Determining Presence Or Kind Of Micro-organism; Use Of Selective Media Patents (Class 435/34)
  • Patent number: 10415075
    Abstract: A method is described to separate, characterize and/or identify microorganisms in a test sample. Said method comprises the steps of (i) lysing non-microorganism cells that might be present in the sample, (ii) separating the microorganisms as a pellet in the sample post-lysing non-microorganism cells, (iii) interrogating the pellet containing microorganisms by mass spectrometry to produce mass spectra of microorganisms, and (iv) characterizing and/or identifying microorganisms in the sample. The method may be used in characterizing/identifying microorganisms in complex samples, e.g., blood containing culture media.
    Type: Grant
    Filed: October 30, 2009
    Date of Patent: September 17, 2019
    Assignee: bioMerieux, Inc.
    Inventors: Jones Hyman, Bradford Clay, John Walsh, Thurman Thorpe
  • Patent number: 10413592
    Abstract: The present invention relates to improved assisted reproductive technology using highly purified menotropin (HP-hMG) to stimulate follicle development in controlled ovarian stimulation, particularly in women at risk of a high ovarian response to controlled ovarian stimulation.
    Type: Grant
    Filed: February 25, 2016
    Date of Patent: September 17, 2019
    Assignee: FERRING B.V.
    Inventors: Joan-Carles Arce, Jane Ruman
  • Patent number: 10415081
    Abstract: The invention provides methods and processes for the identification of polymorphisms at one ore more designated sites, without interference from non-designated sites located within proximity of such designated sites. Probes are provided capable of interrogation of such designated sites in order to determine the composition of each such designated site. By the methods of this invention, one ore more mutations within the CFTR gene and the HLA gene complex can be identified.
    Type: Grant
    Filed: August 4, 2016
    Date of Patent: September 17, 2019
    Assignee: BioArray Solutions Ltd.
    Inventors: Alice Xiang Li, Ghazala Hashmi, Michael Seul
  • Patent number: 10408735
    Abstract: An in vitro method for the marker-free determination of a cell type of a cell in a biological sample is disclosed, a microscopic device being configured to detect a height profile of the cell with respect to a carrier plate. An embodiment of the method performed by a cell analysis device, include: determining a cell compartment of the cell on the basis of the detected height profile, determining a predetermined quantitative cell feature on the basis of the determined cell compartment, and determining the cell type of the cell on the basis of the determined quantitative cell feature. A correspondingly designed cell analysis device and a corresponding microscopic device are also disclosed.
    Type: Grant
    Filed: September 30, 2014
    Date of Patent: September 10, 2019
    Assignee: Siemens Healthcare GmbH
    Inventors: Oliver Hayden, Oliver Schmidt
  • Patent number: 10406227
    Abstract: Methods and compositions modulate the activity of electrically excitable cells. Photovoltaic compounds which, upon exposure to light energy, increase or decrease the electrical activity of cells. These supplement and/or replace of vision based on the conversion of light energy to electrical energy within certain cells of the visual system. A “patch” or bridge to circumvent one or more defective, damaged, or diseased cells in the visual system. Additionally, in several embodiments, subjects with normal vision can benefit from the methods, compositions, systems, and/or devices disclosed herein as normal visual acuity can be heightened. The exposure induces an energy (e.g., a receipt of light energy, conversion to electrical energy, and passage of that electrical energy) from the photovoltaic compound to the cell, thereby altering the transmembrane potential of the cell and/or the opening of one or more ion channels, thereby modulating the activity of the electrically excitable cell.
    Type: Grant
    Filed: December 18, 2013
    Date of Patent: September 10, 2019
    Assignees: California Institute of Technology, University of Southern California
    Inventors: Robert H. Chow, Mark S. Humayun, Harry B. Gray, Robert H. Grubbs, Dennis A. Dougherty, Mark E. Thompson, Lionel E. Cheruzel, Melanie A. Yen
  • Patent number: 10392648
    Abstract: Provided are an airborne microbial measurement apparatus and a method of measuring the same. The airborne microbial measurement apparatus includes a particle separation device including a main body having a flow space in which airborne microorganism flows and a collection unit separably coupled to one side of the main body to collect the airborne microorganism, a reagent container in which a lysis reagent reacting with the airborne microorganism collected in the collection unit and a luminous material are stored, and a luminescence measurement device for measuring intensity of light emitted after the airborne microorganism reacts with the lysis reagent and the luminous material.
