Determining Presence Or Kind Of Micro-organism; Use Of Selective Media Patents (Class 435/34)
  • Patent number: 10774362
    Abstract: Sample preparation unit, preferably for sterility testing: a housing body including at least two ports adapted to serve as fluid inlet and/or fluid outlet, a membrane support, and a lid part such that a membrane chamber is defined adjacent said membrane support. One of at least two ports is arranged so as to allow a fluid transfer to/from a first volume of the membrane chamber at a position upstream of a membrane to be placed on the membrane support, and the other ports arranged to allow fluid transfer to/from a second volume of said membrane chamber at a position downstream of a membrane to be placed on said membrane support. A movable part is provided on housing body such that the movable part and housing body are movable relative to each other, selectively interrupting/establishing fluid transfer between at least one of two ports and the membrane chamber.
    Type: Grant
    Filed: December 4, 2014
    Date of Patent: September 15, 2020
    Assignee: Merck Patent GmbH
    Inventors: Anne Baumstummler, Marie Pressel, Sandra Laborde, David Lehmann, Gael Waiche
  • Patent number: 10774039
    Abstract: The invention relates to a polypeptide comprising an amino acid having a cyclopropene group wherein said cyclopropene group is joined to the amino acid via a carbamate group. Suitably the cyclopropene group is a 1,3-disubstituted cyclopropene such as a 1,3-dimethylcyclopropene. Suitably the cyclopropene group is present as a residue of a lysine amino acid. The invention also relates to methods of making the polypeptides. The invention also relates to an amino acid comprising cyclopropene wherein said cyclopropene group is joined to the amino acid moiety via a carbamate group.
    Type: Grant
    Filed: March 10, 2015
    Date of Patent: September 15, 2020
    Assignee: United Kingdom Research and Innovation
    Inventor: Thomas Elliott
  • Patent number: 10765069
    Abstract: Embodiments include a computer-implemented method, system, and computer program product for supplementing sub-optimal environmental conditions for plant growth, the embodiments include receiving plant data for a plant, and extracting optimal growth condition data from a database based at least in part on the plant. Embodiments also include obtaining sensor data from sensors monitoring current growth conditions of the plant and comparing the current growth conditions of the plant to the optimal growth condition data from the database to estimate a current cytoplasmic streaming rate. Embodiments include responsive to the comparison, calculating, by the calculation engine, a sound wave frequency to stimulate cytoplasmic streaming in the plant, transmitting the sound wave frequency to a speaker, and providing the sound wave frequency to the plant.
    Type: Grant
    Filed: May 17, 2018
    Date of Patent: September 8, 2020
    Assignee: INTERNATIONAL BUSINESS MACHINES CORPORATION
    Inventors: John S. Werner, Anuradha Rao, Pasquale A. Catalano, Arkadiy O. Tsfasman, Andrew G. Crimmins
  • Patent number: 10732186
    Abstract: The invention involves assays, diagnostics, kits, and assay components for determining levels of glycated CD59 in the assessment of gestational diabetes mellitus and/or related disorders and/or conditions.
    Type: Grant
    Filed: December 3, 2014
    Date of Patent: August 4, 2020
    Assignee: President and Fellows of Harvard College
    Inventors: Michael Chorev, Jose Alberto Halperin
  • Patent number: 10729325
    Abstract: The disclosure herein provides methods, systems, and devices for virtually staining biological tissue for enhanced visualization without use of an actual dye or tag by detecting how each pixel of an unstained tissue image changes in waveform after staining with a certain dye(s) and/or tag(s) or other transformation under a certain electromagnetic radiation source, developing a virtual staining transform based on such detection, and applying such virtual staining transform to an unstained biological tissue to virtually stain the tissue.
    Type: Grant
    Filed: March 25, 2014
    Date of Patent: August 4, 2020
    Assignee: Genetic Innovations, Inc.
    Inventors: Peter Bryant-Greenwood, Kevin P. Rosenblatt, Jeffrey N. Yu
  • Patent number: 10724095
    Abstract: The invention provides diagnostic markers of immunosenescence and methods of identifying individuals with impaired immune function based on the expression level of a combination of such markers in a biological sample obtained from said individual. Such combination of markers is useful for determining the susceptibility to nosocomial infections of an individual. Such combination of markers is also useful for predicting whether an individual will respond to active vaccination and become protected against recurring diseases.
    Type: Grant
    Filed: December 15, 2016
    Date of Patent: July 28, 2020
    Assignees: INSERM (INSTITUT NATIONAL DE LA SANTÉ ET DE LA RECHERCHE MÉDICALE), ASSISTANCE PUBLIQUE-HÔPITAUX DE PARIS (APHP), UNIVERSITÉ PARIS-EST CRÉTEIL VAL DE MARNE
    Inventors: Sophie Hue, Yves Levy
  • Patent number: 10704079
    Abstract: Presented herein are methods for the detection of specific beta-lactamases, including class A serine carbapenemases, metallo-beta-lactamases, AmpC beta-lactamases, and extended-spectrum beta-lactamases (ESBLs). The methods presented herein include methods that permit the detection of the presence of specific beta-lactamases in bacterial samples within as few as 2 to 10 minutes.
