Using Multifield Media Patents (Class 435/40)
  • Patent number: 9616424
    Abstract: Provided is a microfluidic apparatus including: a microfluidic structure for providing spaces for receiving a fluid and for forming channels, through which the fluid flows; and valves for controlling the flow of fluid through the channels in the microfluidic apparatus. The microfluidic structure includes: a sample chamber; a sample separation unit receiving the sample from the sample chamber and separating a supernatant from the sample by using a centrifugal force; a testing unit receiving the supernatant from the sample separation unit for detecting a specimen from the supernatant using an antigen-antibody reaction, and a quality control chamber for identifying reliability of the test.
    Type: Grant
    Filed: December 28, 2012
    Date of Patent: April 11, 2017
    Assignee: SAMSUNG ELECTRONICS CO., LTD.
    Inventors: Beom Seok Lee, Ji Won Kim, Jeong Gun Lee, Kui Hyun Kim
  • Patent number: 8815574
    Abstract: A system and method is provided for non-invasively measuring changes in a specimen suspected of containing one or more microbes, by monitoring changes in the dielectric constant of the specimen caused by metabolic processes of such microbes.
    Type: Grant
    Filed: December 22, 2005
    Date of Patent: August 26, 2014
    Assignee: Becton, Dickinson and Company
    Inventors: Nicholas R. Bachur, Jr., John W. Neubert
  • Patent number: 8557539
    Abstract: A new device and method for detecting the presence of living microorganisms in test samples are described. The device comprises a container with at least one section transparent to light, a growth zone located in said container containing a mixture of growth media capable of supporting growth of the microorganisms, and at least one indicator substrate that changes its optical properties due to growth of the microorganisms. A detection zone is located in the container adjacent to the transparent section, and a barrier layer comprising porous solid material separates the two zones, allowing diffusion of molecules and ions of metabolic by-products of the organisms, while preventing microorganisms and particulate matter of the test sample from penetrating into the detection zone.
    Type: Grant
    Filed: June 21, 2007
    Date of Patent: October 15, 2013
    Assignee: BioLumix Inc.
    Inventors: Gideon Eden, Ruth Eden
  • Patent number: 8551731
    Abstract: A stabilized hematoxylin composition is disclosed that includes one or both of a host compound and an antioxidant. The disclosed composition exhibits sufficient stability to be utilized in an automated staining process without undue degradation prior to use of the composition to stain a biological sample. Methods of using and making the stabilized composition also are disclosed.
    Type: Grant
    Filed: June 22, 2012
    Date of Patent: October 8, 2013
    Assignee: Ventana Medical Systems, Inc.
    Inventors: Jerome W. Kosmeder, Christopher Bieniarz, Penny Towne, Linda Willoughby Kivi
  • Patent number: 8389233
    Abstract: Provided are methods for sampling, testing and validating test lots, comprising: assembling a plurality of product portions from each of a plurality of test lots and combining the portions to provide a corresponding set of test lot samples; enriching the test lot samples; removing portions of each enriched sample, and combining the removed portions to provide a modular composite sample; and testing of the modular composite sample, and individual testing of the enriched test lot samples, using at least one suitable detection assay for a target microbe or organism, wherein when such testing is negative all test lots are validated, and wherein when such testing is positive with respect to the modular composite sample, or with respect to an individual enriched test lot sample, individual test lots may nonetheless yet be validated by further testing of a portion of respective initially-negative enriched test lot samples and obtaining negative results.
    Type: Grant
    Filed: January 27, 2009
    Date of Patent: March 5, 2013
    Assignee: Institute for Environmental Health, Inc.
    Inventor: Mansour Samadpour
  • Patent number: 8273312
    Abstract: A liquid testing assembly for testing a liquid, the assembly comprising a test vessel and a stopper adapted to fit into a free end of the vessel. The stopper substantially hermetically seals the test vessel from the ambient. Further, the assembly includes a support coated with one or more identifying materials for identifying one or more constituents of the liquid. The support is fixed in the stopper and/or the vessel and extends into its interior for a predetermined distance. The liquid testing assembly when assembled is pre-evacuated to a predetermined vacuum sufficient to draw a predetermined volume of liquid to be sampled into the test vessel. The predetermined volume is of such an amount that it wets the one or more identifying materials ensuring identification of one or more constituents present in the liquid. A kit employing the liquid testing assembly is also discussed.
