Determining Presence Or Kind Of Micro-organism; Use Of Selective Media Patents (Class 435/34)
  • Patent number: 11225681
    Abstract: Various embodiments disclosed herein provide for reagents and methods for rapidly isolating viable microbial cells, including S. pneumoniae, from positive blood culture samples. The resulting microbial pellet can be used for both identification and growth-based methods such as antimicrobial susceptibility testing. The buffers described herein may contain a base solution, non-ionic detergents, thiols, and optionally, ammonium chloride. The disclosed methods provide a process for rapidly isolating and concentrating viable microorganism(s) from PBC samples using only one sample preparation tube and centrifugation while removing cellular debris from the mammalian blood cells that may interfere with identification methods.
    Type: Grant
    Filed: November 7, 2019
    Date of Patent: January 18, 2022
    Assignee: BECTON, DICKINSON AND COMPANY
    Inventors: Susan M. Kircher, Vanda White, William B. Brasso, Dyan Luper, James Y. Zhou, Julie L. Rosales, Jeffery H. Bruton, John D. Mantlo, Adrien P. Malick, Donald R. Callihan, Ben Turng, Liping Feng, Curtis M. Gosnell, Patrick Shawn Beaty, John P. Douglass
  • Patent number: 11199541
    Abstract: According to some aspects, a method for analyzing a cell is provided. The method includes trapping the cell by binding a first molecule to the cell. The method further includes binding a second molecule to the cell. The second molecule includes a binding portion capable of specific binding to a cell-surface molecule of the cell. The second molecule further includes an identifying portion, a labeling portion coupled to the identifying portion, and a stimulus-degradable linker between the binding portion and the identification portion. The method further includes detaching the identifying portion from the binding portion by stimulating the stimulus-degradable linker where the detached identifying portion is coupled to the labeling portion. The method further includes binding the detached identifying portion through specific binding to an identifying portion recognizing molecule and detecting the labeling portion.
    Type: Grant
    Filed: August 4, 2016
    Date of Patent: December 14, 2021
    Assignee: Sony Corporation
    Inventors: Masahiro Matsumoto, Masataka Shinoda, Yuuki Watanabe
  • Patent number: 11193103
    Abstract: A method of controlling a bioreactor system includes providing a cell culture in a bioreactor, wherein conditions in the bioreactor enable the cell culture to produce a protein of interest (POI), measuring process parameters (PPs) of the culture within the bioreactor by RAMAN, wherein the process parameters are selected from the group consisting of nutrient concentration, viable cell concentration, and protein attributes, measuring a predetermined weight of the bioreactor with the cell culture, removing cell-free spent media from the cell culture using a first output conduit at a first specified rate, removing cells from the cell culture using a second output conduit at a second specified rate, and introducing one or both of fresh media or nutrients into the cell culture using an input conduit at a third specified rate.
    Type: Grant
    Filed: October 15, 2018
    Date of Patent: December 7, 2021
    Assignee: Regeneran Pharmaceuticals, Inc.
    Inventors: Matthew Angelini, Ashley Witmer, Anthony Debiase
  • Patent number: 11193158
    Abstract: Rapid methods that identify sepsis-causing bacteria or yeast aid the physician in critical therapeutic decision-making, thus decreasing patient mortality rates. The methods described herein employ plating microorganisms directly on to a MALDI-MS plate, adding concentrated formic acid, and identifying the microorganism by mass spectrometry. Optionally, an organic solvent may be combined with the formic acid, or added to the sample before or after the concentrated formic acid is added thereto. The methods enable direct extraction of proteins from microorganisms without the need for liquid protein extraction methods and yields positive identification results for gram-positive bacteria, gram-negative bacteria and yeast in minutes.
    Type: Grant
    Filed: January 6, 2020
    Date of Patent: December 7, 2021
    Assignee: BECTON, DICKINSON AND COMPANY
    Inventors: Liping Feng, William B. Brasso, Susan M. Kircher, Vanda White, Song Shi, Xiao Mo, Tuan-Linh Ngoc Nguyen, Adrien P. Malick, Jon E. Salomon, John D. Mantlo
  • Patent number: 11180735
    Abstract: Functional in vitro assays are provided for determining patient specific responsiveness to immunotherapy agents within a clinically actionable time frame.
    Type: Grant
    Filed: October 25, 2017
    Date of Patent: November 23, 2021
    Assignee: The Board of Trustees of the Leland Stanford Junior University
    Inventors: James Neal, Calvin Jay Kuo
  • Patent number: 11169484
    Abstract: A cell visualization system includes a digital holographic microscopy (DHM) device, a training device, and a virtual staining device. The DHM device produces DHM images of cells and the virtual staining device colorizes the DHM images based on an algorithm generated by the training device using generative adversarial networks and unpaired training data. A computer-implemented method for producing a virtually stained DHM image includes acquiring an image conversion algorithm which was trained using the generative adversarial networks, receiving a DHM image with depictions of one or more cells and virtually staining the DHM image by processing the DHM image using the image conversion algorithm. The virtually stained DHM image includes digital colorization of the one or more cells to imitate the appearance of a corresponding actually stained cell.