    Type: Grant
    Filed: February 26, 2018
    Date of Patent: July 7, 2020
    Assignee: Becton, Dickinson and Company
    Inventors: Mei Yang-Woytowitz, Charles Yu, Timothy Wiles
  • Patent number: 10697871
    Abstract: The present invention encompasses a micro-fluidic system having a closed-loop configuration in which inertial micro-fluidic separation of particles and/or cells is continuously repeated by feeding part of the output back to the input so that the purity and/or concentration of the particles and/or cell is maximized. The invention also includes methods of using the micro-fluidic system.
    Type: Grant
    Filed: October 6, 2017
    Date of Patent: June 30, 2020
    Assignee: Massachusetts Institute of Technology
    Inventors: Hyunryul Ryu, Kyungyong Choi, Jongyoon Han
  • Patent number: 10657443
    Abstract: A method of training a learning machine to detect spills of hydrocarbon liquids from pipelines. A neural network is trained by collecting samples of a number of different ground materials as well as a number of liquid hydrocarbons. For each hydrocarbon, a spill is simulated on each ground material. For each of these spills, a thermal camera and a visible light camera are used to capture images. The images from the two cameras are fused, and input to the neural network for classification training. Once the neural network is trained, a system having the two cameras and the neural network can be used to detect actual hydrocarbon spills.
    Type: Grant
    Filed: May 23, 2018
    Date of Patent: May 19, 2020
    Assignee: Southwest Research Institute
    Inventors: Maria S. Araujo, Samantha G. Blaisdell, Daniel S. Davila, Edmond M. DuPont, Sue A. Baldor, Shane P. Siebenaler
  • Patent number: 10656143
    Abstract: The present disclosure relates to the field of medical technology, which provides methods and apparatuses for identifying red blood cells infected by plasmodium. The methods may include: obtaining a forward-scattered light signal, a side-scattered light signal and an optional fluorescence signal from cells in a blood sample; obtaining a first two-dimensional scattergram according to the forward-scattered light signal and the side-scattered light signal, or obtaining a three-dimensional scattergram according to the forward-scattered light signal, the side-scattered light signal and the fluorescence signal; and identifying cells located in a predetermined area of the first two-dimensional scattergram or the three-dimensional scattergram as the red blood cells infected by plasmodium. The apparatuses perform the methods. The methods and apparatuses can have better identification accuracy.
    Type: Grant
    Filed: October 31, 2012
    Date of Patent: May 19, 2020
    Assignee: SHENZHEN MINDRAY BIO-MEDICAL ELECTRONICS CO., LTD.
    Inventors: Bo Ye, Cheng Qian, Huan Qi
  • Patent number: 10640804
    Abstract: Methods of detecting biologicals in samples is provided herein. The detection is based on the formation of aggregates. The disclosed compositions include labeling particles and/or aggregating particles. The labeling particles and the aggregating particles may each include a receptor bound to the particle. The receptor can be either directly attached to the particle or indirectly attached to the particle through a linker. One method of detection may be visual and another may include advanced quantification of the formed aggregates.
    Type: Grant
    Filed: March 13, 2014
    Date of Patent: May 5, 2020
    Assignee: RAN Biotechnologies, Inc.
    Inventor: Roger A. Nassar
  • Patent number: 10626461
    Abstract: The invention relates to a sample processing apparatus comprising a holder for a microtiter plate comprising a plurality of microwells, optical measurement unit for measuring optical responses of samples dosed to the microwells, and a computing unit configured to analyze the optical responses in order to detect dosing failures in said plurality of microwells, and if a dosing failure has been detected in one or more of the microwells, to communicate the existence of the dosing failure to a user of the apparatus through signaling means or to store data indicative of the dosing failure to data storage means for further use. In particular, the invention relates to detecting dosing failures in before, during and after a PCR process.
    Type: Grant
    Filed: May 25, 2018
    Date of Patent: April 21, 2020
    Assignee: Thermo Fisher Scientific Baltics UAB
    Inventors: Jukka Tuunanen, Jaako Kurkela, Katja Eklin
  • Patent number: 10631031
    Abstract: A system and method are provided for collection and of a biologic sample. The method comprises collecting by a user of a testing device a biologic sample for use with the testing device, assigning correlative values as test results, and receiving the test results at a server disposed on a network. Some aspects also include presenting audiovisual messages to the user while the user is waiting for test results to be completed. These audiovisual messages are presented to the user by a mobile application. The audiovisual messages may take several forms, including advertisements and television channels.
