Carbohydrate Material Recovered Or Purified Patents (Class 435/274)
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Publication number: 20110195426Abstract: The present invention is partly based on the discovery that adverse factors can prevent an effective extraction of nucleic acids from a biological sample and that novel and unexpected agents and steps may be used to mitigate or remove the adverse factors, thereby dramatically improving the quality of the extracted nucleic acids. As such, one aspect of this invention is a novel method for extracting high quality nucleic acids from a biological sample. The high quality extractions obtained by the novel methods described herein are characterized by high yield and high integrity, making the extracted nucleic acids useful for various applications in which high quality nucleic acid extractions are preferred, e.g., a diagnosis, prognosis or therapy evaluation for a medical condition.Type: ApplicationFiled: July 16, 2010Publication date: August 11, 2011Applicant: THE GENERAL HOSPITAL CORPORATIONInventors: Leileata M. Russo, Kevin C. Miranda, Johan Skog
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Patent number: 7976882Abstract: The present invention relates to demucilaged flax sprouts derived from flaxseeds freed of their mucilage. The flax sprouts according to the invention are easily digestible and can be utilized in different fields, e.g. in food industry, therapy and hus-handry. The invention also relates to the production process and applications of the demucilaged flax sprouts. The present invention further relates to a process for recovering mucilaginous substance generated as a by-product in the production process as well as to various applications thereof.Type: GrantFiled: September 6, 2005Date of Patent: July 12, 2011Inventor: Ferenc Föglein
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Publication number: 20110104814Abstract: The invention provides recombinant B. thetaiotaomicron GAG lyase polypeptides. The invention also provides nucleic acid molecules encoding such polypeptides, recombinant expression vectors containing B. thetaiotaomicron GAG lyase nucleic acid molecules, and host cells into which the expression vectors have been introduced. Characterization, diagnostic and therapeutic methods utilizing compositions of the invention are also provided.Type: ApplicationFiled: December 21, 2010Publication date: May 5, 2011Inventor: James Myette
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Patent number: 7858353Abstract: Isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius are provided. Further provided are methods of at least partially degrading, cleaving, or removing polysaccharides, lignocellulose, cellulose, hemicellulose, lignin, starch, chitin, polyhydroxybutyrate, heteroxylans, glycosides, xylan-, glucan-, galactan, or mannan-decorating groups using isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius.Type: GrantFiled: January 29, 2009Date of Patent: December 28, 2010Assignee: Battelle Energy Alliance, LLCInventors: David N. Thompson, William A. Apel, Vicki S. Thompson, David W. Reed, Jeffrey A. Lacey, Emily D. Henriksen
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Publication number: 20100190872Abstract: Disclosed are methods for producing yeast ?-glucan and mannan preparations. The methods employ an autolysis process, followed by enzymatic treatment with one or more of a protease, glucanase or lipase. The preparations produced may be used in food supplements, pharmaceuticals, cosmetics, animal feeds, and neutraceuticals.Type: ApplicationFiled: January 25, 2010Publication date: July 29, 2010Applicant: SENSIENT FLAVORS INC.Inventor: Joseph James Sedmak
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Publication number: 20100047895Abstract: The present invention relates to a method for the selective purification of bacterial cells and/or cell components, whereby the purification is performed by means of a solid support.Type: ApplicationFiled: July 28, 2009Publication date: February 25, 2010Applicants: Hyglos Invest GmbH, bioMerieux S.A.Inventors: Michael Schütz, Renate Grassl, Roman Meyer, Sibylle Frick, Ingrid Robl, Thomas Zander, Stefan Miller
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Publication number: 20100031400Abstract: The present invention relates to isolated polypeptides having acetylxylan esterase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: July 31, 2009Publication date: February 4, 2010Applicant: Novozymes A/SInventors: Michelle Maranta, Kimberly Brown