    Type: Grant
    Filed: January 22, 2008
    Date of Patent: September 25, 2012
    Assignee: Association for Public Health
    Inventors: Gadi Porat, Joel Stern, Boaz Rimon, Yoram Cohen
  • Patent number: 8133356
    Abstract: A method for monitoring one or more deposits including microbiological deposits on a surface immersed in an aqueous medium of a process is disclosed. The method comprises: (a) providing a QCM that is in contact with an aqueous medium, wherein said QCM has a top side surface contacting the aqueous medium, and a second, bottom side surface isolated from the aqueous medium; (b) providing a composition that is selective for one or more deposit types, including microbiological deposits without the use of an antigen or an antibody; (c) applying said composition to the top side of said QCM surface; (d) measuring the rate of deposition of said deposits from the aqueous medium onto the top side of said QCM surface; and (e) optionally taking corrective action to change one or more process parameters, or chemistry applied to the process, or a combination thereof, to achieve a desired result in regards to microbiological deposition.
    Type: Grant
    Filed: June 19, 2008
    Date of Patent: March 13, 2012
    Assignee: Nalco Company
    Inventors: Sergey M. Shevchenko, Michael J Murcia, Patrick J. Macuch
  • Patent number: 8119363
    Abstract: An apparatus and method for automatic thin-layer cell sample slide preparation without requiring human intervention are disclosed. According to one embodiment, a cell sample is measured to derive a cell sample measurement. An estimation of total cellularity of the cell sample is determined based upon the cell sample measurement. A differential volume of diluent is dispensed to the cell sample based upon the estimation of total cellularity of the cell sample to form a cell suspension. In one embodiment, a differential volume of the cell suspension and a differential volume of cell adherent may be combined based upon the estimation of total cellularity of the cell sample to form a cell mixture. A differential volume of the cell suspension or cell mixture (if an adherent is mixed with the cell suspension) is dispensed onto a surface of a sample slide.
    Type: Grant
    Filed: May 2, 2008
    Date of Patent: February 21, 2012
    Assignee: CellSolutions, LLC
    Inventors: Ernest A. Knesel, Bradley W. Knesel, Joel C. Dry, Kevin J. Rackers, Robert M. Hicks, Jr., Benjamin A. Perrot
  • Patent number: 8026080
    Abstract: The present invention includes devices, systems, and methods for assaying cells using cell-substrate impedance monitoring. In one aspect, the invention provides cell-substrate impedance monitoring devices that comprise electrode arrays on a nonconducting substrate, in which each of the arrays has an approximately uniform electrode resistance across the entire array. In another aspect, the invention provides cell-substrate monitoring systems comprising one or more cell-substrate monitoring devices comprising multiple wells each having an electrode array, an impedance analyzer, a device station that connects arrays of individual wells to the impedance analyzer, and software for controlling the device station and impedance analyzer. In another aspect, the invention provides cellular assays that use impedance monitoring to detect changes in cell behavior or state. In some preferred aspects, the assays are designed to investigate the affects of compounds on cells, such as cytotoxicity assays.
    Type: Grant
    Filed: March 15, 2007
    Date of Patent: September 27, 2011
    Assignee: Acea Biosciences, Inc.
    Inventors: Xiaobo Wang, Yama A. Abassi, Xiao Xu, Jiangbo Gan
  • Patent number: 7915006
    Abstract: Apparatus, methods, and systems for high throughput, useful sampling of seed, wherein viability is optionally maintained, are disclosed. Seed from one generation in a plant advancement experiment is individually sampled by removal and collection of tissue from the seed. The tissue is then processed to derive one or more biochemical, genetic, or phenotypic characteristic of the seed before a decision is made whether to utilize that seed further in a plant advancement experiment or other plant research and development. In some embodiments of the method, the sampling is controlled to remove a useful amount of tissue for analytical purposes without significant effect on viability potential of the sampled seed. In some embodiments, the sampling is controlled to deter contamination of the sample. In some embodiments, the seed is automatically positioned and oriented to facilitate efficient and accurate sampling.
    Type: Grant
    Filed: November 13, 2007
    Date of Patent: March 29, 2011
    Assignee: Pioneer Hi-Bred International, Inc.
    Inventors: Jason Cope, David Kurth
  • Publication number: 20110039298
    Abstract: A blood test instrument using a disposable cartridge and a method of measuring a blood sample using the instrument are disclosed. The instrument includes a cell counting station for counting blood cells by electrical resistance measurement, a pressure actuating component adapted to apply a pressure alternately on two flexible receptacles of a disposable cartridge removably placed in the instrument to cause flowing of a mixture of a blood sample and a liquid agent between the two receptacles to obtain proper mixing, and a conduit adapted to deliver the mixture to the cell counting station for counting. After measuring the blood sample, the instrument withdraws a washing liquid contained in another receptacle of the disposable cartridge and uses the washing liquid to clean the instrument and to deliver the mixture back to the cartridge for disposal.