    Type: Grant
    Filed: December 14, 2017
    Date of Patent: April 21, 2020
    Assignee: RELIANT IMMUNE DIAGNOSTICS, INC.
    Inventors: Jovan Hutton Pulitzer, Henry Joseph Legere, III
  • Patent number: 10627332
    Abstract: A cell analyzer and a sorting method for the cell analyzer are disclosed. Multiple optical signals generated by each of particles irradiated with light in a blood sample in a detection region are collected. The particles includes a first category of particles and a second category of particles. For each of the particles, Intensities of a first group of optical signals, which includes at least two optical signals selected from the multiple optical signals, and a pulse width of a second group of optical signals, which includes at least one optical signal selected from the multiple optical signals are acquired. For each of the particles, one or more reinforcement signals related to the particle are calculated based on an intensity of a first optical signal selected from the first group of optical signals and a pulse width of a second optical signal selected from the second group of optical signals, where the first optical signal is as same as or different from the second optical signal.
    Type: Grant
    Filed: May 16, 2019
    Date of Patent: April 21, 2020
    Assignee: Shenzhen Mindray Bio-Medical Electronics Co., Ltd.
    Inventors: Bo Ye, Guanzhen Wang, Jiantao Di, Ying Zhang, Huan Qi
  • Patent number: 10614287
    Abstract: A cell visualization system includes a digital holographic microscopy (DHM) device, a training device, and a virtual staining device. The DHM device produces DHM images of cells and the virtual staining device colorizes the DHM images based on an algorithm generated by the training device using generative adversarial networks and unpaired training data. A computer-implemented method for producing a virtually stained DHM image includes acquiring an image conversion algorithm which was trained using the generative adversarial networks, receiving a DHM image with depictions of one or more cells and virtually staining the DHM image by processing the DHM image using the image conversion algorithm. The virtually stained DHM image includes digital colorization of the one or more cells to imitate the appearance of a corresponding actually stained cell.
    Type: Grant
    Filed: April 24, 2018
    Date of Patent: April 7, 2020
    Assignee: Siemens Healthcare Diagnostics Inc.
    Inventors: Noha Youssry El-Zehiry, Saikiran Rapaka, Ali Kamen
  • Patent number: 10597692
    Abstract: The invention relates to methods and instruments for the rapid detection and rapid mass spectrometric identification of microbial infective agents in blood or other body fluids. The invention recognizes that blood is not a good environment for the cultivation of microbes and provides a method which (a) largely destroys or dissolves the human particles in body fluids, such as erythrocytes and leukocytes in blood, without impairing the ability of the microbes to reproduce, (b) separates the microbial pathogens from the fluid, (c) cultivates them in a nutrient broth which contains none of the antimicrobial components of the body fluids, (d) separates them from the nutrient broth, and (e) identifies the microbes by a mass spectrum of the microbial proteins. The dissolution of the human particles also releases the microbes nesting in macrophages.
    Type: Grant
    Filed: May 18, 2018
    Date of Patent: March 24, 2020
    Assignee: Bruker Daltonik GmbH
    Inventors: Jochen Franzen, Markus Kostrzewa, Thomas Maier, Karsten Michelmann, Wolfgang Pusch
  • Patent number: 10585088
    Abstract: Processes and compositions are described for preparing new, colloidally stable, coated nanomagnetic particles useful for both in-vitro and in-vivo biomedical applications, including cell targeting and capturing cells, microorganisms, and cellular organelles or entities such as exosomes. These nanomagnetic particles can also be used as imaging contrast agents due to their small size and high magnetic moment. The nanomagnetic particles include a series of sequentially added, stabilizing surface coatings rendered onto nano-sized magnetic crystal clusters (e.g., magnetite particles) to impart colloidal stability in complex biological samples with minimal leaching of the coating materials, high binding capacity, and low non-specific binding. Another benefit of this invention is the ability to utilize both external and internal magnetic field-generating separation devices to effect separation of the magnetic nanoparticles.
    Type: Grant
    Filed: April 30, 2016
    Date of Patent: March 10, 2020
    Assignee: BioLegend, Inc.
    Inventors: Dhanesh Gohel, Hong Zhang, John Ransom
  • Patent number: 10557162
    Abstract: Rapid methods that identify sepsis-causing bacteria or yeast aid the physician in critical therapeutic decision-making, thus decreasing patient mortality rates. The methods described herein employ plating microorganisms directly on to a MALDI-MS plate, adding concentrated formic acid, and identifying the microorganism by mass spectrometry. Optionally, an organic solvent may be combined with the formic acid, or added to the sample before or after the concentrated formic acid is added thereto. The methods enable direct extraction of proteins from microorganisms without the need for liquid protein extraction methods and yields positive identification results for gram-positive bacteria, gram-negative bacteria and yeast in minutes.
