Support Is A Fiber Patents (Class 435/398)
  • Patent number: 11111470
    Abstract: A packed-bed bioreactor system for culturing cells is provided, the system including a cell culture vessel having at least one interior reservoir, an inlet fluidly connected to the reservoir, and an outlet fluidly connected to the reservoir; and a cell culture matrix disposed in the reservoir. The cell culture matrix includes a structurally defined multi-layered substrate for adhering cells thereto, and each layer of the multi-layered substrate has a physical structure and a porosity that are substantially regular and uniform.
    Type: Grant
    Filed: September 30, 2020
    Date of Patent: September 7, 2021
    Assignee: Corning Incorporated
    Inventors: Ann MeeJin Ferrie, Vasiliy Nikolaevich Goral
  • Patent number: 10888638
    Abstract: The present invention relates to a method of producing a collagen membrane that has particular mechanical properties.
    Type: Grant
    Filed: May 15, 2019
    Date of Patent: January 12, 2021
    Inventor: Ming Hao Zheng
  • Patent number: 10449026
    Abstract: Aspects of the disclosure relate to synthetic tissue or organ scaffolds and methods and compositions for promoting or maintaining their structural integrity. Aspects of the disclosure are useful to prevent scaffold damage (e.g., delamination) during or after implantation into a host. Aspects of the disclosure are useful to stabilize tissue or organ scaffolds that include electrospun fibers.
    Type: Grant
    Filed: June 26, 2013
    Date of Patent: October 22, 2019
    Assignee: Biostage, Inc.
    Inventors: Ron Sostek, David Green, Linghui Meng, Sherif Soliman, Joseph Consiglio
  • Patent number: 10258644
    Abstract: A method for preventing and/or treating a disease associated with increased interleukin-1? and/or interleukin-6 and/or interleukin-8 activity and/or disease, in which a reduction in the activity of interleukin-1? and/or interleukin-6 and/or interleukin-8 is beneficial for healing includes utilizing a silicon-containing, biodegradable material containing a polyhydroxysilicic acid ethyl ester compound, with the proviso that wound defects including chronic diabetic-neuropathic ulcer, chronic leg ulcer, bedsores, secondary-healing infected wounds, non-irritating, primary-healing wounds, ablative lacerations and/or abrasions, are excluded from said disease that is prevented and/or treated with the silicon-containing, biodegradable material.
    Type: Grant
    Filed: May 10, 2016
    Date of Patent: April 16, 2019
    Assignee: SIANGSU SYNECOUN MEDICAL TECHNOLOGY CO., LTD.
    Inventors: Iwer Baecker, Christoph Suscheck
  • Patent number: 10208328
    Abstract: Provided are a rapid antimicrobial susceptibility test, based on an analysis of changes in morphology and growth pattern of a microbial cell under different concentrations of various antimicrobial agents, and an automated cell image analysis system therefor. The antimicrobial susceptibility test is rapidly performed based on an analysis of changes in morphology and growth pattern of a microbial cell under different concentrations of various antimicrobial agents, and this makes it possible to obtain highly reliable test results faster by six to seven times than the standard method recommended by Clinical and Laboratory Standards Institute (CLSI).
    Type: Grant
    Filed: October 14, 2015
    Date of Patent: February 19, 2019
    Assignee: QUANTAMATRIX INC.
    Inventors: Yong-Gyun Jung, Eun-Geun Kim, Jung Heon Yoo, Sunghoon Kwon, Jungil Choi, Hee Chan Kim, Jung Chan Lee, Eui Jong Kim, Sang Hoon Song, Sei Ick Joo, Ji Soo Lee
  • Patent number: 9724144
    Abstract: A bone regeneration membrane comprising: a dense layer made of resorbable polymer, said dense layer having first and second opposite surfaces and being adapted to form a barrier to cells and soft tissues, a nanofibrillar layer made of resorbable polymer and attached to the first surface of the dense layer, said nanofibrillar layer comprising fibers having a diameter of nanometer size, said fibers being interlaced so as to present an average pore size greater than 10 ?m to allow cell permeability and bone tissue regeneration, the nanofibrillar layer having a permeability ? between 0.4*10-9 m2 and 11*10-9 m2, preferably between 1*10-9 m2 and 4*10-9 m2, in particular substantially of 2*10-9 m2.
