Streptococcus; Staphylococcus Patents (Class 435/36)
  • Patent number: 9017963
    Abstract: Described herein are methods of detecting a wound infection and for detecting the presence or absence of microorganisms, for example, wound pathogens in a sample, by contacting a sample with an enzyme produced and/or secreted by the bacteria, and detecting modification or the absence of modification of the substrate, as an indicator of the presence or absence of the enzyme in the sample. The present invention also features a biosensor for detecting the presence or absence of bacteria in a sample.
    Type: Grant
    Filed: January 31, 2003
    Date of Patent: April 28, 2015
    Assignee: Woundchek Laboratories (US), Inc.
    Inventors: Mitchell C. Sanders, Adrian M. Lowe, Maureen A. Hamilton, Gerard J. Colpas
  • Publication number: 20150111243
    Abstract: The invention provides vesicles that may be used in diagnosing infection, especially with infection by bacteria such as Staphylococcus aureus and Pseudomonas aeruginosa. The vesicles can be used in wound dressings.
    Type: Application
    Filed: July 27, 2012
    Publication date: April 23, 2015
    Applicant: The University of Bath
    Inventors: Andrew Tobias Jenkins, Naig Tun Thet, June Mercer-Chalmers
  • Publication number: 20150104787
    Abstract: Methods and systems are provided for packaging reporter nucleic acid molecules into non-replicative transduction particles for use as reporter molecules. The non-replicative transduction particles can be constructed from viruses and use viral transduction and replication systems. The reporter nucleic acid molecules include a reporter gene, such as a reporter molecule or selectable marker, for detecting target genes or cells. Methods and systems are provided for detection of cells and target nucleic acid molecules using the non-replicative transduction particles as reporter molecules.
    Type: Application
    Filed: November 21, 2014
    Publication date: April 16, 2015
    Inventors: Diego Ariel Rey, Nikol DeForest, Heather Cox, Soni Shukla
  • Publication number: 20150099273
    Abstract: The present invention relates to a bacterial cell with texturizing property, starter cultures comprising the cell, and dairy products fermented with the starter culture.
    Type: Application
    Filed: September 29, 2014
    Publication date: April 9, 2015
    Applicant: CHR. HANSEN A/S
    Inventors: Thomas JANZEN, Ditte Ellegaard CHRISTIANSEN
  • Publication number: 20150024377
    Abstract: The present invention, in part, relates to an apparatus having a single-use, normally-closed fluidic valve that is initially maintained in the closed position by a valve element bonded to an adhesive coating. The valve is opened using a magnetic force. The valve element includes a magnetic material or metal. In some examples, the valve is opened by bringing a magnet in proximity to the valve element to provide a magnetic force that delaminates the valve element from the adhesive coating. In particular, the apparatus can be useful for on-chip amplification and/or detection of various targets, including biological targets and any amplifiable targets. Such apparatuses and methods are useful for in-field or real-time detection of targets, especially in limited resource settings.
    Type: Application
    Filed: January 16, 2014
    Publication date: January 22, 2015
    Applicant: Sandia Corporation
    Inventors: Thayne L. Edwards, Jason C. Harper
  • Publication number: 20150004644
    Abstract: The present invention relates to a method for studying a sample containing a bacterial population of Staphylococcus aureus with a specific mass spectrometry technique allowing specific detection on the obtained mass spectrum of the presence or of the absence of a peak at an m/z value of 3005±5 Th Thomson or at 3035±5 Th Thomson, and accordingly, to the issuance of a decision conditioned by this result.
    Type: Application
    Filed: November 6, 2012
    Publication date: January 1, 2015
    Inventors: Francois Vandenesch, Olivier Dauwalder, Jean-Philippe Charrier, Martin Welker
  • Publication number: 20140370542
    Abstract: The present invention is an apparatus for detecting the presence, quantity and identity of one or more microorganisms in a sample and a method for using the same. The apparatus is composed of one or more chambers and a sensing element for sensing microorganisms. In particular embodiments, the sensing element is an array of chemoresponsive dyes deposited on a substrate in a predetermined pattern combination, wherein the combination of the dyes have a distinct and direct spectroscopic, transmission, or reflectance response to distinct analytes produced by the microorganism which is indicative of the presence, quantity and identity of the microorganism.
