Abstract: A bioreactor for treating a liquid comprises a container having a vertical wall portion with at least one inlet port and at least one outlet port. The bioreactor also comprises a media support platform securely disposed inside the container at a position below the inlet and outlet ports, and substantially permeable to liquid. The bioreactor comprises at least one cylindrical chamber inside the container at a position above the media support platform. The bioreactor also comprises a plurality of media units topically disposed on the media support platform. The bioreactor comprises a recirculation/aeration system secured at a bottom end of the cylindrical chamber. The recirculation/aeration system has a submersible pump device that can be programmed to pump liquid at variable speeds and has a liquid intake portion having perforations, an air intake tube, and an eductor device. The air intake tube protrudes through a top port of the container.

Abstract: A device, system and method for axenically culturing and harvesting cells and/or tissues, including bioreactors and fermentors. The device is preferably disposable but nevertheless may be used continuously for a plurality of consecutive culturing/harvesting cycles prior to disposal of same. This invention also relates to batteries of such devices which may be used for large-scale production of cells and tissues. According to preferred embodiments of the present invention, the present invention is adapted for use with plant cell culture.

Abstract: The present invention relates to an extraction and brewing machine having a tank, a fan assembly, a base aerator, an extractor head, and an extraction assembly. The extraction assembly has a container for being submerged in liquid held within the tank, and an extraction aerator. The container is comprised of a dome and a filter. The dome defines an interior region for vigorous mixing of air, compost and water. The filter can be a mesh bag. The extraction aerator can have openings near the bottom of the bag. The flow of air though the filter causes a current of liquid, air and microorganisms to exit the chamber. The base aerator aerates all the liquid in the tank. The microorganisms in the tea continue to grow and multiply in the tank after extraction from the chamber, and no particles pass the filter that could jam a spray nozzle.

Abstract: A pneumatic bioreactor having a containment vessel which includes a semi-cylindrical concavity defined by the vessel bottom. A mixing apparatus includes a rotational mixer rotatably mounted within the containment vessel about a horizontal axis. The rotational mixer has buoyancy-driven mixing cavities which are fed by a gas supply beneath the rotational mixer. The mixing apparatus extends into the semi-cylindrical concavity to substantially fill that concavity. The rotational mixer is divided into two wheels with outer paddles extending axially outwardly and inner paddles extending axially inwardly on either side of each wheel. Blades between the outer and inner paddles form impellers in the wheels to induce axial flow through the wheels in opposite directions. Stationary baffles fixed relative to the containment vessel are inclined toward the rotational mixer in the direction of rotation.

Abstract: An object of the present invention is to provide a cell culture vessel which is simple in structure and easy to handle, and is capable of preventing damage to the cells when separated, promoting transport of nutrients and excretion of effete matter, and elevating the culturing efficiency improving effect by the structural features. In order to attain the above object, there is provided a cell culture vessel including a culture section provided with a plurality of projections having an equivalent diameter smaller than the cells to be cultured and the culture section side walls enclosing the culture section, wherein the distance between an arbitrary position on the culture section/side wall boundary line and the nearest projection is smaller than the diameter of the cells to be cultured. The effect of the projections in the vessel given to the cultured cells is enhanced.

Abstract: A triphasic bioreactor for physico-chemically treating a gas is disclosed. The triphasic bioreactor comprises a reaction chamber with a liquid and biocatalysts in suspension in the liquid, for catalyzing a reaction between the gas and the liquid to obtain a treated gas and a solution containing a reaction product. A gas bubbling means is provided in the reaction chamber for bubbling the gas to be treated into the liquid thereby dissolving the gas into the liquid and increasing a pressure inside the reaction chamber. The bioreactor further comprises a liquid inlet in fluid communication with the reaction chamber for receiving the liquid and filling the reaction chamber, a liquid outlet in fluid communication with the reaction chamber for releasing the solution and a gas outlet in fluid communication with the reaction chamber to release the treated gas. The bioreactor further comprises a retention device to retain the biocatalysts in the reaction chamber.

Abstract: Accordingly, in one embodiment of the invention, a bioreactor system is presented and includes a disposable container for housing biomaterials for processing, the disposable container including at least one input port, at least one exhaust port, at least one harvest port, and the integrity of the sterile environment is protected with sterile filters attached to all external open ports a structure for supporting the disposable container, one or more sensors for sensing one or more parameters of the biomaterials in the container, a heater for heating the contents of the container, the heater having a thermostat and mixing system arranged with the system such that biomaterials contained in the disposable container are mixed.