    Type: Application
    Filed: October 22, 2010
    Publication date: February 17, 2011
    Inventors: Ingemar BERNDTSSON, Lars Svensson, Lennart Niklason
  • Publication number: 20100261226
    Abstract: Bioreactors suitable for housing a predetermined volume of culture medium comprising a plurality of containers of approximately equal internal volume in which the culture medium resides, wherein the predetermined volume of culture medium is retained in approximately equal amounts within each of the plurality of containers during operation of the bioreactor, and wherein the internal volume of each container is selected so that the amount of culture medium in each container exceeds 50 vol. % of the container internal volume during operation of the bioreactor, and related methods of use.
    Type: Application
    Filed: April 14, 2009
    Publication date: October 14, 2010
    Inventor: Sarfaraz K. Niazi
  • Patent number: 7771660
    Abstract: Methods for identifying a biological particle in a sample medium include generating an Electrophoretic Quasi-elastic Light Scattering (EQELS) spectrum for the biological particle in the sample medium. The EQELS spectrum is compared to a reference database comprising a plurality of spectra, and each of the plurality of spectra correspond to an EQELS spectrum for one of a plurality of known biological particles. The biological particle in the sample medium is identified from the comparison.
    Type: Grant
    Filed: May 3, 2005
    Date of Patent: August 10, 2010
    Assignee: University of North Carolina at Chapel Hill
    Inventor: Don A. Gabriel
  • Patent number: 7744854
    Abstract: This invention provides a method for a method for optimizing the regimen for the administration of chemotherapeutic agents. The method comprises administration of a selenium compound to an individual, monitoring the modulation of tumor vessel maturation (TVM) to identify an optimal time for administration of a chemotherapeutic agent. This invention also provides a method to determine whether or not a tumor is likely to be a responder to chemotherapy. The method comprises administration of a selenium compound to an individual and determining whether or not an increase in TVM is observed. An increased TVM following administration of selenium is an indication that the tumor will likely respond the chemotherapy.
    Type: Grant
    Filed: March 23, 2007
    Date of Patent: June 29, 2010
    Assignee: Health Research, Inc.
    Inventors: Youcef M. Rustum, Arup Bhattacharya, Karoly Toth, Harry K. Slocum, Rami G. Azrak, Sreenivasulu Chintala, Mukund Seshadri, Richard Mazurchuk
  • Patent number: 7745169
    Abstract: A device and method simultaneously detects and enumerates two groups of microorganisms in a test sample, utilizing a single test container. In the container liquid growth media, a chromogenic substrate and a fluorogenic substrate are mixed with the test sample. The test container is incubated to allow bacterial growth and metabolism. Spectral changes of the substrates are dynamically detected using two external light sources aimed at a transparent section of the test container, and a single external photo detector. One light source operates in the visible band and the second in the long ultraviolet band. The two dynamic time patterns generated by the two substrates are analyzed in real time to determine the presence or absence of each microorganisms group and to enumerate their original concentrations in the test sample.
    Type: Grant
    Filed: June 21, 2007
    Date of Patent: June 29, 2010
    Assignee: BioLumix Inc.
    Inventors: Gideon Eden, Ruth Eden
  • Patent number: 7642069
    Abstract: An improved process for the production of streptokinase using a genetically engineered strain of Escherichia coli which overproduces streptokinase intracellularly and more particularly, the overall process disclosed herein, concerns with an improvement in the fermentative production of streptokinase using an optimized growth medium mainly comprised of simple salts and trace-elements; thus, in principal, the present process constitutes an improved and more economical means for the production of streptokinase which may be useful in thrombolytic therapy.
    Type: Grant
    Filed: December 9, 2005
    Date of Patent: January 5, 2010
    Assignee: Council of Scientific & Industrial Research
    Inventors: Vinay Venkatrao Vyas, Govindan Rajamohan, Ramandeep, Kanak Lata Dikshit
  • Patent number: 7582472
    Abstract: There is provided a device for partitioning a liquefied sample into discrete volumes. The device includes a bottom member; a top member disposed adjacent the bottom member; and at least one channel member disposed between the top and bottom members. The at least one channel member is at least partially defined by the top and bottom members and has first and second end portions. The first end portion of the at least one channel has an opening to receive liquid and the second end portion of the at least one channel has a reaction compartment and a vent opening. Accordingly, when the liquefied sample is introduced to the first end portion, capillary action assists in causing the liquefied sample to travel from the first end portion to the second end portion and at least a portion of the liquefied sample is caused to remain in the reaction compartment.
    Type: Grant
    Filed: July 26, 2004
    Date of Patent: September 1, 2009
    Inventors: Kenneth E. Smith, Haoyi Gu, Scott William Wagner, Scott Marshall Clark, Chun-Ming Chen
  • Patent number: 7550257
    Abstract: The present invention provides a process for identifying substances that modulate the activity of hyperpolarization-activated cation channels, and the use of this process.