    Type: Grant
    Filed: July 8, 2011
    Date of Patent: August 8, 2017
    Assignees: UNIVERSITE DE NANTES, CHU NANTES
    Inventors: Alain Hoornaert, Pierre Layrolle, Jerome Sohier
  • Patent number: 9163331
    Abstract: The invention is directed to compositions and methods for preparing electrospun matrices comprising at least one natural biological material component and at least one synthetic polymer material. The natural component makes the matrices highly biocompatible while the molecular weight polymer component can impart additional strength mechanical strength to the scaffold and/or improve ease of manufacture by increasing viscosity and spinning characteristics of the solution during electrospining.
    Type: Grant
    Filed: November 18, 2009
    Date of Patent: October 20, 2015
    Assignee: Wake Forest University Health Sciences
    Inventors: Anthony Atala, James Yoo, Grace Lim, Richard Czerw, Shay Soker, Joel Stitzel
  • Patent number: 9029150
    Abstract: The present invention provides a cell culture substrate capable of culturing cells efficiently. The cell culture substrate of the present invention includes a substrate, a plurality of fibrous protrusions formed on the substrate, and water-repellent film formed on a surface of each of the fibrous protrusions. The fibrous protrusions are intertwined with each other to form a matrix structure. According to such a cell culture substrate, when a culture solution containing a specimen is discharged to the water-repellent fibrous protrusions, cells can be cultured easily without contact, thus enabling cells to be cultured efficiently.
    Type: Grant
    Filed: May 10, 2011
    Date of Patent: May 12, 2015
    Assignee: Panasonic Intellectual Property Management Co., Ltd.
    Inventors: Masaya Nakatani, Makoto Takahashi, Yoshiki Yamada, Takuya Oka
  • Patent number: 9029149
    Abstract: Provided herein are apparatus and systems for fabricating highly aligned arrays of polymeric fibers having isodiameters ranging from sub 50 nm to microns with lengths of several millimeters. The approach disclosed herein uses (e.g.) a micropipette to deliver polymeric solution which is collected in the form of aligned fibers on a rotating and linearly translating substrate. The methods deposit polymeric fibers on spherical surfaces and gapped surfaces with precise control, thus heralding new opportunities for a variety of applications employing polymeric fibers. The design workspace for depositing fibers disclosed herein is dependent upon processing parameters of rotational/linear translational speeds and material properties of solution rheologies. Techniques for fabrication of multilayer fiber arrays, for fabrication of cell growth scaffolds and for attachment of particles to the fiber arrays are also disclosed.
    Type: Grant
    Filed: July 30, 2009
    Date of Patent: May 12, 2015
    Assignee: Carnegie Mellon University
    Inventor: Amrinder Singh Nain
  • Publication number: 20150118747
    Abstract: An in vitro model system that guides the development of microvasculature, recapitulating the detailed organization of both its cellular and a-cellular components is established. Use of electrostretched fibrin microfibers enables both endothelial layer organization and co-culture of supporting perivascular (mural) cells such as vascular smooth muscle cells and pericytes. The fiber curvature affects the circumferential deposition of endothelial-produced ECM independently of cellular organization and induces deposition of higher quantities of vascular ECM proteins. Further, a luminal multicellular microvascular structure is disclosed.
    Type: Application
    Filed: October 31, 2014
    Publication date: April 30, 2015
    Inventors: Sharon Gerecht, Shuming Zhang, Sebastian F. Barreto Ortiz, Hai-Quan Mao
  • Publication number: 20150056703
    Abstract: A substrate for culturing cells that includes at least one fiber scaffold adapted to be contained within a disposable or non-disposable bioreactor, wherein the fiber scaffold further includes polymer fibers that have been created by electrospinning, and wherein the orientation of the fibers in the scaffold relative to one another is generally parallel, random, or both.
    Type: Application
    Filed: August 20, 2013
    Publication date: February 26, 2015
    Inventor: Jed Johnson
  • Patent number: 8911996
    Abstract: A porous scaffold is disclosed, the porous scaffold comprising electrospun polymeric nanofibers, wherein an average diameter of a pore of the porous scaffold is about 300 ?m is disclosed. An average diameter of the polymeric nanofibers ranges from about 100 to 400 nm. The scaffold may comprise a plurality of particles, the particles being greater than about 1 ?m in diameter. Methods of fabricating scaffolds, methods for generating tissue and methods of using scaffolds for tissue reconstruction are also disclosed.
