Fabric, Mat, Gauze, Or Fibrous Coating Patents (Class 435/399)
  • Patent number: 10080595
    Abstract: A device for bone fixation comprises an expansion part and a covering part. The expansion part has a fixing end and a top end and provides an expansion structure for being adjustable between a state of expansion and contraction. The covering part has a front end and a joining end, wherein the front end thereof is joined to the top end of the expansion part, and the joining end thereof is attached to the fixing end of the expansion part. The covering part is employed to cover the expansion structure. After the expansion part and the covering part are added to the bone, the covering part is unfurled to spread to the expansion state, and the medical filler is injected or pushed into the covering part.
    Type: Grant
    Filed: October 22, 2013
    Date of Patent: September 25, 2018
    Assignee: SPIRIT SPINE HOLDINGS CORPORATION, INC.
    Inventors: Shih-Hung Lin, Shih-Chun Lu, Kwan-Ku Lin, Shih-Hsiung Hsu
  • Patent number: 9427496
    Abstract: An artificial tissue including an internal mass transport network having a plurality of channels, wherein the channels are designed to substantially mimic naturally occurring vascular network and a method for creating an internal transport system within a tissue scaffold to improve circulation, diffusion, and mass transport properties by utilizing computer-aided tissue engineering (CATE). The artificial tissue has the internal mass transport network of channels embedded, deposited, or molded within a scaffold, wherein the channels are made from a biodegradable transporting material and the scaffold is made from a scaffold material. The artificial tissue of the invention includes a basic circulatory system embedded within the tissue scaffold. This system provides mass transport throughout the entire scaffold and degrades after the new circulatory system develops.
    Type: Grant
    Filed: February 21, 2006
    Date of Patent: August 30, 2016
    Assignee: Drexel University
    Inventors: Wei Sun, Jae Hyun Nam
  • Patent number: 9334473
    Abstract: Described herein is a three-dimensional cell culture scaffold composition comprising an absorbent rigid (AR) component, and in some embodiments, further comprises a gel component. The absorbent rigid component preferably comprises a glass fiber material. It is a surprising finding of the present invention that an AR component having a void volume of between approximately 70% and 95% results in a three-dimensional cell culture composition that allows for robust, high-throughput screening and high-content screening accessible tissue models with preserved cell morphology, heterogeneity of cell types and cell populations, extracellular matrix constituents, functional cell-cell and cell-extracellular matrix interactions and signaling with sufficient specificities to tissue physiology and pathology.
    Type: Grant
    Filed: August 8, 2013
    Date of Patent: May 10, 2016
    Inventor: Jelena Vukasinovic
  • Patent number: 9249383
    Abstract: The invention relates to an apparatus (1) for culturing anchorage-dependent cells. The apparatus (1) comprises a housing (2) with an inlet (4) and an outlet (5), and a plurality of culture plates (6) removably stacked within the housing (2). The housing (2) has a circumferential wall (7), a base (8) and a top wall (9). The base (8) comprises the inlet (4) and the top wall (9) comprises the outlet (5). The circumferential wall (7) of the housing (2) defines a longitudinal axis thereof, as well as an inner cross section perpendicular to the longitudinal axis. Shape and dimensions of the inner cross section are essentially uniform along the longitudinal axis. The culture plates (6) are arranged at least essentially parallel to each other. Each plate (6) is mounted and sealed to the circumferential wall (7) of the housing (2). Plates (6) are arranged at a distance from each other. Each culture plate (6) has a through hole (14), so that inlet (4) and outlet (5) are in fluid communication.
    Type: Grant
    Filed: October 8, 2009
    Date of Patent: February 2, 2016
    Assignee: AGENCY FOR SCIENCE TECHNOLOGY & RESEARCH
    Inventors: Hanry Yu, Lei Xia, Hwa Liang Leo
  • Publication number: 20150081012
    Abstract: A scaffold to form tissue membranes, comprising: at least one layer of mesh having a first side and a second side, the layer of mesh being either a woven wire metal mesh or a flat metal sheet that is acid-etched such that the layer of mesh includes a network of holes passing directly through the mesh from the first side to the second side; and at least one layer of cells at each side of the mesh enclosing the layer of mesh, such that the at least one layer of cells on the first side interacts with the at least one layer of cells on the second side through the network of holes to provide for structure integration.
    Type: Application
    Filed: November 24, 2014
    Publication date: March 19, 2015
    Inventors: Seyedhamed Alavi, Arash Kheradvar
  • Publication number: 20150024494
    Abstract: In one aspect, there is provided a cell culturing substrate including: a cell culture surface having a film attached thereto, wherein the film includes one or more plasma polymerized monomers; and a coating on the film-coated surface, the coating deposited from a coating solution comprising one or more extracellular matrix proteins and an aqueous solvent, where the total extracellular matrix protein concentration in the coating solution is about 1 ng/mL to about 1 mg/mL.
