Testing For Antimicrobial Activity Of A Material Patents (Class 435/32)
  • Patent number: 10995357
    Abstract: Single dye fluorescent staining and the combination of differences in both intensity and spectral emission permit determination of the minimum concentration of an antibiotic needed to inactivate bacteria (Minimum Inhibitory Concentration (MIC)), thereby providing a means for rapid Antibiotic Susceptibility Testing (AST). This allows for a quick and easy means for clinicians to determine a suitable treatment regimen for patients suffering from bacterial infections and those that eventually lead to sepsis.
    Type: Grant
    Filed: January 9, 2018
    Date of Patent: May 4, 2021
    Inventors: Gal Ingber, Moshe Ben-David, Mushki Fridman, Yael Gluckman, Dina Gohman, Orly Hammer Munz, Anita Shinderman, Eran Zahavy
  • Patent number: 10907158
    Abstract: Provided are antisense morpholino oligomers targeted against bacterial virulence factors such as genes that contribute to antibiotic resistance or biofilm formation, or essential genes, and related compositions and methods of using the oligomers and compositions, for instance, in the treatment of an infected mammalian subject.
    Type: Grant
    Filed: December 22, 2016
    Date of Patent: February 2, 2021
    Assignees: Board of Regents, The University of Texas System, Oregon State University
    Inventors: David Greenberg, Bruce L. Geller, Erdal Toprak
  • Patent number: 10870876
    Abstract: A method for detecting a presence or an absence of at least one zone of inhibition, the method including a step consisting in depositing a volume of the sample in liquid form along a deposition zone extending along an axis at the surface of the agar culture medium and a step consisting in depositing a determined amount of a chemical agent at the surface of the agar culture medium, the deposit defining a potential zone of inhibition, the axis of the zone of deposition of the sample intersecting the potential zone of inhibition.
    Type: Grant
    Filed: July 6, 2016
    Date of Patent: December 22, 2020
    Assignee: BIOMERIEUX
    Inventors: Laurent Drazek, Agnés Dupont-Fillard, Frédéric Pinston, Hervé Rostaing
  • Patent number: 10829797
    Abstract: A method for determining the antimicrobial susceptibility of a microorganism in a clinical sample includes removing a test aliquot from a clinical sample culture before the culture reaches 0.5 McFarland units and isolating the microbial cells. The cells are transferred into a suitable culture medium for microbial growth and an AST assay is performed using the isolated microbes. The concentration of microbial cells in the microbial cells used to set up the AST assay is measured before measuring the degree of microbial growth in different conditions of the AST assay. Devices for determining the anti-microbial susceptibility of a microorganism in a clinical sample are also disclosed.
    Type: Grant
    Filed: June 16, 2016
    Date of Patent: November 10, 2020
    Assignee: Q-linea AB
    Inventors: Jonas Jarvius, Jan Grawe, Ylva Molin, Markus Klintstedt, Mats Gullberg
  • Patent number: 10752959
    Abstract: Aspects of the present invention provide novel multi-targeted microbiological screening and monitoring methods having substantial utility for monitoring and control of microbial growth and contaminants, microbiological processes, predictive microbiology, and for exposure and risk assessment. Microbial markers shared by both target and index microbes are used in novel methods for microbial monitoring, monitoring of microbial performance potential, trend analysis, and statistical process control (SPC) in processes or systems that are receptive to a plurality of genetically distinct microbes.
    Type: Grant
    Filed: January 15, 2015
    Date of Patent: August 25, 2020
    Assignee: Institute for Environmental Health, Inc.
    Inventor: Mansour Samadpour
  • Patent number: 10711248
    Abstract: A method and device for standardizing myoblast differentiation into myotubes, including a substrate (1) and at least one cell-adhesive pattern (2) for culturing myoblasts on the substrate. The pattern (2) has an elongated surface. A central region (2C) and two lateral regions (2L) extend from the central region in both directions along a longitudinal axis of the pattern. The ratio between the maximum width (WC) of the central region (2C) and the maximum width (WL) of the lateral regions (2L) is greater than or equal to 2. The ratio between the length (L) and the maximum width (WC) of the pattern (2) is less than or equal to 4. The method includes providing a device as described above, depositing myoblasts on at least one cell-adhesive pattern of the device, culturing the myoblasts in a differentiation medium to promote cell differentiation into myotubes and constrain elongation of the myotubes.