    Type: Grant
    Filed: January 31, 2008
    Date of Patent: December 16, 2014
    Assignee: Technion Research & Development Foundation Limited
    Inventors: Samer Srouji, Eyal Zussman, Erella Livne
  • Publication number: 20140363890
    Abstract: Among others, the present invention provides devices for cell or tissue culture, comprising a three-dimensional structure, which further includes fibrils with beads and/or particles. The present invention also relates to novel methods for manufacturing devices for cell or tissue culture.
    Type: Application
    Filed: June 6, 2014
    Publication date: December 11, 2014
    Inventors: Iksoo Chun, Laura M. Frazier, Woraphon Kataphinan
  • Publication number: 20140341965
    Abstract: The invention relates to compositions comprising a monolayer of functional retinal pigment epithelial (RPE) cells attached to a transplantable, biodegradable scaffold. The invention also relates to methods of using these compositions.
    Type: Application
    Filed: March 14, 2014
    Publication date: November 20, 2014
    Applicant: Georgetown University
    Inventor: Nady Golestaneh
  • Patent number: 8889415
    Abstract: A method for expanding human corneal endothelial cells includes: (a) providing an amniotic membrane with or without amniotic cells, wherein the amniotic membrane has an extracellular matrix; (b) placing onto the amniotic membrane, a sheet of endothelial layer, or a cell suspension including human corneal endothelial stem cells; and (c) culturing the corneal endothelial cells on the amniotic membrane for a duration sufficient for the corneal endothelial stem cells to expand to an appropriate area. The invention also relates to a method for creating a surgical graft for a recipient site of a patient using the method for expanding human corneal endothelial cells, and the surgical graft prepared therefrom.
    Type: Grant
    Filed: April 30, 2007
    Date of Patent: November 18, 2014
    Inventor: Ray Jui-Fang Tsai
  • Publication number: 20140315302
    Abstract: The present invention relates to nanofibers. In particular, the present invention provides aligned nanofiber bundle assemblies. In some embodiments, the aligned nanofiber bundle assemblies are used for tissue regeneration, controlled growth of cells, and related methods (e.g., diagnostic methods, research methods, drug screening).
    Type: Application
    Filed: July 8, 2014
    Publication date: October 23, 2014
    Inventors: Samuel I. Stupp, Shuming Zhang, Alvaro Mata, Megan A. Greenfield
  • Publication number: 20140315235
    Abstract: The invention relates to a biocompatible scaffold for three dimensional cultivation of cells, said scaffold comprise one or more fibers randomly oriented to form a scaffold with open spaces for cultured cells. The one or more fibers are also coated with a bio-active coating and have a diameter of 100-3000 nm.
    Type: Application
    Filed: May 16, 2012
    Publication date: October 23, 2014
    Applicant: 3Dtro AB
    Inventors: Till Benjamin Puschmann, Milos Pekny, Carl Zanden, Johan Liu
  • Patent number: 8796023
    Abstract: A nanostructure composed of a plurality of peptides, each peptide containing at least one aromatic amino acid, whereby one or more of these peptides is end-capping modified, is disclosed. The nanostructure can take a tubular, fibrillar, planar or spherical shape, and can encapsulate, entrap or be coated by other materials. Methods of preparing the nanostructure, and devices and methods utilizing same are also disclosed.
    Type: Grant
    Filed: July 26, 2010
    Date of Patent: August 5, 2014
    Assignee: Ramot at Tel-Aviv University Ltd.
    Inventors: Meital Reches, Ehud Gazit
  • Publication number: 20140162365
    Abstract: A composite material sheet to support all or any part of the heart includes a lamina or a laminate of thin sheet made of a composite material which contains reinforcement fiber matrix. A value for the Young' modulus of the reinforcement fiber is as large as one hundred times or more of a value for the Young' modulus of the matrix.
    Type: Application
    Filed: March 4, 2013
    Publication date: June 12, 2014
    Inventor: Shoji YAMAMOTO
  • Patent number: 8747484
    Abstract: A method is disclosed for regenerating connective tissue by administering a scaffold comprising collagen fibers extracted from a soft coral. The length of the soft coral collagen fibers following stretching by about 15% is identical to the length of the fibers prior to stretching.
