Bubble Bioreactor Patents (Class 435/296.1)
  • Patent number: 9631219
    Abstract: Described and claimed is a novel system and method for monitoring the metabolic rate of a cellular population in an industrial process. The invention includes obtaining a sample of the cellular population and initiating a reaction between a metabolic component of the cellular population and a bioreporter. The reaction produces a detectable metabolic signal which is measured and analyzed to determine a metabolic rate of the cellular population. In preferred embodiments, a controller is operable to provide prompts to a user and/or automate various steps of the invention.
    Type: Grant
    Filed: February 24, 2010
    Date of Patent: April 25, 2017
    Assignee: Nalco Company
    Inventor: Michael E. Bradley
  • Patent number: 9012210
    Abstract: A mixing vessel (10) for containing a liquid, comprises a chamber having a lower chamber portion and an upper chamber portion wider than the lower portion, gas inlet means (14) for supplying gas to the lower portion and means for redirecting rising gas (24), such that, in use, rising gas in the form of bubbles, initially rises substantially vertically and is redirected in a substantially horizontal direction by the means for redirecting rising gas.
    Type: Grant
    Filed: December 15, 2006
    Date of Patent: April 21, 2015
    Assignee: Cellexus Limited
    Inventor: Kevin Andrew Auton
  • Patent number: 9005971
    Abstract: A container system includes a bag having of one or more sheets of flexible polymeric material, the bag having an interior surface at least partially bounding a chamber that is adapted to hold a fluid. A sparger is secured to the interior surface of the bag so that a compartment is formed between the interior surface of the bag and the sparger. At least a portion of the sparger includes a sparging sheet formed from a flexible sheet of a gas permeable material. A tubular port or tube is coupled with the bag so that a passage bounded by the tubular port or tube communicates with the compartment.
    Type: Grant
    Filed: December 2, 2013
    Date of Patent: April 14, 2015
    Assignee: Life Technologies Corporation
    Inventors: Michael E. Goodwin, Nephi D. Jones, Jeremy K. Larsen
  • Patent number: 8980623
    Abstract: A mixing vessel (10) for containing a liquid, comprises a chamber having a lower chamber portion and an upper chamber portion wider than the lower portion, gas inlet means (14) for supplying gas to the lower portion and means for redirecting rising gas (24), such that, in use, rising gas in the form of bubbles, initially rises substantially vertically and is redirected in a substantially horizontal direction by the means for redirecting rising gas.
    Type: Grant
    Filed: January 22, 2009
    Date of Patent: March 17, 2015
    Assignee: Cellexus Limited
    Inventor: Kevin Andrew Auton
  • Publication number: 20140370588
    Abstract: In one aspect, an apparatus for bioprocessing using a liquid comprises an at least partially flexible vessel for receiving the liquid, and a filter adapted for moving within the liquid. An agitator may also be provided for agitating the liquid, and may be connected to the mixer. The mixer may spin or may not spin about an axis of rotation.
    Type: Application
    Filed: December 7, 2012
    Publication date: December 18, 2014
    Inventors: Jose Antonio Castillo Gonzalez, Vishwas Pethe
  • Patent number: 8895289
    Abstract: Photochemical process and device adapted to breed, produce, or hydrocultivate microorganisms. The process includes conveying a reaction medium in a reactor in a meander-shaped way that includes moving the reaction medium along a direction that perpendicularly or inclined at an angle to an imaginary horizontal plane, wherein, during the conveying, the reaction medium moves in the reactor at least once along a first direction defined as one of a top down direction and a direction of gravity, moves in the reactor at least once along a second direction defined as one of a bottom up direction and against the direction of gravity, and moves in the reactor one of freely under atmospheric pressure and while exposed to the atmosphere. The process also includes introducing into and removing from the reactor the reaction medium in a continuous manner.
    Type: Grant
    Filed: January 27, 2009
    Date of Patent: November 25, 2014
    Assignee: Ecoduna AG
    Inventors: Martin Mohr, Franz Emminger
  • Patent number: 8877487
    Abstract: A photobioreactor apparatus in which a reaction chamber contains a slurry of water, nutrients and photosynthetic organisms. The sidewall of the reaction chamber is constructed of translucent material, thereby permitting light to be conveyed into the slurry to promote photosynthesis. The interior of the reaction chamber is in fluid communication with a gas dispenser. A lift member is disposed in the reaction chamber and gas is introduced in the slurry beneath the lift member, thereby providing buoyancy to lift the lift member, thereby providing agitation and movement of the slurry within the photobioreactor and enhancing growth of photosynthetic organisms.