    Type: Application
    Filed: August 28, 2014
    Publication date: December 18, 2014
    Inventors: Kenneth S. Suslick, Matthew J. Placek, William B. McNamara, III, Avijit Sen, James R. Carey, Jennifer B. Wilson, Crystal K. Keso
  • Publication number: 20140349325
    Abstract: A method and medium for detecting the presence or absence of a targeted strain of antibiotic resistant pathogenic staphylococci in a test sample is provided. The method includes the steps of: a) providing a test medium having at least one hydrolysable substrate, which substrate is operable to promote the growth of the targeted strain of antibiotic resistant pathogenic staphylococci and to produce a detectable signal when the hydrolyzable substrate is metabolized by the targeted strain of antibiotic resistant pathogenic staphylococci; b) forming a mixture of the test sample and hydrated test medium in the test tube; c) incubating the mixture at temperatures in the range of about 20° C. to about 35° C.; and d) detecting the presence or absence of the targeted strain of antibiotic resistant pathogenic staphylococci in the test sample by the presence or absence of the detectable signal in the mixture in the tube.
    Type: Application
    Filed: November 10, 2011
    Publication date: November 27, 2014
    Applicant: Pilots Point LLC
    Inventor: Stephen C. Edberg
  • Patent number: 8889351
    Abstract: The disclosure provides articles and methods useful for detecting a discrete source of DNase activity. DNase-producing microorganisms can be detected. The device can further include selective agents and/or indicators to differentiate groups or species microorganisms. Methods of use include detecting or enumerating DNase-producing microorganisms.
    Type: Grant
    Filed: February 22, 2010
    Date of Patent: November 18, 2014
    Assignee: Innovative Properties Company
    Inventors: Patrick A. Mach, Michelle L. Rosauer, Michael E. Hughes
  • Publication number: 20140336080
    Abstract: The present invention relates to a method of diagnosing an existing or developing acute pneumonia induced by a microorganism by detecting one or more volatile organic compound(s) in a subject's sample and the use of one or more volatile organic compound(s) for the detection of Staphylococcus aureus, Pseudomonas aeruginosa, Streptococcus pneumoniae or Haemophilus influenza and optionally opportunistic pathogen Candida albicans.
    Type: Application
    Filed: December 3, 2012
    Publication date: November 13, 2014
    Applicant: MEON Medical Solutions GmbH & Co.KG
    Inventors: Horst Ruether, Anton Amann
  • Publication number: 20140335559
    Abstract: The present invention relates to a method for the semi-quantitative determination of cariogenic bacteria in a sample of saliva and/or plaque, in which the microorganisms contained in a sample of plaque or saliva are brought into contact with a carbon source that is fermented to acid by the cariogenic bacteria. The microorganisms are then incubated under conditions which make possible selective acid formation by the cariogenic bacteria. The acid formation is then detected by determining the pH at least once within a period of 12 hours after adding the carbon source, wherein the cariogenic bacteria are determined semi-quantitatively in the sample by comparison of the pH with at least one reference value. In addition, the invention provides kits for carrying out such methods.
    Type: Application
    Filed: May 15, 2014
    Publication date: November 13, 2014
    Applicant: Ivoclar Vivadent AG
    Inventors: Manuela Bischof, Carlo Bolis, Urs Lendenmann, Volker Rheinberger
  • Publication number: 20140322715
    Abstract: The invention comprises a method to determine the chance of a successful pregnancy based on the ratio of said ratio indicated as a formula that uses the presence of Lactobacillus and Staphylococcus bacteria in relation to the total amount of bacteria in a sample of a subject, preferably a urine sample. Also comprised in the invention is a kit, preferably a qPCR kit, for performing the method of the invention and outputting the result. Such a method and kit are particularly advantageous for predicting the chance of a successful pregnancy in subjects undergoing or eligible for an artificial insemination method such as IVF or ICSI.
    Type: Application
    Filed: August 12, 2011
    Publication date: October 30, 2014
    Applicant: Erasmus University Medical Center Rotterdam
    Inventors: Dirk Jan Kok, Jozef Stephanus Elisabeth Laven, Delshad Mama Maghdid, Nicole Geertje Maria Beckers
  • Publication number: 20140322749
    Abstract: Provided is a method for measuring a color change of an oxidation-reduction indicator, which method is applicable to measurement of the cariogenic bacterial count and the like. A color change of an oxidation-reduction indicator is measured by a method for measuring a color change of an oxidation-reduction indicator, the method comprising reacting a test reagent with a test sample and measuring a color change, wherein the test reagent contains an oxidation-reduction indicator, an oxidation-reduction promoter, and a halogen salt.
