Including Perfusion Means Patents (Class 435/297.2)
  • Patent number: 10947489
    Abstract: A bioreactor (1; 2; 3) includes a receiving container (10) for receiving a disposable bag (44). A bottom surface (11) of the receiving container (10) is designed to support the disposable bag (44) in the receiving container (10). An outlet opening (5) is formed in the bottom surface (11) and tapers down with respect to the ground for receiving a drain port (45) of the disposable bag (44). The drain port is complementary to the outlet opening (5).
    Type: Grant
    Filed: March 8, 2016
    Date of Patent: March 16, 2021
    Inventors: Bernward Husemann, Simon Topp-Manske, Bjoern Nickel, Marco Leupold
  • Patent number: 10934517
    Abstract: A laboratory container, in particular a cell culture container, includes a container volume which is surrounded by a container body and which can be accessed from the outside through a container opening of the container body. The container has a fluid line which communicates with the container volume and which comprises a valve arrangement. The fluid line is designed to conduct fluid in order to discharge and/or introduce fluid out of and/or into the container volume. The fluid line can be selectively released in order to conduct a fluid or blocked by the valve arrangement, and the container additionally has a gas balancing opening, through which gas can be introduced into or discharged out of the container volume in counterflow to a possible fluid flow in the fluid line. The gas compensating opening is surrounded by a channel wall, said line channel running from the gas balancing opening into the container volume and opening into said container volume.
    Type: Grant
    Filed: May 22, 2015
    Date of Patent: March 2, 2021
    Assignee: HAMILTON BONADUZ AG
    Inventors: Thomas J├Ąger, Tobias Seiler, Carsten Etzold
  • Patent number: 10731119
    Abstract: The invention is directed to a method for the in vitro production of arrangements of cell layers in which a first well (2.1) which is closed off from its environment except for a first inlet opening (4.1) and a first outlet opening (5.1) and which has as a first cell substrate (6.1) a first wall (2.1.1) and as a second cell substrate (6.2) an opposite, second wall (2.1.2) which is separated from the first wall (2.1.1) by a first gap, a free surface of a cell substrate (6.1, 6.2) to be colonized with cells is oriented orthogonal to the Earth's gravitational force, and cells (9) are adhered to the cell substrate (6.1, 6.2) to be colonized. The invention is further directed to a method of maintaining the biological functionalities of the cell layers and semi-finished products of a device for the in vitro production and culturing of cell layers and a method for the production of the device.
    Type: Grant
    Filed: May 7, 2015
    Date of Patent: August 4, 2020
    Assignees: Universitaetsklinikum Jena, Microfluidic ChipShop GmbH
    Inventors: Alexander Mosig, Knut Rennert
  • Patent number: 10717961
    Abstract: A cell culture system having a cell culture vessel, a composition controlling fluid storage vessel, a culture fluid composition controlling means having an inlet and an outlet for a cell culture fluid, an inlet-connected fluid feeding circuit from the cell culture vessel to the inlet of the culture fluid composition controlling means, an outlet-connected fluid feeding circuit from the cell culture vessel to the outlet of the culture fluid composition controlling means, a means which perfuses the cell culture fluid from the inlet-connected fluid feeding circuit to the outlet-connected fluid feeding circuit through the culture fluid composition controlling means, and a means which controls the amount of fluid in the cell culture vessel, in which compositions of the cell culture fluid in the cell culture vessel and compositions of the composition controlling fluid in the composition controlling fluid storage vessel can be controlled in a continuous manner.
    Type: Grant
    Filed: April 24, 2013
    Date of Patent: July 21, 2020
    Assignees: ASAHI KASEI KABUSHIKI KAISHA, TOKYO WOMEN'S MEDICAL UNIVERSITY
    Inventors: Ushio Iwamoto, Michi Sato, Kanako Konishi, Katsuhisa Matsuura, Tatsuya Shimizu, Teruo Okano
  • Patent number: 10702615
    Abstract: An apparatus for sterilization of objects or materials comprises an enclosure for containing a gaseous atmosphere; an acoustically transparent platform located within the enclosure for supporting the objects or materials to be sterilized; one or more non-contact ultrasound transducers positioned within the enclosure and spaced from the platform; and an electric power amplifier for exciting the one or more transducers.
    Type: Grant
    Filed: October 18, 2017
    Date of Patent: July 7, 2020
    Assignee: The Ultran Group, Inc.
    Inventors: Kashyap Patel, Mahesh Bhardwaj, Michael Whetzel, Anuj Bhardwaj, Mikel Langron
  • Patent number: 10662401
    Abstract: The present invention provides a production module for large-scale production of cells and/or cell-derived products such as antibodies or virus; a production suite comprising a plurality of functionally connected production modules of the invention; and a method for large-scale production of cells and/or cell-derived products using the production modules and/or production suites of the invention.