    Type: Grant
    Filed: August 3, 2010
    Date of Patent: November 4, 2014
    Inventor: David D. Slagel
  • Patent number: 8822208
    Abstract: The bioreactor is for use in performing biological and/or biochemical reactions and includes a vessel, an agitator, a reaction assembly, and a harvesting outlet. The vessel of the bioreactor includes several ports including a mixing port, a reaction port, and a harvesting port. The agitator extends through the mixing port into the vessel while the harvesting outlet extends through the harvesting port and permits the withdrawal of reaction medium to another vessel. The reaction assembly extends through the reaction port into the vessel and has multiple components including a gas conduit adapted to introduce gas into a reaction medium in the vessel, a sampling device adapted to remove a portion of the reaction medium from the vessel without contamination of the remaining reaction medium, and an introduction conduit permitting the introduction of at least the reaction medium into the vessel.
    Type: Grant
    Filed: March 27, 2013
    Date of Patent: September 2, 2014
    Assignee: Becton, Dickinson and Company
    Inventor: Bunkim Chokshi
  • Publication number: 20140187826
    Abstract: Various embodiments provide a bioreactor for producing a bioproduct comprising a housing; and one or more catalytically active zones located in the housing and adapted to keep two incompatible gaseous reactants separated when in a gas phase, wherein each of said catalytically active zone comprises a catalytic component retainer and a catalytic component retained within and/or on the catalytic component retainer. Each of said catalytically active zones may additionally or alternatively comprise a liquid medium located on either side of the catalytic component retainer and the catalytic component may additionally or alternatively be a suspended catalytic component suspended in a liquid medium located on either side of the catalytic component retainer. Methods of using various embodiments of the bioreactor to produce a bioproduct, such as isobutanol, are also provided.
    Type: Application
    Filed: March 6, 2014
    Publication date: July 3, 2014
    Applicant: BOARD OF TRUSTEES OF MICHIGAN STATE UNIVERSITY
    Inventors: Robert Mark WORDEN, Yangmu Chloe LIU
  • Patent number: 8716010
    Abstract: A photobioreactor system is provided that comprises a bioreactor including at least two bioreactor tubes, each having an end and a hollow interior, the ends being connectively joined by one or more connector units having a hollow portion defined by a circumference, a solar concentrator configured to collect and concentrate solar power, at least one light guide associated with the solar concentrator to illuminate the hollow portion of the one or more connector units, and at least one LED illuminating the one or more connector units.
    Type: Grant
    Filed: December 15, 2010
    Date of Patent: May 6, 2014
    Assignee: GE Lighting Solutions, LLC
    Inventors: István Csányi, Laszlo Balazs, Janos Sneider, Erazmus Gerencser
  • Patent number: 8685716
    Abstract: A composting apparatus and method is described. The apparatus comprises a cylindrical drum which is horizontally rotatably supported. A hopper is disposed at a feed end of the drum for receiving organic waste material in soft or hard form. A material shredding mechanism and a grinding mechanism are rotatably secured in the hopper and driven in counter-rotation for shredding and grinding the organic waste material and feeding it into an inlet opening of the drum. A series of material mixing and conveying vane assemblies are secured spaced-apart inside the drum and define sub-composting compartments therebetween. The material mixing and conveying vane assemblies have a plurality of vanes secured in spaced-apart relationship and project interiorly towards a central longitudinal axis of the drum and are oriented at a common angle to mix, further shred and displace shredded and ground material from the inlet opening towards the rear discharge end of the drum.
    Type: Grant
    Filed: May 10, 2011
    Date of Patent: April 1, 2014
    Assignee: Great Wall of China Waste Company Inc.
    Inventor: Elias Bassile
  • Patent number: 8603805
    Abstract: A sparger includes a body bounding a compartment. A tubular port or tube is coupled with the body. The tubular port or tub bounds a passage that communicates with the compartment of the body. At least a portion of the body includes of a first sparging sheet. The first sparging sheet includes a flexible sheet of a gas permeable material such that a gas passing into the compartment of the body through the passage can exit the compartment by permeating through the first sparging sheet.
    Type: Grant
    Filed: March 20, 2006
    Date of Patent: December 10, 2013
    Assignee: HyClone Laboratories, Inc.