    Type: Application
    Filed: October 9, 2014
    Publication date: January 22, 2015
    Inventors: SUPARNA SANYAL, DEEPA SAXENA, SUSAN XIUQI QIAN, ELIZABETH ABRAHAM
  • Patent number: 8919281
    Abstract: A method is provided for culturing cochineal insects. In accordance with the method, a medium is created (101-109) from a mixture comprising a plant or cactus additive and a polymeric material. The medium is then inoculated (111) with a species selected from the group consisting of the genus Dactylopius.
    Type: Grant
    Filed: April 22, 2013
    Date of Patent: December 30, 2014
    Assignee: Badderloch Woad, Inc.
    Inventors: Constance M. Hendrickson, Denise Lynn Merkle
  • Patent number: 8871512
    Abstract: Sugar-acrylic monomers are synthesized to have a carbohydrate moiety linked to an acrylate group. The sugar-acrylic monomers may be polymerized to form polymers, adhesives, hydrogels, and the like. The sugar-acrylic monomers and polymers may be used in tissue engineering, adhesives and sealers, wound healing, and the like.
    Type: Grant
    Filed: October 27, 2010
    Date of Patent: October 28, 2014
    Assignee: Empire Technology Development LLC
    Inventors: William B. Carlson, Gregory D. Phelan, Phillip A. Sullivan
  • Publication number: 20140302606
    Abstract: The present invention aims to provide a method for producing auricular cartilage tissue having a sufficient thickness and mechanical strength, and auricular cartilage tissue produced by the method for producing auricular cartilage tissue. The present invention provides a method for producing auricular cartilage tissue, including the steps of: seeding auricular chondrocytes onto a non-woven fabric consisting of a bioabsorbable material having an average fiber diameter of 0.90 to 7.00 ?m; and forming a composite of the non-woven fabric seeded with the auricular chondrocytes and a mesh-like framework consisting of a non-bioabsorbable material, and shaping the composite.
    Type: Application
    Filed: November 30, 2012
    Publication date: October 9, 2014
    Inventors: Noritaka Isogai, Yoshihito Itani, Akeo Hagiwara, Shinichiro Morita, Kosuke Sawai, Koichi Hatakeyama
  • Patent number: 8778330
    Abstract: The present invention relates to methods of generating an ex vivo tissue-like system in a bioreactor system capable of supporting continuous production of, and output of cells and tissues and an ex vivo tissue system made therefrom.
    Type: Grant
    Filed: March 11, 2013
    Date of Patent: July 15, 2014
    Assignee: Aastrom Biosciences, Inc.
    Inventors: Lee Noll, Brian Hampson, Kristin Goltry, Samantha Snabes
  • Patent number: 8709809
    Abstract: A nanofiber is formed by combining one or more natural or synthetic polymeric materials and one or more than one cross-linking agents having at least two latent reactive activatable groups. The latent reactive activatable nanofiber may be used to modify the surface of a substrate by activating at least one of the latent reactive activatable groups to bond the nanofiber to the surface by the formation of a covalent bond between the surface of the substrate and the latent reactive activatable group. Some of the remaining latent reactive activatable group(s) are left accessible on the surface of the substrate, and may be used for further surface modification of the substrate. Biologically active materials may be immobilized on the nanofiber modified surface by reacting with the latent reactive groups that are accessible on the surface of the substrate.
    Type: Grant
    Filed: June 20, 2008
    Date of Patent: April 29, 2014
    Assignee: Innovative Surface Technologies, Inc.
    Inventors: Jie Wen, Patrick E. Guire
  • Patent number: 8669106
    Abstract: The invention provides, among other things, methods and systems for expanding CD133+ cells. The invention further provides methods and systems for increasing the blood flow to an ischemic tissue in a subject in need thereof, such as to ischemic myocardium. The invention further provides methods and systems for directing differentiation of expanded CD133+ cells. The invention further provides methods and systems for treating a subject with differentiated cells in a subject in need thereof.