    Type: Grant
    Filed: September 6, 2018
    Date of Patent: November 9, 2021
    Assignee: Siemens Healthcare Diagnostics Inc.
    Inventors: Noha Youssry El-Zehiry, Saikiran Rapaka, Ali Kamen
  • Patent number: 11162852
    Abstract: A sensor to sense pressure in a pressure bottle and a method of assembling the sensor involve two or more fiber Bragg gratings (FBGs) affixed to a different radial location of a diaphragm seal of the pressure bottle. The sensor includes a light source to provide incident light to the two or more FBGs, and a photodetector to detect reflected light resulting from the two or more FBGs. Processing circuitry determines a pressure change in the pressure bottle based on the reflected light resulting from each of the two or more FBGs.
    Type: Grant
    Filed: September 8, 2020
    Date of Patent: November 2, 2021
    Assignee: KIDDE TECHNOLOGIES, INC.
    Inventors: Elbert Jeyapaul, Aswin Kumar Vallamkondu
  • Patent number: 11163048
    Abstract: Various sensors, sensor controllers, and sensor control methods are provided with model-based sideband balancing. In one illustrative embodiment, a controller for a piezoelectric transducer includes a transmitter, a receiver, and a processing circuit coupled to the transmitter and receiver. The processing circuit performs calibration and echo detection, the calibration including: sensing the piezoelectric transducer's phase response as a function of frequency; deriving equivalent circuit parameters for the piezoelectric transducer from the phase response; and determining a sideband imbalance based on one or more of the equivalent circuit parameters. Once the sideband imbalance is identified, the processing circuit may perform echo-detection processing that accounts for the sideband imbalance.
    Type: Grant
    Filed: December 23, 2019
    Date of Patent: November 2, 2021
    Assignee: SEMICONDUCTOR COMPONENTS INDUSTRIES, LLC
    Inventors: Tomas Suchy, Jiri Kantor, Marek Hustava
  • Patent number: 11160893
    Abstract: A method for controlling infections in a facility such as a hospital or foodservice establishment is provided. The method comprises tagging assets, monitoring asset location and pathogen contamination for each asset over time, analyzing data sets to identify which assets are the critical control points for pathogen transfer, and coating a residual self-sanitizing coating composition on each asset identified as a critical control point. The infection control method shuts down pathogen transfer routes by mitigating or eliminating pathogen growth on the critical control points.
    Type: Grant
    Filed: June 20, 2018
    Date of Patent: November 2, 2021
    Assignee: Allied Bioscience, Inc.
    Inventors: Craig Grossman, Ingrida Grossman, Gavri Grossman
  • Patent number: 11149268
    Abstract: The present invention is based on the advantageous use of single-stranded oligonucleotides in the in vivo (within a cell) assembly of double-stranded oligonucleotides into a single double-stranded nucleic acid construct. The present invention relates to the use of at least a first and a second single-stranded oligonucleotide in the assembly within a cell of at least two double-stranded nucleic acid molecules into a single double-stranded nucleic acid construct of pre-determined sequence, wherein the first and second single-stranded oligonucleotide are essentially complementary to each other.
    Type: Grant
    Filed: December 15, 2016
    Date of Patent: October 19, 2021
    Assignee: DSM IP ASSETS B.V.
    Inventors: Johannes Andries Roubos, René Verwaal, Bianca Elisabeth Maria Gielesen, Brenda Vonk
  • Patent number: 11105742
    Abstract: Disclosed are nucleated red blood cell warning devices and methods, and flow cytometers using the same. The devices, methods and flow cytometers can warn whether nucleated red blood cells exist in a blood sample. The warning device may obtain forward-scattered light information, side-scattered light information and fluorescence information when cells in the blood sample pass through a detection region of the flow cytometer. The warning device may generate a side-scattered light-fluorescence dot plot, so as to classify leucocytes into four groups, and may generate a forward-scattered light-fluorescence dot plot, where the forward-scattered light-fluorescence dot plot can include a leucocyte group region. The warning device may obtain a predetermined feature region located at the left side of the leucocyte group region, perform statistics on the amount of characterization cells in the predetermined feature region, and provide a warning when the number of the characterization cells exceeds a threshold value.
    Type: Grant
    Filed: June 30, 2017
    Date of Patent: August 31, 2021
    Assignee: Shenzhen Mindray Bio-Medical Electronics Co., Ltd.