    Type: Application
    Filed: August 17, 2012
    Publication date: October 30, 2014
    Applicant: ARKRAY, INC.
    Inventors: Eiji Nishinaga, Naho Suzuki, Takashi Hama, Isao Fukuta
  • Publication number: 20140295473
    Abstract: A method of and test mixture for detecting the presence or absence of a target microbe in a sample is provided. The method includes the steps of: a) providing a test mixture that includes micro particles, a substrate in an amount that is sufficient to support log phase growth of the target microbe until a detectable characteristic signal is produced in the test mixture and sample admixture, and an amount of vitamin, amino acid, element and salt ingredients; b) combining the powdered test mixture and sample to form the admixture; and c) detecting the presence or absence of target microbes in the sample based on the presence or absence of the detectable characteristic.
    Type: Application
    Filed: June 13, 2014
    Publication date: October 2, 2014
    Inventor: Stephen C. Edberg
  • Publication number: 20140287455
    Abstract: An evaluation kit for a microorganism detecting device includes an immobilized microorganism. A manufacturing method for an evaluation kit for a microorganism detecting device includes immobilizing a microorganism. An evaluating method for the microorganism detecting device includes detecting an immobilized microorganism by the microorganism detecting device. The immobilized microorganisms may be dispersed in a solvent. The solvent may be water. The immobilized microorganisms may be immobilized with an aldehyde. The aldehyde may be formaldehyde. The immobilized microorganisms emit, for example, fluorescent light.
    Type: Application
    Filed: March 20, 2014
    Publication date: September 25, 2014
    Applicant: Azbil Corporation
    Inventor: Kanami IRIE
  • Publication number: 20140271490
    Abstract: The present invention relates to bacteria-targeted contrast agents for magnetic resonance imaging (MRI). In particular, the present invention relates to bacteria targeted MRI contrast agents that can be used to detect bacteria in vivo or in vitro. In certain embodiments, the contrast agents comprise a metal chelate conjugated to at least two Zn-dipicolylamine groups.
    Type: Application
    Filed: March 6, 2014
    Publication date: September 18, 2014
    Applicant: Northwestern University
    Inventors: Lauren M. Matosziuk, Allison S. Harney, Keith W. MacRenaris, Thomas J. Meade
  • Publication number: 20140234831
    Abstract: Provided herein is a composition for detection of a microbial product in a sample comprising a polydiacetylene (PDA) and a packaging polymer, wherein the sample contacts the PDA and a change of PDA color indicates detection. Also provided herein are methods of making a packaging material comprising a PDA and methods of using the composition for detection of a microbial product in a sample.
    Type: Application
    Filed: September 28, 2012
    Publication date: August 21, 2014
    Inventor: Richard Awdeh
  • Patent number: 8765400
    Abstract: The present invention relates to a method for the semi-quantitative determination of cariogenic bacteria in a sample of saliva and/or plaque, in which the microorganisms contained in a sample of plaque or saliva are brought into contact with a carbon source that is fermented to acid by the cariogenic bacteria. The microorganisms are then incubated under conditions which make possible selective acid formation by the cariogenic bacteria. The acid formation is then detected by determining the pH at least once within a period of 12 hours after adding the carbon source, wherein the cariogenic bacteria are determined semi-quantitatively in the sample by comparison of the pH with at least one reference value. In addition, the invention provides kits for carrying out such methods.
    Type: Grant
    Filed: July 12, 2011
    Date of Patent: July 1, 2014
    Assignee: Ivoclar Vivadent AG
    Inventors: Manuela Bischof, Carlo Bolis, Urs Lendenmann, Volker Rheinberger
  • Patent number: 8741597
    Abstract: A reaction medium for detecting and/or identifying Staphylococcus aureus bacteria, comprising a combination of two enzymatic substrates for alpha-glucosidase.
    Type: Grant
    Filed: October 7, 2009
    Date of Patent: June 3, 2014
    Assignee: Biomérieux
    Inventors: Sylvain Orenga, Denis Robichon
  • Patent number: 8697053
    Abstract: The invention includes methods and compositions for treating an animal to inhibit the incidence and growth of E. coli O157:H7 and other pathogenic bacteria. The treatment method comprises administering a therapeutically effective amount of Lactobacillus acidophilus or one or a combination of a number of other probiotic bacteria to an animal. An alternative treatment method comprises administering a therapeutically effective amount of a lactic acid producing bacterium such as Lactobacillus acidophilus in combination with a lactate utilizing bacterium such as Propionibacterium freudenreichii.