    Type: Grant
    Filed: January 9, 2009
    Date of Patent: March 11, 2014
    Assignees: Arteriocyte Inc., Universite Pierre ET Marie Curie (Paris VI)
    Inventors: Ramasamy Sakthivel, Donald J. Brown, Hai-Quan Mao, Luc Douay, Vincent J. Pompili, Kevin McIntosh, Hiranmoy Das, Yukang Zhao
  • Patent number: 8628791
    Abstract: Silk is purified to eliminate immunogenic components (particularly sericin) and is used to form fabric that is used to form tissue-supporting prosthetic devices for implantation. The fabrics can carry functional groups, drugs, and other biological reagents. Applications include hernia repair, tissue wall reconstruction, and organ support, such as bladder slings. The silk fibers are arranged in parallel and, optionally, intertwined (e.g., twisted) to form a construct; sericin may be extracted at any point during the formation of the fabric, leaving a construct of silk fibroin fibers having excellent tensile strength and other mechanical properties.
    Type: Grant
    Filed: March 24, 2011
    Date of Patent: January 14, 2014
    Assignee: Allergan, Inc.
    Inventors: Gregory H. Altman, Jingson Chen, Rebecca L. Horan, David J. Horan
  • Publication number: 20140010850
    Abstract: Provided are method of generating a fiber from a globular protein such as albumin. Also provided are albumin fibers and fabrics and methods of using same for bonding a damaged tissue or for ex vivo or in vivo formation of a tissue.
    Type: Application
    Filed: September 16, 2013
    Publication date: January 9, 2014
    Applicant: Technion Research & Development Foundation Limited
    Inventors: Eyal ZUSSMAN, David Simhon, Shmuel Chervinsky, Abraham Katzir, Zvi Nevo, Yael Dror
  • Patent number: 8623398
    Abstract: Silk is purified to eliminate immunogenic components (particularly sericin) and is used to form fabric that is used to form tissue-supporting prosthetic devices for implantation. The fabrics can carry functional groups, drugs, and other biological reagents. Applications include hernia repair, tissue wall reconstruction, and organ support, such as bladder slings. The silk fibers are arranged in parallel and, optionally, intertwined (e.g., twisted) to form a construct; sericin may be extracted at any point during the formation of the fabric, leaving a construct of silk fibroin fibers having excellent tensile strength and other mechanical properties.
    Type: Grant
    Filed: July 30, 2012
    Date of Patent: January 7, 2014
    Assignee: Allergan, Inc.
    Inventors: Gregory H. Altman, Rebecca Horan, David J. Horan, Jingsong Chen
  • Patent number: 8586345
    Abstract: The invention is directed to formation and use of electroprocessed collagen, including use as an extracellular matrix and, together with cells, its use in forming engineered tissue. The engineered tissue can include the synthetic manufacture of specific organs or tissues which may be implanted into a recipient. The electroprocessed collagen may also be combined with other molecules in order to deliver substances to the site of application or implantation of the electroprocessed collagen. The collagen or collagen/cell suspension is electrodeposited onto a substrate to form tissues and organs.
    Type: Grant
    Filed: March 21, 2011
    Date of Patent: November 19, 2013
    Assignee: Virginia Commonwealth University Intellectual Property Foundation
    Inventors: David G. Simpson, Gary L. Bowlin, Gary E. Wnek, Peter J. Stevens, Marcus E. Carr, Jamil A. Matthews, Saravanamoorthy Rajendran
  • Publication number: 20130302896
    Abstract: Porous soy protein-based materials are provided. Also provided are tissue growth scaffolds comprising the porous soy protein-based materials. Methods for forming the porous soy protein-based materials and methods for growing tissue on the tissue growth scaffolds are also provided. The porous soy protein-based materials comprise a plurality of soy protein chains that are crosslinked in a three-dimensional structure that provides a high degree of porosity. In order to achieve highly porous structures, the soy proteins, which are globular in nature, can be partially denatured in order to facilitate crosslinking between, and entanglement of, the soy protein chains.
    Type: Application
    Filed: March 13, 2013
    Publication date: November 14, 2013
    Inventors: Ramille N. Shah, Karpagavalli Sundaresan, Karen B. Chien
  • Publication number: 20130209571
    Abstract: Provided herein is a method of making an aligned ECM scaffold useful in refractive correction of the eye and repair of the cornea. Methods of use of the scaffold as well as a scaffold construct are provided.
    Type: Application
    Filed: March 4, 2011
    Publication date: August 15, 2013
    Applicant: University of Pittsburgh- Of the Commonwealth System of Higher Education
    Inventors: Yiqin Du, James L. Funderburgh, William R. Wagner, Jian Wu
  • Publication number: 20130157367
    Abstract: The invention provides a method for producing nanofibers of an aliphatic polyester resin (a biodegradable resin) with higher productivity than heretofore achieved, for example, without the need of cumbersome steps such as drawing and with operability at normal temperature.
    Type: Application
    Filed: August 29, 2011
    Publication date: June 20, 2013
    Applicant: TMT Machinery, Inc.