    Type: Grant
    Filed: January 31, 2018
    Date of Patent: May 26, 2020
    Assignee: BIOVEST INTERNATIONAL, INC.
    Inventors: Mark Hirschel, Robert J. Wojciechowski, Kim Arneson
  • Patent number: 10519414
    Abstract: Disclosed is a method of continuously culturing cells, including culturing the cells by injecting a culture medium into an internal compartment of a sealed culture vessel and then inoculating the culture medium with the cells, detaching the adhered cells when the density of the cells cultured in the compartment of the culture vessel is a reference value or more, and obtaining the cells detached from the culture vessel, which is maintained in a sealed state.
    Type: Grant
    Filed: October 16, 2014
    Date of Patent: December 31, 2019
    Assignee: MEDIKAN INC
    Inventor: Hee Young Lee
  • Patent number: 10479669
    Abstract: A beverage-making apparatus includes a container including a container body that includes heat-insulating material and has an opening that provides access to an interior of the container body. The container further includes: a container cover configured to open and close the opening of the container body, and at least three channels connected to the container and that are communicative with the interior of the container body. The at least three channels include at least one first channel and a second channel that are communicative with a beverage ingredient pack mounted in the interior of the container body, the beverage ingredient pack configured to hold first beverage-making ingredients. The container further includes a third channel that is communicative with a space formed in the interior of the container body between a wall of the container body and the beverage ingredient pack mounted in the interior the container body.
    Type: Grant
    Filed: May 18, 2017
    Date of Patent: November 19, 2019
    Assignee: LG Electronics Inc.
    Inventors: Heeyeon Kim, Sunyoung Park, Chungook Chong
  • Patent number: 10301584
    Abstract: A perfusion bioreactor chamber for engineering a broad spectrum of tissues. The bioreactor allows controlled distribution of fluid through or around scaffolding materials of various shapes, structures and topologies during prolonged periods of cultivation.
    Type: Grant
    Filed: April 17, 2014
    Date of Patent: May 28, 2019
    Assignee: THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK
    Inventors: Gordana Vunjak-Novakovic, Sarindr Bhumiratana, Keith Yeager
  • Patent number: 10280391
    Abstract: A recipient for cell cultivation having an inner compartment adapted for cell growth, in one embodiment, an outer tubular wall extends in a longitudinal direction and delimits an outer boundary of the inner compartment in a radial direction. First and second ends delimit the inner compartment at the first respectively the second outer end of the outer tubular wall. A fixed packing in the inner compartment comprises a packing, such as a fiber matrix. Additional embodiments and related methods are also disclosed.
    Type: Grant
    Filed: December 11, 2013
    Date of Patent: May 7, 2019
    Assignee: PALL TECHNOLOGY UK LIMITED
    Inventors: Jean-Christophe Drugmand, Jose Antonio Castillo Gonzalez, Vishwas Pethe, Matthew Kremer
  • Patent number: 10245368
    Abstract: The present invention relates to the field of bio-artificial liver technology. Particularly, the invention provides an integrated hybrid bio-artificial liver support system that provides the features of artificial liver assist devices with that of bio-artificial liver support systems.
    Type: Grant
    Filed: July 29, 2016
    Date of Patent: April 2, 2019
    Assignee: INDIAN INSTITUTE OF TECHNOLOGY, KANPUR
    Inventors: Ashok Kumar, Apeksha Damania
  • Patent number: 10214715
    Abstract: A bioreactor system for stem cell expansion has a fluid-filled reactor chamber in which stem cells are cultured. The culture compartment of the reactor chamber is transversed by a perfusion core with multiple niche-like scaffolds positioned along the length of the perfusion core. The bioreactor system also uses a media gas exchange module that is free standing and does not require its inclusion in a CO2 incubator.
    Type: Grant
    Filed: March 5, 2015
    Date of Patent: February 26, 2019
    Assignee: Synthecon, Inc.
    Inventor: Stephen S. Navran, Jr.
  • Patent number: 9909097
    Abstract: This disclosure describes a biochemical reactor with an unclogging pipe. The biochemical reactor may include a tank configured to house immobilized carriers and fluid. The biochemical reactor may include a circulation conduit at least partially disposed within the tank. The circulation conduit may include a circulation inlet opening and a circulation outlet opening. The biochemical reactor may include one or more vanes disposed proximate to the circulation outlet opening. The one or more vanes may be configured to cause the immobilized carrier and the fluid exiting the circulation outlet opening to enter into a helical pattern. The biochemical reactor may include an unclogging pipe configured to clear clogging of the circulation conduit. The unclogging pipe may be disposed proximate to the circulation outlet opening. Clearing clogging of the circulation conduit may include directing a pressurized flow of air to the circulation conduit via the unclogging pipe.