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Publication number: 20090324574Abstract: The invention provides hydrolases, polynucleotides encoding them, and methods of making and using these polynucleotides and polypeptides. In one aspect, the invention is directed to polypeptides, e.g., enzymes, having a hydrolase activity, e.g., an esterase, acylase, lipase, phospholipase (e.g., phosphplipase A, B, C and D activity, patatin activity, lipid acyl hydrolase (LAH) activity) or protease activity, including thermostable and thermotolerant hydrolase activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The hydrolase activities of the polypeptides and peptides of the invention include esterase activity, lipase activity (hydrolysis of lipids), acidolysis reactions (to replace an esterified fatty acid with a free fatty acid), transesterification reactions (exchange of fatty acids between triglycerides), ester synthesis, ester interchange reactions, phospholipase activity and protease activity (hydrolysis of peptide bonds).Type: ApplicationFiled: February 2, 2007Publication date: December 31, 2009Applicant: Verenium CorporationInventors: Eric J. Mathur, Walter N. Callen, Roderick Fielding
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Publication number: 20090318382Abstract: A method comprises preventing or inhibiting bacterial adhesion and/or bacterial biofilm development by treating a substrate with a composition of a soluble group II capsular polysaccharide obtained from a bacterial strain.Type: ApplicationFiled: June 25, 2007Publication date: December 24, 2009Applicants: INSTITUT PASTEUR, CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUEInventors: Jean-Marc Ghigo, Jaione Valle, Sandra Da Re
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Publication number: 20090269745Abstract: The present invention provides a method for inactivating RNase which generally presents in a sample such as biological sample (especially an excrement sample), or in a sample such as a living body-derived sample (especially an excrement-derived sample) obtained by separation of an RNA-including body therefrom or the like; a method for extracting and detecting RNA from the sample. An RNA extraction method, comprising the steps of: obtaining a mixture under a heating condition, said mixture comprising: a sample comprising an RNA-including body and RNase, and an alkaline treating reagent comprising at least a reducing agent, and having pH of 8.1 or higher, and conducting inactivation of the RNase and extraction of RNA from the RNA-including body by keeping the mixture under the heating condition. An RNA detection method, comprising conducting RNA amplification reaction by mixing a treated sample liquid comprising RNA extracted by the extraction method and an amplification reaction solution.Type: ApplicationFiled: November 2, 2006Publication date: October 29, 2009Inventors: Hiroshi Tonoike, Yoshinari Shirasaki, Naoyuki Nishimura, Shigeru Tamatsukuri, Kuhomi Watanabe, Yasuhiko Sakakura, Hiroyuki Nakayama
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Patent number: 7604974Abstract: The present invention provides a novel cellulase nucleic acid sequence, designated 029cel, and the corresponding 029cel amino acid sequence. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding 029cel, recombinant 029cel proteins and methods for producing the same.Type: GrantFiled: April 28, 2004Date of Patent: October 20, 2009Assignee: Genencor International, Inc.Inventors: Brian E. Jones, William D. Grant, Shaun Heaphy, Susan Grant
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Publication number: 20090203107Abstract: Isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius are provided. Further provided are methods of at least partially degrading, cleaving, or removing polysaccharides, lignocellulose, cellulose, hemicellulose, lignin, starch, chitin, polyhydroxybutyrate, heteroxylans, glycosides, xylan-, glucan-, galactan, or mannan-decorating groups using isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius.Type: ApplicationFiled: January 29, 2009Publication date: August 13, 2009Applicant: Battelle Energy Alliance, LLCInventors: David N. Thompson, William A. Apel, Vicki S. Thompson, David W. Reed, Jeffrey A. Lacey, Emily D. Henriksen
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Patent number: 7560126Abstract: In one aspect, the invention is directed to polypeptides having an amylase activity, polynucleotides encoding the polypeptides, and methods for making and using these polynucleotides and polypeptides. In one aspect, the polypeptides of the invention can be used as amylases, for example, alpha amylases, to catalyze the hydrolysis of starch into sugars.Type: GrantFiled: March 6, 2003Date of Patent: July 14, 2009Assignee: Verenium CorporationInventors: Walter Callen, Toby Richardson, Gerhard Frey, Kevin A. Gray, Janne S. Kerovuo, Malgorzata Slupska, Nelson R. Barton, Eileen O'Donoghue, Eric J. Mathur, Jay M. Short