    Inventors: Tsutomu Ono, Yukitaka Kimura
  • Publication number: 20130131830
    Abstract: The present invention includes an anisotropic scaffold, which is prepared by electrospinning a solution of matrix material upon a textile template. The present invention further includes a method of preparing such scaffold. The anisotropic scaffold of the invention finds use in tissue engineering and regenerative medicine.
    Type: Application
    Filed: May 23, 2011
    Publication date: May 23, 2013
    Applicant: Philadelphia University
    Inventors: Peter I. Lelkes, H. Gozde Senel, David Brookstein, Muthu Govindaraj
  • Publication number: 20130101548
    Abstract: A biodegradable hydrogel has been made based on high concentrations of whey protein isolate (WP1). WP1 gels of different compositions were fabricated by thermally inducing gelation of high-concentration suspensions of protein, and characterized for compressive strength and modulus, hydration swelling and drying properties, mechanical behavior change due to polysaccharide additives, and intrinsic pore network structure. The gels were shown to be compatible with bone cells and could be used as bone tissue scaffolds. In addition, WP1 fibers were produced by electrospinning. Several additives could be incorporated into the WPI gels, including structural additives, growth factors, amino acids, etc. The WP1 hydrogels can be made with glycerol to increase flexibility and stability.
    Type: Application
    Filed: April 1, 2011
    Publication date: April 25, 2013
    Inventors: James E. Henry, Mia Dvora
  • Patent number: 8304228
    Abstract: A manipulation tool is disclosed for producing cell material having multiple biological cells, which have a predefined geometrical arrangement. The tool includes a tool body, whose surface at least partially contacts the cell material, and a setting device for adjusting the tool body by a continuous expansion, so that geometrical properties of the surface change and an interior of the tool body is enlarged. The setting device is adapted to expand the tool body at an advance velocity in a range from 0.1 ?m/h to 1 mm/h.
    Type: Grant
    Filed: March 8, 2010
    Date of Patent: November 6, 2012
    Assignee: Fraunhofer-Gesellschaft zur Foerderung der Angewandten Forschung e.V.
    Inventors: Günter R. Fuhr, Heiko Zimmermann
  • Publication number: 20120264214
    Abstract: Sugar-acrylic monomers are synthesized to have a carbohydrate moiety linked to an acrylate group. The sugar-acrylic monomers may be polymerized to form polymers, adhesives, hydrogels, and the like. The sugar-acrylic monomers and polymers may be used in tissue engineering, adhesives and sealers, wound healing, and the like.
    Type: Application
    Filed: October 27, 2010
    Publication date: October 18, 2012
    Applicant: EMPIRE TECHNOLOGY DEVELOPMENT LLC
    Inventors: William B. Carlson, Gregory D. Phelan, Phillip A. Sullivan
  • Publication number: 20120225039
    Abstract: Cartilage has been constructed using biodegradable electrospun polymeric scaffolds seeded with chondrocytes or adult mesenchymal stem cells. More particularly engineered cartilage has been prepared where the cartilage has a biodegradable and biocompatible nanofibrous polymer support prepared by electrospinning and a plurality of chondocytes or mesenchymal stem cells dispersed in the pores of the support. The tissue engineered cartilages of the invention possess compressive strength properties similar to natural cartilage. Methods of preparing engineered tissues, including tissue engineered cartilages, are provided in which an electrospun nanofibrous polymer support is provided, the support is treated with a cell solution and the polymer-cell mixture cultured in a rotating bioreactor to generate the cartilage. The invention provides for the use of the tissue engineered cartilages in the treatment of cartilage degenerative diseases, reconstructive surgery, and cosmetic surgery.
    Type: Application
    Filed: May 15, 2012
    Publication date: September 6, 2012
    Applicant: The Government of the United States of America, as represented by the Secretary,Department of Health
    Inventors: Wan-Ju Li, Rocky S. Tuan
  • Patent number: 8183033
    Abstract: The invention relates to methods for preparing and performing quantitative PCR analyses, a new sealing device and a new use. According to the invention, a sample vessel containing the samples to be analyzed is sealed by placing a planar sealing device on the vessel to cover the samples and applying pressure on the sealing device in order to deform the sealing device so as to form a light-refracting geometry individually for the samples to be analyzed. The invention offers a convenient way of sealing the vessel and forming analysis-improving optical lenses over the samples simultaneously.