    Type: Grant
    Filed: November 21, 2016
    Date of Patent: March 6, 2018
    Assignee: MIH WATER TREATMENT, INC.
    Inventor: Peter J. Hall
  • Patent number: 9909091
    Abstract: This disclosure describes a biochemical reactor with an unclogging pipe. The biochemical reactor may include a tank configured to house immobilized carriers and fluid. The biochemical reactor may include a circulation conduit at least partially disposed within the tank. The circulation conduit may include a circulation inlet opening and a circulation outlet opening. The biochemical reactor may include one or more vanes disposed proximate to the circulation outlet opening. The one or more vanes may be configured to cause the immobilized carrier and the fluid exiting the circulation outlet opening to enter into a helical pattern. The biochemical reactor may include an unclogging pipe configured to clear clogging of the circulation conduit. The unclogging pipe may be disposed proximate to the circulation outlet opening. Clearing clogging of the circulation conduit may include directing a pressurized flow of air to the circulation conduit via the unclogging pipe.
    Type: Grant
    Filed: March 23, 2017
    Date of Patent: March 6, 2018
    Assignee: MIH WATER TREATMENT, INC.
    Inventor: Peter J. Hall
  • Patent number: 9763977
    Abstract: Embodiments of the invention provide bio-reactor circuits for in vitro research applications. One embodiment provides a bio-reactor circuit comprising at least one bio-reactor and a pump fluidically coupled to the at-least-one bio-reactor. The bio-reactor comprises a housing having inlet and outlet ports and first and second chambers. The chambers are separated by a porous membrane with the first chamber providing a flow path for a fluid. The membrane includes a coating having a cell binding affinity for the attachment and proliferation of cells to cover the surface of the membrane. The second chamber provides a volume for maintaining the viability of cells disposed in the chamber. The cells can be selected to produce a biochemical compound. The membrane is configured to allow for diffusion of the compound from the second chamber into the flow path as well as allow for diffusion of gases, nutrients and other biochemical compounds.
    Type: Grant
    Filed: December 12, 2008
    Date of Patent: September 19, 2017
    Assignee: INCUBE LABS, LLC
    Inventor: Mir Imran
  • Patent number: 9677038
    Abstract: A compact, high-yield, bio-reactor for use as small, medium and large-scale production unit for hosting and culturing of stem cells or living cells or micro organism and producing by multiplication or expression one or more biologic compounds for medical and biological applications.
    Type: Grant
    Filed: June 21, 2011
    Date of Patent: June 13, 2017
    Assignee: STROBBE PHARMA TECH GMBH
    Inventor: Per Stobbe
  • Patent number: 9656264
    Abstract: Embodiments of the invention relate to a biological sample holder and for holding biological samples. Conventionally, processing of biological samples, which may be stored on a biological sample storage medium, is done manually with samples being tested individually. However, handling of the samples is difficult and time consuming; greater demand for storage and extraction of genetic material has led to a requirement for greater throughput. In embodiments of the present invention, there is provided a biological sample holder comprising a chamber holding a biological sample storage medium, the chamber comprising one or more openings for receiving a liquid when inserted therein. This provides a means of holding a biological sample which is easy to handle and suitable for automation, for example in an array of such holders, allowing processing of multiple biological samples in parallel.
    Type: Grant
    Filed: February 24, 2012
    Date of Patent: May 23, 2017
    Assignee: GE Healthcare UK Limited
    Inventor: Stevan Paul Tortorella
  • Patent number: 9575055
    Abstract: Disclosed herein are bioreactor systems and methods of utilizing said systems.
    Type: Grant
    Filed: June 18, 2010
    Date of Patent: February 21, 2017
    Assignee: KIYATEC INC.
    Inventors: Matthew R. Gevaert, David E. Orr
  • Patent number: 9534236
    Abstract: An example includes an apparatus, method, and system for producing and extracting a gas from a wastewater fluid. The apparatus can include a membrane including at least one hollow fiber, the at least one hollow fiber configured to convey a gas extracted from a wastewater fluid. Bacteria can be immobilized within the membrane, and the bacteria configured to produce the gas during treatment of the wastewater fluid.
    Type: Grant
    Filed: March 5, 2014
    Date of Patent: January 3, 2017
    Assignee: Regents of the University of Minnesota
    Inventors: Paige J. Novak, William A. Arnold, Erin M. Surdo
  • Patent number: 9317922
    Abstract: A system and method for recognition of images may include the use of alignment markers. The image recognized may be a pattern from an array, a character, a number, a shape, and/or irregular shapes. The pattern may be formed by elements in an array such as an identification marking and/or a sensor array. More particularly, the system and method relate to discriminating between images by accounting for the orientation of the image. The size and/or location of alignment markers may provide information about the orientation of an image. Information about the orientation of an image may reduce false recognitions. The system and method of image recognition may be used with identification markings, biosensors, micro-fluidic arrays, and/or optical character recognition systems.