    Type: Grant
    Filed: December 16, 2014
    Date of Patent: August 27, 2019
    Assignees: LG ELECTRONICS INC., INDUSTRY-ACADEMIC COOPERATION FOUNDATION, YONSEI UNIVERSITY
    Inventors: Chulwoo Park, Yeekyeong Jung, Sunghwa Lee, Jungho Hwang, Jiwoon Park
  • Patent number: 10386376
    Abstract: A test device for detection and visual identification of a specific analyte in a liquid sample such as a bodily fluid is disclosed. The device includes a collection container, a test strip affixed to an interior surface of the collection container, and a removable protective strip adhered over the test strip. The protective strip is configured to be removed from contact with the test strip after the liquid sample has been collected in order to prevent unnecessary exposure and/or contamination of the test strip. In certain embodiments, the protective strip may be removed from the contact with the test strip when the collection container is in a sealed configuration. In other embodiments, the protective strip may be dissolvable when placed in contact with the desired liquid sample. An assay specific to the anticipated analyte may be provided on the test strip.
    Type: Grant
    Filed: November 9, 2015
    Date of Patent: August 20, 2019
    Assignee: JEIMEI, LLC
    Inventor: Christina Holloway
  • Patent number: 10380807
    Abstract: A pipette check station for checking the calibration or service status of a pipette includes an RFID reader, a user interface with a display and buttons, and a digital interface to connect the check station to additional equipment; the check station optionally further includes provisions to hold pipettes for storage and to charge electronic pipettes held thereupon.
    Type: Grant
    Filed: January 1, 2018
    Date of Patent: August 13, 2019
    Assignee: Mettler-Toledo Rainin, LLC
    Inventors: Gerhard Keller, Richard Hill, Deryl Stanley, James Petrek, Murray Anderson
  • Patent number: 10351896
    Abstract: Use of at least one chromogenic and/or fluorogenic carboxylesterase and/or triacylglycerol-lipase substrate, to detect bacteria of the Bacillus cereus group in a sample capable of containing them.
    Type: Grant
    Filed: December 23, 2014
    Date of Patent: July 16, 2019
    Assignee: BIOMERIEUX
    Inventors: Marie Cellier, Marie-Pierre Bourguignon, Diane Halimi
  • Patent number: 10352934
    Abstract: A test substrate for detecting a LAL-reactive substance, wherein at least a portion of said test substrate has been preloaded with at least one LAL reagent and/or at least one LAL-reactive standard. Methods of use of the test substrate are disclosed. Methods of depositing test reagents on a test substrate are also disclosed.
    Type: Grant
    Filed: September 28, 2017
    Date of Patent: July 16, 2019
    Assignee: GENERAL ELECTRIC COMPANY
    Inventors: Richard Douglas Godec, Paul Charles Melanson, Matthew Kaddeland Stonesmith, Hong Xu, Yan Huang
  • Patent number: 10345259
    Abstract: The present invention provides a method for the quantification of virus particles in a biological sample, comprising the steps of: (a) introducing said biological sample comprising virus particles into a capillary tube containing a buffer solution; (b) applying an electrical field to said capillary tube of sufficient voltage to allow for the separation of the virus particles from additional constituents in said sample, to obtain electrophoretical fractions; (c) generating an electropherogram associated with the electrophoretical fractions; and (d) determining the concentration of virus particles in said sample by comparing the electropherogram with an electropherogram generated from a reference sample containing a known concentration of said virus particles.
    Type: Grant
    Filed: February 18, 2016
    Date of Patent: July 9, 2019
    Assignee: Janssen Vaccines & Prevention B.V.
    Inventors: Ewoud Van Tricht, Lars Johannes Geurink, Catharina Elise Sänger-Van De Griend
  • Patent number: 10330584
    Abstract: A cell analyzer and a particle sorting method and device are disclosed. The method comprises: acquiring a pulse width of at least one optical signal according to a detected optical signal, selecting at least one optical signal as a combined optical signal, and respectively calculating a signal intensity of the combined optical signal with the pulse width in a combinatorial way to obtain at least one reinforcement signal, where a difference between a first category of particles and a second categories of particles in the reinforcement signal is increased relative to a difference therebetween in the combined optical signal; and on the basis of the reinforcement signal and at least another signal, forming a new scatter diagram, where the at least another signal is one of other reinforcement signals and the optical signal, distinguishing the first category of particles from the second category of particles according to the new scatter diagram.
    Type: Grant
    Filed: August 11, 2017
    Date of Patent: June 25, 2019
    Assignee: Shenzhen Mindray Bio-Medical Electronics Co., Ltd.
    Inventors: Bo Ye, Guanzhen Wang, Jiantao Di, Ying Zhang, Huan Qi
  • Patent number: 10311573
    Abstract: Systems, methods, and devices for classifying or detecting mold samples or training computer models (such as neural networks), are disclosed. A method includes obtaining a microscopy image of a mold sample. The method includes determining a classification of the mold sample based on non-image data corresponding to the mold sample. The method further includes training a computer model based on the microscopy image and a label indicating the classification.