    Inventors: Michael E. Goodwin, Nephi D. Jones, Jeremy K. Larsen
  • Patent number: 8597939
    Abstract: The invention generally provides a cell culture vessel having at least one first zone and at least one second zone, wherein the first zone is a transfer zone for a culture medium which essentially contains no cells and the second zone is a cell culture zone. The invention further includes methods utilizing the cell culture vessel.
    Type: Grant
    Filed: March 15, 2012
    Date of Patent: December 3, 2013
    Assignee: Artelis S.A.
    Inventor: Jose Antonio Castillo Fernandez
  • Publication number: 20130309759
    Abstract: Methods, systems and apparatuses herein provide a unique and novel process to remove H2S from biogas. Methods, systems and apparatuses herein provide a unique and novel process to remove H2S from biogas with ease of operation and reduced operating and capital costs. Methods, systems and apparatuses herein provide a unique and novel process to remove H2S over CO2 from biogas.
    Type: Application
    Filed: March 15, 2013
    Publication date: November 21, 2013
    Inventors: Nicholas Kennedy, Quan-Bao Zhao, Anping Jiang, Craig Frear, Stephen W. Dvorak
  • Patent number: 8586353
    Abstract: The invention provides a device for growing genetically enhanced aquatic photoautotrophic organisms in a stable culture, causing the organisms to produce ethanol, and then separating, collecting, and removing the ethanol in situ.
    Type: Grant
    Filed: November 1, 2012
    Date of Patent: November 19, 2013
    Assignee: Algenol Biofuels Switzerland GmbH
    Inventors: Robert Paul Woods, Edwin Malkiel, Benjamin Moll, Edward Legere
  • Patent number: 8569026
    Abstract: The invention features methods for producing isoprene from cultured cells. The invention also provides compositions that include these cultured cells. The invention provides isoprene compositions, such as compositions with increased amount of isoprene or increased purity. Additionally, the invention provides methods of producing isoprene by culturing cells under conditions suitable for isoprene production while maintaining cell viability and/or metabolic activity.
    Type: Grant
    Filed: September 15, 2009
    Date of Patent: October 29, 2013
    Assignees: Danisco US Inc., The Goodyear Tire & Rubber Company
    Inventors: Gopal K. Chotani, Joseph C. McAuliffe, Caroline M. Peres, Gregory M. Whited
  • Patent number: 8501460
    Abstract: The present invention is in the field of cell bioreactors, and specifically in the field of disposable bioreactors.
    Type: Grant
    Filed: January 18, 2011
    Date of Patent: August 6, 2013
    Assignee: Mayfair Technology Limited Liability Company
    Inventor: James Francis Furey
  • Patent number: 8476067
    Abstract: A photobioreactor (100) for use in treating polluted air and producing biomass may comprise, at least in part, a generally vertical tube or fluidic pathway (102), a generally vertical helical tube or fluidic pathway (104) having a light source (106) partially positioned within the helical fluidic pathway (104), a head cap assembly (108), and a base assembly (110). In one illustrative example, the light source (106) may be a light emitting diode (LED) or a plurality of light emitting diodes (LEDs). By one approach, a gas diffusion apparatus (112) is located at the base assembly (110) adjacent the generally vertical fluidic pathway (102).
    Type: Grant
    Filed: February 1, 2008
    Date of Patent: July 2, 2013
    Assignee: Phyco2 LLC
    Inventor: Robert Morgan
  • Patent number: 8455242
    Abstract: A system for mixing a liquid solution or suspension includes a support housing bounding a compartment and a collapsible bag disposed within the compartment. A mixer is disposed within the collapsible bag while a sparger delivers a gas to the lower end of the collapsible bag. A gas outlet line extends from an upper end of the collapsible bag to a condenser assembly. A gas exhaust line and a fluid collection line both extend from the condenser assembly.
    Type: Grant
    Filed: February 22, 2010
    Date of Patent: June 4, 2013
    Assignee: HyClone Laboratories, Inc.
    Inventors: Clinton C. Staheli, Nephi D. Jones, Michael E. Goodwin
  • Patent number: 8415142
    Abstract: A method and apparatus for growing algae for sequestering carbon dioxide and then harvesting the algae includes a container for a suspension of algae in a liquid and a bioreactor having a translucent channel in fluid communication with the container to absorb CO2 and grow the algae. A monitor determines the growth of the algae in the channel. A separator separates the grown algae from the suspension and an extractor extracts biomaterials from the grown algae.