    Type: Grant
    Filed: January 31, 2011
    Date of Patent: April 19, 2016
    Assignee: Board of Regents The University of Texas System
    Inventors: Jason E. Meiring, Timothy B. Michaelson, C. Grant Willson
  • Patent number: 9228579
    Abstract: A compact, high-yield, bio-reactor for use as small, medium and large-scale production unit for therapeutic proteins, proteins in general and enzymes based on controlled activity of the cells or micro organisms and the production of such as high value antibodies for pharmaceutical and/or bio-diagnostic applications.
    Type: Grant
    Filed: December 18, 2009
    Date of Patent: January 5, 2016
    Assignee: Stobbe Tech A/S
    Inventor: Per Stobbe
  • Patent number: 9226494
    Abstract: A modular bioreactor is provided that includes an upper biochamber; a lower biochamber; and an intermediate biochamber that is positioned between the upper biochamber and the lower biochamber. Each biochamber of the bioreactor is in fluid communication with each other biochamber of the bioreactor and includes an interior wall, which defines a centrally-disposed cavity for each biochamber, and an inflow port and an outflow port that are in fluid communication with each centrally disposed cavity. Systems and methods for vascularizing a tissue construct are also provided.
    Type: Grant
    Filed: September 18, 2009
    Date of Patent: January 5, 2016
    Assignee: University of Louisville Research Foundation, Inc.
    Inventors: Carlos C. Chang, James B. Hoying, Stuart K. Williams
  • Patent number: 9206384
    Abstract: A micro-incubator manifold for improved microfluidic configurations and systems and methods of manufacture and operation for a manifold and automated microfluidic systems.
    Type: Grant
    Filed: December 3, 2012
    Date of Patent: December 8, 2015
    Assignee: EMD Millipore Corporation
    Inventors: Philip J. Lee, Terry Gaige, Wei Hsuan (Jessie) Ho
  • Patent number: 9102910
    Abstract: The present invention relates to a bioreactor comprising a first fluid distribution chamber and a first fluid collection chamber, the reactor adapted to receive at least one conduit in fluid communication between the first fluid distribution chamber and the first fluid collection chamber; wherein the reactor includes a second fluid distribution means including a plurality of distributors arranged to distribute the second fluid between the first fluid distribution chamber and the first fluid collection chamber. The invention extends to a removable insert for a bioreactor comprising a first fluid distribution plate; a first fluid collection plate; and a second fluid distribution means including a plurality of distributors arranged to distribute the second fluid between the first fluid distribution plate and the first fluid collection plate.
    Type: Grant
    Filed: April 18, 2012
    Date of Patent: August 11, 2015
    Assignee: Synexa Life Sciences (Proprietary) Limited
    Inventors: Wade Edwards, P. G. Lourens
  • Publication number: 20150140624
    Abstract: A method and system for producing lactic acid from biomass materials uses a mixed bacteria culture of at least one homofermentative lactic acid bacteria and at least one heterofermentative lactic acid bacteria in an integrated production system to increase the productivity and yield of lactic acid.
    Type: Application
    Filed: June 15, 2012
    Publication date: May 21, 2015
    Applicant: Jiangsu University
    Inventor: Fengjie Cui
  • Patent number: 9034640
    Abstract: A system includes a first chamber configured to receive a hydrogel and a scaffold comprising a cell, wherein the hydrogel is in fluid communication with the scaffold, and wherein the hydrogel includes a plurality of unidirectional pores. The system also includes a second chamber configured to receive a first fluid and a second fluid, wherein the second chamber includes a wall that separates the first fluid from the second fluid. The system further includes a porous membrane configured to separate the first chamber from the second chamber. The wall is configured to move along the porous membrane as cellular extensions are projected into at least a portion of the plurality of unidirectional pores of the hydrogel.
    Type: Grant
    Filed: May 12, 2011
    Date of Patent: May 19, 2015
    Assignee: EMPIRE TECHNOLOGY DEVELOPMENT LLC
    Inventors: Marvi A. Matos, William B. Carlson, Ivan Vesely, Yansong Gu
  • Patent number: 9023642
    Abstract: A bioreactor and method that permits continuous and simultaneous short, moderate, or long term cell culturing of one or more cell types or tissue in a laminar flow configuration is disclosed, where the bioreactor supports at least two laminar flow zones, which are isolated by laminar flow without the need for physical barriers between the zones. The bioreactors of this invention are ideally suited for studying short, moderate and long term studies of cell cultures and the response of cell cultures to one or more stressors such as pharmaceuticals, hypoxia, pathogens, or any other stressor. The bioreactors of this invention are also ideally suited for short, moderate or long term cell culturing with periodic cell harvesting and/or medium processing for secreted cellular components.