    Type: Grant
    Filed: March 21, 2008
    Date of Patent: May 22, 2012
    Assignee: Bioinnovations Oy
    Inventor: Bruce R Turner
  • Publication number: 20120122220
    Abstract: A method of preparing a stromal cell conditioned medium useful in expanding undifferentiated hemopoietic stem cells to increase the number of the hemopoietic stem cells is provided. The method comprising: (a) establishing a stromal cell culture in a stationary phase plug-flow bioreactor under continuous flow on a substrate in the form of a sheet, the substrate including a non-woven fibrous matrix forming a physiologically acceptable three-dimensional network of fibers, thereby expanding undifferentiated hemopoietic stem cells ; and (b) when a desired stromal cell density has been achieved, collecting medium from the stationary phase plug-flow bioreactor, thereby obtaining the stromal cell conditioned medium useful in expanding the undifferentiated hemopoietic stem cells.
    Type: Application
    Filed: January 27, 2012
    Publication date: May 17, 2012
    Inventors: Shoshana Merchav, Shai Meretski, Dov Zipori, Avinoam Kadouri
  • Patent number: 8163554
    Abstract: The present invention relates to an engineered biological material comprising or enriched for tissue of intervertebral disc; tissue derived from an engineered biological material; constructs comprising one or more tissues from an engineered biological material; methods for producing the engineered biological materials and constructs; and methods of using the engineered biological materials and constructs.
    Type: Grant
    Filed: September 20, 2007
    Date of Patent: April 24, 2012
    Assignee: Mount Sinai Hospital
    Inventor: Rita Kandel
  • Patent number: 8148142
    Abstract: A system and methods for processing organic waste material for residential type environments includes a reactor tank dimensioned to receive a predetermined amount of organic material therein. The amount of organic material is selected based upon the size of the residential service area the system is servicing. The system includes homogenizing the volume of organic waste materials and selectively introducing the homogenized organic material into a reaction tank. A system for selectively removing gaseous by-products from the anaerobic digestion of organic material within the reactor tank is provided, along with a system for selective removal of waste solids from the reactor tank to provide for substantially continuous processing of materials and production of usable gases.
    Type: Grant
    Filed: September 26, 2007
    Date of Patent: April 3, 2012
    Inventors: Michael R. Sapp, Rudy J. Wojtecki
  • Publication number: 20120064628
    Abstract: A scaffold for neurons consists of tubes sized to promote neural growth through the tubes. The tubes may be fixed to a substrate providing electrical or optical paths out from the interior of the tubes from sensors or stimulating probes at one or more locations along the length of the coaxial axons. Steering electrodes at spaces between tubes may be used to selectively promote the growth of interconnections of different axons in a one, two, or three-dimensional fashion.
    Type: Application
    Filed: September 13, 2010
    Publication date: March 15, 2012
    Inventors: Robert H. Blick, Justin Williams, Minrui Yu, Yu Huang
  • Publication number: 20120040462
    Abstract: Described herein are compositions for making nanofibers and nanofilms composed of metal oxides, organic polymers, or combinations thereof. Also described herein are methods for making nanofibers and nanofilms where the fibers are formed from a solution having more than one solvent, where the solvents are immiscible. Nanofibers and nanofilms composed of metal oxides, organic polymers or combinations thereof are described. Finally, methods for using the nanofibers and nanofilms are described.
    Type: Application
    Filed: October 26, 2011
    Publication date: February 16, 2012
    Inventors: Adra S. Baca, Marie D. Bryhan, Frederick E. Noll, Odessa N. Petzold, Michael W. Price, Wageesha Senaratne, Wanda J. Walczak
  • Patent number: 8110385
    Abstract: A linear biodevice is provided with fibers that have a curved portion in its cross section approximately perpendicular to the longitudinal direction and approximately spherical adhesive cells that adhere to the periphery of the fiber. A membrane-like biodevice is provided with a sheet that has an opening and approximately spherical adhesive cells that adhere to the opening. A curved portion is present in the cross section on an inner edge of the opening. A bioreactor is provided with a membrane-like biodevice in which spherical adhesive cells grow densely so as to close the openings of the sheet.
    Type: Grant
    Filed: June 30, 2005
    Date of Patent: February 7, 2012
    Assignee: Japan Agency for Marine-Earth Science and Technology
    Inventors: Satoshi Konishi, Tetsuya Miwa
  • Patent number: 8093038
    Abstract: An apparatus and method for coating micron-sized or sub-micron-sized particles such as living cells. The coating apparatus includes an encapsulation chamber enclosing a two-layer water-oil system for coating each islet cell with an aqueous polymeric coat. Islets together with an aqueous polymer solution are fed by a feed device that utilizes the principle of hydrodynamic focusing in order to ensure encapsulation of individual islets. The polymer in the aqueous coat is subsequently crosslinked by being exposed to laser light to produce structurally stable microcapsules of controllable thickness of the order of tens of microns. Encapsulated islets are removed from the encapsulation chamber by a valveless pump and recovered by filtration or centrifugation.