Abstract: The present invention relates to a device for the aeration of liquid media by means of tube aeration, special tube modules contained therein and the use of a device for the aeration of liquid media.

Abstract: The invention relates to a bioreactor (1) for the purification of waters, said reactor comprising a cross-sectionally essentially circular or elliptical tank section (2) provided with inlet means (5) for water to be purified and outlet means (6) for purified water. The tank holds thereinside carrier material (3) on which a biofilm may develop. The tank is further provided with means (4) for supplying a fluid which contains a reaction gas required by the purification process, such that the water to be purified develops gas bubbles containing a reaction gas. The tank section is adapted to be essentially full of water during the purification process. The fluid supply means (4) are disposed on the tank wall and the reactor comprises control means for operating the fluid supply means in such a way that a spinning motion of the carrier, the water, and at least some of said reaction-gas bearing bubbles is effected thereby around a rotation centerline passing essentially through the tank's cross-sectional center.

Abstract: A mixing vessel (10) for containing a liquid, comprises a chamber having a lower chamber portion and an upper chamber portion wider than the lower portion, gas inlet means (14) for supplying gas to the lower portion and means for redirecting rising gas (24), such that, in use, rising gas in the form of bubbles, initially rises substantially vertically and is redirected in a substantially horizontal direction by the means for redirecting rising gas.

Abstract: A continuous process for production of solvents, particularly acetone-butanol-ethanol (ABE) using fermentation of solventogenic microorganisms and gas stripping is provided. The solventogenic microorganisms are inoculated in a nutrient medium containing assimilable carbohydrates (substrate) and optional other additives. Control of the solventogenic microorganism concentration in the fermentor (cell concentration) and the assimilable carbohydrate concentration in the fermentor, along with removal of solvents formed results in a continuous process for production of solvents.

Abstract: A culturing apparatus for photosynthesis microorganism provided by the present invention comprises a outer vessel, inner vessel, culture solution circulating portion and a heat transfer medium feeding portion. Culture solution containing photosynthesis microorganism is put into the outer vessel which is transparent and configured like a cylinder extending in a predetermined axis-direction. The inner vessel is disposed in the outer vessel, being configured like a cylinder extending in the above axis-direction. The culture solution circulating portion draws culture solution from one side along the axis-direction in the outer vessel, supplying that to the other side of the axis-direction. The heat transfer medium feeding portion supplies heat transfer medium into the inner vessel.

Abstract: A moving bed bioreactor (MBBR) produces liquid products from a gaseous substrate of CO and/or CO2/H2 using a biomass that grows on the surface of carrier suspended in a fermentation broth into which the gaseous substrate is at least partially dissolved. The injection devices include gas spargers and preferably a high efficiency gas transfer system such as jet or slot aerator/gas transfer devices. The gas injection device creates eddy currents in the surrounding liquid for thoroughly mixing the fermentation broth in a fermentation vessel. Gas bubbles from the gas delivery device rise through the liquid surface and provide additional mixing and gas dissolution. The motion of gas and liquid keeps the biomass carrier moving can also provide sufficient shear so as to maintain the biofilm thickness on the biomass carrier media in the desirable range.

Abstract: A scalable photobioreactor system for efficient production of photosynthetic microorganisms such as microalgae and cyanobacteria is described. In various embodiments, this system may include the use of extended surface area to reduce light intensity and increase photosynthetic efficiency, an external water basin to provide structure and thermal regulation at low cost, flexible plastic or composite panels that are joined together make triangular or other shapes in cross-section when partially submerged in water, use of positive gas buoyancy and pressure to maintain the structural integrity of the photobioreactor chambers and use of structure to optimize distribution of diffuse light. Other embodiments concern air tubes comprised of plastic film at the bottom of each photobioreactor chamber to provide sparging air bubbles to the chamber. The photobioreactor system design also comprises gas exchange, temperature control, air pumping, liquid pumping, filtration, media recycling and harvesting methods.