    Type: Grant
    Filed: April 9, 2009
    Date of Patent: April 15, 2014
    Assignee: Nutrition Physiology Company, LLC
    Inventors: Bryan E. Garner, Douglas R. Ware
  • Publication number: 20140051089
    Abstract: Described herein are methods of detecting a wound infection and for detecting the presence or absence of microorganisms, for example, wound pathogens in a sample, by contacting a sample with an enzyme produced and/or secreted by the bacteria, and detecting modification or the absence of modification of the substrate, as an indicator of the presence or absence of the enzyme in the sample. The present invention also features a biosensor for detecting the presence or absence of bacteria in a sample.
    Type: Application
    Filed: July 23, 2013
    Publication date: February 20, 2014
    Applicant: Systagenix Wound Management (US), Inc.
    Inventors: Mitchell C. Sanders, Adrian M. Lowe, Maureen A. Hamilton, Gerard J. Colpas
  • Publication number: 20130344521
    Abstract: A method of and test mixture for detecting the presence or absence of a target microbe in a sample is provided. The method includes the steps of: a) providing a test mixture that includes micro particles, a substrate in an amount that is sufficient to support log phase growth of the target microbe until a detectable characteristic signal is produced in the test mixture and sample admixture, and an amount of vitamin, amino acid, element and salt ingredients; b) combining the powdered test mixture and sample to form the admixture; and c) detecting the presence or absence of target microbes in the sample based on the presence or absence of the detectable characteristic.
    Type: Application
    Filed: July 16, 2013
    Publication date: December 26, 2013
    Inventor: Stephen C. Edberg
  • Publication number: 20130330710
    Abstract: The present disclosure relates to biophotonic sensors. An example of a biophotonic sensor may be an apparatus for analyzing a sample. The apparatus may include a substrate, aperiodic nanostructured protrusions disposed on the substrate, and a silk material deposited between the protrusions.
    Type: Application
    Filed: July 30, 2011
    Publication date: December 12, 2013
    Applicants: TRUSTEES OF BOSTON UNIVERSITY, TUFTS UNIVERSITY
    Inventors: Fiorenzo Omenetto, David Kaplan, Jason Amsden, Luca Dal Negro
  • Publication number: 20130309168
    Abstract: The invention provides a family of agents that target bacterial infection, which can be used as imaging agents or therapeutic agents. The agents can be used to image sites of bacterial infection as well as other physiological processes in a subject.
    Type: Application
    Filed: March 15, 2013
    Publication date: November 21, 2013
    Applicant: VisEn Medical, Inc.
    Inventor: VisEn Medical, Inc.
  • Patent number: 8569010
    Abstract: The invention mainly relates to the mass spectrometric identification of pathogens in blood cultures from blood-stream infections (septicemia). The invention provides a method with which microbial pathogens can be separated in purified form from blood after a relatively brief cultivation in a blood culture flask, without any interfering human proteins or any residual fractions of blood particles such as erythrocytes and leukocytes, and can be directly identified by mass spectrometric measurement of their protein profiles. The method is based on the use of relatively strong tensides to destroy the blood particles by dissolving the weak cell membranes and most of the internal structures of the blood particles; in spite of the fact that tensides are regarded as strong ionization inhibitors in MALDI and other ionization processes required for mass spectrometric measurements.
    Type: Grant
    Filed: July 14, 2010
    Date of Patent: October 29, 2013
    Assignee: Bruker Daltonik GmbH
    Inventor: Thomas Maier
  • Patent number: 8557537
    Abstract: The present invention relates to a method for the production of an overproducing Staphylococcus aureus strain comprising: (a) culturing a Staphylococcus aureus strain on a culture medium M1, M2 or M3, (b) optionally, recovering the strains thus produced from the culture medium, and also to the use of said strains for the production of polysaccharides.
    Type: Grant
    Filed: September 13, 2010
    Date of Patent: October 15, 2013
    Assignee: Sanofi Pasteur SA
    Inventors: Bachra Rokbi, Celine Lafont
  • Patent number: 8546103
    Abstract: A presence/absence test for Staphylococcus aureus (S. aureus) involves placing a first generation test sample in a solution that will clot in the presence of S. aureus. The solution contains components that will selectively grow S. aureus and also contains clotting factors that will react with S. aureus, if S. aureus is present in the sample, to clot the solution. Examples of specimen samples that can be tested include nasal swabs and lesion swabs, among others. The test can also be modified to detect the presence or absence of methicillin resistant S. Aureus (MRSA). The addition of micro particles having a size in the range of about 0.1 micron to about 1.0 mm provides localities where the bacteria agglomerate, thereby significantly decreasing the clotting time, and providing a significantly stronger clot. The micro particles can be used in other bacteria tests to accelerate the production of an end result.