    Type: Grant
    Filed: December 17, 2014
    Date of Patent: July 14, 2020
    Assignee: CYTOO
    Inventors: Mathieu Fernandes, Sebastien Degot, Yoran Margaron, Jie Liu, Michel Bornens, Maria Luisa Calvo Munoz, Aurelie Berthelot, Alexandra Fuchs, Joanne Young, Delphine Morales
  • Patent number: 10653676
    Abstract: Provided are small molecule inhibitors of ubiquitin specific protease 1 (USP1) activity and methods for their use in treating and characterizing cancers. The small molecule USP1 inhibitors of the invention are particularly useful in the treatment of cancers that are resistant to DNA cross-linking agents.
    Type: Grant
    Filed: November 3, 2016
    Date of Patent: May 19, 2020
    Assignees: Dana-Farber Cancer Institute, Inc., The Brigham and Women's Hospital, Inc.
    Inventors: Alan D. D'Andrea, Gregory D. Cuny, Ross L. Stein, Marcie Glicksman, April Case, Jun Xian, David Wilson, Min Huang
  • Patent number: 10549272
    Abstract: A container for storing a biological sample for molecular diagnostic testing and/or histological testing is provided. The container includes a first chamber for receiving a sample holder therein, a second chamber, and a closure for enclosing the container. A breakable membrane, such as a piercable foil, extends within the container and separates the two chambers. When the breakable membrane is broken, fluid can pass between the first and second chambers. The membrane may be broken through an activator on the closure, such as a depressible member or a rotatable carrier, causing the sample holder to break through the membrane.
    Type: Grant
    Filed: May 23, 2017
    Date of Patent: February 4, 2020
    Assignee: Becton, Dickinson and Company
    Inventors: Benjamin R. Bartfeld, C. Mark Newby
  • Patent number: 10499655
    Abstract: The present disclosure provides an inhibitor for inhibiting or disrupting biofilm and use thereof. Specifically, the present disclosure an application of a PsIG protein and a coding sequence thereof in inhibiting or disrupting a biofilm of a microorganism. The present disclosure also provides a composition and a preparation containing the PsIG protein to inhibit or disrupt a biofilm of a microorganism. The experiment shows that PsIG can effectively inhibit or disrupt a biofilm of Pseudomonas species, suggesting the great potential of PsIG in clinical and environmental fields.
    Type: Grant
    Filed: March 11, 2016
    Date of Patent: December 10, 2019
    Assignee: INSTITUTE OF MICROBIOLOGY, CHINESE ACADEMY OF SCIENCES
    Inventors: Lvyan Ma, Shan Yu, Huijun Wu, Shiwei Wang, Di Wang
  • Patent number: 10487362
    Abstract: Provided are compositions and methods for detecting in a sample the presence or absence, and/or the amount, of a small nucleolar RNA (snoRNA) HBII-52, also known as SNORD115. The compositions and methods are useful in diagnosis, prognosis, therapy recommendations, therapy, and monitoring of therapy for individuals who have a disorder that is positively correlated with elevated HBII-52, such as cancer, and particularly for prostate cancer. Kits containing primers for detecting and/or amplifying HBII-52 from a biological sample are provided. The disclosure includes a method for monitoring an individual undergoing therapy for a disorder associated with HBII-52 expression, a method for identifying an individual as a candidate for therapy with an antagonist of 5-HT2cR, and a method for therapy by administering to a subject a therapeutically effective amount of an antagonist of 5-HT2cR.
    Type: Grant
    Filed: January 9, 2014
    Date of Patent: November 26, 2019
    Assignee: Health Research, Inc.
    Inventors: Barbara Foster, Steven J. Seedhouse, Jason Kirk
  • Patent number: 10465227
    Abstract: The present disclosure provides for microbial testing devices, methods for making microbial testing devices, methods for growing and testing microbial cultures and methods for testing compounds for antimicrobial activity.
    Type: Grant
    Filed: January 23, 2015
    Date of Patent: November 5, 2019
    Assignee: BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEM
    Inventors: Anand K. Ramasubramanian, Jose L. Lopez-Ribot, Anand Srinivasan
  • Patent number: 10457972
    Abstract: The invention relates to a mass spectrometric method for determining microbial resistances to antibiotics. The invention provides specific methods comprising cultivation in synthetic media, in which several amino acids, preferably only a single amino acid, are isotopically labeled by incorporating 13C, 15N, 18O or 34S. If several amino acids are isotopically labeled, they are labeled in such a way that they are each heavier than the corresponding unlabeled amino acids by the same integer mass difference ?m. This ensures that the mass shifts of the peaks always amount to an integer multiple of the mass difference ?m. The total mass difference can be kept relatively small by selecting suitable amino acids. A mass shift of the protein peaks in media with antibiotics indicates that the microbes are resistant. A second embodiment first produces isotopically labeled microbes, which are then tested for their resistance by cultivating them in normal media.