    Type: Grant
    Filed: May 28, 2008
    Date of Patent: April 9, 2013
    Inventor: Malcolm Glen Kertz
  • Patent number: 8409851
    Abstract: A bioactive filter is provided which comprises a transparent canister having gas permeable membranes as entry and exit ports. A source of carbon dioxide in gaseous form is allowed to enter the entry membrane and pass through a solution contained in the canister which supports a live colony of algae. The algae carries out photosynthesis thereby altering the carbon dioxide to oxygen and sugar. The oxygen is released through the exit port.
    Type: Grant
    Filed: December 23, 2009
    Date of Patent: April 2, 2013
    Inventor: Param Jaggi
  • Patent number: 8372632
    Abstract: A method and apparatus for sequestering CO2 using algae comprises a plurality of vertically suspended bioreactors, each bioreactor being translucent and including a flow channel formed by a plurality of baffles. A culture tank contains a suspension of water and at least one algae and includes a plurality of gas jets for introducing a CO2-containing gas into the suspension. The culture tank is in fluid communication with an inlet in each channel for flowing the suspension through the channel in the presence of light. A pump pumps the suspension into the channel inlet.
    Type: Grant
    Filed: June 13, 2007
    Date of Patent: February 12, 2013
    Inventor: Malcolm Glen Kertz
  • Publication number: 20130005011
    Abstract: A bioreactor is provided that includes a main reactor having a configuration selected from the group consisting of stirred and unstirred tank reactor, trickle bed reactor (TBR), cocurrent contactor (CCC), moving bed bioreactor (MBBR), and a bubble column reactor. The bioreactor also includes a growth reactor continuous with the main reactor and having a configuration selected from the group consisting of stirred and unstirred tank reactor, trickle bed reactor (TBR), cocurrent contactor (CCC), moving bed bioreactor (MBBR), and a bubble column reactor. A method is also provided where acetogenic bacteria are contacted with syngas in a growth fermentor section of a reactor vessel that is continuous with a main fermentor section of a reactor vessel.
    Type: Application
    Filed: May 15, 2012
    Publication date: January 3, 2013
    Inventors: Peter Simpson Bell, Ching-Whan Ko
  • Patent number: 8329459
    Abstract: A triphasic bioreactor for physico-chemically treating a gas is disclosed. The triphasic bioreactor comprises a reaction chamber with a liquid and biocatalysts in suspension in the liquid, for catalyzing a reaction between the gas and the liquid to obtain a treated gas and a solution containing a reaction product. A gas bubbling means is provided in the reaction chamber for bubbling the gas to be treated into the liquid thereby dissolving the gas into the liquid and increasing a pressure inside the reaction chamber. The bioreactor further comprises a liquid inlet in fluid communication with the reaction chamber for receiving the liquid and filling the reaction chamber, a liquid outlet in fluid communication with the reaction chamber for releasing the solution and a gas outlet in fluid communication with the reaction chamber to release the treated gas. The bioreactor further comprises a retention device to retain the biocatalysts in the reaction chamber.
    Type: Grant
    Filed: November 4, 2010
    Date of Patent: December 11, 2012
    Assignee: CO2 Solutions Inc.
    Inventors: Carmen Parent, Frédéric Dutil
  • Patent number: 8329460
    Abstract: A triphasic bioreactor for physico-chemically treating a gas is disclosed. The triphasic bioreactor comprises a reaction chamber with a liquid and biocatalysts in suspension in the liquid, for catalyzing a reaction between the gas and the liquid to obtain a treated gas and a solution containing a reaction product. A gas bubbling means is provided in the reaction chamber for bubbling the gas to be treated into the liquid thereby dissolving the gas into the liquid and increasing a pressure inside the reaction chamber. The bioreactor further comprises a liquid inlet in fluid communication with the reaction chamber for receiving the liquid and filling the reaction chamber, a liquid outlet in fluid communication with the reaction chamber for releasing the solution and a gas outlet in fluid communication with the reaction chamber to release the treated gas. The bioreactor further comprises a retention device to retain the biocatalysts in the reaction chamber.
    Type: Grant
    Filed: April 7, 2011
    Date of Patent: December 11, 2012
    Assignee: CO2 Solutions, Inc.