    Type: Grant
    Filed: March 29, 2017
    Date of Patent: February 11, 2020
    Assignee: BECTON, DICKINSON AND COMPANY
    Inventors: Liping Feng, William B. Brasso, Susan M. Kircher, Vanda White, Song Shi, Xiao Mo, Tuan-Linh Ngoc Nguyen, Adrien P. Malick, Jon E. Salomon, John D. Mantlo
  • Patent number: 10545138
    Abstract: Processes and compositions are described for preparing new, colloidally stable, coated nanomagnetic particles useful for both in-vitro and in-vivo biomedical applications, including cell targeting and capturing cells, microorganisms, and cellular organelles or entities such as exosomes. These nanomagnetic particles can also be used as imaging contrast agents due to their small size and high magnetic moment. The nanomagnetic particles include a series of sequentially added, stabilizing surface coatings rendered onto nano-sized magnetic crystal clusters (e.g., magnetite particles) to impart colloidal stability in complex biological samples with minimal leaching of the coating materials, high binding capacity, and low non-specific binding. Another benefit of this invention is the ability to utilize both external and internal magnetic field-generating separation devices to effect separation of the magnetic nanoparticles.
    Type: Grant
    Filed: April 30, 2016
    Date of Patent: January 28, 2020
    Assignee: BioLegend, Inc.
    Inventors: Dhanesh Gohel, Hong Zhang, John Ransom
  • Patent number: 10539569
    Abstract: An apparatus for processing time-of-flight mass spectrometry (TOF MS) data is disclosed. The apparatus may determine at least one candidate microorganism group based on a first molecular weight range in TOF MS data of a sample, determine a second molecular weight range based on a characteristic of the candidate microorganism group in response to a plurality of candidate microorganisms being included in the candidate microorganism group, and identify a microorganism included in the sample from among the candidate microorganisms based on the second molecular weight range in the TOF MS data.
    Type: Grant
    Filed: January 19, 2018
    Date of Patent: January 21, 2020
    Assignee: ASTA Corporation
    Inventor: Yangsun Kim
  • Patent number: 10532085
    Abstract: The present invention relates to a bioactive agent capable of increasing the intracellular concentration and/or activity of Hsp70 for use in the treatment of a lysosomal storage disease which arise from a defect in an enzyme whose activity is not directly associated with the presence of lysosomal BMP as a co-factor; such as glycogen storage diseases, gangliosidoses, neuronal ceroid lipofuscinoses, cerebrotendinous cholesterosis, Wolman's disease, cholesteryl ester storage disease, disorders of glycosaminoglycan metabolism, mucopolysaccharidoses, disorders of glycoprotein metabolism, mucolipidoses, aspartylglucosaminuria, fucosidosis, mannosidoses, and sialidosis type II.
    Type: Grant
    Filed: May 19, 2017
    Date of Patent: January 14, 2020
    Assignee: Orphazyme A/S
    Inventors: Thomas K. Jensen, Anders Mørkeberg Hinsby
  • Patent number: 10526600
    Abstract: Microcavity arrays and methods for quantitative biochemical and biophysical analysis of populations of biological variants. Examples include high-throughput analysis of cells and protein products use a range of fluorescent assays, including binding-affinity measurement and time-resolved enzyme assays. Laser-based extraction of microcavity contents.
    Type: Grant
    Filed: February 22, 2016
    Date of Patent: January 7, 2020
    Assignee: The Board of Trustees of the Leland Stanford Junior University
    Inventors: Thomas M. Baer, Jennifer R. Cochran, Bob Chen, Spencer Caleb Alford, Ivan Dimov
  • Patent number: 10509036
    Abstract: Provided herein are methods for selecting/identifying and/or isolating cancer stem cells from a biological sample or a cell culture sample using a fluorescent glucose analog. Also provided herein are methods for selecting/identifying and/or isolating leukemia stem cells and subpopulations thereof. The present invention is based, in part, on the discovery that cancer stem cells can be selected/identified based upon a lower level of fluorescence of the fluorescent glucose analog compared to non-cancer stem cells and that specific genes are differentially expressed in leukemia stem cells compared to non-leukemia stem cells.
    Type: Grant
    Filed: May 4, 2017
    Date of Patent: December 17, 2019
    Assignee: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
    Inventors: Noriko Satake, Nitin Nitin
  • Patent number: 10502685
    Abstract: The present invention relates to a method for analyzing urine sample, a reagent for analysis of urine sample and a reagent kit for analysis of urine sample, which are for detecting at least casts and erythrocytes as urinary particles.
    Type: Grant
    Filed: February 27, 2015
    Date of Patent: December 10, 2019
    Assignee: SYSMEX CORPORATION
    Inventor: Akinori Kawai
  • Patent number: 10495563
    Abstract: Reader observation and monitoring of the imaging of biological development is shown and described. In one embodiment, a method for monitoring biological growth includes identifying an alert from comparing a background value identified on a plate to a non-background value identified on a plate. In particular examples, a pre-filter, threshold, and histogram analysis identifies an alert count.