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Publication number: 20090176296Abstract: The present invention relates to a process for isolating total nucleic acid from a nucleic acid-containing sample and a kit for carrying out said process. More specifically, it relates to the isolation of RNA from a nucleic-acid containing sample.Type: ApplicationFiled: June 9, 2007Publication date: July 9, 2009Applicant: QIAGEN GmbHInventors: Vidar Skagestad, Dyre Kamfjord
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Publication number: 20090155238Abstract: The invention relates to enzymes having xylanase, mannanase and/or glucanase activity, e.g., catalyzing hydrolysis of internal ?-1,4-xylosidic linkages or endo-?-1,4-ghicanase linkages; and/or degrading a linear polysaccharide beta-1,4-xylan into xylose. Thus, the invention provides methods and processes for breaking down hemicellulose, which is a major component of the cell wall of plants, including methods and processes for hydrolyzing hemicelluloses in any plant or wood or wood product, wood waste, paper pulp, paper product or paper waste or byproduct. In addition, methods of designing new xylanases, mannanases and/or glucanases and methods of use thereof are also provided. The xylanases, mannanases and/or glucanases have increased activity and stability at increased pH and temperature.Type: ApplicationFiled: February 14, 2007Publication date: June 18, 2009Applicant: Verenium CorporationInventors: David Weiner, David Blum, Alexander Varvak, Shaun Healey, Kristine Chang, Geoff Hazlewood, Thomas Todaro, Grace Desantis, Hwai Chang, Connie Jo Hansen, Scott W. Beaver, Thomas Woodward, Charles Hancock
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Publication number: 20090155397Abstract: The present invention relates to demucilaged flax sprouts derived from flaxseeds freed of their mucilage. The flax sprouts according to the invention are easily digestible and can be utilized in different fields, e.g. in food industry, therapy and hus-handry. The invention also relates to the production process and applications of the demucilaged flax sprouts. The present invention further relates to a process for recovering mucilaginous substance generated as a by-product in the production process as well as to various applications thereof.Type: ApplicationFiled: September 6, 2005Publication date: June 18, 2009Applicant: Biogreen A/SInventor: Ferenc Föglein
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Publication number: 20090130256Abstract: There is disclosed a method of decomposing fermentation-inhibiting substances in a fluid including saccharide-containing substances, wherein a microbiotic mixture, especially a mixture of photosynthetically working micro-organisms and light-emitting micro-organisms is introduced into the fluid, the mixture being intended to decompose the fermentation-inhibiting substances.Type: ApplicationFiled: March 9, 2007Publication date: May 21, 2009Applicant: Georg Fritzmeier GmbH & Co. KGInventor: Christian Uphoff
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Patent number: 7510857Abstract: A Clostridium thermocellum thermostable cellulase enzyme with both endocellulase activity and exocellulase activity that is able to degrade cellulose in the absence of scaffolding and other cellulosomic proteins is provided. The use of the enzyme to degrade cellulosic materials to soluble sugars is also provided.Type: GrantFiled: April 27, 2007Date of Patent: March 31, 2009Assignee: C5-6 Technologies, Inc.Inventor: Phillip Brumm
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Publication number: 20090078384Abstract: The invention provides polypeptides coding for new esterases from rumen. The invention also relates to functional fragments or functional derivatives thereof as well as to nucleic acids encoding the polypeptides of the invention, vectors and host cells containing said nucleic acids, a method for producing the polypeptides and the use of the polypeptides according to the present invention for various industrial purposes and medical treatments. The invention also relate to the conversion of said esterases into lipases.Type: ApplicationFiled: July 6, 2005Publication date: March 26, 2009Inventors: Manuel Ferrer, Peter Golyshin, Olga Golyshina, Tatyana Chernikova, Carsten Strompl, Kenneth Timmis, Kieran Elborough, Graeme Jarvis
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Patent number: 7504120Abstract: The invention relates to xylanases and to polynucleotides encoding the xylanases. In addition, methods of designing new xylanases and methods of use thereof are also provided. The xylanases have increased activity and stability at increased pH and temperature.Type: GrantFiled: June 16, 2003Date of Patent: March 17, 2009Assignee: Verenium CorporationInventors: Brian Steer, Walter Callen, Shaun Healey, Geoff Hazlewood, Di Wu, David Blum, Alireza Esteghlalian