    Inventors: Carmen Parent, Frederic Dutil
  • Patent number: 8329458
    Abstract: A triphasic bioreactor for physico-chemically treating a gas is disclosed. The triphasic bioreactor comprises a reaction chamber with a liquid and biocatalysts in suspension in the liquid, for catalyzing a reaction between the gas and the liquid to obtain a treated gas and a solution containing a reaction product. A gas bubbling means is provided in the reaction chamber for bubbling the gas to be treated into the liquid thereby dissolving the gas into the liquid and increasing a pressure inside the reaction chamber. The bioreactor further comprises a liquid inlet in fluid communication with the reaction chamber for receiving the liquid and filling the reaction chamber, a liquid outlet in fluid communication with the reaction chamber for releasing the solution and a gas outlet in fluid communication with the reaction chamber to release the treated gas. The bioreactor further comprises a retention device to retain the biocatalysts in the reaction chamber.
    Type: Grant
    Filed: June 4, 2010
    Date of Patent: December 11, 2012
    Assignee: CO2 Solutions Inc.
    Inventors: Carmen Parent, Frédéric Dutil
  • Patent number: 8323958
    Abstract: The invention provides a device for growing genetically enhanced aquatic photoautotrophic organisms in a stable culture, causing said organisms to produce ethanol, and then separating, collecting, and removing the ethanol in situ.
    Type: Grant
    Filed: May 8, 2009
    Date of Patent: December 4, 2012
    Assignee: Algenol Biofuels Switzerland GmbH
    Inventors: R. Paul Woods, Edward Legere, Benjamin Moll, Edwin Malkiel
  • Publication number: 20120295333
    Abstract: Biological liquid substances in a disposable bioreactor or a disposable fermentor is enriched with a dissolved gas such as oxygen. The gas is provided to the liquid in meeting growth needs of biomass within the disposable vessel. The liquid is processed through a bubble forming element such as a supersonic mixer, a membrane sparger, or the like, to form a gas-liquid dispersion. The dispersion has a high interfacial surface area for facilitating gas dissolution in the liquid. Receptacles of the disposable vessel may be coupled (e.g., magnetically) to one or more processing devices (e.g., motors, pumps, gas sources) outside of the disposable vessel so that components within the disposable vessel are able to perform functions suitable for furthering reaction and/or fermentation processes.
    Type: Application
    Filed: May 18, 2012
    Publication date: November 22, 2012
    Inventor: Alan T. Cheng
  • Publication number: 20120295332
    Abstract: Oxygen is delivered into a liquid contained within a vessel. Oxygen-rich gas is introduced at an inner mixing region of a vessel toward an agitating element for dissolving some of the oxygen into the liquid. Air is introduced to the liquid at a location different from where the oxygen-rich gas is introduced, minimizing coalescing of bubbles of the oxygen-rich gas and air bubbles. Running the agitating element in the vessel may induce mixing of the liquid and improve oxygen dissolution. Movement of the air bubbles can also generate a mixing effect on the liquid, yet independent from mixing caused by the agitating element. In some embodiments, currents produced by the agitating element are asymmetric with respect to a vertical axis of the vessel. Air bubbles may also form an asymmetric configuration of bubbles about the vertical axis.
    Type: Application
    Filed: May 18, 2012
    Publication date: November 22, 2012
    Inventor: Alan T. Cheng
  • Patent number: 8227233
    Abstract: A method of making a molded part, including forming a liquid aggregate from a mixture of finely ground aggregate and a fluid. A mixture of a fungal inoculum and the liquid aggregate is formed. Nanoparticles are homogenously distributed throughout the mixture. The mixture is inserted into a mold cavity. Live mycelium is grown to fill the mold cavity. The live mycelium is heated to terminate further growth and develop a formed part.
    Type: Grant
    Filed: June 9, 2010
    Date of Patent: July 24, 2012
    Assignee: Ford Global Technologies, LLC
    Inventors: Raymond Edward Kalisz, Charles Alan Rocco
  • Patent number: 8183035
    Abstract: A method of manufacturing biological products in a single container from the growth of cells to purification of the product is performed in a flexible disposable bioreactor that uses only a compressed gas for mixing and gasification. A porous septum is used to create the gasification and mixing as well as to separate a chromatography media used to harvest and purify a biological product in the same container. The closed container can be used in any environment without the risk of contamination to the product or the risk of contamination of environment with the product. This allows large manufacturing of hazardous substances, drugs and vaccines anywhere at the lowest cost possible. Numerous applications can be found in counter-terrorism and biodefense operations as well in managing epidemic illnesses.