    Type: Grant
    Filed: July 6, 2007
    Date of Patent: May 5, 2015
    Assignee: The University of Houston System
    Inventors: Stanley J. Kleis, Sandra K. Geffert, Steve R. Gonda
  • Publication number: 20150118737
    Abstract: There is provided a cell culture device including: a drug storing chamber; a cell culture chamber connected to the drug storing chamber and disposed in a position higher than that of the drug storing chamber; a pump unit circulating a drug from the drug storing chamber to the cell culture chamber; and an opening and closing unit closing an opened part of the drug storing chamber so that the drug storing chamber is isolated from ambient atmosphere.
    Type: Application
    Filed: January 3, 2014
    Publication date: April 30, 2015
    Applicant: SAMSUNG ELECTRO-MECHANICS CO., LTD.
    Inventors: Sang Jin KIM, Bo Sung KU
  • Publication number: 20150118745
    Abstract: A cell culture system having a cell culture vessel, a composition controlling fluid storage vessel, a culture fluid composition controlling means having an inlet and an outlet for a cell culture fluid, an inlet-connected fluid feeding circuit from the cell culture vessel to the inlet of the culture fluid composition controlling means, an outlet-connected fluid feeding circuit from the cell culture vessel to the outlet of the culture fluid composition controlling means, a means which perfuses the cell culture fluid from the inlet-connected fluid feeding circuit to the outlet-connected fluid feeding circuit through the culture fluid composition controlling means, and a means which controls the amount of fluid in the cell culture vessel, in which compositions of the cell culture fluid in the cell culture vessel and compositions of the composition controlling fluid in the composition controlling fluid storage vessel can be controlled in a continuous manner.
    Type: Application
    Filed: April 24, 2013
    Publication date: April 30, 2015
    Applicants: ASAHI KASEI KABUSHIKI KAISHA, TOKYO WOMEN'S MEDICAL UNIVERSITY
    Inventors: Ushio Iwamoto, Michi Sato, Kanako Konishi, Katsuhisa Matsuura, Tatsuya Shimizu, Teruo Okano
  • Patent number: 8969075
    Abstract: A method and apparatus for embedding cells that utilizes a flow-through embedding technique maximizes the efficiency of extractions and decreases time for embedding the cell fragments, minimizes cell loss, and automatically positions cell samples at the position in which a microtome blade will section them. The apparatus includes a cell flow pathway defined by an inflow tube for delivering cell fragments from a cell sample to a sample port. The sample port is in fluid communication with a tissue cassette having attached thereto a filter. The cell flow pathway is in communication with a reagent flow pathway for delivering the reagents through the sample port to the cassette. The apparatus is configured such that the application of pressure directs the cell fragments from the cell sample through the cell flow pathway, and effects delivery of the reagents through the reagent flow pathway.
    Type: Grant
    Filed: December 18, 2008
    Date of Patent: March 3, 2015
    Assignee: University of Massachusetts
    Inventor: Andrew H. Fischer
  • Publication number: 20140370588
    Abstract: In one aspect, an apparatus for bioprocessing using a liquid comprises an at least partially flexible vessel for receiving the liquid, and a filter adapted for moving within the liquid. An agitator may also be provided for agitating the liquid, and may be connected to the mixer. The mixer may spin or may not spin about an axis of rotation.
    Type: Application
    Filed: December 7, 2012
    Publication date: December 18, 2014
    Inventors: Jose Antonio Castillo Gonzalez, Vishwas Pethe
  • Patent number: 8906674
    Abstract: A sample preparation apparatus comprising: a storage chamber that can store therein a liquid sample including an analysis target to be analyzed; a concentrated sample storage chamber that is provided to communicate with the storage chamber and that stores therein concentrated liquid having an analysis target having a higher concentration than that of the liquid sample; and an analysis target transportation section for transporting the analysis target included in the liquid sample stored in the storage chamber to the concentrated sample storage chamber. A cell analyzer is also disclosed.
    Type: Grant
    Filed: September 29, 2010
    Date of Patent: December 9, 2014
    Assignee: Sysmex Corporation
    Inventors: Ryuichiro Ebi, Koki Tajima
  • Patent number: 8877489
    Abstract: Thin parylene C membranes having smooth front sides and ultrathin regions (e.g., 0.01 ?m to 5 ?m thick) interspersed with thicker regions are disclosed. The back sides of the membranes can be rough compared with the smooth front sides. The membranes can be used in vitro to grow monolayers of cells in a laboratory or in vivo as surgically implantable growth layers, such as to replace the Bruch's membrane in the eye. The thin regions of parylene are semipermeable to allow for proteins in serum to pass through, and the thick regions give mechanical support for handling by a surgeon. The smooth front side allows for monolayer cell growth, and the rough back side helps prevents cells from attaching there.