    Type: Grant
    Filed: May 2, 2017
    Date of Patent: June 4, 2019
    Assignee: Techcyte, Inc.
    Inventors: S. Russell Zimmerman, Ralph Yarro, III, Benjamin P. Cahoon, Richard Boyd Smith, Hyrum S. Anderson
  • Patent number: 10288569
    Abstract: A method for preparing a hydrogel that reversibly changes color depending on pH includes: (S10) dissolving a carboxyl group-containing polysaccharide in buffer to obtain a polysaccharide solution; (S20) adding a cross-linker solution composed of a mixture of an organic solvent and a cross-linker to the polysaccharide solution; (S30) adding, to the cross-linker-containing polysaccharide solution resulting from step (S20), an organic solution composed of a mixture of an organic solvent and a first organic compound containing an aromatic functional group having at least one hydroxyl group bonded thereto, to form a mixture solution, and allowing the mixture solution to react; (S40) obtaining a polymer complex from the reaction mixture resulting from step (S30); and (S50) mixing the polymer complex from step (S40) with an organic dye comprising a second organic compound containing an aromatic functional group having at least one hydroxyl group bonded thereto.
    Type: Grant
    Filed: February 8, 2017
    Date of Patent: May 14, 2019
    Assignee: INDUSTRY-ACADEMIC CORPORATION FOUNDATION YONSEI UNIVERSITY
    Inventors: Sang Yup Lee, Chaemyeong Lee, Min Chul Kim, Jin Young Kwak, Sang Woo Park, Young Jun Lim
  • Patent number: 10280446
    Abstract: The disclosure provides articles and methods useful for detecting a discrete source of DNase activity. DNase-producing microorganisms can be detected. The device can further include selective agents and/or indicators to differentiate groups or species microorganisms. Methods of use include detecting or enumerating DNase-producing microorganisms.
    Type: Grant
    Filed: July 17, 2017
    Date of Patent: May 7, 2019
    Assignee: 3M Innovative Properties Company
    Inventors: Patrick A. Mach, Michelle L. Rosauer, Michael E. Hughes
  • Patent number: 10260082
    Abstract: Liposomal detection devices and methods of making and using such devices are disclosed. Such liposomal detection devices may be used to detect microbes by detecting a byproduct of microbial metabolism, or may be used to detect changes in pH and/or changes in temperature. Liposomal detection devices may include a first liposome encapsulating a first destabilizing compound and a second liposome encapsulating a second destabilizing compound. The first liposome may destabilize in response to the detection of the target compound or change to release the first destabilizing compound which may destabilize the second liposome. A matrix, such as a hydrogel matrix, may encase the first liposome and the second liposome.
    Type: Grant
    Filed: October 22, 2013
    Date of Patent: April 16, 2019
    Assignee: EMPIRE TECHNOLOGY DEVELOPMENT LLC
    Inventors: Michael Keoni Manion, George Charles Peppou
  • Patent number: 10233476
    Abstract: An apparatus (1) for analyzing a contaminated surface has a transfer device (2) with a mount (3) and a porous disk-shaped medium (5) having a contact side (6) arrangeable on the contaminated surface. The mount (3) is bindable to the medium on its side (8) facing away from the contact side (6) via a first fixing edge (7a). An analytical device (11) is bindable to the medium (5) on its contact side (6) via a second fixing edge (7b) to remove the medium (5) from the mount (3). The first fixing edge (7a) is bindable to the medium (5) via a first adhesive bond and the second fixing edge (7b) is bindable to the medium (5) via a second adhesive bond. The first adhesive bond is breakable by a lower application of force than the second adhesive bond.
    Type: Grant
    Filed: June 8, 2011
    Date of Patent: March 19, 2019
    Assignee: SARTORIUS STEDIM BIOTECH GMBH
    Inventor: Karl Pflanz
  • Patent number: 10228312
    Abstract: The present invention relates to the chromatographic isolation of a target cell or another complex biological material, in particular by column chromatography such as affinity chromatography or gel permeation chromatography. The invention employs a receptor binding reagent that binds to a receptor molecule that is located on the surface of a target cell. The invention in general provides novel methods for the traceless isolation of biologic materials such as cells, cell organelles, viruses and the like. The invention also relates to an apparatus for the isolation of cells and other complex biological materials.
    Type: Grant
    Filed: February 25, 2013
    Date of Patent: March 12, 2019
    Assignee: Juno Therapeutics GmbH
    Inventor: Herbert Stadler
  • Patent number: 10227629
    Abstract: Methods for diagnosing, treating, and monitoring the treatment of invasive aspergillosis (IA) are described. The methods can include detecting the presence of one or more volatile organic compounds (VOCs) in the breath of subjects suspected of having IA.