    Type: Grant
    Filed: May 7, 2014
    Date of Patent: October 29, 2019
    Inventors: Jochen Franzen, Markus Kostrzewa, Christoph Lange, Katrin Sparbier
  • Patent number: 10414648
    Abstract: Method for making a cavity, comprising steps to: make a hole in a cover covering a sacrificial portion on a substrate; make a first layer on the cover, either in a first configuration covering a first region of the cover around the hole, or in a second configuration surrounding a first region of the cover around the hole; deposit a second layer covering the cover and the first layer and with, in a first configuration a low adhesion force between the first and second layers, or in a second configuration a low adhesion force between the second layer and the first region; etch the second layer, forming a flap of which a first part does not hermetically close or seal the hole, and of which a second end is fixed to a second region of the cover; etch the sacrificial portion; close the cavity.
    Type: Grant
    Filed: December 20, 2016
    Date of Patent: September 17, 2019
    Assignee: COMMISSARIAT A L'ENERGIE ATOMIQUE ET AUX ENERGIES ALTERNATIVES
    Inventors: Bruno Reig, Damien Saint-Patrice
  • Patent number: 10336977
    Abstract: A bioreactor for cell co-culture having at least first and second cell culture chambers which are separated by a porous membrane. The membrane has at least one sealing gasket integrated in the membrane and being integral therewith. The sealing gasket defines a closed perimeter delimiting a first cell culture area.
    Type: Grant
    Filed: March 5, 2015
    Date of Patent: July 2, 2019
    Assignees: INSTIT D'INVESTIGACIONS BIOMEDIQUES AUGUST PI I SUNYER (IDIBAPS), CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS, CIBER CENTRO DE INVESTIGATIÓN BIOMÉDICA EN RED, HOSPITAL CLINIC DE BARCELONA, UNIVERSITAT DE BARCELONA
    Inventors: Carmen Peralta Uroz, Jordi Gracia Sancho, Rosa Villa Sanz, Javier Illa Vila, Marta Massip Salcedo, Jaime Bosch Genover
  • Patent number: 10246698
    Abstract: An intermediate composite capable of transferring a biological or chemical material to be patterned on a surface. The intermediate composite includes a hydrogel, and particles suspended in the hydrogel, generating a particle-gel composite (composite), the composite is configured to absorb a biological or chemical material (agent), and further configured to deposit the agent when the composite is positioned proximate to a surface on which the agent is to be deposited.
    Type: Grant
    Filed: December 25, 2017
    Date of Patent: April 2, 2019
    Assignee: Purdue Research Foundation
    Inventors: Cagri A. Savran, Bin-Da Chan, Richard Lee Gieseck, III
  • Patent number: 10139406
    Abstract: A method of determining the P. aeruginosa or related species infection status of a subject comprising: (i) providing a sample of material obtained from the subject; (ii) determining the level of one or more alkyl quinolones or metabolites thereof produced by P.
    Type: Grant
    Filed: May 13, 2014
    Date of Patent: November 27, 2018
    Assignee: The University of Nottingham
    Inventors: Miguel Camara, Paul Williams, David Barrett, Nigel Halliday, Alan Knox, Alan Smyth, Andrew Fogarty, Helen Barr, Doug Forrester
  • Patent number: 9926526
    Abstract: Provided herein are methods and systems for interfering with viability of bacteria and related compounds and compositions.
    Type: Grant
    Filed: April 25, 2012
    Date of Patent: March 27, 2018
    Assignee: CALIFORNIA INSTITUTE OF TECHNOLOGY
    Inventors: Dianne K. Newman, Ryan C. Hunter
  • Patent number: 9902989
    Abstract: Presented herein are methods and compositions for the detection of specific beta-lactamases, including class A serine carbapenemases, metallo-beta-lactamases, AmpC beta-lactamases, and extended-spectrum beta-lactamases (ESBLs). The methods presented herein include methods that permit the detection of the presence of specific beta-lactamases in bacterial samples within as few as 2 to 10 minutes.
    Type: Grant
    Filed: June 22, 2015
    Date of Patent: February 27, 2018
    Assignee: Becton, Dickinson and Company
    Inventors: Mei Yang-Woytowitz, Charles Yu, Timothy Wiles
  • Patent number: 9850478
    Abstract: An intermediate composite capable of transferring a biological or chemical material to be patterned on a surface. The intermediate composite includes a hydrogel, and particles suspended in the hydrogel, generating a particle-gel composite (composite), the composite is configured to absorb a biological or chemical material (agent), and further configured to deposit the agent when the composite is positioned proximate to a surface on which the agent is to be deposited.