    Type: Grant
    Filed: July 18, 2013
    Date of Patent: June 10, 2014
    Assignees: Ramot at Tel-Aviv University Ltd., Technion Research & Development Foundation Limited
    Inventors: Yehuda Benayahu, Dafna Benayahu, Yoel Kashman, Amira Rudi, Yoram Lanir, Ido Sella, Einat Raz
  • Patent number: 8728817
    Abstract: The present invention provides methodologies and parameters for fabrication of the hybrid biomaterial by blending pure laminin or complex extracts of tissues containing laminin with biopolymers such as polycaprolactone (PCL), polylactic/polyglycolic acid copolymer (PLGA) or Polydioxanone (PDO) in fluoroalcohols (HFP, TFA), fabrication of substrates and scaffolds and devices from the hybrid biomaterial in forms such as films, nanofibers by electrospinning or microspheres, and the biological or biomedical use of the material or devices derived from it.
    Type: Grant
    Filed: March 10, 2011
    Date of Patent: May 20, 2014
    Assignee: University of Virginia Patent Foundation
    Inventors: Roy Clinton Ogle, Edward A. Botchwey, III, Rebekah A. Neal
  • Patent number: 8722850
    Abstract: There is described a group of novel self-assembling peptides (SAPs), comprising biotinylated and unbiotinylated sequences, hybrid peptide-peptoid sequences, branched sequences for a total of 48 tested motifs, showing a heterogeneous ensemble of spontaneously self-assembled structures at the nano- and microscale, ranging from short tabular fibers to twisted ribbons, nanotubes and hierarchical self-assembled micrometer-long sheets. Specifically, the SAPs according to the present invention which initially spontaneous assemble, surprisingly form stable solid scaffolds upon exposure to neutral pH buffer. Further these SAPs allow adhesion, proliferation and differentiation of murine and human neural stem cells and have self-healing propensity. They also did not exert toxic effects in the central nervous system, can stop bleeding and foster nervous regeneration.
    Type: Grant
    Filed: April 19, 2011
    Date of Patent: May 13, 2014
    Assignee: Universita' Degli Studi di Milano Bicocca
    Inventors: Angelo Luigi Vescovi, Fabrizio Gelain
  • Patent number: 8709809
    Abstract: A nanofiber is formed by combining one or more natural or synthetic polymeric materials and one or more than one cross-linking agents having at least two latent reactive activatable groups. The latent reactive activatable nanofiber may be used to modify the surface of a substrate by activating at least one of the latent reactive activatable groups to bond the nanofiber to the surface by the formation of a covalent bond between the surface of the substrate and the latent reactive activatable group. Some of the remaining latent reactive activatable group(s) are left accessible on the surface of the substrate, and may be used for further surface modification of the substrate. Biologically active materials may be immobilized on the nanofiber modified surface by reacting with the latent reactive groups that are accessible on the surface of the substrate.
    Type: Grant
    Filed: June 20, 2008
    Date of Patent: April 29, 2014
    Assignee: Innovative Surface Technologies, Inc.
    Inventors: Jie Wen, Patrick E. Guire
  • Publication number: 20140106356
    Abstract: The present invention provides a kit comprising a cell transfected with hepatic transcription regulators, and a culture medium that support growth of the cell. The present invention further provides a method for determining drug metabolism and predicting drug toxicity, comprising transfecting a cell with hepatic transcription regulators, and culturing the cell on a medium.
    Type: Application
    Filed: October 12, 2012
    Publication date: April 17, 2014
    Applicant: NATIONAL TAIWAN UNIVERSITY
    Inventors: Hsuan-Shu Lee, Guan-Tarn Huang, Kai-Chiang Yang, Tsai-Shin Chiang, Feng-Huei Lin
  • Patent number: 8669106
    Abstract: The invention provides, among other things, methods and systems for expanding CD133+ cells. The invention further provides methods and systems for increasing the blood flow to an ischemic tissue in a subject in need thereof, such as to ischemic myocardium. The invention further provides methods and systems for directing differentiation of expanded CD133+ cells. The invention further provides methods and systems for treating a subject with differentiated cells in a subject in need thereof.