    Type: Grant
    Filed: April 28, 2017
    Date of Patent: December 3, 2019
    Assignee: Charm Sciences, Inc.
    Inventors: Richard T. Skiffington, Robert S. Salter, Robert J. Markovsky, Stanley E. Charm
  • Patent number: 10494638
    Abstract: The present disclosure provides methods and kits for generating recombinant bacteriophage genomes. Specifically, the present technology provides methods of integrating a heterologous nucleic acid sequence into a bacteriophage DNA genome, and isolating recombinant bacteriophages that express the heterologous nucleic acid sequence.
    Type: Grant
    Filed: February 9, 2018
    Date of Patent: December 3, 2019
    Assignee: The Charles Stark Draper Laboratory, Inc.
    Inventors: Jason Holder, Connor McBrine, Miles Rogers, Nicole Billings, Sarah Gruska, Lucas Tilley
  • Patent number: 10472662
    Abstract: Presented herein are solid supports on or in which a composition comprising a lysis reagent and an intracellular microorganism target detection reagent (e.g., an intracellular bacterial target detection reagent) is present in a dried form. Presented herein are also methods for detecting the presence of an intracellular microorganism target (e.g., an intracellular bacterial target) utilizing such solid supports. Further, presented herein are tablets as well as dry powders comprising a lysis reagent and an intracellular microorganism target detection reagent (e.g., an intracellular bacterial target detection reagent), and methods of using such tablets and dry powders to detect the presence of an intracellular microorganism target (e.g., an intracellular bacterial target).
    Type: Grant
    Filed: December 1, 2017
    Date of Patent: November 12, 2019
    Assignee: Becton, Dickinson and Company
    Inventors: Mei Yang-Woytowitz, Charles Yu, Timothy Wiles
  • Patent number: 10475527
    Abstract: A method for determining whether microbial growth is occurring within a specimen container includes steps of incubating the specimen container and obtaining a series of measurement data points while the specimen container is incubated and storing the data points in a machine-readable memory. The series of measurement data points represent a growth curve of microbial growth within the specimen container. The methods determine a positive condition of microbial growth within the container from the measurement data points.
    Type: Grant
    Filed: March 7, 2013
    Date of Patent: November 12, 2019
    Assignee: bioMerieux, Inc.
    Inventor: Michael Ullery
  • Patent number: 10465231
    Abstract: A method and an apparatus are provided for enriching pathogen DNA. The method includes adding a selective lysis buffer to a sample, incubating the mixture formed thereby, and filtering the mixture. The apparatus for enriching pathogen DNA includes: a lysis chamber; a reservoir containing selective lysis buffer, connected to the lysis chamber; and a filter connected to the lysis chamber; that achieves a limit of detection of pathogens of 500 cfu/ml or less.
    Type: Grant
    Filed: March 15, 2017
    Date of Patent: November 5, 2019
    Assignee: Siemens Healthcare GmbH
    Inventors: Divya Khandige Sharma, Ragavendar MS, Nivedita Mitra, Ramya Vutukuru, Yiwei Huang
  • Patent number: 10457908
    Abstract: The present invention relates to a method of analysis comprising the preparation and the analysis of a sample in a flexible container without direct handling of the sample and without reopening the flexible container at the end of the preparation of the sample, and also to a container and a kit that enable the implementation of this method.
    Type: Grant
    Filed: April 3, 2015
    Date of Patent: October 29, 2019
    Assignees: CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE-CNRS, PRESTODIAG, COMMISSARIAT A L'ENERGIE ATOMIQUE ET AUX ENERGIES ALTERNATIVES
    Inventors: Roberto Calemczuk, David Carrara, Thierry Livache, Thibaut Mercey, Félix Piat, Yoann Roupioz, Sami Slimani
  • Patent number: 10451638
    Abstract: Disclosed is a blood analyzer that includes: a sample preparation unit configured to mix a blood specimen and a fluorescent dye for staining nucleic acid to prepare a measurement sample; a light receiver configured to receive fluorescence and scattered light that are generated by light being applied to the measurement sample prepared by the sample preparation unit; and a controller programmed to determine whether or not infection with Plasmodium has occurred, on the basis of a value representing variation of a distribution of first particles in a range where single-ring form of red blood cells appear, the range where single-ring form of red blood cells appear being identified according to fluorescence intensities and scattered light intensities obtained by processing of signals from the light receiver.