    Type: Grant
    Filed: August 12, 2015
    Date of Patent: December 26, 2017
    Assignee: Purdue Research Foundation
    Inventors: Cagri A. Savran, Bin-Da Chan, Richard Lee Gieseck, III
  • Patent number: 9732370
    Abstract: The present invention provides a method and test for the determination of the presence or absence of an antibiotic in a sample such as milk.
    Type: Grant
    Filed: April 30, 2014
    Date of Patent: August 15, 2017
    Assignee: DSM IP ASSETS B.V.
    Inventor: Dhiredj Chandre Jagesar
  • Patent number: 9709469
    Abstract: Provided are methods, devices and systems for analyte detection using a microfluidic device.
    Type: Grant
    Filed: November 11, 2012
    Date of Patent: July 18, 2017
    Assignee: The Regents of the University of California
    Inventors: Wenwan Zhong, Runtao Zhong
  • Patent number: 9689021
    Abstract: The present invention relates to methods for determining the concentration of a free beta-lactam antibiotic in a biological sample. In particular, the present invention relates to methods for measuring a free beta-lactam antibiotic in a biological sample, comprising the steps of: (a) providing at least one class C beta-lactamase, a functional fragment or variant thereof; (b) providing at least one biological sample; (c) contacting said at least one class C beta-lactamase with said at least one biological sample; and (d) determining the concentration of said free beta-lactam antibiotic in said at least one biological sample.
    Type: Grant
    Filed: October 15, 2012
    Date of Patent: June 27, 2017
    Assignees: UNIVERSITÉ DE LIÈGE, UNIVERSITÉ CATHOLIQUE DE LOUVAIN, UNIVERSITÉ LIBRE DE BRUXELLES, UNIVERSITÉ DE MONS-HAINAUT
    Inventors: Alain Brans, Bernard Joris, Michael Delmarcelle, Jacqueline Marchand, Paul Tulkens, Catherine Hammaecher, Erik Goormaghtigh, Joel De Coninck
  • Patent number: 9611501
    Abstract: Described herein is a method and a device for expediting delivery of an agent to a damaged bacterial cell. In one embodiment, the methods and devices are useful for screening candidate antibiotics. In another embodiment, the methods and devices described herein are used to determine susceptibility of bacteria to an antibiotic. The methods also provide a method for determining an appropriate antibiotic to treat an individual having a bacterial infection.
    Type: Grant
    Filed: May 29, 2014
    Date of Patent: April 4, 2017
    Assignees: Trustees of Boston University, Fraunhofer USA, INC.
    Inventors: Alexis Fay Sauer-Budge, Andre Sharon, Maxim Kalashnikov, Holger Wirz
  • Patent number: 9523112
    Abstract: The present invention provides a method and test for the determination of the presence or absence of an antibiotic in a sample such as milk.
    Type: Grant
    Filed: October 18, 2012
    Date of Patent: December 20, 2016
    Assignee: DSM IP ASSETS B.V.
    Inventors: Dhiredj Chandre Jagesar, Tim De Graaf, Truke Widel Smoor
  • Patent number: 9492538
    Abstract: A small percentage of cells within an established tumor have the properties of stem cells. These solid tumor stem cells give rise both to more tumor stem cells and to the majority of cells in the tumor that have lost the capacity for extensive proliferation and the ability to give rise to new tumors. The solid tumor heterogeneity reflects the presence of tumor cell progeny arising from a solid tumor stem cell. This discovery is the basis for solid tumor stem cell compositions, methods for distinguishing functionally different populations of tumor cells, methods for using these tumor cell populations for studying the effects of therapeutic agents on tumor growth, and methods for identifying and testing novel anti-cancer therapies directed to solid tumor stem cells.
    Type: Grant
    Filed: June 10, 2005
    Date of Patent: November 15, 2016
    Assignee: The Regents of the University of Michigan
    Inventors: Michael F. Clarke, Sean J. Morrison, Max S. Wicha, Muhammad Al-Hajj
  • Patent number: 9448170
    Abstract: A biological growth inhibition factor assay method includes: a first step of mixing a photosynthetic sample, with an aqueous solution sample to prepare a test measurement solution, letting the test measurement solution stand, and then after illuminating light onto the test measurement solution for a predetermined illumination time, measuring the light amount of the delayed fluorescence that is emitted; a second step of mixing the photosynthetic sample with a standard sample, in which biological growth inhibition factors are not present, to prepare a standard measurement solution, letting the standard measurement solution stand, and then after illuminating light onto the standard measurement solution for a predetermined illumination time, measuring the light amount of the delayed fluorescence that is emitted; and a third step of computing assay values based on the light amounts of delayed fluorescence, respectively measured in the first step and the second step, and determining a comparison value of the assay
    Type: Grant
    Filed: December 16, 2004
    Date of Patent: September 20, 2016
    Assignee: HAMAMATSU PHOTONICS K.K.