    Type: Grant
    Filed: January 9, 2009
    Date of Patent: March 11, 2014
    Assignees: Arteriocyte Inc., Universite Pierre ET Marie Curie (Paris VI)
    Inventors: Ramasamy Sakthivel, Donald J. Brown, Hai-Quan Mao, Luc Douay, Vincent J. Pompili, Kevin McIntosh, Hiranmoy Das, Yukang Zhao
  • Publication number: 20140051169
    Abstract: A method for producing a nanofiber-based product includes providing a carrier material solution having a carrier material, and bringing the carrier material in contact with a collector by electrospinning. The carrier material essentially consists of a polymer being—at least after having contacted the collector—embedded in a polymer, which polymer is formed by a crosslinker of the general formula (I) wherein R1 is a single bond between the adjacent carbon atoms, or a carbohydrate chain having 1 to 10 carbon atoms and optionally bearing a hydroxy group, and wherein R2, R3, R4 and R5 are independently from each other a hydrogen; a carbohydrate chain having 1 to 10 carbon atoms and optionally bearing a hydroxy group; a hydroxy group; or a sulfhydryl group; with the provision that the compound bears at least two hydroxy groups, or two sulfhydryl groups, or one hydroxy group and one sulfhydryl group.
    Type: Application
    Filed: November 23, 2011
    Publication date: February 20, 2014
    Applicant: Spin Plant GmbH
    Inventors: Timothy Ganey, Jörg Meisel
  • Publication number: 20140010790
    Abstract: The present invention relates material based on microbial cellulose that is useful in culturing and transferring cells as well as delivering drugs. The material comprises cellulose nanofibers and/or derivatives thereof, based on microbial cellulose, wherein the cellulose nanofibers are in a form of a hydrogel or membrane. The invention also provides methods for producing these materials and compositions and uses thereof.
    Type: Application
    Filed: October 26, 2011
    Publication date: January 9, 2014
    Applicant: UPM-KYMMENE CORPORATION
    Inventors: Marjo Yliperttula, Patrick Laurén, Madhushree Bhattacharya, Yanru Lou, Antti Loukkanen
  • Patent number: 8617887
    Abstract: The present invention is generally in the field of neurological diseases and disorders, particular in the field of neurodegenerative diseases in which the myelin cover of nerves is lost. IL6R/IL6 chimera is used to promote the formation of oligodendrocytes from embryonic stem cells for treatment of neurodegenerative diseases or posttraumatic nerve damage.
    Type: Grant
    Filed: June 13, 2004
    Date of Patent: December 31, 2013
    Assignee: Yeda Research and Development Co. Ltd
    Inventors: Michel Revel, Peter Lonai, Rozemari Stirbu Lonai
  • Publication number: 20130344036
    Abstract: The present invention relates material that is useful in culturing and transferring cells as well as delivering cells. The material comprises plant derived cellulose nanofibers or derivatives thereof, wherein the cellulose nanofibers are in a form of a hydrogel or membrane. The invention also provides methods for producing these materials and compositions and uses thereof.
    Type: Application
    Filed: October 26, 2011
    Publication date: December 26, 2013
    Applicant: UPM-KYMMENE CORPORATION
    Inventors: Marjo Yliperttula, Patrick Laurén, Madhushree Bhattacharya, Yanru Lou, Antti Laukkanen
  • Publication number: 20130345140
    Abstract: The invention relates to cell support compositions comprising a basement membrane extract isolated from cardiac or smooth muscle. The invention also relates to methods of using the cell support compositions for supporting cellular functions.
    Type: Application
    Filed: August 25, 2011
    Publication date: December 26, 2013
    Applicant: LifeNet Health
    Inventor: Roy Ogle
  • Publication number: 20130323840
    Abstract: The present disclosure relates to a cylinder channel having a sawtooth-shaped cross section, a method for manufacturing same, a coaxial channel including same and a method for manufacturing a microfiber or a microparticle having a sawtooth-shaped cross section using same.