Abstract: A bioreactor formed of a material such as glass or plastic is provided having a first closed wall spaced apart from a second closed wall. At least a portion of the height of the first closed wall extends toward the center of the bioreactor so that gas introduced at the bioreactor bottom contacts the first closed wall prior to contacting the top surface of reactants in the bioreactor.

Abstract: A mixing bag for use in bioprocessing in which a fluid is received and agitated using an internal fluid-agitating element driven by an external motive device is disclosed. The bag may include an integral sparger and sensor receiver. Related methods are also disclosed.

Abstract: A bioreactor formed of a flexible material is provided having a constant aspect ratio and an adjustable length.

Abstract: A bioreactor formed of a flexible material is provided having a constant aspect ratio and an adjustable length. The bioreactor is rendered self standing by a support means secured to the bioreactor.

Abstract: The invention relates to an in home apparatus for simultaneously suspending, aerating, agitating, extracting and entraining a compost water nutrient culture. A beaker contains the compost nutrient water culture. A perforated elastic membrane transversely covers the open beaker bottom and is retained and compression sealed by a holder. The holder mates with a recess provided by upper portions of a base. The base contains a diaphragm air pump supplying air through the perforated elastic membrane releasing air bubbles into the compost, water nutrient culture in the beaker. Pulsating air supply from the diaphragm air pump vibrates the perforated elastic membrane which in combination with the sparging of air bubbles suspends the compost nutrient water culture preparing a high quality compost tea. The beaker, holder, and base easily engage and disengage for the proper cleaning and convenient in home preparation of compost tea.

Abstract: The present invention relates to a bioreactor for sewage and wastewater treatment, which is capable of agitating and supplying oxygen simultaneously. A bioreactor of the present invention, comprises a culture drum in which microorganisms are cultured; a supplier for supplying a culture solution, and organic and inorganic media to the culture drum, the supplier including a supply tube connected to an upper side of the culture drum, and a pump connected to the supply tube for supplying the culture solution to the culture drum; an agitator for agitating the culture solution by spraying air into the culture solution in the culture drum, the air being supplied from an air blower; and a thermostatic controller for controlling a temperature of the culture solution and disinfecting the interior of the culture drum.

Abstract: The bioreactor is for use in performing biological and/or biochemical reactions and includes a vessel, an agitator, a reaction assembly, and a harvesting outlet. The vessel of the bioreactor includes several ports including a mixing port, a reaction port, and a harvesting port. The agitator extends through the mixing port into the vessel while the harvesting outlet extends through the harvesting port and permits the withdrawal of reaction medium to another vessel. The reaction assembly extends through the reaction port into the vessel and has multiple components including a gas conduit adapted to introduce gas into a reaction medium in the vessel, a sampling device adapted to remove a portion of the reaction medium from the vessel without contamination of the remaining reaction medium, and an introduction conduit permitting the introduction of at least the reaction medium into the vessel.

Abstract: In a bioreactor, a culture solution derived from a cultivation tank is separated into bacteria cells and filtrate by a bacteria cell filter. The filtrate is introduced from the bacteria cell filter into a micro-nano bubble generation tank where micro-nano-bubbles are mixed with the filtrate. The filtrate containing micro-nano-bubbles is returned to the cultivation tank to activate the microorganisms therein, so that a biological reaction time is reduced by the activated microorganisms.

Abstract: An apparatus for forcing airflow through (i.e., aerating) a particulate material consists of a planar bearing surface and a ventilation conduit underlying the planar bearing surface. A plurality of delivery conduits extend between the ventilation conduit and the planar bearing surface with a mouth of the delivery conduits being near the planar bearing surface. A grate, defining a plurality of openings, covers the mouth of each delivery conduit. Each grate has a top surface substantially parallel to the planar bearing surface and positioned at or no more than a select distance below the planar bearing surface. This select distance is close enough to the planar bearing surface to prevent the accumulation of particulate material over the grate as the particulate material is scraped from the planar bearing surface with an implement having a linear edge abutting the planar bearing surface.

Abstract: The present invention relates to a device for cultivating plant or animal tissue. The inventive device comprises a fermenter container, a supply device for liquid nutrients and gases, devices for discharging used nutritive liquids and gases and solid carrier plates for the plant tissues which are permeable to said liquid nutrients and gases and are arranged within the fermenter container in a stationary manner. According to the invention, the fermenter container comprises a dimensionally stable lid component that carries the various supply and discharge lines as well as the carrier for the cells and comprises a pot- or bag-shaped plastic hose. The opening of said hose is sealed against the lid and is fixed.