    Type: Grant
    Filed: June 4, 2015
    Date of Patent: March 12, 2019
    Assignee: The Brigham and Women's Hospital, Inc.
    Inventors: Sophia Koo, Horatio R. Thomas, Lindsey R. Baden, Francisco M. Marty
  • Patent number: 10175238
    Abstract: The present invention relates to methods of determining the presence or absence of abnormal lymphoid cells or abnormal myeloid cells in a cell sample, with the methods comprising subjecting the cell sample to conditions that will activate any inactive matriptase present in the cell sample and measuring the levels of activated matriptase in the cell sample. Once measured, these levels can then be compared to control levels of active matriptase to determine if the cell sample has elevated levels of activated matriptase over control levels of active matriptase. An elevation in the levels of activated matriptase in the cell sample is indicative that the cell sample contains abnormal lymphoid cells or abnormal myeloid cells.
    Type: Grant
    Filed: May 13, 2015
    Date of Patent: January 8, 2019
    Assignee: Georgetown University
    Inventors: Chen-Yong Lin, Michael D. Johnson, Feng-Pai Chou
  • Patent number: 10167494
    Abstract: The present invention provides a method and system for monitoring, detecting, and/or characterizing a biological particle that may be present in a sample. The method may be accomplished in a sealed container by utilizing a first step time-dependent spectroscopic technique to obtain at least two measurements of a growth composition comprising a sample and correlating said measurements for the detection and/or characterization of a biological particle that may be present in the sample. The method further provides for a subsequent step for the separation, characterizion and/or identification of the microorganisms in the sealed container.
    Type: Grant
    Filed: October 30, 2009
    Date of Patent: January 1, 2019
    Assignee: bioMerieux, Inc.
    Inventors: John Walsh, Jones M. Hyman, Thurman Thorpe, Bradford Clay
  • Patent number: 10157734
    Abstract: Methods and systems for identification of microorganisms either after isolation from a culture or directly from a sample. The methods and systems are configured to identify microorganisms based on the characterization of proteins of the microorganisms via high-resolution/mass accuracy single-stage (MS) or multi-stage (MSn) mass spectrometry. Included herein are also discussion of targeted detection and evaluation of virulence factors, antibiotic resistance markers, antibiotic susceptibility markers, typing, or other characteristics using a method applicable to substantially all microorganisms and high-resolution/mass accuracy single-stage (MS) or multi-stage (MSn) mass spectrometry.
    Type: Grant
    Filed: May 22, 2015
    Date of Patent: December 18, 2018
    Assignee: OXIOD LIMITED
    Inventors: James L. Stephenson, Jr., Oksana Gvozdyak, Roger Grist, Clay Campbell, Iain C. Mylchreest, Ian D. Jardine
  • Patent number: 10137423
    Abstract: Disclosed herein are devices and methods for the detection, quantification and/or monitoring of analytes. The systems and methods can be used, for example, to rapidly monitor gases downhole in a well.
    Type: Grant
    Filed: July 27, 2015
    Date of Patent: November 27, 2018
    Assignee: SCHLUMBERGER TECHNOLOGY CORPORATION
    Inventors: Kay McGuinness, Nathan Scott Lawrence, Andrew Meredith, Andrew Warrillow, Laurent Pirolli
  • Patent number: 10130665
    Abstract: A method, for the identification of bacterial isolates suitable for use in bacteriotherapy, the method comprising: (i) preparing a suspension of material collected from a host harboring microbiota; (ii) addition of an activator of bacterial spores sufficient to allow growth of bacteria from spores present in the suspension; (iii) culturing the suspension; and (iv) identification of at least one bacterial species within the culture.
    Type: Grant
    Filed: May 20, 2013
    Date of Patent: November 20, 2018
    Assignee: GENOME RESEARCH LIMITED
    Inventors: Trevor D. Lawley, Simon Clare, Gordon Dougan
  • Patent number: 10126243
    Abstract: A plankton examination apparatus comprises: a sub-pipe in which a transparent pipe unit; a light source unit which radiates only blue-series light; a detection unit which receives light radiated in the direction on the other side outside the transparent pipe unit through the transparent pipe unit from the light source unit and generates an image of the plankton; and a dichroic mirror installed between the transparent pipe unit and the detection unit in such a way as to transmit only red-series light which belongs to the light radiated by the light source unit and passed through the transparent pipe unit and has a wavelength of 620 nm to 780 nm and to deliver the red-series light to the detection unit.