    Inventors: Bo Ye, Huan Qi, Cheng Qian
  • Patent number: 11098341
    Abstract: A method for determining whether a test sample contains a phytopathogenic fungus, which includes (a) putting the test sample on a front surface of a substrate having a through hole; the substrate having a cellulose film on the back surface thereof; the through hole has a cross-sectional area of not less than 7.065 square micrometers and not more than 19.625 square micrometers; and the cellulose film has a thickness of not less than 0.5 micrometers and not more than 3.7 micrometers; (b) leaving the test sample at rest after the step (a); (c) irradiating the cellulose film with ultraviolet light after the step (b); (d) bringing a back surface of the cellulose film into contact with a fungus color reaction reagent after the step (c); and (e) determining that the test sample contains the phytopathogenic fungus, if a color is given to the fungus color reaction reagent.
    Type: Grant
    Filed: August 22, 2019
    Date of Patent: August 24, 2021
    Assignee: PANASONIC INTELLECTUAL PROPERTY MANAGEMENT CO., LTD.
    Inventors: Yuki Kushida, Yoshitsugu Uriu, Yosifumi Kariatumari
  • Patent number: 11098274
    Abstract: Provided are a method for detecting a free rare tumor cell in a human biological fluid sample and assay kit. Specifically, the method of the invention determines the existence of an active tumor cell in a biological fluid sample of a cancer patient by detecting and confirming the existence of glucose absorption capability of a karyocyte and expression of a leukocyte marker (CD45).
    Type: Grant
    Filed: April 29, 2016
    Date of Patent: August 24, 2021
    Assignee: SHANGHAI QIANYI BIOTECH CO. LTD.
    Inventors: Qihui Shi, Shun Lu, Yuliang Deng, Yin Tang
  • Patent number: 11083053
    Abstract: A process for heating articles in a heating system includes passing an article in a carrier through a heating chamber that is at least partially filled with a liquid medium to form a liquid bath. The process further includes heating the article in the carrier by at least partially submerging the article into the liquid bath during heating, the heating being performed, at least in part, using microwave energy. The process further includes one or more of adding fluid into and removing fluid from at least one location in the heating chamber to maintain a temperature profile across the heating chamber. In one implementation, the temperature of the liquid bath at an inlet area of the heating chamber is at least 10° C. cooler than a temperature of the liquid bath at an outlet area of the heating chamber.
    Type: Grant
    Filed: October 19, 2018
    Date of Patent: August 3, 2021
    Assignee: 915 LABS, INC.
    Inventor: Harold Dail Kimrey, Jr.
  • Patent number: 11079395
    Abstract: Provided herein are methods for predicting or determining whether a subject with coronary artery disease is likely to experience or develop a major adverse cardiovascular event (MACE) based on determining cardiac troponin (cTnI) levels in a human at rest and during or after exercise.
    Type: Grant
    Filed: February 28, 2020
    Date of Patent: August 3, 2021
    Assignees: Abbott Laboratories, Emory University
    Inventors: Agim Beshiri, Arshed A. Quyyumi
  • Patent number: 11077127
    Abstract: The invention relates to a nutritional adjuvant, comprising baicalin and/or baicalein diluted on a medium, and to a feed comprising such an adjuvant, for improving production performance in farm animals in stressful situations.
    Type: Grant
    Filed: August 28, 2019
    Date of Patent: August 3, 2021
    Assignee: DELTAVIT
    Inventor: Fabrice Robert
  • Patent number: 11072775
    Abstract: The present application relates to systems and methods for transporting biological samples, including a transport medium configured to protect bacterial viability and optimize sample quality, for enhanced diagnostic accuracy. The disclosed systems and methods can include a transport media, a collection tube including a screen and sampling brush, and a transport case. The disclosed systems and methods allow samples to be frozen and thawed while preserving cellular wall integrity during transport or storage, and furthermore can preserve the survival of the bacterial sample, enabling more accurate detection of an infectious agent.
    Type: Grant
    Filed: July 9, 2020
    Date of Patent: July 27, 2021
    Assignee: Biosherpa, LLC
    Inventors: Thomas A. Cumbo, Christopher L. Hutson, Destin J. Radder
  • Patent number: 11007528
    Abstract: Systems, methods, and devices for determining various properties of cells are provided herein. In one aspect, the method includes measuring one or more variables based on physical and/or chemical characteristics of a cell or its environment. The method further includes calculating one or more quantifiable metrics based on a mathematical expression including one or more of the variables, where those metrics correlate with at least one of the growth characteristics of the cell, the motility characteristics of the cell, the oncogenic potential of the cell, or the metastatic potential of the cell. Various aspects of systems and devices for determining various properties of cells are also provided herein.
    Type: Grant
    Filed: June 21, 2017
    Date of Patent: May 18, 2021
    Assignee: CELLANYX DIAGNOSTICS, LLC
    Inventor: Ashok C. Chander
  • Patent number: 11001873
    Abstract: A microorganism detection medium, said detection being based on showing the presence of a microbial enzyme activity chosen from esterase and/or osidase and/or peptidase and/or sulfatase and/or phosphatase activities of microorganisms, preferably said microbial enzyme activity being an esterase activity, said medium comprising: at least one chromogenic and/or fluorogenic substrate specific to the enzyme activity sought, preferably specific to an esterase activity, at least one alkyl(thio)glycoside, at least one solvent (S).