    Type: Grant
    Filed: July 24, 2017
    Date of Patent: October 22, 2019
    Assignee: SYSMEX CORPORATION
    Inventors: Yuhgi Suzuki, Takuma Watanabe
  • Patent number: 10443084
    Abstract: Conjugates of 2,3-dihydroxynaphthalene and its derivatives with enzyme cleavable groups are chromogenic substrates that form colored compounds when complexed with metal ions, e.g. iron ions, on cleavage by enzymes, and are useful in microbial detection and identification. The cleavage products form purple or red-brown colored complexes, that can easily be observed by the naked eye. Microbes can be grown in the presence of the substrates and the metal salts that provide the metal ion for complexing with the 2,3-dihydroxynaphthalene product. Substituents in the naphthalene ring may affect the solubility of the substrates and also the diffusibility and color of the metal complexes. Some of the substrates yield soluble complexes on cleavage and are of particular value in liquid growth media. Other substrates produce less soluble complexes that are more suitable for use in solid agar media.
    Type: Grant
    Filed: February 27, 2018
    Date of Patent: October 15, 2019
    Assignee: GLYCOSYNTH LIMITED
    Inventors: Hayley Jane Turner, Michael Burton
  • Patent number: 10415075
    Abstract: A method is described to separate, characterize and/or identify microorganisms in a test sample. Said method comprises the steps of (i) lysing non-microorganism cells that might be present in the sample, (ii) separating the microorganisms as a pellet in the sample post-lysing non-microorganism cells, (iii) interrogating the pellet containing microorganisms by mass spectrometry to produce mass spectra of microorganisms, and (iv) characterizing and/or identifying microorganisms in the sample. The method may be used in characterizing/identifying microorganisms in complex samples, e.g., blood containing culture media.
    Type: Grant
    Filed: October 30, 2009
    Date of Patent: September 17, 2019
    Assignee: bioMerieux, Inc.
    Inventors: Jones Hyman, Bradford Clay, John Walsh, Thurman Thorpe
  • Patent number: 10415081
    Abstract: The invention provides methods and processes for the identification of polymorphisms at one ore more designated sites, without interference from non-designated sites located within proximity of such designated sites. Probes are provided capable of interrogation of such designated sites in order to determine the composition of each such designated site. By the methods of this invention, one ore more mutations within the CFTR gene and the HLA gene complex can be identified.
    Type: Grant
    Filed: August 4, 2016
    Date of Patent: September 17, 2019
    Assignee: BioArray Solutions Ltd.
    Inventors: Alice Xiang Li, Ghazala Hashmi, Michael Seul
  • Patent number: 10413592
    Abstract: The present invention relates to improved assisted reproductive technology using highly purified menotropin (HP-hMG) to stimulate follicle development in controlled ovarian stimulation, particularly in women at risk of a high ovarian response to controlled ovarian stimulation.
    Type: Grant
    Filed: February 25, 2016
    Date of Patent: September 17, 2019
    Assignee: FERRING B.V.
    Inventors: Joan-Carles Arce, Jane Ruman
  • Patent number: 10406227
    Abstract: Methods and compositions modulate the activity of electrically excitable cells. Photovoltaic compounds which, upon exposure to light energy, increase or decrease the electrical activity of cells. These supplement and/or replace of vision based on the conversion of light energy to electrical energy within certain cells of the visual system. A “patch” or bridge to circumvent one or more defective, damaged, or diseased cells in the visual system. Additionally, in several embodiments, subjects with normal vision can benefit from the methods, compositions, systems, and/or devices disclosed herein as normal visual acuity can be heightened. The exposure induces an energy (e.g., a receipt of light energy, conversion to electrical energy, and passage of that electrical energy) from the photovoltaic compound to the cell, thereby altering the transmembrane potential of the cell and/or the opening of one or more ion channels, thereby modulating the activity of the electrically excitable cell.
    Type: Grant
    Filed: December 18, 2013
    Date of Patent: September 10, 2019
    Assignees: California Institute of Technology, University of Southern California
    Inventors: Robert H. Chow, Mark S. Humayun, Harry B. Gray, Robert H. Grubbs, Dennis A. Dougherty, Mark E. Thompson, Lionel E. Cheruzel, Melanie A. Yen
  • Patent number: 10408735
    Abstract: An in vitro method for the marker-free determination of a cell type of a cell in a biological sample is disclosed, a microscopic device being configured to detect a height profile of the cell with respect to a carrier plate. An embodiment of the method performed by a cell analysis device, include: determining a cell compartment of the cell on the basis of the detected height profile, determining a predetermined quantitative cell feature on the basis of the determined cell compartment, and determining the cell type of the cell on the basis of the determined quantitative cell feature. A correspondingly designed cell analysis device and a corresponding microscopic device are also disclosed.
    Type: Grant
    Filed: September 30, 2014
    Date of Patent: September 10, 2019
    Assignee: Siemens Healthcare GmbH
    Inventors: Oliver Hayden, Oliver Schmidt
  • Patent number: 10392648
    Abstract: Provided are an airborne microbial measurement apparatus and a method of measuring the same. The airborne microbial measurement apparatus includes a particle separation device including a main body having a flow space in which airborne microorganism flows and a collection unit separably coupled to one side of the main body to collect the airborne microorganism, a reagent container in which a lysis reagent reacting with the airborne microorganism collected in the collection unit and a luminous material are stored, and a luminescence measurement device for measuring intensity of light emitted after the airborne microorganism reacts with the lysis reagent and the luminous material.