    Type: Grant
    Filed: June 25, 2014
    Date of Patent: November 13, 2018
    Assignee: KOREA OCEANIC AND ATMOSPHERIC SYSTEM TECHNOLOGY (KOAST)
    Inventors: Hyo Hyuc Im, Kyu Young Choi, Deok Whan Choi
  • Patent number: 10112976
    Abstract: The invention relates to a process for solution-phase synthesis of D-Arginyl-2,6-dimethyl-L-tyrosyl-L-lysyl-L-phenylalaninamide, an active ingredient used for both common and rare diseases including a mitochondrial targeted therapy for ischemia reperfusion injury.
    Type: Grant
    Filed: June 24, 2015
    Date of Patent: October 30, 2018
    Assignee: FLAMMA S.P.A.
    Inventors: Xinjun Zhao, Minyu Zheng, Xiaozhong Yu, Hanrong Gao, Fabrice Cornille
  • Patent number: 10107803
    Abstract: In one example, a device includes a trench formed in a substrate. The trench includes a first end and a second end that are non-collinear. A first plurality of semiconductor pillars is positioned near the first end of the trench and includes integrated light sources. A second plurality of semiconductor pillars is positioned near the second end of the trench and includes integrated photodetectors.
    Type: Grant
    Filed: August 23, 2016
    Date of Patent: October 23, 2018
    Assignee: International Business Machines Corporation
    Inventors: Yann Astier, Huan Hu, Ning Li, Devendra K. Sadana, Joshua T. Smith, William T. Spratt
  • Patent number: 10093920
    Abstract: The present invention relates to methods for screening a polypeptide for desired activity against a target molecule In particular, the present invention relates to methods for screening a polypeptide for desired activity against a target molecule by expressing the polypeptide in a bacterial cell and permeabilizing the cell.
    Type: Grant
    Filed: December 20, 2010
    Date of Patent: October 9, 2018
    Assignee: AFFINITY BIOSCIENCES PTY LTD
    Inventors: Matthew Beasley, Ben Kiefel
  • Patent number: 10077464
    Abstract: A microorganism detection medium, said detection being based on showing the presence of a microbial enzyme activity chosen from esterase and/or osidase and/or peptidase and/or sulfatase and/or phosphatase activities of microorganisms, preferably said microbial enzyme activity being an esterase activity, said medium comprising: at least one chromogenic and/or fluorogenic substrate specific to the enzyme activity sought, preferably specific to an esterase activity, at least one alkyl(thio)glycoside, at least one solvent (S).
    Type: Grant
    Filed: December 24, 2013
    Date of Patent: September 18, 2018
    Assignee: Biomerieux
    Inventors: Marie Cellier, Sylvain Orenga, John Perry
  • Patent number: 10059976
    Abstract: Provided is a microorganism detection method which is highly versatile and applicable to both the test for the detection of specified microorganisms and the microbial enumeration test under the same test conditions for Kampo extract preparations and crude drugs containing a variety of antimicrobial substances, is able to effectively reduce the influence of antimicrobial substances in a test sample, and may detect target microorganisms with high precision. This method is a microorganism detection method in a test sample having antimicrobial activity, including a step of preparing a sample liquid containing a test sample having antimicrobial activity and a step of culturing a target microorganism in a culture medium, the method including allowing a (meth)acrylic acid ester-based synthetic adsorbent to act on the sample liquid and/or the culture medium.
    Type: Grant
    Filed: June 10, 2014
    Date of Patent: August 28, 2018
    Assignee: TSUMURA & Co.
    Inventors: Hiroaki Yamamoto, Isao Fukuda
  • Patent number: 10041923
    Abstract: A system for measuring water quality parameters, such as pH, CO2, alkalinity, and so forth, includes sensors or other measuring components integrated onto a circuit board. The circuit board may be contained within a submersible, watertight housing. A spectrophotometer may be located on the circuit board to measure the wavelength and intensity of light transmitted through the water being tested. Sensors for measuring salinity, pressure, temperature, or other properties may also be included on or near the circuit board. A microcontroller for processing these measurements is located on the circuit board. System components used to direct and filter the water, such as certain pumps, filters, and so forth, may be included in the housing off of the circuit board. Integrating the sensors and other measuring components onto a circuit board allows for a compact, low-cost water-quality-measuring system that is capable of making very accurate and precise measurements.
    Type: Grant
    Filed: April 12, 2017
    Date of Patent: August 7, 2018
    Assignee: SWIFT ENGINEERING, INC.
    Inventors: Andrew Streett, Marc Rocklinger, Xuewu Liu
  • Patent number: 10017808
    Abstract: A method for analyzing planar sample is provided. In some cases the method comprises: (a) incubating the planar sample with a capture agent that is linked to an oligonucleotide, wherein the capture agent specifically binds to complementary sites in the planar sample; (b) reading a fluorescent signal caused by extension of a primer that is hybridized to the oligonucleotide, using fluorescence microscopy. Several implementations of the method, and multiplexed versions of the same, are also provided.