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Publication number: 20080299646Abstract: The invention provides a process for producing polysaccharide gum comprising extracting a plant material with an aqueous solvent to produce a polysaccharide gum-containing extract; contacting the extract with at least one proteolytic enzyme to at least partially digest proteins in the extract; adding an organic solvent to the extract to precipitate the polysaccharide gum; and collecting the precipitated polysaccharide gum.Type: ApplicationFiled: July 12, 2006Publication date: December 4, 2008Inventors: Peter R. Chang, Debbie P. Anderson, Tara C. McIntosh, Neil D. Westcott
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Publication number: 20080268527Abstract: The invention relates to mutant strains of the genus Sphingomonas which have a mutation in at least one gene encoding a protein involved in polyhydroxybutyrate (“PHB”) synthesis that allows the mutant strains to produce PHB-deficient sphingans. The invention is also directed to a process for preparing a clarified sphingan solution comprising heating aqueous sphingan solution, in particular PHB-deficient sphingan solution, to a clarification temperature of about 30° C. to about 70° C., and treating the solution with a clarification agent and enzymes. In addition, the invention is directed to a food or industrial product comprising a PHB-deficient and/or clarified sphingan. One particular embodiment of the invention is directed to a clarified, PHB-deficient high-acyl gellan and the processes of making thereof.Type: ApplicationFiled: December 2, 2005Publication date: October 30, 2008Applicant: C.P. Kelco U.S., Inc.Inventors: Stan Bower, Ellen Burke, Nancy E. Harding, Yamini N. Patel, J. Carrie Schneider, Dagmar Meissner, Neil A. Morrison, Ralph Bezanson
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Publication number: 20080182323Abstract: Disclosed are processes for liquefaction and saccharification of polysacharide containing biomasses having high dry matter content (>20%) and preferably possessing large average particle size. The polysaccharide containing biomasses are subject to enzymatic hydrolysis in a mixer that utilizes “free fall” mixing. “Free fall” mixing provides mechanical degradation of the biomass during hydrolysis and, in the case of lignocellulosic biomass, promotes movement of cellulase enzymes along cellulose chains, which improves enzymatic hydrolysis at high dry matter.Type: ApplicationFiled: April 15, 2008Publication date: July 31, 2008Applicants: ELSAM ENGINEERING A/S, KOBENHAVNS UNIVERSITETInventors: Claus Felby, Jan Larsen, Henning Jergensen, Jakob Vibe-Pedersen
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Patent number: 7396670Abstract: The formation of acrylamide during heat treatment in the production of a food product is reduced by treating the raw material with an enzyme before the heat treatment. The enzyme is capable of reacting on asparagine or glutamine (optionally substituted) as a substrate or is a laccase or a peroxidase.Type: GrantFiled: October 10, 2003Date of Patent: July 8, 2008Assignee: Novozymes A/SInventors: Gitte Budolfsen, Morten Tovborg Jensen, Hans Peter Heldt-Hansen, Mary Ann Stringer
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Patent number: 7323326Abstract: Genes encoding novel cellulases, and a gene encoding a protein that facilitates the action of such novel cellulases, the novel cellulases and a protein that facilitates the action of such cellulases, and enzyme preparations containing such proteins are described. The native hosts and the culture medium of said hosts containing said novel cellulases are also disclosed. These proteins are especially useful in the textile and detergent industry and in pulp and paper industry.Type: GrantFiled: February 20, 2004Date of Patent: January 29, 2008Assignee: AB Enzymes OyInventors: Arja Miettinen-Oinonen, John Londesborough, Jari Vehmaanpera, Heli Haakana, Arja Mantyla, Raija Lantto, Minna Elovainio, Vesa Joutsjoki, Marja Paloheimo, Pirkko Suominen
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Patent number: 7309587Abstract: The present invention can provide a process for producing a protein having ?1,3-N-acetylglucosaminyltransferase activity using a transformant comprising a DNA encoding a protein having ?1,3-N-acetylglucosaminyltransferase activity derived from a microorganism belonging to the genus Pasteurella and a process for producing an N-acetylglucosamine-containing complex carbohydrate using a transformant capable of producing a protein having ?1,3-N-acetylglucosaminyltransferase activity derived from a microorganism.Type: GrantFiled: October 25, 2002Date of Patent: December 18, 2007Assignee: Kyowa Jakko Kogyo Co., Ltd.Inventors: Tetsuo Endo, Satoshi Koizumi