    Type: Application
    Filed: December 19, 2011
    Publication date: December 5, 2013
    Applicant: KOREA UNIVERSITY RESEARCH AND BUSINESS FOUNDATION
    Inventors: Sang Hoon Lee, Edward Kang
  • Patent number: 8597948
    Abstract: A medical device, said medical device, comprises: a first component having a non-biological material; a second component having a cloned biological material, said second component being attached to said first component, wherein said first component and said second component are operatively associated in a non-living medical device for at least one of treatment, diagnosis, cure, mitigation and prevention of disease, injury, handicap or condition in a living organism. In another aspect, a method comprises: preparing a cloned biological material from a tissue or an organ; attaching said biological material to a medical device; interfacing said biological material with the non-biological material; providing treatment, diagnosis, cure, mitigation and prevention of disease, injury, handicap or condition in a living organism.
    Type: Grant
    Filed: March 10, 2011
    Date of Patent: December 3, 2013
    Assignee: First Principles, Inc.
    Inventor: Keith A. Raniere
  • Publication number: 20130316376
    Abstract: A protein structure capable of selective interaction with an organic target is provided. The protein structure is a polymer comprising as a repeating structural unit a recombinant fusion protein that is capable of selective interaction with the organic target. The fusion protein is comprising the moieties B, REP and CT, and optionally NT. B is a non-spidroin moiety of more than 30 amino acid residues, which provides the capacity of selective interaction with the organic target. REP is a moiety of from 70 to 300 amino acid residues and is derived from the repetitive fragment of a spider silk protein. CT is a moiety of from 70 to 120 amino acid residues and is derived from the C-terminal fragment of a spider silk protein. NT is an optional moiety of from 100 to 160 amino acid residues and is derived from the N-terminal fragment of a spider silk protein. The fusion protein and protein structure thereof is useful as an affinity medium and a cell scaffold material.
    Type: Application
    Filed: October 25, 2011
    Publication date: November 28, 2013
    Applicant: SPIBER TECHNOLOGIES AB
    Inventors: My Hedhammar, Jan Johansson, Anna Rising, Per Åke Nygren
  • Patent number: 8586345
    Abstract: The invention is directed to formation and use of electroprocessed collagen, including use as an extracellular matrix and, together with cells, its use in forming engineered tissue. The engineered tissue can include the synthetic manufacture of specific organs or tissues which may be implanted into a recipient. The electroprocessed collagen may also be combined with other molecules in order to deliver substances to the site of application or implantation of the electroprocessed collagen. The collagen or collagen/cell suspension is electrodeposited onto a substrate to form tissues and organs.
    Type: Grant
    Filed: March 21, 2011
    Date of Patent: November 19, 2013
    Assignee: Virginia Commonwealth University Intellectual Property Foundation
    Inventors: David G. Simpson, Gary L. Bowlin, Gary E. Wnek, Peter J. Stevens, Marcus E. Carr, Jamil A. Matthews, Saravanamoorthy Rajendran
  • Publication number: 20130302895
    Abstract: An isolated collagen fiber is disclosed, wherein a length of the fiber prior to stretching by about 15%, is identical to a length of the fiber following said stretching by about 15%. The fiber comprises a Nuclear Magnetic Resonance (NMR) spectroscopic profile as shown in FIG. 1. Uses thereof and method of isolating are also disclosed.
    Type: Application
    Filed: July 18, 2013
    Publication date: November 14, 2013
    Inventors: Yehuda BENAYAHU, Dafna BENAYAHU, Yoel KASHMAN, Amira RUDI, Yoram LANIR, Ido SELLA, Einat RAZ
  • Publication number: 20130288287
    Abstract: A fibrous structure comprising an assembly of hair follicle cells within a fibrous matrix.
    Type: Application
    Filed: March 15, 2013
    Publication date: October 31, 2013
    Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH
    Inventors: Andrew Chwee Aun WAN, Tze Chiun Lim, Meng Fatt Leong, Jackie Y. Ying, Jerry Kah Chin TOH
  • Patent number: 8551778
    Abstract: The invention relates to a method for producing a preform by means of an electrospinning process. The present invention also relates to the use of the present preform as a substrate for growing human or animal tissue thereon. The present invention furthermore relates to a method for growing human or animal tissue on a substrate, wherein the present preform is used as the substrate.
    Type: Grant
    Filed: April 28, 2005
    Date of Patent: October 8, 2013
    Assignee: Technische Universiteit Eindhoven
    Inventor: Franciscus Petrus Thomas Baaijens
  • Patent number: 8546333
    Abstract: Provided are method of generating a fiber from a globular protein such as albumin. Also provided are albumin fibers and fabrics and methods of using same for bonding a damaged tissue or for ex vivo or in vivo formation of a tissue.