    Type: Grant
    Filed: September 7, 2011
    Date of Patent: May 22, 2012
    Assignee: Therapeutic Proteins International, LLC
    Inventor: Sarfaraz Niazi
  • Patent number: 8137959
    Abstract: The invention generally provides a cell culture vessel having at least one first zone and at least one second zone, wherein the first zone is a transfer zone for a culture medium which essentially contains no cells and the second zone is a cell culture zone. The invention further includes methods utilizing the cell culture vessel.
    Type: Grant
    Filed: April 4, 2008
    Date of Patent: March 20, 2012
    Assignee: Artelis S.A.
    Inventor: Jose Antonio Castillo Fernandez
  • Publication number: 20120052578
    Abstract: A gas-introduction system useful, for example, in biotechnology for supplying cells or microorganisms with oxygen. The gas-introduction system has a bubble column and a distributor. In the vessel there is situated a liquid medium which is to be supplied with gas. A vector is used as transport medium for the gas. The gas is introduced into the bubble column and here taken up by the vector. The vector is applied to the liquid surface via the distributor in droplet form, falls downward in the medium and releases to the medium some of the gas that has been taken up. At the bottom of the vessel is situated a collecting device in which the vector droplets coalesce and pass back into the bubble column. Disclosed are a bioreactor comprising the gas-introduction system and a method for introducing gas into a liquid medium, preferably an aqueous suspension containing cells or microorganisms.
    Type: Application
    Filed: January 13, 2010
    Publication date: March 1, 2012
    Applicant: BAYER TECHNOLOGY SERVICES GMBH
    Inventors: Joerg Kauling, Björn Frahm, Andre Pütz, Markus Schindler
  • Patent number: 8123199
    Abstract: A mixing bag for use in bioprocessing in which a fluid is received and agitated using an internal fluid-agitating element driven by an external motive device is disclosed. The bag may include an integral sparger and sensor receiver. Related methods are also disclosed.
    Type: Grant
    Filed: April 15, 2010
    Date of Patent: February 28, 2012
    Assignee: ATMI Packaging, Inc.
    Inventors: Alexandre N. Terentiev, Sergey Terentiev, Jeffery Lee Craig
  • Patent number: 8043848
    Abstract: In a bioreactor, a culture solution derived from a cultivation tank is separated into bacteria cells and filtrate by a bacteria cell filter. The filtrate is introduced from the bacteria cell filter into a micro-nano bubble generation tank where micro-nano-bubbles are mixed with the filtrate. The filtrate containing micro-nano-bubbles is returned to the cultivation tank to activate the microorganisms therein, so that a biological reaction time is reduced by the activated microorganisms.
    Type: Grant
    Filed: April 11, 2007
    Date of Patent: October 25, 2011
    Assignee: Sharp Kabushiki Kaisha
    Inventors: Kazuyuki Yamasaki, Kazuyuki Sakata, Kazumi Chuhjoh, Masaki Kataoka
  • Publication number: 20110256624
    Abstract: The invention relates to a bioreactor, to the use of the bioreactor for culturing microorganisms or cell cultures, and also to a method for culturing microorganisms or cell cultures.
    Type: Application
    Filed: December 8, 2009
    Publication date: October 20, 2011
    Applicant: BAYER TECHNOLOGY SERVICES GMBH
    Inventors: Marc Jenne, Björn Frahm, Joerg Kauling, Helmut Brod
  • Publication number: 20110229933
    Abstract: The present invention provides, in part, methods for recombinantly expressing proteins at a high level along with cell culture media for doing the same.
    Type: Application
    Filed: September 24, 2009
    Publication date: September 22, 2011
    Applicant: SAchering Corporaton
    Inventors: Rajesh Krishnan, Dennis Rendeiro, Sandeepa Sandadi
  • Publication number: 20110207218
    Abstract: A system for mixing a liquid solution or suspension includes a support housing bounding a compartment and a collapsible bag disposed within the compartment. A mixer is disposed within the collapsible bag while a sparger delivers a gas to the lower end of the collapsible bag. A gas outlet line extends from an upper end of the collapsible bag to a condenser assembly. A gas exhaust line and a fluid collection line both extend from the condenser assembly.