    Type: Grant
    Filed: September 17, 2007
    Date of Patent: January 10, 2012
    Assignee: Illinois Institute of Technology
    Inventor: Dimitri T. Hatziavramidis
  • Patent number: 8080419
    Abstract: This invention relates to methods for use in industrial production of proteins. Specifically, the present invention provides a method of recycling solid supports for cultivation of anchorage-dependent cells such as, e.g., microcarriers and Fibra-Cel® disks. Solid supports recycled by a method of the present invention allow obtaining a protein productivity level comparable to the productivity level obtained with non-recycled solid support. The method comprises the steps of rinsing with water, rinsing with a sodium hydroxide solution and second rinsing step with water.
    Type: Grant
    Filed: November 26, 2004
    Date of Patent: December 20, 2011
    Assignee: Ares Trading S.A.
    Inventors: Alain Bernard, Frederic Papp
  • Patent number: 8017395
    Abstract: The present invention generally relates to a method for seeding cells on to a support. In particular, the method relates to a method for seeding cells onto a porous hydrophobic support. The method utilizes centrifugal forces to uniformly guide cell seeding into the support with no loss in viability.
    Type: Grant
    Filed: December 16, 2005
    Date of Patent: September 13, 2011
    Assignee: Lifescan, Inc.
    Inventors: Alireza Rezania, Ragae Ghabrial
  • Publication number: 20110123596
    Abstract: The invention relates to a novel silica sol material containing at least one therapeutically active ingredient and its use for the production of bioabsorbable and biodegradable silica gel materials with improved properties. The materials, such as, for example, fibers, fleeces, powder, monolith and/or coating are employed, for example, in medical technology and/or human medicine, in particular for wound treatment.
    Type: Application
    Filed: July 3, 2009
    Publication date: May 26, 2011
    Applicant: BAYER INNOVATION GMBH
    Inventors: Iwer Baecker, Miranda Rothenburger Glaubitt, Joern Probst, Holger Egger
  • Publication number: 20110076771
    Abstract: The present disclosure relates to a fiber, a method of forming a fiber, a system for forming a fiber, and a method of engineering tissue from a fiber. The fiber includes an engineered geometric feature forming a non-Euclidian geometry.
    Type: Application
    Filed: September 23, 2010
    Publication date: March 31, 2011
    Applicants: ARMARK AUTHENTICATION TECHNOLOGIES, LLC, HILLS INC.
    Inventors: Peter D. GABRIELE, Jeffrey H. ROBERTSON, Jeffrey S. HAGGARD
  • Publication number: 20100297768
    Abstract: A nanofibrillar structure for cell culture and tissue engineering is disclosed. The nanofibrillar structure can be used in a variety of applications including methods for proliferating and/or differentiating cells and manufacturing a tissue. Also disclosed is an improved nanofiber comprising a lipid, lipophilic molecule, or chemically modified surface. The nanofibers can be used in a variety of applications including the formation of nanofibrillar structures for cell culture and tissue engineering.
    Type: Application
    Filed: April 26, 2010
    Publication date: November 25, 2010
    Applicant: Michigan State University
    Inventors: Melvin S. Schindler, Hoo Young Chung
  • Publication number: 20100291213
    Abstract: The disclosure relates to composite nonwoven fibrous web including an embedded phase having a population of particulates forming a substantially continuous three-dimensional network, and a matrix phase comprising a population of fibers forming a three-dimensional network around the particulates. The disclosure also relates to methods of making a composite nonwoven fibrous web including forming an embedded phase having a population of particulates in a substantially continuous three-dimensional network, and forming a matrix phase comprising a population of fibers forming a three-dimensional network around the particulates. Articles made from a composite nonwoven fibrous web prepared according to the methods as described above are also disclosed.
    Type: Application
    Filed: December 15, 2008
    Publication date: November 18, 2010
    Inventors: Michael R. Berrigan, Eric M. Moore
  • Patent number: 7799563
    Abstract: Methods and structures are disclosed where carrier fiber is used to enable the assembly of two and three dimensional structures of autologous tissue. Tissue is harvested from the donor, integrated with a carrier fiber, and assembled into complex forms rapidly. The structures can be tailored to the requirements of a specific medical procedure. The tissue is kept live and viable during extracorporeal assembly and the finished structure is emplaced in the donor's body. The use of a carrier fiber leader for pre-threading integration and assembly machines facilitates machine set up, drawing of the tissue into the process, and rapid integration and assembly of the multi-dimensional structures. Assembly can include providing tissue and fiber leaders extending from the structure for attaching the structure in place. The carrier fiber either is bio-absorbed as new tissue forms, or forms a bio-compatible substructure for the patient's native tissue.