    Type: Grant
    Filed: January 31, 2008
    Date of Patent: October 1, 2013
    Assignee: Technion Research & Development Foundation Limited
    Inventors: Eyal Zussman, David Simhon, Shmuel Chervinsky, Abraham Katzir, Zvi Nevo, Yael Dror
  • Publication number: 20130245784
    Abstract: The present invention relates to a method for forming a tissue construct having a composite structure. The method includes providing a biodegradable substrate, wherein the substrate is adapted to allow deposition or growth of a plurality of cells; providing a vascularized layer comprising a plurality of blood vessels therein; and adhering the vascularized layer to the substrate.
    Type: Application
    Filed: September 22, 2011
    Publication date: September 19, 2013
    Applicant: NANYANG TECHNOLOGICAL UNIVERSITY
    Inventors: Lay Poh Tan, Philip Wong, Yin Chiang Freddy Boey, Subbu Venkatraman
  • Patent number: 8486699
    Abstract: Two cell lines, PICM-19H and PICM-19B, were derived from the bipotent ARS-PICM-19 pig liver stem cell line. The unipotent porcine stem cell line PICM-19H differentiates exclusively into hepatocytes and can be induced to express CYP450 enzymes. The growth rate and cell density in culture, morphological features, and hepatocyte detoxification functions, i.e., inducible CYP450 activity, ammonia clearance, and urea production of the PICM-19H cells were evaluated for their application in artificial liver devices. PICM-19H cells contain numerous mitochondria, Golgi apparatus, smooth and rough endoplasmic reticulum, vesicular bodies and occasional lipid vacuoles and display inducible CYP450 activity, clear ammonia, and produce urea in a glutamine-free medium. The data indicate that both cell lines, either together or alone, may be useful as the cellular substrate for an artificial liver device. The results demonstrate the potential for the use of PICM-19H cells in drug biotransformation and toxicity testing.
    Type: Grant
    Filed: May 23, 2008
    Date of Patent: July 16, 2013
    Assignee: The United States of America as Represented by the Secretary of Agriculture
    Inventors: Neil C. Talbot, Thomas J. Caperna, Ryan Willard
  • Publication number: 20130177623
    Abstract: Activated platelet-rich plasma (aPRP) is electrospun into fibrous matrices which are used to deliver components of aPRP to a site of action in a sustained manner. The electrospun matrices are used, for example, for tissue engineering applications and for the treatment of wounds.
    Type: Application
    Filed: September 21, 2011
    Publication date: July 11, 2013
    Inventors: Gary L. Bowlin, Patricia S. Wolfe, Scott A. Sell
  • Publication number: 20130133980
    Abstract: This disclosure relates to articles that comprise polymeric winged fibers. The winged fibers have a high surface area because of their structure, which includes a core surrounded by a plurality of lobes. Channels of one micron or less in width are formed between adjacent lobes to form paths for the capture and/or transport of gases, liquids or particles. The winged fibers are assembled in woven or non-woven fabrics for use in wipes, absorbent pads, composite structures, apparel, outdoor wear, bedding, filtration systems, purification/separation systems, thermal and acoustic insulation, cell scaffolding, and battery membranes.
    Type: Application
    Filed: January 22, 2013
    Publication date: May 30, 2013
    Applicant: Allasso Industries, Inc.
    Inventor: Allasso Industries, Inc.
  • Publication number: 20130115698
    Abstract: A method and a combination for the cultivation of eukaryotic cells are provided, as well as a method for preparation of eukaryotic cells. The methods comprise providing a sample of eukaryotic cells to be cultured, applying said sample to a cell scaffold material; and maintaining said cell scaffold material having cells applied thereto under conditions suitable for cell culture. The combination comprises eukaryotic cells and a cell scaffold material. The cell scaffold material comprises a polymer of a spider silk protein.