    Type: Grant
    Filed: January 20, 2012
    Date of Patent: November 4, 2014
    Assignees: California Institute of Technology, University of Southern California
    Inventors: Yu-Chong Tai, Bo Lu, Mark Humayun
  • Patent number: 8865460
    Abstract: Disclosed are multi-chambered cell co-culture systems. The systems can be utilized to encourage the growth and development of isolated cells in a dynamic three-dimensional in vitro environment. The cell chambers (10) of the system can be in biochemical communication with adjacent chambers containing cells of different types, but the different cell types are maintained physically separated from one another. In addition, the local environment of each cell chamber can be independently controlled. For example, fluid flow characteristics through a single cell chamber can be independently controlled and maintained for each separate chamber of the system.
    Type: Grant
    Filed: August 11, 2006
    Date of Patent: October 21, 2014
    Assignee: Clemson University Research Foundation
    Inventors: David E. Orr, Karen J. L. Burg
  • Publication number: 20140308688
    Abstract: A multiple flow-based microfluidic cell culture system that emulates mammalian physiology is provided. Tissue-mimicking cell cultures are connected by flow within a physiologically meaningful arrangement so that the pharmacokinetics of various agents to be tested in the system emulate in vivo conditions. The system includes at least two organ tissue modules, each organ tissue module including a first chamber containing an organ tissue cell, the first chamber including an inlet and an outlet for flow of an organ tissue cell-specific culture medium; a second chamber including an inlet and an outlet for flow of a blood material; and a semi-permeable membrane separating the first and second chambers. The flow of blood material through each organ tissue module is interconnected and the flow of tissue-cell specific culture medium is directed to a single organ tissue module.
    Type: Application
    Filed: December 7, 2012
    Publication date: October 16, 2014
    Inventors: Sonia Grego, Brian Rhys Stoner, Kristin Hedgepath Gilchrist, Timothy Raymond Fennell, Ann Pitruzzello
  • Patent number: 8852925
    Abstract: Apparatuses, systems, and methods are provided for growing and maintaining cells. A three-dimensional matrix, such as a hydrogel material, is seeded with cells and placed in a bioreactor having two compartments. The matrix is supported between the two compartments by first and second porous materials, which engage opposing surfaces of the matrix. A first media stream having certain properties is propagated through the first compartment, where it contacts one surface of the matrix via the first porous material. A second media stream having different properties is propagated through the second compartment such that it contacts the opposite surface of the matrix via the second porous material. Through migration of each stream at least partially into the matrix, various controlled gradients may be established within the matrix, encouraging growth of the cells. Such gradients include osmotic pressure, oscillating osmotic pressure, hydrostatic pressure, oxygen tension, and/or nutrient gradients.
    Type: Grant
    Filed: December 17, 2007
    Date of Patent: October 7, 2014
    Assignee: The Charlotte-Mecklenburg Hospital Authority
    Inventors: Craig Halberstadt, Richard Peindl
  • Publication number: 20140295531
    Abstract: The invention relates to the field of reduction of CO2 emission, more in particular to CO2 capture and conversion. The invention further relates to the culturing of algae. One object of the present invention is to provide an alternative method for capturing and conversion of CO2 from a gaseous stream.
    Type: Application
    Filed: August 10, 2012
    Publication date: October 2, 2014
    Applicant: Nederlandse Organisatie voor toegepast-natuurwetenschappelijk onderzoek TNO
    Inventors: Earl Lawrence Vincent Goetheer, Peter Geerdink, Ikenna Sunday Ngene, Leo Jacques Pierre Van Den Broeke
  • Patent number: 8846383
    Abstract: Disclosed are a method and a device for the biotechnological production of valuable products, in which a medium is fed to a bioreactor and is subjected to a fermentation process, the valuable product is gathered as a filtered permeate and/or concentrated retentate via a cross-flow filtration system that is mounted downstream thereof, and residues are once again fed to the bioreactor until being gathered as a retentate. Other materials can be fed to the bioreactor in a controlled manner in addition to the medium while the concentrated retentate and permeate can be gathered in a controlled manner. The fermentation process and the filtration process are regulated in a synchronized manner in an integrated system via a digital control unit.