    Type: Grant
    Filed: August 28, 2018
    Date of Patent: May 11, 2021
    Assignee: BIOMERIEUX
    Inventors: Marie Cellier, Sylvain Orenga, John Perry
  • Patent number: 10983052
    Abstract: An electricity measuring type surface plasmon resonance sensor including: a plasmon polariton intensifying sensor chip in which a prism and a sensor chip including a transparent electrode, an n-type transparent semiconductor film, and a plasmon resonance film electrode arranged in this order are arranged in an order of the prism, the transparent electrode, the n-type transparent semiconductor film, and the plasmon resonance film electrode; and an electric measuring apparatus which directly measures a current or voltage from the transparent electrode and the plasmon resonance film electrode.
    Type: Grant
    Filed: August 9, 2018
    Date of Patent: April 20, 2021
    Assignees: IMRA JAPAN KABUSHIKIKAISHA, NATIONAL UNIVERSITY CORPORATION HOKKAIDO UNIVERSITY
    Inventors: Hironori Suzuki, Giles Allison, Masanori Sasaki, Koki Hayashi, Hiroaki Misawa, Kosei Ueno
  • Patent number: 10983042
    Abstract: A cell analyzer and a sorting method for the cell analyzer are disclosed. Multiple optical signals generated by each of particles irradiated with light in a blood sample in a detection region are collected. The particles includes a first category of particles and a second category of particles. For each of the particles, Intensities of a first group of optical signals, which includes at least two optical signals selected from the multiple optical signals, and a pulse width of a second group of optical signals, which includes at least one optical signal selected from the multiple optical signals are acquired. For each of the particles, one or more reinforcement signals related to the particle are calculated based on an intensity of a first optical signal selected from the first group of optical signals and a pulse width of a second optical signal selected from the second group of optical signals, where the first optical signal is as same as or different from the second optical signal.
    Type: Grant
    Filed: February 26, 2020
    Date of Patent: April 20, 2021
    Assignee: Shenzhen Mindray Bio-Medical Electronics Co., Ltd
    Inventors: Bo Ye, Guanzhen Wang, Jiantao Di, Ying Zhang, Huan Qi
  • Patent number: 10969332
    Abstract: Exemplary methods can be provided for the determination of a microbial pathogen. In addition, exemplary methods can be provided for the determination of a microbial pathogen and its anti-infective resistance. Further, an exemplary method can be provided for determining a bacterium and its antibiotic resistance. Systems and computer-accessible media can be provided for the determination of a microbial pathogen and its anti-infective resistance.
    Type: Grant
    Filed: July 26, 2017
    Date of Patent: April 6, 2021
    Assignees: LEIBNIZ-INSTITUT FÜR PHOTONISCHE TECHNOLOGIEN E.V., UNIVERSITÄTSKLINIKUM JENA
    Inventors: Ute Neugebauer, Jürgen Popp, Ondrej Stranik, Bettina Löffler
  • Patent number: 10968473
    Abstract: A method and a system for determining the presence of at least one determined microorganism in a Petri dish comprising one or more colonies of microorganisms and a culture medium, and a computer program product which, when executed, allows the method of the invention to be performed.
    Type: Grant
    Filed: August 30, 2017
    Date of Patent: April 6, 2021
    Assignee: Biomerieux
    Inventors: Guillaume Sutra, Philippine Barlas, Delphine Archeny, Géraldine Durand, Corine Fulchiron, Jean-Francois Gorse, Guillaume Perrin
  • Patent number: 10954529
    Abstract: The invention provides compositions and methods for reprogramming minimal volumes of mononuclear cells. In particular aspects, the invention provides methods and compositions for reprogramming minimal volumes of umbilical cord blood obtained from cord blood segments from cryopreserved cord blood segments.
    Type: Grant
    Filed: June 13, 2018
    Date of Patent: March 23, 2021
    Assignee: FATE THERAPEUTICS, INC.
    Inventors: Peter Flynn, Bahram Valamehr
  • Patent number: 10942998
    Abstract: In one aspect, a device includes a processor and storage accessible to the processor. The storage bears instructions executable by the processor to receive at least one output of a gas chromatograph (GC), compare the at least one output to at least one template, and determine whether to authenticate a user responsive to the comparison. The GC output also may be used to generate at least one advertisement targeted to the user, and may further be used to output at least one indication of at least one therapy for the user.
    Type: Grant
    Filed: May 1, 2019
    Date of Patent: March 9, 2021
    Assignee: Lenovo (Singapore) Pte. Ltd.