    Type: Application
    Filed: April 12, 2011
    Publication date: May 9, 2013
    Applicant: SPIBER TECHNOLOGIES AB
    Inventors: Jan Johansson, Anna Rising, My Hedhammar, Ulrika Johansson, Mona Widhe
  • Publication number: 20130105348
    Abstract: The invention relates to implantable collagen devices made by seeding at least one elongate collagen construct, e.g., comprising at least one elongate synthetic collagen fiber with a plurality of cells and applying a strain and/or stress to the at least one elongate collagen fiber to induce the cells to differentiate into target phenotypes, e.g., tendon or ligament phenotype cells (and/or fibroblasts), typically with an extracellular matrix of collagen to organize into a tissue on the at least one collagen fiber.
    Type: Application
    Filed: November 2, 2012
    Publication date: May 2, 2013
    Inventor: MiMedx Group, Inc.
  • Publication number: 20130053958
    Abstract: Prosthetic ligaments and tendons comprising ligament- or tendon-mimicking nanofibers and methods of making such nanofibers and prosthetic ligaments and tendons.
    Type: Application
    Filed: March 27, 2012
    Publication date: February 28, 2013
    Inventor: Javier Macossay-Torres
  • Publication number: 20130052712
    Abstract: The present invention relates to technology of immobilizing or coating various functional bioactive substances on various surfaces without physical chemical treatment using mussel adhesive protein. More specifically, the present invention relates to a functional scaffold for tissue engineering comprising artificial extracellular matrix, manufactured by coating various functional bioactive substances on the surface of nanofiber and metal scaffold using mussel adhesive protein, and a method of manufacturing the same.
    Type: Application
    Filed: March 26, 2012
    Publication date: February 28, 2013
    Applicant: POSTECH ACADEMY-INDUSTRY FOUNDATION
    Inventors: Hyung Joon Cha, Bum Jin Kim, Yoo Seong Choi, Bong-Hyuk Choi
  • Publication number: 20130052254
    Abstract: Provided is an electroactive structure and method for growing isolated differentiable cells comprising a three dimensional matrix of fibers formed of a biocompatible synthetic piezoelectric polymeric material, wherein the matrix of fibers is seeded with the isolated differentiable cells and forms a supporting scaffold for growing the isolated differentiable cells, and wherein the matrix of fibers stimulates differentiation of the isolated differentiable cells into a mature cell phenotype on the structure.
    Type: Application
    Filed: October 12, 2012
    Publication date: February 28, 2013
    Applicant: NEW JERSEY INSTITUTE OF TECHNOLOGY
    Inventor: NEW JERSEY INSTITUTE OF TECHNOLOGY
  • Patent number: 8383408
    Abstract: A nanofibrillar structure for cell culture and tissue engineering is disclosed. The nanofibrillar structure can be used in a variety of applications including methods for proliferating and/or differentiating cells and manufacturing a tissue. Also disclosed is an improved nanofiber comprising a lipid, lipophilic molecule, or chemically modified surface. The nanofibers can be used in a variety of applications including the formation of nanofibrillar structures for cell culture and tissue engineering.
    Type: Grant
    Filed: April 26, 2010
    Date of Patent: February 26, 2013
    Assignee: Board of Trustees of Michigan State University
    Inventors: Melvin S. Schindler, Hoo Young Chung
  • Publication number: 20130045536
    Abstract: The present invention provides a cell culture substrate capable of culturing cells efficiently. The cell culture substrate of the present invention includes a substrate, a plurality of fibrous protrusions formed on the substrate, and water-repellent film formed on a surface of each of the fibrous protrusions. The fibrous protrusions are intertwined with each other to form a matrix structure. According to such a cell culture substrate, when a culture solution containing a specimen is discharged to the water-repellent fibrous protrusions, cells can be cultured easily without contact, thus enabling cells to be cultured efficiently.
    Type: Application
    Filed: May 10, 2011
    Publication date: February 21, 2013
    Applicant: PANASONIC CORPORATION
    Inventors: Masaya Nakatani, Makoto Takahashi, Yoshiki Yamada, Takuya Oka
  • Publication number: 20130023050
    Abstract: The invention provides a method of culturing a corneal endothelial cell by use of a culture medium containing an ascorbic acid derivative and the like.
    Type: Application
    Filed: February 4, 2011
    Publication date: January 24, 2013
    Applicant: FOUNDATION FOR BIOMEDICAL RESEARCH AND INNOVATION
    Inventors: Nobuyuki Shima, Miwa Kimoto, Masahiro Yamaguchi, Satoru Yamagami