    Type: Grant
    Filed: March 24, 2005
    Date of Patent: June 23, 2009
    Assignee: Sanofi-Aventis Deutschland GmbH
    Inventors: Hans-Willi Jansen, Andrea Brüggeman, Holger Heitsch, Heinz Gögelein
  • Patent number: 7534584
    Abstract: Particular aspects provide a method of sampling, testing and validating test lots (e.g., single-unit production lots), comprising: assembling a plurality of product portions from each of a plurality of test lots and combining the collected product portions to provide a corresponding set of test lot samples (wherein each test lot sample is attributed to a particular corresponding test lot); enriching the set of test lot samples; removing equal portions of each enriched sample, and combining the removed portions to provide a modular composite sample; and testing of the modular composite sample for the target agent/organism, wherein where such testing is positive, individual test lots may nonetheless yet be validated by further testing of a respective enriched test lot sample and obtaining a negative test result. The methods have broad utility for monitoring all sort of test lots (e.g., environmental lots, production lots, pharmaceutical lots, etc.
    Type: Grant
    Filed: August 8, 2005
    Date of Patent: May 19, 2009
    Assignee: Institute for Environmental Health, Inc.
    Inventor: Mansour Samadpour
  • Patent number: 7465560
    Abstract: A system and a method of detecting and characterizing a bacterial colony are presented in which the results are determined within about 48 hours. The bacterial colony is disposed on a substrate and placed between a laser and detector. Light from the laser impinges upon and is scattered by the bacterial colony. The forward scattered light is detected by an optical detector. The signal from the optical detector is analyzed by an analyzer and displayed or supplied to a storage medium for review. As different strains of bacteria possess unique forward scattering fingerprints, the particular strain may be identified.
    Type: Grant
    Filed: November 30, 2005
    Date of Patent: December 16, 2008
    Assignee: Purdue Research Foundation
    Inventors: Edwin D. Hirleman, Jr., Songling Guo, Arun K. Bhunia, Euiwon Bae
  • Patent number: 7361286
    Abstract: A method of detecting an etching end-point includes the steps of: forming a mask on a pattern area of an etching object; forming an etching indicator on an etching area of the etching object, which is not covered by the mask; etching the etching object using the mask; and evaluating the size of a remaining object covered by the mask using the etching indicator.
    Type: Grant
    Filed: September 8, 2005
    Date of Patent: April 22, 2008
    Assignee: Samsung Electronics Co., Ltd.
    Inventors: Young-Hyun Kim, Yu-Dong Bae, Jung-Kee Lee, In Kim
  • Patent number: 7262021
    Abstract: Media, kits, and methods are disclosed for use in processes requiring microbial culture. More specifically, the invention provides carrageenan-stabilized agar-based microbial culture media for kits and methods. The media and kits of the invention possess increased shelf-life stability over currently available agar-based media. Further, the media, kits, and methods are useful in the manual determination of identification and antimicrobial susceptibility testing of the pathogen/s contained in a specimen or sample in periods of about 12 to 24 hours. The stabilized culture media of the invention are useful in a broad variety of applications.
    Type: Grant
    Filed: January 3, 2005
    Date of Patent: August 28, 2007
    Inventor: Read Taintor
  • Patent number: 6979532
    Abstract: The present invention provides a process for identifying substances that modulate the activity of hyperpolarization-activated cation channels, and the use of this process.
    Type: Grant
    Filed: February 7, 2002
    Date of Patent: December 27, 2005
    Assignee: Aventis Pharma Deutschland GmbH
    Inventors: Hans-Willi Jansen, Andrea Brüggemann, Holger Heitsch, Heinz Gögelein
  • Patent number: 6872545
    Abstract: A microbiological analyzer for performing ID tests on samples using colorimetric techniques to generate a pixel-wise colored map of a test region of interest and also performing MIC tests on samples using nephelometric techniques to determine which antimicrobial agents are most effective against a particular microorganism.
    Type: Grant
    Filed: March 20, 2003
    Date of Patent: March 29, 2005
    Assignee: Dade Behring Inc.
    Inventors: Christopher Dallas Griner, Thomas Kenneth McDowell
  • Publication number: 20040197850
    Abstract: The present invention generally provides an improved transfer film having multiple layers for laser micro-capture of a sample. The transfer film for laser micro-capture includes distinct layers for expansion and adhesion in order to optimize the performance of the transfer film. A transfer film including a spring-back layer is also disclosed.