    Type: Grant
    Filed: June 21, 2003
    Date of Patent: September 30, 2014
    Assignee: Sartorius AG
    Inventors: Reiner Luttmann, Wilfried Kappel, Toralf Gliem, Mohammad Saeed Ajam, Lars Boettcher, Bernd-Ulrich Wilhelm, Wolfgang Rietschel
  • Publication number: 20140273199
    Abstract: A system and method for converting waste and secondary materials into synthesis gas (syngas) through the use of a molten metal bath gasifier for the initial breakdown of waste feeds and an A/C plasma reactor for complete dissociation of waste feeds into syngas, and an anaerobic digester. The system includes a heat recovery and steam power generation process for the production of electricity. The system produces a net output of electricity above plant load sufficient for the co-production of renewable Hydrogen and Oxygen. The process does not require the use of fossil fuels or fossil feedstocks during normal operations, and it eliminates combustion produced stack emissions or landfill residuals.
    Type: Application
    Filed: March 17, 2014
    Publication date: September 18, 2014
    Inventors: Randall Cole, Seth Murphy, Ronald Bruer, Edmund Kaminski, Peter Sach
  • Patent number: 8828711
    Abstract: A flow chamber and method for detecting the presence of one more cell produced analytes under flow conditions. The flow chamber includes two compartments separated by a permeable membrane on which a plurality of cells may be positioned. The permeable membrane shields one or more analyte sensors positioned one compartment from the convective transport forces of a fluid flow within the other compartment to allow reliable and accurate detection of cell-produced analytes and determination of the concentration of cell-produced analytes.
    Type: Grant
    Filed: May 27, 2011
    Date of Patent: September 9, 2014
    Assignee: Drexel University
    Inventors: Dov Jaron, Kenneth A. Barbee, Allison M. Andrews
  • Patent number: 8822208
    Abstract: The bioreactor is for use in performing biological and/or biochemical reactions and includes a vessel, an agitator, a reaction assembly, and a harvesting outlet. The vessel of the bioreactor includes several ports including a mixing port, a reaction port, and a harvesting port. The agitator extends through the mixing port into the vessel while the harvesting outlet extends through the harvesting port and permits the withdrawal of reaction medium to another vessel. The reaction assembly extends through the reaction port into the vessel and has multiple components including a gas conduit adapted to introduce gas into a reaction medium in the vessel, a sampling device adapted to remove a portion of the reaction medium from the vessel without contamination of the remaining reaction medium, and an introduction conduit permitting the introduction of at least the reaction medium into the vessel.
    Type: Grant
    Filed: March 27, 2013
    Date of Patent: September 2, 2014
    Assignee: Becton, Dickinson and Company
    Inventor: Bunkim Chokshi
  • Publication number: 20140234955
    Abstract: There is provided a cell culture device including a cell chip receiving part coupled to a cell chip and having at least one passage through which a liquid-type medium is circulated, and a vortex generation part formed in the passage to generate a vortex in the liquid-type medium.
    Type: Application
    Filed: April 19, 2013
    Publication date: August 21, 2014
    Applicant: SAMSUNG ELECTRO-MECHANICS CO., LTD.
    Inventors: Bo Sung KU, Sang Jin KIM
  • Patent number: 8778629
    Abstract: A sterility test method includes: selecting strain and culture medium, preparing bacterial cultures, transcribing fingerprint characteristics in thermograms as indices to verify the characteristics, drawing the thermodynamic parameters of the thermogram, determining the positive judgment index and performing sterility test for the samples. A fully-enclosed bacteria collecting ampoule incubator includes bacteria collecting ampoule system, sample and liquid feeding system and peristalsis liquid discharge system. The sample and liquid feeding system is connected with the bacteria collecting ampoule system by the liquid intake tube; and the bacteria collecting ampoule system is connected with the peristalsis liquid discharge system by the liquid drainage tube. The invention is characterized by short inspection time, high sensitivity, high automation and accurate test results on microbial contamination. It can also provide the overall process curve on the growth conditions.
    Type: Grant
    Filed: June 28, 2011
    Date of Patent: July 15, 2014
    Assignee: 302 Military Hospital of China
    Inventors: Dan Yan, Xiaohe Xiao, Ping Zhang, Yongshen Ren, Cheng Jin
  • Patent number: 8778670
    Abstract: A method and apparatus for embedding cells that utilizes a flow-through embedding technique. The apparatus includes a cell flow pathway defined by an inflow tube for delivering cell fragments from a cell sample to a sample port. The sample port is in fluid communication with a tissue cassette having attached thereto a filter. The cell flow pathway is in communication with a reagent flow pathway for delivering the reagents through the sample port to the cassette. The apparatus is configured such that the application of pressure directs the cell fragments from the cell sample through the cell flow pathway, and effects delivery of the reagents through the reagent flow pathway. The apparatus produces an embedded cell block having concentrated cells near the plane of the block to be sectioned in a quick and efficient manner.