    Type: Application
    Filed: February 22, 2010
    Publication date: August 25, 2011
    Applicant: HYCLONE LABORATORIES, INC.
    Inventors: Clinton C. Staheli, Nephi D. Jones, Michael E. Goodwin
  • Publication number: 20110159590
    Abstract: A device, system and method for axenically culturing and harvesting cells and/or tissues, including bioreactors and fermentors. The device is preferably disposable but nevertheless may be used continuously for a plurality of consecutive culturing/harvesting cycles prior to disposal of same. This invention also relates to batteries of such devices which may be used for large-scale production of cells and tissues. According to preferred embodiments of the present invention, the present invention is adapted for use with plant cell culture.
    Type: Application
    Filed: January 18, 2011
    Publication date: June 30, 2011
    Applicant: Protalix Ltd.
    Inventor: Yoseph SHAALTIEL
  • Publication number: 20110136212
    Abstract: Systems, methods and media for carbonation of fluid in an algae cultivation pond via the use of backward-facing steps are disclosed. In a first aspect, a method for initiating carbonation of a fluid flow in an algae cultivation pond is disclosed. Fluid flow is initiated over a backward-facing step in the algae cultivation pond. A backflow region is generated in the fluid flow downstream of the backward-facing step. Carbon dioxide is provided to the backflow region.
    Type: Application
    Filed: December 4, 2009
    Publication date: June 9, 2011
    Inventors: Mehran Parsheh, David Rice, Guido Radaelli
  • Publication number: 20110081712
    Abstract: A method and apparatus for extracting CO2 from air comprising an anion exchange material formed in a matrix exposed to a flow of the air, and for delivering that extracted CO2 to controlled environments. The present invention contemplates the extraction of CO2 from air using conventional extraction methods or by using one of the extraction methods disclosed; e.g., humidity swing or electro dialysis. The present invention also provides delivery of the CO2 to greenhouses where increased levels of CO2 will improve conditions for growth. Alternatively, the CO2 is fed to an algae culture.
    Type: Application
    Filed: October 13, 2010
    Publication date: April 7, 2011
    Inventors: Allen B. Wright, Klaus S. Lackner, Ursula Ginster
  • Patent number: 7888111
    Abstract: An object of the present invention is to provide a cell culture vessel which is simple in structure and easy to handle, and is capable of preventing damage to the cells when separated, promoting transport of nutrients and excretion of effete matter, and elevating the culturing efficiency improving effect by the structural features. In order to attain the above object, there is provided a cell culture vessel including a culture section provided with a plurality of projections having an equivalent diameter smaller than the cells to be cultured and the culture section side walls enclosing the culture section, wherein the distance between an arbitrary position on the culture section/side wall boundary line and the nearest projection is smaller than the diameter of the cells to be cultured. The effect of the projections in the vessel given to the cultured cells is enhanced.
    Type: Grant
    Filed: November 9, 2009
    Date of Patent: February 15, 2011
    Assignee: Hitachi, Ltd.
    Inventors: Kosuke Kuwabara, Akihiro Miyauchi, Norihito Kuno
  • Patent number: 7875448
    Abstract: The present invention is in the field of cell bioreactors, and specifically in the field of disposable bioreactors.
    Type: Grant
    Filed: December 13, 2004
    Date of Patent: January 25, 2011
    Assignee: Single Use BRX, LLC
    Inventor: James Francis Furey
  • Publication number: 20100316446
    Abstract: Novel bioreactor systems, adapted to provide continuous batch incubation of microbes, especially bacteria, are provided. Bioreactor bags are preloaded with inert microbes prior to being shipped to the user. The inert microbes may be substantially dry or in other inert forms, and are stored in soluble containers within the bioreactor bags. Multiple sterile, preloaded, disposable bioreactor bags are used in a self-contained housing. The bags are automatically incubated in the housing and dispensed, preferably into an irrigation system. The system provides serial batch production of useful microbes for a week or more, without further supervision or intervention. Methods of manufacture, methods of use, methods of sales, and methods of farming are also provided.