    Type: Application
    Filed: April 19, 2004
    Publication date: October 7, 2004
    Inventors: Thomas M. Baer, Robert H. Reamey, David F Head, Barbara J. Weesen-Baer, Ali Firouzi, David Ching
  • Patent number: 6764832
    Abstract: A solid growth plating medium in which Shigella organisms will grow and form colonies in the medium, and substantially, other microorganisms are inhibited or their colonies are differentiated from Shigella organisms. In one embodiment of the invention, colonies produced by Shigella appear with the color of the plating medium, usually a clear off-white color, that can be readily observed. In another embodiment, the fact that Shigella boydii and Shigella sonnei produce the enzyme alpha-galactosidase, but most Shigella dysenteriae and Shigella flexneri strains do not, is utilized with a chromogenic substrate to produce colonies of these microorganisms of a distinguishing color.
    Type: Grant
    Filed: August 22, 2001
    Date of Patent: July 20, 2004
    Inventor: Lawrence Restaino
  • Patent number: 6656729
    Abstract: The present invention is to methods of obtaining plant-derived compositions that inhibit apoptosis, the compositions obtained thereby, compositions comprising the composition, and methods of use thereof.
    Type: Grant
    Filed: October 23, 2001
    Date of Patent: December 2, 2003
    Assignee: Sky High, LLC
    Inventors: Ian Cyril Bathurst, John D. Bradley, L. David Tomei, Philip J. Barr
  • Patent number: 6617149
    Abstract: An isolation plating medium for use in processes for the presumptive identification of Escherichia coli 0157:H7 from a sample that also contains other strains of Escherichia coli. The plating medium comprises at least one carbohydrate that Escherichia coli 0157:H7 is incapable of fermenting, but other strains of Escherichia coli do ferment said carbohydrate, a pH indicator dye that changes the color of the plating medium to a first color when the pH of the medium changes, a chromogenic substrate that reacts to beta-galactosidase to form a precipitate in the plating medium of a second color which contrasts with the first color.
    Type: Grant
    Filed: March 18, 2002
    Date of Patent: September 9, 2003
    Assignee: R&F Laboratories, Inc.
    Inventor: Lawrence Restaino
  • Publication number: 20030124643
    Abstract: A Method and Kit for performing concurrent identification testing and antimicrobial susceptibility testing from broth culture (90) are described. Broth (82) incubation is generally 4 to 6 hrs providing adequate numbers of microorganisms for inoculating a multi-chambered kit plate (80) comprising enriched, differential, selective, differential-selective, single-purpose and susceptibility media. Several dilutions are prepared from the cultured broth, for inoculation of the kit plate (80). The more dilute concentration (140) produces individual colonies of microorganisms, for identification testing. This isolation makes an initial isolation step unnecessary. The heavier concentration dilution (96) provides inoculation for antimicrobial susceptibility tests and other identification tests. In addition, antimicrobial susceptibilities are shown valid even when several different microorganisms coexist in the same test chamber.
    Type: Application
    Filed: December 3, 2001
    Publication date: July 3, 2003
    Inventor: Read Robert Taintor
  • Publication number: 20030049718
    Abstract: A solid growth plating medium in which Shigella organisms will grow and form colonies in the medium, and substantially, other microorganisms are inhibited or their colonies are differentiated from Shigella organisms. In one embodiment of the invention, colonies produced by Shigella appear with the color of the plating medium, usually a clear off-white color, that can be readily observed. In another embodiment, the fact that Shigella boydii and Shigella sonnei produce the enzyme alpha-galactosidase, but most Shigella dysenteriae and Shigella flexneri strains do not, is utilized with a chromogenic substrate to produce colonies of these microorganisms of a distinguishing color.
    Type: Application
    Filed: August 22, 2001
    Publication date: March 13, 2003
    Inventor: Lawrence Restaino
  • Publication number: 20030044882
    Abstract: The present invention is related with the Microbiology field and particularly with a composition and a method for early detection, identification, differentiation and count of microscopic organisms, concretely Gram-negative microorganisms.
    Type: Application
    Filed: April 4, 2002
    Publication date: March 6, 2003
    Inventors: Vivian de Jesus Quesada Muniz, Claudio Rodriguez Martinez
  • Patent number: 6429025
    Abstract: The present invention provides novel microfluidic devices and methods that are useful for performing high-throughput screening assays. In particular, the devices and methods of the invention are useful in screening large numbers of different compounds for their effects on a variety of chemical, and preferably, biochemical systems.
    Type: Grant
    Filed: December 22, 1998
    Date of Patent: August 6, 2002
    Assignee: Caliper Technologies Corp.
    Inventors: John Wallace Parce, Anne Kopf-Sill, Luc J. Bousse
  • Publication number: 20020090668
    Abstract: A test medium and method for detecting, quantifying, identifying and differentiating up to four (4) separate biological materials in a test sample. A test medium is disclosed which allows quantifying and differentiating under ambient light aggregates of biological entities producing specific enzymes, which might include general coliforms, E. coli, Aeromonas, and Salmonella or Shigella in a single test medium. A new class of nonchromogenic substrate is disclosed which produce a substantially black, non-diffusible precipitate. This precipitate is not subject to interference from other chromogenic substrates present in the test medium. In a preferred form, the substrates are selected such that E. coli colonies present in the test medium show as substantially black, general coliforms colonies show in the test medium as a blue-violet color, Aeromonas colonies present in the test medium show as a generally red-pink color, and Salmonella or Shigella colonies show as a generally teal-green color.