    Type: Grant
    Filed: December 16, 2014
    Date of Patent: August 27, 2019
    Assignees: LG ELECTRONICS INC., INDUSTRY-ACADEMIC COOPERATION FOUNDATION, YONSEI UNIVERSITY
    Inventors: Chulwoo Park, Yeekyeong Jung, Sunghwa Lee, Jungho Hwang, Jiwoon Park
  • Patent number: 10386376
    Abstract: A test device for detection and visual identification of a specific analyte in a liquid sample such as a bodily fluid is disclosed. The device includes a collection container, a test strip affixed to an interior surface of the collection container, and a removable protective strip adhered over the test strip. The protective strip is configured to be removed from contact with the test strip after the liquid sample has been collected in order to prevent unnecessary exposure and/or contamination of the test strip. In certain embodiments, the protective strip may be removed from the contact with the test strip when the collection container is in a sealed configuration. In other embodiments, the protective strip may be dissolvable when placed in contact with the desired liquid sample. An assay specific to the anticipated analyte may be provided on the test strip.
    Type: Grant
    Filed: November 9, 2015
    Date of Patent: August 20, 2019
    Assignee: JEIMEI, LLC
    Inventor: Christina Holloway
  • Patent number: 10380807
    Abstract: A pipette check station for checking the calibration or service status of a pipette includes an RFID reader, a user interface with a display and buttons, and a digital interface to connect the check station to additional equipment; the check station optionally further includes provisions to hold pipettes for storage and to charge electronic pipettes held thereupon.
    Type: Grant
    Filed: January 1, 2018
    Date of Patent: August 13, 2019
    Assignee: Mettler-Toledo Rainin, LLC
    Inventors: Gerhard Keller, Richard Hill, Deryl Stanley, James Petrek, Murray Anderson
  • Patent number: 10352934
    Abstract: A test substrate for detecting a LAL-reactive substance, wherein at least a portion of said test substrate has been preloaded with at least one LAL reagent and/or at least one LAL-reactive standard. Methods of use of the test substrate are disclosed. Methods of depositing test reagents on a test substrate are also disclosed.
    Type: Grant
    Filed: September 28, 2017
    Date of Patent: July 16, 2019
    Assignee: GENERAL ELECTRIC COMPANY
    Inventors: Richard Douglas Godec, Paul Charles Melanson, Matthew Kaddeland Stonesmith, Hong Xu, Yan Huang
  • Patent number: 10351896
    Abstract: Use of at least one chromogenic and/or fluorogenic carboxylesterase and/or triacylglycerol-lipase substrate, to detect bacteria of the Bacillus cereus group in a sample capable of containing them.
    Type: Grant
    Filed: December 23, 2014
    Date of Patent: July 16, 2019
    Assignee: BIOMERIEUX
    Inventors: Marie Cellier, Marie-Pierre Bourguignon, Diane Halimi
  • Patent number: 10345259
    Abstract: The present invention provides a method for the quantification of virus particles in a biological sample, comprising the steps of: (a) introducing said biological sample comprising virus particles into a capillary tube containing a buffer solution; (b) applying an electrical field to said capillary tube of sufficient voltage to allow for the separation of the virus particles from additional constituents in said sample, to obtain electrophoretical fractions; (c) generating an electropherogram associated with the electrophoretical fractions; and (d) determining the concentration of virus particles in said sample by comparing the electropherogram with an electropherogram generated from a reference sample containing a known concentration of said virus particles.
    Type: Grant
    Filed: February 18, 2016
    Date of Patent: July 9, 2019
    Assignee: Janssen Vaccines & Prevention B.V.
    Inventors: Ewoud Van Tricht, Lars Johannes Geurink, Catharina Elise Sänger-Van De Griend
  • Patent number: 10330584
    Abstract: A cell analyzer and a particle sorting method and device are disclosed. The method comprises: acquiring a pulse width of at least one optical signal according to a detected optical signal, selecting at least one optical signal as a combined optical signal, and respectively calculating a signal intensity of the combined optical signal with the pulse width in a combinatorial way to obtain at least one reinforcement signal, where a difference between a first category of particles and a second categories of particles in the reinforcement signal is increased relative to a difference therebetween in the combined optical signal; and on the basis of the reinforcement signal and at least another signal, forming a new scatter diagram, where the at least another signal is one of other reinforcement signals and the optical signal, distinguishing the first category of particles from the second category of particles according to the new scatter diagram.
    Type: Grant
    Filed: August 11, 2017
    Date of Patent: June 25, 2019
    Assignee: Shenzhen Mindray Bio-Medical Electronics Co., Ltd.