    Type: Application
    Filed: February 25, 2010
    Publication date: December 16, 2010
    Inventor: Larry Runyon
  • Patent number: 7850365
    Abstract: A device for circulating a liquid media in a vessel includes a gas infusion tube having a leading end immersed in the liquid media and a trailing end in fluid communication with a remote air pump. A diffuser is secured to the leading end of the gas infusion tube. The lumen of an outer tube receives the gas infusion tube and the diffuser. The outer tube has a leading end disposed in spaced relation to a bottom wall of the vessel and has a trailing end substantially flush with a level of the liquid media. A plurality of circumferentially spaced openings are formed in the trailing end of the outer tube or in a cap secured to the trailing end. Bubbles are emitted from the diffuser, entraining the liquid media so that it follows a toroidal path of travel that maximizes cell growth.
    Type: Grant
    Filed: July 16, 2009
    Date of Patent: December 14, 2010
    Inventors: Alan Maxwell Much, Carolyn Much, legal representative
  • Publication number: 20100311146
    Abstract: A mixing vessel (10) for containing a liquid, comprises a chamber having a lower chamber portion and an upper chamber portion wider than the lower portion, gas inlet means (14) for supplying gas to the lower portion, liquid circulation inducing means (17) and an inclined abutment (24), such that, when gas is supplied via the gas inlet means gas in the form of bubbles initially rises substantially vertically in liquid and is redirected in a substantially horizontal direction by the abutment.
    Type: Application
    Filed: June 11, 2008
    Publication date: December 9, 2010
    Applicant: Cellexus Limited
    Inventor: Kevin Auton
  • Publication number: 20100248344
    Abstract: A methanogenic reactor for the production of methane, cellular biomass and other useful products for use in the manufacturing of specialty chemicals. The methanogenic reactor includes a bottom wall, perimeter wall, and top wall defining an interior space environmentally separable from an exterior space outside of the reactor vessel for holding a methanogenic culture and growth media. The reactor also includes at least one sparger positioned substantially within the interior space for facilitating the direction of an input gas stream into the reactor to be brought into contact with the methanogenic culture. The reactor also includes an output material stream port for releasing an output material stream created at least in part by the methanogenic culture.
    Type: Application
    Filed: March 27, 2009
    Publication date: September 30, 2010
    Inventors: Bruce G. Schroder, Russell A. Pohl
  • Publication number: 20100193433
    Abstract: A bioreactor for treating a liquid comprises a container having a vertical wall portion with at least one inlet port and at least one outlet port. The bioreactor also comprises a media support platform securely disposed inside the container at a position below the inlet and outlet ports, and substantially permeable to liquid. The bioreactor comprises at least one cylindrical chamber inside the container at a position above the media support platform. The bioreactor also comprises a plurality of media units topically disposed on the media support platform. The bioreactor comprises a recirculation/aeration system secured at a bottom end of the cylindrical chamber. The recirculation/aeration system has a submersible pump device that can be programmed to pump liquid at variable speeds and has a liquid intake portion having perforations, an air intake tube, and an eductor device. The air intake tube protrudes through a top port of the container.
    Type: Application
    Filed: May 23, 2008
    Publication date: August 5, 2010
    Applicant: TVT US CORP.
    Inventors: Volker A. Hausin, Daniel J. LaGassee
  • Publication number: 20100136673
    Abstract: A device, system and method for axenically culturing and harvesting cells and/or tissues, including bioreactors and fermentors. The device is preferably disposable but nevertheless may be used continuously for a plurality of consecutive culturing/harvesting cycles prior to disposal of same. This invention also relates to batteries of such devices which may be used for large-scale production of cells and tissues. According to preferred embodiments of the present invention, the present invention is adapted for use with plant cell culture.
    Type: Application
    Filed: January 21, 2010
    Publication date: June 3, 2010
    Applicant: Protalix Ltd.
    Inventor: Yoseph SHAALTIEL
  • Patent number: 7727758
    Abstract: The present invention relates to an extraction and brewing machine having a tank, a fan assembly, a base aerator, an extractor head, and an extraction assembly. The extraction assembly has a container for being submerged in liquid held within the tank, and an extraction aerator. The container is comprised of a dome and a filter. The dome defines an interior region for vigorous mixing of air, compost and water. The filter can be a mesh bag. The extraction aerator can have openings near the bottom of the bag. The flow of air though the filter causes a current of liquid, air and microorganisms to exit the chamber. The base aerator aerates all the liquid in the tank. The microorganisms in the tea continue to grow and multiply in the tank after extraction from the chamber, and no particles pass the filter that could jam a spray nozzle.
    Type: Grant
    Filed: March 16, 2006
    Date of Patent: June 1, 2010
    Inventor: Robert Posthuma