    Inventors: Masakazu Katsumata, Hiroshi Tsuchiya, Takashi Koike, Masataka Nishikawa
  • Patent number: 9399788
    Abstract: The present disclosure describes a method capable of easily and rapidly inspecting the susceptibility of bacteria or fungi to an antimicrobial drug. The inspection method of the present disclosure is a method for inspecting susceptibility of bacteria or fungi to an antimicrobial drug using a micro-device having flow channels, including: incubating a mixture of the antimicrobial drug and a suspension to be inspected in the flow channels of the micro-device; and detecting bacteria or fungi derived from the suspension to be inspected in an observation area of the flow channels of the micro-device. The detecting step can be performed by detecting an increase or decrease in the number of or a change in shape of bacteria or fungi derived from the suspension to be inspected in the observation area by a microscope or the like.
    Type: Grant
    Filed: August 31, 2012
    Date of Patent: July 26, 2016
    Assignees: Osaka University, FUKOKU Co., Ltd.
    Inventors: Yoshimi Matsumoto, Kohei Hayama, Shouichi Sakakihara, Kunihiko Nishino, Akihito Yamaguchi, Hiroyuki Noji, Ryota Iino
  • Patent number: 9334479
    Abstract: The invention relates to human liver pluripotent progenitor cell lines which express hepatic cell markers such as albumin and ?-fetoprotein and do not express some of the markers which are typical of oval stem cells. Also disclosed is a method of isolating the cell lines of the invention, methods for differentiating said cells into a plurality of different cell lineages, methods for conditional immortalization and metabolic selection of said cells, as well as the use of the cell lines of the invention for preparing a medicament with osteogenic differentiation activity or liver injury regeneration activity.
    Type: Grant
    Filed: May 24, 2006
    Date of Patent: May 10, 2016
    Assignee: FRESENIUS MEDICAL CARE DEUTSCHLAND GMBH
    Inventors: Maria Beatriz Herrera Sanchez, Benedetta Bussolati, Giovanni Camussi, Stefano Buttiglieri
  • Patent number: 9290790
    Abstract: This invention relates to an antimicrobial susceptibility test made upon pure microbial cultures—a laboratory culture containing a single species of organism—or directly from biological samples uncultured. Some preferred embodiments do not require the use a pure culture obtained from a biological product which would otherwise take at least 24 hours for the microorganisms to grow—so, is performed an antimicrobial susceptibility test directly from uncultured biological samples like urine, blood cultures or water by fluorescence analysis.
    Type: Grant
    Filed: June 4, 2012
    Date of Patent: March 22, 2016
    Assignee: UNIVERSIDADE DO PORTO
    Inventors: Cidália Irene Azevedo Pina Vaz, Acácio Agostinho Goncalves Rodrigues, Isabel Cristina Santos Silva De Faria Ramos Antunes, Ana Sofia Quinta E Costa De Oliveira Morais, Ana Teresa Pinto E Silva, Ana Isabel Silva Dias, Rita Daniela Teixeira Dos Santos
  • Patent number: 9241971
    Abstract: Disclosed herein are methods, compositions, and kits for treating a skin condition caused by a bacterial infection at a skin depth with a topical ointment comprising vancomycin hydrochloride. Also disclosed herein are methods, compositions, and kits for testing susceptibility of vancomycin for treating a bacterial infection at a skin depth, and of optimizing a topical ointment therapeutic regimen.
    Type: Grant
    Filed: September 24, 2014
    Date of Patent: January 26, 2016
    Assignee: KUROBE, LLC
    Inventors: Barry Butler, William Stringer, Colin Butler, Jeremy Brace, Ronil Patel
  • Patent number: 9233142
    Abstract: (Problems) To provide a therapeutic agent for infections comprising granulysin as an active ingredient which has little side effect and no cytotoxicity and to which bacteria can hardly acquire resistance, and a treatment method using the same. (Means for Solving Problems) The present invention provides a therapeutic agent for infections comprising as active ingredient: 15K granulysin, a combination of 15K granulysin and 15K granulysin in vivo expression vector, a combination of 15K granulysin and at least one interleukin selected from IL-6, IL-23 or IL-27, a combination of 15K granulysin in vivo expression vector and at least one interleukin selected from IL-6, IL-23 or IL-27, or a combination of 15K granulysin in vivo expression vector and HSP65DNA and IL-12DNA in vivo expression vector, which enhances killing effects on bacteria and has less side effect, and to which bacteria can hardly acquire resistance, and a treatment method using the same.
