Abstract: The invention relates to a single-use connection device for insertion into a connection opening of a bioreactor, in particular in a connection opening of a top plate of a dimensionally-stable bioreactor or in a connection opening of a connection element of a pouch bioreactor. The single-use connection device is inserted in a connection opening of a top plate of a bioreactor and/or in a connection opening of a pouch reactor and is provided with multiple passages and an attachment section, wherein the single-use connection device is formed as a single piece and is made of plastic. The attachment section, on an outer peripheral surface, has an attachment structure for attaching the single-use connection device in the connection opening.

Abstract: A container system includes a flexible bag, a probe port, and a probe. The probe port includes an elongated tubular member having an interior surface bounding a first passageway extending between a first end and an opposing second end, the tubular member being made of a polymeric or elastomeric material and being flexible. The probe port also includes a flange coupled to the tubular member and radially outwardly projecting therefrom, the flange being secured to the flexible bag so that the first end of the tubular member projects into a chamber of the flexible bag while the second end of the first passageway of the tubular member is accessible from outside of the flexible bag. The probe can be received within the first passageway of the probe port.

Abstract: Disclosed herein are systems, methods, and devices for monitoring and controlling bioprocess parameters. The systems and methods enable automated operation with real-time analysis of process conditions and analyte or biologic production.

Abstract: Systems, devices, and methods of the present disclosure assist with management of tubes and hoses during surgical procedures. The systems, devices, and methods provide for the proper opening and closing of tubes to facilitate performance of steps in a surgical procedure. Systems, devices, and methods of the present disclosure control fluid delivery to and from a medical device, including devices for tissue processing and cleaning.

Abstract: Disclosed is a rotating culture vessel based on a rotating culture technology using an RWV, by which cell seeding, liquid medium exchange, quality control and so on can be automated and degassing can be conducted simultaneously with liquid medium exchange without disturbing the cells under culture. Also disclosed is an automatic cell culture apparatus using the same. A rotating culture vessel, which contains cells and a liquid culture medium, to be attached to a horizontal rotating shaft of a rotating culture device to three-dimensionally culture the cells, wherein one or more inlets/outlets for supplying cells and a liquid culture medium at the early stage and then taking out the cultured cells, are formed at appropriate position of a flat cylindrical culture container; at least one pair of a supply port and a discharge port for liquid medium exchange is provided on the outer circumferential cylindrical face of the culture container.

Abstract: In a cell culture in a sealed cell culture container, gradual deterioration of culture environment is prevented.

Abstract: The invention is directed to a centrifuge chamber comprising a cylinder having a base plate and a cover plate, a rotational axis with at least one port for input and/or output of liquids, at least one port for input of gases and at least one layer for cell culturing, wherein the layer for cell culturing comprises a gas-permeable membrane on which cells can be cultured and wherein the at least one port for input and/or output of gases is connected to the gas-permeable membrane.

Abstract: Disclosed are a method and a device for the biotechnological production of valuable products, in which a medium is fed to a bioreactor and is subjected to a fermentation process, the valuable product is gathered as a filtered permeate and/or concentrated retentate via a cross-flow filtration system that is mounted downstream thereof, and residues are once again fed to the bioreactor until being gathered as a retentate. Other materials can be fed to the bioreactor in a controlled manner in addition to the medium while the concentrated retentate and permeate can be gathered in a controlled manner. The fermentation process and the filtration process are regulated in a synchronized manner in an integrated system via a digital control unit.

Abstract: The bioreactor is for use in performing biological and/or biochemical reactions and includes a vessel, an agitator, a reaction assembly, and a harvesting outlet. The vessel of the bioreactor includes several ports including a mixing port, a reaction port, and a harvesting port. The agitator extends through the mixing port into the vessel while the harvesting outlet extends through the harvesting port and permits the withdrawal of reaction medium to another vessel. The reaction assembly extends through the reaction port into the vessel and has multiple components including a gas conduit adapted to introduce gas into a reaction medium in the vessel, a sampling device adapted to remove a portion of the reaction medium from the vessel without contamination of the remaining reaction medium, and an introduction conduit permitting the introduction of at least the reaction medium into the vessel.

Abstract: There is provided a bioreactor which is provided with a lid (13) that facilitates access to the incubation cavity. Specifically the end wall of the incubation cavity is constituted by the lid (13) so that removal of the cap renders the incubation cavity fully accessible.

Abstract: A stable system for producing liquid products such as ethanol, butanol and other chemicals from syngas components contacts CO or a mixture of CO2 and H2 with a hydrophilic membrane under anaerobic conditions and transfers these components into contact with microorganisms contained as a biofilm on the membrane. Maintaining the microorganisms as a biolayer on the surface of the membrane facilitates cleaning of the membrane surface that retains the biofilm. In addition the shell gas space that surrounds the membranes may be flooded to reduce or remove the biofilm. Agitation of the liquid, by for example the bubbling of gas in the surrounding shell space, can fully or partially remove the biofilm from the membrane.

Abstract: A method of manufacturing biological products in a single container from the growth of cells to purification of the product is performed in a flexible disposable bioreactor that uses only a compressed gas for mixing and gasification. A porous septum is used to create the gasification and mixing as well as to separate a chromatography media used to harvest and purify a biological product in the same container. The closed container can be used in any environment without the risk of contamination to the product or the risk of contamination of environment with the product. This allows large manufacturing of hazardous substances, drugs and vaccines anywhere at the lowest cost possible. Numerous applications can be found in counter-terrorism and biodefense operations as well in managing epidemic illnesses.