    Inventors: Rod D. Waltermann, Timothy Winthrop Kingsbury
  • Patent number: 10939607
    Abstract: The present invention relates to the technical field of controlling harmful organisms in the cultivation of cultivated plants. The present invention relates to a method for controlling harmful organisms, to a system for controlling harmful organisms and to the use of a digital application map for the application of one or more control agents against harmful organisms.
    Type: Grant
    Filed: June 23, 2017
    Date of Patent: March 9, 2021
    Assignee: BASF AGRO TRADEMARKS GMBH
    Inventors: Holger Hoffmann, Ole Peters, Eleen Grüneberg, Andreas Johnen, Andree-Georg Girg
  • Patent number: 10934566
    Abstract: Processes are provided for enhancing the productivity of fermenters during the metabolic conversion of methane-containing gases to products containing polyhydroxyalkanoate, which products can be used to make, for instance, animal feed or biodegradable, polymeric articles. The processes involve one or both of attenuating the heat generated to grow a population of microorganisms and removal of heat during the fermentation by removal of carbon dioxide.
    Type: Grant
    Filed: May 18, 2018
    Date of Patent: March 2, 2021
    Assignee: MANGO MATERIALS, INC.
    Inventors: Robert F. Hickey, Margaret Catherine Morse, Allison J. Pieja
  • Patent number: 10928305
    Abstract: A sensor device for quantifying luminescent targets comprises a light source, a detector, a modulator, and a processor. The light source is adapted for exciting the luminescent target. The detector is adapted for detecting the luminescence of the luminescent target resulting in a measured signal which comprises a desired signal originating from the luminescent target and a background signal. The modulator is adapted for modulating a physical parameter resulting in a modulation of the desired signal which is different from the modulation of the background signal. The processor is configured to correlate the modulation of the physical parameter with the modulation of the desired signal and/or the modulation of the background signal.
    Type: Grant
    Filed: June 30, 2016
    Date of Patent: February 23, 2021
    Assignee: IMEC VZW
    Inventors: Peter Peumans, Liesbet Lagae, Willem Van Roy, Tim Stakenborg, Pol Van Dorpe
  • Patent number: 10895533
    Abstract: Naphthalene, benzene, toluene, xylene, and other volatile organic compounds VOCs have been identified as serious health hazards. Embodiments of the invention are directed to methods and apparatus for near-real-time in-situ detection and accumulated dose measurement of exposure to naphthalene vapor and other hazardous gaseous VOCs. The methods and apparatus employ excitation of fluorophors native or endogenous to compounds of interest using light sources emitting in the ultraviolet below 300 nm and measurement of native fluorescence emissions in distinct wavebands above the excitation wavelength. The apparatus of some embodiments are cell-phone-sized sensor/dosimeter “badges” to be worn by personnel potentially exposed to hazardous VOCs. The badge sensor of some embodiments provides both real time detection and data logging of exposure to naphthalene or other VOCs of interest from which both instantaneous and accumulated dose can be determined.
    Type: Grant
    Filed: March 16, 2020
    Date of Patent: January 19, 2021
    Assignee: Photon Systems, Inc.
    Inventors: William F. Hug, Röhit Bhartia, Ray D. Reid, Arthur L. Lane
  • Patent number: 10883916
    Abstract: A cell analyzer and a sorting method for the cell analyzer are disclosed. Multiple optical signals generated by each of particles irradiated with light in a blood sample in a detection region are collected. The particles includes a first category of particles and a second category of particles. For each of the particles, Intensities of a first group of optical signals, which includes at least two optical signals selected from the multiple optical signals, and a pulse width of a second group of optical signals, which includes at least one optical signal selected from the multiple optical signals are acquired. For each of the particles, one or more reinforcement signals related to the particle are calculated based on an intensity of a first optical signal selected from the first group of optical signals and a pulse width of a second optical signal selected from the second group of optical signals, where the first optical signal is as same as or different from the second optical signal.
    Type: Grant
    Filed: February 26, 2020
    Date of Patent: January 5, 2021
    Assignee: Shenzhen Mindray Bio-Medical Electronics Co., Ltd.
    Inventors: Bo Ye, Guanzhen Wang, Jiantao Di, Ying Zhang, Huan Qi
  • Patent number: 10885646
    Abstract: A method of processing optical coherence tomography (OCT) scans, comprising: receiving OCT data comprising an OCT signal indicative of the level of scattering in a sample, the OCT data including the OCT signal for at least one scan through the sample, with the OCT signal having been measured at varying depth and position through the sample in each scan; processing the OCT data for each scan with depth to produce a indicative depth scan representative of the OCT signal at each depth through all of the scans; fitting a curve to the indicative depth scan, the curve comprising a first term which exponentially decays with respect to the depth and a second term which depends on the noise in the OCT signal; and calculating a compensated intensity for the OCT signal at each point through each scan, the compensated intensity comprising a ratio of a term comprising a logarithm of the OCT signal to a term comprising the logarithm of the fitted curve.