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Patent number: 7294498Abstract: A thermostable glycosidase enzymes derived from various Thermococcus, Staphylothermus and Pyrococcus organisms is disclosed. The enzymes are produced from native or recombinant host cells and can be utilized in the food processing industry, pharmaceutical industry and in the textile industry, detergent industry and in the baking industry.Type: GrantFiled: April 9, 2002Date of Patent: November 13, 2007Assignee: Verenium CorporationInventors: Edward J. Bylina, Ronald V. Swanson, Eric J. Mathur, David E. Lam
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Patent number: 7273748Abstract: Genes encoding novel cellulases, and a gene encoding a protein that facilitates the action of such novel cellulases, the novel cellulases and a protein that facilitates the action of such cellulases, and enzyme preparations containing such proteins are described. The native hosts and the culture medium of said hosts containing said novel cellulases are also disclosed. These proteins are especially useful in the textile and detergent industry and in pulp and paper industry.Type: GrantFiled: April 16, 2004Date of Patent: September 25, 2007Assignee: AB Enzymes OyInventors: Arja Miettinen-Oinonen, John Londesborough, Jari Vehmaanpera, Heli Haakana, Arja Mantyla, Raija Lantto, Minna Elovainio, Vesa Joutsjoki, Marja Paloheimo, Pirkko Suominen
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Patent number: 7256030Abstract: An enzyme exhibiting endo-?-1,4-glucanase activity which belongs to family 9 of glycosyl hydrolases is obtainable from or endogeneous to a strain belonging to the genus Bacillus such as Bacillus licheniformis, ATCC 14580; an isolated polynucleotide (DNA) molecule encoding an enzyme or enzyme core (the catalytically active domain of the enzyme) exhibiting endo-?-1,4-glucanase activity selected from (a) polynucleotide molecules comprising a nucleotide sequence as shown in SEQ ID NO:1 from nucleotide 76 to nucleotide 1455 or from nucleotide 76 to nucleotide 1941, (b) polynucleotide molecules that encode a polypeptide being at least 75% identical to the amino acid sequence of SEQ ID NO:2 from amino acid residue 26 to amino acid 485 or from amino acid residue 26 to amino acid residue 646, and (c) degenerate nucleotide sequences of (a) or (b), the expressed endoglucanase enzyme being useful in various industrial applications.Type: GrantFiled: May 23, 2000Date of Patent: August 14, 2007Assignee: Novozymes A/SInventors: Martin Schülein, Mads Eskelund Bjørnvad
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Patent number: 7144722Abstract: The present invention relates to pectate lyases comprising the amino acid sequence Asn Leu Asn Ser Arg Val Pro (NLNSRVP) (amino acids 236–242 of SEQ ID NO: 2) belonging to Family 1 of polysaccharide lyases have good performance in industrial processes under neutral or alkaline conditions such as laundering and textile processing. The pectate lyase may be derivable from Bacillus species.Type: GrantFiled: September 4, 2003Date of Patent: December 5, 2006Assignee: Novozymes A/SInventors: Lene Nonboe Andersen, Hanne Dela, legal representative, Niels Erik Krebs Lange, Mads Eskelund Bjornvad, Soren Moller, Sanne O Schroder Glad, Markus Sakari Kauppinen, Kirk Schnorr, Lars Kongsbak, Martin Schulein, deceased
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Patent number: 7063976Abstract: A process for preparing beta-fructofuranosidase enzyme and a process for producing fructooligosaccharides, in which the preparation of the enzyme is obtained by cultivating the fungus Aspergillus niger, either wild or mutated, in a preferably semi-solid culture medium, in order to produce an extracellular enzyme, which is submitted to transfructosylation for producing fructooligosaccharides comprising sugars which are formed by one unit of sacrose and by two, three and four units of fructose.Type: GrantFiled: July 15, 2002Date of Patent: June 20, 2006Assignee: Usina da Barra S/A—Acucar e AlcoolInventors: Yong Kun Park, Gláucia Maria Pastores
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Patent number: 6998262Abstract: This invention provides a Dioscorea polysaccharide extract prepared by the method of this invention, which enhances the immunological activities by regulating the gene expression of cytokines. This invention further provides a Dioscorea polysaccharide extract, which has a synergistic effect with an oral vaccine and is capable of eliminating oral tolerance.Type: GrantFiled: May 30, 2002Date of Patent: February 14, 2006Assignee: National Yang-Ming UniversityInventor: Rong-Tsun Wu