    Type: Grant
    Filed: September 22, 2011
    Date of Patent: July 15, 2014
    Assignee: University of Massachusetts
    Inventor: Andrew H. Fischer
  • Patent number: 8765456
    Abstract: The invention relates to microscale cell separating apparatus which are able to separate cells on the basis of size of the cells, interaction of the cells with surfaces of the apparatus, or both. The apparatus comprises a stepped or sloped separation element (16) interposed between an inlet region (20) and an outlet region (22) of a void that can be tilled with fluid. The void can be enclosed within a cover (12) and fluid flow through the void engages cells with the separation element. Only cells which have (or can deform to have) a characteristic dimension smaller than or equal to the distance between a step and the cover or body can pass onto or past a step. Modifications of surfaces within the apparatus can also inhibit passage of cells onto or past a step.
    Type: Grant
    Filed: June 28, 2011
    Date of Patent: July 1, 2014
    Assignee: Parsortix, Inc.
    Inventor: Georgi Hvichia
  • Publication number: 20140170744
    Abstract: There is provided a cell culture device including a cell chip accommodation unit accommodating a cell chip therein, a drug storage unit storing a drug and having a closed structure, a first pipe connecting the drug storage unit and the cell chip accommodation unit and transferring the drug from the drug storage unit to the cell chip accommodation unit, a second pipe connecting the drug storage unit and the cell chip accommodation unit and transferring the drug from the cell chip accommodation unit to the drug storage unit, and a pump circulating the drug between the cell chip accommodation unit and the drug storage unit.
    Type: Application
    Filed: March 4, 2013
    Publication date: June 19, 2014
    Applicant: SAMSUNG ELECTRO-MECHANICS CO., LTD.
    Inventors: Sang Jin KIM, Bo Sung KU
  • Publication number: 20140154795
    Abstract: A perfusion bioreactor includes at least one ultrasonic transducer that can acoustically generate a multi-dimensional standing wave. The standing wave can be used to retain cells in the bioreactor, and can also be utilized to dewater or further harvest product from the waste materials produced in a bioreactor.
    Type: Application
    Filed: February 7, 2014
    Publication date: June 5, 2014
    Inventors: Bart Lipkens, Louis Masi, Stanley Kowalski, III, Walter M. Presz, JR., Jason Dionne, Brian Dutra, Ari Mercado, Thomas J. Kennedy, III, Arthur Martin
  • Patent number: 8715393
    Abstract: Disclosed is a method for removing carbon dioxide from a gas stream, comprising placing the gas stream in contact with a resin, wetting the resin with water, collecting water vapor and carbon dioxide from the resin, and separating the carbon dioxide from the water vapor. The resin may be placed in a chamber or a plurality of chambers connected in series wherein the first chamber contains resin that was first contacted by the gas, and each successive chamber contains resin which has been wetted and carbon dioxide collected from for a greater period of time than the previous chamber, and so on, until the last chamber. Secondary sorbents may be employed to further separate the carbon dioxide from the water vapor.
    Type: Grant
    Filed: April 17, 2008
    Date of Patent: May 6, 2014
    Assignee: Kilimanjaro Energy, Inc.
    Inventors: Allen B. Wright, Klaus S. Lackner, Ed Leon-Guerrero, Ursula Ginster, Tymothy Catterson, Karl Madison, Ryuhei Ishikawa, George W. Grimm, Mark Malcomson, Ping Liu
  • Publication number: 20140120610
    Abstract: In order to enable the inside of a constant-temperature device to be sterilized using sterilization gas and prevent contamination during incubation, even when provided with an atmosphere measuring means having a CO2 sensor and an oxygen concentration sensor, a sensor unit (100) is arranged inside or close to an incubation chamber, and the internal atmosphere is measured by sucking-in the atmosphere of the incubation chamber (2) by means of an air-flow generation means (20). Furthermore, high-precision filters (23, 24) are arranged in the flow channel through which the atmosphere within the incubation chamber (2) is taken-in/discharged, preventing bacteria and/or cells from flowing in, and also preventing the diffusion of sterilization gas to the inside of a sensor unit (100) during sterilization.
    Type: Application
    Filed: June 11, 2012
    Publication date: May 1, 2014
    Inventors: Seishi Yamashita, Tomonori Miwada
  • Publication number: 20140093952
    Abstract: A bioreactor hollow fiber perfusion system increases the capacity of standard fed batch bioreactors. The bioreactor hollow fiber perfusion system cycles bioreactor mass through a hollow fiber tangential flow filter which separates the metabolic wastes (as well as proteins) from the biomass material allowing the reactions in the bioreactor to continue when compared to a fed batch bioreactor. The bioreactor hollow fiber perfusion system preferably includes a low shear gamma stable disposable pumphead responsible for biomass re-cycling and can be easy installed or replaced without the risk of contamination.
    Type: Application
    Filed: October 2, 2012
    Publication date: April 3, 2014
    Inventor: David Serway