    Type: Grant
    Filed: April 20, 2017
    Date of Patent: January 5, 2021
    Assignee: Michelson Diagnostics Ltd.
    Inventor: Jonathan Denis Holmes
  • Patent number: 10881382
    Abstract: Disclosed herein is method and system for determining quality of semen sample. Trajectories of objects, identified in each of plurality of image frames of semen sample, are generated by tracking movement of the objects across image frames, and compensating a drift velocity of the semen sample. Further, generated trajectories are classified into sperm and non-sperm trajectories. Finally, total concentration estimate and total motility estimate of the semen sample are computed to generate a semen quality index, which indicates quality of the semen sample. In an embodiment, the method of present disclosure uses a multi-level Convolutional Neural Network (CNN) analysis technique for effectively classifying the object trajectories into sperm and non-sperm objects. Also, since the present method includes estimating and compensating drift velocity in the semen sample, it enhances overall accuracy of motility estimation and semen quality analysis.
    Type: Grant
    Filed: December 7, 2017
    Date of Patent: January 5, 2021
    Assignee: Sigtuple Technologies Private Limited
    Inventors: Karan Dewan, Bharath Cheluvaraju, Tathagato Rai Dastidar, Apurv Anand, Rohit Kumar Pandey
  • Patent number: 10843190
    Abstract: Apparatus and methods are described for use with a digital camera that is configured to acquire images of a bodily sample. Two or more stains are configured to stain the bodily sample. A computer processor drives the digital camera to acquire, for each of a plurality of imaging fields of the bodily sample, two or more digital images, at least one of the images being acquired under brightfield lighting conditions, and at least one of the images being acquired under fluorescent lighting conditions. The computer processor performs image processing on the digital images, by extracting visual classification features from the digital images and analyzing the extracted visual classification features. The computer processor outputs a result of the image processing that includes an indication of one or more entities that are contained within the sample. Other applications are also described.
    Type: Grant
    Filed: June 6, 2016
    Date of Patent: November 24, 2020
    Assignee: S.D. Sight Diagnostics Ltd.
    Inventors: Ido Bachelet, Joseph Joel Pollak, Daniel Levner, Yonatan Bilu, Noam Yorav-Raphael
  • Patent number: 10845310
    Abstract: A sensor device for quantifying luminescent targets configured in an at least one dimensional pattern. The sensor device comprises a detector for obtaining an at least one dimensional pattern of measured signals, wherein the detector is adapted for detecting the luminescence of the luminescent targets, resulting in a measured pattern. The sensor device moreover comprises a processor configured to correlate the measured pattern with at least one reference pattern, so as to generate a measurement signal representative for the quantification of luminescent targets. The at least one reference pattern is a recorded pattern or an expected pattern. A recorded pattern is a pattern which is obtained by the detector before the measured pattern is obtained.
    Type: Grant
    Filed: June 29, 2016
    Date of Patent: November 24, 2020
    Assignee: IMEC VZW
    Inventors: Peter Peumans, Liesbet Lagae, Willem Van Roy, Tim Stakenborg, Pol Van Dorpe
  • Patent number: 10845369
    Abstract: Sample-collecting devices, articles and methods of use are disclosed. Premoistened sample-collecting devices comprise 1,2-propanediol as a humectant, which promotes the retention of a liquid solution on the sample-collecting device during storage. 1,2-propanediol as humectant can be compatible with proteins and other reagents used to detect an analyte.
    Type: Grant
    Filed: March 1, 2016
    Date of Patent: November 24, 2020
    Assignee: 3M INNOVATIVE PROPERTIES COMPANY
    Inventors: Nerys Bennion, James Watkin, Russell M. I. Thomas, William J. Simpson
  • Patent number: 10842061
    Abstract: A soldering apparatus comprises a soldering mechanism and a management unit. The management unit converts operation history of the soldering mechanism into a numerical value, compares the numerical value to a predetermined threshold value, and generates a notifying signal if the numerical value exceeds the predetermined threshold value. The notifying signal may result in cessation of operation of the soldering mechanism. The notifying signal may result in a visual and/or audio reminder to the operator to inspect or replace a part of the soldering mechanism. With the management unit, the operator need not guess or rely on personal experience to determine when to perform an inspection or replacement. Reliance on personal experience can be error prone and lead to inefficiency. The management unit may reduce the possibility of soldering with a degraded soldering tip or other part, which may have an adverse effect on soldering quality.
    Type: Grant
    Filed: October 1, 2018
    Date of Patent: November 17, 2020
    Assignee: HAKKO CORPORATION
    Inventors: Yoshitomo Teraoka, Satoshi Manda
  • Patent number: 10829796
    Abstract: The present invention provides compositions, methods and kits for bacteria identification and anti-microbial susceptibility testing for the treatment of, for example, acute and chronic infections including infectious disease. The present invention is particularly useful in the identification of bacteria causing mastitis, and to antibiotic susceptibility testing to facilitate in the identification of an appropriate treatment for mastitis.