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Patent number: 6897046Abstract: The yield of active polysaccharides contained in Agaricus blazei, such as ?-glucan, can be improved, by decomposing and treating the mycelium or fruit body of Agaricus blazei or a liquid waste of the culture of the mycelium with an enzymatic agent principally comprising hemicellulase. By increasing the concentrations of the active polysaccharides, furthermore, the digestion and absorption of the biologically active substance of the present invention after the incorporation thereof into bodies is prominently elevated, so that the active substance can exert excellent immune activation effect on cancers and other adult diseases.Type: GrantFiled: September 3, 2002Date of Patent: May 24, 2005Assignee: Japan Applied Microbiology Research Institute Ltd.Inventors: Isao Horiuchi, Takeru Suyama, Tsunehisa Morio
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Patent number: 6835558Abstract: An efficient method for producing high quality beta glucan enriched soluble dietary fiber products is described. An aqueous extraction slurry of beta glucan-containing grain material is homogenized, acidified and enzymatically digested to reduce viscosity and optimize separation of insolubles from the aqueous extract solution. The resulting extract is heat processed to precipitate denatured protein components and subsequently dried or otherwise processed to provide the products for use as food additives or dietary supplements.Type: GrantFiled: February 4, 2002Date of Patent: December 28, 2004Assignee: General Mills, Inc.Inventors: Bernhard H. Van Lengerich, Olaf Gruess, Friedrich P. Meuser
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Patent number: 6706497Abstract: The present invention provides methods for producing sialyloligosaccharides in situ in dairy sources and cheese processing waste streams, prior to, during, or after processing of the dairy source during the cheese manufacturing process. The methods of the present invention use the catalytic activity of &agr;(2-3) trans-sialidases to exploit the high concentrations of lactose and &agr;(2-3) sialosides which naturally occur in dairy sources and cheese processing waste streams to drive the enzymatic synthesis of &agr;(2-3) sialyllactose. &agr;(2-3) sialyloligosaccharides produced according to these methods are additionally encompassed by the present invention. The invention also provides for recovery of the sialyloligosaccharides produced by these methods. The invention further provides a method for producing &agr;(2-3) sialyllactose. The invention additionally provides a method of enriching for &agr;(2-3) sialyllactose in milk using transgenic mammals that express an &agr;(2-3) trans-sialidase transgene.Type: GrantFiled: September 18, 2001Date of Patent: March 16, 2004Assignee: Neose Technologies, Inc.Inventors: Marc Pelletier, William A. Barker, David J. Hakes, David A. Zopf
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Patent number: 6623953Abstract: The invention relates to an enzymatic composition and also to a method for seasoning wood, intended in particular for applications in the food industry. The method consists in immersing green wood in an aqueous solution comprising an enzymatic composition. The wood can subsequently be dried very rapidly in a drying facility. The invention makes it possible to improve the aromatic characteristics of the wood by eliminating certain substances judged to be undesirable due to their astringent nature or bitter nature, and transforming them into neutrally-tasting molecules.Type: GrantFiled: June 29, 2001Date of Patent: September 23, 2003Assignee: ETS. Robert Stiernon S.A.Inventor: Henry Olivier
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Patent number: 6623954Abstract: The invention relates to a method of removing phosphorous from sialyloligosaccharides isolated from a dairy stream using phytase.Type: GrantFiled: May 28, 2002Date of Patent: September 23, 2003Assignee: Neose Technologies, Inc.Inventors: Michael Eugene Spade, Jonathan Kim Weil, Michael Scott McHale
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Patent number: 6623750Abstract: Enzyme feed supplements containing fungal cellulase produced by Trichoderma viride are specially adapted for increasing protein digestability of dairy cow and cattle feed. Preferred embodiments of the present invention include enzyme formulations adapted for application to corn and enzyme formulations adapted for application to grain sorghum, to which is added Trichoderma viride cellulase. An increase in protein digestability has been evidenced by increased protein content of milk and increased nitrogen retention from the feed.Type: GrantFiled: July 27, 2000Date of Patent: September 23, 2003Assignees: Loveland Industries, Inc., George A. Jeffreys & Co., Inc.Inventors: Charles W. Cobb, James F. Tobey