    Inventors: Bo Ye, Guanzhen Wang, Jiantao Di, Ying Zhang, Huan Qi
  • Patent number: 10311573
    Abstract: Systems, methods, and devices for classifying or detecting mold samples or training computer models (such as neural networks), are disclosed. A method includes obtaining a microscopy image of a mold sample. The method includes determining a classification of the mold sample based on non-image data corresponding to the mold sample. The method further includes training a computer model based on the microscopy image and a label indicating the classification.
    Type: Grant
    Filed: May 2, 2017
    Date of Patent: June 4, 2019
    Assignee: Techcyte, Inc.
    Inventors: S. Russell Zimmerman, Ralph Yarro, III, Benjamin P. Cahoon, Richard Boyd Smith, Hyrum S. Anderson
  • Patent number: 10288569
    Abstract: A method for preparing a hydrogel that reversibly changes color depending on pH includes: (S10) dissolving a carboxyl group-containing polysaccharide in buffer to obtain a polysaccharide solution; (S20) adding a cross-linker solution composed of a mixture of an organic solvent and a cross-linker to the polysaccharide solution; (S30) adding, to the cross-linker-containing polysaccharide solution resulting from step (S20), an organic solution composed of a mixture of an organic solvent and a first organic compound containing an aromatic functional group having at least one hydroxyl group bonded thereto, to form a mixture solution, and allowing the mixture solution to react; (S40) obtaining a polymer complex from the reaction mixture resulting from step (S30); and (S50) mixing the polymer complex from step (S40) with an organic dye comprising a second organic compound containing an aromatic functional group having at least one hydroxyl group bonded thereto.
    Type: Grant
    Filed: February 8, 2017
    Date of Patent: May 14, 2019
    Assignee: INDUSTRY-ACADEMIC CORPORATION FOUNDATION YONSEI UNIVERSITY
    Inventors: Sang Yup Lee, Chaemyeong Lee, Min Chul Kim, Jin Young Kwak, Sang Woo Park, Young Jun Lim
  • Patent number: 10280446
    Abstract: The disclosure provides articles and methods useful for detecting a discrete source of DNase activity. DNase-producing microorganisms can be detected. The device can further include selective agents and/or indicators to differentiate groups or species microorganisms. Methods of use include detecting or enumerating DNase-producing microorganisms.
    Type: Grant
    Filed: July 17, 2017
    Date of Patent: May 7, 2019
    Assignee: 3M Innovative Properties Company
    Inventors: Patrick A. Mach, Michelle L. Rosauer, Michael E. Hughes
  • Patent number: 10260082
    Abstract: Liposomal detection devices and methods of making and using such devices are disclosed. Such liposomal detection devices may be used to detect microbes by detecting a byproduct of microbial metabolism, or may be used to detect changes in pH and/or changes in temperature. Liposomal detection devices may include a first liposome encapsulating a first destabilizing compound and a second liposome encapsulating a second destabilizing compound. The first liposome may destabilize in response to the detection of the target compound or change to release the first destabilizing compound which may destabilize the second liposome. A matrix, such as a hydrogel matrix, may encase the first liposome and the second liposome.
    Type: Grant
    Filed: October 22, 2013
    Date of Patent: April 16, 2019
    Assignee: EMPIRE TECHNOLOGY DEVELOPMENT LLC
    Inventors: Michael Keoni Manion, George Charles Peppou
  • Patent number: 10233476
    Abstract: An apparatus (1) for analyzing a contaminated surface has a transfer device (2) with a mount (3) and a porous disk-shaped medium (5) having a contact side (6) arrangeable on the contaminated surface. The mount (3) is bindable to the medium on its side (8) facing away from the contact side (6) via a first fixing edge (7a). An analytical device (11) is bindable to the medium (5) on its contact side (6) via a second fixing edge (7b) to remove the medium (5) from the mount (3). The first fixing edge (7a) is bindable to the medium (5) via a first adhesive bond and the second fixing edge (7b) is bindable to the medium (5) via a second adhesive bond. The first adhesive bond is breakable by a lower application of force than the second adhesive bond.
    Type: Grant
    Filed: June 8, 2011
    Date of Patent: March 19, 2019
    Assignee: SARTORIUS STEDIM BIOTECH GMBH
    Inventor: Karl Pflanz
  • Patent number: 10228312
    Abstract: The present invention relates to the chromatographic isolation of a target cell or another complex biological material, in particular by column chromatography such as affinity chromatography or gel permeation chromatography. The invention employs a receptor binding reagent that binds to a receptor molecule that is located on the surface of a target cell. The invention in general provides novel methods for the traceless isolation of biologic materials such as cells, cell organelles, viruses and the like. The invention also relates to an apparatus for the isolation of cells and other complex biological materials.
    Type: Grant
    Filed: February 25, 2013
    Date of Patent: March 12, 2019
    Assignee: Juno Therapeutics GmbH
    Inventor: Herbert Stadler