    Type: Grant
    Filed: June 2, 2014
    Date of Patent: January 12, 2016
    Assignees: National Hospital Organization Kinki-chuo Chest Medical center, AZBIO Corporation
    Inventors: Masaji Okada, Yasushi Takamori, Kazuyuki Ogawa, Kinya Nagata
  • Patent number: 9206463
    Abstract: The disclosure provides for a method of testing a disinfectant or antibiotic using phenanthridium derivatives with a 2+ charge or higher.
    Type: Grant
    Filed: May 23, 2014
    Date of Patent: December 8, 2015
    Assignee: Montana State University
    Inventors: Andreas Nocker, Anne K. Camper
  • Patent number: 9133498
    Abstract: A testing method is disclosed. The testing method includes: providing a mixture solution of a gelling agent and a microbe to a gelling device; solidifying the mixture solution to form a solid thin film in which the microbe is immobilized; supplying a bioactive agent to the solid thin film and allowing the bioactive agent to diffuse into the solid thin film; and imaging the individual responses of the single microbial cells to the bioactive agent, and determining the minimum inhibitory concentration (MIC) of the bioactive agent based on the analysis of the images to obtain AST results.
    Type: Grant
    Filed: February 1, 2013
    Date of Patent: September 15, 2015
    Assignees: SNU R & DB FOUNDATION, QUANTAMATRIX
    Inventors: Sunghoon Kwon, Yong-Gyun Jung, Jung Il Choi, Hun Jong Na
  • Patent number: 9051594
    Abstract: Microorganisms, particularly bacteria, are identified and characterized on the basis of a mass spectrometric measurement of their protein profiles with ionization by matrix-assisted laser desorption. In order to measure the microbial resistance to antibiotics, the protein profiles of microorganisms are measured after cultivation for a short time duration in nutrient media containing the antibiotics.
    Type: Grant
    Filed: October 10, 2013
    Date of Patent: June 9, 2015
    Inventors: Vadim Markovich Govorun, Jochen Franzen
  • Publication number: 20150148359
    Abstract: Uses of a compound of any of Formulas I-VI as a cytotoxic inhibitor of undifferentiated cells are disclosed herein, as well as pharmaceutical compositions comprising a compound of any of Formulas I-VI, and methods for identifying a lead candidate for inhibiting undifferentiated cells. Further disclosed are uses of an SCD-1 inhibitor as a cytotoxic inhibitor of undifferentiated cells.
    Type: Application
    Filed: May 22, 2013
    Publication date: May 28, 2015
    Inventors: Uri Ben-David, Nissim Benvenisty, Payal Arora, Qing-Fen Gan, Ralph J. Garippa
  • Publication number: 20150147774
    Abstract: The present disclosure provides an isolated linear expression cassette. The disclosed cassette comprises a bidirectional promoter; a first gene and a second gene that the genes are respectively and operably linked to one end of the bidirectional promoter; a terminator located immediately next to end of each gene; a 5?-homogolgous region to genomic context of interest and a 3?-homogolgous region to genomic context of interest respectively flanking 5? end and 3? end of the cassette; and a dominant selection marker residing within the construct and being arranged in between the flanking 5?-homogolgous region to genomic context of interest and a 3?-homogolgous region to genomic context of interest. Preferably, the cassette is capable of being integrated into genome of transformed yeasts upon being transported into the transformed cell.
    Type: Application
    Filed: November 24, 2014
    Publication date: May 28, 2015
    Inventors: Jifeng YUAN, Chi Bun CHING
  • Publication number: 20150140551
    Abstract: A method for investigating one or a plurality of phase objects is described, in which a grid made up of elements is used, which is illuminated with light of a light source, the coherence length of which is larger than the average spacing of adjacent elements of the grid. A diffraction image of the illuminating light scattered on the grid is generated, whereby the one or the plurality of phase objects are placed in the light path between the light source and the grid and/or in the light path of the illuminating light scattered on the grid. At least a part of the diffraction image is detected by an optical sensor directly or after interaction with further optical components and converted into a signal. The signal is analysed further in order to ascertain information relating to the one or plurality of phase objects therefrom. A corresponding device is likewise described.