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Patent number: 6613754Abstract: In a process for preparing a polysaccharide-based extract, raw material of ganoderma genus is subjected to an alcohol treatment to obtain a polysaccharide-rich product from which a supernatant solution is subsequently obtained with the use of water of an elevated temperature as an extracting agent. Thereafter, alcohol is added to the supernatant solution to precipitate the polysaccharide-based extract. The polysaccharide-based extract can be used in an orally active medicinal product which has immunopotentiating and antitumoral effects.Type: GrantFiled: September 22, 2000Date of Patent: September 2, 2003Assignee: National Yang-Ming UniversityInventor: Rong-Tsun Wu
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Patent number: 6607725Abstract: A conjugate vaccine for Nontypeable Haemophilus influenzae comprising lipooligosaccharide from which esterified fatty acids have been removed conjugated to an immunogenic carrier. The vaccine is useful for prevention of otitis media and respiratory infections in mammals.Type: GrantFiled: February 20, 2001Date of Patent: August 19, 2003Assignee: The United States of America as represented by the Department of Health and Human ServicesInventors: Xin-Xing Gu, Chao-Ming Tsai, David J. Lim, John B. Robbins
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Patent number: 6586213Abstract: A process is disclosed for clarifying an aqueous xanthan gum solution comprising treatment of the xanthan solution with at least one chelating agent, surfactant, organic acid, or a mixture thereof, and with a protease enzyme or a lysozyme and a protease enzyme. Also disclosed is a highly purified solid xanthan gum and compositions containing the same, wherein the gum is obtained from the clarified xanthan gum solution.Type: GrantFiled: April 13, 2001Date of Patent: July 1, 2003Assignee: CP Kelco U.S., Inc.Inventors: Joseph M. Kobzeff, Daniel E. Mead, Todd A. Talashek, Don DiMasi
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Patent number: 6586212Abstract: The invention provides a method of obtaining a cellulose material from corn fiber wherein the method comprises the steps of: (a) heating a mixture of corn fiber and a liquid; (b) contacting the mixture of step (a) with a protease enzyme, thereby providing a proteolyzed corn fiber and a liquid; (c) separating the liquid from the proteolyzed corn fiber; (d) contacting the proteolyzed corn fiber at least once with an alkaline extractant, thereby providing an insoluble cellulose material and a first liquid comprising arabinoxylan; (e) separating the insoluble cellulose material from the first liquid comprising arabinoxylan at a temperature of at or above about 60° C.; and (f) rinsing the insoluble cellulose material to remove essentially all alkali, thereby providing a cellulose material having a cellulose content of at least about 50% and consisting essentially of cellulose I. Cellulose esters and ethers are also prepared from the derivatizable cellulose prepared according to the methods herein.Type: GrantFiled: February 10, 2000Date of Patent: July 1, 2003Assignee: Eastman Chemical CompanyInventors: Charles M. Buchanan, Norma L. Buchanan, John S. Debenham, Michael C. Shelton, Matthew D. Wood
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Patent number: 6558930Abstract: The present invention relates to physiologically active materials separated from the cereals and manufacturing method thereof. Physiologically active materials such as ferulic acid and arabinoxylan present in cereal brans were separated by the extrusion process and the subsequent treatment with plant cell wall hydrolyzing enzymes. This combined process of extrusion and enzyme treatments for cereal brans, compared to the individual treatment, significantly increased the separation efficiency of physiologically active materials in cereal brans, ferulic acid and arabinoxylan, which inherently exist as insoluble materials in the cell wall of cereal bran.Type: GrantFiled: June 8, 2001Date of Patent: May 6, 2003Inventors: Jaekwan Hwang, Bosun Park, Jungmi Yun
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Patent number: 6506592Abstract: Recombinant, thermostable alpha-glucosidases from archaeal micro-organisms and isolated DNA encoding for such alpha-glucosidases are provided. The isolated DNA is obtained by use of DNA or antibody probes prepared from the DNA encoding S. sulfataricus alpha-glucosidase. Also provided are methods for producing recombinant archaeal thermostable alpha-glucosidase and transformants incorporating thermostable alpha-glucosidase. Autoprocessing of plant tissue through the use of transgenic thermostable glycosyl hydrolases is described.Type: GrantFiled: August 18, 1999Date of Patent: January 14, 2003Assignee: Board of Regents of the University of NebraskaInventor: Paul Blum