    Type: Application
    Filed: January 7, 2002
    Publication date: July 11, 2002
    Inventors: Geoffrey N. Roth, Jonathan N. Roth
  • Publication number: 20020086344
    Abstract: A method for classifying and counting nucleated bone marrow cells comprises the steps of: (1) mixing a sample of bone marrow fluid with an erythrocyte lysing agent to lyse erythrocytes in the sample and render leukocytic cells and erythroid cells in the sample suitable for staining, and staining the sample with a fluorescent dye for producing a difference in intensity of fluorescence between the leukocytic cells and the erythroid cells; (2) introducing the resulting sample to a flow cytometer to detect at least one kind of scattered light and at least one kind of fluorescence; (3) classifying and counting nucleated bone marrow cells, the leukocytic cells and the erythroid cells with use of a difference in the intensity of the fluorescence and the scattered light; (4) calculating the ratio of the nucleated bone marrow cells to the erythroid cells or leukocytic cells from the obtained erythroid cell count or leukocytic cell count and the obtained nucleated bone marrow cell count; and (5) calculating the ratio o
    Type: Application
    Filed: November 6, 2001
    Publication date: July 4, 2002
    Applicant: Sysmex Corporation
    Inventors: Tomohiro Tsuji, Yusuke Mori, Takashi Sakata, Yukio Hamaguchi
  • Patent number: 6387708
    Abstract: Formed constituents in an aqueous based fluid biologic material sample are separated from the aqueous constituent of the sample, and are concentrated in an examining instrument's focal plane where they can be examined under magnification. Examples of fluids that can be analyzed in this fashion include urine; cerebrospinal fluid; pleural fluid; ascites; fluids aspirated from cysts such as thyroid and breast cysts; cytologic specimens which have been placed in an aqueous fluid; platelet-rich plasma; and the like. The sample is placed in a chamber having a layer of a hydrophilic hydrogel covering a surface of the chamber. An opposite surface of the chamber is transparent, and may be formed by a microscope slide cover slip, or the like. The volume of hydrogel in the chamber is sufficient so that, when the hydrogel absorbs essentially all of the aqueous fraction of the sample, the hydrogel will expand and fill the chamber.
    Type: Grant
    Filed: June 21, 2001
    Date of Patent: May 14, 2002
    Assignees: Wardlaw Partners, LLP
    Inventor: Stephen C. Wardlaw
  • Patent number: 6383820
    Abstract: A multi-slide assembly includes various components that alone or in combination facilitate testing of test samples with minimal manipulation of assay components. Moreover, the various device components permit centrifugation, culturing and analysis of each test sample to be performed in the same assembly. A frame retains a plurality of reaction vessel assemblies between a pair of opposing channels that are configured to slidably receive the opposing ends of each reaction vessel assembly. A strip cap seals the openings in each reaction vessel using cap members having sealing rings circumscribing the external walls thereof to form compression seals with internal walls of the reaction vessels. Furthermore, in a method of making a multi-well slide assembly, an ultrasonic welding process removably bonds a plurality of wells to a slide plate to provide an adequate seal therebetween during centrifugation, yet still enable separation thereof by an operator.
    Type: Grant
    Filed: February 22, 2000
    Date of Patent: May 7, 2002
    Assignee: Nalge Nunc International Corporation
    Inventors: Kenneth E. Bunn, Abdul Wahid Khan, Susan K. W. Nanda, Barbara Myszkiewicz Sullivan, Keith O. Whittlinger, Kathleen R. Williamson, Hoyt E. Matthai
  • Patent number: 6365368
    Abstract: The present invention concerns methods of testing water for microbe contamination. The methods of the invention comprise supplementing existing methods with assays using specific reagents such as monoclonal antibodies. The invention also concerns a device for use in the methods of the invention.
    Type: Grant
    Filed: December 7, 1992
    Date of Patent: April 2, 2002
    Assignee: IGEN International, Inc.
    Inventors: Scott A. Minnich, Steven A. Lobel, Gerald Schochetman, Peter Feng, Richard Massey
  • Publication number: 20020028480
    Abstract: Drug candidate screening methods are applied to discover compounds with activity against ion channel targets. The method may include modulating the transmembrane potential of host cells in a plurality of sample wells with a repetitive application of electric fields so as to set the transmembrane potential to a level corresponding to a pre-selected voltage dependent state of a target ion channel.