Abstract: The present invention describes all apparatus for making a foliar spray from a liquid and a particulate matter. The apparatus consists of a non-porous liquid receptacle, a basket for holding the particulate matter, intake means for transporting air from a blower to a manifold means for aerating the liquid and the particulate matter, and a blower for supplying the air. The liquid receptacle is typically a substantially horizontal, rectangular tank having a flat bottom and the manifold means includes a selected number of diffuser arms, the number of diffuser arms selected to cover a substantial portion of the area of the bottom surface of the liquid receptacle and is selectively proportionate to the volume of the receptacle. The manifold means may be easily removed from the liquid receptacle by means of a quick-disconnect coupler for ease of cleaning the liquid receptacle once the foliar liquid is removed from the liquid receptacle via outlet means.

Abstract: Apparatuses and methods for anaerobic digestion of high-solids waste are provided. The methods may include and the apparatuses may be used for moving the solid waste in a corkscrew-like fashion through a closed container. The method may further include moving the high-solids waste into contact with a heating device to facilitate the corkscrew-like movement. Other methods and apparatuses may use at least one of a partition and a conduit from which liquid or gas is discharged.

Abstract: A fermentation apparatus is constructed to produce a known and repeatable amount of untainted fermentation product using multiple fermentation vessels. To facilitate further processing compatible with other product processing steps, the fermentation apparatus has an array of sample vessels arranged in a container frame. The container frame is configured to hold the sample vessels during fermentation and to transport the vessel array to or from another processing station. Corresponding to the number of sample vessels in the sample vessel array, a cannula array is configured such that each cannula may be placed inside a sample vessel. The cannula array is attached to a gas distributor that delivers oxygen and/or one or more other gases from a gas source through the cannula into the sample vessel. Because the fermentation volume for each individual sample vessel is smaller than a bulk fermentation apparatus, the fermentation product yields are predictable and cell growth rates can be effectively optimized.

Abstract: A method for deodorizing a storage tank having mercaptan residue disposed therein includes the steps of delivering a cleaning material to the storage tank, and absorbing the mercaptan residue from the walls of the tank into the cleaning material. The cleaning material having absorbed the mercaptan is transferred to a recovery vessel. The mercaptan is continuously sparged from the cleaning material with natural gas for continuously and repeated using the cleaning material.

Abstract: A compost tea system uses a water-holding tank for containing process water. Compost is immersed into the water in the tank in baskets defining a filter media. Air is pumped into membrane disk diffuser modules in the tank and the liquid is sparged with fine bubbles to thoroughly agitate the liquid, extract nutrients and microorganisms from the compost, and to ensure a high concentration of dissolved oxygen in the liquid. The high level of oxygen selects for desired aerobic organisms to produce a rich compost tea. The finished tea is drained out of the tank and the tank and its components are easily cleaned and sanitized.

Abstract: The present invention relates to a bioreactor for sewage and wastewater treatment, which is capable of agitating and supplying oxygen simultaneously. A bioreactor of the present invention, comprises a culture drum in which microorganisms are cultured; a supplier for supplying a culture solution, and organic and inorganic media to the culture drum, the supplier including a supply tube connected to an upper side of the culture drum, and a pump connected to the supply tube for supplying the culture solution to the culture drum; an agitator for agitating the culture solution by spraying air into the culture solution in the culture drum, the air being supplied from an air blower; and a thermostatic controller for controlling a temperature of the culture solution and disinfecting the interior of the culture drum.

Abstract: The improved compost tea producing apparatus and methods of the present invention are directed to aeration means which substantially increases the count of beneficial organisms in liquid compost tea compositions. Such aeration apparatus of the present invention includes an air blower having an air provision capacity of at least approximately 350-500 ft/min. An air conduit is connected to the output port of the air blower, with such conduit having a generally vertical portion and such vertical portion having an effluent opening. An air diffuser tube is attached to the effluent opening of the vertical portion of the air conduit, with such air diffuser tube having a plurality of air bubbling apparatus as disposed along the length thereof for transmitting air into a volume of liquid compost tea.