    Type: Grant
    Filed: June 25, 2010
    Date of Patent: October 1, 2013
    Assignee: Pilots Point, LLC
    Inventor: Stephen C. Edberg
  • Publication number: 20130216576
    Abstract: Disclosed is a new and emerging serotype of Streptococcus pneumoniae designated serotype 6D, and assays and monoclonal antibodies useful in identifying same. Also disclosed is a novel pneumococcal polysaccharide with the repeating unit?2) glucose 1 (1?3) glucose 2 (1?3) rhamnose (1?4) ribitol (5?phosphate. This new serotype may be included in pneumococcal vaccines.
    Type: Application
    Filed: April 19, 2013
    Publication date: August 22, 2013
    Applicant: UAB Research Foundation
    Inventor: UAB Research Foundation
  • Patent number: 8497086
    Abstract: A reaction medium for detecting and/or identifying methicillin-resistant Staphylococcus aureus (MRSA) bacteria includes a chromogenic substrate, a first antibiotic that belongs to the cephalosporin family and a second antibiotic that belongs to the aminoglycoside family.
    Type: Grant
    Filed: July 20, 2010
    Date of Patent: July 30, 2013
    Assignee: Biomereux
    Inventors: Jean-Marc Roche, Gilles Zambardi
  • Publication number: 20130178834
    Abstract: Extracorporeal systems and methods for treating blood-borne diseases in a subject or for developing drugs to treat blood-borne diseases include various environmental and treatment modules that can be tailored to a specific disease or infection. In certain embodiments of the systems and methods, a blood sample is treated with cold plasma and optionally with hydrostatic pressure, a pulsed electrical field, a pharmaceutical agent, microwave, centrifugation, sonification, radiation, or a combination thereof, under environmental conditions that are effective for the treatment.
    Type: Application
    Filed: January 9, 2013
    Publication date: July 11, 2013
    Applicant: SOMERSET GROUP ENTERPRISES, INC.
    Inventor: SOMERSET GROUP ENTERPRISES, INC.
  • Patent number: 8481281
    Abstract: The present invention is based on the discovery that drug resistance in microorganisms can be rapidly and accurately determined using mass spectrometry. A mass spectrum of an intact microorganism or one or more isolated biomarkers from the microorganism grown in drug containing, isotopically-labeled media is compared with a mass spectrum of the intact microorganism or one or more isolated biomarkers from the microorganism grown in non-labeled media without the drug present. Drug resistance is determined by predicting and detecting a characteristic mass shift of one or more biomarkers using algorithms. The characteristic mass shift is indicative that the microorganism is growing in the presence of the drug and incorporating the isotopic label into the one or more biomarkers, resulting in change in mass.
    Type: Grant
    Filed: February 17, 2011
    Date of Patent: July 9, 2013
    Assignee: The Johns Hopkins University
    Inventors: Plamen A. Demirev, Nathan A. Hagan, Miquel D. Antoine, Jeffrey S. Lin, Andrew B. Feldman
  • Publication number: 20130095521
    Abstract: The present invention relates to a method for testing the sterility of a plant by checking for the presence and identifying at least one microorganism.
    Type: Application
    Filed: October 12, 2012
    Publication date: April 18, 2013
    Applicant: Fresenius Kabi Anti-Infectives S.r.l.
    Inventor: Lidia de Rigo
  • Patent number: 8420347
    Abstract: A test mixture for detecting the presence or absence of Methicillin Resistant Staphylococcus Aureus (“MRSA”) directly in a first generation biological specimen, the test mixture including: a) an amount of amino acids; b) an amount of nitrogen sources; c) an amount of salts; d) an amount of vitamins; e) an amount of calcium; f) an amount of coagulase substrates which will clot the test mixture in the presence of S. aureus in the first generation biological specimen; and g) an amount of a MecA gene inducer effective to kill any Methicillin Susceptible Staphylococcus Aureus (“MSSA”) in the first generation biological specimen.