    Type: Application
    Filed: March 12, 2001
    Publication date: March 7, 2002
    Inventors: Michael P. Maher, Jesus E. Gonzalez
  • Publication number: 20020019023
    Abstract: The present invention relates to methods for high information content (HIC) analysis or screening of complex biological systems using Fourier transform mass spectrometry (FTMS). The present methods are useful for analyzing complex biological mixtures containing both high molecular weight molecules (e.g., polynucleotides, proteins, polysaccharides) and low molecular weight molecules (e.g., oligonucleotides, peptides, lipids, oligosaccharides, steroid hormones, catabolic and metabolic intermediates) permit the elucidation of molecular differences between complex biological samples, and permit the identification of biologically active molecules (e.g. therapeutically active drugs, etc.).
    Type: Application
    Filed: December 12, 2000
    Publication date: February 14, 2002
    Inventors: Jean-Louis H. Dasseux, Roger S. Newton, Thomas J. Rea, Charles L. Bisgaier, Michael E. Pape
  • Patent number: 6204048
    Abstract: A mutant Pseudomonas ayucida strain No PTA-806 which is able to selectively cleave organic C—N bonds and reduce the nitrogen content of organic carbonaceous materials is described.
    Type: Grant
    Filed: November 30, 1999
    Date of Patent: March 20, 2001
    Assignee: Petroleo Brasileiro S.A-Petrobras
    Inventors: John J. Kilbane, II, Claudia Maria Soares Ribeiro, Mônica Moreira Linhares
  • Patent number: 6184043
    Abstract: The invention relates to a method for detecting specific target-cells in a simple and time saving way, using paramagnetic particles, antibodies recognizing the Fc portions of target-cell associating antibodies and target-cell associating antibodies directed to specific antigen determinants in the target-cell membranes. Incubation of the cell suspension with a mild detergent and/or second set of antibodies or antibody fragments, prelabeled or not with fluorescent agents, metallocolloids, radioisotopes, biotincomplexes or certain enzymes allowing visualization, with dramatically increase the specificity of the method. The method can further be used for isolation of the target-cells by magnetic field application and kit for performing the method according to the invention is described.
    Type: Grant
    Filed: June 24, 1997
    Date of Patent: February 6, 2001
    Inventors: Øystein Fodstad, Gunnar Kvalheim
  • Patent number: 6087156
    Abstract: A solid plating medium for the presumptive detection of Escherichia coli 0157:H7 which comprises (1) an ingredient which promotes growth of Escherichia coli cells under incubation, (2) an ingredient which inhibits growth of gram positive microorganisms under incubation, (3) an ingredient that inhibits growth of Proteus sp.
    Type: Grant
    Filed: October 23, 1998
    Date of Patent: July 11, 2000
    Assignee: R&F Laboratories, Inc.
    Inventor: Lawrence Restaino
  • Patent number: 6022682
    Abstract: An article for detecting thermostable nuclease positive, potentially enterotoxigenic, staphylococci, containing unhydrolyzed nucleotides, toluidine blue O, and a binder, wherein the article is adapted for placement against a sample suspected of containing enterotoxigenic staphylococci. A method of detecting thermostable nuclease positive staphylococci in a sample utilizing the article, and a kit for the detection of thermostable nuclease positive staphylococci containing the article, are also described.
    Type: Grant
    Filed: August 14, 1996
    Date of Patent: February 8, 2000
    Assignee: Minnesota Mining and Manufacturing Company
    Inventors: Patrick A. Mach, Marlys E. Lund
  • Patent number: 6020150
    Abstract: A method for detecting the presence or absence of microorganisms belonging to the group which consists of bacteria and yeasts in a liquid sample (5) of a biological material. The method comprises placing the liquid sample (5) in a centrifuge tube (6a, 6b) above a gelled system (10) comprising at least (a) a first so-called development phase (1), i.e. a gel comprising a microorganism culture medium and a reagent for inducing a detectable optical measurement change in the presence of microorganisms, said gel being an intimate mixture of water and water-absorbing polymeric particles that have swelled in such a way that, in said intimate mixture, said polymeric particles have (.alpha.) a dry weight concentration of 0.05-0.2 g/ml, and (.beta.) a swollen-state diameter of 90-320 .mu.