    Type: Grant
    Filed: February 26, 2016
    Date of Patent: November 10, 2020
    Assignee: MASTAPLEX LIMITED
    Inventor: Olaf Bork
  • Patent number: 10822582
    Abstract: A method for filtering a gas includes delivering a gas into a compartment of a gas filter assembly; applying a partial vacuum to the gas filter assembly so that the partial vacuum assists in drawing the gas through a porous filter body of the gas filter assembly that is at least partially disposed within the compartment of the gas filter assembly; and regulating the application of the partial vacuum based on a pressure reading of the gas upstream of the gas filter assembly.
    Type: Grant
    Filed: July 26, 2018
    Date of Patent: November 3, 2020
    Assignee: Life Technologies Corporation
    Inventors: Nephi D. Jones, Christopher D. Brau
  • Patent number: 10808275
    Abstract: Use of at least one chromogenic and/or fluorogenic phosphatase substrate for the detection and/or enumeration of enterobacteria in a sample likely to contain them, such as a food sample.
    Type: Grant
    Filed: April 1, 2014
    Date of Patent: October 20, 2020
    Assignee: BIOMÉRIEUX
    Inventors: Marie Cellier, Marie-Pierre Bourguignon, Diane Halimi, Sylvain Orenga
  • Patent number: 10809219
    Abstract: A sensor (100) for detecting a state of a fluid within a microfluidic plate (120) is disclosed. The sensor (100) comprises at least a first planar electrode (102) and a second planar electrode (104) arranged parallel to one another so as to form an electric capacitor (106). The first planar electrode (102) and the second planar electrode (104) are configured to generate an electric field (114) within the fluid, wherein the sensor (100) is configured to detect the state of the fluid by means of a capacitance of the electric capacitor (106). Further, a system (116) for controlling a preparation of a sample including a fluid is disclosed. Furthermore, a method for detecting a state of a fluid within a microfluidic plate (120) is disclosed.
    Type: Grant
    Filed: September 27, 2017
    Date of Patent: October 20, 2020
    Assignee: Roche Molecular Systems, Inc.
    Inventors: Claudio Cherubini, Michal Dobrzynski, Paul Federer, Thomas Kern, Lukas Schneider
  • Patent number: 10809677
    Abstract: A system, method, and computer readable medium are provided that monitors at least one of an input and output stream for at least one of a presence of a material of interest and a concentration of the material of interest; when the material of interest is present and/or has at least a threshold concentration, directs the at least one of an input and output stream to a waste location or path to discard the material of interest; and when the material of interest is either not present or does not have at least a threshold concentration, directs the at least one of an input and output stream to a byproduct location or path to recover the material of interest.
    Type: Grant
    Filed: June 12, 2015
    Date of Patent: October 20, 2020
    Assignee: ADA-ES, Inc.
    Inventor: Kenneth E. Baldrey
  • Patent number: 10774362
    Abstract: Sample preparation unit, preferably for sterility testing: a housing body including at least two ports adapted to serve as fluid inlet and/or fluid outlet, a membrane support, and a lid part such that a membrane chamber is defined adjacent said membrane support. One of at least two ports is arranged so as to allow a fluid transfer to/from a first volume of the membrane chamber at a position upstream of a membrane to be placed on the membrane support, and the other ports arranged to allow fluid transfer to/from a second volume of said membrane chamber at a position downstream of a membrane to be placed on said membrane support. A movable part is provided on housing body such that the movable part and housing body are movable relative to each other, selectively interrupting/establishing fluid transfer between at least one of two ports and the membrane chamber.
    Type: Grant
    Filed: December 4, 2014
    Date of Patent: September 15, 2020
    Assignee: Merck Patent GmbH
    Inventors: Anne Baumstummler, Marie Pressel, Sandra Laborde, David Lehmann, Gael Waiche
  • Patent number: 10774039
    Abstract: The invention relates to a polypeptide comprising an amino acid having a cyclopropene group wherein said cyclopropene group is joined to the amino acid via a carbamate group. Suitably the cyclopropene group is a 1,3-disubstituted cyclopropene such as a 1,3-dimethylcyclopropene. Suitably the cyclopropene group is present as a residue of a lysine amino acid. The invention also relates to methods of making the polypeptides. The invention also relates to an amino acid comprising cyclopropene wherein said cyclopropene group is joined to the amino acid moiety via a carbamate group.
    Type: Grant
    Filed: March 10, 2015
    Date of Patent: September 15, 2020
    Assignee: United Kingdom Research and Innovation
    Inventor: Thomas Elliott
  • Patent number: 10765069
    Abstract: Embodiments include a computer-implemented method, system, and computer program product for supplementing sub-optimal environmental conditions for plant growth, the embodiments include receiving plant data for a plant, and extracting optimal growth condition data from a database based at least in part on the plant. Embodiments also include obtaining sensor data from sensors monitoring current growth conditions of the plant and comparing the current growth conditions of the plant to the optimal growth condition data from the database to estimate a current cytoplasmic streaming rate. Embodiments include responsive to the comparison, calculating, by the calculation engine, a sound wave frequency to stimulate cytoplasmic streaming in the plant, transmitting the sound wave frequency to a speaker, and providing the sound wave frequency to the plant.