    Type: Grant
    Filed: July 12, 2011
    Date of Patent: April 16, 2013
    Assignee: Pilots Point LLC
    Inventor: Stephen C. Edberg
  • Patent number: 8415115
    Abstract: A reaction medium for detecting and/or identifying Methicillin-resistant Staphylococcus aureus (MRSA) bacteria, comprising a predetermined combination of two antibiotics, a first antibiotic which belongs to the cephalosporin family and a second antibiotic, said first and second antibiotics each being at a sub-inhibitory concentration.
    Type: Grant
    Filed: October 7, 2009
    Date of Patent: April 9, 2013
    Assignee: Biomerieux
    Inventors: Sylvain Orenga, Denis Robichon, Gilles Zambardi
  • Publication number: 20130084588
    Abstract: The invention provides methods of detecting bacteria in fluids, including blood, platelets and other blood products for transfusion, and urine. The methods are based on lysing the bacteria to release ATP and detecting the ATP. Eukaryotic cell contamination is a problem to be overcome, because eukaryotic cell contain large amounts of ATP. Thus, some of the methods involve separating intact eukaryotic cells (e.g., platelets) from intact bacterial cells before lysing the bacterial cells to release ATP, contacting the ATP with an ATP-consuming enzyme that catalyzes a reaction, and monitoring the enzyme-catalyzed reaction. Typically, the enzyme is luciferin, and the reaction is monitored by detecting light produced by the luciferin. Other methods of the invention involve contacting a fluid sample with a support surface that binds bacterial cells, lysing the bacterial cells to release ATP, contacting the ATP with an ATP-consuming enzyme, and monitoring the enzyme-catalyzed reaction.
    Type: Application
    Filed: August 11, 2012
    Publication date: April 4, 2013
    Applicant: Zybac, LLC
    Inventors: Daniel G. Ericson, Kyle R. Brandy
  • Publication number: 20130059328
    Abstract: Disclosed is the construction of a system capable of quickly and easily identifying patients having a risk for worsening inflammatory digestive tract disease by identifying a causal factor for the worsening of the inflammatory digestive tract disease. Specifically disclosed is a method for detecting oral bacteria that cause worsening of inflammatory digestive tract disease and/or a screening that targets high risk for worsening of inflammatory digestive tract disease and/or determining the risk for worsening of the inflammatory digestive tract disease by PA not being detected and/or CBP being detected, inclusive of the detection of PA, which is an oral bacteria protein antigen, and/or CBP, which is collagen binding protein, in samples. Also disclosed are a detection reagent and kit used for this method.
    Type: Application
    Filed: March 10, 2011
    Publication date: March 7, 2013
    Applicant: Osaka University
    Inventors: Kazuo Umemura, Kazuya Hokamura, Kazuhiko Nakano, Takashi Ooshima, Ryota Nomura, Koichiro Wada
  • Patent number: 8389233
    Abstract: Provided are methods for sampling, testing and validating test lots, comprising: assembling a plurality of product portions from each of a plurality of test lots and combining the portions to provide a corresponding set of test lot samples; enriching the test lot samples; removing portions of each enriched sample, and combining the removed portions to provide a modular composite sample; and testing of the modular composite sample, and individual testing of the enriched test lot samples, using at least one suitable detection assay for a target microbe or organism, wherein when such testing is negative all test lots are validated, and wherein when such testing is positive with respect to the modular composite sample, or with respect to an individual enriched test lot sample, individual test lots may nonetheless yet be validated by further testing of a portion of respective initially-negative enriched test lot samples and obtaining negative results.
    Type: Grant
    Filed: January 27, 2009
    Date of Patent: March 5, 2013
    Assignee: Institute for Environmental Health, Inc.
    Inventor: Mansour Samadpour
  • Publication number: 20130040290
    Abstract: A monitoring assembly (201) with an intake (213) has at least one pump (210) featuring at least one pump channel mounted in the monitoring assembly (201). A plurality of fluid lines are coupled to the at least one pump (210). At least one filter cartridge (315) is also mounted in the assembly. Each filter cartridge (315) is separately coupled by one of the plurality of fluid lines to one of the pump channels, where each filter cartridge (315) contains material for extracting an analyte, and where the at least one pump operates to separately push fluid through the at least one filter cartridge (315). The filter cartridge (315) operates to separate fluid into constituent parts.
    Type: Application
    Filed: May 4, 2011
    Publication date: February 14, 2013
    Applicant: Arizona Board of Regents Acting for and on behalf of Arizona State University
    Inventor: Rolf U. Halden
  • Publication number: 20120322098
    Abstract: The invention relates to culture medium for screening or enrichment of methicillin-resistant Staphylococcus aureus (MRSA), which medium comprises a combination of at least two cephalosporins.