    Type: Grant
    Filed: October 29, 2004
    Date of Patent: September 21, 2010
    Assignee: Warwick Mills, Inc.
    Inventors: Charles A. Howland, Virginia Houston-Howland, Jennifer K. White
  • Publication number: 20100183699
    Abstract: Novel compositions comprising genipin for cross-linking polymer fibers, are provided. In aspects of the invention the compositions further comprise a solvent system, wherein said solvent system comprises alcohol solvent and water. The genipin-based compositions are useful in methods for promoting the stabilization of fibers in an aqueous environment, and in tissue engineering. The novel genipin-based composition is also useful in methods of treating dermatological conditions.
    Type: Application
    Filed: January 21, 2010
    Publication date: July 22, 2010
    Inventors: Wankei Wan, Mina Mekhail
  • Patent number: 7704741
    Abstract: A method for producing cell material (20) having multiple biological cells (21), which have a predefined geometrical arrangement, includes the steps of providing a manipulation tool (10) having a tool body (11), whose surface (12, 14) at least partially contacts the cell material (20), and adjusting the manipulation tool (10) using a change of geometrical properties of the surface (12, 14) in such a way that the geometrical arrangement of the cells (21) is changed. A manipulation tool for performing a method of this type is also described.
    Type: Grant
    Filed: December 2, 2003
    Date of Patent: April 27, 2010
    Assignee: Fraunhofer-Gesellschaft zur Förderung der Angewandten Forschung e.V.
    Inventors: Gunter R. Fuhr, Heiko Zimmermann
  • Patent number: 7622299
    Abstract: In one aspect, the invention provides methods for forming a target tissue substitute. The methods of the invention comprise the following steps: (a) providing a scaffold comprising one or more layers of one or more arrays of microfibers, wherein one or more of the arrays of microfibers is designed to mimic the configuration of one or more structural elements in a target tissue; and (b) culturing cells on the scaffold to form a target tissue substitute. In another aspect, the invention provides implantable medical devices. The implantable medical devices of the invention comprise a scaffold comprising one or more layers of one or more arrays of microfibers, wherein one or more of the arrays of microfibers is arranged to mimic the configuration of one or more structural elements in a target tissue. Typically, cells are cultured on the scaffold to form a target tissue substitute.
    Type: Grant
    Filed: February 24, 2003
    Date of Patent: November 24, 2009
    Assignee: University of Washington
    Inventors: Joan E. Sanders, Thomas Neumann
  • Publication number: 20090017541
    Abstract: The present invention provides a bioreactor having a system that can grow cells, tissue, etc. while maintaining or improving their function, and finally recover the cells as they are with good efficiency. The bioreactor has a porous sheet-form material disposed in its main body, the porous sheet-form material being formed from a nonwoven fabric, etc. having high cell affinity in order to retain cells. This porous sheet-form material has a thermosensitive polymer and a cell-adhesive substance incorporated thereinto, and the porous sheet-form material is not only cell-adhesive but also allows cells and tissue to be detached from the porous sheet-form material as they are by, for example, cooling from 37° C. to 25° C. Furthermore, in order to efficiently ensure the bioactivity or the survival of the cells, it is arranged so that circulation of a culture medium in a culturing space of the bioreactor is of a radial flow type.
    Type: Application
    Filed: August 12, 2005
    Publication date: January 15, 2009
    Applicant: KYUSHU INSTITUTE OF TECHNOLOGY
    Inventor: Makoto Kodama
  • Patent number: 7445793
    Abstract: The present invention provides a scaffold in which cells can be stably retained and grafted in a uniform distribution state in the culture, preferable proliferation ability and viability can be secured, and particularly in the case of cartilage, fixation treatment such as suture can be carried out in the transplantation into affected parts after the culture, and the mechanical strength is provided sustainable for (weighted) compression at the initial stage of transplantation. The present invention relates to a 3-dimensional porous scaffold for tissue regeneration which comprises a structure composed of vertically long-shaped pores having a pore diameter of not less than 10 ?m to not more than 500 ?m and pore length of not less than 20 ?m to not more than 1 cm being juxtaposedly arranged obtained by a production process comprising rapid freeze-drying as a key technology.
    Type: Grant
    Filed: September 9, 2003
    Date of Patent: November 4, 2008
    Assignees: Kaneka Corporation, GC Corporation, Natl. Inst. of Adv. Ind. Science & Tech.