    Type: Grant
    Filed: December 5, 2017
    Date of Patent: July 10, 2018
    Assignee: The Board of Trustees of the Leland Stanford Junior University
    Inventors: Nikolay Samusik, Garry P. Nolan, Yury Goltsev
  • Patent number: 10006082
    Abstract: A method for analyzing planar sample is provided. In some cases the method comprises: (a) incubating the planar sample with a capture agent that is linked to an oligonucleotide, wherein the capture agent specifically binds to complementary sites in the planar sample; (b) reading a fluorescent signal caused by extension of a primer that is hybridized to the oligonucleotide, using fluorescence microscopy. Several implementations of the method, and multiplexed versions of the same, are also provided.
    Type: Grant
    Filed: December 5, 2017
    Date of Patent: June 26, 2018
    Assignee: The Board of Trustees of the Leland Stanford Junior University
    Inventors: Nikolay Samusik, Garry P. Nolan, Yury Goltsev
  • Patent number: 10000796
    Abstract: A method for analyzing planar sample is provided. In some cases the method comprises: (a) incubating the planar sample with a capture agent that is linked to an oligonucleotide, wherein the capture agent specifically binds to complementary sites in the planar sample; (b) reading a fluorescent signal caused by extension of a primer that is hybridized to the oligonucleotide, using fluorescence microscopy. Several implementations of the method, and multiplexed versions of the same, are also provided.
    Type: Grant
    Filed: December 5, 2017
    Date of Patent: June 19, 2018
    Assignee: The Board of Trustees of the Leland Stanford Junior University
    Inventors: Nikolay Samusik, Garry P. Nolan, Yury Goltsev
  • Patent number: 9995738
    Abstract: The present invention relates to an apparatus and method for detecting and enumerating a rare cell in blood. The apparatus comprises a sample collector configured to collect a blood sample and receive a complex in which a target antibody and a marker are coupled; a measuring kit connected to the sample collector in such a way that a mixture of the blood sample and the complex is injected into the measuring kit, thereby individually trapping a blood cell; and a detector configured to detect and enumerate the rare cell having an antigen-antibody reaction with the target antibody among the blood cell trapped in the measuring kit.
    Type: Grant
    Filed: March 13, 2014
    Date of Patent: June 12, 2018
    Assignee: Korea University Research and Business Foundation
    Inventors: Sehyun Shin, Chang-Soo Han, Dae Ho Jang
  • Patent number: 9976940
    Abstract: The present invention relates to a composition for DNA staining, which can be safely used for researchers, can prevent DNA photocleavage, and can be applied to various fields. According to the present invention, when DNA is stained by using a fluorescent protein and a particular peptide bound to DNA, such a fluorescent protein-peptide is safe to researchers, suppresses DNA photocleavage, and allows reversible staining, thereby enabling efficient DNA staining. Therefore, the composition for DNA staining of the present invention can be variously used in DNA-related research.
    Type: Grant
    Filed: September 9, 2015
    Date of Patent: May 22, 2018
    Assignee: INDUSTRY-UNIVERSITY COOPERATION FOUNDATION SOGANG UNIVERSITY
    Inventors: Kyu Bong Jo, Seong Hyun Lee, David C. Schwartz
  • Patent number: 9977041
    Abstract: It is determined whether an automatic analysis device is in a state where it is necessary to perform periodic cleaning or periodic replacement on a B/F separation passage of a reaction liquid suction nozzle 120 for B/F separation or the like and a detection passage of a reaction liquid suction nozzle 123 for detection, a detection unit 124, and the like, based on the properties of a specimen, a reagent, and a reaction liquid which is obtained by reacting the specimen and the reagent, an analysis protocol which defines treatment conditions of these solutions, and the number of times of dispensing, feeding, and measuring the solutions; and the determined result is displayed on a display 130 as a signal. Accordingly, the automatic analysis device is provided so as to be able to perform adequate maintenance in accordance with analysis conditions.
    Type: Grant
    Filed: December 3, 2013
    Date of Patent: May 22, 2018
    Assignee: Hitachi High-Technologies Corporation
    Inventors: Yoshihiro Yamashita, Takaaki Hagiwara, Toshiharu Suzuki
  • Patent number: 9964556
    Abstract: Microplate reader has measuring space, action source, measuring device for biological structure signals of microplate wells, transport support for positioning wells in relation to an optical axis of the device and controller for the action source, the measuring device and the transport support. An incubation apparatus with a frame accommodates a microplate with wells having bottoms for reducing liquid evaporation. The frame has a first opening surrounded by an inner wall for the microplate and an outer wall parallel to the inner wall and connected thereto by an intermediate bottom so that a channel is formed and the intermediate bottom for accommodating a liquid adjusted to the content of the microplate wells. The incubation apparatus has a support surface with a second opening for a microplate and, as a result, at least a portion of the well bottoms is freely accessible by the second opening.