    Type: Application
    Filed: December 29, 2010
    Publication date: December 20, 2012
    Applicant: BIO-RAD INNOVATIONS
    Inventors: Christine Favier, Agnès Kammoun
  • Publication number: 20120270249
    Abstract: A method for distinguishing among a first group of microorganisms, belonging to a first taxon of bacteria that is vancomycin resistant; a second group of microorganisms, belonging to a second taxon of vancomycin resistant bacteria that is different than said first taxon; and a third group of microorganisms that is not resistant or has a natural resistance to vancomycin.
    Type: Application
    Filed: June 29, 2012
    Publication date: October 25, 2012
    Applicant: BIOMERIEUX
    Inventors: Sylvain ORENGA, Céline ROGER-DALBERT, John PERRY, Vanessa CHANTEPERDRIX, Gilles ZAMBARDI, Nathalie BAL
  • Patent number: 8252550
    Abstract: Immunogenic compositions and methods for eliciting an immune response against S. epidermidis and other related staphylococci are provided. The immunogenic compositions can include immunogenic conjugates of poly-?-glutamic acid (such as ?DLPGA) polypeptides of S. epidermidis, or related staphylococci that express a ?PGA polypeptide. The ?PGA conjugates elicit an effective immune response against S. epidermidis, or other staphylococci, in subjects to which the conjugates are administered. A method of treating an infection caused by a Staphylococcus organism that expresses cap genes is also disclosed. The method can include selecting a subject who is at risk of or has been diagnosed with the infection by the Staphylococcus organism which expresses ?PGA from the cap genes. Further, the expression of a ?PGA polypeptide by the organism can then be altered.
    Type: Grant
    Filed: February 25, 2011
    Date of Patent: August 28, 2012
    Assignee: The United States of America as represented by the Secretary of the Department of Health and Human Services
    Inventors: Michael Otto, Stanislava Kocianova, Cuong Vuong, Jovanka Voyich, Yufeng Yao, Elizabeth Fischer, Frank De Leo
  • Publication number: 20120171238
    Abstract: The invention discloses the use of a protein with an amino acid sequence according to SEQ. ID. NO. 1 or a naturally occurring fragment or variant thereof for identifying infections with S. aureus in a human sample. More specifically, IgE molecules specific for SEQ. ID. NO. 1 are detected in the sample of the patient. Many atopic dermatitis patients have IgE specific for SEQ. ID. NO. 1.
    Type: Application
    Filed: September 10, 2010
    Publication date: July 5, 2012
    Applicant: BIOMAY AG
    Inventors: Rudolf Valenta, Kavita Reginald, Kerstin Westritschnig, Birgit Linhart, Angelika Stöcklinger, Josef Thalhamer, Alexander M. Hirschi, Thomas Werfel, Elli Greisenegger, Tamara Kopp
  • Publication number: 20120028251
    Abstract: The disclosure provides articles and methods useful for detecting a discrete source of DNase activity. DNase-producing microorganisms can be detected. The device can further include selective agents and/or indicators to differentiate groups or species microorganisms. Methods of use include detecting or enumerating DNase-producing microorganisms.
    Type: Application
    Filed: February 22, 2010
    Publication date: February 2, 2012
    Inventors: Patrick A. Mach, Michelle L. Rosauer, Michael E. Hughes
  • Publication number: 20120015398
    Abstract: The present invention relates to a method for the semi-quantitative determination of cariogenic bacteria in a sample of saliva and/or plaque, in which the microorganisms contained in a sample of plaque or saliva are brought into contact with a carbon source that is fermented to acid by the cariogenic bacteria. The microorganisms are then incubated under conditions which make possible selective acid formation by the cariogenic bacteria. The acid formation is then detected by determining the pH at least once within a period of 12 hours after adding the carbon source, wherein the cariogenic bacteria are determined semi-quantitatively in the sample by comparison of the pH with at least one reference value. In addition, the invention provides kits for carrying out such methods.
    Type: Application
    Filed: July 12, 2011
    Publication date: January 19, 2012
    Applicant: IVOCLAR VIVADENT AG
    Inventors: Manuela Bischof, Carlo Bolis, Urs Lendenmann, Volker Rheinberger
  • Patent number: 8097404
    Abstract: The present invention relates to a composition for the permeabilization of the walls of microorganisms comprising the combination of octyl-?-D-glucopyranoside (NOG), sodium polyphosphates (HMP), and a salt chosen from lithium chloride or rubidium chloride, and a method for the enumeration and identification of cells on a membrane using said composition.