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Patent number: 6485954Abstract: The present invention relates to an enzyme with galactanase activity, a DNA construct encoding the enzyme with galactanase activity, a method of producing the enzyme, an enzyme composition comprising said enzyme with galactanase activity, and the use of said enzyme and enzyme composition for a number of industrial applications.Type: GrantFiled: November 28, 2000Date of Patent: November 26, 2002Assignee: Novozymes A/SInventors: Lene Venke Kofod, Markus Sakari Kauppinen, Lene Nonboe Andersen, Ib Groth Clausen, Anette Müllertz
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Patent number: 6465218Abstract: The yield of active polysaccharides contained in Agaricus blazei, such as &bgr;-glucan, can be improved, by decomposing and treating the mycelium or fruit body of Agaricus blazei or a liquid waste of the culture of the mycelium with an enzymatic agent principally comprising hemicellulase. By increasing the concentrations of the active of the biologically active substance of the present invention after the incorporation thereof into bodies is prominently elevated, so that the active substance can exert excellent immune activation effect on cancers and other adult diseases.Type: GrantFiled: January 6, 2000Date of Patent: October 15, 2002Assignee: Japan Applied Microbiology Research Institute Co., Ltd.Inventors: Isao Horiuchi, Takeru Suyama, Tsunehisa Morio
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Publication number: 20020137922Abstract: This invention describes improvements in or relating to the production of polysaccharide derivatives; more particularly it relates to a method of converting insoluble polysaccharides to a form in which they form part of an anhydrous system in which they may be used as chemical intermediates in the production of derivativesType: ApplicationFiled: June 14, 1999Publication date: September 26, 2002Inventor: DANIEL RICHARD HLYNIANSKI
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Patent number: 6426211Abstract: A purified xylanase derived from B. Pumilus PRL B12 is disclosed. This xylanase is efficient for use in the biobleaching of wood pulp, permitting a strong reduction in the quantity of chlorine used and AOX compounds produced in classical and ECF wood pulp bleaching sequences as well as the quantity of ozone used in TCF sequences. The gene coding for the xylanase was isolated and purified and used to construct an expression vector therefor. A recombinant host strain of B. licheniformis is also disclosed which is efficient for expressing heterologous enzymes, including the xylanase when transformed by the expression vector.Type: GrantFiled: May 5, 1998Date of Patent: July 30, 2002Assignee: Genencor International Inc.Inventors: Eric de Buyl, Andrée Lahaye, Pierre Ledoux, Antoine Amory, René Detroz, Christophe Andre, Roman Vetter
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Patent number: 6376220Abstract: The gist of the present invention resides in a mannose-containing copra meal composition obtained by allowing two enzymes, xylanase and &bgr;-galactomannan, to act on copra meal, and in a method for preparing the same, in which mannose can be liberated efficiently and economically by combining the two enzymes. A decreasing effect against Salmonella is expected at an economical cost, by adding into feeds the mannose-containing copra meal composition according to the present invention or mannoses obtained from the composition by extraction.Type: GrantFiled: August 13, 2001Date of Patent: April 23, 2002Assignee: Fujii Oil Co., Ltd.Inventor: Futoshi Yokomizo
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Patent number: 6352845Abstract: In one aspect, the invention provides methods of obtaining at least one monosaccharide from corn fiber the method comprises the steps of: (a) heating an aqueous mixture of corn fiber and a liquid; (b) contacting the mixture of step (a) with a protease enzyme, thereby providing a proteolyzed corn fiber and a liquid; (c) separating the liquid from the proteolyzed corn fiber; (d) contacting the proteolyzed corn fiber at least once with an alkaline extractant, thereby providing an insoluble cellulose material and a liquid comprising arabinoxylan; (e) separating the insoluble cellulose material from the liquid comprising arabinoxylan; (f) reducing the volume of the liquid comprising arabinoxylan by removing excess alkaline extractant, thereby providing a concentrated liquid comprising from about 10 to about 50% solids, wherein the solids comprise arabinoxylan; and (g) hydrolyzing the arabinoxylan from step (f) in the presence of a catalyst and a solvent, thereby providing a mixture comprising at least one monosaccType: GrantFiled: February 10, 2000Date of Patent: March 5, 2002Assignee: Eastman Chemical CompanyInventors: Charles M. Buchanan, Norma L. Buchanan, John S. Debenham, Michael C. Shelton, Matthew D. Wood, Michael J. Visneski, Bhaskar K. Arumugam, John K. Sanders, Larry R. Lingerfelt, Jr., Larry Blair