    Type: Grant
    Filed: May 17, 2018
    Date of Patent: September 8, 2020
    Assignee: INTERNATIONAL BUSINESS MACHINES CORPORATION
    Inventors: John S. Werner, Anuradha Rao, Pasquale A. Catalano, Arkadiy O. Tsfasman, Andrew G. Crimmins
  • Patent number: 10729325
    Abstract: The disclosure herein provides methods, systems, and devices for virtually staining biological tissue for enhanced visualization without use of an actual dye or tag by detecting how each pixel of an unstained tissue image changes in waveform after staining with a certain dye(s) and/or tag(s) or other transformation under a certain electromagnetic radiation source, developing a virtual staining transform based on such detection, and applying such virtual staining transform to an unstained biological tissue to virtually stain the tissue.
    Type: Grant
    Filed: March 25, 2014
    Date of Patent: August 4, 2020
    Assignee: Genetic Innovations, Inc.
    Inventors: Peter Bryant-Greenwood, Kevin P. Rosenblatt, Jeffrey N. Yu
  • Patent number: 10732186
    Abstract: The invention involves assays, diagnostics, kits, and assay components for determining levels of glycated CD59 in the assessment of gestational diabetes mellitus and/or related disorders and/or conditions.
    Type: Grant
    Filed: December 3, 2014
    Date of Patent: August 4, 2020
    Assignee: President and Fellows of Harvard College
    Inventors: Michael Chorev, Jose Alberto Halperin
  • Patent number: 10724095
    Abstract: The invention provides diagnostic markers of immunosenescence and methods of identifying individuals with impaired immune function based on the expression level of a combination of such markers in a biological sample obtained from said individual. Such combination of markers is useful for determining the susceptibility to nosocomial infections of an individual. Such combination of markers is also useful for predicting whether an individual will respond to active vaccination and become protected against recurring diseases.
    Type: Grant
    Filed: December 15, 2016
    Date of Patent: July 28, 2020
    Assignees: INSERM (INSTITUT NATIONAL DE LA SANTÉ ET DE LA RECHERCHE MÉDICALE), ASSISTANCE PUBLIQUE-HÔPITAUX DE PARIS (APHP), UNIVERSITÉ PARIS-EST CRÉTEIL VAL DE MARNE
    Inventors: Sophie Hue, Yves Levy
  • Patent number: 10704079
    Abstract: Presented herein are methods for the detection of specific beta-lactamases, including class A serine carbapenemases, metallo-beta-lactamases, AmpC beta-lactamases, and extended-spectrum beta-lactamases (ESBLs). The methods presented herein include methods that permit the detection of the presence of specific beta-lactamases in bacterial samples within as few as 2 to 10 minutes.
    Type: Grant
    Filed: February 26, 2018
    Date of Patent: July 7, 2020
    Assignee: Becton, Dickinson and Company
    Inventors: Mei Yang-Woytowitz, Charles Yu, Timothy Wiles
  • Patent number: 10697871
    Abstract: The present invention encompasses a micro-fluidic system having a closed-loop configuration in which inertial micro-fluidic separation of particles and/or cells is continuously repeated by feeding part of the output back to the input so that the purity and/or concentration of the particles and/or cell is maximized. The invention also includes methods of using the micro-fluidic system.
    Type: Grant
    Filed: October 6, 2017
    Date of Patent: June 30, 2020
    Assignee: Massachusetts Institute of Technology
    Inventors: Hyunryul Ryu, Kyungyong Choi, Jongyoon Han
  • Patent number: 10656143
    Abstract: The present disclosure relates to the field of medical technology, which provides methods and apparatuses for identifying red blood cells infected by plasmodium. The methods may include: obtaining a forward-scattered light signal, a side-scattered light signal and an optional fluorescence signal from cells in a blood sample; obtaining a first two-dimensional scattergram according to the forward-scattered light signal and the side-scattered light signal, or obtaining a three-dimensional scattergram according to the forward-scattered light signal, the side-scattered light signal and the fluorescence signal; and identifying cells located in a predetermined area of the first two-dimensional scattergram or the three-dimensional scattergram as the red blood cells infected by plasmodium. The apparatuses perform the methods. The methods and apparatuses can have better identification accuracy.
    Type: Grant
    Filed: October 31, 2012
    Date of Patent: May 19, 2020
    Assignee: SHENZHEN MINDRAY BIO-MEDICAL ELECTRONICS CO., LTD.
    Inventors: Bo Ye, Cheng Qian, Huan Qi