Abstract: A triphasic bioreactor for physico-chemically treating a gas is disclosed. The triphasic bioreactor comprises a reaction chamber with a liquid and biocatalysts in suspension in the liquid, for catalyzing a reaction between the gas and the liquid to obtain a treated gas and a solution containing a reaction product. A gas bubbling means is provided in the reaction chamber for bubbling the gas to be treated into the liquid thereby dissolving the gas into the liquid and increasing a pressure inside the reaction chamber. The bioreactor further comprises a liquid inlet in fluid communication with the reaction chamber for receiving the liquid and filling the reaction chamber, a liquid outlet in fluid communication with the reaction chamber for releasing the solution and a gas outlet in fluid communication with the reaction chamber to release the treated gas. The bioreactor further comprises a retention device to retain the biocatalysts in the reaction chamber.

Abstract: A compost tea system uses a water-holding tank for containing process water. Compost is immersed into the water in the tank in baskets defining a filter media. Air is pumped into membrane disk diffuser modules in the tank and the liquid is sparged with fine bubbles to thoroughly agitate the liquid, extract nutrients and microorganisms from the compost, and to ensure a high concentration of dissolved oxygen in the liquid. The high level of oxygen selects for desired aerobic organisms to produce a rich compost tea. The finished tea is drained out of the tank and the tank and its components are easily cleaned and sanitized.

Abstract: A disposable device and method for axenically culturing and harvesting cells and/or tissue in consecutive cycles. The device consist of a sterilizable transparent and/or translucent disposable container which may be at least partially filled with a suitable sterile biological cell and/or tissue culture medium and/or axenic inoculant and/or sterile air and/or required other sterile additives. The container has means for removing excess air and/or waste gases therefrom, and means for introducing the inoculant and/or culture medium and/or additives therein. The device also has at least one air inlet, and preferably a plurality thereof, for introducing sterile air into the device. The device is characterised by having a reusable harvesting means comprising suitable flow control means for enabling harvesting of at least a portion of the medium containing cells and/or tissue when desired, thereby enabling the device to be used continuously for at least one subsequent consecutive culturing/harvesting cycle.

Abstract: A disposable bioreactor for culturing microorganisms and cells is provided. The bioreactor is suitable for use by individuals not skilled in microbiology or aseptic technique. It is constructed of flexible or semi-flexible waterproof sheets to form a container designed to provided mixing and gas exchange to microorganisms cultured therein. Mixing and gas exchanged are achieved by bubbling gas through the culture, either from a single locus at the lowermost apex of a container having a wedge-shaped or rounded bottom, or from multiple loci across a flat-bottomed container. Also provided is a kit for culturing a selected microorganism, preferably an entomopathogenic nematode or other organism useful as a biopesticide.

Abstract: A disposable device and method for axenically culturing and harvesting cells and/or tissue in consecutive cycles. The device consists of a sterilisable disposable container which may be partially filled with a suitable sterile biological cell and/or tissue culture medium. The container has means for removing excess air and/or waste gases therefrom, and means for introducing inoculant and/or culture medium and/or additives therein. A reusable harvesting means enables harvesting of at least a portion of the medium containing cells and/or tissue when desired, thereby enabling the device to be used continuously for at least one subsequent consecutive culturing/harvesting cycle. The portion of medium containing cells and/or tissue remaining from a previously harvested cycle may serve as inoculant for a next culture and harvest cycle.

Abstract: This invention relates to a culture device of a domed shape, a conical shape, or a cylindrical shape of a closed type used for culture of microalgae, and a gas discharge device set so as to be movable in the culture device. The culture device is basically composed of a transparent inside member (a semispherical dome, a conical peripheral wall, or a cylindrical peripheral wall), a transparent outside member, and a bottom portion connecting the lower ends of the two members, a cylindrical opening portion is provided at the top part of the outside member, and a gas introducing member and a discharging member of a culture solution are provided in the bottom portion. The gas discharge device is basically composed of two opposed rectangular base plates, a bubble guide member, and a discharge nozzle. The culture solution can be agitated without mechanical agitation and the culture can be carried out in high concentrations.

Abstract: The invention is a system and a method for bioremediating hydrocarbon-contaminated groundwater. The method comprises contacting the hydrocarbon-contaminated groundwater with a source of microbial nutrients and then aerating the nutriated water with air or oxygen, the aeration being conducted under substantially bubble-free conditions.