Abstract: The present invention describes a system and method for accurately measuring the concentration of a substance within a filter housing. A concentration sensor and a communications device are coupled so as to be able to measure and transmit the concentration of a particular substance within the filter housing while in use. This system can comprise a single component, integrating both the communication device and the concentration sensor. Alternatively, the system can comprise separate sensor and transmitter components, in communication with one another. In yet another embodiment, a storage element can be added to the system, thereby allowing the device to store a set of concentration values. The use of this device is beneficial to many applications. For example, the ability to read concentration values in situ allows integrity tests to be performed without additional equipment.

Abstract: A modular membrane supported bioreactor for anaerobic conversion of gas into liquid products including membrane module(s) having a plurality of hollow fibers packed across a cross sectional area of the membrane module in a direction transverse to the axis of the membrane module; a membrane vessel for retaining the membrane module(s) at least partially submerged in a process liquid and isolated from ambient atmosphere; and a gas supply conduit operably connected to the hollow fibers for supplying a process gas to the hollow fiber lumens. The gas supply conduit enables the formation of a biofilm on the outer surface of the hollow fiber wall by interaction of microorganisms with the process gas and the production of a liquid product that mixes with the process liquid.

Abstract: A method and system for culturing cells, having a substantially airtight enclosure configured to culture cells. The method and system also have a first conduit configured to provide a reduced pressure to the substantially airtight enclosure and a second conduit configured to provide a culture media to the substantially airtight enclosure.

Abstract: A stable system for producing liquid products such as ethanol, butanol and other chemicals from syngas components contacts CO or a mixture of CO2 and H2 with a hydrophilic membrane under anaerobic conditions and transfers these components into contact with microorganisms contained as a biofilm on the membrane. Maintaining the microorganisms as a biolayer on the surface of the membrane facilitates cleaning of the membrane surface that retains the biofilm. In addition the shell gas space that surrounds the membranes may be flooded to reduce or remove the biofilm. Agitation of the liquid, by for example the bubbling of gas in the surrounding shell space, can fully or partially remove the biofilm from the membrane.

Abstract: A device for applying an axial compressive force to a fluid contamination detection system comprising a cup joined by a frangible connection to a filter ring, the force being sufficient to break the frangible connection and collapse a portion of the ring into the cup without damaging the ring. The device includes a stop element and a platform and an actuating mechanism for reciprocally moving the platform relative to the stop element. In a first position, the distance between the platform and the stop element is greater than the axial length of the detection system when the cup and the filter ring of the funnel are connected to each other, and in a second position, the distance between the platform and the stop element is less than the axial length of the detection system when the cup and the filter ring of the funnel are connected to each other.

Abstract: The culture chamber of the present invention has a fluid-filled culture compartment in which cells, tissues and other biologicals are cultured. The culture compartment is transversed by one or more molecular weight cut-off membranes attached to a membrane carrier assembly. Incoming nutrients are transported through the membrane into the culture compartment and metabolic waste products are transported away from the fluid-filled culture compartment through the membrane and out the chamber outlet. Both reusable and disposable culture chambers are described for culturing cells, cell aggregates, particles, tissues and organoids.

Abstract: The present invention relates to a bioreactor for sewage and wastewater treatment, which is capable of agitating and supplying oxygen simultaneously. A bioreactor of the present invention, comprises a culture drum in which microorganisms are cultured; a supplier for supplying a culture solution, and organic and inorganic media to the culture drum, the supplier including a supply tube connected to an upper side of the culture drum, and a pump connected to the supply tube for supplying the culture solution to the culture drum; an agitator for agitating the culture solution by spraying air into the culture solution in the culture drum, the air being supplied from an air blower; and a thermostatic controller for controlling a temperature of the culture solution and disinfecting the interior of the culture drum.

Abstract: The bioartificial reactor has a reactor vessel. A cell culture chamber in the reactor, in particular for liver cells, is divided off from a flow chamber for a nutritive medium and from a flow chamber for plasma and blood by semipermeable walls. At least one flow chamber is formed as a tube that is helically wound in the shape of a screw and that is made of semipermeable material.

Abstract: Apparatus and methods are directed to a perfusion culture system in which a rotating bioreactor is used to grow cells in a liquid culture medium, while these cells are attached to an adhesive-treated porous surface. As a result of this arrangement and its rotation, the attached cells divide, with one cell remaining attached to the substrate, while the other cell, a newborn cell is released. These newborn cells are of approximately the same age, that are collected upon leaving the bioreactor. The populations of newborn cells collected are of synchronous and are minimally, if at all, disturbed metabolically.

Abstract: A substantially continuous filtration or separation system and related processes are disclosed. More specifically, a continuous perfusion cell culture system employing an external spin filtration system for the production of proteins is disclosed.

Abstract: A cell culture bag for culturing cells, cellular aggregates, particles, tissues and organoids is disclosed. The cell culture bag is a flexible and disposable culture bag that fits within a bag support chamber of a bioreactor. The cell culture bag has an inlet means and an outlet means for the introduction and removal of media. Certain embodiments of the culture bag have an inlet and outlet fluid coupling swivel joint and/or perforated perfusion tubes extending from the inlet means into the interior of the bag to assist in mass transfer and mixing the media to maintain an even temperature throughout the culture bag.

Abstract: The invention concerns the use of filtering means comprising a housing (2) wherein are arranged parallel to one another a plurality of hollow fibres (3), whereof the porous wall (3a) of each defines an aperture (4), so that said means comprises a inner path and an outer path (B) corresponding to the volume available between the fibres and the disc separator for treating an initial sample with large volume comprising in diluted state a plurality of micro-organisms of different sizes. The invention is characterised in that during at least a concentration step, one single means of filtering is available, the atomic mass unit threshold of the porous wall (3a) of the fibres is determined so as to retain the smallest micro-organism of the initial sample, said means being used in frontal mode and discontinuously, thereby providing the output sample liquid, that is the concentrate.