    Type: Grant
    Filed: October 30, 2014
    Date of Patent: May 8, 2018
    Assignee: TECAN TRADING AG
    Inventors: Gyoergy Wenczel, Harald Gebetsroither, Friedrich Menges
  • Patent number: 9958436
    Abstract: Methods for determining cell chirality using micropatterned substrates are disclosed. Also provided are methods for diagnosing diseases such as genetic diseases or cancer by comparing the chirality of sample cells from a subject with normal cells, and determining a difference in chirality between the sample cells and normal cells.
    Type: Grant
    Filed: June 15, 2012
    Date of Patent: May 1, 2018
    Assignee: THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK
    Inventors: Gordana Vunjak-Novakovic, Leo Qun Wan
  • Patent number: 9939353
    Abstract: An apparatus for cell observation and a method for cell collection using the same are disclosed. The apparatus for cell selection comprises a first substrate having an opening; and a second substrate having a photoresist unit disposed on a surface thereof, wherein the photoresist unit comprises at least one notch and defines a space which is interconnected with the notch and corresponds to the opening of the first substrate.
    Type: Grant
    Filed: March 25, 2015
    Date of Patent: April 10, 2018
    Assignee: NATIONAL TSING HUA UNIVERSITY
    Inventors: Fan-Gang Tseng, Jui-Chia Chang, Tsung-Ju Chen
  • Patent number: 9932645
    Abstract: The present invention relates to a method for detecting bacteriolytic conditions in a test sample comprising the step of contacting the test sample with a bacterial strain and a substrate which undergoes a detectable change when cleaved by a specific enzyme of said bacterial strain, wherein the bacterial strain is unable of: (a) uptaking the substrate from the medium and (b) secreting the enzyme specific for the substrate outside of the cell and wherein detecting a change in the test sample due to the cleavage of the substrate by its specific bacterial enzyme indicates that bacteriolytic conditions exist in the test sample. The invention further provides a kit for performing the method of the invention.
    Type: Grant
    Filed: November 12, 2014
    Date of Patent: April 3, 2018
    Assignee: UNIVERSITAT DE BARCELONA
    Inventors: Maria Teresa Muniesa Pérez, Lejla Imamovic, Elisenda Ballesté Pau, Anicet Blanch Gisbert, Francisco Lucena Gutiérrez, Joan Jofre Torroella
  • Patent number: 9932620
    Abstract: A rapid, sensitive method of separating and detecting microorganisms from a sample potentially containing microorganisms, such as but not limited to bacteria, fungi, yeast, viruses, and the like. The method relies on separation techniques to separate and concentrate the cells from the sample, together with chemical techniques to amplify the amount of detectable signal from low numbers of cells to provide a rapid and sensitive method of detecting microorganisms. This detection method may utilize: a filtration device; a centrifugation device; a system; a swab device; and kit comprising one or more of the devices and components to perform the present method of separating and detecting microorganisms in a sample potentially containing microorganisms. The sample may be a chemical, cosmetic, personal care, pharmaceutical, or consumable good in its raw material, in-process, and/or finished product states that needs to be tested for any contaminating microorganisms prior to shipment to the consumer.
    Type: Grant
    Filed: June 29, 2012
    Date of Patent: April 3, 2018
    Assignee: CELSIS LTD.
    Inventors: Subramani Sellappan, Andrew Hearn, Salvatore Seminara
  • Patent number: 9933446
    Abstract: This disclosure is directed to information management techniques that may be used during automated processing of biological growth media. In one embodiment, a method comprises reading an identification element associated with a biological growth medium, identifying manufacturing information associated with the biological growth medium based on the identification element, wherein the manufacturing information includes one or more manufacturing variables measured from the biological growth medium when the biological growth medium was manufactured, adjusting one or more processing parameters associated with processing of the biological growth medium based on the manufacturing variables measured from the biological growth medium when the biological growth medium was manufactured, and processing the biological growth medium in an automated system based on the processing parameters.
    Type: Grant
    Filed: February 27, 2009
    Date of Patent: April 3, 2018
    Assignee: 3M Innovative Properties Company
    Inventors: Phillip A. Bolea, Michael E. Hughes
  • Patent number: 9915813
    Abstract: The invention relates to a system and a method for determination of a value for at least one parameter describing microbial activity of individual biological organisms in a liquid sample. Images, wherein individual biological organisms may be identified, are combined to provide optical sectionings of the biological organisms and the optical sectionings are analyzed to determine the value for said at least one parameter describing microbial activity of said individual biological organisms in each sample container.