    Type: Grant
    Filed: March 20, 2009
    Date of Patent: January 17, 2012
    Assignee: Millipore Corporation
    Inventors: Sebastien Ribault, Frederic Olivieri, Herve Meder, David Lehmann, Sandra Laborde
  • Publication number: 20110300552
    Abstract: The present invention is based on the discovery that drug resistance in microorganisms can be rapidly and accurately determined using mass spectrometry. A mass spectrum of an intact microorganism or one or more isolated biomarkers from the microorganism grown in drug containing, isotopically-labeled media is compared with a mass spectrum of the intact microorganism or one or more isolated biomarkers from the microorganism grown in non-labeled media without the drug present. Drug resistance is determined by predicting and detecting a characteristic mass shift of one or more biomarkers using algorithms. The characteristic mass shift is indicative that the microorganism is growing in the presence of the drug and incorporating the isotopic label into the one or more biomarkers, resulting in change in mass.
    Type: Application
    Filed: February 17, 2011
    Publication date: December 8, 2011
    Inventors: Plamen A. Demirev, Nathan A. Hagan, Miquel D. Antoine, Jeffrey S. Lin, Andrew B. Feldman
  • Publication number: 20110275114
    Abstract: A dry sample analyzing mixture, a liquid sample analyzing medium, and a sample analyzing method, are described for use in detecting the presence or absence of Methicillin Resistant Staphylococcus Aureus (“MRSA”) in an incubated first generation biological or environmental specimen sample. First generation specimen samples that can be analyzed include nasal swabs, lesion swabs, skin swabs, throat swabs, food swabs, tanning salon swabs, gym swabs, restaurant swabs, and the like. The medium and method include an anti-ribosomal antibiotic component that will selectively prevent Methicillin Susceptible Staphylococcus Aureus (“MSSA”) from growing in the medium, while allowing MRSA to grow in the medium. The medium also includes components which will stimulate growth of MRSA. The medium also includes components which will produce a detectable signal, which signal indicates the presence of MRSA in the incubated sample.
    Type: Application
    Filed: May 6, 2010
    Publication date: November 10, 2011
    Inventor: Stephen C. Edberg
  • Publication number: 20110217312
    Abstract: Immunogenic compositions and methods for eliciting an immune response against S. epidermidis and other related staphylococci are provided. The immunogenic compositions can include immunogenic conjugates of poly-?-glutamic acid (such as ?DLPGA) polypeptides of S. epidermidis, or related staphylococci that express a ?PGA polypeptide. The ?PGA conjugates elicit an effective immune response against S. epidermidis, or other staphylococci, in subjects to which the conjugates are administered. A method of treating an infection caused by a Staphylococcus organism that expresses cap genes is also disclosed. The method can include selecting a subject who is at risk of or has been diagnosed with the infection by the Staphylococcus organism which expresses ?PGA from the cap genes. Further, the expression of a ?PGA polypeptide by the organism can then be altered.
    Type: Application
    Filed: February 25, 2011
    Publication date: September 8, 2011
    Inventors: Michael Otto, Stanislava Kocianova, Cuong Vuong, Jovanka Voyich, Yufeng Yao, Elizabeth Fischer, Frank De Leo
  • Publication number: 20110171683
    Abstract: A thin film culture device for detection of antibiotic-resistant microorganisms is provided. The device can include indicators to differentiate staphylococcal from non-staphylococcal microorganisms. Methods of use include detecting or enumerating antibiotic-resistant microorganisms. The methods further include obtaining a differential count of staphylococcal and non-staphylococcal microorganisms.
    Type: Application
    Filed: August 19, 2009
    Publication date: July 14, 2011
    Inventors: Patrick A. Mach, Michelle L. Rosauer
  • Publication number: 20110165604
    Abstract: A reaction medium for detecting and/or identifying Methicillin-resistant Staphylococcus aureus (MRSA) bacteria, comprising a predetermined combination of two antibiotics, a first antibiotic which belongs to the cephalosporin family and a second antibiotic, said first and second antibiotics each being at a sub-inhibitory concentration.
    Type: Application
    Filed: October 7, 2009
    Publication date: July 7, 2011
    Applicant: BIOMERIEUX
    Inventors: Sylvain Orenga, Denis Robichon, Gilles Zambardi