    Type: Application
    Filed: November 12, 2014
    Publication date: May 21, 2015
    Inventors: Philipp Paulitschke, Joachim Radler
  • Publication number: 20150133450
    Abstract: The present invention relates to compositions and methods for cancer treatment comprising compounds of Formulae I, II, and III. In some aspects, the invention relates to the treatment of B-cell Lymphoma or other hematopoietic cancers. In other aspects, the invention provides methods for treating particular types of hematopoietic cancers, such as, for example, B-cell lymphoma, using a combination of one or more compounds of Formulae I, II, and III. Combination therapy with, for example, 26S proteasome inhibitors, such as, for example, Bortezomib, are also included. In another aspect the present invention relates to autoimmune treatment with compounds of Formulae I, II, and III. In another aspect, this invention relates to methods for identifying compounds, for example, compounds of the BH3 mimic class, that have in vitro properties that predict in vivo efficacy against B-cell lymphoma tumors and other cancers as well as autoimmune disease.
    Type: Application
    Filed: June 20, 2013
    Publication date: May 14, 2015
    Inventors: Michael H. Cardone, David Richard
  • Publication number: 20150135341
    Abstract: Provided herein are an isolated or enriched population of tumor initiating cells derived from normal cells, cells susceptible to neoplasia, or neoplastic cells. Methods of use of the cells for screening for anti-hyperproliferative agents, and use of the cells for animal models of hyperproliferative disorders including metastatic cancer, diagnostic methods, and therapeutic methods are provided.
    Type: Application
    Filed: January 31, 2014
    Publication date: May 14, 2015
    Applicant: GENESYS RESEARCH INSTITUTE
    Inventor: Lynn Hlatky
  • Publication number: 20150087011
    Abstract: A device for analysis and monitoring of toxicity in waters is described. The device is specifically devised for determining toxicity in waters in several samples at the same time and in quick time with a high degree of accuracy and precision.
    Type: Application
    Filed: May 14, 2013
    Publication date: March 26, 2015
    Inventor: Pompeo Moscetta
  • Publication number: 20150087013
    Abstract: A novel gene encoding P. pastoris orotate-phosphoribosyl transferase (URA5) is disclosed. Methods for producing and selecting yeast strains capable of stable genetic integration of heterologous sequences into the host genome are also provided.
    Type: Application
    Filed: December 11, 2014
    Publication date: March 26, 2015
    Inventor: Juergen H. Nett
  • Publication number: 20150087012
    Abstract: A method for quantitatively evaluating the antimicrobial activity of an antimicrobial sample using a disc diffusion method, and a measurement system using the method are provided. The method comprises: (a) measuring a maximum inhibition zone width (IZWmax) for a first sample, in which a colony is expected not to be observed, using a disc diffusion method, and measuring a colony forming unit (CFU0) value for the first sample using a colony counting method; (b) measuring a microbial growth inhibition zone width (IZWi) and a colony forming unit (CFUi) value for a second sample having any antimicrobial activity; (c) calculating ? value and ? value based on the results of steps (a) and (b) using equations (10) and (20); (d) measuring a microbial growth inhibition zone width (IZWx) for a measurement sample; and (e) calculating a quantitative antimicrobial activity value (?) using equation (30).
    Type: Application
    Filed: September 26, 2014
    Publication date: March 26, 2015
    Inventors: Jung Ho HWANG, Yun Haeng JOE
  • Patent number: 8986946
    Abstract: The invention relates to a method for real-time detection of viable microorganisms comprising: a. addition of a cell-permeable, phototautomeric compound to a micro-organism or other living cell; and b. measuring the fluorescent emission of said phototautomeric compound. Preferably the phototautomeric compound is salicylic acid, 2-hydroxy-1-naphtoic acid or 1-hydroxy-2-naphtoic acid. Further, the assay can he used to assess the antibiotic effect of a test compound. This test can be used as a high—throughput screening for compounds with antibiotic activity. Also part of the invention is the use of a cell permeable phototautomeric compound in a method for determining the viability of micro-organisms and for assessing the antibiotic effect of a test compound.
    Type: Grant
    Filed: December 19, 2008
    Date of Patent: March 24, 2015
    Assignee: Nederlandse Organisatie Voor Toegepast-Natuurwetenschappelijk Onderzoek Tno
    Inventors: Remco Kort, Frank Henri Johan Schuren, Roy Christiaan Montijn
  • Publication number: 20150079626
    Abstract: The present invention relates to a method of obtaining a cell population containing cancer stem cells, which comprises culturing iPS cells in the presence of culture supernatant of cancer cells.