    Type: Grant
    Filed: September 22, 1997
    Date of Patent: February 1, 2000
    Assignee: Diffusion Bacteriologie Du Var
    Inventors: Genevieve Contant-Pussard, Fran.cedilla.oise Boisard-Beaupere, Jean-Luc Martinoli, Alain Rousseau
  • Patent number: 5876959
    Abstract: A method for detecting, identifying and measuring infectious disease causing microorganisms is described. The method utilizes a plural medium arrangement in which a test liquid containing a microorganism is simultaneously introduced. Different media are utilized for the plural medium arrangement and at least one of the media can contain a respiration inhibiting material and/or other reagents such as antibiotics. Dissolved oxygen measurements of the media are taken as a measurement of microorganism respiration. Oxygen electrode signals within different media are compared and differential respiration variation rates are determined and identification signals produced. The identification signal resulting from the multiple respiration rate determinations is compared with known values to identify and measure the microorganism. The drug sensitivity of a microorganism is also judged when at least one of the media contains a drug for which the sensitivity of microorganism is to be determined.
    Type: Grant
    Filed: April 29, 1996
    Date of Patent: March 2, 1999
    Assignee: Daikin Industries, Ltd.
    Inventors: Shin-ichiro Kusunoki, Jun-ichiro Arai
  • Patent number: 5824494
    Abstract: A method for determining the number of microorganisms in a liquid sample, including the steps of dividing the liquid sample into a plurality of separate sub-samples, measuring the magnitude of at least one impedance component across each of the sub-samples and determining the number of the sub-samples for which the measured magnitude of the at least one impedance component indicates microorganism growth. The method preferably further includes the steps of applying A.C. voltage at a plurality of frequencies across at least a portion of the sample and measuring the at least one impedance component in response to at least some of the plurality of A.C. frequencies.
    Type: Grant
    Filed: April 26, 1995
    Date of Patent: October 20, 1998
    Assignee: Sirotech Ltd.
    Inventor: Simon Feldberg
  • Patent number: 5700655
    Abstract: Method for detection of a biological material in a sample. The method includes the steps of liquifying the sample (if necessary) and pouring the liquified sample into the incubation plate. The incubation plate has a generally flat horizontal surface and the surface is divided into a plurality of at least 20 recessed wells. Each well is adapted to hold an aliquot of liquid and is sized and shaped, and formed of a suitable material, to hold the aliquot within the well by surface tension. Any excess liquid from the liquified sample is poured from the surface of the plate. The method then involves incubating that incubation plate until the presence or absence of the biological material is determined.
    Type: Grant
    Filed: February 23, 1996
    Date of Patent: December 23, 1997
    Assignee: Idexx Laboratories, Inc.
    Inventors: Andrew J. Croteau, Mark W. Pierson, David E. Townsend, Ali Naqui
  • Patent number: 5641753
    Abstract: Pharmaceutical compositions of an erythromycin derivative combined with sulfisoxazole according to the structural formulas: ##STR1## where R is hydrogen, C1-C10 alkylcarbonyl or C1-C10 alkyl wherein the substituent is amino or cyano; R1 and R2 are independently hydrogen, hydroxyl or amino; and the pharmaceutical salts and esters thereof.
    Type: Grant
    Filed: March 25, 1996
    Date of Patent: June 24, 1997
    Assignee: Chris E. Platt
    Inventor: Chris Platt
  • Patent number: RE40835
    Abstract: A method for restoring immunoreactivity of a tissue, particularly decalcified tissue, fixed width an aldehyde fixative agent and embedded in an embedding medium, usually comprising celloidin, the method comprising the steps of contacting the tissue with an aldehyde releasing reagent solution comprising a solvent and an aldehyde releasing reagent and removing aldehyde released by the aldehyde releasing reagent from contact with the tissue by reacting the aldehyde in a substantially irreversible manner to form a non-aldehyde derivative, and removing excess base from the tissue. A preferred solution for celloidin-embedded decalcified tissue comprises methanolic sodium hydroxide at about one-third saturation.
    Type: Grant
    Filed: August 12, 1993
    Date of Patent: July 7, 2009
    Assignee: BioGenx Laboratories, Inc.
    Inventors: Shan-Rong Shi, Atul K. Tandon, Krishan L. Kalra, Nagesh Malhotra, Sheng-Hui Su, Cheng-Zhi Yu
  • Patent number: RE43979
    Abstract: The invention relates to a method for detecting specific target-cells in a simple and time saving way, using paramagnetic particles, antibodies recognizing the Fc portions of target-cell associating antibodies and target-cell associating antibodies directed to specific antigen determinants in the target-cell membranes. Incubation of the cell suspension with a mild detergent and/or second set of antibodies or antibody fragments, prelabeled or not with fluorescent agents, metallocolloids, radioisotopes, biotincomplexes or certain enzymes allowing visualization, with dramatically increase the specificity of the method. The method can further be used for isolation of the target-cells by magnetic field application and kit for performing the method according to the invention is described.
    Type: Grant
    Filed: February 5, 2003
    Date of Patent: February 5, 2013
    Assignee: Abbott Laboratories
    Inventors: Oystein Fodstad, Gunnar Kvalheim