    Inventors: Hideo Niwa, Takeshi Fukuchi, Ichiro Shimizu, Masao Sato, Akiko Nishi, Kenji Yamashita, Tadashi Kaneko, Hajime Ohgushi, Koji Hattori, Kota Uematsu
  • Patent number: 7192769
    Abstract: The invention relates to a method for lymphoid tissue-specific cell production from hematopoietic progenitor cells in unique, three-dimensional culture devices, in the presence of antigen presenting cells and lymphoreticular stromal cells, and in the absence of exogenously added growth factors. The resulting lymphoid tissue-specific cells may be isolated at any sequential stage of differentiation and further expanded. The lymphoid tissue-specific cells also may be genetically altered at any stage of the process.
    Type: Grant
    Filed: May 31, 2002
    Date of Patent: March 20, 2007
    Assignee: Cytomatrix, LLC
    Inventors: Mark J. Pykett, Michael Rosenzweig, David T. Scadden, Mark C. Poznansky
  • Patent number: 7122371
    Abstract: An apparatus and method for a modular cell culture bioreactor comprises a plurality of chambers for cell culture; at least one reservoir containing a cell support medium; a plurality of conduits fluidly connecting the at least one reservoir with the plurality of chambers; and at least one pump fluidly connected through the plurality of conduits with the at least one reservoir and with the plurality of chambers to pump cell support medium therethrough; wherein each individual chamber of the plurality of chambers includes at least one three-dimensional matrix comprising polyethylene terephthalate, a plurality of channels carrying the cell support medium and having the matrix positioned in fluid communication therebetween, and at least two openings into each the channel, wherein a first the opening is in fluid connection with the pump and a second the opening is in fluid connection with the reservoir.
    Type: Grant
    Filed: March 30, 2005
    Date of Patent: October 17, 2006
    Assignee: The Florida State University Research Foundation, Inc.
    Inventor: Teng Ma
  • Patent number: 7087431
    Abstract: The present invention provides cultured leukemia cells. The method comprises isolating mononuclear cells, which contain leukemia cells, culturing the leukemia cells in a chamber having a scaffolding covered or surrounded with culture medium, where the scaffolding allows for leukemia cells to have cell to cell contacts in three dimensions. The subject leukemia cells are useful for screening compounds which inhibit or stimulate leukemia cell function or formation.
    Type: Grant
    Filed: March 1, 2001
    Date of Patent: August 8, 2006
    Assignee: University of Rochester
    Inventors: J. H. David Wu, Athanassios Mantalaris, Nicki Panoskaltsis
  • Patent number: 7015038
    Abstract: A method and an apparatus for preparing and culturing cells, particularly bone marrow stromal cells. The method includes the steps of placing an oxygenator and a scaffold in a container, such as a syringe, withdrawing bone marrow stromal cells with the syringe, evenly distributing the cells on the scaffold, connecting the syringe with a reservoir with a medium to enrich the syringe with the medium, and promoting the medium level movement in the syringe to culture bone marrow stromal cells.
    Type: Grant
    Filed: May 28, 2002
    Date of Patent: March 21, 2006
    Assignee: Industrial Technology Research Institute
    Inventors: King-Ming Chang, Ya-Chun Tseng, Long-Shuenn Jean, Benning Wang
  • Patent number: 7008634
    Abstract: Described are compositions with tethered growth effector molecules, and methods of using these compositions for growing cells and tissues. Growth effector molecules, including growth factors and extracellular matrix molecules, are flexibly tethered to a solid substrate. The compositions can be used either in vitro or in vivo to grow cells and tissues. By tethering the growth factors, they will not diffuse away from the desired location. By making the attachment flexible, the growth effector molecules can more naturally bind to cell surface receptors. A significant feature of these compositions and methods is that they enhance the biological response to the growth factors. The method also offers other advantages over the traditional methods, in which growth factors are delivered in soluble form: (1) the growth factor is localized to a desired target cell population; (2) significantly less growth factor is needed to exert a biologic response.
    Type: Grant
    Filed: March 3, 1995
    Date of Patent: March 7, 2006
    Assignee: Massachusetts Institute of Technology
    Inventors: Linda G. Cima, Edward W. Merrill, Philip R. Kuhl
  • Patent number: 6960427
    Abstract: The invention concerns a process for producing a three-dimensional bioartificial tissue having viable cells in or on a matrix, and by which cells and matrix can be cultivated into a tissue or a precursor of a tissue, a vascularized tissue of biological materials, obtained by this process, and an experimental reactor for scientific purposes and for producing clinically usable tissues and organs.
    Type: Grant
    Filed: February 28, 2002
    Date of Patent: November 1, 2005
    Assignee: Artiss GmbH
    Inventors: Axel Haverich, Theo Kofidis