    Type: Application
    Filed: March 18, 2014
    Publication date: March 19, 2015
    Applicants: LSIP, LLC, National University Corporation Okayama University
    Inventors: Masaharu SENO, Akifumi MIZUTANI, Tomonari KASAI
  • Publication number: 20150079584
    Abstract: A three dimensional cell culture and bioreactor system is provided. The system comprises one or more cell culture chamber. Each cell culture chamber comprises an inlet port and an outlet port in fluid communication with the cell culture chamber. The cell culture chambers may be segregated or in fluid communication with one another. The systems may be used to conduct drug efficacy test, isolate certain cell types from a complex tissue sample of multiple cell types, allow for the ex vivo culturing of patient tissue samples to help guide the course of treatment, and conduct co-culture experiments.
    Type: Application
    Filed: March 17, 2014
    Publication date: March 19, 2015
    Inventors: Matthew R. Gevaert, David E. Orr, Hal Crosswell
  • Publication number: 20150071933
    Abstract: Annexin A3 (ANAX3) is utilized as a biomarker for the diagnosis and prognosis of hepatocellular carcinoma (HCC) and the utilization of a monoclonal antibody against ANXA3 or antisense polynucleotide against ANXA3 mRNA for the suppression or treatment of HCC, alone or in combination with other HCC treatment. Monoclonal antibody against ANXA3 can be administered for the suppression of tumor growth, metastasis, and chemoresistance.
    Type: Application
    Filed: September 12, 2014
    Publication date: March 12, 2015
    Inventors: Stephanie Kwai Yee Ma, Man Tong, Xin-Yuan Guan
  • Publication number: 20150072373
    Abstract: System and Method for measuring the growth of a bacterial culture and its response to one or more antimicrobials using measurement of mass of individual microbes. Methods include periodic sampling, determining change in mass and concentration, and comparing growth rates of cultures in nutrient broth vs. mixtures containing various antibiotic mixtures. A number of antimicrobials can be compared in one measurement by multiplexing or using multiple sensors to measure in parallel. Growth and antibiotic efficacy can be assessed at low concentrations at the onset of growth, typically within 1 to 2 hours.
    Type: Application
    Filed: September 8, 2014
    Publication date: March 12, 2015
    Applicant: AFFINITY BIOSENSORS, LLC
    Inventors: Ken Babcock, Cynthia Schneider
  • Publication number: 20150056648
    Abstract: Disclosed are methods of determining biocide efficacy using flow cytometry. The methods can be used to determine the synergy between at least two biocides.
    Type: Application
    Filed: August 22, 2014
    Publication date: February 26, 2015
    Inventors: Timothy J. Tidwell, Zachary Richard Broussard
  • Publication number: 20150056169
    Abstract: Despite efforts of eradication and sanitation, Phytophthora ramorum persists in the United States and has been a problem in commercial nurseries since 2001. Detection in a nursery immediately causes the nursery to be under quarantine until the P. ramorum can be proven to be eradicated. Treatment with the biological control agent Trichoderma asperellum showed promise in laboratory results to remediate P. ramorum-infested potting soil. Sixteen T. asperellum isolates were screened, found to have variable mycoparasitic activities, and further tested in natural field settings. Field plots having P. ramorum-infested soil were treated with T. asperellum, a chemical and two different commercial biological control products and compared to non-treated infested soil. After 8 weeks, P. ramorum populations were not detected in the soil treated with T. asperellum isolate 04-22 showing that T. asperellum isolate 04-22 could remediate soils infested with P. ramorum under natural field conditions.
    Type: Application
    Filed: August 20, 2013
    Publication date: February 26, 2015
    Inventors: Timothy L. Widmer, Gary J. Samuels
  • Patent number: 8962550
    Abstract: A system, a composition, a method and a kit for identifying anti-bacterial agents are provided. The invention described herein is useful in identifying inhibitors of any bacterial stress response. Moreover, the invention can be applied to any sRNA and its target, any transcription factor and its target, and any transcription factor/sRNA pair (i.e., a transcription factor that regulates a sRNA). In particular, the present invention provides a system, a composition, a method and a kit for the identification of cyclic peptides that block the ?E pathway in Escherichia coli.
    Type: Grant
    Filed: December 16, 2011
    Date of Patent: February 24, 2015
    Assignee: The Penn State Research Foundation
    Inventors: Sarah Ellen Ades, Kenneth Charles Keiler