
    Publication date: April 5, 2012
    Applicant: Novo Nordisk A/S
    Inventors: Allan Christian Shaw, Susanne Bang, Jing Su
  • Patent number: 8124396
    Abstract: The formation of acrylamide during heat treatment in the production of a food product is reduced by treating the raw material with an enzyme before the heat treatment. The enzyme is capable of reacting on asparagine or glutamine (optionally substituted) as a substrate or is a laccase or a peroxidase.
    Type: Grant
    Filed: December 7, 2007
    Date of Patent: February 28, 2012
    Assignee: Novozymes A/S
    Inventors: Gitte Budolfsen, Morten Tovborg Jensen, Hans Peter Heldt-Hansen, Mary Ann Stringer, Lene Lange
  • Publication number: 20120040080
    Abstract: Methods for producing a biofuel precursor are provided. Also provided are biocatalysts that convert a feedstock to a biofuel precursor.
    Type: Application
    Filed: October 7, 2011
    Publication date: February 16, 2012
    Applicant: GEVO, INC.
    Inventors: Andrew C. Hawkins, David A. Glassner, Thomas Buelter, James Wade, Peter Meinhold, Matthew W. Peters, Patrick R. Gruber, William A. Evanko, Aristos A. Aristidou
  • Publication number: 20120039813
    Abstract: This present invention proposes a method of producing intrinsically colored, luminescent silk fibroin by feeding silkworms with feed comprising the luminescent dyes as well as the appropriate degumming process to remove sericin while retaining the dyes in silk fibroin are also described. Luminescent and functional molecular organic dyes with balanced hydrophobic/hydrophobic properties that can be effectively absorbed into silk glands of the silkworm are also described.
    Type: Application
    Filed: February 17, 2010
    Publication date: February 16, 2012
    Inventors: Natalia Chendrawati Tansil, Ming-Yong Han, Xiang Yang Liu, Radiana Soh, Yang Li