    Type: Grant
    Filed: December 1, 2010
    Date of Patent: March 13, 2018
    Assignee: KONINKLIJKE PHILIPS N.V.
    Inventors: Tom Olesen, Martin Christian Valvik, Niels Agersnap Larsen
  • Patent number: 9908912
    Abstract: A selective agent comprising a triclosan derivative for use in selective inhibition of non-target cells in a mixed population of target and non-target cells. Preferably the triclosan derivative is a glycoside derivative, more preferably a pyranoside derivative. Suitably a selective medium comprising said selective agent and methods of culturing cells using the selective agent are provided.
    Type: Grant
    Filed: November 26, 2013
    Date of Patent: March 6, 2018
    Assignee: Oxoid Limited
    Inventors: Richard Bovill, Gemma Howse
  • Patent number: 9909990
    Abstract: Naphthalene, benzene, toluene, xylene, and other volatile organic compounds VOCs have been identified as serious health hazards. Embodiments of the invention are directed to methods and apparatus for near-real-time in-situ detection and accumulated dose measurement of exposure to naphthalene vapor and other hazardous gaseous VOCs. The methods and apparatus employ excitation of fluorophors native or endogenous to compounds of interest using light sources emitting in the ultraviolet below 300 nm and measurement of native fluorescence emissions in distinct wavebands above the excitation wavelength. The apparatus of some embodiments are cell-phone-sized sensor/dosimeter “badges” to be worn by personnel potentially exposed to hazardous VOCs. The badge sensor of some embodiments provides both real time detection and data logging of exposure to naphthalene or other VOCs of interest from which both instantaneous and accumulated dose can be determined.
    Type: Grant
    Filed: September 12, 2016
    Date of Patent: March 6, 2018
    Assignee: Photon Systems, Inc.
    Inventors: William F. Hug, Rohit Bhartia, Ray D. Reid, Arthur L. Lane
  • Patent number: 9909167
    Abstract: A method for analyzing planar sample is provided. In some cases the method comprises: (a) incubating the planar sample with a capture agent that is linked to an oligonucleotide, wherein the capture agent specifically binds to complementary sites in the planar sample; (b) reading a fluorescent signal caused by extension of a primer that is hybridized to the oligonucleotide, using fluorescence microscopy. Several implementations of the method, and multiplexed versions of the same, are also provided.
    Type: Grant
    Filed: December 4, 2014
    Date of Patent: March 6, 2018
    Assignee: The Board of Trustees of the Leland Stanford Junior University
    Inventors: Nikolay Samusik, Garry P. Nolan, Yury Goltsev
  • Patent number: 9862985
    Abstract: Devices, systems, and methods for strain-specific identification and assessment of susceptibility of microorganisms based on the response of sensors in a colorimetric sensor array to metabolic products of the microorganism.
    Type: Grant
    Filed: April 26, 2013
    Date of Patent: January 9, 2018
    Assignee: Specific Technologies LLC
    Inventors: Sung Hyun Lim, Raymond Anthony Martino, Paul A. Rhodes
  • Patent number: 9850457
    Abstract: The disclosure generally relates to a test device that detects microorganism growth by detecting a gas metabolite (e.g., carbon dioxide) produced during the growth of bacteria or other microorganism in a tested sample. The test device can contain a culture growth media separated from a detection area by a gas-permeable membrane. The gas-permeable membrane permits carbon dioxide to permeate into the detection area. The detection area includes a solidified mixture of pH indicators and a gelling agent in the form of a semi-permeable matrix. The optical properties, including the absorbance of light at various wavelengths, of the detection solution change with alterations in carbon dioxide concentration. This test device can then be placed in an incubation and optical detection instrument to monitor changes in optical properties of the detection are induced during microorganism growth in the culture medium.
    Type: Grant
    Filed: December 23, 2010
    Date of Patent: December 26, 2017
    Assignee: NEOGEN CORPORATION
    Inventors: Ronald Waldo Sarver, Jr., Alexandr Y. Kariagin, Christine Claire Cooper, Susan Teruko McDougal
  • Patent number: 9849093
    Abstract: Provided are novel methods and compositions for administration of pharmaceuticals to subjects with dysphagia. Pharmaceuticals are associated with beads and administered with food, beverage or cosmetic to provide ease of administration to subjects with dysphagia. Environmental indicators are associated with beads for Quality Assurance.
    Type: Grant
    Filed: March 14, 2014
    Date of Patent: December 26, 2017
    Assignee: Cape Spear Pharmaceuticals, Ltd.
    Inventors: David Barnes, Jonathan Barker, Stephen Haggerty